174 results on '"Chang-Hong Li"'
Search Results
2. Acute, chronic, and genotoxic studies on the protopine total alkaloids of the Macleaya cordata (willd.) R. Br. in rodents
- Author
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Zhen Dong, Shu-sheng Tang, Xiao-lan Ma, Bin Tan, Zhao-shan Tang, Chang-hong Li, Zi-hui Yang, and Jian-guo Zeng
- Subjects
Macleaya cordata (willd.) R. Br. ,protopine alkaloids ,acute toxicity ,chronic toxicity ,mutagenicity ,teratogenicity ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The protopine alkaloids are widely distributed within the opium poppy family and have a wide range of pharmacological effects. MPTA is a product of the protopine total alkaloids extracted from the Macleaya cordata (Willd.) R. Br. Previously, we reported good anti-inflammatory activity of MPTA as well as oral acute and sub-chronic toxicity studies in rats. In order to perform a systematic toxicological safety assessment of MPTA, oral acute toxicity, genotoxicity (bone marrow cell chromosome aberration test, sperm abnormality test, bone marrow cell micronucleus test, and rat teratogenicity test), and chronic toxicity in mice were performed in this study. In the oral acute toxicity test, the LD50 in ICR mice was 481.99 mg/kg, with 95% confidence limits ranging from 404.27 to 574.70 mg/kg. All three mutagenicity tests tested negative in the range of 60.25–241.00 mg/kg. The results of the teratogenicity test in rats showed no reproductive or embryonic developmental toxicity at only 7.53 mg/kg, which can be considered as a no observed effect level (NOEL) for the teratogenicity test. Therefore, MPTA is safe for use at the doses tested, but attention should be paid to the potential risk to pregnant animals and the safety evaluation and toxicity mechanisms in target animals should be further investigated.
- Published
- 2022
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- View/download PDF
3. Preclinical safety evaluation of Macleaya Cordata extract: A re-assessment of general toxicity and genotoxicity properties in rodents
- Author
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Zhen Dong, Shu-Sheng Tang, Xiao-Lan Ma, Chang-Hong Li, Zhao-Shan Tang, Zi-Hui Yang, and Jian-Guo Zeng
- Subjects
Macleaya cordata ,extract ,rodent ,acute toxicity ,long-term toxicity ,genotoxicity ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Macleaya cordata extract (MCE) is widely used for its diverse pharmacological actions and beneficial effects on farm animals. Modern pharmacological studies have shown that it has anti-inflammatory, anti-cancer, and anti-bacterial activities, and is gradually becoming a long-term additive veterinary drug used to improve animal intestinal health and growth performance. Although some evidence points to the DNA mutagenic potential of sanguinarine (SAN), a major component of MCE, there is a lack of sufficient basic toxicological information on the oral route, posing a potential safety risk for human consumption of food of animal origin. In this study, we assessed the acute oral toxicity, repeated 90-day oral toxicity and 180-day chronic toxicity of MCE in rats and mice and re-evaluated the genotoxicity of MCE using a standard combined in vivo and ex vivo assay. In the oral acute toxicity test, the LD50 for MCE in rats and mice was 1,564.55 mg/kg (95% confidence interval 1,386.97–1,764.95 mg/kg) and 1,024.33 mg/kg (95% confidence interval 964.27–1,087.30 mg/kg), respectively. The dose range tested had no significant effect on hematology, clinical chemistry, and histopathological findings in rodents in the long-term toxicity assessment. The results of the bacterial reverse mutation, sperm abnormality and micronucleus test showed negative results and lack of mutagenicity and teratogenicity; the results of the rat teratogenicity test showed no significant reproductive or embryotoxicity. The results indicate that MCE was safe in the dose range tested in this preclinical safety assessment. This study provides data to support the further development of maximum residue limits (MRLs) for MCE.
- Published
- 2022
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4. Exploring the Anti-inflammatory Effects of Protopine Total Alkaloids of Macleaya Cordata (Willd.) R. Br.
- Author
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Zhen Dong, Yu-hong Wang, Zhao-shan Tang, Chang-hong Li, Tao Jiang, Zi-hui Yang, and Jian-guo Zeng
- Subjects
Macleaya cordata (Willd.) R. Br. ,protopine total alkaloids ,acute inflammation ,anti-inflammatory ,network pharmacology ,molecular docking ,Veterinary medicine ,SF600-1100 - Abstract
Macleaya cordata (Willd). R. Br. is a Chinese medicinal plant commonly used externally to treat inflammatory-related diseases such as arthritis, sores, and carbuncles. This study aimed to evaluate the anti-inflammatory activity of protopine total alkaloids (MPTAs) in Macleaya cordata (Willd.) R. Br. in vivo tests in rats with acute inflammation showed that MPTA (2.54 and 5.08 mg/kg) showed significant anti-inflammatory activity 6 h after carrageenan injection. Similarly, MPTA (3.67 and 7.33 mg/kg) showed significant anti-inflammatory activity in the mouse ear swelling test. In addition, the potential mechanisms of the anti-inflammatory effects of MPTA were explored based on network pharmacology and molecular docking. The two main active components of MPTA, protopine and allocryptopine, were identified, and the potential targets and signaling pathways of MPTA's anti-inflammatory effects were initially revealed using tools and databases (such as SwissTargetPrediction, GeneCards, and STRING) combined with molecular docking results. This study provides the basis for the application of MPTA as an anti-inflammatory agent.
- Published
- 2022
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5. Ablation of GSDMD Attenuates Neurological Deficits and Neuropathological Alterations After Traumatic Brain Injury
- Author
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Hao Du, Chang-Hong Li, Ruo-Bing Gao, Xiao-Qing Cen, and Ping Li
- Subjects
GSDMD ,NLRP3 inflammasome ,traumatic brain injury ,neuroinflammation ,neuropathology ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Pyroptosis plays a significant role in neuroinflammation after traumatic brain injury (TBI). However, the role of pyroptosis executor Gasdermin D (GSDMD) in neurological deficits and neuropathological alterations after TBI have not been elucidated. Our results demonstrated that GSDMD-KO exerted striking neuroprotective effects on motor dysfunction and neuropathological alterations (loss of synaptic proteins, microglia activation, astrogliosis, dendrite injury, and neuron death) at 3 days after TBI. GSDMD-KO inhibited the expression and release of pro-inflammatory cytokine releases (IL-1β and TNF-α) while promoting those of anti-inflammatory cytokines (IL-10 and TGF-β1). The temporal pattern of diverse inflammasome signals showed long-lasting elevations of NLRP3, caspase 1, and caspase 1 p20 after TBI, rather than NLRP1, NLRC4 or AIM2, similar to the change in GSDMD postinjury; and NLRP3-KO not only inhibited the expression and cleavage of GSDMD but also attenuated the loss of synaptic proteins and neurological deficits. Notably, RNA sequencing showed both GSDMD-KO and NLRP3-KO reversed the global expression of neuroinflammation- and neuropathology-related genes after TBI. Our findings proved that the inhibition of GSDMD exerts neuroprotective effects after TBI and is mainly driven by the NLRP3 inflammasome. GSDMD serves as a potent therapeutic target for the treatment of TBI.
- Published
- 2022
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6. Effect Of Open-Pit Blasting Vibrations on a Hanging-Wall Slope: A Case Study of the Beizhan Iron Mine in China
- Author
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Yong-gang Xiao, Jie Cao, Xiao-min Liu, and Chang-hong Li
- Subjects
Geology ,QE1-996.5 - Abstract
With the increase in the depth of large-scale open-pit mining, many mines have to face problems such as what is the effect of open-pit blasting on the rock slope and how to ensure the stability of a high-steep slope. The east slope of the Beizhan iron mine in Hejing County, Xinjiang, belongs to the typical open-pit high and steep slope of mined hanging-wall ore. To study the effect of open-pit blasting vibrations on hanging-wall slope stability, the intelligent blasting vibration detector was used to monitor the open-pit blasting wave of the Beizhan iron mine and the corresponding numerical model was established. We fitted the transmission law of slope blasting vibration by Sodev’s regression formula and calculated stress, strain, and vibration velocity of the whole slope by numerical simulation. The result showed that the fitting functional relationship was correct and could be the basic rule of predicting the maximum charge amount per delay interval and minimum safe distance for this area. The estimated open-pit blasting charge weight was reasonable and blasting vibrations would have little effect on the hanging-wall slope. The research method and conclusions in this article have numerous reference values for studying the hanging-wall slope’s kinetic stability.
