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Purpose: Under-foot impact loadings can cause serious lower limb injuries in many activities, such as automobile collisions and underbody explosions to military vehicles. The present study aims to compare the biomechanical responses of the mainstream vehicle occupant dummies with the human body lower limb model and analyze their robustness and applicability for assessing lower limb injury risk in under-foot impact loading environments. Methods: The Hybrid III model, the test device for human occupant restraint (THOR) model, and a hybrid human body model with the human active lower limb model were adopted for under-foot impact analysis regarding different impact velocities and initial lower limb postures. Results: The results show that the 2 dummy models have larger peak tibial axial force and higher sensitivity to the impact velocities and initial postures than the human lower limb model. In particular, the Hybrid III dummy model presented extremely larger peak tibial axial forces than the human lower limb model. In the case of minimal difference in tibial axial force, Hybrid III's tibial axial force (7.5 KN) is still 312.5% that of human active lower limb's (2.4 KN). Even with closer peak tibial axial force values, the biomechanical response curve shapes of the THOR model show significant differences from the human lower limb model. Conclusion: Based on the present results, the Hybrid III dummy cannot be used to evaluate the lower limb injury risk in under-foot loading environments. In contrast, potential improvement in ankle biofidelity and related soft tissues of the THOR dummy can be implemented in the future for better applicability.

A forest is vulnerable to drought and plays important roles in the regulation of carbon and water cycling in a terrestrial ecosystem. Atmospheric vapor pressure deficit (VPD) has been identified as an increasingly major factor in plant functioning and has been established as a main contributor to recent drought-induced plant mortality, independent of other drivers associated with climate change. However, most previous studies have focused on the effects of climate warming and CO2 enrichment on vegetation growth, without considering the effects of an increased VPD on vegetation growth and evapotranspiration (ET) in forest ecosystems. This could lead to a large uncertainty in estimating the variability in forest carbon sinks. Based on the long-term satellite data, we investigated the response of the leaf area index (LAI) and ET to the VPD via a partial correlation analysis in this study. We also examined the temporal variability in the partial coefficients within a ten-year moving window. The results showed that over 50% of the region displayed a negative partial correlation between the LAI, ET, and VPD, and those pixels were mainly concentrated in North America and the plains of Eastern Europe. Regions with a negative trend of partial correlation in both the LAI and ET are mostly located in the plains of Eastern Europe and the Siberian Plain of western Russia, while the positive trend is mainly in South America. The plains of Eastern Europe are becoming drier, which was proved by the interannual trend of the Standardized Precipitation Evapotranspiration Index (SPEI) and soil water content (SWC). Additionally, the LAI and ET in those areas exhibited a significant positive correlation with the SWC based on the moving window average. This study suggests that the role of the VPD on vegetation will become increasingly prominent in the context of future climate change for the forest ecosystem.

PurposeThis study aimed to investigate the value of quantified extracellular volume fraction (fECV) derived from dual-energy CT (DECT) for predicting the survival outcomes of patients with hepatocellular carcinoma (HCC) after transarterial chemoembolization (TACE).Materials and methodsA total of 63 patients with HCC who underwent DECT before treatment were retrospectively included. Virtual monochromatic images (VMI) (70 keV) and iodine density images (IDI) during the equilibrium phase (EP) were generated. The tumor VMI-fECV and IDI-fECV were measured and calculated on the whole tumor (Whole) and maximum enhancement of the tumor (Maximum), respectively. Univariate and multivariate Cox models were used to evaluate the effects of clinical and imaging predictors on overall survival (OS) and progression-free survival (PFS).ResultsThe correlation between tumor VMI-fECV and IDI-fECV was strong (both p< 0.001). The Bland–Altman plot between VMI-fECV and IDI-fECV showed a bias of 5.16% for the Whole and 6.89% for the Maximum modalities, respectively. Increasing tumor VMI-fECV and IDI-fECV were positively related to the effects on OS and PFS (both p< 0.05). The tumor IDI-fECV-Maximum was the only congruent independent predictor in patients with HCC after TACE in the multivariate analysis on OS (p = 0.000) and PFS (p = 0.028). Patients with higher IDI-fECV-Maximum values had better survival rates above the optimal cutoff values, which were 35.42% for OS and 29.37% for PFS.ConclusionThe quantified fECV determined by the equilibrium-phase contrast-enhanced DECT can potentially predict the survival outcomes of patients with HCC following TACE treatment.

