35 results on '"Carvalho, Denise Pires de"'
Search Results
2. Aggressive nonfunctioning pituitary neuroendocrine tumors
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Portovedo, Sérgio, Neto, Leonardo Vieira, Soares, Paula, Carvalho, Denise Pires de, Takiya, Christina Maeda, and Miranda-Alves, Leandro
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- 2022
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3. Environmentally relevant dose of the endocrine disruptor tributyltin disturbs redox balance in female thyroid gland
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Andrade, Marcelle Novaes, Melo-Paiva, Francisca Diana, Teixeira, Mariana Pires, Lima-Junior, Niedson Correia de, Soares, Paula, Graceli, Jones Bernardes, Carvalho, Denise Pires de, Morris, Eduardo Andrès Rios, Ferreira, Andrea Claudia Freitas, and Miranda-Alves, Leandro
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- 2022
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4. Hydroxyapatite calvaria graft repair in experimental diabetes mellitus in rats
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Barbirato, Davi da Silva, Fogacci, Mariana Fampa, Gusman, Heloisa, Takiya, Christina Maeda, Carvalho, Denise Pires de, and Samsone, Carmelo
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- 2018
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5. Binge eating disorder, frequency of depression, and systemic inflammatory state in individuals with obesity – A cross sectional study
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Caldas, Nelson do Rosário, primary, Braulio, Valeria Bender, additional, Brasil, Marco Antônio Alves, additional, Furtado, Valeria Cristina Soares, additional, Carvalho, Denise Pires de, additional, Cotrik, Ervin Michelstaedter, additional, Dantas, Joana Rodrigues, additional, and Zajdenverg, Lenita, additional
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- 2022
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6. The flavonoid quercetin reduces cell migration and increases NIS and E-cadherin mRNA in the human thyroid cancer cell line BCPAP
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Gonçalves, Carlos Frederico Lima, primary, Hecht, Fabio, additional, Cazarin, Juliana, additional, Fortunato, Rodrigo Soares, additional, Vaisman, Mario, additional, Carvalho, Denise Pires de, additional, and Ferreira, Andrea Claudia Freitas, additional
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- 2021
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7. Functional Consequences of Dual Oxidase-Thyroperoxidase Interaction at the Plasma Membrane
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Fortunato, Rodrigo Soares, Lima de Souza, Elaine Cristina, Hassani, Rabii Ameziane-el, Boufraqech, Myriem, Weyemi, Urbain, Talbot, Monique, Lagente-Chevallier, Odile, Carvalho, Denise Pires de, Bidart, Jean-Michel, Schlumberger, Martin, and Dupuy, Corinne
- Published
- 2010
8. A Compendium of Physical Exercise-related Human Genes: An'OMIC Scale Analysi
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Pacheco, Christina, Felipe, Stela Mirla da Silva, Soares, Milca Magalhães Dias de Carvalho, Alves, Juliana Osório, Soares, Paula Matias, Leal-Cardoso, José Henrique, Loureiro, Adriano César Carneiro, Ferraz, Alex Soares Marreiros, Carvalho, Denise Pires de, and Ceccatto, Vânia Marilande
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Transcriptoma de exercício físico ,Genômica do exercício ,Genes ligados ao exercício físico ,Fisiologia do exercício molecular ,Ontologia genética do exercício - Abstract
Regular exercise is an exogenous factor of gene regulation with numerous health benefits. The study aimed to evaluate human genes linked to physical exercise in an ‘omic scale, addressing biological questions to the generated database. Three literature databases were searched with the terms ‘exercise’, ‘fitness’, ‘physical activity’, ‘genetics’ and ‘gene expression’. For additional references, papers were scrutinized and a textmining tool was used. Papers linking genes to exercise in humans through microarray, RNA-Seq, RT-PCR and genotyping studies were included. Genes were extracted from the collected literature, together with information on exercise protocol, experimental design, gender, age, number of individuals, analytical method, fold change and statistical data. The ‘omic scale dataset was characterized and evaluated with bioinformatics tools searching for gene expression patterns, functional meaning and gene clusters. As a result, a physical exercise-related human gene compendium was created, with data from 58 scientific papers and 5.147 genes functionally correlated with 17 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. While 50.9% of the gene set was up-regulated, 41.9% was down-regulated. 743 up- and 530 down-regulated clusters were found, some connected by regulatory networks. To summarize, up- and down-regulation was encountered, with a wide genomic distribution of the gene set and up- and down-regulated clusters possibly assembled by functional gene evolution. Physical exercise elicits a widespread response in gene expression.
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- 2018
9. Effects of Metformin on TSH Levels and Benign Nodular Goiter Volume in Patients Without Insulin Resistance or Iodine Insufficiency
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dos Santos, Patricia Borges, primary, Gertrudes, Larissa Nascimento, additional, Conceição, Flavia Lucia, additional, de Andrade, Bruno Moulin, additional, Carvalho, Denise Pires de, additional, Vaisman, Mario, additional, and Teixeira, Patricia de Fatima dos Santos, additional
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- 2019
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10. Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome
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Pauer, Heidi, primary, Hardoim, Cristiane Cassiolato Pires, additional, Teixeira, Felipe Lopes, additional, Miranda, Karla Rodrigues, additional, Barbirato, Davi da Silva, additional, Carvalho, Denise Pires de, additional, Antunes, Luis Caetano Martha, additional, Leitão, Álvaro Augusto da Costa, additional, Lobo, Leandro Araujo, additional, and Domingues, Regina Maria Cavalcanti Pilotto, additional
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- 2018
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11. HMGA2 as new biomarker of pituitary adenomas invasiveness?
