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197 results on '"Chen, Fang-Hsin"'

2. Process Verification of Magnetic Ion Embedded Nanodiamonds Using Secondary Ion Mass Spectroscopy

Author

Lin, Bo-Rong, Chen, Chien-Hsu, Kunuku, Srinivasu, Hsiao, Tung-Yuan, Yu, Hung-Kai, Chen, Tzung-Yuang, Chang, Yu-Jen, Liao, Li-Chuan, Chang, Chun-Hsiang, Chen, Fang-Hsin, Niu, Huan, and Lee, Chien-Ping

Subjects
Physics - Applied Physics
Abstract

Ion implantation is used to create magnetic ion embedded nanodiamonds for use in a wide range of biological and medical applications; however, the effectiveness of this process depends heavily on separating magnetic nanodiamonds from non-magnetic ones. In this study, we use secondary ion mass spectrometry to assess the distribution of magnetic ions and verify the success of separation. When applied to a series of iron/manganese embedded nanodiamonds, the sorting tool used in this study proved highly effective in selecting magnetic nanodiamonds. This paper also discusses the major challenges involved in the further development of this technology., Comment: 12 pages, 3 figures

Published
2019

3. Iron Embedded Magnetic Nanodiamonds for in vivo MRI Contrast Enhancement

Author

Lin, Bo-Rong, Chen, Chien-Hsu, Chang, Chun-Hsiang, Kunuku, Srinivasu, Chen, Tzung-Yuang, Hsiao, Tung-Yuan, Yu, Hung-Kai, Chang, Yu-Jen, Liao, Li-Chuan, Chen, Fang-Hsin, Niu, Huan, and Lee, Chien-Ping

Subjects
Physics - Medical Physics, Physics - Biological Physics
Abstract

Although nanodiamonds have long being considered as a potential tool for biomedical research, the practical in vivo application of nanodiamonds remains relatively unexplored. In this paper, we present the first application of in vivo MRI contrast enhancement using only iron embedded magnetic nanodiamonds. MR image enhancement was clearly demonstrated in the rendering of T2-weighted images of mice obtained using an unmodified commercial MRI scanner. The excellent contrast obtained using these nanodiamonds opens the door to the non-invasive in vivo tracking of NDs and image enhancement. In the future, one can apply these magnetic nanodiamonds together with surface modifications to facilitate drug delivery, targeted therapy, localized thermal treatment, and diagnostic imaging., Comment: 19 pages, 6 figures

Published
2019
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6. Circulating M-MDSC Levels as an Assessment Marker for Post-Treatment Tumor Progression in Recurrent HNC Patients Following Radiation Therapy: A Case Series.

Author

Chang, Chun-Hsiang, Chen, Fang-Hsin, Wang, Ling-Wei, and Chiang, Chi-Shiun

Subjects
*BORON-neutron capture therapy, *MYELOID-derived suppressor cells, *CYTOTOXIC T cells, *IMAGE-guided radiation therapy, *INTENSITY modulated radiotherapy, *HEAD & neck cancer
Abstract

Background: In advanced head and neck cancer (HNC) patients, 50–60% experience loco-regional relapse and distant metastasis. Boron neutron capture therapy (BNCT) has shown remarkable therapeutic response in recurrent HNC, but there is still a 70% chance of local recurrence. This study aimed to identify a suitable liquid biomarker to assess patient response following BNCT. Myeloid-derived suppressor cells (MDSCs) are immune-suppressive cells that inhibit cytotoxic T cells. Circulating MDSC levels have been linked to the clinical stage and prognosis in HNSCC. Methods: Five patients with recurrent head and neck cancer underwent a treatment regimen that commenced with BNCT, followed by fractionated image-guided intensity-modulated radiotherapy (IG-IMRT). Liquid biopsy analysis via flow cytometry and tumor volume analysis by clinical imaging were conducted at three stages: before BNCT, before the first fraction of IG-IMRT, and one month after the last fraction of IG-IMRT. Results: Compared to other MDSC subtypes, monocytic MDSCs (M-MDSCs) exhibited a notable correlation with tumor volume. This strong correlation was observed at all testing time points except one month after BNCT treatment. Conclusions: This case series highlights a strong link between tumor size and circulating M-MDSC levels before BNCT and one month after the last IG-IMRT treatment in recurrent head and neck cancer patients. These results suggest that the level of circulating M-MDSCs could be a marker for monitoring tumor progression in recurrent HNC patients following radiation therapy, including BNCT. [ABSTRACT FROM AUTHOR]