Summary: Ground-level ozone threatens rice production, which provides staple food for more than half of the world’s population. Improving the adaptability of rice crops to ozone pollution is essential to ending global hunger. Rice panicles not only affect grain yield and grain quality but also the adaptability of plants to environmental changes, but the effects of ozone on rice panicles are not well understood. Through an open top chamber experiment, we investigated the effects of long-term and short-term ozone on the traits of rice panicles, finding that both long-term and short-term ozone significantly reduced the number of panicle branches and spikelets in rice, and especially the fertility of spikelets in hybrid cultivar. The reduction in spikelet quantity and fertility because of ozone exposure is caused by changes in secondary branches and attached spikelet. These results suggest the potential for effective adaptation to ozone by altering breeding targets and developing growth stage-specific agricultural techniques.

The major challenge in the field of antibacterial agents is to overcome the low-permeability of bacteria cell membranes that protects the cells against diverse drugs. In this work, water-soluble polyaniline (PANI)-poly (p-styrenesulfonic acid) (PSS) (PANI:PSS) is found to spontaneously penetrate bacteria cellular membranes in a non-disruptive way, leaving no evidence of membrane poration/disturbance or cell death, thus avoiding side effects caused by cationic ammonia groups in traditional ammonia-containing antibacterial agents. For aqueous synthesis, which is important for biocompatibility, the polymer is synthesized via an enzyme-mimetic route relying on the catalysis of a nanozyme. Owing to its fluorescent properties, the localization of as-prepared PANI:PSS is determined by the confocal microscope, and the results confirm its rapid entry into bacteria. Under 808 nm near-infrared (NIR) irradiation, the internalized PANI:PSS generates local hyperthermia and destroys bacteria highly efficiently from inside the cells due to its excellent photothermal effects. Staphylococcus aureus (S. aureus), Methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli) could be effectively eliminated as well as the corresponding bacterial biofilms. Results of in vivo antibacterial experiments demonstrate excellent antibacterial activities of the water-soluble PANI:PSS without side effects. Therefore, the prepared water-soluble polymer in this study has great potential in the treatment of various bacterial infections.

Application of atoxigenic strains of Aspergillus flavusto soils is the most successful aflatoxin biological control approach. The objective of this study was to evaluate the efficacies of native non-aflatoxin producing (atoxigenic) strains as a biocontrol agent in peanut field in China. The competitive atoxigenic A. flavus strains (JS4, SI1and SXN) isolated from different crops, in China were used for field evaluation. The strains applied during the growing season (June – October, 2016) in the field at rate of 25 kg inoculum/hectare. The colonization of these biocontrol agents has been investigated and the population of A. flavus communities in soil were determined. The incidences of toxin producing (toxigenic) A. flavus strains and aflatoxin contamination in peanuts were also determined. Treated plots produced significant reductions in the incidence of toxigenic isolates of A. flavus in soil. However, the total fungal densities were not significantly different (p > 0.05) after treatments. Large percentage of aflatoxin reductions, ranging from 82.8% (SXN) up to 87.2% (JS4) were recorded in treated plots. Generally, the results suggest that the strategy can be used to control aflatoxin contamination and continuous evaluation should be done.

