1. Genetic analysis of embryo in a human case of spontaneous oocyte activation: a case report.
- Author
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Ye Y, Li N, Yan X, Wu R, Zhou W, Cheng L, and Li Y
- Subjects
- Adult, Biopsy, Blastomeres chemistry, Blastomeres metabolism, DNA Copy Number Variations, Embryo, Mammalian chemistry, Embryo, Mammalian metabolism, Embryo, Mammalian pathology, Female, Fertilization in Vitro, Genetic Testing, Humans, Infertility, Female genetics, Infertility, Female pathology, Infertility, Female therapy, Sperm Injections, Intracytoplasmic, Treatment Failure, Blastomeres pathology, Oocytes physiology, Parthenogenesis genetics, Preimplantation Diagnosis
- Abstract
Parthenogenesis, a unique form of reproduction, is normally inhibited in mammals and a human embryo with parthenogenetic origin is not considered capable of producing offspring. The aim of this report is to analyze a parthenogenetic oocyte retrieved from a patient so as to have a better understanding on parthenogenesis and causes of infertility. A 38-year-old woman presented at our center with a history of primary infertility for 10 years and underwent an IVF-ICSI cycle. Three MII oocytes retrieved and one of which presented with 1 pronucleus before conducting ICSI and developed into an embryo 30 h post-retrieval. Blastomere biopsy, genome amplification, copy number variation (CNV) analysis and MultiSNPs analysis was performed on the embryo. The results showed that only one blastomere contains DNA and CNV analysis indicated a genotype of 48, XX, +17, +17 and the genetic contribution of biopsied embryo was of exclusively maternal origin. Such analysis might be beneficial for patients with a history of oocyte spontaneous activation in diagnosing case-specific aberrations and providing individualized therapeutic strategies such as preimplantation genetic diagnosis to choose a genetic normal embryo to transplant.
- Published
- 2020
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