- Published
- 2022
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7. Nitazoxanide, an Antiprotozoal Drug, Reduces Bone Loss in Ovariectomized Mice by Inhibition of RANKL-Induced Osteoclastogenesis
- Author
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Chang-hong Li, Zi-rui Lü, Zhen-da Zhao, Xin-yu Wang, Hui-jie Leng, Yan Niu, and Mo-pei Wang
- Subjects
nitazoxanide ,stat3 ,osteoclastogenesis ,Nfatc1 ,osteoporosis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Nitazoxanide (NTZ) is an FDA-approved anti-parasitic drug with broad-spectrum anti-infective, anti-inflammatory, and antineoplastic potential. However, its regulatory effects on osteoclastogenesis and the underlying mechanisms remain unclear. The present study found that NTZ potently inhibited osteoclast formation at the early stage of receptor activator of NF-κB ligand-induced osteoclastogenesis in a concentration-dependent manner at a non-growth inhibitory concentration. NTZ suppressed actin ring formation and decreased osteoclast marker gene expression, including TRAP, MMP9, and cathepsin K. NTZ significantly impaired the bone resorption activity of osteoclasts. In vivo, ovariectomized mice were treated with 50, 100 and 200 mg/kg/d NTZ for 3 months. NTZ (100 mg/kg/d) administration markedly reduced ovariectomy-induced bone loss by suppressing osteoclast activity. Mechanistically, osteoclastogenesis blockade elicited by NTZ resulted from inhibition of STAT3 phosphorylation, and reduction of the Ca2+ fluorescence intensity and NFATc1 expression. NTZ weakened the binding between STAT3 and the NFATc1 promoter region. Furthermore, enforced NFATc1 expression partly rescued the impaired osteoclast differentiation in NTZ-treated RAW264.7 cells. In summary, NTZ could inhibit osteoclastogenesis and bone loss through modulation of the receptor activator of NF-κB ligand-induced STAT3-NFATc1 signaling pathway, which might be a potential alternative treatment regimen against bone destruction-related diseases including osteoporosis.
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- 2021
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8. Investigation of the Effects of Freeze-Thaw Cycles on Geomechanical and Acoustic Characteristics of Tuff Specimens under Different Stress Paths
- Author
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Yong-gang Xiao, Chang-hong Li, Jie Cao, Yu Wang, Zhi-qiang Hou, and Nan Hu
- Subjects
Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
In the process of development and construction of open-pit mine slope in the high altitude and cold area, freeze-thaw (F-T) cycles have an important impact on rock engineering structure. F-T cycles lead to the decrease in physical and mechanical properties of rock, which is closely related to the stability of open-pit slope. In this paper, the influence of F-T cycles on geomechanical and acoustic emission (AE) characteristics of tuff specimens under different stress paths was studied by using F-T cycle treatment, in situ AE monitoring, and uniaxial loading test. The results indicated that under the same stress path, the cumulative AE count/energy of rock samples subjected to F-T cycles was less than that of rock samples not subjected to F-T cycles. The peak frequency distribution of AE signal during the loading process of rock specimen presented the phenomenon of frequency bands. The width of the low-frequency band of the rock samples subjected to F-T cycles was larger than that of the rock samples under the natural state. The frequency and width of the high-frequency band of the rock samples subjected to F-T cycles were larger than those of the rock samples under the natural state. The rock samples subjected to F-T cycles had higher plastic strain than those without F-T cycles. According to the uniaxial compression test results of F-T rock samples under different stress paths, the peak stress and peak strain have little change, but the AE characteristics were obviously different.
- Published
- 2020
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9. Characterization of the complete chloroplast genome of Liquidambar acalycina Chang
- Author
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Jiu-Xin Lai, Fu-Rong Lin, Ping Huang, Chang-Hong Li, and Yong-Qi Zheng
- Subjects
liquidambar acalycina ,chloroplast genome ,phylogenetic analysis ,Genetics ,QH426-470 - Abstract
Liquidambar acalycina is a popular tree species with high ornamental and economic values because of the leaf shape and coloration. In this study, chloroplast genome of L.acalycina was assembled using Illumina highlights pair-end sequencing data. The chloroplast genome has a quadripartite structure with 160,433 bp in length. It contains a pair of 26,274 bp inverted repeat (IR) regions, which were separated by large single-copy (LSC: 88,963bp) region and small single-copy (SSC: 18,922 bp) region. A total of 133 genes were annotated, including 88 protein-coding genes (PCGs), 37 tRNAs, and 8 rRNAs. The overall GC content was 36.1%. The phylogenetic analysis revealed that L. acalycina has a close relationship with L. fomosana. This complete chloroplast genome is of great value in the genetic research of L. acalycina.
- Published
- 2020
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10. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells
- Author
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Xin-Jiang Lu, Qiang Chen, Ye-Jing Rong, Guan-Jun Yang, Chang-Hong Li, Ning-Yi Xu, Chao-Hui Yu, Hui-Ying Wang, Shun Zhang, Yu-Hong Shi, and Jiong Chen
- Subjects
Science - Abstract
How extramedullar cytokines regulate hematopoietic stem cell (HSC) homeostasis is unclear. Here, the authors show that the cytokine leukocyte cell-derived chemotaxin 2 regulates HSC expansion and mobilisation via tumour necrosis factor and interaction with CD209a in macrophages.
- Published
- 2016
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11. Analysis of the Sedimentation Characteristics of Ultrafine Tailings Based on an Orthogonal Experiment
- Author
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Chang-hong Li, Yue-qi Shi, Peng Liu, and Ning Guo
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Due to continuous improvements in the beneficiation process, the size of tailings has decreased. In many mines, more than 50% of the total tailings are finer than 74 μm. Ultrafine tailings exhibit a slow settling velocity and uneven settling, which pose new challenges to the safety and stability of tailings dams. In this paper, ultrafine iron tailings from the Makeng Iron Mine in Longyan City, Fujian Province, were used as the research object. A four-factor, three-level orthogonal test method was used to study the sedimentation characteristics of ultrafine tailings with four common curing agent materials, including polyacrylamide, polyaluminum chloride, polyaluminum sulfate, and polyferric sulfate. The results show that when the pulp concentration is 30%, the polyacrylamide is cationic and the molecular weight is 10 million, the optimal content of the flocculant is 3‰, the optimum dose of the polyaluminum chloride with a content of 28 is 0.1‰, the optimum dose of polyaluminum sulfate is 1‰, and the optimum dose of polymeric ferric sulfate is 1‰. When the flocculant is mixed according to the proportion of 2‰ polyacrylamide, 0.05‰ polyaluminum chloride, 1‰ polyaluminum sulfate, and 1.0‰ polyferric sulfate, the sedimentation speed of the ultrafine tailings is fast, and the supernatant liquid is clear. The results of multivariate nonlinear regression analysis of the sedimentation curve show that the primary and secondary factors affecting sedimentation are polyacrylamide > polyaluminum sulfate > polyaluminum chloride > polyferric sulfate. When the optimal ratio is applied, the cohesion (c) of ultrafine tailings increases from 27 kPa to 68.75 kPa and the internal friction angle (φ) increases from 25.53° to 27.53°, which shows that the shear strength improves and the stability of the tailings dam increases. The economic analysis of the composite flocculant with the optimal ratio shows that the flocculant with the optimal proportion has an obvious economic advantage over polyacrylamide alone.
- Published
- 2019
- Full Text
- View/download PDF
12. Characterization of the complete chloroplast genome of Dalbergia cultrata (Leguminosae)
- Author
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Yu Liu, Ping Huang, Chang-Hong Li, Feng-Qi Zang, and Yong-Qi Zheng
- Subjects
dalbergia cultrata ,chloroplast genome ,phylogenetic analysis ,Genetics ,QH426-470 - Abstract
Dalbergia cultrata is a Near Threatened species with high ecological and economic values. In this study, its chloroplast genome was assembled using Illumina pair-end sequencing dataset. The chloroplast genome has a quadripartite structure with 156,385 bp in length and contains a pair of 16,392 bp inverted repeat (IR) regions, which were separated by large single copy (LSC: 86,040 bp) region and small single copy (SSC: 37,561 bp) region. A total of 121 genes were annotated, including 77 protein-coding genes (PCGs), 36 tRNAs, and 8 rRNAs. The overall GC content was 36.1%. The phylogenetic analysis revealed that D. cultrata has close relationship to D. hainanensis and D. odorifera. This complete chloroplast genome can be readily used for population genetic studies of D. cultrata.
- Published
- 2019
- Full Text
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13. The MBD4 Gene Plays an Important Role in Porcine Adipocyte Differentiation
- Author
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Lian-Jiang Zhang, Ya-Nan Zhu, Yan Gao, Si-Yuan Liu, Bo Zhai, Chang-Hong Li, Hui-Yu Liu, Jian Chen, Bao Yuan, Li-Sheng Dai, and Jia-Bao Zhang
- Subjects
Pig ,MBD4 ,Adipocyte differentiation ,RNAi ,Methylation ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background: MBD4 (methyl-CpG binding domain protein 4) is an important G: T glycosylase that can identify T-G mismatches. It plays a role in active demethylation through base excision repair. Overexpression of MBD4 gene can cause the demethylation of numerous genes, and the remethylation of MBD4-associated genes can occur when the MBD4 gene is knocked out. To date, the functions and regulatory mechanisms of the MBD4 gene in the differentiation of porcine preadipocytes have not been clearly established. Methods: Subcutaneous fat cells from 1- to 7-day-old Junmu-1 piglets were cultured in vitro, induced to differentiate, and then identified. A real-time fluorescence-based quantitative polymerase chain reaction (PCR) analysis was conducted to detect MBD4 messenger RNA (mRNA) expression. Cells were treated with MBD4-siRNA (small interfering RNA) and induced to differentiate. Changes in the lipid droplets were observed by oil red O staining. Changes in the mRNA and protein expression levels of MBD4 and the adipose differentiation-associated genes C/EBPα (CCAAT-enhancer-binding protein alpha), PPARγ (peroxisome proliferator-activated receptor gamma), and aP2 (adipocyte protein 2) were detected. In addition, the bisulfite sequencing method was used to detect changes in methylation in the promoters of certain genes associated with adipose differentiation. Results: Levels of MBD4 mRNA and protein expression varied with time over the course of the porcine adipocyte differentiation, with the highest levels of this expression observed on day two of the differentiation process. After silencing MBD4 and inducing differentiation, the production of lipid droplets decreased, the mRNA expression levels of C/EBPα, PPARγ, and aP2 were significantly reduced, and DNA methylation modification levels were significantly elevated in the examined promoter regions. Conclusion: The silencing of the MBD4 gene can influence the DNA methylation levels of preadipocyte differentiation-related genes and subsequently inhibit the differentiation of porcine preadipocytes.
