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1. Nonlinear Realization of a Dynamical Poincaré Symmetry by a Field-Dependent Diffeomorphism

Author: Bazeia, D. and Jackiw, R.
Published: 1998
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2. Comparative Genomics Reveals Novel Target Genes Towards Specific Control of Plant-Parasitic Nematodes

Author: Priscila Grynberg, Corinne Rancurel, José Dijair Antonino, Robert Neil Gerard Miller, Etienne Danchin, Marcos Mota do Carmo Costa, Roberto C. Togawa, Leticia Dias de Freitas, Patricia Messenberg Guimaraes, Ana Cristina Miranda Brasileiro, Maria Fatima Grossi-de-Sa, Embrapa Genetic Resources and Biotechnology, University of Bras ılia, Brazilian Agricultural Research Corporation (Embrapa), Universidade de Brasilia [Brasília] (UnB), Universidade Federal Rural de Pernambuco (UFRPE), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Universidade Católica de Brasília (UCB), University of Brasilia [Brazil] (UnB), CNPq PVE Science without Borders Program 401541/2014-2, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) 88887.310583/2018-00, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) 465480/2014-4, INCT PlantStress Biotech (FAPDF project) 193.001.265/2017, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) 001, and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)
Subjects: 0106 biological sciences, 0301 basic medicine, lcsh:QH426-470, Gene Transfer, Horizontal, Nematoda, de novo gene birth, [SDV]Life Sciences [q-bio], Genomics, comparative genomics, Computational biology, 01 natural sciences, Genome, Article, Host-Parasite Interactions, 03 medical and health sciences, Phylogenomics, Genetics, Meloidogyne incognita, Animals, anatomy_morphology, Computer Simulation, Tylenchoidea, Gene, Phylogeny, Genetics (clinical), Plant Diseases, 2. Zero hunger, Comparative genomics, Genome, Helminth, biology, Effector, plant-parasitic nematodes, phylogenomics, Helminth Proteins, Plants, biology.organism_classification, horizontal gene transfers, parasite-specific genes, lcsh:Genetics, Gene Ontology, 030104 developmental biology, Nematode, Gene Expression Regulation, pest control, 010606 plant biology & botany
Abstract: Plant-parasitic nematodes cause expressive annual yield losses to worldwide agricultural production. Most cultivated plants have no known resistance against nematodes and the few bearing a resistance gene can be overcome by certain species. The chemical methods that have been deployed to control nematodes were largely banned from use due to their poor specificity and high toxicity. Hence, there is an urgent need for the development of cleaner and more specific control methods. Recent advances in nematode genomics, including in phytoparasitic species, provide an unprecedented opportunity to identify genes and functions specific to these pests. Using phylogenomics, we compared 61 nematode genomes, including 16 for plant-parasitic species and identified more than 24,000 protein families specific to these parasites. In the genome of Meloidogyne incognita, one of the most devastating plant parasites, we found ca. 10,000 proteins with orthologs restricted only to phytoparasitic species and no further homology in protein databases. Among these phytoparasites-specific proteins, ca. 1,000 shared the same properties as known secreted effectors involved in essential parasitic functions. Of those, 68 were novel and showed strong expression during the endophytic phase of the nematode life cycle, based on both RNA-seq and RT-qPCR analyses. Besides effector candidates, transcription-related and neuro-perception functions were enriched in phytoparasites-specific proteins, revealing interesting targets for nematode control methods. This phylogenomics analysis, constitutes an unprecedented resource for the further understanding of the genetic basis of nematode adaptation to phytoparasitism and for the development of more efficient control methods.
Published: 2020
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3. Rational Design of W-Doped Ag3PO4 as an Efficient Antibacterial Agent and Photocatalyst for Organic Pollutant Degradation

Author: Vinícius Teodoro, Natalia Jacomaci, Amanda F. Gouveia, Mayara Mondego Teixeira, Isaac Sánchez-Montes, Elson Longo, André Perrin, Camila Cristina de Foggi, Letícia Guerreiro da Trindade, Christiane Perrin, Juan Andrés, Aline B. Trench, José M. Aquino, Thales R. Machado, Federal University of São Carlos (UFSCar), Universidade Estadual de Campinas = University of Campinas (UNICAMP), Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Université de Rennes (UR), Universitat Jaume I, Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/07296-2, 2017/12594-3, 2019/01732-1, 2019/13507-2], Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior. Brasil (CAPES) Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001], Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [142035/2017-3], Universitat Jaume I [UJI-B2016-25, UJI-B2019-30], Ministerio de Ciencia, Innovacion y Universidades (Spain) [PGC2018-094417-B-I00], Universidade Estadual de Campinas (UNICAMP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Universidade Federal de São Carlos (UFSCar), Universidade Estadual Paulista (Unesp), Univ Rennes 1, and Univ Jaume 1
Subjects: General Chemical Engineering, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, Human health, Degradation, Doping, [CHIM]Chemical Sciences, Photocatalysis, QD1-999, Materials, Antibacterial agent, Pollutant, Waste management, Rational design, General Chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Chemistry, 13. Climate action, Environmental science, Degradation (geology), Irradiation, 0210 nano-technology
Abstract: Made available in DSpace on 2021-06-25T12:20:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-22 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Universitat Jaume I Ministerio de Ciencia, Innovacion y Universidades (Spain) Bacterial and organic pollutants are major problems with potential adverse impacts on human health and the environment. A promising strategy to alleviate these impacts consists in designing innovative photocatalysts with a wider spectrum of application. In this paper, we report the improved photocatalytic and antibacterial activities of chemically precipitated Ag3PO4 microcrystals by the incorporation of W at doping levels 0.5, 1, and 2 mol %. The presence of W directly influences the crystallization of Ag3PO4, affecting the morphology, particle size, and surface area of the microcrystals. Also, the characterization via experimental and theoretical approaches evidenced a high density of disordered [AgO4], [PO4], and [WO4] structural clusters due to the substitution of P5+ by W6+ into the Ag3PO4 lattice. This leads to new defect-related energy states, which decreases the band gap energy of the materials (from 2.27 to 2.04 eV) and delays the recombination of e'-h(center dot) pairs, leading to an enhanced degradation process. As a result of such behaviors, W-doped Ag3PO4 (Ag3PO4:W) is a better visible-light photocatalyst than Ag3PO4, demonstrated here by the photodegradation of potential environmental pollutants. The degradation of rhodamine B dye was 100% in 4 min for Ag3PO4:W 1%, and for Ag3PO4, the obtained result was 90% of degradation in 15 min of reaction. Ag3PO4:W 1% allowed the total degradation of cephalexin antibiotic in only 4 min, whereas pure Ag3PO4 took 20 min to achieve the same result. For the degradation of imidacloprid insecticide, Ag3PO4:W 1% allowed 90% of degradation, whereas Ag3PO4 allowed 40%, both in 20 min of reaction. Moreover, the presence of W-dopant results in a 16-fold improvement of bactericidal performance against methicillin-resistant Staphylococcus aureus. The outstanding results using the Ag3PO4:W material demonstrated its potential multifunctionality for the control of organic pollutants and bacteria in environmental applications. Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP, Brazil Univ Fed Sao Carlos, Dept Chem, CDMF, BR-13565905 Sao Carlos, SP, Brazil Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP, Brazil Sao Paulo State Univ, Dept Chem, BR-17033360 Bauru, SP, Brazil Sao Paulo State Univ, Chem Inst, BR-14800060 Araraquara, SP, Brazil Univ Rennes 1, F-35042 Rennes, France Univ Jaume 1, Dept Analyt & Phys Chem, Castellon de La Plana 12071, Spain Sao Paulo State Univ, Dept Chem, BR-17033360 Bauru, SP, Brazil Sao Paulo State Univ, Chem Inst, BR-14800060 Araraquara, SP, Brazil FAPESP: 2013/07296-2 FAPESP: 2017/12594-3 FAPESP: 2019/01732-1 FAPESP: 2019/13507-2 CAPES: 001 CNPq: 142035/2017-3 Universitat Jaume I: UJI-B2016-25 Universitat Jaume I: UJI-B2019-30 Ministerio de Ciencia, Innovacion y Universidades (Spain): PGC2018-094417-B-I00
Published: 2020
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4. Design of polyurea networks containing anticancer and anti‐inflammatory drugs for dual drug delivery purposes

