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Electrical injury is a relatively uncommon but potentially devastating form of multi-system injury with high morbidity and mortality. In common electric injury cases, it is usually difficult to find characteristic changes of electric injury in major organs by using routine histopathological test methods unless there are landmark traces of electric injury, known as electric marks. How to determine electric shock death, especially in the absence of typical electrical marks on the body surface in some cases (which account for about two-thirds of electric injury cases), remains a challenging problem in forensic practice. Our summary shows that many current related studies have focused their efforts to find characteristic histopathological changes in major organs of the body caused by electric injury. Based on the results obtained through comparison of the literature, we find that it may be more urgent and important to find the optimal autopsy or sampling sites in cases with no typical electric marks, knowing that these sites may often reflect the most significant histopathological changes of electric injury, for instance anatomy and sampling of the anterior wrist and the medial malleolus in cases involving the hand-to-foot electric circuit pathway. In this article, we make a summary of advances in identification methods of electric injury, hoping that it could provide some new insights for further research in this field. [ABSTRACT FROM AUTHOR]

It has been demonstrated that S1P receptors affect heart ischaemia–reperfusion (IR) induced injury. However, whether S1P receptors affect IR-induced cardiac death has not been investigated. The aim of this paper is to demonstrate the role of S1P receptors in IR-induced cardiac death. Healthy adult male Sprague–Dawley rats were assigned to the following groups: non-operation control group, sham operation group, IR group, IR group pretreated with DMSO, IR group pretreated with S1P3 agonist, IR group pretreated with an antagonist of S1P3, IR group pretreated with S1P2 and S1P3 antagonists, IR group pretreated with heptanol and antagonists of S1P2/3, and IR group pretreated with Gap26 and antagonists of S1P2/3 (heptanol acts as a Cx43 uncoupler and the mimic peptide Gap26 as Cx43 blocker). The groups with S1P2 or S1P3 agonist application before reperfusion were used to assess whether these can be used for therapy of IR. The haemodynamics, electrocardiograms (ECG), infarction area, and mortality rates were recorded. Immunohistological connexin 43 (Cx43) expression in the heart was detected in each group. Blocking S1P2/3 receptors with specific antagonists resulted in an increment of IR-induced mortality, increased infarction size, redistribution of Cx43 expression, as well as affecting the heart function. The infarction size, heart function, and mortality were totally or partially restored in the S1P2, S1P3 agonist-pretreated IR group, and the heptanol/Gap26-treated S1P2/3-blocked IR group. The S1P receptor S1P2/3 and Cx43 are involved in the IR-induced cardiac death. [ABSTRACT FROM AUTHOR]

Allicin has been reported to play a biological role in human pathophysiological processes via interaction with numerous signaling pathways and gene expression alteration. The purpose of the present study was to evaluate the protective effects of allicin against renal ischemia/reperfusion injury (RIRI) in rats. In the present study, the RIRI model with 45-min ischemia and 22-h reperfusion in rats was generated and allicin was used as the intervention. Changes in renal tissue pathomorphology, renal function, oxidative stress, inflammatory response and apoptosis were evaluated in the RIRI model in rats. Compared with those in the RIRI group, renal function, renal pathological injury, and anti-inflammatory and antioxidant properties were markedly improved in the RIRI+allicin group. Thus, our research suggested that allicin exerted its protective effect against ischemia/reperfusion-induced renal injury by regulating apoptosis, oxidative stress and inflammatory response in rats. [Display omitted] • Pretreatment with allicin could regulate renal function impairment induced by ischemia reperfusion. • Preconditioning with allicin participated in oxidative stress of RIRI. • Allicin played a vital role in RIRI by exerting anti-apoptotic and anti-inflammatory activities. [ABSTRACT FROM AUTHOR]