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Christina Maeda Takiya, Leandro Miranda-Alves, Paula Matias Soares, Nathalie Henriques Canedo, Santos Faria Manuel dos, Sergio Filipe Portovedo, Carvalho Denise Pires de, Bruno de Almeida Nunes, Gilvan Cortês Nascimento, and Fernanda Oliveira Graca
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HMGA2 ,biology ,business.industry ,Cancer research ,biology.protein ,Medicine ,Biomarker (medicine) ,business - Published
- 2016
12. A compendium of physical exercise-related human genes: an ’omic scale analysis
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Pacheco, Christina, primary, Felipe, Stela Mirla da Silva, additional, Soares, Milca Magalhães Dias de Carvalho, additional, Alves, Juliana Osório, additional, Soares, Paula Matias, additional, Leal-Cardoso, José Henrique, additional, Loureiro, Adriano César Carneiro, additional, Ferraz, Alex Soares Marreiros, additional, Carvalho, Denise Pires de, additional, and Ceccatto, Vânia Marilande, additional
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- 2017
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13. Zumbido em uma população ribeirinha exposta ao metilmercúrio
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Cardoso, Nathalia Alves, Hoshino, Ana Cristina Hiromi, Perez, Maurício Andrade, Bastos, Wanderley Rodrigues, Carvalho, Denise Pires de, and Câmara, Volney de Magalhães
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Questionnaires ,Tinnitus ,Public health ,Zumbido ,Saúde pública ,Questionários ,Mercury ,Hearing loss ,Perda auditiva ,Mercúrio - Abstract
Objetivo : Estudar a prevalência de zumbido, verificando se há associação entre a queixa desse sintoma e o teor de mercúrio e mensurar seu impacto na qualidade de vida. Métodos : Estudo seccional em toda a população do Lago do Puruzinho, localizada na margem esquerda do Rio Madeira, cidade de Humaitá, Estado do Amazonas, Brasil. Todos os residentes maiores de 18 anos, de ambos os gêneros, foram submetidos à anamnese, avaliação otorrinolaringológica e exame audiológico. Os indivíduos que apresentaram queixa de zumbido responderam à versão traduzida do questionário Tinnitus Handicap Inventory (THI). Também foram pesquisados os teores de mercúrio total no cabelo desses indivíduos. Resultados: Para análise dos resultados sobre a queixa de zumbido, os sujeitos foram divididos em dois grupos quanto à presença ou não de zumbido. O Grupo 1 foi composto por indivíduos que apresentaram queixa de zumbido e o Grupo 2, por indivíduos sem zumbido. Foi observado que 40% dos indivíduos apresentaram escores do THI compatíveis com handicap leve. A análise da associação da presença de zumbido com os teores de mercúrio total no cabelo mostrou que ambos os grupos apresentaram níveis elevados de mercúrio, porém não ocorreram diferenças entre os grupos. Conclusão : Um quarto dos ribeirinhos apresentou queixa de zumbido e exposição significativa ao mercúrio, mas não houve associação entre o zumbido e os níveis elevados de mercúrio. Purpose : To study the association between the prevalence of tinnitus and mercury exposure and measure the influence of tinnitus on the quality of life. Methods : We conducted a cross-sectional study of men and women aged above 18 years residing in the Lake Puruzinho region, which is located on the left bank of the Rio Madeira, Humaita city, Amazonas state, Brazil. All subjects underwent anamnesis, otorhinolaryngologic evaluation, and an audiology test. Individuals who experienced tinnitus completed the translated Tinnitus Handicap Inventory (THI). We also examined the levels of total mercury in the hair of these individuals. Results : To analyze the results regarding the prevalence of tinnitus, the subjects were divided into two groups according to the presence or absence of tinnitus. Group 1 was composed of subjects with tinnitus and Group 2 was composed of subjects without tinnitus. Consequently, 40% of individuals had scores consistent with mild handicap according to the THI. The analysis of the association between tinnitus and levels of total mercury in hair samples showed that both groups had high levels of mercury. However, this finding was not significantly different between groups. Conclusion : Herein, 25% of the subjects complained of tinnitus and significant exposure to mercury; however, there was no association between the prevalence of tinnitus and high levels of mercury.
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- 2014
14. Expression of connexins 26, 32 and 43 mRNA in normal and pituitary adenomas
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Urlich Renner, Santos Faria Manuel dos, Carvalho Denise Pires de, Bruno de Almeida Nunes, Rodrigo S. Fortunato, Gunter K. Stalla, Leandro Miranda-Alves, and Nasciutti Luiz Eurico
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Messenger RNA ,Expression (architecture) ,Biology ,Molecular biology - Published
- 2013
15. Repercussão clínica da reclassificação dos carcinomas diferenciados de tireóide de acordo com a 6ª edição do TNM
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Varandas, Vanessa M., Coelho, Sabrina Mendes, Soeiro, Ana Paula V., Coeli, Cláudia Medina, Carvalho, Denise Pires de, Buescu, Alexandru, and Vaisman, Mário
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Staging ,Prognóstico ,Estadiamento ,Câncer de tireóide ,Prognosis ,Thyroid cancer - Abstract
A classificação TNM segundo a UICC é usada para avaliar os resultados do acompanhamento do carcinoma de tireóide. A 6ª edição modificou a descrição do tumor primário (T), dos linfonodos regionais (N) e dos grupos de estadiamento. O objetivo deste estudo foi comparar a habilidade das 5ª e 6ª edições em predizer resultados. As duas classificações foram aplicadas em uma análise retrospectiva de 90 pacientes do HUCFF. Sessenta e nove pacientes apresentavam carcinoma papilífero, 14 folicular, 4 células de Hürthle e 3 misto. Os pacientes foram acompanhados por um período médio de 58,3 meses. Ao final do acompanhamento, 49 pacientes estavam em remissão, 23 com doença persistente, 4 com recorrência tumoral, 11 com metástases e 3 evoluíram para o óbito. De acordo com a 6ª edição, 19 pacientes foram classificados como T1, comparado com 7 pela 5ª edição; 19 pacientes T2 comparado com 30; 14 classificados como T3 comparado com 10; 22 como T4 comparado com 27, e 16 pacientes como Tx. Ambas as edições mostraram remissões comparáveis para os estágios I, II e III. Para o estágio IV houve uma mudança significativa na remissão, entretanto não houve diferença comparando IV e IV C. The TNM classification of UICC is used for predicting the outcome of thyroid cancer. The 6th edition changed the description of primary tumor (T), regional lymph node (N) and the staging group. The aim of this study was to compare the ability of the 5th and the 6th editions to predict outcome. The two classifications were applied in a retrospective analysis of 90 patients from HUCFF. Sixty-nine patients had papillary carcinoma, 14 follicular, 4 Hürthle cell, and 3 mixed. Patients were followed for a mean period of 58.3 months. At the end of follow-up, 49 patients were disease-free, 23 persisted with disease, 4 had cervical recurrence, 11 had metastases and 3 died. According to the 6th edition, 19 patients were classified as T1, compared to 7 based on the 5th edition; 19 patients were T2 compared to 30; 14 were T3 compared to 10; 22 were T4 compared to 27, and 16 patients were Tx. Both editions showed comparable remissions for stages I, II, and III. For the stage IV there was a significant change in remission, however there was no difference comparing IV and IV C.
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- 2007
16. New perspectives on the treatment of differentiated thyroid cancer
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Coelho, Sabrina Mendes, Carvalho, Denise Pires de, and Vaisman, Mário
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Targeted therapy ,Tyrosine kinase inhibitors ,Câncer da tireóide ,Redifferentiation therapy ,Inibidor de tirosina cinase ,Rediferenciação tumoral ,Terapia direcionada ,Thyroid cancer - Abstract
Even though differentiated thyroid carcinoma is a slow growing and usually curable disease, recurrence occurs in 20-40% and cellular dedifferentiation in up to 5% of cases. Conventional chemotherapy and radiotherapy have just a modest effect on advanced thyroid cancer. Therefore, dedifferentiated thyroid cancer represents a therapeutic dilemma and a critical area of research. Targeted therapy, a new generation of anticancer treatment, is planned to interfere with a specific molecular target, typically a protein that is believed to have a critical role in tumor growth or progression. Since many of the tumor-initiation events have already been identified in thyroid carcinogenesis, targeted therapy is a promising therapeutic tool for advanced thyroid cancer. Several new drugs are currently being tested in in vitro and in vivo studies and some of them are already being used in clinical trials, like small molecule tyrosine kinase inhibitors. In this review, we discuss the bases of targeted therapies, the principal drugs already tested and also options of redifferentiation therapy for thyroid carcinoma. Apesar de o carcinoma diferenciado da tireóide ser considerado uma doença de curso indolente e geralmente curável, recorrência tumoral ocorre em aproximadamente 20 a 40% e desdiferenciação celular, em até 5% dos casos. A quimioterapia convencional e a radioterapia apresentam apenas um modesto efeito sobre o câncer de tireóide avançado. Dessa forma, o carcinoma da tireóide desdiferenciado representa um dilema terapêutico e uma importante área de pesquisa. A terapia direcionada, uma nova geração de tratamento para o câncer, tem como objetivo interferir com um alvo molecular específico, geralmente uma proteína considerada fundamental para o crescimento e progressão tumoral. Como muitos eventos iniciadores do processo de carcinogênese tireoideana já foram identificados, a terapia direcionada representa uma promissora opção terapêutica para o carcinoma da tireóide avançado. Várias drogas novas estão em estudos in vitro e in vivo e algumas já estão sendo testadas em estudos clínicos, como as pequenas moléculas inibidoras de tirosina cinase. Nesta revisão, as bases moleculares da terapia direcionada, as principais drogas utilizadas e as opções terapêuticas de rediferenciação do carcinoma da tireóide serão discutidas.