- Published
- 2014
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14. Molecular characterization of mudskipper (Boleophthalmus pectinirostris) hypoxia-inducible factor-1α (HIF-1α) and analysis of its function in monocytes/macrophages.
- Author
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Feng Guan, Xin-Jiang Lu, Chang-Hong Li, and Jiong Chen
- Subjects
Medicine ,Science - Abstract
Hypoxia-inducible factor-1α (HIF-1α) plays a critical role in immune and inflammatory responses and is important in controlling a variety of processes in monocytes and macrophages. However, very little information is available about the functions of HIF-1α in fish monocytes/macrophages (MO/MФ). In this study, the cDNA sequence of the mudskipper (Boleophthalmus pectinirostris) HIF-1α gene (BpHIF-1α) was determined. Sequence comparison and phylogenetic tree analysis showed that BpHIF-1α is clustered in the fish HIF-1α tree. Constitutive expression of BpHIF-1α mRNA was detected by real-time quantitative PCR in all tested tissues, and the expression was found to be dramatically increased in the skin, liver, spleen, and kidney after Edwardsiella tarda infection. In addition, hypoxia and infection induced the expression of the BpHIF-1α transcript and protein in MO/MФ, respectively. Hypoxia caused an increase in phagocytic and bactericidal capacity of mudskipper MO/MФ in a BpHIF-1α-dependent manner. BpHIF-1α induced an anti-inflammatory status in MO/MФ upon E. tarda infection and hypoxia. Therefore, BpHIF-1α may play a predominant role in the modulation of mudskipper MO/MФ function in the innate immune system.
- Published
- 2017
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15. Knockdown of adenosine A2A receptors in hippocampal neurons prevents post-TBI fear memory retrieval
- Author
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Xiao-Qing Cen, Ping Li, Bo Wang, Xing Chen, Yan Zhao, Nan Yang, Yan Peng, Chang-Hong Li, Ya-Lei Ning, and Yuan-Guo Zhou
- Subjects
Developmental Neuroscience ,Neurology - Published
- 2023
16. Molecular and functional characterization of a ladderlectin-like molecule from ayu (Plecoglossus altivelis)
- Author
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Wei Wang, Mei-Yi Liu, Chen-Jie Fei, Chang-Hong Li, and Jiong Chen
- Subjects
Environmental Chemistry ,General Medicine ,Aquatic Science - Abstract
Ladderlectin is a member of C-type lectins (CTLs) in teleost fish and involved in innate immune defense. In this study, ayu (Plecoglossus altivelis) ladderlecin-like (PaLL-like) sequence was cloned, which encodes a polypeptide of 172 amino acids that includes a signal peptide and characteristic C-type lectin-like domains (CTLDs). Phylogenetically, PaLL-like was most closely related to its teleost counterpart from shishamo smelt (Spirinchus lanceolatus). Expression analysis revealed a ubiquitous expression profile, with highest expression detected in liver and its expression was up-regulated following Vibiro anguillarum infection. Similar to canonical CTLs, PaLL-like exhibited carbohydrate-binidng capacities to a wide range of well-defined mono-/di-saccharides and likely confer PaLL-like the ability to agglutinate all tested bacterial, including three Gram-positive species (i.e., Listeria monocytogenes, Staphylococcus aureus and Streptococcus iniae) and eight Gram-negative species (i.e., Edwardsiella tarda, Aeromonas (A.) hydrophila, Escherichia coli, Vibrio (V.) harveyi, V. anguillarum, V. parahemolyticus, A. versoni and V. vulnificus), in a calcium-dependent manner. Further functional studies revealed that PaLL-like displayed immunomodulatory activities leading to enhanced bactericidal activity of serum, pathogen opsonization and macrophage activation with increased expression of pro-inflammatory cytokines (i.e., PaIL-1β and PaTNF-α). Collectively, these immunomodulatory activities of PaLL-like suppressed proliferations of V. anguillarum in targeted tissued in vivo and likely contributed to the increased survival rate of infected-fish. Overall, our results demonstrated PaLL-like is a critical component of innate immunity and provides protective effects against bacterial infection.
- Published
- 2022
17. Molecular characterization and antimicrobial activity of NK-lysin in black scraper (Thamnaconus modestus)
- Author
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Mei-Yi Liu, Yi-Rong Zhang, Jian-Hua Zhang, Liang Miao, Yun-Fei Dang, Chen-Jie Fei, Chang-Hong Li, and Jiong Chen
- Subjects
Environmental Chemistry ,General Medicine ,Aquatic Science - Published
- 2023
18. Numerical simulation of fracture failure characteristics of rock mass with multiple parallel fractures under seepage stress coupling
- Author
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Peng-fei Wang, Chang-hong Li, and Wen-hui Tan
- Subjects
General Earth and Planetary Sciences ,General Environmental Science - Published
- 2022
19. Similar simulation test study on permeability evolution mechanism of fault sliding fracture zone
- Author
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Peng-fei Wang, Jing Xu, and Chang-hong Li
- Subjects
General Earth and Planetary Sciences ,General Environmental Science - Published
- 2022
20. MOSPD2 is a receptor mediating the LEAP-2 effect on monocytes/macrophages in a teleost, Boleophthalmus pectinirostris
- Author
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Chang-Hong Li, Jie Chen, Li Nie, and Jiong Chen
- Subjects
rna interference ,yeast two-hybrid ,lcsh:Zoology ,leap-2 ,lcsh:QL1-991 ,monocyte/macrophage ,mospd2 - Abstract
Liver-expressed antimicrobial peptide 2 (LEAP-2) is a cationic peptide that plays an important role in a host’s innate immune system. We previously demonstrated that mudskipper (Boleophthalmus pectinirostris) LEAP-2 (BpLEAP-2) induces chemotaxis and activation of monocytes/ macrophages (MO/MФ). However, the molecular mechanism by which BpLEAP-2 regulates MO/MΦ remains unclear. In this study, we used yeast two-hybrid cDNA library screening to identify mudskipper protein(s) that interacted with BpLEAP-2, and characterized a sequence encoding motile sperm domain-containing protein 2 (BpMOSPD2). The interaction between BpLEAP-2 and BpMOSPD2 was subsequently confirmed by co-immunoprecipitation (Co-IP). Sequence analyses revealed that the predicted BpMOSPD2 contained an N-terminal extracellular portion composed of a CRAL-TRIO domain and a motile sperm domain, a C-terminal transmembrane domain, and a short cytoplasmic tail. Phylogenetic tree analysis indicated that BpMOSPD2 grouped tightly with fish MOSPD2 homologs and was most closely related to that of the Nile tilapia (Oreochromis niloticus). The recombinant BpMOSPD2 was produced by prokaryotic expression and the corresponding antibody was prepared for protein concentration determination. RNA interference was used to knockdown BpMOSPD2 expression in the mudskipper MO/MФ, and the knockdown efficiency was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. Knockdown of BpMOSPD2 significantly inhibited BpLEAP-2-induced chemotaxis of mudskipper MO/MФ and BpLEAP-2-induced bacterial killing activity. Furthermore, knockdown of BpMOSPD2 inhibited the effect of BpLEAP-2 on mRNA expression levels of BpIL-10, BpTNFα, BpIL-1β, and BpTGFβ in MO/MФ. In general, BpMOSPD2 directly interacted with BpLEAP-2, and mediated the effects of BpLEAP-2 on chemotaxis and activation of mudskipper MO/MФ. This is the first identification of MOSPD2 as a receptor for LEAP-2.