Semantic segmentation plays a pivotal role in the intelligent interpretation of remote sensing images (RSIs). However, conventional methods predominantly focus on learning representations within the spatial domain, often resulting in suboptimal discriminative capabilities. Given the intrinsic spectral characteristics of RSIs, it becomes imperative to enhance the discriminative potential of these representations by integrating spectral context alongside spatial information. In this paper, we introduce the spectrum-space collaborative network (SSCNet), which is designed to capture both spectral and spatial dependencies, thereby elevating the quality of semantic segmentation in RSIs. Our innovative approach features a joint spectral–spatial attention module (JSSA) that concurrently employs spectral attention (SpeA) and spatial attention (SpaA). Instead of feature-level aggregation, we propose the fusion of attention maps to gather spectral and spatial contexts from their respective branches. Within SpeA, we calculate the position-wise spectral similarity using the complex spectral Euclidean distance (CSED) of the real and imaginary components of projected feature maps in the frequency domain. To comprehensively calculate both spectral and spatial losses, we introduce edge loss, Dice loss, and cross-entropy loss, subsequently merging them with appropriate weighting. Extensive experiments on the ISPRS Potsdam and LoveDA datasets underscore SSCNet's superior performance compared with several state-of-the-art methods. Furthermore, an ablation study confirms the efficacy of SpeA. [ABSTRACT FROM AUTHOR]

The main cause of chronic renal allograft dysfunction (CRAD) still remains unclear. Insulin resistance (IR) may be a potential inducement, but there is insufficient evidence about this association. We aimed to establish a rat model of CRAD complicated with IR and to explore the function and pathologic changes of the renal allograft induced by IR. F344-to-Lewis rats of CRAD were fed a high-fat diet to induce IR. They were divided into 3 groups: IR (CRAD+IR), CRAD, and control (CTL). Serum levels of blood urea nitrogen (BUN) and serum creatinine (Scr) were measured to evaluate the renal function. The Homeostasis Model Assessment (HOMA)-IR index was detected by comparing the values of fasting serum insulin levels (FINS) with fasting blood glucose levels (FBG). The pathologic analysis was conducted by the degree of renal lesions including glomerular lesions, renal tubular lesions, hemorrhage, inflammatory cell infiltration, fibrillation, and hyperplasia of the renal interstitium. In the second, third, and fourth month after surgery, serum levels of Scr and BUN in the IR group were reduced more than those in the CRAD group, while they were both higher compared to the CTL group, suggesting that renal function in the CRAD group was declined. The HOMA-IR in the IR group was greater than that in the CRAD and CTL groups, showing that simple high-fat diet feeding significantly and steadily increased FINS and FBG in CRAD complicated with IR rats. Pathologic changes indicated that the CRAD rat model was successfully constructed and was still in the early-middle stages of renal lesions 4 months after surgery, yet IR presented a significant effect on CRAD. These results indicate that the stable CRAD complicated with IR rat model can be established through a high-fat diet in CRAD rats in 4 months, and IR could be an influencing factor. • Stable CRAD complicated with IR rat models can be established via a high-fat diet, in which IR could be an influencing factor. • Simple high-fat diet feeding steadily increased FINS and FBG in rats with CRAD complicated with IR. • HOMA-IR in the IR group was greater than that in the CRAD and CTL groups. [ABSTRACT FROM AUTHOR]

China is frequently subjected to local and regional drought disasters, and thus, drought monitoring is vital. Drought assessments based on available surface soil moisture (SM) can account for soil water deficit directly. Microwave remote sensing techniques enable the estimation of global SM with a high temporal resolution. At present, the evaluation of Soil Moisture Active Passive (SMAP) SM products is inadequate, and L-band microwave data have not been applied to agricultural drought monitoring throughout China. In this study, first, we provide a pivotal evaluation of the SMAP L3 radiometer-derived SM product using in situ observation data throughout China, to assist in subsequent drought assessment, and then the SMAP-Derived Soil Water Deficit Index (SWDI-SMAP) is compared with the atmospheric water deficit (AWD) and vegetation health index (VHI). It is found that the SMAP can obtain SM with relatively high accuracy and the SWDI-SMAP has a good overall performance on drought monitoring. Relatively good performance of SWDI-SMAP is shown, except in some mountain regions; the SWDI-SMAP generally performs better in the north than in the south for less dry bias, although better performance of SMAP SM based on the R is shown in the south than in the north; differences between the SWDI-SMAP and VHI are mainly shown in areas without vegetation or those containing drought-resistant plants. In summary, the SWDI-SMAP shows great application potential in drought monitoring. [ABSTRACT FROM AUTHOR]