Published
2024
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24. Proton FLASH Irradiation Platform for Small Animal Setup at Chang Gung Memorial Hospital

Author

Hsiao, Tung-Yuan, Wang, Lu-Kai, Chen, Tzung-Yuang, Yu, Ching-Fang, Pan, Cheng-Ya, Wang, Chun-Chieh, Lin, Chien-Yu, Cho, I-Chun, Chen, Fang-Hsin, Niu, Huan, and Chen, Chien-Hsu

Abstract

The purpose of this study was to create a platform for conducting small animal proton FLASH irradiation at a research-specific therapy center using an auxiliary proton beam line. A dose monitoring system was implemented to provide real-time irradiation dose and dose rate time structure information, utilizing a transmission ionization chamber (TIC), a plastic scintillator-based beam position monitor, and a poor man faraday cup (PMFC). Both the TIC and PMFC were equipped with a custom-made fast-reading current integral electronics device, allowing for real-time dose monitoring with a resolution of 0.5 ms. Experimental testing showed that the maximum dose that could be delivered to the target in a single pulse of 230-MeV protons was approximately 20 Gy, during a pulse period of 199 ms, at a dose rate of 100 Gy/s. The establishment of a dedicated proton research platform at Chang Gung Memorial Hospital has enabled investigation of the biological effects of FLASH irradiation on small animals. The final setup data serves as a useful reference for both planning future experiments and improving the facility for beam users. This study’s achievement provides a valuable foundation for further research on proton FLASH irradiation, potentially offering new insights into its applications for cancer treatment.

Published
2024
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30. Additional file 1 of Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy

Author

Tsai, Shiao-Wen, Lo, Chang-Yun, Yu, Shang-Yang, Chen, Fang-Hsin, Huang, Hsiao-Chieh, Wang, Lu-Kai, and Liaw, Jiunn-Woei

Abstract

Additional file 1. Table S1: Parameters setup of the wavelengths of the excitation (Ex) lasers and the passbands of the emission (Em) filters of LSCM for inducing and detecting the fluorescence of different biomarkers (kits) for ROS and organelles in cells. Fig. S1: Cell fluorescence images of LSCM for labeled ROS irradiated by Cs-137 with a dose of 6 Gy (magnification: ×20); Fig. S2: Cell fluorescence images of LSCM for labeled activate mitochondria, 48 hours after irradiation of 6 Gy Cs-137 (magnification: ×20); Fig. S3: Cell fluorescence images of LSCM for labeled cytoskeletons, 48 hours after the irradiation of Cs-137 with a dose of 6 Gy (magnification: ×100).

Published
2023
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31. Secondary ion mass spectrometry to verify the implantation of magnetic ions in nanodiamonds.

Author

Lin, Bo-Rong, Wang, Chiung-Chi, Chen, Chien-Hsu, Kunuku, Srinivasu, Hsiao, Tung-Yuan, Yu, Hung-Kai, Chen, Tzung-Yuang, Chang, Yu-Jen, Liao, Li-Chuan, Chang, Chun-Hsiang, Chen, Fang-Hsin, Niu, Huan, and Lee, Chien-Ping

Subjects
SECONDARY ion mass spectrometry, NANODIAMONDS, MAGNETIC ions, ION implantation, SILICON wafers, IRON ions
Abstract

Ion implantation is used to create nanodiamonds (NDs) with embedded magnetic ions for use in a wide range of biological and medical applications; however, the effectiveness of this process depends heavily on separating magnetic NDs from nonmagnetic ones. In this study, we use secondary ion mass spectrometry to verify the implantation of magnetic ions in NDs and the success of separation. When applied to a series of NDs with embedded iron or manganese ions, the sorting tool used in this study proved highly effective in selecting magnetic NDs. Besides, multienergy ion implantation and precise thickness control of NDs coating on the silicon wafer were suggested to improve this technology. [ABSTRACT FROM AUTHOR]

Published
2019
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36. Glycolytic Plasticity of Metastatic Lung Cancer Captured by Noninvasive 18 F-FDG PET/CT and Serum 1 H-NMR Analysis: An Orthotopic Murine Model Study.