Author: Mariane A. de Resende, Lucia H. G. M. C. Macêdo, Ricardo de Oliveira, Yann Molard, Eduardo F. Molina, Gabriele A. Pedroza, Maria Amela-Cortes, Universidade de Franca, Synthèse Caractérisation Analyse de la Matière (ScanMAT), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001], Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [306271/2017-6], FAPESP SPRINT Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2017/50286-9], CNRS Centre National de la Recherche Scientifique (CNRS) European Commission, Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2019/17860-9, 2020/06531-1], Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université de Rennes (UR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: spectroscopy, Polymers and Plastics, medicine.drug_class, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Anti-inflammatory, drug delivery systems, polyetheramine, chemistry.chemical_compound, Materials Chemistry, medicine, [CHIM]Chemical Sciences, glass transition, Polyurea, elastomers, General Chemistry, DUAL (cognitive architecture), 021001 nanoscience & nanotechnology, Combinatorial chemistry, 0104 chemical sciences, Surfaces, Coatings and Films, [CHIM.POLY]Chemical Sciences/Polymers, chemistry, Drug delivery, 0210 nano-technology
Abstract: International audience; Naproxen (Nap), a non-steroidal anti-inflammatory drug, and 5-fluorouracil (5FU), an anticancer drug, were easily incorporated into a polyurea xerogel. Polyureas (PUr) were synthesized by one pot reaction via sol-gel chemistry. Soft segments were based on polyetheramine-PEO and hard segments were based on a hexamethylene diisocyanate trimer (HDI). The final materials were characterized by Fourier transform infrared (FTIR) spectroscopy, small-angle X-ray scattering (SAXS), and differential scanning calorimetry (DSC). Polyurea can assume many forms revealing its versatility in terms of produced shapes. In good agreement with FTIR, SAXS studies showed a microphase separated structure arising from inter-hard-domain spacings, which remains unchanged after incorporation of the drugs. DSC analysis demonstrated that the PEO chain mobility was poorly affected after the drugs incorporation due to the solvation of the drugs through the network. The water uptake and the amount of drug released agree well, playing an important role for controlled Nap and 5FU release. This work opens positive perspectives for ocular drug delivery and wound management, due to the easy processability of the xerogel, which has high feasibility to be moldable as pharmaceutical devices for contact lenses and transdermal patches purposes containing distinct therapeutic agents.
Published: 2021
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5. Pareto solutions as limits of collective traps: an inexact multiobjective proximal point algorithm

Author: G. C. Bento, J. X. Cruz Neto, L. V. Meireles, A. Soubeyran, Universidade Federal de Goiás [Goiânia] (UFG), Universidade Federal do Piauí (UFPI), Instituto Federal Goiano, Aix-Marseille Sciences Economiques (AMSE), École des hautes études en sciences sociales (EHESS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Fundacao de Amparo a Pesquisa do Estado do Goias (FAPEG) - Grant: 201710267000532, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)Grant: 308330/2018-8& 314106/2020-0, and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Subjects: TheoryofComputation_MISCELLANEOUS, Variational rationality, Worthwhile moveTrap, Riemannian manifold, General Decision Sciences, Multiobjective proximal method, Approximate solution, [MATH]Mathematics [math], Management Science and Operations Research, [SHS.ECO]Humanities and Social Sciences/Economics and Finance
Abstract: International audience; In this paper we introduce a definition of approximate Pareto efficient solution as well as a necessary condition for such solutions in the multiobjective setting on Riemannian manifolds. We also propose an inexact proximal point method for nonsmooth multiobjective optimization in the Riemannian context by using the notion of approximate solution. The main convergence result ensures that each cluster point (if any) of any sequence generated by the method is a Pareto critical point. Furthermore, when the problem is convex on a Hadamard manifold, full convergence of the method for a weak Pareto efficient solution is obtained. As an application, we show how a Pareto critical point can be reached as a limit of traps in the context of the variational rationality approach of stay and change human dynamics.
Published: 2022
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6. A new species of Pleuropholis (Teleostei: Pleuropholidae) from the Upper Cretaceous Akrabou Formation of southeastern Morocco, with comments on the evolutionary history of the genus

Author: Paulo M. Brito, Romain Vullo, Universidade do Estado do Rio de Janeiro [Rio de Janeiro] (UERJ), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Géosciences Rennes (GR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) 305118/2021-8, and Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio De Janeiro (FAPERJ)E-26/201.172/2022
Subjects: Actinopterygii, New taxon, Africa, Neopterygii, Pleuropholid fish, Cretaceous Research, Paleontology, Cenomanian, [SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/Paleontology
Abstract: International audience; A pleuropholid fish is described for the first time from the Upper Cretaceous (Cenomanian) Akrabou Formation, Agoult locality, southeastern Morocco. The new material, consisting of a single complete specimen, is attributed to a new species, Pleuropholis danielae, and is diagnosed by its slender body shape, the absence of a leptolepid notch in the dentary, a broad maxilla, covering almost the entirely lower jaw, and flank scales with an unserrated posterior margin. This species represents the last known occurrence of Pleuropholidae, a group so far unknown from the Late Cretaceous.
Published: 2023
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7. 3D model related to the publication: An eosimiid primate of SouthAsian affinities in the Paleogene of Western Amazonia and the originof New World monkeys

Author: Marivaux, Laurent, Negri, Francisco, Ribeiro, Ana Maria, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Universidade Federal do Acre, Cruzeiro Do Sul (UFAC), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq 310023/2021-1 and 310948/2021-5, CNPq/MCTI/CONFAP-FAPS PROTAX 22/2020 441626/2020-3, and Fapergs 21/2551-0000781-8)., São Paulo Research Foundation (FAPESP, process n°2011/14080-0)., LabEx CEBA (ANR-10-LABX-25-01, and strategic project EMERGENCE.
Subjects: early Anthropoidea, Eosimiidae, Paleobiogeography, [SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/Paleontology, Brazilian Amazonia, Platyrrhini
Abstract: International audience; This contribution contains the three-dimensional digital model of one isolated fossil tooth of an anthropoid primate (Ashaninkacebus simpsoni), discovered in sedimentary deposits located on the upper Rio Juru´a in State of Acre, Brazil (Western Amazonia). This fossil was described, figured and discussed in the following publication: Marivaux et al. (2023), An eosimiid primate of South Asian affinities in the Paleogene of Western Amazonia and the origin of New World monkeys. Proceedings of the National Academy of Sciences USA. https://doi.org/10.1073/pnas.2301338120
Published: 2023
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8. COVID-19-Associated Pulmonary Aspergillosis Isolates Are Genomically Diverse but Similar to Each Other in Their Responses to Infection-Relevant Stresses

Author: Mead, Matthew E., de Castro, Patricia Alves L., Steenwyk, Jacob L., Gangneux, Jean-Pierre, Hoenigl, Martin, Prattes, Juergen, Rautemaa‐richardson, Riina, Guegan, H, Moore, C, Lass-Floerl, Cornelia, Reizine, Florian, Valero, Clara, van Rhijn, Norman J., Bromley, Michael J, Rokas, Antonis H., Goldman, Gustavo H., Gago, Sara, Vanderbilt University [Nashville], Universidade de São Paulo = University of São Paulo (USP), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), Hôpital Sud [CHU Rennes], CHU Pontchaillou [Rennes], Medical University Graz, University of Manchester [Manchester], Leopold Franzens Universität Innsbruck - University of Innsbruck, Howard Hughes Medical Institute through the James H. Gilliam Fellowships for Advanced Study program, National Science Foundation [DEB-2110404], National Institutes of Health/National Institute of Allergy and Infectious Diseases [R56 AI146096, R01 AI153356], Burroughs Wellcome Fund, NIHR Manchester Biomedical Research Centre, Fungal Infection Trust, Manchester Academy of Health Sciences, Dowager Countess Eleanor Peel Trust, Wellcome Trust [219551/Z/19/], Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) from Brazil [2021/04977-5, BEPE 2020/01131-5], Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) from Brazil [301058/2019-9, 404735/2018-5, 163550/2020-4], and National Institutes of Health/National Institute of Allergy and Infectious Diseases grant [R01AI153356]
Subjects: strain heterogeneity, SARS-CoV-2, Aspergillus fumigatus, [SDV]Life Sciences [q-bio], fungal pathogen, coinfection
Abstract: International audience; Secondary infections caused by the pulmonary fungal pathogen Aspergillus fumigatus are a significant cause of mortality in patients with severe coronavirus disease 19 (COVID-19). Even though epithelial cell damage and aberrant cytokine responses have been linked to susceptibility to COVID-19-associated pulmonary aspergillosis (CAPA), little is known about the mechanisms underpinning copathogenicity. Here, we analyzed the genomes of 11 A. fumigatus isolates from patients with CAPA in three centers from different European countries. CAPA isolates did not cluster based on geographic origin in a genome-scale phylogeny of representative A. fumigatus isolates. Phenotypically, CAPA isolates were more similar to the A. fumigatus A1160 reference strain than to the Af293 strain when grown in infection-relevant stresses, except for interactions with human immune cells wherein macrophage responses were similar to those induced by the Af293 reference strain. Collectively, our data indicate that CAPA isolates are genomically diverse but are more similar to each other in their responses to infection-relevant stresses. A larger number of isolates from CAPA patients should be studied to better understand the molecular epidemiology of CAPA and to identify genetic drivers of copathogenicity and antifungal resistance in patients with COVID-19. IMPORTANCE Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) has been globally reported as a life-threatening complication in some patients with severe COVID-19. Most of these infections are caused by the environmental mold Aspergillus fumigatus, which ranks third in the fungal pathogen priority list of the WHO. However, little is known about the molecular epidemiology of Aspergillus fumigatus CAPA strains. Here, we analyzed the genomes of 11 A. fumigatus isolates from patients with CAPA in three centers from different European countries, and carried out phenotypic analyses with a view to understanding the pathophysiology of the disease. Our data indicate that A. fumigatus CAPA isolates are genomically diverse but are more similar to each other in their responses to infection-relevant stresses.
Published: 2023
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9. The tribes Kampimodromini Kolodochka and Typhlodromipsini Chant and McMurtry (Mesostigmata: Phytoseiidae) in the Serra do Espinhaço, Brazil, with a key to the Brazilian species of Typhlodromips De Leon