Unbalanced DNA mixture is still a difficult problem for forensic practice. DIP-STRs are useful markers for detection of minor DNA but they are not widespread in the human genome and having long amplicons. In this study, we proposed a novel type of genetic marker, termed DIP-SNP. DIP-SNP refers to the combination of INDEL and SNP in less than 300bp length of human genome. The multiplex PCR and SNaPshot assay were established for 14 DIP-SNP markers in a Chinese Han population from Shanxi, China. This novel compound marker allows detection of the minor DNA contributor with sensitivity from 1:50 to 1:1000 in a DNA mixture of any gender with 1 ng–10 ng DNA template. Most of the DIP-SNP markers had a relatively high probability of informative alleles with an average I value of 0.33. In all, we proposed DIP-SNP as a novel kind of genetic marker for detection of minor contributor from unbalanced DNA mixture and established the detection method by associating the multiplex PCR and SNaPshot assay. DIP-SNP polymorphisms are promising markers for forensic or clinical mixture examination because they are shorter, widespread and higher sensitive. [ABSTRACT FROM AUTHOR]

Associations between gene variations and sudden cardiac arrest or coronary artery disease have been reported by genome-wide association studies. However, the implication of the genetic status in cases of sudden coronary death (SCD) from the Chinese Han population has remained to be investigated. The present study established a mini-sequencing system to examine putative death-causing single nucleotide polymorphisms (SNPs) using multiplex PCR, single base extension reaction and capillary electrophoresis techniques. A total of 198 samples from the Chinese Han population (age range, 34-71 years; mean age, 53.86 years) were examined using this method. Samples were classified into three groups: Coronary heart disease (CHD, n=70), SCD (n=53) and control (n=75) group. Significant associations were identified for 10, 4 and 6 SNPs in CHD, SCD and sudden death from CHD, respectively, using the χ2 test. The SNPs obtained by binary logistic regression may be used to assess and predict the risk of disease. The predictive accuracy of the SNPs in each prediction model and their area under the receiver operating characteristic curve (AUC) values were determined. The AUC of the four SNPs (rs12429889, rs10829156, rs16942421 and rs12155623) to predict CHD was 0.928, the AUC of the six SNPs (rs2389202, rs2982694, rs10183640, rs597503, rs16942421 and rs12155623) to predict SCD was 0.922 and the AUC of the four SNPs (rs16866933, rs4621553, rs10829156 and rs12155623) to predict sudden death from CHD was 0.912. The multifactor dimensionality reduction values were as follows: 0.8690 (prediction model of CHD), 0.7601 (prediction model of SCD) and 0.7628 (prediction model of sudden death from CHD). Taken together, the results of the present study suggested that these SNPs have considerable potential for application in genetic tests to predict CHD or SCD. However, further studies are required to investigate the putative functions of these SNPs. [ABSTRACT FROM AUTHOR]

[Display omitted] • Cd inhibited the viability of MDA-MB-231 cells in a time- and dose-dependent manner. • Caspase-inhibitor of z-VAD reduced Cd-induced LDH release and pyroptosis. • NAC attenuated Cd-activated ROS, NLRP3 and inflammation-related genes. • MKK3/6/p38 MAPK/NF-κB pathway was activated. • Cd induced GSDME-dependent pyroptosis by activation of bax/caspase-3/GSDME pathway. Cadmium (Cd) exposure can exert an impact on carcinogenicity of breast cancer, however, the mechanism is not fully understood in triple-negative breast cancer (TNBC). We performed a TNBC MDA-MB-231 cell model and assessed the toxic effect of Cd exposure (0, 10, 20, 50, 60, 80 μM). Cd reduced cell viability in a time- and dose-dependent manner, followed by cell cycle arrest in S phase with alterations of cyclin 1A1, cyclin 1D1 and CDK2. Lactate dehydrogenase (LDH) release, apoptosis and pyroptosis were increased, which were relieved by z-VAD. Elevated ROS and NLRP3, caspase-1, IL-1β and IL-18 were detected, which was attenuated by N-acetylcysteine. Increased bax and decreased caspase-8, caspase-9 and caspase-3 were found. gasdermin E (GSDME) was activated with cleavage of GSDME-NT, which was retarded by z-VAD. Additionally, p38 MAPK signaling pathway was activated. Our data demonstrate GSDME-activated pyroptosis in Cd toxicity, implying a potential impact on TNBC. [ABSTRACT FROM AUTHOR]