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- 2007
17. Zumbido em uma população ribeirinha exposta ao metilmercúrio
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Cardoso, Nathalia Alves, primary, Hoshino, Ana Cristina Hiromi, additional, Perez, Maurício Andrade, additional, Bastos, Wanderley Rodrigues, additional, Carvalho, Denise Pires de, additional, and Câmara, Volney de Magalhães, additional
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- 2014
- Full Text
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18. Correction: Flavonoid Rutin Increases Thyroid Iodide Uptake in Rats
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Gonçalves, Carlos Frederico Lima, primary, Santos, Maria Carolina de Souza dos, additional, Ginabreda, Maria Gloria, additional, Fortunato, Rodrigo Soares, additional, Carvalho, Denise Pires de, additional, and Freitas Ferreira, Andrea Claudia, additional
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- 2014
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19. Tributyltin changes the thyroid gland morphology of male rats
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Pereira, Paula Rodrigues, primary, Mattos, Romulo Medina de, additional, Palmero, Celia Yelimar, additional, Fortunato, Rodrigo, additional, Gracelli, Jones Bernardes, additional, Carvalho, Denise Pires de, additional, Nasciutti, Luiz Eurico, additional, and Miranda-Alves, Leandro, additional
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- 2013
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20. AMP-ACTIVATED PROTEIN KINASE UP REGULATES GLUCOSE UPTAKE IN THYROID PCCL3 CELLS INDEPENDENT OF THYROTROPIN
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Andrade, Bruno Moulin, primary, Cazarin, Juliana Menezes, additional, Zancan, Patricia, additional, and Carvalho, Denise Pires de, additional
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- 2012
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21. The mTOR protein as a target in thyroid cancer
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Souza, Elaine Cristina Lima de, primary, Ferreira, Andrea Claudia Freitas, additional, and Carvalho, Denise Pires de, additional
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- 2011
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22. MTOR downregulates iodide uptake in thyrocytes
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Souza, Elaine Cristina Lima de, primary, Padrón, Álvaro Souto, additional, Braga, William Miranda Oliveira, additional, Andrade, Bruno Moulin de, additional, Vaisman, Mário, additional, Nasciutti, Luiz Eurico, additional, Ferreira, Andrea Claudia Freitas, additional, and Carvalho, Denise Pires de, additional
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- 2010
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23. Gestão das inovações incrementais, o caso Omeprazola
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Lourenço, Alexandre Lopes, primary, Lira, Luiz Marcelo, additional, Carvalho, Denise Pires de, additional, Melo, Paulo de Assis, additional, and Cláudio-da-Silva, Tereza Sollero, additional
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- 2010
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24. Repercussão clínica da reclassificação dos carcinomas diferenciados de tireóide de acordo com a 6ª edição do TNM
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Varandas, Vanessa M., primary, Coelho, Sabrina Mendes, additional, Soeiro, Ana Paula V., additional, Coeli, Cláudia Medina, additional, Carvalho, Denise Pires de, additional, Buescu, Alexandru, additional, and Vaisman, Mário, additional
- Published
- 2007
- Full Text
- View/download PDF
25. New perspectives on the treatment of differentiated thyroid cancer
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Coelho, Sabrina Mendes, primary, Carvalho, Denise Pires de, additional, and Vaisman, Mário, additional
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- 2007
- Full Text
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26. Amplificação de mRNA de tireoglobulina no sangue de pacientes com carcinoma diferenciado da tireóide: qual o seu verdadeiro significado?
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Coelho, Sabrina Mendes, primary, Vaisman, Mário, additional, and Carvalho, Denise Pires de, additional
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- 2006
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27. Effect of Retinoic Acid on Metastatic Follicular Thyroid Carcinoma
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Coelho, Sabrina Mendes, Figueiredo, Marcia Denise Lima de, Corbo, Rossana, Carvalho, Denise Pires de, and Vaisman, Mario
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The overall prognosis of differentiated thyroid cancer (DTC) is favorable, although patients with metastasized undifferentiated carcinoma have a less favorable prognosis. These metastasis usually are resistant to conventional radio- or chemotherapy. Thus, the search for new therapeutic options is important. Experimental and clinical studies show that retinoic acid (RA) has anti-proliferative effects and can induce thyroid follicular cell redifferentiation. Based on these data, we administered 13-cis-RA to a patient with follicular thyroid cancer, persistent detectable thyroglobulin and pulmonary metastases, who could not concentrate radioiodide after cumulative dose of 200mCi of 131iodide (131I). Chemotherapy was the first therapeutic choice, but no significant response was observed. After 5 weeks of treatment with 13-cis-RA, the pulmonary metastasis were able to concentrate radioiodide after a therapeutic dose of 150mCi 131I.