Author: Francisco Ferragut, Denise Navia, Universitat Politècnica de València (UPV), Instituto Agroforestal Mediterraneo (IAM), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil, for granting the fellowship to the second author (DN) (PP, Process N degrees 3121322017-4).
Subjects: taxonomy, Proprioseius, Insect Science, [SDV]Life Sciences [q-bio], neotropical, predatory mites, Neoparaphytoseius, Typhlodromips
Abstract: International audience; Phytoseiid mites of the tribes Kampimodromini and Typhlodromipsini collected from the Serra do Espinhaço (Espinhaço range) are reported here. Two species are proposed as new, Neoparaphytoseius moraesi sp. nov. and Typhlodromips ovametapodalis sp. nov. Typhlodromips constrictatus (El-Banhawy) is redescribed in detail including the first description of the male and the female insemination apparatus. The redescription of the female and the first description of the male and deutonymph of Proprioseius gibbus Moraes and Denmark are given. Complementary descriptions of the females of T. ariri Gondim Jr. and Moraes and T. japi Lofego, Demite and Feres are also included. A key is provided for the separation of the Brazilian species of the genus Typhlodromips.
Published: 2022
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10. Parasitoids (Hymenoptera) of Mealybug Pests (Hemiptera: Pseudococcidae) from Southern Brazil: Molecular and Morphological Characterization

Author: Daniel Alejandro Aquino, Thibaut Malausa, Didier Crochard, Vitor Cezar Pacheco da Silva, Ferran Palero, Marcos Botton, VITOR CEZAR PACHECO DA SILVA, Unidad de Entomología, Facultad de Agronomía, Univ de la República, Montevideo, Uruguay, FERRAN PALERO, Cavanilles Institute of Biodiversity and Evolutionary Biology, Univ of Valencia, Paterna, Spain., DANIEL ALEJANDRO AQUINO, Centro de Estudios Parasitológicos y de Vectores (CONICET – UNLP), La Plata, Buenos Aires, Argentina, DIDIER CROCHARD, Institut Sophia Agrobiotech INRA, Sophia Antipolis, PACA, France, THIBAUT MALAUSA, Institut Sophia Agrobiotech INRA, Sophia Antipolis, PACA, France, MARCOS BOTTON, CNPUV, Universidad de la República [Montevideo] (UDELAR), Universidad Nacional de Mar del Plata [Mar del Plata] (UNMdP), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Brazilian Agricultural Research Corporation (Embrapa), Universitat de València (UV), London Nat. Hist. Museum, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)BPI from Institut Sophia Agrobiotech - INRA, and European Project: 324475,EC:FP7:PEOPLE,FP7-PEOPLE-2012-IAPP,COLBICS(2013)
Subjects: Pseudococcidae, biology, Biología, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, biology.organism_classification, Hymenoptera, Pseudococcus viburni, Biological control agents, Parasitoid, Hemiptera, Signiphoridae, Pseudococcus, Anagyrus, Encyrtidae, Fruit, Insect Science, Platygastridae, Botany, Animals, Integrative taxonomy, DNA barcoding, Mealybug, Brazil
Abstract: Parasitoids of three mealybug pests (Hemiptera: Pseudococcidae), Planococcus ficus (Signoret), Pseudococcus sociabilis Hambleton, and Pseudococcus viburni (Signoret) have been identified for the first time in Brazil. Mealybugs were collected in fruit-growing areas along southern Brazil during 2013-2016. An integrative approach, combining morphological and molecular methods, was used to identify the Brazilian parasitoids to the species level. Fifteen species were recorded, including 14 primary parasitoids belonging to Encyrtidae and Platygastridae and a single secondary parasitoid species belonging to Signiphoridae. The encyrtid parasitoids Acerophagus flavidulus (Brethes), Anagyrus calyxtoi Noyes and Zaplatycerus sp., and the signiphorid secondary parasitoid Chartocerus axillaris De Santis are reported for the first time in Brazil., Centro de Estudios Parasitológicos y de Vectores, Centro de Investigación en Sanidad Vegetal
Published: 2021
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11. Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit

Author: de Melo, Bruno Paes, Lourenço-Tessutti, Isabela Tristan, Paixão, Joaquin Felipe Roca, Noriega, Daniel David, Silva, Maria Cristina Mattar, Fontes, Elizabeth Pacheco Batista, Grossi-de-Sa, Maria Fatima, De Melo, Bruno, Lourenço-Tessutti, Isabela, Paixão, Joaquin, Noriega, Daniel, Silva, Maria, de Almeida-Engler, Janice, Fontes, Elizabeth, Grossi-De-Sa, Maria, Brazilian Agricultural Research Corporation (Embrapa), Universidade Federal de Vicosa (UFV), Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Universidade Católica de Brasília (UCB), National Institute of Science and Technology (INCT), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), INCT PlantStress Biotech, UCB Pharma SA, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Apoio a Pesquisa do Distrito Federal (FAPDF), Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), BRUNO PAES DE MELO, UFV, ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen, JOAQUIN FELIPE ROCA PAIXÃO, UFRJ, DANIEL DAVID NORIEGA, UCB, MARIA CRISTINA MATTAR DA SILVA, Cenargen, JANICE DE ALMEIDA-ENGLER, UMR INSTITUT SOPHIA AGROBITECH INRA/CNRS/UNS, FRANCE, ELIZABETH PACHECO BATISTA FONTES, UFV, and MARIA FATIMA GROSSI DE SA, Cenargen.
Subjects: 0106 biological sciences, 0301 basic medicine, Transgene, [SDV]Life Sciences [q-bio], Arabidopsis, lcsh:Medicine, 01 natural sciences, CRISPRa, 03 medical and health sciences, Gene Expression Regulation, Plant, CRISPR-Associated Protein 9, Gene expression, lcsh:Science, AREB-1, Gene, Water deficit, Plant Physiological Phenomena, 2. Zero hunger, Regulation of gene expression, Gene Editing, Multidisciplinary, biology, Dehydration, Arabidopsis Proteins, fungi, lcsh:R, food and beverages, Histone acetyltransferase, Drought tolerance, 15. Life on land, biology.organism_classification, Plants, Genetically Modified, Chromatin, Cell biology, 030104 developmental biology, Basic-Leucine Zipper Transcription Factors, Genetic engineering, [SDE]Environmental Sciences, biology.protein, lcsh:Q, Adaptation, CRISPR-Cas Systems, 010606 plant biology & botany
Abstract: Plants are sessile organisms, which are vulnerable to environmental stresses. As such, plants have developed multiple molecular, physiological, and cellular mechanisms to cope with natural stressors. However, these environmental adversities, including drought, are sources of the main agribusiness problems since they interfere with plant growth and productivity. Particularly under water deprivation conditions, the abscisic acid-responsive element-binding protein AREB1/ABF2 plays an important role in drought stress response and physiological adaptation. In this investigation, we provide substantial confirmation for the role of AREB1/ABF2 in plant survival under severe water deficit using the CRISPR activation (CRISPRa) technique to enhance the AREB1 gene expression. In our strategy, the inactive nuclease dCas9 was fused with an Arabidopsis histone acetyltransferase 1, which improves gene expression by remodeling chromatin. The AREB1 overexpression promotes an improvement in the physiological performance of the transgenic homozygous plants under drought, which was associated with an increase in chlorophyll content, antioxidant enzyme activity, and soluble sugar accumulation, leading to lower reactive oxygen species accumulation. Finally, we found that the CRISPR-mediated up-regulation of AREB1 changes the abundance of several downstream ABA-inducible genes, allowing us to report that CRISPRa dCas9-HAT is a valuable biotechnological tool to improve drought stress tolerance through the positive regulation of AREB1.
Published: 2020
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12. Integrative taxonomy of Meloidogye ottersoni (Thorne, 1969) Franklin, 1971 (Nematoda: Meloidogynidae) parasitizing flooded rice in Brazil

Author: Leite, Raycenne Rosa, Gomes, Ana Cristina M. M., Py, Leandro Grimaldi, Leite, Raycenne, Mattos, Vanessa, Gomes, Ana, Py, Leandro, Souza, Daniela, Castagnone-Sereno, Philippe, Cares, Juvenil, Carneiro, Regina, Universidade de Brasilia [Brasília] (UnB), Embrapa Recursos Genéticos e Biotecnologia [Brasília], Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Interactions plantes-microorganismes et santé végétale (IPMSV), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), and Embrapa Genetic Resources and Biotechnology and Research Foundation of the Federal District (FAPDF) 193.001.167/2015
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, 0106 biological sciences, 0301 basic medicine, root-knot nematode, [SDV]Life Sciences [q-bio], Population, Plant Science, Horticulture, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, 01 natural sciences, 03 medical and health sciences, Graminicola, morphology, Botany, D2D3, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, education, Canary grass, COXII, education.field_of_study, Oryza sativa, biology, esterase phenotyping, Oryza, Parthenogenesis, Phalaris arundinacea, biology.organism_classification, 030104 developmental biology, Nematode, Taxonomy (biology), ITS, Agronomy and Crop Science, 010606 plant biology & botany
Abstract: International audience; A root-knot nematode (RKN) parasitizing rice (Oryza sativaL.) and causing damage in Santa Catarina (SC), Rio Grande do Sul (RS) and Parana (PR) states (Brazil) was identified asMeloidogyne ottersoni(Thorne1969) Franklin1971. The species is redescribed from the Brazilian population from Meleiro (SC) and compared with the description ofM. ottersonifrom Wind Lake (Wisconsin, USA) with additional morphological, biochemical and molecular characterization. The female and male bear smaller stylets: 10-12 mu m, 14-16 mu m, respectively, when compared withM. graminicola: 12-14 mu m, 16-18 mu m, andM. oryzae: 14-16 mu m, 18-20 mu m.Meloidogyne ottersonipresents perineal patterns located on the contour of a slight protuberance. Striae are mostly continuous, never raised by transverse irregular striae, as frequently observed inM. graminicolaandM. oryzae.Meloidogyne ottersonibelongs to the RKN group 11 described by Jepson (1987); the reproduction is by meiotic parthenogenesis and the somatic chromosome number is 18. The tail of second-stage juveniles is very long and thin, and tapers to a long, narrow, irregular hyaline terminus (M. ottersoni, 20.5 mu mvs M. graminicola,17.9 mu m andM. oryzae, 22.0 mu m, respectively). The ability of the BrazilianM. ottersonipopulation to parasitize canary grass,Phalaris arundinaceaL. (type host), and barnyard grass,Echinocloa crus-galli,was confirmed. Biochemically, the esterase profile ofM. ottersonilacks any band (Est Ot0, Rm=0), which differentiates it fromM. graminicolaandM. oryzae(Est VS1, Rm=0.70 and Est O1,Rm=1.02, respectively). In Maximum Likelihood analysis of ITS, D2D3 and COXII-16S rRNA sequences, populations ofM. ottersonifrom different states of Brazil clustered together and were separated from otherMeloidogynespp., thus confirming that all four populations are very similar and conspecific.
Published: 2020
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13. Shut down of the South American summer monsoon during the penultimate glacial