High concentration of tropospheric ozone (O3) causes crop yield losses, which could be reduced by foliar application of ethylenediurea (EDU). Rice grain appearance is a major quality trait that determines the milling quality, white rice productivity and the market value. Grain chalkiness is one of the common defects that deteriorate the grain appearance in rice due to its negative effects on palatability and milling yield. Whether EDU could reduce grain chalkiness in rice which was usually increased by high concentration of O3 is not clarified. We report the grain chalkiness in 19 rice cultivars (CVs) of three types: indica (6 CVs), japonica (5 CVs) and hybrids (8 CVs), observed in an EDU application experiment in the field in China. The ambient O3 level as expressed by accumulated hourly O3 concentration over the threshold of 40 ppb (AOT40) for 80 days until maturity reached 12.8 ppm h at a near-by monitoring station. Fraction of the chalky grains (FCG) in the hybrid cultivars was 8% lower in EDU than that in the control treatments, whereas no significant effect of EDU on FCG was found in japonica or indica cultivars. The reduction of FCG due to EDU treatment in hybrid cultivars was attributed to the significant reduction of milky white grains followed by that of white belly grains. Thus, the application of EDU could ameliorate the decline of grain appearance quality in hybrid rice by decreasing the FCG and enhancing the fraction of perfect grains (FPG). Moreover, there were significant interactions between the EDU application and rice cultivars, indicating varietal difference in the protection of grain appearance quality by EDU. These results suggest the need for further studies on the mechanisms of the effects of EDU on grain chalkiness.

Abstract Background Hypertension is the the primary cause of diastolic heart failure. Oxidative stress plays an important role in cardiac diastolic dysfunction caused by hypertension. The occurrence of oxidative stress is related to the level of nitric oxide (NO) in the body. Tetrahydrobiopterin (BH4) is an essential cofactor for NO synthesis. Nebivolol can reduce myocardial oxidative stress and increase NO activity. Therefore, we investigated the effects of monotherapy or combination therapy of different doses of BH4 and nebivolol on cardiac diastolic function in spontaneously hypertensive rats, and preliminarily expounded the related mechanisms. Methods Left ventricular function was evaluated by non-invasive echocardiographic assessment and invasive right carotid artery catheterization methods. ELISA was used to measure myocardial 3-nitrotyrosine content, NO production, and cyclic guanosine monophosphate (cGMP) concentration in the myocardium; quantitative real-time PCR (qRT-PCR) was used to determine endothelial nitric oxide synthase (eNOS), phospholamban and sarcoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) mRNA expression levels; Western blot was used to detect the protein expression levels of eNOS and eNOS dimers in myocardial tissue, and immunohistochemical detection of cGMP expression in the myocardium was performed. Results Studies have shown that compared with those in the control group, NO generation and the expression level of myocardial eNOS mRNA, eNOS expression of dimers, phospholamban, SERCA2a and cGMP increased significantly after the combined intervention of BH4 and nebivolol, while the expression of 3-nitrotyrosine was significantly decreased. Conclusions The combined treatment group had a synergistic effect on reducing myocardial oxidative stress, increasing eNOS content, and increasing NO production, and had a more obvious protective effect on diastolic dysfunction through the nitric oxide/cyclic guanosine monophosphate (NO/cGMP) pathway.

The article considers improvement of the methodology for accounting for the degradation of asphalt concrete working in the upper layers of the pavement. Development of recycling technologies for road structures is an ongoing process; it allows reaching a higher quality of reclaimed materials and using them for subsequent construction of structural layers, including the upper layers without the protective ones, as well as during repair and reconstruction of roads of various technical categories. At the same time, the system of pre-project assessment (diagnostics) of the state of asphalt concrete pavements cannot be considered optimal and effective because the determined indicators demonstrate that, firstly, various surface and structural defects are present, and, secondly, that the indicators mentioned above are more relevant to the road structure as a whole. The joint handling of the theoretical and experimental data allows concluding that damageability level depends on the physical, mechanical and structural properties, the main being maximal structural strength and the number of elastic bonds involved in the deformation process. A variant of modelling of asphalt concrete damageability depending on the work capacity is proposed, when the reduced amount of dissipated energy is replaced with sufficient accuracy for practice by the ratio of the actual number of load application cycles (freezing and thawing cycles) to the limit. A correlation between the level of damageability and the kinetics of changes of the interpore space of asphalt concrete under the influence of strain (temperature, climatic factors) has been established. Results allow fixing (predicting) the level of damageability by measuring the level of water permeability. The research methodology and equipment for implementation thereof was developed earlier, it can be effectively used at the stage of pre-project diagnosis.