- Published
- 2020
21. Inflorescence and floral development in Ranunculus and three allied genera in Ranunculeae (Ranunculoideae, Ranunculaceae)
- Author
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Zhao, Liang, Bachelier, Julien B., Chang, Hong-li, Tian, Xian-hua, and Ren, Yi
- Published
- 2012
22. Floral development of Dichocarpum, Thalictrum, and Aquilegia (Thalictroideae, Ranunculaceae)
- Author
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Ren, Yi, Gu, Tian-qi, and Chang, Hong-li
- Published
- 2011
23. Analysis of the Sedimentation Characteristics of Ultrafine Tailings Based on an Orthogonal Experiment
- Author
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Peng Liu, Chang-hong Li, Ning Guo, and Yue-qi Shi
- Subjects
Flocculation ,Materials science ,Tailings dam ,Article Subject ,Polyacrylamide ,0211 other engineering and technologies ,General Engineering ,02 engineering and technology ,010501 environmental sciences ,Sedimentation ,Pulp and paper industry ,01 natural sciences ,Tailings ,chemistry.chemical_compound ,Settling ,chemistry ,lcsh:TA401-492 ,medicine ,Ferric ,lcsh:Materials of engineering and construction. Mechanics of materials ,General Materials Science ,Sulfate ,021102 mining & metallurgy ,0105 earth and related environmental sciences ,medicine.drug - Abstract
Due to continuous improvements in the beneficiation process, the size of tailings has decreased. In many mines, more than 50% of the total tailings are finer than 74 μm. Ultrafine tailings exhibit a slow settling velocity and uneven settling, which pose new challenges to the safety and stability of tailings dams. In this paper, ultrafine iron tailings from the Makeng Iron Mine in Longyan City, Fujian Province, were used as the research object. A four-factor, three-level orthogonal test method was used to study the sedimentation characteristics of ultrafine tailings with four common curing agent materials, including polyacrylamide, polyaluminum chloride, polyaluminum sulfate, and polyferric sulfate. The results show that when the pulp concentration is 30%, the polyacrylamide is cationic and the molecular weight is 10 million, the optimal content of the flocculant is 3‰, the optimum dose of the polyaluminum chloride with a content of 28 is 0.1‰, the optimum dose of polyaluminum sulfate is 1‰, and the optimum dose of polymeric ferric sulfate is 1‰. When the flocculant is mixed according to the proportion of 2‰ polyacrylamide, 0.05‰ polyaluminum chloride, 1‰ polyaluminum sulfate, and 1.0‰ polyferric sulfate, the sedimentation speed of the ultrafine tailings is fast, and the supernatant liquid is clear. The results of multivariate nonlinear regression analysis of the sedimentation curve show that the primary and secondary factors affecting sedimentation are polyacrylamide > polyaluminum sulfate > polyaluminum chloride > polyferric sulfate. When the optimal ratio is applied, the cohesion (c) of ultrafine tailings increases from 27 kPa to 68.75 kPa and the internal friction angle (φ) increases from 25.53° to 27.53°, which shows that the shear strength improves and the stability of the tailings dam increases. The economic analysis of the composite flocculant with the optimal ratio shows that the flocculant with the optimal proportion has an obvious economic advantage over polyacrylamide alone.
- Published
- 2019
24. Molecular characterization of the NK-lysin in a teleost fish, Boleophthalmus pectinirostris: Antimicrobial activity and immunomodulatory activity on monocytes/macrophages
- Author
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Jiong Chen, Fei-Fei Ding, and Chang-Hong Li
- Subjects
Fish Proteins ,0301 basic medicine ,Takifugu rubripes ,Proteolipids ,Lysin ,Spleen ,Aquatic Science ,Monocytes ,Microbiology ,Fish Diseases ,03 medical and health sciences ,Immune system ,Anti-Infective Agents ,medicine ,Animals ,Immunologic Factors ,Environmental Chemistry ,Amino Acid Sequence ,Edwardsiella tarda ,Phylogeny ,Vibrio ,Vibrio alginolyticus ,biology ,Vibrio harveyi ,Gene Expression Profiling ,Macrophages ,Vibrio parahaemolyticus ,Enterobacteriaceae Infections ,Fishes ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Immunity, Innate ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Vibrio Infections ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Sequence Alignment - Abstract
NK-lysin (NKL) is a cationic host defense peptide that plays an important role in host immune responses against various pathogens. However, the immunomodulatory activity of NKL in fishes is rarely investigated. In this study, we characterized a cDNA sequence encoding an NK-lysin homolog (BpNKL) from the fish, mudskipper (Boleophthalmus pectinirostris). Sequence analysis revealed that BpNKL is most closely related to tiger puffer (Takifugu rubripes) NKL. BpNKL transcript was detected in all the tested tissues, with the highest level in the gill, followed by the spleen and kidney. Upon Edwardsiella tarda infection, the mRNA expression of BpNKL in the mudskipper was significantly upregulated in the spleen, kidney, and gill. A shortened peptide derived from BpNKL, BpNKLP40, was then chemically synthesized and its biological functions were investigated. BpNKLP40 exhibited a direct antibacterial activity against some Gram-negative bacteria, including E. tarda, Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio harveyi, and induced hydrolysis of E. tarda genomic DNA. Intraperitoneal injection of 1.0 μg/g BpNKLP40 significantly improved the survival of mudskipper following E. tarda infection and reduced the bacterial burden in tissues and blood. Moreover, 1.0 μg/ml BpNKLP40 treatment had an enhanced effect on the intracellular killing of E. tarda by monocytes/macrophages (MO/MФ) as well as on the mRNA expression of pro-inflammatory cytokines in MO/MФ. In conclusion, our study reveals that BpNKL plays a role against E. tarda infection in the mudskipper by not only directly killing bacteria but also through an immunomodulatory activity on MO/MФ.
- Published
- 2019
25. Molecular characterization and chemotaxis assay of a CC motif chemokine ligand 25 from Japanese sea bass (Lateolabrax japonicus)
- Author
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Ling-Ling Zhang, Jiong Chen, and Chang-Hong Li
- Subjects
Fish Proteins ,Chemokine ,biology ,Vibrio harveyi ,Chemotaxis ,Lateolabrax ,General Medicine ,Aquatic Science ,Japanese sea bass ,biology.organism_classification ,Ligands ,Molecular biology ,Immunity, Innate ,Fish Diseases ,Vibrio Infections ,biology.protein ,Environmental Chemistry ,Animals ,Bass ,CCL25 ,Chemokines ,CC chemokine receptors ,Chemotaxis assay ,Vibrio - Abstract
CC motif chemokine ligand 25 (CCL25) is a key chemokine that attracts various types of leukocytes, such as activated peritoneal macrophages. However, information on CCL25 in fish is limited. Here, a CCL25 gene (LjCCL25) was identified from Japanese sea bass (Lateolabrax japonicus), showing upregulation in multiple tissues against Vibrio harveyi infection. The recombinant LjCCL25 (rLjCCL25) only significantly induced the migration of monocytes/macrophages (MO/MΦ) both in vitro and in vivo, but didn't induce that of neutrophils or lymphocytes. Additionally, rLjCCL25 only induced migration of the lipopolysaccharide-stimulated MO/MΦ (M1 type). Knockdown of Japanese sea bass CC chemokine receptor 9 (LjCCR9) expression in MO/MФ by RNA interference inhibited the LjCCL25-induced chemotaxis of resting and M1 type MO/MФ. Moreover, administration of 300 ng/g rLjCCL25 effectively increased the survival of V. harveyi-infected fish and decreased bacterial load. Our study demonstrates that LjCCL25 functions as an MO/MФ chemoattractant via LjCCR9 in Japanese sea bass against V. harveyi.
- Published
- 2021
26. Development of Genomic SSR for the Subtropical Hardwood Tree Dalbergia hupeana and Assessment of Their Transferability to Other Related Species
- Author
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Yu Liu, Ping Huang, Chang-Hong Li, Yongqi Zheng, and Furong Lin
- Subjects
0106 biological sciences ,Germplasm ,0303 health sciences ,Genetic diversity ,biology ,Contig ,Forestry ,genetic diversity ,genomic SSRs ,biology.organism_classification ,01 natural sciences ,Genome ,DNA sequencing ,Dalbergia hupeana ,03 medical and health sciences ,Dalbergia ,Evolutionary biology ,genome survey ,Genetic variation ,cross-species transferability ,QK900-989 ,Plant ecology ,Genome size ,030304 developmental biology ,010606 plant biology & botany - Abstract
Dalbergia hupeana Hance (D. hupeana) is a precious hardwood tree of the genus Dalbergia. It is one of the few species widely distributed within subtropical areas and is important for timber production and forest restoration. At present, there is little published genetic information on D. hupeana. Therefore, we performed a genome survey using next generation sequencing (NGS) and developed a set of novel genomic SSR (gSSR) markers from the assembled data, and assessed the transferability of these markers to other Dalbergia species in Asia. The results of the genome survey show the genome size of D. hupeana to be about 664 Mb and highly heterozygous. The assembly of sequencing data produced 2,431,997 contigs, and the initial assembly of the NGS data alone resulted in contig N50 of 393 kb with a total of 720 Mb. A total of 127,742 perfect SSR markers were found in the assembled contigs. A total of 37 highly polymorphic and easily genotyped gSSR markers were developed in D. hupeana, while the majority of gSSR markers could be successfully transferred to nine other Dalbergia species in Asia. The transferability rate of gSSR markers was highest in D. balansae, which is more closely related to D. hupeana. Seven gSSR markers were able to be amplified in all tested species. In addition, a preliminary assessment of the genetic diversity of three tree species in the Dalbergia genus suggested a high level of genetic diversity within populations distributed in the subtropical area in China. However, the determination of the global status of their genetic variation still requires further and more comprehensive assessment. Our findings will enable further studies on the genetic diversity, phylogenetics, germplasm characterization, and taxonomy of various Dalbergia species.