Author: Rodríguez-Zorro, Paula A., Ledru, Marie-Pierre, Bard, Edouard, Aquino-Alfonso, Olga, Camejo, Adriana, Daniau, Anne-Laure, Favier, Charly, Garcia, Marta, Mineli, Thays D., Rostek, Frauke, Ricardi-Branco, Fresia, Sawakuchi, André Oliveira, Simon, Quentin, Tachikawa, Kazuyo, Thouveny, Nicolas, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD), Chaire Evolution du climat et de l'océan, Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Collège de France (CdF (institution))-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Collège de France (CdF (institution))-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), University of Campinas [Campinas] (UNICAMP), Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Collège de France (CdF (institution))-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Universidade de São Paulo (USP), University of Campinas (UNICAMP), Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)BIOTA 2013/50297-0National Science Foundation (NSF)DEB 1343578Foundation BNP PARIBASCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)304727/2017-2303527/2017-0, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Collège de France - Chaire Evolution du climat et de l'océan, Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universidade Estadual de Campinas = University of Campinas (UNICAMP), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Universidade de São Paulo = University of São Paulo (USP)
Subjects: [SDU.STU.CL]Sciences of the Universe [physics]/Earth Sciences/Climatology, [SDE.MCG]Environmental Sciences/Global Changes, lcsh:R, Palaeoecology, lcsh:Medicine, lcsh:Q, Palaeoclimate, lcsh:Science, Article
Abstract: International audience; We analysed changes in mean annual air temperature (MAAt), vegetation and biomass burning on a long and continuous lake-peat sediment record from the colônia basin, southeastern Brazil, examining the responses of a wet tropical rainforest over the last 180 ka. Stronger southern atmospheric circulation up to the latitude of colônia was found for the penultimate glacial with lower temperatures than during the last glacial, while strengthening of the South American summer monsoon (SASM) circulation started during the last interglacial and progressively enhanced a longer wet summer season from 95 ka until the present. Past MAAT variations and fire history were possibly modulated by eccentricity, although with signatures which differ in average and in amplitude between the last 180 ka. Vegetation responses were driven by the interplay between the SASM and southern circulation linked to Antarctic ice volume, inferred by the presence of a cool mixed evergreen forest from 180 to 45 ka progressively replaced by a rainforest. We report cooler temperatures during the marine isotope stage 3 (MIS 3: 57-29 ka) than during the Last Glacial Maximum (LGM: 23–19 ka). Our findings show that tropical forest dynamics display different patterns than mid-latitude during the last 180 ka.
Published: 2020
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14. What is the influence of anthropogenic impact on the population structure of Attalea speciosa Mart. ex Spreng. in the Brazilian Amazonian region?

Author: Alessio Moreira dos Santos, Danielle Mitja, Izildinha de Souza Miranda, Patrice Loisel, Eric Delaître, Laurent Demagistri, Universidade Federal Rural da Amazônia (UFRA), UMR 228 Espace-Dev, Espace pour le développement, Institut de Recherche pour le Développement (IRD)-Université de Perpignan Via Domitia (UPVD)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Guyane (UG)-Université des Antilles (UA)-Université de Montpellier (UM), Mathématiques, Informatique et STatistique pour l'Environnement et l'Agronomie (MISTEA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), RELAIS (CNPq/IRD/UFRJ), CNES TOSCA/Babacu projects 130318, and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) 202583/2014-7
Subjects: anthropogenic impact, [SDV]Life Sciences [q-bio], population structure, Plant Science, Amazon, Attalea speciosa, [SHS]Humanities and Social Sciences
Abstract: International audience; Attalea speciosa (babassu) is a native palm of the primary forest from Amazonian and Cerrado biomes, and has multiple economic and cultural uses. However, this palm can become dominant in open areas, with a long-term persistence in the community. The objective of this study was to compare the population structure and morphology of babassu in three habitat types characterizing forest succession: primary forest, pasture, and babaçual (babassu-dominated secondary forest). For this purpose, we monitored 6,333 individuals for three years at six life stages in 11 sites with 25 plots located in PA-Benfica, Itupiranga-Pará, Brazil. The morphological parameters showed differences between secondary environments (pasture and babaçual) and primary forests, suggesting that this species has a high capacity for phenotypic plasticity. The inverse J-shaped distribution was observed only in primary forests, with the density of all stages constant along the whole study, unlike pastures and babaçual areas. While the density of seedlings is highest in primary forests, stage 4 and 5 juveniles and adults are most numerous in babaçuals. Our results suggest that the higher dominance of A. speciosa in babaçual areas can be associated with the resilience of this species to anthropogenic disturbances.
Published: 2022
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15. Lifestyle changes and mental health during the COVID-19 pandemic: A repeated, cross-sectional web survey

Author: Susana Gomes-da-Costa, Cristina Esteban, Beatriz Atienza-Carbonell, Eduard Vieta, Rafael Tabarés-Seisdedos, María Paz García-Portilla, Vicent Balanzá-Martínez, Iñaki Zorrilla, Benedicto Crespo-Facorro, Ana González-Pinto, M. Jose Jaen-Moreno, Raquel Brandini De Boni, Fernando Sarramea, Jose Cervera-Martínez, Teresa Bobes-Bascarán, Jurema Corrêa da Mota, Flávio Kapczinski, [Cervera-Martinez, Jose] Hosp Denia Marina Salud, Alicante, Spain, [Cervera-Martinez, Jose] Univ Valencia, Dept Med, Valencia, Spain, [Atienza-Carbonell, Beatriz] Univ Valencia, Dept Med, Valencia, Spain, [Mota, Jurema C.] Oswaldo Cruz Fdn FIOCRUZ, Inst Sci & Technol Commun & Informat Hlth ICICT, Rio De Janeiro, Brazil, [De Boni, Raquel B.] Oswaldo Cruz Fdn FIOCRUZ, Inst Sci & Technol Commun & Informat Hlth ICICT, Rio De Janeiro, Brazil, [Bobes-Bascaran, Teresa] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Crespo-Facorro, Benedicto] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Paz Garcia-Portilla, Maria] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Gonzalez-Pinto, Ana] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Sarramea, Fernando] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Vieta, Eduard] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Zorrilla, Inaki] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Tabares-Seisdedos, Rafael] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Balanza-Martinez, Vicent] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain, [Bobes-Bascaran, Teresa] Inst Invest Sanitaria Principado Asturias ISPA, Serv Salud Principado Asturias SESPA, Oviedo, Spain, [Paz Garcia-Portilla, Maria] Inst Invest Sanitaria Principado Asturias ISPA, Serv Salud Principado Asturias SESPA, Oviedo, Spain, [Bobes-Bascaran, Teresa] Univ Oviedo, Dept Psychol, Oviedo, Spain, [Crespo-Facorro, Benedicto] Hosp Univ Virgen del Rocio, IBIS, Seville, Spain, [Esteban, Cristina] Hosp Univ Virgen del Rocio, IBIS, Seville, Spain, [Crespo-Facorro, Benedicto] Univ Seville, Seville, Spain, [Paz Garcia-Portilla, Maria] Univ Oviedo, Sch Med, Dept Psychiat, Oviedo, Spain, [Gomes-da-Costa, Susana] Univ Barcelona, Hosp Clin, Inst Neurosci, IDIBAPS, Barcelona, Catalonia, Spain, [Vieta, Eduard] Univ Barcelona, Hosp Clin, Inst Neurosci, IDIBAPS, Barcelona, Catalonia, Spain, [Gonzalez-Pinto, Ana] Univ Basque Country, Hosp Univ Alava BIOARABA, Vitoria, Spain, [Zorrilla, Inaki] Univ Basque Country, Hosp Univ Alava BIOARABA, Vitoria, Spain, [Jaen-Moreno, M. Jose] Inst Maimonides Invest Biomed Cordoba IMIBIC, Cordoba, Spain, [Sarramea, Fernando] Inst Maimonides Invest Biomed Cordoba IMIBIC, Cordoba, Spain, [Jaen-Moreno, M. Jose] Univ Cordoba, Dept Ciencias Morfol & Sociosanitarias, Cordoba, Spain, [Sarramea, Fernando] Univ Cordoba, Hosp Univ Reina Sofia, Cordoba, Spain, [Tabares-Seisdedos, Rafael] Univ Valencia, Dept Med, Teaching Unit Psychiat & Psychol Med, Blasco Ibanez 15, Valencia 46010, Spain, [Balanza-Martinez, Vicent] Univ Valencia, Dept Med, Teaching Unit Psychiat & Psychol Med, Blasco Ibanez 15, Valencia 46010, Spain, [Kapczinski, Flavio] McMaster Univ, Dept Psychiat & Behav Neurosci, Mood Disorders Program, St Josephs Healthcare Hamilton, Hamilton, ON, Canada, [Kapczinski, Flavio] Univ Fed Rio Grande Sul UFRGS, Inst Nacl Ciencia & Tecnol Translac Med INCT TM, Dept Psychiat, Porto Alegre, RS, Brazil, Instituto de Salud Carlos III, ISCIII, Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Spanish Ministry of Science and Innovation, Plan Nacional de I+D+I - Fondo Europeo de Desarrollo Regional (FEDER), Instituto de Salud Carlos III, CIBER of Mental Health (CIBERSAM), Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement, CERCA Programme, and Departament de Salut de la Generalitat de Catalunya for the PERIS grant
Subjects: Web survey, Lifestyle Mental health, Population, Alcohol abuse, Anxiety, External validity, Social support, Self-rated health, Surveys and Questionnaires, Pandemic, Lockdown, medicine, Humans, Life Style, Pandemics, Exercise, Depression (differential diagnoses), Psychiatry, Resilience, business.industry, SARS-CoV-2, Depression, Prevention, COVID-19, General population, medicine.disease, Lifestyle, Mental health, Psychiatry and Mental health, Clinical Psychology, Cross-Sectional Studies, Mental Health, Scale (social sciences), Communicable Disease Control, medicine.symptom, business, Sleep, Demography, Research Paper
Abstract: VBM acknowledges the national grant PI16/01770 from the Instituto de Salud Carlos III, ISCIII (The PROBILIFE study). EV thanks the support of the Spanish Ministry of Science and Innovation (PI15/00283, PI18/00805) integrated into the Plan Nacional de I+D+I and co-financed by the ISCIII-Subdirección General de Evaluación and the Fondo Europeo de Desarrollo Regional (FEDER); the Instituto de Salud Carlos III; the CIBER of Mental Health (CIBERSAM) (...), Cervera-Martínez, J., Atienza-Carbonell, B., Mota, J.C., Bobes-Bascarán, T., Crespo-Facorro, B., Esteban, C., García-Portilla, M.P., Gomes-da-Costa, S., González-Pinto, A., Jaén-Moreno, M.J., Sarramea, F., Vieta, E., Zorrilla, I., Tabarés-Seisdedos, R., Kapczinski, F., De Boni, R.B., Balanzá-Martínez, V.
Published: 2021