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- 2003
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28. O Knockout para o receptor 1 de TNF-a protege contra obesidade induzida por dieta
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Romanatto, Talita, Velloso, Licio Augusto, 1963, Carvalho, Denise Pires de, Curi, Tania Cristina Pithon, Saad, Mario José Abdalla, Anhê, Gabriel Forato, Universidade Estadual de Campinas. Faculdade de Ciências Médicas, Programa de Pós-Graduação em Fisiopatologia Médica, and UNIVERSIDADE ESTADUAL DE CAMPINAS
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Inflammation ,Inflamação ,Obesidade ,Tumor necrosis factor alpha ,High fat diet ,Fator de necrose tumoral alfa ,Termogênese ,Thermogenesis ,Obesity ,Dieta hiperlipídica - Abstract
Orientador: Licio Augusto Velloso Tese (doutorado) - Universidade Estadual de Campinas. Faculdade de Ciencias Medicas Resumo: O aumento da prevalência de obesidade em várias regiões do planeta é um dos mais importantes fenômenos clínico-epidemiológicos da atualidade. Fatores como a mudança do hábito alimentar e o estilo de vida sedentário, aliados a determinantes genéticos ainda pouco conhecidos desempenham um papel relevante na patogênese dessa doença. Nos últimos quinze anos, desde o descobrimento do hormônio leptina, avanços consideráveis foram obtidos na caracterização dos mecanismos hipotalâmicos do controle da ingestão alimentar e da termogênese. Em um estudo recente demonstrou-se que o consumo de uma dieta rica, em gordura induz a expressão de várias citocinas pró-inflamatórias no hipotálamo e que a inibição da via de sinalização intracelular da serina quinase JNK é capaz de reverter parcialmente algumas das conseqüências clínicas do consumo dessa dieta (De Souza et al, 2005). No presente estudo avaliou-se a importância do receptor 1 de TNF-cc(TNFRI) na gênese do processo inflamatório desencadeado pela dieta rica em gordura. O TNFR1 é responsável pela maioria dos efeitos do TNF-ct, no entanto no contexto da obesidade induzida por dieta, a função desse receptor não está completamente esclarecida. Para tanto, camundongos knockout (KO) para o TNFR1 e seu respectivo background genético, C57BL6, foram tratados por 8 semanas com dieta hiperlipídica e observamos que o TNFR1 KO está protegido da obesidade induzida por dieta por meio de aumento na termogênese. Em ambas as dietas, padrão e hiperlipídica (HF), TNFR1 KO ganha menos peso apesar de aumento na ingestão alimentar. Adiposidade e diâmetro dos adipócitos estão reduzidos, assim como as concentrações sanguíneas de insulina e leptina. TNFR1 KO estão protegidos de resistência hipotalâmica à via da leptina por meio de preservação da sinalização da leptina através de JAK2, STAT3 e FOX01. TNFR1 KO apresentam aumento de termogênese, pelo aumento do consumo de 02, aumento da expressão de UCP-1 e UCP-3, no tecido adiposo marrom e músculo esquelético, respectivamente, e aumento do consumo de O2 de mitocôndrias isoladas de músculo. Isso demonstra que a via de sinalização do TNF-a pelo TNFR1 representa um importante mecanismo envolvido na termogênese deficiente associada à obesidade. Abstract: In diet-induced obesity, hypothalamic and systemic inflammatory factors trigger intracellular mechanisms that lead to resistance to the main adipostatic hormones, leptin and insulin. Tumor necrosis factor-alpha (TNF-a) is one of the main inflammatory factors produced during this process and its mechanistic role as an inducer of leptin and insulin resistance has been widely investigated. Most of TNF-a inflammatory signals are delivered by TNFR1; however, the role played by this receptor in the context of obesity-associated inflammation is not completely known. Here, we show that TNFR1 knockout (TNFR1 KO) mice are protected from diet-induced obesity due to increased thermogenesis. Under standard rodent chow or ligh-fat diet, TNFR1 KO gain significantly less body mass in spite of increased caloric intake. Visceral adiposity and mean adipocyte diameter are reduced and Dlood concentrations of insulin and leptin are lower. Protection from hypothalamic eptin resistance is evidenced by increased leptin-induced suppression of food ntake and preserved activation of leptin signal transduction through JAK2, STAT3 jnd FOX01. Under high fat diet, TNFR1 KO mice present a significantly increased expression of the thermogenesis-related neurotransmitter, TRH. Further evidence if increased thermogenesis includes the increased 02 consumption and C02 traduction in respirometry measurements, increased expressions of UCP1 and JCP3 in brown adipose tissue and skeletal muscle, respectively, and increased 02 onsumption by isolated skeletal muscle fiber mitochondria. This demonstrates that 'NF-ct signaling through TNFR1 is an important mechanism involved in obesity-ssociated defective thermogenesis. Doutorado Medicina Experimental Doutor em Fisiopatologia Médica
- Published
- 2021
29. Effects of low-dose transdermal estradiol on inflammatory biomarkers and microcirculatory function in overweight and grade I obesity women at post menopause
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Silva, Lucia Henriques Alves da, Aguiar, Luiz Guilherme Kraemer de, Rodacki, Melanie, Carvalho, Denise Pires de, and Souza, Maria das Graças Coelho de
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Inflammation ,Inflamação ,Pós-menopausa Efeito das drogas ,Obesidade ,Estradiol ,Microcirculation ,Menopausa Hormonioterapia ,CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::ENDOCRINOLOGIA [CNPQ] ,Endothelium ,Microcirculação ,Endotélio ,Hormonal menopause therapy ,Terapia hormonal da menopausa - Abstract
Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:58:33Z No. of bitstreams: 1 DISSERTACAO_FINAL_PUBLICADA_Lucia_Henriques_Alves_da_Silva.pdf: 2785932 bytes, checksum: ecf06825ccb0732e772fb516b3543e49 (MD5) Made available in DSpace on 2021-01-06T20:58:33Z (GMT). No. of bitstreams: 1 DISSERTACAO_FINAL_PUBLICADA_Lucia_Henriques_Alves_da_Silva.pdf: 2785932 bytes, checksum: ecf06825ccb0732e772fb516b3543e49 (MD5) Previous issue date: 2014-03-18 Hormonal therapy (HT) is a modality of treatment to postmenopausal women with climacteric syndrome. Many positive effects beyond improvement on related signals and symptoms had been proven, as those observed in bone health and endothelial function. However, there are still doubts about which profile of patients would benefit after physiological plasmatic concentrations of estrogens are achieved, since HT was associated to cardiovascular undesirable events, especially in women with established atherosclerotic disease. The objective of this work was to evaluate the effect of the use of hormonal therapy with low-dose isolated transdermal estradiol in postmenopausal women with overweight and grade I obesity on inflammation biomarkers and microcirculatory function. 