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Polymeric nanoparticles are commonly used in several biological applications. There are limited number of studies on the toxicity and potential effectiveness of polymeric nanoparticles to need for further study on polymer science. The aim of the study was to investigate characterization, in vitro, in vivo toxicity and biological distribution of Lys-graft-p(HEMA) polymeric nanoparticle. The characterization analyses showed that Lys-graft-p(HEMA) had an average size of around 171 nm and zeta potential was −22.6 mV. The sample was recorded from 750 to 4000 cm−1 in FTIR spectroscopy and the characteristic peaks of stretching band were observed in the spectrum. The polymeric nanoparticle did not show any cytotoxic effect on human embryonic kidney 293 healthy cell line (HEK 293, ATCC-CRL-1573) after 24 and 48 h of exposure. The nanoparticle did not cause mutation effects on Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537 strains. In addition, it has not hemolitic activity on rabbit erythrocytes at applied concentration and no lethality was observed with single oral dose toxicity test. FITC-Lys-graft-p(HEMA) were observed on different organs such as liver, kidney, heart, lung and large intestine at 6. hours by in vivo imaging system. These results reveal that Lys-graft-p(HEMA) polymeric nanoparticles can be used as a potential drug carrier system because of its biocompatibility.

Isolation of DNA is one of the important processes for biotechnological applications such as investigation of DNA structures and functions, recombinant DNA preparations, identification of genetic factors and diagnosis and treatment of genetic disorders. The aim of this study was to synthesis and characterizes the galactoacrylate based nanopolymers with high surface area and to investigate the usability of these synthesized nanopolymers for DNA isolation studies. Nanopolymers were synthesized by the surfactant free emulsion polymerization technique by using the monomers of 2-hydroxyl ethylmethacrylate and 6-O-(2

In this study, the objective was to separate exopolysaccharides (EPSs) released in the broth subsequent to outdoor cultivation of Botryococcus braunii. For this, poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were synthesized. After that, the surface was modified by coupling Concanavalin A. Box-Behnken statistical design was used to evaluate the effect of freezing temperature, Con A concentration and flow rate on Con A binding capacity. Optimum synthesis conditions were elicited as -14.48°C freezing temperature, 1.00mg/ml Con A concentration and 0.30ml/min flow rate yielding 3.18mg Con A/g cryogel, whereas -16°C, 1.00mg/ml and 0.30ml/min yielded the highest (3.38mg) binding capacity in experimental cryogel preparation. The EPS adsorption capacity of the optimum cryogel column was found as 3.26mg EPS/g cryogel corresponding to adsorption yield of 80%. Besides; swelling test, elemental analysis, Micro-CT, SEM and FTIR analysis were carried out for characterization of the synthesized cryogels.

Aim: Formoterol, N-[2-hydroxy-5-(1-hydroxy-2-(4-methoxyphenyl)-1-methylethyl)amino ethyl] is a bronchodilator used in the treatment of obstructive airways diseases.With this preliminary study,we aimed to develop an effective interaction between formoterol and specifically designed nanoparticle based polymeric structures for it under laboratory conditions.This effort relates to developing a new generation medicinal aerosol formulations with nanocarriers in our following studies. Method: Considering abundance of structural rings of formoterol, we chose tryptophan as one of the well-known hydrophobic polymers. p(HEMA) (2-hydroxyethyl methacrylate) nanoparticles were prepared by surfactant free emulsion polymerization and then grafted with L-Tryptophan according to the procedure [1]. Results: L-Tryptophan graft p(HEMA)nanocarriers were characterized by SEM,FTIR,Zeta Sizer.System parameters such as medium pH,adsorption time,adsorption capacity,initial concentration of formoterol-fumarate were optimized. After the optimization of these parameters, formoterol-fumarate releases studying were carried out in different medium pH and drug release kinetic models were also determined. Conclusion: As a result of our initial laboratory experiments,we observed that there were intense hydrophobic interactions between Graft-Trp-p(HEMA) nanocarriers and formoterol.Preliminary findings of this ongoing study look promising. Formoterol and specifically designed Graft-Trp-p(HEMA) nanocarriers have got interaction on laboratory conditions, thus create a highly bioavailable formulation in the future could be possible.Yet the final optimization works still continue.