16. Gradient of decomposition in sugarcane mulches of various thicknesses

Author: Guilherme Dietrich, Adriane Luiza Schu, Douglas Adams Weiler, Sandro José Giacomini, Patrick Leal Pinheiro, Mathias Roberto Leite Rambo, Sylvie Recous, Soils Department, Universidade Federal de Santa Maria (UFSM), Fractionnement des AgroRessources et Environnement - UMR-A 614 (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Brazil government through the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil government through the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Institut National de la Recherche Agronomique (INRA) - Environment & Agronomy Division, Program CNPq-Ciencia sem Fronteiras 208415/2017-3, Fractionnement des AgroRessources et Environnement (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), and Universidade Federal de Santa Maria = Federal University of Santa Maria [Santa Maria, RS, Brazil] (UFSM)
Subjects: Crop residue, Materials science, [SDV]Life Sciences [q-bio], décomposition, Soil Science, chemistry.chemical_element, engineering.material, sugarcane, paillis, pluviométrie, Dry matter, Water content, Chemical composition, Earth-Surface Processes, 2. Zero hunger, mulch, Brasil, 04 agricultural and veterinary sciences, dynamique de l'eau, 15. Life on land, Straw, Nitrogen, chemistry, Agronomy, crop residue, 040103 agronomy & agriculture, engineering, rainfall rate recording, 0401 agriculture, forestry, and fisheries, Fertilizer, straw mulches, Agronomy and Crop Science, Mulch, canne à sucre, évaporation
Abstract: Crop residues left on the soil surface as mulch influence many services provided in agrosystems, particularly soil protection, water dynamics and nutrient fluxes. For a given crop, the residue mass influences the mulch thickness, but the effect of thickness on decomposition is not well understood, and decomposition gradients within mulches in the field have not been described. This study aimed to quantify the decomposition of sugarcane straw mulches by varying their mass and thickness and determine the decomposition gradient within the mulches. The experiment was conducted on a first-ratoon sugarcane crop for one year, with 4, 8 and 12 Mg straw dry matter ha(-1). Mulches were displayed in 0.16-m(2) litter boxes and formed by straw layers of equivalent mass, i.e., 4 Mg ha(-1), stacked either as a single layer (Low/Top layer), 2 layers (Low and Top layers) or 3 layers (Low, Middle and Top layers) to reconstitute the 3 mulch quantities. We quantified the carbon (C) and nitrogen (N) contents, the chemical composition and the water content of the remaining mulch particles in each layer 10 times within the 360 days. The mulch degradation rates were proportional to the initial amount of straw, the mulch losses representing 75% of its initial C and 46% of its initial N after one year, regardless of the initial mass. Fertilizer-N addition did not change the k decomposition rate, with k = 0.0044 d(-1). However the decomposition rate differed according to the layer position in the mulch, e.g., in the 12 Mg ha(-1) treatment k = 0.0064 d(-1) 0.0046 d(-1) and 0.0033 d(-1) for the low, middle and top layers, respectively, indicating that the low layers in contact with the soil decomposed faster than the top layers in contact with the atmosphere, while the medium layers demonstrated intermediate behaviour. The top layers evolved similarly, regardless of mulch thickness, while the lower layers in contact with the soil decomposed more quickly as the overlying straw thickness increased. Regardless of the thickness and position of a given layer within mulch, the one-year layer C loss was linearly correlated with the average water content of that layer over the year (y = 52.79 - 0694x, R-2 = 0.991, P < 0.05). Here, we demonstrate that the dynamics of mulch decomposition is primarily driven by the dynamics of water, which is itself driven by mulch characteristics, rain regime and evaporation.
Published: 2019
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17. Proteome dataset of Hemileia vastatrix by LC–MS/MS label-free identification

Author: Natália Faustino Cury, Daiane Gonzaga Ribeiro, Jonathan Dias de Lima, Pollyana da Nóbrega Mendes, Diana Fernandez, Wagner Fontes, Mariana S. Castro, Marcelo V. Sousa, Natália F. Martins, Angela Mehta, Brazilian Agricultural Research Corporation (Embrapa), Universidade Católica de Brasília=Catholic University of Brasília (UCB), Universidade de Brasilia [Brasília] (UnB), Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Bayer (Grants for Targets) (Project code 20.21.0 0.042.0 0.00), and Embrapa Recursos Geneticos e Biotecnologia, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Capes), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Institut de Recherche pour le Developpement (IRD) and CONCAFE (Project code 10.18.20.001.00.00)
Subjects: Multidisciplinary, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
Abstract: International audience; Here we describe the proteome of the fungus Hemileia vas-tatrix by label free mass spectrometry (LC-MS/MS). H. vas-tatrix is the causal agent of coffee rust disease, causing great economic losses in this crop. The objective of our work was to identify H. vastatrix proteins potentially in-volved in host colonization and infection, by exploring the shotgun proteomics approach. A total of 742 proteins were identified and are associated with several crucial molecu-lar functions, biological processes, and cellular components. The proteins identified contribute to a better understand-ing of the metabolism of the fungus and may help identify target proteins for the development of specific drugs in or-der to control coffee rust disease. All data can be accessed at the Centre for Computational Mass Spectrometry - Mas-sIVE MSV0 0 0 087665 -https://massive.ucsd.edu/ProteoSAFe/ dataset.jsp?task=cc71ad75f767451abe72dd1ce0019387
Published: 2022
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18. Click dendrimer-Pd nanoparticle assemblies as enzyme mimics: catalytic o-phenylenediamine oxidation and application in colorimetric H2O2 detection

