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Despite the cytopathic nature of influenza A virus (IAV) replication, we recently reported that a subset of lung epithelial club cells is able to intrinsically clear the virus and survive infection. However, the mechanisms that drive cell survival during a normally lytic infection remained unclear. Using a loss-of-function screening approach, we discovered that the DNA mismatch repair (MMR) pathway is essential for club cell survival of IAV infection. Repair of virally induced oxidative damage by the DNA MMR pathway not only allowed cell survival of infection, but also facilitated host gene transcription, including the expression of antiviral and stress response genes. Enhanced viral suppression of the DNA MMR pathway prevented club cell survival and increased the severity of viral disease in vivo. Altogether, these results identify previously unappreciated roles for DNA MMR as a central modulator of cellular fate and a contributor to the innate antiviral response, which together control influenza viral disease severity.

Building design, maintenance, and management can have significant impacts on accessibility and suitability for pest species. The brown marmorated stink bug, Halyomorpha halys (Stål), causes household nuisance pest problems because of its habit of using human homes as winter refuges. Studies were conducted to understand behaviors and characteristics relevant to this problem, including response to gravity, ability to pass through openings, responses to heat, and mortality estimations in wall assemblies. In the lab, winter shelter seeking bugs were shown to exhibit upward movement, and field observations of bugs on building exteriors gave similar results. In experiments testing the size limits on openings through which bugs could pass, height limited tests excluded most females at 4 mm, and all bugs at 3 mm. Pronotum width limited tests excluded most females at 8 mm and nearly all bugs at 7 mm. Accompanying measurements of over 900 bugs found an average female pronotum width of 8.33 mm and height of 4.03 mm, and male pronotum width of 7.47 mm and height of 3.50 mm, with minimum sizes indicating that only a small percentage of bugs will pass the smallest openings tested. Heat response experiments of shelter seeking bugs were first piloted outdoors, and then modified to be a forced choice indoor test. In outdoor tests on a flat plane wall section with alternating heated sections, bugs did not respond to thermal contrast but rather immediately walked off of the wall. In the indoor forced choice test, a box of four cavity walls was used. Bugs did not respond to the heated sections in either the adjacent or opposite configuration. Thermal simulation modeling was used to evaluate the possible effects of varying wall assembly materials and configurations on cold-related mortality of bugs overwintering in the cavity space behind cladding. Simulation results indicated that bugs electing to overwinter in the space between cladding and sheathing were at risk of freezing deaths, with mortality expectations increasing in better insulated buildings. The results of these studies will inform future control measures and impact studies in buildings. Ph. D.

The brown marmorated stink bug (Halyomorpha halys) (Stål) is known for overwintering in human homes. Studies were conducted to understand the behavior of adult bugs in response to characteristics of potential overwintering structures including gap sizes, light, and presence of dead conspecifics. In a test where bugs were placed in increasingly tight boxes, most bugs settled in gaps 4.5 - 5.5 mm high. None settled in a space less than 3.5 mm high. In boxes with constant tightness, bugs tended to move to the back, and primarily settled along walls and in corners. In both box types, bugs tended to turn and face the cavity entrance during settling. In tests of responses of disturbed overwintering bugs to common household light bulbs over the course of a year, bugs were taken from shelters and exposed to lit bulbs. Bug responses were seasonal, with attraction to light bulbs in spring and summer, but little or no consistent response in fall or winter. The lack of response occurred more often at night than in afternoons. Because not all bugs survive the winter, corpses can accumulate. Single shelter-seeking H. halys were found not to respond to the presence of dead aggregations from the previous years unless touch was possible, in which case aggregations were joined. Dead aggregations from the same year had a repellent effect. Presence of a single dead bug from the same year did not provide any survivorship advantage to overwintering bugs. Results suggest possible improvements to trap shelters. Master of Science in Life Sciences The brown marmorated stink bug (Halyomorpha halys) is known for overwintering in human homes. This research investigated the responses of these bugs to some characteristics of overwintering structures, including crevice tightness preference, light sources, and dead bugs leftover from previous years. Tightness experiments indicated that bugs in crevices with hard walls prefer to settle in spaces between 4.5 and 5.5 mm high. Bugs also tended to stay on the floor, go back as far as they could, and face the entrance of the crevice. These findings will help improve overwintering box designs for collection and research, and will help pest control professionals focus their efforts. When bugs were disturbed and exposed to common household light bulbs, they tended to move toward the bulbs in late summer and in spring, but responded less in fall and winter, and responded more often in the afternoon than at night. Bugs were also exposed to other dead bugs in several ways. When lone bugs were exposed to groups of dead bugs from previous years, they went to them only if they could touch them. Single dead bugs had less of an impact. Exposure to groups of dead bugs from the same year drove live bugs away. Overwintering brown marmorated stink bugs do not appear to eat other bugs that have recently died. These results suggest several possible improvements to the designs of trap shelters used for research and control.

This manuscript provides an understanding of water conservation related innovations in green buildings, both in terms of what is being selected in design phase and how professionals perceive their experiences with these innovations. The innovations examined include toilets, showers, sinks, plumbing, water heating, appliances, alternative water sources, landscaping, performance monitoring, and user education. It contains a literature review of unanticipated consequences associated with these innovations, and creates a framework for categorizing these based on a synthesis of the literature of unanticipated consequences. A review of certification documents from the Leadership in Energy and Environmental Design (LEED) rating system identifies what landscaping, toilet, and shower innovations are most commonly designed for in LEED certified buildings. These data are also used to identify differences in innovation selection across climate regimes. An internet survey of green building professionals provides a picture of satisfaction with these innovations in practice. It also gives examples of these experiences so that future users can take advantage or take caution as necessary. Master of Science