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The article offers information on how to scale the UltraPerformance liquid chromatography (UPLC) impurity/degradant separation to a preparative LC separation. The UPLC separation of the ranitidine degradation sample succeeds because it was found that ranitidine is resolved from all other compounds in the mixture. Moreover, further optimization can be achieved by simple modification of the gradient profile.

The article presents information about SunFire™ C18 reversed-phase high performance liquid chromatography columns. These columns provide high efficiencies and symmetric peak shapes for the analysis of acids, neutrals and bases and they exhibit superior lifetimes under low pH conditions.

Crude synthetic peptide samples were separated on preparative Atlantis™ dc18 columns. Fraction collection was triggered by the mass-to-charge (rn/z) ratio of the peptides. which specified the target peptide in the collected fraction to increase the fraction purities. Mass-directed peptide purification was more robust than UV-based collection under overloading conditions, and increased the traction purities. LC-MS-MS analysis was further employed to identify the impurities in the crude synthetic peptide sample. This study demonstrates high mass loading, selective purification. high recoveries and purity, and the capability of utilizing mass-directed fractionation for purifying peptides on preparative RPLC columns. [ABSTRACT FROM AUTHOR]