44 women who presented recent menopause, aged between 47 a 55 years and body mass index of 27,7 to 34,9 kg∕m² were recruited. They were allocated, by external selection, into two groups: placebo (P) and transdermal estradiol (E). In this group, the volunteers used 1 mg of daily transdermal estradiol during three consecutive months. Plasma levels of inflammatory biomarkers (high sensitive reactive C protein, interleukin-1β, interleukin-6, monocyte chemotactic protein-1 and tumoral necrosis factor-α), vascular injury and repair biomarkers (activated and resting circulating endothelial cells and circulating progenitor cells) were quantified and microcirculatory function analyzed by laser Doppler flowmetry (LDF) before and after intervention. The patients had age of 51,77 ± 2,3 years, BMI of 31,52 ± 2,54 kg/m² and time since menopause of 3 [2-5] years. After intervention, we did not notice changes on inflammatory biomarkers in both groups. However, on the E group, a significant increase on levels of resting circulating endothelial cells and endothelial progenitor cells were observed. In addition, there was a trend toward improvement on endothelial component of vasomotricity (P < 0,057). Moreover, a lower cumulative dose to achieve the plateau maximal velocity after acetylcholine iontophoresis was necessary (P < 0,001) and an improvement on endothelial-independent vasodilation after sodium nitroprusside iontophoresis (P < 0,01) occurred. We did not observe changes in body composition or insulin sensitivity. In conclusion, in postmenopausal women studied, the proposed hormonal therapy did not modify plasma inflammatory biomarkers, but resulted in benefits on markers of vascular repair and microcirculatory reactivity. A terapia hormonal (TH) é uma modalidade de tratamento para mulheres na pós-menopausa com a síndrome do climatério. Inúmeros efeitos positivos além da melhora dos sinais e sintomas relacionados já foram demonstrados, como aqueles observados na saúde óssea e na função endotelial. Porém, ainda há dúvidas sobre que perfis de pacientes mais se beneficiariam do restabelecimento de concentrações plasmáticas de estrogênios ditas fisiológicas, uma vez que a TH foi associada a eventos cardiovasculares desfavoráveis, especialmente em pacientes com doença ateroesclerótica estabelecida. O objetivo deste trabalho foi avaliar o efeito do uso de terapia hormonal com estradiol transdérmico em monoterapia e em baixa dose em mulheres com sobrepeso ou obesidade na pós-menopausa sobre biomarcadores de inflamação e sobre a função microcirculatória. Foram recrutadas 44 mulheres na pós-menopausa recente com idade entre 47 a 55 anos e índice de massa corporal (IMC) de 27,5 a 34,9 kg/m² e posteriormente alocadas, por seleção externa, em dois grupos: placebo (P) e estradiol transdérmico (E). Neste grupo, as voluntárias fizeram uso de estradiol hemi-hidratado transdérmico na dose de 1 mg diários, por um período de três meses consecutivos. Foram realizadas medidas plasmáticas de biomarcadores de inflamação (proteína C reativa ultrassensível, interleucina 1β, interleucina 6, proteína quimiotática de monócitos-1 e fator de necrose tumoral-α), biomarcadores de lesão e reparo vascular (células endoteliais circulantes ativadas e em repouso e células endoteliais progenitoras) e avaliação de função microcirculatória por fluxometria por laser Doppler (LDF) antes e após a intervenção. As pacientes apresentavam idade de 51,77 ± 2,3 anos, IMC de 31,52 ± 2,54 kg/m² e tempo de menopausa de 3 [2-5] anos. Após a intervenção não houve mudanças nas concentrações plasmáticas dos biomarcadores de inflamação em ambos os grupos; entretanto, no grupo E, houve um aumento significativo no número de células endoteliais circulantes em repouso e das células endoteliais progenitoras (P < 0,01). Além disso, houve uma tendência à mudança no componente endotelial de vasomotricidade (P = 0,057), além de menor dose cumulativa necessária para alcance do plateau da velocidade máxima após a iontoforese com acetilcolina (P < 0,001) e houve melhora da vasodilatação endotélio-independente após iontoforese com nitroprussiato de sódio (P < 0,01). Não houve qualquer mudança em composição corporal ou sensibilidade à ação da insulina na população estudada. Em conclusão, em mulheres na pós-menopausa com o perfil estudado, a terapia hormonal proposta não interferiu sobre biomarcadores inflamatórios plasmáticos, mas promoveu ações benéficas em biomarcadores de reparo vascular e reatividade microcirculatória.
- Published
- 2014
30. Evaluation of the effect of fat overload in microvascular reactivity in obese women
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Maranhão, Priscila Alves, Bouskela, Eliete, Aguiar, Luiz Guilherme Kraemer de, Tibiriçá, Eduardo Vera, Carvalho, Denise Pires de, Sanjuliani, Antonio Felipe, and Moura, Egberto Gaspar de
- Subjects
Microcirculation dysfunction ,Postprandial lipemia ,Obesidade nas mulheres ,Doenças cardiovasculares Fatores de risco ,Videocapilaroscopia ,Lipídios Distúrbios do metabolismo ,Hiperlipidemia ,Obesidade ,Disfunção microcirculatória ,Lipemia pós-prandial ,Obesity ,Período pós-prandial ,Mulheres Hábitos alimentares ,CIENCIAS DA SAUDE::NUTRICAO [CNPQ] ,Videocapillaroscopy - Abstract
Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:53:12Z No. of bitstreams: 1 Tese_Final_Priscila Alves Maranhao.pdf: 1258682 bytes, checksum: 84ec9b1ac404b329ff94e92fe884e536 (MD5) Made available in DSpace on 2021-01-06T20:53:12Z (GMT). No. of bitstreams: 1 Tese_Final_Priscila Alves Maranhao.pdf: 1258682 bytes, checksum: 84ec9b1ac404b329ff94e92fe884e536 (MD5) Previous issue date: 2013-04-24 Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro Changes in eating habits have caused striking effects on public health, directly related to increased intake of food rich in fat, mainly saturated fat. The main consequence of this consumption is the excessive and prolonged state of postprandial lipemia (PPL), considered one an important factor related to metabolic abnormalities and vascular damage. The aim of this study was to assess effects of fat overload on microvascular reactivity in obese women. Of the 41 study participants, 21 had the diagnosis of obesity, with BMI of 32.4 ± 1.6 kg/m2 (mean ± SD) and age of 31.6 ± 5 years and 20 healthy women with BMI of 21.9 ± 1.7 kg/m2and age 27.2 ± 5.5 years. After clinical and laboratorial assessment, participants had the microcirculation examined by two methods: dynamic, using the nailfold bed to assess functional capillary density (FCD), red blood cell velocity in in control conditions (RBCV) and peak (RBCVmax) and time (TRBCVmax) to reach it after 1 min arterial occlusion. The second technique was the finger dorsum to assess FCD at rest and during the reactive hyperemia response and after venous occlusion. Blood sampling was performed to determine total cholesterol (TC), triglycerides (TG), HDL- c and free fatty acids (FFA), glucose, insulin and plasma viscosity at 30 and 50 rotations per minute (rpm). Systolic (SBP) and diastolic (DBP) blood pressures and heart rate (HR) were also measured. After these measurements at rest, all participants received a meal rich in lipids, and after 30, 60, 120 and 180 min after ingestion, videocapillaroscopy exams and blood samples were taken again. Results - Obese participants, after fat overload, presented significantly lower values than at rest at finger dorsum of FCD (p = 0.02), FCD during reactive hyperemia (p = 0.02) and post- venous occlusion (p = 0.02), HDL-C (p