- Published
- 2021
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27. Safety assessment of MPTA: An oral acute and 90-day sub-chronic toxicity study in Sprague-Dawley rats
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Zhen Dong, Shu-sheng Tang, Chang-hong Li, Zhao-shan Tang, Zi-hui Yang, and Jian-guo Zeng
- Subjects
Lethal Dose 50 ,Male ,Quaternary Ammonium Compounds ,Rats, Sprague-Dawley ,Plant Extracts ,Toxicity Tests, Subchronic ,Toxicity Tests, Acute ,Administration, Oral ,Animals ,Female ,General Medicine ,Toxicology ,Rats - Abstract
MPTA is a novel extract product derived from Macleaya cordata (Willd.) R. Br., which has good anti-inflammatory and antioxidant activity. The aim of this study was to investigate the acute oral toxicity and 90-day sub-chronic oral toxicity of MPTA. In the acute toxicity study, 50 SD rats of both sexes were randomly divided into 5 groups and dosed in a gradient from 197.53 mg/kg to 1000.00 mg/kg bw. Toxic effects were observed up to 14 days and LD
- Published
- 2022
28. Floral development of Kingdonia (Ranunculaceae s. l., Ranunculales)
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Ren, Yi, Li, Zhi-jun, Chang, Hong-li, Lei, Yong-ji, and Lu, An-min
- Published
- 2004
29. Metal-size influence in isoselective 2-vinylpyridine polymerization
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Chang-Hong Li, Tieqi Xu, and Zhi-Qi Yu
- Subjects
chemistry.chemical_classification ,2-Vinylpyridine ,010405 organic chemistry ,Ether ,Polymer ,010402 general chemistry ,01 natural sciences ,Toluene ,0104 chemical sciences ,Catalysis ,Inorganic Chemistry ,chemistry.chemical_compound ,chemistry ,Polymerization ,Tacticity ,Polymer chemistry ,Materials Chemistry ,Physical and Theoretical Chemistry ,Selectivity - Abstract
Eight new organometallic complexes were obtained by the reaction of trialkyl rare earth complexes Ln(CH2SiMe3)3(THF)2 (Ln = Y, Lu, Ho, Dy) with tetradentate phenol ether ligands. Among them the structure of complex 7 with Lu was confirmed by single crystal X-ray diffraction. These complexes exhibited moderate to high activity for 2-VP polymerization at ambient temperature in toluene. Complex 5 showed the highest catalytic activity, with a turnover frequency of 1714 h−1. Significantly, the 2-VP polymerization is also stereospecific, producing isotactic poly(2-VP) with an isotacticity of mm = 0.44–0.99. The catalytic experimental results indicate that the size of metal atom obviously influences the stereoregularity of the growing polymer chain, and catalysts with Ho atom exhibit better isotactic selectivity than other metal catalysts.
- Published
- 2019
30. A novel CC chemokine ligand 2 like gene from ayu Plecoglossus altivelis is involved in the innate immune response against to Vibrio anguillarum
- Author
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Chang-Hong Li, Li Yu, and Jiong Chen
- Subjects
Fish Proteins ,0301 basic medicine ,Vibrio anguillarum ,Chemokine ,Aquatic Science ,Biology ,Proinflammatory cytokine ,Fish Diseases ,03 medical and health sciences ,Immune system ,Phagocytosis ,Animals ,Environmental Chemistry ,Amino Acid Sequence ,Chemokine CCL2 ,Phylogeny ,Innate immune system ,Gene Expression Profiling ,Chemotaxis ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Molecular biology ,Immunity, Innate ,Respiratory burst ,030104 developmental biology ,Gene Expression Regulation ,Osmeriformes ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,Plecoglossus altivelis ,Sequence Alignment - Abstract
Chemokine (CC motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein 1 (MCP-1), is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages (MO/MФ) in mammals. However, the functional repertoire of fish CCL2 remains unclear. Here, we identified a cDNA sequence encoding a novel CCL2-like protein (PaCCL2L) in ayu, Plecoglossus altivelis. Sequence analysis revealed that PaCCL2L grouped with CCL2 homologs, and is most closely related to Mexican tetra (Astyanax mexicanus) and zebrafish (Danio rerio) homologs. PaCCL2 transcripts were expressed in all tested tissues from healthy ayu, with the highest level in the spleen. Upon Vibrio anguillarum infection, PaCCL2L transcripts increased significantly in tested tissues, including the liver, spleen, and head kidney. We then produced the recombinant PaCCL2L mature peptide (rPaCCL2L) by prokaryotic expression and generated the corresponding antibodies (anti-PaCCL2L). A significant increase in PaCCL2L protein and mRNA expression was observed in ayu MO/MФ following V. anguillarum challenge. Intraperitoneal injection of rPaCCL2L resulted in significantly improved survival and reduced tissue bacterial load in V. anguillarum-infected ayu. rPaCCL2L had a positive effect on the chemotaxis of MO/MΦ and neutrophils both in vitro and in vivo. Meanwhile, rPaCCL2L exhibited a positive effect on the chemotaxis of LPS-stimulated MO/MΦ (M1 type) in vitro, whereas it exhibited no chemotaxis effect on cAMP-stimulated MO/MΦ (M2 type). In addition, rPaCCL2L treatment exhibited an enhanced effect on MO/MΦ phagocytosis, bacterial killing, respiratory burst, and mRNA expression of proinflammatory cytokines, whereas anti-PaCCL2L treatment had an inhibitory effect. Our study demonstrates that PaCCL2L might play a role in the immune response of ayu against V. anguillarum infection through chemotactic recruitment and activation of MO/MΦ.
- Published
- 2019
31. Characterization of the complete chloroplast genome of
- Author
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Yu, Liu, Ping, Huang, Chang-Hong, Li, Feng-Qi, Zang, and Yong-Qi, Zheng
- Subjects
Dalbergia cultrata ,phylogenetic analysis ,chloroplast genome ,Mitogenome Announcement ,Research Article - Abstract
Dalbergia cultrata is a Near Threatened species with high ecological and economic values. In this study, its chloroplast genome was assembled using Illumina pair-end sequencing dataset. The chloroplast genome has a quadripartite structure with 156,385 bp in length and contains a pair of 16,392 bp inverted repeat (IR) regions, which were separated by large single copy (LSC: 86,040 bp) region and small single copy (SSC: 37,561 bp) region. A total of 121 genes were annotated, including 77 protein-coding genes (PCGs), 36 tRNAs, and 8 rRNAs. The overall GC content was 36.1%. The phylogenetic analysis revealed that D. cultrata has close relationship to D. hainanensis and D. odorifera. This complete chloroplast genome can be readily used for population genetic studies of D. cultrata.
- Published
- 2020
32. High glutamate concentration reverses the inhibitory effect of microglial adenosine 2A receptor on NLRP3 inflammasome assembly and activation
- Author
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Hao Du, Yan Tan, Chang-Hong Li, Yan Zhao, Ping Li, Ya-Lei Ning, Ruo-Bing Gao, Bo Wang, Yan Peng, Si-Wei Tan, Zhi-Zhong Huang, Xing Chen, Nan Yang, Fa-Bo Shan, Ren-Ping Xiong, and Yuan-Guo Zhou
- Subjects
Mice ,Receptor, Adenosine A2A ,Inflammasomes ,General Neuroscience ,NLR Family, Pyrin Domain-Containing 3 Protein ,Animals ,Glutamic Acid ,Microglia ,Protein Multimerization ,Cells, Cultured - Abstract
NLRP3 inflammasome plays a crucial role in the innate immune system. Our group previously reported that the microglial adenosine 2A receptor (A
- Published
- 2022
33. Molecular markers from the chloroplast genome of rose provide a complementary tool for variety discrimination and profiling
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Chang-Hong Li, Yongqi Zheng, and Ping Huang
- Subjects
0106 biological sciences ,0301 basic medicine ,Chloroplasts ,DNA, Plant ,Genotype ,Sequence analysis ,Pseudogene ,Rosa odorata ,Population ,lcsh:Medicine ,Biology ,Rosa ,01 natural sciences ,Genome ,Polymorphism, Single Nucleotide ,Article ,03 medical and health sciences ,symbols.namesake ,Genetics ,education ,lcsh:Science ,Genome, Chloroplast ,Gene ,Phylogeny ,Whole genome sequencing ,Sanger sequencing ,education.field_of_study ,Comparative Genomic Hybridization ,Multidisciplinary ,lcsh:R ,Electrophoresis, Capillary ,Sequence Analysis, DNA ,030104 developmental biology ,symbols ,lcsh:Q ,Plant sciences ,010606 plant biology & botany ,Microsatellite Repeats - Abstract
The rose is one of the most important ornamental woody plants because of its extensive use and high economic value. Herein, we sequenced a complete chloroplast genome of the miniature rose variety Rosa ‘Margo Koster’ and performed comparative analyses with sequences previously published for other species in the Rosaceae family. The chloroplast genome of Rosa ‘Margo Koster’, with a size of 157,395 bp, has a circular quadripartite structure typical of angiosperm chloroplast genomes and contains a total of 81 protein-coding genes, 30 tRNA genes and 4 rRNA genes. Conjunction regions in the chloroplast genome of Rosa ‘Margo Koster’ were verified and manually corrected by Sanger sequencing. Comparative genome analysis showed that the IR contraction and expansion events resulted in rps19 and ycf1 pseudogenes. The phylogenetic analysis within the Rosa genus showed that Rosa ‘Margo Koster’ is closer to Rosa odorata than to other Rosa species. Additionally, we identified and screened highly divergent sequences and cpSSRs and compared their power to discriminate rose varieties by Sanger sequencing and capillary electrophoresis. The results showed that 15 cpSSRs are polymorphic, but their discriminating power is only moderate among a set of rose varieties. However, more than 150 single nucleotide variations (SNVs) were discovered in the flanking region of cpSSRs, and the results indicated that these SNVs have a higher divergence and stronger power for profiling rose varieties. These findings suggest that nucleotide mutations in the chloroplast genome may be an effective and powerful tool for rose variety discrimination and DNA profiling. These molecular markers in the chloroplast genome sequence of Rosa spp. will facilitate population and phylogenetic studies and other related studies of this species.