Abstract: We have studied the chromosomes of lymphocytes of the residents in a high background radiation area (HBRA) and a control area (CA) in the southern China. The level of natural radiation in HBRA is three to five times higher than that in control area. Unstable type aberrations (dicentrics and rings) were analyzed in 22 individuals in HBRA and 17 individuals in CA. Stable type aberrations (translocations) were examined in 28 elderly individuals and 6 children in HBRA and 24 elderly individuals and 8 children in CA. There was statistically significant difference in the increasing rates between those two areas in adult. In case of stable aberrations, the frequencies were much higher than those of the unstable aberrations both in HBRA and in control. Statistically, there was no difference in the frequencies between HBRA and CA, but the frequencies in children were lower than those in elderly individuals in both groups. Individual variation was small in children while that in adults was large. The amount of chromosome aberrations induced by radiation is not significant when it is compared with that induced by other mutagenic factors such as smoking in the Southern China. [Copyright &y& Elsevier]

It is widely understood that most system downtime is accounted for by programming errors and administration time. However, a growing body of work has indicted an increasing cause of downtime may stem from transient errors in computer system hardware due to external factors, such as cosmic rays. This work indicates that moving to denser semiconductor technologies at lower voltages has the potential to increase these transient errors. In this paper, we investigate the susceptibility of commodity operating systems and applications on commodity PC processors to these soft-errors and we introduce ideas regarding the improved recovery from these transient errors in software. Our results indicate that, for the Linux kernel and a Java virtual machine running sample workloads, many errors are not activated, mostly due to overwriting. In addition, given current and upcoming microprocessor support, our results indicate that those errors activated, which would normally lead to system reboot, need not be fatal to the system if software knowledge is used for simple software recovery. Together, they indicate the benefits of simple memory soft error recovery handling in commodity processors and software. [ABSTRACT FROM AUTHOR]

The Soil Moisture Experiment in the Luan River (SMELR) was conducted from 2017 to 2018 in the semi-arid Luan River watershed located in the North of China. One of the objectives of SMELR is to serve as an assessment tool and demonstration for a new Terrestrial Water Resources Satellite (TWRS) concept with one-dimensional synthetic aperture microwave techniques, for which soil moisture retrieval under variable satellite observing configurations (mainly in terms of incidence angels) is the greatest challenge. This proposed mission is targeted to provide continuity for the current satellite L-band microwave observations, and further improve the accuracy and spatial resolution of soil moisture mapping through the synergistic use of active, passive and optical remote sensing data. Multi-resolution, multi-angle and multi-spectral airborne data were obtained four times over a 70 km by 12 km area in the Shandian River basin, and one time over a 165 km by 5 km area that includes the Xiaoluan River basin. The near surface soil moisture (0 cm–5 cm) was measured extensively on the ground in fifty 1 km by 2 km quadrats (targeted to compare with the airborne radiometer), and two hundred and fifty 200 m by 200 m quadrats corresponding to radar observations. Two networks were established for continuous measurement of the soil moisture and temperature profile (3 cm, 5 cm, 10 cm, 20 cm, 50 cm) and precipitation in the Shandian and Xiaoluan River basin, respectively. Supporting ground measurements also included ground temperature, vegetation water content, surface roughness, continuous measurement of microwave emission and backscatter at a pasture site, reflectance of various land cover types, evapotranspiration and aerosol observations. Preliminary results within the experimental area indicate that (1) the near surface soil moisture spatial variability at a 200 m scale was up to ~0.1 cm3/cm3 at an intermediate value of ~0.35 cm3/cm3. (2) The difference of soil and vegetation temperature in grass and croplands reach its maximum of 11 K around solar noon time, and the soil temperature gradient is largest at around 15 P.M. (3) Both the airborne and ground measurements cover a wide range of conditions. The L-band active and passive observations exhibit a large variation of ~30 dB and ~80 K, respectively, corresponding to soil moisture range from 0.1 cm3/cm3 to 0.5 cm3/cm3. The sensitivity of both active and passive data to soil moisture is compared at corresponding spatial resolutions and show high information complementarity for better accuracy and resolution soil moisture retrieval. • First airborne optical and active-passive microwave experiment conducted in China • Demonstration of the active-passive microwave synergy at varying incidence angles • Addressing prelaunch science questions of the terrestrial water resources mission [ABSTRACT FROM AUTHOR]