The article addresses the issues of structure formation of road composite materials containing hydraulic (рortland cement) and organic (bitumen) binders. It has been determined that organic and hydraulic binders, being thermodynamically incompatible, are capable of interaction and complement each other. Structure formation processes are associated with interphase transition layers interaction mechanism and the direct formation of phase contacts with cement crystallohydrates. The interphase boundary is diffuse and is established through interphase transition layers. The emergence of interfacial layers is thermodynamically advantageous, since it contributes to a decrease in Gibbs free energy and does not contradict modern concepts of solid state physics. It was established that with cement content of about 30 % of complex bitumen-cement binder volume, there will appear (nucleate) phase contacts that will prevail in the binder structure when the cement content is more than 60 %. In the case phase contacts prevail, concrete will demonstrate significant strength at high temperatures, but low temperature and fatigue crack resistance, which will lead to their durability loss. The cement content of 30–40 % of the total complex binder can be considered optimal.

Abstract Rising concentration of surface O3 threatens crop production and food security. To improve the evaluation and develop efficient adaptations, it is essential to study the effects of acute O3 pollution. We adopted this study by open‐top chamber (OTC) method and found that the fumigation of elevated O3 (NF40) for short (tillering and jointing, heading and ripening) and long periods during rice growth reduced the grain yield by 17, 19 and 25%, respectively, showing that both of the acute and chronic O3 pollution reduce rice production. Moreover, in comparison with the plants under chronic O3 pollution, grain yield was increased when O3 was removed at different stages: 32% increment at tillering and jointing and 34% increment at heading and ripening. These results suggest a possibility to recover the yield loss in rice under O3 pollution.

Two-dimensional materials (2DMs) have attracted substantial attention due to their abundant active sites and their ultrahigh surface area for different catalytic applications due to the high lateral-longitudinal ratio. Transition metal dichalcogenides (TMDs), especially MoS2, as one of the 2DMs most often studied, have shown superior activity in electrochemical applications. Recently, combinations of different 2DMs have been widely studied, and they appear to be the most promising strategy available to develop state of the art catalysts for different reactions.In this article, we review the interactions between MoS2 and other materials as well as the novel assembly induced phase transitions of TMDs and their underlying mechanisms. Several methods for inducing the phase transition of TMDs by building MoS2-based heterostructures have been introduced. The electronic coupling between these counterparts has significantly enhanced their conductivity and optimized the energy states of the materials, thus introducing enhanced activity as compared to their original counterparts. The ideas summarized in this article may shed new light on and help to develop next-generation green energy materials by designing and constructing highly active two-dimensional catalysts for efficient water splitting. Keywords: Molybdenum disulfide, Heterostructure, Phase transition, Interaction, Water splitting

Rice quality changes during storage. However, few studies have reported the difference in protein structure between the indica and japonica varieties of rice during storage. The current research characterized the structural properties of the rice protein, and further investigated the proteomic profiles of Jianzhen 2 (indica rice) and Nanjing 9108 (japonica rice) during storage using the TMT labeling method. A significant reduction in free sulfhydryl content and an increase in disulfide bonds content and surface hydrophobicity were observed in both varieties after storage. The results of FTIR indicated that the changes in the protein’s secondary structure of Nanjing 9108 (japonica rice) were more significant than in Jianzhen 2 (indica rice). A total of 4039 proteins in Nanjing 9108 and 4301 proteins in Jianzhen 2 were identified by TMT-labeled proteomics analysis in this study. Significantly, changes were detected in 831 proteins in Nanjing 9108, while only in 60 proteins in Jianzhen 2. Protein processing in endoplasmic reticulum, starch, and sucrose metabolism were both accelerated in both varieties, while oxidative phosphorylation in mitochondria, glycolysis, fatty acid metabolism, and glutathione metabolism were enhanced in Nanjing 9108 (japonica rice). This study provides insight into the proteomic changes and protein structure in rice induced by storage.