- Published
- 2020
34. Immune and gut bacterial successions of large yellow croaker (Larimichthys crocea) during Pseudomonas plecoglossicida infection
- Author
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Chang-Hong Li, Jiong Chen, Jinbo Xiong, and Fei-Fei Ding
- Subjects
0301 basic medicine ,medicine.medical_treatment ,Aquaculture ,Aquatic Science ,Gut flora ,digestive system ,Microbiology ,03 medical and health sciences ,Fish Diseases ,Immune system ,Pseudomonas ,medicine ,Environmental Chemistry ,Larimichthys crocea ,Animals ,Pseudomonas Infections ,Pathogen ,Pseudomonas plecoglossicida ,biology ,Host Microbial Interactions ,Host (biology) ,04 agricultural and veterinary sciences ,General Medicine ,medicine.disease ,biology.organism_classification ,Gastrointestinal Microbiome ,Perciformes ,030104 developmental biology ,Cytokine ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Cytokines ,Dysbiosis - Abstract
Large yellow croaker (Larimichthys crocea, LYC) aquaculture is being threatened by intensive infectious diseases. Relevant studies have focused on LYC immune responses to infection. By contrast, little is known how and to what extent the gut microbiota responds to infection. Here, we explored the interactions between LYC immune responses and gut bacterial communities during Pseudomonas plecoglossicida infection. P. plecoglossicida successfully colonized into LYC gut microbiota, resulting in an increasing mortality rate. Relative gene expressions of pro-inflammatory cytokines (TNF-α1, TNF-α2 and IL-1β) and anti-inflammatory cytokine (IL-10) were consistently and significantly induced by P. plecoglossicida infection, whereas non-specific immune enzymes activities were only enhanced at the early infection stages. P. plecoglossicida infection caused an irreversible disruption in the gut microbiota, of which infection and hours post infection constrained 16.2% and 5.6% variations, respectively. In addition, top 18 discriminatory taxa that were responsible for the difference between treatments were identified, whose abundances were significantly associated with the immune activities of LYC. Using a structural equation modeling (SEM), we found that gut bacterial communities were primarily governed by the conjointly direct (-0.33) and indirect (0) effects of infection, which subsequently affect host immune responses. Our results suggest that an irreversible dysbiosis in gut microbiota could be the causality of increasing mortality. To our knowledge, this is the first study to provide an integrated overview among pathogen infection, immune response and gut microbiota of LYC.
- Published
- 2019
35. Protective Effect of Norcantharidin on Collagen-Induced Arthritis Rats
- Author
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Qingqing Guo, Yun-jing Bai, Ze-jun Huo, Xiao-juan He, Chang-hong Li, Yukun Zhao, and Hong-bo Shen
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,Arthritis ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Pharmacology (medical) ,RNA, Messenger ,Norcantharidin ,business.industry ,Growth factor ,Interleukin ,Forkhead Transcription Factors ,General Medicine ,Nuclear Receptor Subfamily 1, Group F, Member 3 ,Bridged Bicyclo Compounds, Heterocyclic ,medicine.disease ,Arthritis, Experimental ,Vascular endothelial growth factor ,030104 developmental biology ,Cytokine ,Endocrinology ,Complementary and alternative medicine ,chemistry ,Cytokines ,Joints ,Methotrexate ,Tumor necrosis factor alpha ,business ,medicine.drug - Abstract
To observe the effect of norcantharidin (NCTD) on collagen-induced arthritis (CIA) rats. Sixty Sprague-Dawley(SD) rats were randomly divided into 6 groups (n=10): normal group, CIA model group(model group), NCTD low-dose group [1.35 mg/(kg•d)], NCTD middle-dose group [2.7 mg/(kg•d)], NCTD high-dose group [5.4 mg/(kg•d)] and methotrexate (MTX) group [1.8 mg/(kg/w)]. Anesthetized rats were sacrificed by luxation of cervical vertebra after 4 weeks of administration. The arthritis scores were evaluated twice a week. The pathological changes in the ankle joints of rats were observed by hematoxylin-eosin (H&E) staining. The serum levels of interleukin (IL) 1β, IL-6, tumor necrosis factor (TNF)-α, vascular endothelial growth factor (VEGF), IL-17 and transform growth factor (TGF) β were detected by enzyme linked immunosorbent assay (ELISA). The mRNA expression of retinoid-related orphan nuclear receptorγt (RORγt) and forkhead box P3 (Foxp3) in peripheral blood lymphocytes were confirmed by real-time polymerase chain reaction. MTX and high-dose NCTD not only decreased the arthritis scores but also alleviated the pathological changes in CIA rats’ ankle joints compared with the model group (P
- Published
- 2017
36. Two new isomeric zinc(II) metal–organic frameworks based on 1,5-bis(2-methyl-1H-imidazol-1-yl)pentane and 5-methylisophthalate ligands
- Author
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Chang Hong Li, Xiong Wen Tan, and Hengfeng Li
- Subjects
Thermogravimetric analysis ,010405 organic chemistry ,Inorganic chemistry ,Infrared spectroscopy ,chemistry.chemical_element ,Crystal structure ,Zinc ,010402 general chemistry ,Condensed Matter Physics ,01 natural sciences ,0104 chemical sciences ,Inorganic Chemistry ,Metal ,Pentane ,Solvent ,chemistry.chemical_compound ,chemistry ,visual_art ,Polymer chemistry ,Materials Chemistry ,visual_art.visual_art_medium ,Metal-organic framework ,Physical and Theoretical Chemistry - Abstract
Many factors, such as temperature, solvent, the central metal atom and the type of coligands, may affect the nature of metal–organic frameworks (MOFs) and the framework formation in the self-assembly process, which results in the complexity of these compounds and the uncertainty of their structures. Two new isomeric ZnIImetal–organic frameworks (MOFs) based on mixed ligands, namely, poly[[μ-1,5-bis(2-methyl-1H-imidazol-1-yl)pentane-κ2N3:N3′](μ-5-methylisophthalato-κ2O1:O3)zinc(II)], [Zn(C9H6O4)(C13H20N4)]n, (I), and poly[[μ-1,5-bis(2-methyl-1H-imidazol-1-yl)pentane-κ2N3:N3′](μ3-5-methylisophthalato-κ3O1:O1′:O3)(μ3-5-methylisophthalato-κ4O1:O1′:O3,O3′)dizinc(II)], [Zn2(C9H6O4)2(C13H20N4)]n, (II), have been synthesized under hydrothermal conditions and characterized by single-crystal X-ray diffraction, IR spectroscopy, elemental analysis and thermogravimetric analysis. Complex (I) displays a two-dimensional layer net, while complex (II) exhibits a twofold interpenetrating three-dimensional framework. Both complexes show high stability and good fluorescence in the solid state at room temperature.
- Published
- 2017
37. Characterization of the complete chloroplast genome of Liquidambar acalycina Chang
- Author
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Yongqi Zheng, Ping Huang, Jiu-Xin Lai, Chang-Hong Li, and Furong Lin
- Subjects
0106 biological sciences ,0301 basic medicine ,Biology ,010603 evolutionary biology ,01 natural sciences ,Genome ,Chloroplast ,03 medical and health sciences ,030104 developmental biology ,Botany ,Ornamental plant ,Genetics ,Liquidambar acalycina ,Molecular Biology ,Tree species - Abstract
Liquidambar acalycina is a popular tree species with high ornamental and economic values because of the leaf shape and coloration. In this study, chloroplast genome of L.acalycina was assembled usi...
- Published
- 2020
38. Effect of oxidative stress injury on miR-210 expression in spinal neurons and the neuroprotective effect of miR-210 inhibitor
- Author
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Yong-Jin He, Chang-Hong Li, and Zhi-Bin Liu
- Subjects
nervous system ,Oxidative stress injury ,lcsh:R ,lcsh:Medicine ,Apoptosis ,Spinal cord injury ,Neuron ,miR-210 - Abstract
Objective: To study the effect of oxidative stress injury on miR-210 expression in spinal neurons and the neuroprotective effect of miR-210 inhibitor. Methods: Spinal neurons of rats were cultured and divided into control group (group A), H2O2 group (group B), NC group (group C), H2O2+NC group (group D) and H2O2+miR-210 inhibitor group (group E), miR-210 expression levels in group A and group B as well as the cell vitality and expression levels of oxidative stress molecules, apoptotic molecules and function-related molecules in group C, group D and group E were determined. Results: miR-210 expression level in group B was significantly higher than that in group A; after 12 h, 18 h and 24 h of treatment, OD values of group D were significantly lower than those of group C, and OD values of group E were significantly higher than those of group D; after 24 h of treatment, HO-1, NOX-2, CAT, GAP-43 and synapsin-I levels in group D were significantly lower than those in group C while ROS, 8-OHdG, c-fos, c-jun, AP1, caspase-3, PSD-93 and PSD-95 levels were significantly higher than those in group C; HO-1, NOX-2, CAT, GAP-43 and synapsin-I levels in group E were significantly higher than those in group D while ROS, 8-OHdG, c-fos, c-jun, AP1, caspase-3,PSD-93 and PSD-95 levels were significantly lower than those in group D. Conclusion: Oxidative stress injury can cause high miR-210 expression in spinal neurons, and miR-210 inhibitor can reduce the neuron injury caused by oxidative stress.