- Published
- 2013
31. Genic characterizationof monocytes subpopulations CD14+CD16-, CD14+CD16+ e CD14dimCD16+ during obesity
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Martins, Mariana Renovato, Fidalgo, Thereza Christina Barja, Silva, Simone Vargas da, Bouskela, Eliete, Carvalho, Denise Pires de, Cunha, Fernando de Queiroz, and Bozza, Marcelo Torres
- Subjects
Subpopulações de monócitos ,Monocytes subpopulations ,CX3CR1 ,Gastric bypass ,Obesidade ,Cirurgia bariátrica ,Monócitos ,CIENCIAS BIOLOGICAS::FARMACOLOGIA::FARMACOLOGIA BIOQUIMICA E MOLECULAR [CNPQ] ,Antígenos CD14 ,Obesity ,Expressão gênica ,TLR8 - Abstract
Submitted by Boris INFORMAT (boris@uerj.br) on 2021-04-26T01:11:45Z No. of bitstreams: 1 Mariana Renovato Martins Tese completa.pdf: 4410821 bytes, checksum: 4ad24b517758c8557c465509e40d101f (MD5) Made available in DSpace on 2021-04-26T01:11:45Z (GMT). No. of bitstreams: 1 Mariana Renovato Martins Tese completa.pdf: 4410821 bytes, checksum: 4ad24b517758c8557c465509e40d101f (MD5) Previous issue date: 2012-02-29 Conselho Nacional de Desenvolvimento Científico e Tecnológico Obesity is a low-grade inflammatory state characterized by excessive accumulation of adipose tissue which contributes to inflammatory process. Adipose tissue macrophages (ATMs) secret different factors and are polarized into pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes. Most of the ATMs derive from circulating monocytes, so monocytes play an important role in this low-grade inflammatory condition. In recent years, three subsets of monocytes have been identified: The CD14+CD16- (CMI), the CD14+CD16+ (IM) and the CD14dim CD16+ (NCM). Studies have shown an increase in CD16+ (CD14+CD16+ and CD14dimCD16+) subset in human inflammatory chronic pathologies. Recently, it was demonstrated an increase in CD16+ subsets in obesity decreasing after weight loss. Here, we show a set of data of the increased percentages and numbers of IM and NCM in obese subjects that decreased with weight loss (Poitou et al. 2011), and characterize gene expression profile of the monocyte subpopulations in obesity and suggest for the first time that the secretion derived from the obese adipose tissue induces the differentiation of CM into CD16+ monocytes. Furthermore we characterize the frequencies of the three monocytes subpopulations in obese and overweight children. The CM, IM and NCM of 6 obese subjects (OB) before and three-month after the gastric bypass, 6 obese subjects with type II diabetes (OB/D) and 7 lean subjects (C) were sorted by flow cytometry. The expression of several genes involved in monocyte functions (migration, adhesion, phagocytosis and cytokines production) was analyzed by Low Density Array. We observed an increase in the frequency of CD16+ monocytes in obese and diabetic obese subjects which decreased three months after the weight loss. The gene expression of CX3CR1 (fractalkine receptor) and of TLR8 (Toll Like Receptor 8) was highly increased in the CM, IM and NCM of the OB group and decreased after surgery. The three subsets displayed different gene expression profiles in the OB group compared to the C group: the NCM expressed high levels of CSF1R (Colony Stimulating Factor 1 receptor), TLR4, SELPLG (selectin P ligand) TNFα and IL1, the IM were characterized by over-expression of CCR2, CX3CR1, CCR5, TNFα and MCP1 and the CM expressed high levels of CCR2 (C-C chemokine receptor type 2) and CD36. Our results showed that CD14+CD16- monocytes can differentiate into CD16+ monocytes when stimulated with the conditioning media of visceral adipose tissue, suggesting a pivotal role for adipose tissue in the increased frequency of these monocytes during obesity. The three subpopulations display different gene expression patterns of molecules involved in migration, inflammation and antibody capture. CX3CR1 and TLR8 could be considered as a molecular signature reflecting modified functions of monocytes in obesity. A obesidade é definida como um acúmulo de gordura normal ou excessivo capaz de comprometer a saúde. Sabe-se que a mesma é uma condição inflamatória de baixo grau caracterizada pelo acúmulo excessivo de tecido adiposo, o que contribui para o processo inflamatório. Nesse contexto os macrófagos do tecido adiposo (ATM) secretam diversos fatores e são polarizados nos fenótipos pró-inflamatório (M1) e anti-inflamatório (M2). A maioria dos ATMs deriva de monócitos circulantes e desta forma destaca-se o importante papel dos monócitos nesse quadro de inflamação de baixo-grau. Recentemente, três subpopulações de monócitos foram identificadas: Os CD14+CD16- (MC), os CD14+CD16+ (MI) e os CD14dim CD16+ (MNC). Estudos têm demonstrado um aumento nos monócitos CD16+ (MNC e MI) em doenças inflamatórias em humanos. Recentemente, foi demonstrado que o aumento nos monócitos CD16+ também acontece na obesidade e diminui após a perda de peso. No presente trabalho apresentamos uma parte dos dados que demonstraram que as frequências dos monócitos MI e MNC estão aumentadas em obesos e diminuem com a perda de peso (Poitou e col, 2011). Além disso, caracterizamos o perfil de expressão gênica das subpopulações de monócitos na obesidade e sugerimos, pela primeira vez, que o meio condicionado do adiposo induz a diferenciação dos monócitos clássicos em monócitos CD16+. Ademais caracterizamos a frequência das três subpopulações de monócitos em crianças obesas e com sobrepeso. Os monócitos MC, MI e MNC de 6 indivíduos obesos (OB) antes e 3 meses após o by-pass gástrico (OB/AC), 6 indivíduos com diabetes do tipo II e 7 indivíduos eutróficos foram purificados por citometria de fluxo. As expressões de diversos genes envolvidos nos processos de migração, adesão, fagocitose e produção de citocinas e quimiocinas foram analisadas através da técnica de microarranjo. Observamos um aumento na frequência dos monócitos CD16+ nos indivíduos adultos obesos e obesos com diabetes, os quais estavam diminuídos três meses após a perda de peso. As expressões gênicas de CX3CR1 e de TLR8 estavam aumentadas nos monócitos MC, MI e MNC do grupo OB e diminuíram após a cirurgia. As três subpopulações de monócitos apresentaram perfis de expressão gênica diferentes no grupo OB comparado com o grupo C: os monócitos MNC expressaram níveis elevados de CSF1R, SELPLG, TLR4, TNFα e IL1β, os monócitos MI se caracterizaram pela expressão aumentada de CCR2, CX3CR1, CCR5 TNFα e MCP1 e os monócitos CD14+CD16- apresentaram níveis elevados de CCR2, CX3CR1, CD36 e TLR4. Nossos resultados demonstraram que os monócitos CD14+CD16- podem se diferenciar em monócitos CD16+ quando estimulados com o meio condicionado do tecido adiposo visceral, sugerindo uma função primordial do tecido adiposo no aumento da frequência desses monócitos durante a obesidade. As três subpopulações apresentam padrões diferentes de expressão gênica de moléculas envolvidas na migração, adesão e inflamação. Nesse contexto CX3CR1 e TLR8 podem ser considerados como uma assinatura molecular refletindo as funções monocitárias alteradas na obesidade.