Ethylenediurea (EDU) is an indicator of surface ozone (O3), has a high potential to be developed as an applicable protectant for crops against O3 phytotoxicity. Studies on the effects of EDU on grain quality are few, limiting evaluation of the efficiency of EDU protection. In order to understand the effects of EDU on grain quality in rice, a field study was conducted in a rice paddy, where EDU solutions were foliar applied to rice plants. At maturity, grain nitrogen concentrations (GN) in 21 rice cultivars and related traits were analyzed. Mean across 21 cultivars, GN was reduced by EDU by 3.81%, suggesting that O3 in ambient air is affecting grain quality. GN negatively correlated with grain weight and source/sink ratio, but positively correlated with spikelet density. Moreover, GN changes to EDU were rice type dependent, which were −0.43%, −0.72%, and 1.19% in indica, japonica, and hybrid rice, respectively. These results suggest that EDU promotes sink in rice, which helps to increase grain yield, but allocation of nitrogen is not enough to maintain GN in both indica and japonica cultivars. Rice types and cultivars’ variations in the responses of GN to EDU highlight a possibility to adjust grain quality by EDU, combining cultivar selection and agricultural management in response to surface O3 pollution.

To determine the changes in the quality of rice during storage, this study investigated the comprehensive metabolomic profiles of Nanjing 9108 (typical japonica rice) and Jianzhen 2 (typical indica rice) varieties in China, using metabolomics. A total of 13 categories of 593 metabolites including lipids (134 species), phenolic acids (78 species), flavonoids (70 species), alkaloids (67 species), organic acids (64 species), amino acids and derivatives (64 species), saccharides and alcohols (44 species), nucleotides and derivatives (37 species), vitamins (14 species), lignans and coumarins (9 species), tannins (2 species), terpenoids (2 species), and others (8 species) were identified in both varieties. The result showed significant changes in 204 metabolites in Nanjing 9108, while only 26 were altered in Jianzhen 2 during storage. These metabolites involved 46 metabolic pathways. The TCA cycle, linoleic, and α-linolenic acid metabolic pathways were unique in Nanjing 9108. Finally, the results of quantitative mass spectrometry of 11 metabolites provided insight into biomarkers associated with quality deterioration of rice. This study provides insights into the mechanism of deterioration in the quality of rice during storage.

N-doped graphene samples with different N species contents were prepared by a two-step synthesis method and evaluated as electrocatalysts for the nitrate reduction reaction (NORR) for the first time. In an acidic solution with a saturated calomel electrode as reference, the pyridinic-N dominant sample (NGR2) had an onset of 0.932 V and a half-wave potential of 0.833 V, showing the superior activity towards the NORR compared to the pyrrolic-N dominant N-doped graphene (onset potential: 0.850 V, half-wave potential: 0.732 V) and the pure graphene (onset potential: 0.698 V, half-wave potential: 0.506 V). N doping could significantly boost the NORR performance of N-doped graphene, especially the contribution of pyridinic-N. Density functional theory calculation revealed the pyridinic-N facilitated the desorption of NO, which was kinetically involved in the process of the NORR. The findings of this work would be valuable for the development of metal-free NORR electrocatalysts.

Mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) is a clinical syndrome associated with mitochondrial disorders (MIDs). This report illustrates a case of MELAS syndrome with hypothyroidism and psychiatric disorders, which is different from the common clinical manifestations of MELAS syndrome, such as exercise intolerance, migraine-like headaches, hearing loss and seizures etc. There are considerable interests in the possibility that mitochondrial dysfunction may play a role in the pathogenesis of endocrine dysfunctions and psychiatric disorders in MELAS syndrome.