- Published
- 2016
39. Molecular characterization of an interleukin-4/13B homolog in grass carp ( Ctenopharyngodon idella ) and its role in fish against Aeromonas hydrophila infection
- Author
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Ming-Yun Li, Chang-Hong Li, Jie Chen, Hao Zhang, Zhi-Jing Yang, and Jiong Chen
- Subjects
Fish Proteins ,0301 basic medicine ,Carps ,DNA, Complementary ,Phagocytosis ,Aquatic Science ,Cyprinus ,Microbiology ,Proinflammatory cytokine ,Fish Diseases ,03 medical and health sciences ,Common carp ,0302 clinical medicine ,Western blot ,medicine ,Animals ,Environmental Chemistry ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Phylogeny ,Interleukin 4 ,biology ,medicine.diagnostic_test ,General Medicine ,biology.organism_classification ,Bacterial Load ,Immunity, Innate ,Recombinant Proteins ,Aeromonas hydrophila ,Grass carp ,030104 developmental biology ,Interleukin-4 ,Gram-Negative Bacterial Infections ,Sequence Alignment ,030215 immunology - Abstract
Mammalian interleukin 4 (IL-4) and interleukin 13 (IL-13) molecules are anti-inflammatory cytokines mediating the alternative activation of macrophages. However, the role of fish IL-4/13 homologs in monocytes/macrophages (MO/MФ) polarization remains unclear. In this study, we have functionally identified an IL-4/13B homolog in grass carp (Ctenopharyngodon idella), which is termed as CiIL-4/13B. Multiple alignment showed that CiIL-4/13B shared the typical characteristics and structure with other known fish IL-4/13. Phylogenetic analysis showed that CiIL-4/13B is evolutionarily closely related to zebrafish (Danio rerio) and common carp (Cyprinus carpio) IL-4/13B. CiIL-4/13B mRNA was constitutively expressed in tissues and peripheral blood lymphocytes (PBLs) examined, with its highest expression seen in PBLs. Following Aeromonas hydrophila infection, CiIL-4/13B mRNA expression was upregulated. Recombinant CiIL-4/13B (rCiIL-4/13B) was overexpressed in Escherichia coli and purified for a functional study. Using prepared anti-rCiIL-4/13B antiserum, Western blot analysis showed that native CiIL-4/13B in grass carp plasma is N-glycosylated. Intraperitoneal injection of bioactive rCiIL-4/13B significantly increased the survival rate of grass carp against A. hydrophila, and decreased the tissue bacterial load, with a higher dose having better effects. Bioactive rCiIL-4/13B treatment decreased nitrite production and mRNA expression of proinflammatory cytokines (IL-1β and TNF-α), while it increased arginase activity and mRNA expression of anti-inflammatory cytokines (TGF-β and IL-10). The phagocytosis by grass carp MO/MФ had no significant changes by the 8 h treatment of bioactive rCiIL-4/13B compared to that of the negative control, while it was significantly inhibited by the 24 h treatment of bioactive rCiIL-4/13B. The inhibitory effect of rCiIL-4/13B on MO/MФ phagocytosis may be a consequence of MO/MФ proliferation. In summary, our results suggest that CiIL-4/13B plays a protective effect in grass carp against A. hydrophila by inducing alternatively activated MO/MФ.
- Published
- 2016
40. Interaction of fish oil and a glucocorticoid on metabolic responses to an oral glucose load in healthy human subjects
- Author
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Delarue, Jacques, Chang-Hong, Li, Richard, Cohen, Charlotte, Corporeau, and Brigitte, Simon
- Published
- 2006
41. Cathelicidin modulates the function of monocytes/macrophages via the P2X7 receptor in a teleost, Plecoglossus altivelis
- Author
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Xin-Jiang Lu, Ming-Yun Li, Chang-Hong Li, and Jiong Chen
- Subjects
Fish Proteins ,medicine.medical_treatment ,Phagocytosis ,Antimicrobial peptides ,Aquatic Science ,Biology ,Monocytes ,Cathelicidin ,Immune system ,Western blot ,Cathelicidins ,medicine ,Animals ,Environmental Chemistry ,medicine.diagnostic_test ,Chemotaxis ,Macrophages ,Sequence Analysis, DNA ,General Medicine ,Molecular biology ,Respiratory burst ,Gene Expression Regulation ,Osmeriformes ,Receptors, Purinergic P2X7 ,Plecoglossus altivelis ,Antimicrobial Cationic Peptides - Abstract
Cathelicidins (CATHs) are a family of endogenous antimicrobial peptides that are capable of both direct bacteria-killing and immunomodulatory effects. P2X7 receptor (P2X7R) is a mediator of CATH in mammalian immune cells. Here, we studied the function and regulation of CATH in head kidney-derived monocytes/macrophages (MO/MФ) from ayu, Plecoglossus altivelis. We investigated the chemotaxis of MO/MФ in response to ayu CATH (PaCATH), and found that PaCATH had a dose-dependent effect on MO/MФ chemotaxis with the optimal concentration of 10.0 μg/ml. The qPCR and Western blot analysis revealed that PaCATH inhibited the expression of ayu P2X7R (PaP2X7R) at both mRNA and protein levels. Knockdown of the PaP2X7R expression in ayu MO/MФ by RNA interference not only significantly inhibited the chemotactic effect of PaCATH on MO/MФ, but also obviously reduced the effect of PaCATH on the phagocytosis, bacteria-killing, respiratory burst, and cytokine expression of ayu MO/MФ. Our study revealed that the immunomodulatory effect of fish CATH is mediated by P2X7R.
- Published
- 2015
42. [Untitled]
- Author
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Fei-Fei Ding, Jie Chen, Jiong Chen, Libin Mo, and Chang-Hong Li
- Subjects
0301 basic medicine ,Cloning ,MHC class II ,biology ,04 agricultural and veterinary sciences ,Aquatic Science ,Molecular biology ,03 medical and health sciences ,030104 developmental biology ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,Animal Science and Zoology ,Plecoglossus altivelis - Published
- 2018
43. Characterization of the complete chloroplast genome of Dalbergia cultrata (Leguminosae)
- Author
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Fengqi Zang, Yongqi Zheng, Ping Huang, Chang-Hong Li, and Yu Liu
- Subjects
0106 biological sciences ,0301 basic medicine ,Near-threatened species ,Biology ,010603 evolutionary biology ,01 natural sciences ,Genome ,Chloroplast ,03 medical and health sciences ,030104 developmental biology ,Botany ,Genetics ,Dalbergia cultrata ,Molecular Biology - Abstract
Dalbergia cultrata is a Near Threatened species with high ecological and economic values. In this study, its chloroplast genome was assembled using Illumina pair-end sequencing dataset. The chlorop...
- Published
- 2019
44. Molecular characterization of the liver-expressed antimicrobial peptide 2 (LEAP-2) in a teleost fish, Plecoglossus altivelis: Antimicrobial activity and molecular mechanism
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Chang-Hong Li, Xin-Jiang Lu, Jiong Chen, and He-Xiang Li
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Fish Proteins ,Vibrio anguillarum ,Interleukin-1beta ,Immunology ,Biology ,Liver-expressed antimicrobial peptide ,Microbiology ,Fish Diseases ,Immune system ,Complementary DNA ,Gene expression ,Animals ,RNA, Messenger ,Molecular Biology ,Vibrio ,Innate immune system ,Tumor Necrosis Factor-alpha ,Fishes ,biology.organism_classification ,Antimicrobial ,Molecular biology ,Gene Expression Regulation ,Liver ,Organ Specificity ,Vibrio Infections ,Plecoglossus altivelis ,Antimicrobial Cationic Peptides - Abstract
Liver-expressed antimicrobial peptide 2 (LEAP-2) is widespread in fish and plays an important role in the host's innate immune system. In this study, full-length cDNA for LEAP-2 (PaLEAP-2) gene was cloned and sequenced from ayu, Plecoglossus altivelis. PaLEAP-2 mRNA was detected in a wide range of tissues, with the highest level of transcripts found in the liver. Upon induction by Vibrio anguillarum, its expression significantly increased in the liver, kidney, spleen, gill, and heart, but decreased in the intestine. The PaLEAP-2 mature peptide was chemically synthesized; it exhibited selective antimicrobial activity against various bacteria in vitro. PaLEAP-2 at high concentration reduced the bacterial load and improved the survival rate of V. anguillarum-infected ayu. Moreover, it inhibited the expression of mRNAs for TNF-α and IL-1β in V. anguillarum-infected ayu, both at high and low concentrations. PaLEAP-2 induced hydrolysis of the pET-22b plasmid DNA and bacterium genomic DNA. These results suggest that PaLEAP-2 plays a role in ayu immune responses against bacterial infection.
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- 2015
45. Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection
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Jun WU, Yu-Hong SHI, Xue-Heng ZHANG, Chang-Hong LI, Ming-Yun LI, and Jiong CHEN
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Interleukin 1β ,Survival rate ,Large yellow croaker ,lcsh:Zoology ,lcsh:QL1-991 ,Vibrio alginolyticus ,Monocytes/ macrophages - Abstract
Interleukin 1β (IL-1β), the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the cDNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β (LcIL-1β) gene was most closely related to that of European seabass (Dicentrarchus labrax), sharing 67.8% amino acid identity. In healthy large yellow croaker, LcIL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, LcIL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant LcIL-1β (rLcIL-1β) improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, rLcIL-1β induced monocytes/macrophages (MO/MΦ) chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that LcIL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.