Author: Yue Liu, Renata Pereira Lopes, Desirè Di Silvio, Tanja Lüdtke, Jean-René Hamon, Sergio Moya, Didier Astruc, Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Universidade Federal de Vicosa (UFV), Centro de Investigación Cooperativa en Biomateriales (CIC biomaGUNE), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), China Scholarship Council, CAPES/Brazil Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [88881.337360/2019-01], CNPq/FAPEMIG Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [SICONV: 793988/2013], Ministerio de Economia, Industria y Competitividad, gobierno de Espana [MAT2017-88752-R], University of Bordeaux, University of Rennes 1, Centre National de la Recherche Scientifique (CNRS), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Universidade Federal de Viçosa = Federal University of Viçosa (UFV), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Detection limit, Chemistry, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Combinatorial chemistry, Nanomaterial-based catalyst, Coupling reaction, 0104 chemical sciences, Catalysis, Inorganic Chemistry, chemistry.chemical_compound, Dendrimer, o-Phenylenediamine, [CHIM]Chemical Sciences, 0210 nano-technology, Triethylene glycol
Abstract: International audience; Dendrimers have already been successfully used in nanoparticle (NP) catalysis for many years, particularly for Pd NP protection towards carbon-carbon coupling reactions. In this paper, assemblies between Pd nanoparticles and two generations of "click" dendrimers, with 27 (dendrimer-1) and 81 (dendrimer-2) triethylene glycol (TEG) termini, respectively, are examined for catalytic peroxidase-like oxidative activity. This catalysis is investigated with o-phenylenediamine (OPD) and H2O2 as the substrates in water, displaying different colours. The dendritic effect is negative upon increasing generation, i.e., dendrimer 1-Pd nanoparticles show the best results, providing values with a V-max of 1.49 x 10(-9) M s(-1) and K-m of 3.02 mM as obtained with the Michaelis-Menten model. The detection limit is 0.5 mu M of H2O2 detection with dendrimer-1-PdNPs, and both dendrimer-Pd nanocatalysts exhibit excellent robustness of catalytic activity, with a water-dispersive state being stable for at least 2 months, thus showing promise as a mimic for peroxidase catalysts.
Published: 2021
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19. Effects of packaging color on expected flavor, texture, and liking of chocolate in Brazil and France

Author: Jorge Herman Behrens, Dominique Valentin, Iuri Yudi Furukita Baptista, Erick Saldaña, Universidade Estadual de Campinas (UNICAMP), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Bourgogne Franche-Comté [COMUE] (UBFC), Universidad Nacional de Moquegua (UNAM), and The Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) (140397/20183) and the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Capes) (88887.468115/2019-00).
Subjects: Cultural Studies, Taste, 030309 nutrition & dietetics, packaging, Dark chocolate, Texture (music), perception, taste, 03 medical and health sciences, 0404 agricultural biotechnology, food, cross-cultural, experience, Food science, Flavor, Aroma, Mathematics, 0303 health sciences, biology, chocolate, 04 agricultural and veterinary sciences, consumer perception, Sweetness, [SHS.ANTHRO-SE]Humanities and Social Sciences/Social Anthropology and ethnology, biology.organism_classification, 040401 food science, food.food, color, Milk Chocolate, visual cues, correspondences, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science, expectations
Abstract: International audience; Recent research has shown that the colors of plateware, glassware, cups, packaging, and even of the ambient and its lighting are able to influence consumers' preferences, expectations, and perceptions of taste, aroma, texture, and liking of food and beverages. This study contributes to the subject by investigating how packaging colors affect the expectations of sweetness, bitterness, fruitiness, melting, and liking for chocolates in Brazil and France. Two groups of 210 consumers (N = 420), one from each country, evaluated samples of milk and dark chocolate packaged in seven colors: black, blue, brown, green, red, pink, and yellow. Analyses of variance (ANOVA) and Tukey-Kramer's test indicated that there were multiple significant effects of packaging color on consumers' expectations. Multiple factor analysis (MFA) showed that expected sweetness, fruitiness, and liking were correlated to each other and inversely correlated to expected bitterness. While both chocolates were expected the least sweet and the most bitter when in black packaging, they were expected the sweetest and the least bitter when in yellow or pink packaging. Interestingly, the same black packaging made the milk chocolate the best rated and the dark chocolate the worst rated on expected liking, showing that a packaging color may have inverse effects on hedonic ratings depending on the type of chocolate. Although French consumers eat more chocolate and with higher cocoa content than Brazilians, the effects of packaging color were not significantly different between cultures.
Published: 2021
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20. Efficiency Enhancement in Mid-Range RWPT Systems by GRIN Metasurface Lenses

Author: Úrsula do Carmo Resende, Felipe M. de Freitas, Icaro V. Soares, Institut d'Électronique et des Technologies du numéRique (IETR), Université de Nantes (UN)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Centro Federal de Educação Tecnológica de Minas Gerais (CEFET-MG), FAPEMIG Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), CAPES Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), CNPq Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), CEFET-MG, Nantes Université (NU)-Université de Rennes 1 (UR1), Université de Nantes (UN)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, business.industry, Metamaterial, Physics::Optics, Topology (electrical circuits), ceramics, law.invention, Lens (optics), Split-ring resonator, [SPI]Engineering Sciences [physics], Planar, Optics, law, coaxial resonators, delay filters, Gradient-index optics, Wireless power transfer, power amplifiers, business, Refractive index, delay lines
Abstract: International audience; The Magnetic Resonant Wireless Power Transfer (RWPT) is one of the best suitable techniques for mid-range applications. Therefore, there are different approaches to enhance RWPT efficiency, many of them based on metamaterial devices. In this work, the GRadient-INdex (GRIN) technique is applied to increase the coupling between transmitter and receiver coils and collimate the magnetic field, reducing its dispersion. This proposed lens topology operates at 28 MHz and is formed by a 5x5 planar and periodic arrangement of Split Ring Resonators (SRR) unit cells. In each one, a lumped capacitor is added to reduce the lens's size and radially create a refractive index gradient. Then, a four-coil RWPT system is designed at the same operating frequency. Finally, the coils and lenses are prototyped, and the RWPT efficiency is measured in three cases: without any lenses, with a conventional (uniform) metasurface, and with the proposed GRIN lens. The experimental results demonstrate that the RWPT efficiency, which is about 8% without any lenses, becomes almost four times higher (33%) after the inclusion of metasurface GRIN lenses. When the same measurement is carried out with a uniform lens, typical topology in literature, the obtained efficiency is 17.59%, nearly half of the obtained with the proposed metasurface.
Published: 2021
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21. Defining the combined stress response in wild Arachis

Author: Ana Cristina Miranda Brasileiro, Maria Fatima Grossi-de-Sa, Mario Alfredo de Passos Saraiva, Ana Paula Zotta Mota, Bruna Vidigal, R. C. Togawa, Patricia Messenberg Guimaraes, Andressa da Cunha Quintana Martins, Thais Nicolini Oliveira, Ana Claudia Guerra de Araújo, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Embrapa Recursos Genéticos e Biotecnologia [Brasília], INCT Plant Stress Biotech, Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), UCB Pharma SA, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Apoio a Pesquisa do Distrito Federal (FAPDF), ANA PAULA ZOTTA MOTA, UFRGS, ANA CRISTINA MIRANDA BRASILEIRO, Cenargen, BRUNA VIDIGAL, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, THAIS NICOLINI OLIVEIRA, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, ANDRESSA DA CUNHA QUINTANA MARTINS, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, MARIO ALFREDO DE PASSOS SARAIVA, Cenargen, ANA CLAUDIA GUERRA DE ARAUJO, Cenargen, ROBERTO COITI TOGAWA, Cenargen, MARIA FATIMA GROSSI DE SA, Cenargen, and PATRICIA MESSEMBERG GUIMARAES, Cenargen.
Subjects: 0106 biological sciences, 0301 basic medicine, Meloidogyne Arenaria, Candidate gene, Arachis, [SDV]Life Sciences [q-bio], Science, Molecular engineering in plants, Wild Arachis, 01 natural sciences, Article, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Stress, Physiological, Arabidopsis, Animals, Tylenchoidea, Transcriptomics, Biotic, Abscisic acid, Gene, Disease Resistance, Plant Diseases, 2. Zero hunger, Genetics, Abiotic component, Multidisciplinary, Abiotic, biology, fungi, food and beverages, biology.organism_classification, Droughts, 030104 developmental biology, chemistry, Meloidogyne arenaria, Medicine, Ethylene hormone, 010606 plant biology & botany
Abstract: Nematodes and drought are major constraints in tropical agriculture and often occur simultaneously. Plant responses to these stresses are complex and require crosstalk between biotic and abiotic signaling pathways. In this study, we explored the transcriptome data of wild Arachis species subjected to drought (A-metaDEG) and the root-knot nematode Meloidogyne arenaria (B-metaDEG) via meta-analysis, to identify core-stress responsive genes to each individual and concurrent stresses in these species. Transcriptome analysis of a nematode/drought bioassay (cross-stress) showed that the set of stress responsive DEGs to concurrent stress is distinct from those resulting from overlapping A- and B-metaDEGs, indicating a specialized and unique response to combined stresses in wild Arachis. Whilst individual biotic and abiotic stresses elicit hormone-responsive genes, most notably in the jasmonic and abscisic acid pathways, combined stresses seem to trigger mainly the ethylene hormone pathway. The overexpression of a cross-stress tolerance candidate gene identified here, an endochitinase-encoding gene (AsECHI) from Arachis stenosperma, reduced up to 30% of M. incognita infection and increased post-drought recovery in Arabidopsis plants submitted to both stresses. The elucidation of the network of cross-stress responsive genes in Arachis contributes to better understanding the complex regulation of biotic and abiotic responses in plants facilitating more adequate crop breeding for combined stress tolerance.
Published: 2021
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22. Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet

Author: Adriano G. Cruz, Marciane Magnani, Matthaws Pereira de Oliveira, Whyara Karoline Almeida da Costa, Jailane de Souza Aquino, Valdir A. Braga, José Luiz de Brito Alves, Tatiana Colombo Pimentel, Larissa Ramalho Brandão, Melline F. Noronha, Hubert Vidal, Lucélia Cabral, Georgianna de Araújo Henriques Ferreira, Univ Fed Paraiba, Fed Inst Sci & Technol Rio De Janeiro, Univ Claude Bernard Lyon 1, Univ Illinois, Universidade Estadual Paulista (Unesp), Fed Inst Parana, Universidade Federal da Paraiba (UFPB), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lyon, Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon, University of Illinois [Chicago] (UIC), University of Illinois System, Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Instituto Federal Paraná (IFPR), and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)303437/2017-0405644/2018-3'Coordenacao de Aconselhamento de Pessoal de Nivel Superior' (CAPES-Brazil)001UFPB PrInt 88881.311776/2018-01Fundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQPB)007/2019 FAPESQ-PB-MCT/CNPq
Subjects: 0301 basic medicine, medicine.medical_specialty, Biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Insulin resistance, Internal medicine, medicine, Ingestion, Clostridiaceae, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, 2. Zero hunger, Cholesterol, Lachnospiraceae, digestive, oral, and skin physiology, food and beverages, General Medicine, biology.organism_classification, medicine.disease, Enterobacteriaceae, 3. Good health, 030104 developmental biology, Endocrinology, Blood pressure, chemistry, 030221 ophthalmology & optometry, Bacteria, Food Science
Abstract: Made available in DSpace on 2021-06-25T15:05:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-08 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQ) This study aimed to evaluate the effects of ingestion of live (9 log CFU mL(-1)) and ultrasound-inactivated (paraprobiotic, 20 kHz, 40 min) Lacticaseibacillus casei 01 cells for 28 days on healthy parameters (biochemical and cardiovascular) and intestinal microbiota (amplicon sequencing of 16S ribosomal RNA) of rats fed a high-fat diet. Twenty-four male Wistar rats were divided into four groups of six animals: CTL (standard diet), HFD (high-fat diet), HFD-LC (high-fat diet and live L. casei), and HFD-ILC (high-fat diet and inactivated L. casei). The administration of live and ultrasound-inactivated L. casei prevented the increase (p < 0.05) in cholesterol levels (total and LDL) and controlled the insulin resistance in rats fed a high-fat diet. Furthermore, it promoted a modulation of the intestinal microbial composition by increasing (p < 0.05) beneficial bacteria (Lachnospiraceae and Ruminoccocaceae) and decreasing (p < 0.05) harmful bacteria (Clostridiaceae, Enterobacteriaceae, and Helicobacteriacea), attenuating the effects promoted by the HFD ingestion. Only live cells could increase (p < 0.05) the HDL-cholesterol, while only inactivated cells caused attenuation (p < 0.05) of the blood pressure. Results show beneficial effects of live and inactivated L. casei 01 and indicate that ultrasound inactivation produces a paraprobiotic with similar or improved health properties compared to live cells. Univ Fed Paraiba, Dept Food Engn, Technol, Joao Pessoa, Paraiba, Brazil Univ Fed Paraiba, Hlth Sci Ctr, Dept Nutr, Joao Pessoa, Paraiba, Brazil Fed Inst Sci & Technol Rio De Janeiro, Dept Food, Rio De Janeiro, RJ, Brazil Univ Fed Paraiba, Dept Biotechnol, Biotechnol Ctr, Joao Pessoa, Paraiba, Brazil Univ Claude Bernard Lyon 1, Univ Lyon, CarMeN Cardio Metab Diabet & Nutr Lab, INSERM,INRAE,INSA Lyon, Oullins, France Univ Illinois, Res Resource Ctr, Res Informat Core, Chicago, IL USA Sao Paulo State Univ, Dept Gen & Appl Biol, Inst Biosci, Rio Claro, SP, Brazil Fed Inst Parana, Campus Paranavai, Paranavai, Brazil Sao Paulo State Univ, Dept Gen & Appl Biol, Inst Biosci, Rio Claro, SP, Brazil CNPq: 303437/2017-0 CNPq: 405644/2018-3 CAPES: 001 CAPES: UFPB PrInt 88881.311776/2018-01 Fundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQ): 007/2019 FAPESQ-PB-MCT/CNPq
Published: 2021
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23. Mycorrhizal inoculation increases fruit production without disturbance of native arbuscular mycorrhizal community in jujube tree orchards (Senegal)

Author: Amadou Moustapha Bâ, Hervé Sanguin, Aboubacry Kane, Diaminatou Sanogo, Sergio Miana de Faria, Robin Duponnois, Cheikh Ndiaye, Babacar Thioye, Samba Ndao Sylla, Dioumacor Fall, UniLaSalle, Agro-écologie, Hydrogéochimie, Milieux et Ressources (AGHYLE), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut Sénégalais de Recherches Agricoles [Dakar] (ISRA), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Centre National de Recherches Forestières de l’Institut Sénégalais de Recherches Agricoles (CNRF), Brazilian Agricultural Research Corporation (Embrapa), Université des Antilles (UA), Africa-Brazil-France tripartite research project, AIRD, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), APGMV, Institut de Recherche pour le Developpement (IRD), Institut Senegalais de Recherches Agricoles (ISRA), French Embassy in Senegal (SCAC), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), and Plant Health Institute of Montpellier (UMR PHIM)
Subjects: 0106 biological sciences, 0301 basic medicine, amélioration des cultures, [SDV]Life Sciences [q-bio], Orchard, F62 - Physiologie végétale - Croissance et développement, Performance de culture, 01 natural sciences, Persistence (computer science), Cultivar, [SDV.SA.HORT]Life Sciences [q-bio]/Agricultural sciences/Horticulture, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, 2. Zero hunger, food and beverages, Ziziphus, Biofertilisant, Horticulture, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Fruit production, General Agricultural and Biological Sciences, F60 - Physiologie et biochimie végétale, Biofertilizer, Cultivars, Mycorhization, Biology, 03 medical and health sciences, Inoculation, F03 - Production et traitement des semences, Variété, Glomeromycota, Arbuscular mycorrhiza [EN], fungi, Sowing, Climat semi-aride, 15. Life on land, biology.organism_classification, Ziziphus mauritiana, 030104 developmental biology, Disturbance (ecology), Fruit tree, 010606 plant biology & botany
Abstract: International audience; Arbuscular mycorrhizal (AM) fungi constitute promising biofertilizers for the deployment of a sustainable agriculture. However, the evaluation of mycorrhizal benefits in the field is poorly documented, notably in fruit tree orchards, and concerns exclusively growth performance and nutrition. Here, we evaluated the persistence of beneficial effects of AM fungal inoculation on two jujube (Ziziphus mauritiana Lam.) cultivars (Tasset and Gola) inoculated in nursery conditions and subsequently transferred in the field until fruit production. The growth-promoting effects on jujube observed in nursery was still maintained 18 months after planting, with significant higher rates of survival and an increase of fruit production for inoculated jujube cultivars. Nevertheless, AM-mediated responses were dependent on cultivar. No major disturbance of native AM fungal community was associated with AM fungal inoculation but a stimulation of AM fungal colonization is probable. The use of mycorrhizal inoculation with the R. irregularis IR27 strain hold promise for the development of efficient jujube orchards under semi-arid conditions.
Published: 2021
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24. Multiscale analyses of growth and berry distributions along four branching orders and vertical profile of Coffea arabica L. cultivated under high-density planting systems

Author: Eliemar Campostrini, Fabio Takeshi Matsunaga, Danilo Força Baroni, Evelyne Costes, Miroslava Rakocevic, Agronomical Institute of Paraná (IAPAR), IAPAR, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Instituto SENAI de Tecnologia, Architecture et Fonctionnement des Espèces Fruitières [AGAP] (AFEF), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Brazilian Consorcio Pesquisa Cafe (02.09.20.008.00.00, 02.13.02.042.00.00), and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) 312959/2019-2
Subjects: 0106 biological sciences, 0301 basic medicine, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Yield, Planting pattern, Berry production, High density, Berry, Horticulture, 01 natural sciences, 03 medical and health sciences, Plant production, Metamer, Vertical profile, Axes order, Leaf area index, Mathematics, 2. Zero hunger, Planting density, Coffea arabica, Plant architecture, Sowing, 15. Life on land, LAI, 030104 developmental biology, Arabic coffee, 010606 plant biology & botany
Abstract: International audience; In horticulture, different planting designs can be used to optimize the plant production. In the coffee tree, we assumed that different planting densities and spatial patterns may impact the growth of all branching order axes, which in turn would impact the berry production. The aim of this study was to quantify and compare axes growth, leaf area, berry distribution and yield in four production years, depending on four planting designs. Experimental data were collected in the 1st, 2nd, 6th and 7th production years (PY), on Coffea arabica trees planted in two high densities (6000 or 10,000 plants ha(-1)) and planting patterns (square or rectangular). Coffee architecture was described from metamer to axis, layer, and plant scales. Metamer number per axis, axes number and cumulated length, together with berry distribution, were compared along 40 cm-thick layers of plant vertical profile, depending on the planting design. In the 1st and 2nd PY, plants were formed by two layers, with axes of 4th order appearing in layer 1. In the 2nd PY, the 3rd order axes which had a crucial role in total berry production, were about 3-4 folds more numerous than 2nd order axes in layer 1. In the 6th and 7th PY, the plants included five layers and five branching orders. The leaf area index, 2nd - 4th order axes length, berry distribution and yield were shown to depend on both planting design and PY. Both planting patterns under 10,000 plants ha(-1) and rectangular planting pattern under 6000 plants ha(-1) could be recommended for high production up to 7th PY, while the square planting pattern under 6000 plants ha(-1) must be pruned after 6th PY.
Published: 2021
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25. Comparative Proteomic Analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) Tropical and Temperate Lineages: Uncovering Differences During Ehrlichia canis Infection