- Published
- 2012
32. Relationships among cephalic hase of digestion, food intake and microvascular reactivity in healthy subjects
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Buss, Caroline, Aguiar, Luiz Guilherme Kraemer de, Bouskela, Eliete, Schaan, Beatriz D'agord, Carvalho, Denise Pires de, Moura, Egberto Gaspar de, and Bordallo, Maria Alice Neves
- Subjects
Consumo alimentar ,Cephalic phase of digestion ,Food intake ,Microcirculation ,Insulina ,Insulin ,Fase cefálica da digestão ,Reatividade microvascular ,Microcirculação ,CIENCIAS BIOLOGICAS::FISIOLOGIA [CNPQ] ,Microvascular reactivity - Abstract
Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:52:30Z No. of bitstreams: 1 Caroline Buss Tese completa.pdf: 1149230 bytes, checksum: b33c8ce5180c660c3eda1cc01fda2fb9 (MD5) Made available in DSpace on 2021-01-06T20:52:30Z (GMT). No. of bitstreams: 1 Caroline Buss Tese completa.pdf: 1149230 bytes, checksum: b33c8ce5180c660c3eda1cc01fda2fb9 (MD5) Previous issue date: 2010-12-13 Conselho Nacional de Desenvolvimento Científico e Tecnológico The cephalic phase of insulin secretion (CPIS) occurs within the cephalic phase of digestion, in approximately ten minutes between the oral-sensorial stimulation elicited by the food to be consumed and the beginning of its absorption. It determines a rapid increase in insulin levels. The CPIS is believed to be important for normal glucose tolerance. The hypothesis of this study is that insulin secreted during cephalic phase would also have actions targeting microvascular tissue, with resulting capillary recruitment. Recent studies with healthy subjects have shown an association between microvascular function and dietary factors. Healthy eating patterns and nutritional interventions with specific food items represent non-pharmacological preventive and therapeutic strategies for reducing inflammation and cardio-metabolic associated risk factors. The study of food intake included in this research aimed to identify associations between dietary intake and microvascular function in healthy subjects. After clinical and laboratorial assessment, 39 healthy men underwent two nailfold videocapillaroscopies, with an interval of 10 minutes between them. In this interval, according to randomization, they were subjected to a great-looking breakfast tray (CPIS group) or to nothing (control group). Blood samples were drawn at 3, 9 and 15 minutes after the stimulus presentation to assess insulin and pancreatic polypeptide (PP) levels, which are markers of cephalic phae of digestion. Throughout the exam, microflow and vasomotion were measured by laser-Doppler flowmetry. After the exam insulin iontophoresis was performed. All participants answered a food-frequency questionnaire (FFQ) regarding their dietary intake in the last 12 months. The assessment of microvascular response to CPIS compared differences in functional capillary density (FCD) and microflow in the CPIS and control groups, pre- and post-stimulus and their correlations with changes in insulin and PP. The study of food intake attempted to identify correlations between dietary factors and functional capillary recruitment pre-stimulus in the total sample. After the interval, the CPIS group had significantly increased basal and peak FCD. Insulin variation was not different between groups. PP was significantly increased in the CPIS group and its variation was positively correlated with FCD increase. Resting red blood cell velocity (RBCV) and peak RBCV were also significantly increased and time taken to reach peak RBCV, decreased. The study of food intake revealed positive associations between calcium, selenium and dairy intakes and functional capillary recruitment in the total sample. In conclusion, skin microcirculation is activated during cephalic phase of digestion. Selenium, calcium and dairy intakes are positively associated with capillary recruitment in healthy men. A fase cefálica de secreção de insulina (FCSI) ocorre dentro da fase cefálica da digestão, em aproximadamente dez minutos entre a estimulação oro-sensorial pelo alimento e o início da absorção deste e determina um incremento rápido dos níveis basais de insulina. A FCSI parece ser importante para a tolerância normal à glicose. A hipótese deste estudo é de que a insulina secretada na fase cefálica da digestão teria ações direcionadas para o tecido microvascular, com conseqüente recrutamento capilar. Estudos recentes com indivíduos sadios têm mostrado a associação entre função microvascular e componentes dietéticos. Padrões alimentares saudáveis e intervenções nutricionais com alimentos específicos representam estratégias preventivas e terapêuticas não-farmacológicas para redução da inflamação e do risco metabólico e cardiovascular associados. O estudo de consumo alimentar inserido nesta pesquisa objetivou identificar associações entre componentes dietéticos e a função microvascular em indivíduos saudáveis. Após avaliação clínica e laboratorial, 39 voluntários saudáveis foram submetidos a dois exames de videocapilaroscopia do leito ungueal, com um intervalo de dez minutos entre os mesmos. Neste intervalo, conforme randomização, uma refeição com ótima apresentação e aroma foi apresentada (estímulo sensorial) ou não (controle). Coletas sangüíneas foram realizadas aos 3, 9 e 15 minutos após a apresentação do estimulo, para avaliação dos níveis de insulina e polipeptídeo pancreático (PP), marcadores bioquímicos da fase cefálica da digestão. Durante todo o exame medidas de fluxo e vasomotricidade foram realizadas pela técnica de laser-Doppler fluxometria. Após o exame, foi realizada iontoforese transdérmica de insulina. Todos os participantes responderam a um questionário de freqüência alimentar (QFA), relativo ao hábito de consumo dietético dos últimos 12 meses. O estudo da resposta microvascular à fase cefálica comparou as diferenças de densidade capilar funcional e fluxo capilar nos grupos experimental e controle, antes e após o estímulo sensorial, e sua correlação com a variação de insulina e PP. O estudo de consumo alimentar avaliou correlações entre componentes dietéticos e recrutamento capilar no exame basal (antes do estímulo) em toda amostra. Após o intervalo, o grupo que recebeu o estímulo sensorial teve sua densidade capilar funcional (DCF) aumentada. A variação de insulina não diferiu entre os grupos e a de PP foi maior no grupo estimulado, sendo também correlacionada positivamente como aumento na DCF. A velocidade máxima de perfusão pósisquemia também aumentou no grupo estimulado, enquanto o tempo para alcançar esta velocidade máxima diminuiu. O estudo do consumo alimentar revelou associações positivas entre o consumo de cálcio, selênio e laticínios e recrutamento capilar na amostra total. Em conclusão, o consumo alimentar de cálcio, selênio e laticínios foi associado com maior recrutamento capilar em indivíduos sadios. Nestes indivíduos a microcirculação cutânea respondeu ao estímulo da fase cefálica da digestão com recrutamento capilar e aumento de fluxo sanguíneo microvascular.
