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A recombinant human wild-type p53-induced phosphatase (rWip1) was prepared to identify the substrate specificity of Wip1. rWip1 showed properties that were comparable to those of protein phosphatase 2Calpha in terms of affinity for Mg(super 2+), insensitivity to okadaic acid, and threonine dephosphorylation.

In this paper, we present a hybrid representation for the attachment-mode current existing on the surface of a coaxially fed rectangular microstrip patch antenna. The hybrid representation consists of a one- or two-term residue and an eigenfunction series which gives the attachment-mode current at all points on the patch surface, including the probe-to-patch junction. It is numerically demonstrated that the residue and eigenfunction series blend smoothly in the close vicinity of the Stokes region which includes the probe-to-patch junction. The Stokes region is a very narrow region over which the attachment-mode current changes rapidly. The residue and eigenfunction series are used outside and within this Stokes region, respectively. This hybrid representation can be used in either spectral- or space-domain techniques for a full-wave solution to the microstrip antenna problem. The residue series is obtained by using an equivalent contour integral representation of the infinite eigenfunction series, which subsequently reduces to the Watson transform under the assumption of a slightly lossy substrate. At or near the Stokes regions the residue series is inadequate, as it cannot model the rapidly varying junction currents there. Examination of the residue series shows that the attachment mode is a superposition of exponentially attenuated, leaky, surface-traveling modes. This hybrid representation is expected to provide the optimum trade-off between speed and accuracy for computations involving electrically large finite arrays.

On December 19, 2018, the U.S. Food and Drug Administration (FDA) granted approval to olaparib monotherapy for first‐line maintenance treatment of BRCA‐mutated (BRCAm) advanced ovarian cancer and, on May 8, 2020, expanded the indication of olaparib to include its use in combination with bevacizumab for first‐line maintenance treatment of homologous recombination deficient (HRD)–positive advanced ovarian cancer. Both these approvals were based on randomized, double‐blind, placebo‐controlled trials. Approval for olaparib monotherapy was based on the SOLO‐1 trial, comparing the efficacy of olaparib versus placebo in patients with BRCAm advanced ovarian, fallopian tube, or primary peritoneal cancer after surgical cytoreduction and first‐line platinum‐based chemotherapy. Two companion diagnostic (CDx) tests were approved with this indication: BRACAnalysis CDx, for germline BRCA1/2 alterations, and FoundationOne CDx, for BRCA1/2 alterations in tissue specimens. Approval for olaparib in combination with bevacizumab was based on the results of the PAOLA‐1 trial that compared olaparib with bevacizumab versus placebo plus bevacizumab in patients with advanced high‐grade epithelial ovarian cancer, fallopian tube, or primary peritoneal cancer after first‐line platinum‐based chemotherapy and bevacizumab. Myriad myChoice CDx was designated as a companion diagnostic device for use of olaparib plus bevacizumab combination for ovarian cancer associated with HRD‐positive status. Both trials demonstrated clinically meaningful improvements in progression‐free survival and favorable benefit‐risk profiles for the indicated populations. This article summarizes the FDA thought process and data supporting the approval of olaparib as monotherapy and in combination with bevacizumab for maintenance therapy in this setting. Implications for Practice: These approvals represent the first poly (ADP‐ribose) polymerase inhibitor, alone or in combination with bevacizumab, approved in first‐line maintenance treatment of women with advanced ovarian cancer after cytoreductive surgery and chemotherapy. In patients with BRCA‐mutated tumors, olaparib monotherapy demonstrated a 70% reduction in the risk of disease progression or death compared with placebo, and olaparib in combination with bevacizumab demonstrated a 67% reduction in the risk of disease progression or death compared with bevacizumab alone in homologous recombination deficient–positive tumors. These approvals represent a major advance for the treatment of women with advanced ovarian cancer who are in complete or partial response after their initial platinum‐based chemotherapy. Despite the best standard of care for newly diagnosed advanced ovarian cancer, approximately 70% of women relapse within the first 3 years. This article presents the FDA's rationale for approval of olaparib for the maintenance therapy of patients with deleterious or suspected deleterious germline or somatic BRCA‐mutated advanced epithelial ovarian, fallopian tube, or primary peritoneal cancer who are in complete or partial response to first‐line platinum‐based chemotherapy. [ABSTRACT FROM AUTHOR]

In this paper we examine the effect of truncating an infinite asymptotic series for the Hankel function used in microstrip antenna calculations. In particular, the accuracy of this truncated asymptotic expansion of the Hankel function is examined from a numerical viewpoint. This expansion has been used in the literature to obtain closed-form expressions for the microstrip Green's function for subsequent use in calculating mutual coupling between elements in a microstrip array. In this paper, we show that truncating the asymptotic series for the Hankel function could lead to severe unexpected errors for those values of the argument where the asymptotic expansion is normally expected to be valid. This is known as the Stokes phenomenon and has generally remained obscure in the literature. Since the large argument of the Hankel function is shown to be related to the lateral separation between two antennas, the results presented here have a particular bearing in calculating mutual coupling between widely separated elements in electrically large microstrip arrays.

Studies of a recombinant human Wip1 catalytic domain (rWip1) conducted by measuring the kinetic parameters for variants of the ATM(1981pS) phosphopeptide, AFEEGpSQSTTI is discussed. The findings from the studies could be useful to regulate physiological substrates by Wip1 and to develop selective anticancer drugs.

Five HIV-1 reverse transcriptase variants containing tyrosine analogues at position 115 of their p66 subunit are evaluated. The findings are discussed within the context of detailed structural models of HIV-1 reverse transcriptase ternary complexes containing this amino acid substitution that reveal probable mechanisms of the observed catalytic rate changes.