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- 2015
46. Crystal structure, magnetic, fluorescent, electrochemical properties and thermal stability of a new copper(II) coordination polymer [Cu2(C5H4NCOO)2(C7H5N4)2] n
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Hengfeng Li, Chang-Hong Li, Wei Li, and Ying-Qun Yang
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chemistry.chemical_compound ,chemistry ,Coordination polymer ,Inorganic chemistry ,chemistry.chemical_element ,Thermal stability ,General Chemistry ,Crystal structure ,Electrochemistry ,Copper ,Fluorescence - Abstract
A new copper(II) coordination polymer [Cu2(C5H4NCOO)2(C7H5N4)2] n (1) has been synthesized with 4-pyridinecarboxylate and 3-(pyridin-2-yl)-1,2,4-triazolyl ligands. The crystal structure shows that two neighboring copper(II) ions are coordinated with two deprotonated (anionic) 3-(pyridin-2-yl)-1,2,4-triazole ligands to form a binuclear structure. Adjacent binuclear units are linked by 4-pyridinecarboxylate anions to form a three-dimensional network structure. The magnetic, fluorescent, and electrochemical properties and thermal stability of 1 were studied. The results show that 1 exhibits antiferromagnetic interaction, and upon excitation at 318 nm, it has an intense fluorescent emission at approximately 430 nm. The electron transfer of 1 is irreversible in electrode reactions. The thermogravimetric analysis shows that 1 is stable below 240 °C.
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- 2015
47. Development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals
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De-Min Zhang, Chang-Hong Li, Xiaojun Yan, Jiong Chen, Yan-Jun Zhang, Yu-Hong Shi, Qian-Jin Zhou, Lei Wang, and Rui-Na Wang
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Microbiology (medical) ,Vibrio alginolyticus ,Aquatic Organisms ,Vibrio anguillarum ,Bacteria ,biology ,Vibrio harveyi ,Vibrio parahaemolyticus ,Microfluidics ,Loop-mediated isothermal amplification ,Pathogenic bacteria ,Vibrio vulnificus ,biology.organism_classification ,medicine.disease_cause ,Microbiology ,Vibrio fluvialis ,medicine ,Animals ,Seawater ,Nucleic Acid Amplification Techniques ,Molecular Biology - Abstract
Rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. In this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., Nocardia seriolae, Pseudomonas putida, Streptococcus iniae, Vibrio alginolyticus, Vibrio anguillarum, Vibrio fluvialis, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio rotiferianus, and Vibrio vulnificus. The assay provided a nearly-automated approach, with only a single pipetting step per chip for sample dispensing. This technique could achieve limits of detection (LOD) ranging from 0.40 to 6.42pg per 1.414μL reaction in less than 30 min. The robust reproducibility was demonstrated by a little variation among duplications for each bacterium with the coefficient of variation (CV) for time to positive (Tp) value less than 0.10. The clinical sensitivity and specificity of this on-chip LAMP assay in detecting field samples were 96.2% and 93.8% by comparison with conventional microbiological methods. Compared with other well-known techniques, on-chip LAMP assay provides low sample and reagent consumption, ease-of-use, accelerated analysis, multiple bacteria and on-site detection, and high reproducibility, indicating that such a technique would be applicable for on-site detection and routine monitoring of multiple pathogens in aquaculture.
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- 2014
48. Genomic insights into Aspergillus sydowii 29R-4-F02: unraveling adaptive mechanisms in subseafloor coal-bearing sediment environments
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Jun-Peng Jiang, Xuan Liu, Yi-Fan Liao, Jun Shan, Yu-Ping Zhu, and Chang-Hong Liu
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Aspergillus sydowii ,subseafloor sediments ,fungi ,CAZymes ,adaptive evolution ,Microbiology ,QR1-502 - Abstract
IntroductionAspergillussydowii is an important filamentous fungus that inhabits diverse environments. However, investigations on the biology and genetics of A. sydowii in subseafloor sediments remain limited.MethodsHere, we performed de novo sequencing and assembly of the A. sydowii 29R-4-F02 genome, an isolate obtained from approximately 2.4 km deep, 20-million-year-old coal-bearing sediments beneath the seafloor by employing the Nanopore sequencing platform.Results and DiscussionThe generated genome was 37.19 Mb with GC content of 50.05%. The final assembly consisted of 11 contigs with N50 of 4.6 Mb, encoding 12,488 putative genes. Notably, the subseafloor strain 29R-4-F02 showed a higher number of carbohydrate-active enzymes (CAZymes) and distinct genes related to vesicular fusion and autophagy compared to the terrestrial strain CBS593.65. Furthermore, 257 positively selected genes, including those involved in DNA repair and CAZymes were identified in subseafloor strain 29R-4-F02. These findings suggest that A. sydowii possesses a unique genetic repertoire enabling its survival in the extreme subseafloor environments over tens of millions of years.
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- 2023
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49. Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
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María Ángeles Esteban, Chang Hong Li, Elena Chaves-Pozo, Alberto Cuesta, and Héctor Cordero
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0301 basic medicine ,Signal peptide ,Fish Proteins ,gilthead seabream ,food.ingredient ,Acuicultura ,marine sciences ,Fish farming ,Ontogeny ,Immunology ,Down-Regulation ,Biology ,Microbiology ,histology ,03 medical and health sciences ,Bass (fish) ,Fish Diseases ,food ,medicine ,Animals ,molecular biology ,Nodaviridae ,Centro Oceanográfico de Murcia ,Sea bass ,European sea bass ,Gene ,fish ,teleost ,Haptoglobins ,Haptoglobin ,LCDV ,04 agricultural and veterinary sciences ,Anatomy ,Trypsin ,Sea Bream ,Up-Regulation ,030104 developmental biology ,Virus Diseases ,nodavirus ,040102 fisheries ,biology.protein ,cytology ,0401 agriculture, forestry, and fisheries ,Bass ,Haptoglobin, nodavirus, LCDV, teleost, gilthead seabream, European sea bass ,Developmental Biology ,medicine.drug - Abstract
Haptoglobin (Hp) molecule has been cloned and characterized in two marine teleosts (gilthead seabream and European sea bass), obtaining putative proteins of 319 residues encoded by an ORF of 960 bp in both species. However, the matrix of similarity revealed low identities among bony fish species 78.9% (seabream-sea bass), 43% (seabream/seabass-zebrafish) and lower than 20% with sharks and human. The protein sequences showed a signal peptide from the position 1 to 23, a trypsin domain from 47 to 297, and several predicted disulfide bridges and glycosylation sites. The expression of hp transcript levels during ontogeny showed a progressive increase of expression in seabream whilst remained almost unaltered in sea bass. By tissues, this gene was found constitutively expressed with the highest levels on liver in both species. The main results on hp transcript levels showed the up-regulation in gilthead seabream suffering from naturally occurring lymphocystis disease; and the down-regulation and up-regulation after nodavirus infection in the resistant gilthead seabream and the susceptible European sea bass, respectively. These findings demonstrate for the first time an important role of haptoglobin against viral infections, operating differently in two of the most important marine farmed fish species., SI
- Published
- 2017
50. Molecular characterization and functional analysis of two distinct liver-expressed antimicrobial peptide 2 (LEAP-2) genes in large yellow croaker (Larimichthys crocea)
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Jiong Chen, He-Xiang Li, Xin-Jiang Lu, and Chang-Hong Li
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Fish Proteins ,Molecular Sequence Data ,Spleen ,Aquatic Science ,Liver-expressed antimicrobial peptide ,Exon ,Hepcidins ,medicine ,Animals ,Environmental Chemistry ,Larimichthys crocea ,Amino Acid Sequence ,RNA, Messenger ,Gene ,Phylogeny ,Vibrio alginolyticus ,Innate immune system ,biology ,Intron ,General Medicine ,biology.organism_classification ,Molecular biology ,Perciformes ,medicine.anatomical_structure ,Sequence Alignment - Abstract
Liver-expressed antimicrobial peptide 2 (LEAP-2) plays a vital role in the host innate immune system. In the present study, two LEAP-2 genes (LcLEAP-2A and LcLEAP-2C) from large yellow croaker (Larimichthys crocea) were cloned, both of which consist of 3 exons and 2 introns. The LcLEAP-2A transcripts were expressed in a wide range of tissues, with the highest mRNA levels found in the liver and intestine, while LcLEAP-2C transcripts showed obvious lower mRNA levels in all tested tissues compared to LcLEAP-2A. Upon infection by Vibrio alginolyticus, LcLEAP-2A transcripts were significantly up-regulated in liver, trunk kidney, spleen, head kidney, and gill, but down-regulated in intestine. In addition, significant up-regulation of LcLEAP-2C transcripts were also detected in all tissues tested, including intestine. The LcLEAP-2A and LcLEAP-2C mature peptides were chemically synthesized and found to exhibit selective antimicrobial activity in vitro against various species of bacteria. LcLEAP-2C, but not LcLEAP-2A, had antimicrobial activity against V. alginolyticus. Moreover, LcLEAP-2C treatment at low concentrations was evaluated and found to improve survival rate in V. alginolyticus-infected large yellow croaker, resulting in a decrease in bacterial load and expression of inflammatory cytokines. These results suggest that LcLEAP-2 isoforms play an important role in innate immunity by killing bacteria and inhibiting early inflammatory response in large yellow croaker.
- Published
- 2014
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