1. Optimization of Free Phytoprostane and Phytofuran Production by Enzymatic Hydrolysis of Pea Extracts Using Esterases
- Author
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Jean-Marie Galano, Raúl Domínguez-Perles, M. Rodríguez-Hernández, Angel Gil-Izquierdo, Thierry Durand, Camille Oger, Federico Ferreres, Iván López-González, Alexandre Guy, I. Sánchez-Martínez, Universidad de Murcia, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,esterase ,[CHIM.THER]Chemical Sciences/Medicinal Chemistry ,01 natural sciences ,Esterase ,response surface methodology ,Hydrolysis ,plant oxylipins ,Enzymatic hydrolysis ,Response surface methodology ,Furans ,Alkaline hydrolysis ,chemistry.chemical_classification ,Chromatography ,Plant Extracts ,010401 analytical chemistry ,Esterases ,Peas ,enzymatic hydrolysis ,General Chemistry ,Box–Behnken design ,0104 chemical sciences ,Enzyme ,chemistry ,Yield (chemistry) ,free plant oxylipins ,General Agricultural and Biological Sciences ,Box−Behnken ,010606 plant biology & botany - Abstract
International audience; Given the growing interest in phytoprostanes (PhytoPs) and phytofurans (PhytoFs) in the fields of plant physiology, biotechnology, and biological function, the present study aims to optimize a method of enzymatic hydrolysis that utilizes bacterial and yeast esterases that allow the appropriate quantification of PhytoPs and PhytoFs. To obtain the highest concentration of PhytoPs and PhytoFs, a response surface methodology/Box−Behnken design was used to optimize the hydrolysis conditions. Based on the information available in the literature on the most critical parameters that influence the activity of esterases, the three variables selected for the study were temperature (°C), time (min), and enzyme concentration (%). The optimal hydrolysis conditions retrieved differed between PhytoPs (21.5°C, 5.7 min, and 0.61 μg of enzyme per reaction) and PhytoFs (20.0°C, 5.0 min, and 2.17 μg of enzyme per reaction) and provided up to 25.1-and 1.7-fold higher contents relative to nonhydrolyzed extracts. The models were validated by comparing theoretical and experimental values for PhytoP and PhytoF yields (1.01 and 1.06 theoretical/experimental rates, respectively). The optimal conditions were evaluated for their relative influence on the yield of individual nonesterified PhytoPs and PhytoFs to define the limitations of the models for obtaining the highest concentration of most considered compounds. In conclusion, the models developed provided valuable alternatives to the currently applied methods using unspecific alkaline hydrolysis to obtain free nonesterified PhytoPs and PhytoFs, which give rise to more specific hydrolysis of PhytoP and PhytoF esters, reducing the degradation of free compounds by classical chemical procedures.
- Published
- 2020