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- 2010
33. Relationship between body mass index and serum thyrotropin in euthyroid women: differences by smoking, race and menopause status
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Souza, Amanda de Moura, Sichieri, Rosely, Carvalho, Denise Pires de, Moraes, Claudia Leite, and Mendonça, Gulnar Azevedo e Silva
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Índice de massa corporal ,Tabagismo ,Race ,TSH ,Thyroid hormones ,CIENCIAS DA SAUDE::SAUDE COLETIVA [CNPQ] ,Raça ,Menopaude ,Body mass index ,Tobacco smoking ,Hormônios tireoidianos ,Menopausa ,Tirotrofina - Abstract
Submitted by Boris Flegr (boris@uerj.br) on 2020-07-05T16:10:21Z No. of bitstreams: 1 Souza, Amanda.pdf: 5446227 bytes, checksum: 8beaf8420938900ad32b4232ecfe3f1f (MD5) Made available in DSpace on 2020-07-05T16:10:21Z (GMT). No. of bitstreams: 1 Souza, Amanda.pdf: 5446227 bytes, checksum: 8beaf8420938900ad32b4232ecfe3f1f (MD5) Previous issue date: 2010-02-12 Conselho Nacional de Desenvolvimento Científico e Tecnológico Although, overt thyroid dysfunction is associated with weight changes, it is not known whether minor changes in thyroid function within normal serum thyrotropin (TSH) concentration could affect body mass index (BMI). The association between serum TSH and BMI in euthyroid subjects has been focus of recent and controversial studies. A possible explanation for these controversial findings could be the differences on this association between population subgroups. The aim of this study was to investigate the association between BMI and TSH in women with normal thyroid function and to evaluate a potential effect modification on the association between BMI and TSH by smoking, race, and menopause status. A population-based study was carried out in the city of Rio de Janeiro, Brazil, in 2004-2005. Sample selection was based on a three-stage cluster design. In the first stage, 100 primary sample units (PSU) were selected. In the second stage, 15 households were select from each PSU and in the third stage one woman was selected from each household. Of a sample of 1500 women aged 35 years or older, 1298 agree to participate. For the present study, a final sample of 1084 women without thyroid disease was investigated. Overall, BMI was positively and significantly associated with serum TSH (β=0.90; p- value=0.02) and stronger association between BMI and serum TSH among premenopausal women (β=1.04; p-value=0.04), Black women (β=1.39; p-value=0.14) and smokers (β=1.77; p-value=0.04) was found. In conclusion, TSH appears to modulate body weight among women even in the normal range. Smoking and race are strong effect modifiers of the association between TSH and BMI and future studies should take these effect modifiers into account. As disfunções tireoidianas estão associadas com alterações no peso corporal, no entanto, não está estabelecido na literatura se pequenas alterações na função tireoidiana dentro dos valores de referência das concentrações séricas de tireotrofina (TSH) poderiam afetar o índice de massa corporal (IMC). A associação entre as concentrações séricas de TSH e o IMC em indivíduos eutireóideos tem sido foco de recentes e conflitantes estudos e uma possível explicação para os achados conflitantes é a existência de subgrupos da população onde essa associação se expressa de forma diferente. O objetivo desse trabalho foi investigar a associação entre o IMC e as concentrações séricas de TSH em mulheres com função tireoidiana normal e avaliar possível modificação de efeito da associação entre IMC e as concentrações séricas de TSH pelo tabagismo, menopausa e raça. Os dados foram obtidos em um estudo de base populacional realizado na cidade do Rio de Janeiro, Brasil, entre junho de 2004 e abril de 2005, com amostragem probabilística por conglomerado em três estágios. No primeiro estágio foram selecionados 100 setores censitários, no segundo 15 domicílios de cada setor e no terceiro uma mulher por domicílio. Das 1500 mulheres com 35 anos ou mais selecionadas, 1298 participaram do estudo, dentre estas, 1084 apresentavam função tireoidiana normal. Uma associação positiva e estatisticamente significante foi observada entre o IMC e o TSH sérico (β=0,90; p-valor=0,02) na população como um todo. A análise exploratória de subgrupos com diferentes graus de associação foi obtida pela análise de interações estatísticas. Os subgrupos de mulheres na pré-menopausa (β=1,04; p-valor=0,04), de raça negra (β=1,39; p-valor=0,14) e fumantes (β=1,78; p-valor=0,04), apresentaram associações de maior magnitude. Em conclusão, o TSH parece ter um papel modulador das mudanças no peso corporal, mesmo em mulheres com função tireoidiana normal. O tabagismo e a raça são fortes modificadores de efeito da associação entre TSH e IMC e futuros estudos devem considerar estes fatores.
- Published
- 2010
34. Flavonoid rutin increases thyroid iodide uptake in rats.
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Gonçalves CF, Santos MC, Ginabreda MG, Fortunato RS, Carvalho DP, and Freitas Ferreira AC
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- Animals, Biological Transport drug effects, Male, Rats, Rats, Wistar, Symporters metabolism, Thyroid Gland physiology, Thyroid Hormones blood, Thyrotropin blood, Iodides metabolism, Rutin pharmacology, Thyroid Gland drug effects, Thyroid Gland metabolism
- Abstract
Thyroid iodide uptake through the sodium-iodide symporter (NIS) is not only an essential step for thyroid hormones biosynthesis, but also fundamental for the diagnosis and treatment of different thyroid diseases. However, part of patients with thyroid cancer is refractory to radioiodine therapy, due to reduced ability to uptake iodide, which greatly reduces the chances of survival. Therefore, compounds able to increase thyroid iodide uptake are of great interest. It has been shown that some flavonoids are able to increase iodide uptake and NIS expression in vitro, however, data in vivo are lacking. Flavonoids are polyhydroxyphenolic compounds, found in vegetables present in human diet, and have been shown not only to modulate NIS, but also thyroperoxidase (TPO), the key enzyme in thyroid hormones biosynthesis, besides having antiproliferative effect in thyroid cancer cell lines. Therefore, we aimed to evaluate the effect of some flavonoids on thyroid iodide uptake in Wistar rats in vivo. Among the flavonoids tested, rutin was the only one able to increase thyroid iodide uptake, so we decided to evaluate the effect of this flavonoid on some aspects of thyroid hormones synthesis and metabolism. Rutin led to a slight reduction of serum T4 and T3 without changes in serum thyrotropin (TSH), and significantly increased hypothalamic, pituitary and brown adipose tissue type 2 deiodinase and decreased liver type 1 deiodinase activities. Moreover, rutin treatment increased thyroid iodide uptake probably due to the increment of NIS expression, which might be secondary to increased response to TSH, since TSH receptor expression was increased. Thus, rutin might be useful as an adjuvant in radioiodine therapy, since this flavonoid increased thyroid iodide uptake without greatly affecting thyroid function.
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- 2013
- Full Text
- View/download PDF
35. [Clinical repercussion of the 6th edition TNM staging system classification on differentiated thyroid carcinoma].
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Varandas VM, Coelho SM, Soeiro AP, Coeli CM, Carvalho DP, Buescu A, and Vaisman M
- Subjects
- Adenocarcinoma, Follicular mortality, Adenocarcinoma, Follicular surgery, Adolescent, Adult, Aged, Carcinoma, Papillary mortality, Carcinoma, Papillary surgery, Epidemiologic Methods, Female, Humans, Lymph Nodes pathology, Lymphatic Metastasis, Male, Middle Aged, Neck Dissection, Neoplasm Invasiveness, Neoplasm Recurrence, Local, Neoplasm Staging, Thyroid Neoplasms mortality, Thyroid Neoplasms surgery, Thyroidectomy methods, Adenocarcinoma, Follicular pathology, Carcinoma, Papillary pathology, Thyroid Neoplasms pathology
- Abstract
The TNM classification of UICC is used for predicting the outcome of thyroid cancer. The 6th edition changed the description of primary tumor (T), regional lymph node (N) and the staging group. The aim of this study was to compare the ability of the 5th and the 6th editions to predict outcome. The two classifications were applied in a retrospective analysis of 90 patients from HUCFF. Sixty-nine patients had papillary carcinoma, 14 follicular, 4 Hürthle cell, and 3 mixed. Patients were followed for a mean period of 58.3 months. At the end of follow-up, 49 patients were disease-free, 23 persisted with disease, 4 had cervical recurrence, 11 had metastases and 3 died. According to the 6th edition, 19 patients were classified as T1, compared to 7 based on the 5th edition; 19 patients were T2 compared to 30; 14 were T3 compared to 10; 22 were T4 compared to 27, and 16 patients were Tx. Both editions showed comparable remissions for stages I, II, and III. For the stage IV there was a significant change in remission, however there was no difference comparing IV and IV C.
- Published
- 2007
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