Author

Chung, Yi-Hsiu, Hung, Tsai-Hsien, Yu, Ching-Fang, Tsai, Cheng-Kun, Weng, Chi-Chang, Jhang, Fujie, Chen, Fang-Hsin, and Lin, Gigin

Subjects
GLYCOLYSIS, LUNG cancer, METASTASIS, PHENOTYPIC plasticity, RECEIVER operating characteristic curves, LUNG tumors
Abstract

We aim to establish a noninvasive diagnostic platform to capture early phenotypic transformation for metastasis using 18F-FDG PET and 1H-NMR-based serum metabolomics. Mice with implantation of NCI-H460 cells grew only primary lung tumors in the localized group and had both primary and metastatic lung tumors in the metastatic group. The serum metabolites were analyzed using 1H-NMR at the time of PET/CT scan. The glycolysis status and cell proliferation were validated by Western blotting and staining. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic accuracy of SUVmean and serum metabolites in metastasis. In the metastatic mice, the SUVmean of metastatic tumors was significantly higher than that of primary lung tumors in PET images, which was supported by elevated glycolytic protein expression of HK2 and PKM2. The serum pyruvate level in the metastatic group was significantly lower than that in the localized group, corresponding to increased pyruvate-catalyzed enzyme and proliferation rates in metastatic tumors. In diagnosing localized or metastatic tumors, the areas under the ROC curves of SUVmean and pyruvate were 0.92 and 0.91, respectively, with p < 0.05. In conclusion, the combination of 18F-FDG PET and 1H-NMR-based serum metabolomics demonstrated the feasibility of a glycolytic platform for diagnosing metastatic lung cancers. [ABSTRACT FROM AUTHOR]

Published
2023
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39. Systematic identification of clinically relevant miRNAs for potential miRNA-based therapy in lung adenocarcinoma

Author

Liu, Shu-Hsuan, primary, Hsu, Kai-Wen, additional, Lai, Yo-Liang, additional, Lin, Yu-Feng, additional, Chen, Fang-Hsin, additional, Peng, Pei-Hwa, additional, Lin, Li-Jie, additional, Wu, Heng-Hsiung, additional, Li, Chia-Yang, additional, Wang, Shu-Chi, additional, Wu, Min-Zu, additional, Sher, Yuh-Pyng, additional, and Cheng, Wei-Chung, additional

Published
2021
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42. LipidSig: a web-based tool for lipidomic data analysis

Author

Lin, Wen-Jen, primary, Shen, Pei-Chun, additional, Liu, Hsiu-Cheng, additional, Cho, Yi-Chun, additional, Hsu, Min-Kung, additional, Lin, I-Chen, additional, Chen, Fang-Hsin, additional, Yang, Juan-Cheng, additional, Ma, Wen-Lung, additional, and Cheng, Wei-Chung, additional

Published
2021
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48. Synthetic 4-Hydroxy Auxarconjugatin B, a Novel Autophagy Inducer, Attenuates Gouty Inflammation by Inhibiting the NLRP3 Inflammasome

Author

Hsieh, Chih-Yu, primary, Li, Lan-Hui, additional, Lam, Yulin, additional, Fang, Zhanxiong, additional, Gan, Chin Heng, additional, Rao, Yerra Koteswara, additional, Chiu, Hsiao-Wen, additional, Wong, Wei-Ting, additional, Ju, Tz-Chuen, additional, Chen, Fang-Hsin, additional, Chernikov, Oleg V., additional, Liu, May-Lan, additional, Hsu, Chung-Hua, additional, and Hua, Kuo-Feng, additional

Published
2020
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