Background: Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthases (NOSs) for the synthesis of nitric oxide (NO). BH4 therapy can reverse the disease-related redox disequilibrium observed with BH4 deficiency. However, whether BH4 exerts a protective effect against radiation-induced damage to cardiomyocytes remains unknown. Methods: Clonogenic assays were performed to determine the effects of X-ray on H9c2 cells with or without BH4 treatment. The contents of lactate dehydrogenase (LDH), superoxide dismutase (SOD), and malondialdehyde (MDA) in H9c2 cells were measured to investigate oxidative stress levels. The cell cycle undergoing radiation with or without BH4 treatment was detected using flow cytometry. The expression levels of proteins in the phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT)/P53 signaling pathway, inducible NOS (iNOS), and endothelial NOS (eNOS) were examined using Western blotting. Results: X-ray radiation significantly inhibited the growth of H9c2 cells in a dose-dependent manner, whereas BH4 treatment significantly reduced the X-ray radiation-induced growth inhibition (control group vs. X-ray groups, respectively, P< 0.01). X-ray radiation induced LDH release, apoptosis, and G0/G1 peak accumulation, significantly increasing the level of MDA and the production of NO, and decreased the level of SOD (control group vs. X-ray groups, respectively, P < 0.05 or P < 0.01). By contrast, BH4 treatment can significantly reverse these processes (BH4 treatment groups vs. X-ray groups, P < 0.05 or P < 0.01). BH4 reversed the X-ray radiation-induced expression alterations of apoptosis-related molecules, including B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein, and caspase-3, and molecules of the PI3K/Akt/P53 signaling pathway. BH4 enhanced the production of NO in 2 Gy and 4 Gy radiated groups by upregulating eNOS protein expression and downregulating iNOS protein expression. Conclusions: BH4 treatment can protect against X-ray-induced cardiomyocyte injury, possibly by recoupling eNOS rather than iNOS. BH4 treatment also decreased oxidative stress in radiated H9c2 cells.

Coal cities are an essential impetus for economic development and urbanization processes in China. However, a series of environmental issues provoked by resource exploitation cause the environmental sustainability of coal cities to face enormous challenges. Therefore, on the basis of the time series data of Yulin City from 1996 to 2017, this paper explores the nexus between socioeconomic development and industrial “three wastes” emissions by adopting the Tapio decoupling model, the environmental Kuznets curve (EKC) hypothesis, and the vector auto-regressive (VAR) model. The results show that Yulin’s economic development remains in an extensive stage and will not decouple from the environmental pollution in a short time. Except for the nexus of industrial solid waste and economic growth, which is an inverted U-shaped, the EKC hypothesis is not valid for industrial wastewater and industrial waste gas. Through the VAR (2) model, the impact of per capita gross domestic product (GDP) on industrial waste emissions is consistent with the results of the EKC hypothesis. Moreover, industrial waste emissions have a positive correlation with the per capita raw coal output, the energy consumption per unit of GDP, and the proportion of secondary industry. Hence, it is necessary to formulate targeted measures from industrial restructuring, industrial chain extension, governance model optimization, and waste comprehensive utilization to realize the environmental sustainability of coal cities.

Measles is caused by measles virus belonging to genus Morbillivirus of the family Paramyxoviridae. Vaccination has played a critical role in controlling measles infection worldwide. However, in the recent years, outbreaks of measles infection still occur in many developing countries. Here, we report an outbreak of measles among healthcare workers and among the 60 measles infected patients 50 were healthcare workers including doctors, nurses, staff, and medics. Fifty-one patients (85%) tested positive for IgM antibodies against the measles virus and 50 patients (83.3%) tested positive for measles virus RNA. Surprisingly, 73.3% of the infected individuals had been previously immunized against measles. Since there is no infection division in our hospital, the fever clinics are located in the Emergency Division. In addition, the fever and rash were not recognized as measles symptoms at the beginning of the outbreak. These factors result in delay in isolation and early confirmation of the suspected patients and eventually a measles outbreak in the hospital. Our report highlights the importance of following a two-dose measles vaccine program in people including the healthcare workers. In addition, vigilant attention should be paid to medical staff with clinical fever and rash symptoms to avoid a possible nosocomial transmission of measles infection.