Author: Margarita Villar, Gervásio Henrique Bechara, Joana Ferrolho, Joana Couto, Marcos Rogério André, Lourdes Mateos-Hernández, Rosangela Zacarias Machado, Isabel G. Fernández de Mera, José de la Fuente, Ana Domingos, Sandra Antunes, Gustavo Seron Sanches, Darci Moraes Barros-Battesti, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Portuguese Foundation for Science and Technology European CommissionPTDC/CVT-WEL/1807/2014GHTM - UID/Multi/04413/2013SFRH/BD/122894/2016SFRH/BD/121946/2016, Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) 2018/06651-7, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)421980/2016-8302420/2017-7, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)88887.464801/2019-00, Fundação para a Ciência e a Tecnologia (Portugal), Fundação de Amparo à Pesquisa do Estado de São Paulo, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Universidade Nova de Lisboa (GHTM-IHMT-UNL), Pontifícia Universidade Católica do Paraná, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ciudad Real, Universidade Estadual Paulista (Unesp), Université Paris-Est, Oklahoma State University, and École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
Subjects: 0301 basic medicine, Microbiology (medical), Ixodidae, Ehrlichia canis, Rhipicephalus sanguineus, Lineage (evolution), 030231 tropical medicine, Immunology, lcsh:QR1-502, Ehrlichia, Zoology, icks, sialome, Tick, Microbiology, lcsh:Microbiology, ticks, 03 medical and health sciences, Cellular and Infection Microbiology, Dogs, 0302 clinical medicine, proteomics, Animals, Dog Diseases, Original Research, vector competence, biology, Ehrlichiosis, biology.organism_classification, 030104 developmental biology, Infectious Diseases, Canis, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Vector (epidemiology), mialome
Abstract: The tick vector Rhipicephalus sanguineus is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the “temperate” and “tropical” lineages of R. sanguineus (s.l.) is the vector competence to Ehrlichia canis, suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the R. sanguineus tropical and temperate lineages and compares their proteomic profile during E. canis infection. Batches of nymphs from the two lineages were allowed to feed on naïve and experimentally E. canis infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of E. canis before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to E. canis infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial R. sanguineus systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control., This research was supported by the project PTDC/CVT-WEL/1807/2014, subsidized by the Fundação para a Ciência e Tecnologia (FCT). The authors would like to acknowledge FCT for funds to GHTM – UID/Multi/04413/2013, Fundação de Amparo a Pesquisa do Estado de São Paulo - FAPESP (2018/06651-7), Conselho Nacional de Desenvolvimento Cientı́fico e Tecnológico –CNPq (421980/2016-8) and Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior – CAPES (88887.464801/2019-00). MA is a fellowship of CNPq (Productivity Grant - Process #302420/2017-7). JF and JC are fellowships of FCT (Grants SFRH/BD/122894/2016 and SFRH/BD/121946/2016).
Published: 2021
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26. A Randomized Crossover Intervention Study on the Effect a Standardized Maté Extract (Ilex paraguariensis A. St.-Hil.) in Men Predisposed to Cardiovascular Risk

Author: Karimi S. Gebara, Arquimedes Gasparotto Junior, Carla I. Bonetti, Paula T. Gozzi, Christine Morand, Euclides Lara Cardozo Junior, Rhanany Alan Calloi Palozi, Telma Aparecida Costa, Martha R. F. de Mello, Federal University of Grande Dourados (UFGD), Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Universidade Paranaense, and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)200200/2012-7
Subjects: 0301 basic medicine, Blood Glucose, Male, Physiology, Blood Pressure, 0302 clinical medicine, caffeoyl quinic acids, education.field_of_study, Nutrition and Dietetics, Framingham Risk Score, Cross-Over Studies, cardiometabolic disease, clinical trial, Middle Aged, Lipids, 3. Good health, C-Reactive Protein, Cardiovascular Diseases, Population study, lcsh:Nutrition. Foods and food supply, Ilex paraguariensis, Population, 030209 endocrinology & metabolism, lcsh:TX341-641, Placebo, Article, 03 medical and health sciences, Double-Blind Method, chlorogenic acids, medicine, maté, Humans, education, extract, Aged, 030109 nutrition & dietetics, business.industry, Interleukin-6, Plant Extracts, medicine.disease, Crossover study, Blood pressure, Heart Disease Risk Factors, maté extract, Metabolic syndrome, business, fasting glucose, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Biomarkers, Food Science, Lipoprotein
Abstract: (1) Background: Due to its richness in chlorogenic acids (CGAs), Mat&eacute, (Ilex paraguariensis A. St.-Hil.) could be of interest in the prevention of cardiometabolic diseases, however clinical evidence are lacking. This trial aimed to evaluate the impact of mat&eacute, CGAs, consumed in a daily dose achievable through traditional mat&eacute, beverages, on parameters related to cardiometabolic risk. (2) Design: Thirty-four male volunteers aged 45&ndash, 65 years and with at most one criteria of metabolic syndrome, were recruited for a randomized, double-blind, placebo-controlled, and crossover study. The volunteers were assigned to consume an encapsulated dry mat&eacute, extract for four-weeks, providing 580 mg of caffeoyl quinic acid derivatives (CQAs) daily, or a placebo, with a two weeks washout between intervention periods. Anthropometric variables, blood pressure, plasma glucose, lipids, endothelial, and inflammatory biomarkers were measured in overnight-fasted subjects and after a glucose load. (3) Results: We found no significant effects of treatment on these parameters and the response to the glucose load was also similar between the two interventions. However, a significant decrease in fasting glucose was observed between day 0 and day 28 for the mat&eacute, group only (&minus, 0.57 &plusmn, 0.11 mmol/L, p &lt, 0.0002). In subjects with an intermediate to high Framingham risk score, consumption of mat&eacute, extract induced a 10% increase of high-density lipoprotein (HDL)-c from baseline. In a subgroup representative of the study population, significant decreases in the C-reactive protein (CRP) (&minus, 50%) and interleukin-6 (IL-6) (&minus, 19%) levels were observed. (4) Conclusions: These clinical observations suggest that mat&eacute, naturally rich in CGAs, could improve some cardiometabolic markers in subjects with a higher predisposition to metabolic syndrome, even if that remains to be confirmed in new trials specifically targeting this population.
Published: 2021
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27. Modeling of two CoRoT solar analogues constrained by seismic and spectroscopic analysis

Author: J P Marques, Frédéric Baudin, Othman Benomar, Matthieu Castro, Caroline Barban, J. S. da Costa, Patrick Boumier, J. D. do Nascimento, Thierry Morel, Y. Lebreton, R. Samadi, Univesidade Federal do Rio Grande do Norte, Institut d'astrophysique spatiale (IAS), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), New York University [Abu Dhabi], NYU System (NYU), National Astronomical Observatory of Japan (NAOJ), Laboratoire d'études spatiales et d'instrumentation en astrophysique (LESIA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université de Liège, Universidade Federal do Rio Grande do Norte [Natal] (UFRN), Harvard-Smithsonian Center for Astrophysics (CfA), Smithsonian Institution-Harvard University [Cambridge], Institut de Physique de Rennes (IPR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), CNPq (Bolsa de Produtividade)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), Belspo Belgian Federal Science Policy Office, Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Centre National d’Études Spatiales [Paris] (CNES), Harvard University-Smithsonian Institution, and Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)
Subjects: stars: abundances, Metallicity, FOS: Physical sciences, Astrophysics, Astrophysics::Cosmology and Extragalactic Astrophysics, asteroseismology, stars: interiors, 7. Clean energy, 01 natural sciences, Luminosity, Seismic analysis, 0103 physical sciences, Astrophysics::Solar and Stellar Astrophysics, 010303 astronomy & astrophysics, Stellar evolution, Solar and Stellar Astrophysics (astro-ph.SR), Astrophysics::Galaxy Astrophysics, Physics, [PHYS]Physics [physics], 010308 nuclear & particles physics, Mode (statistics), Astronomy and Astrophysics, stars: solar-type, Radius, Effective temperature, Stars, Astrophysics - Solar and Stellar Astrophysics, 13. Climate action, Space and Planetary Science, Astrophysics::Earth and Planetary Astrophysics, stars: fundamental parameters
Abstract: Solar analogues are important stars to study for understanding the properties of the Sun. Evolutionary modeling, combined with seismic and spectroscopic analysis, becomes a powerful method to characterize stellar intrinsic parameters, such as mass, radius, metallicity and age.However, these characteristics, relevant for other aspects of astrophysics or exoplanetary system physics for example, are difficult to obtain with a high precision and/or accuracy. The goal of this study is to characterize the two solar analogues HD42618 and HD43587, observed by CoRoT. In particular, we aim to infer precise mass, radius, and age, using evolutionary modeling constrained by spectroscopic, photometric, and seismic analysis. These stars show evidences of being older than the Sun but with a relatively large lithium abundance. We present the seismic analysis of HD42618, and the modeling of the two solar analogs HD42618 andHD43587 using the CESTAM stellar evolution code. Models were computed to reproduce the spectroscopic (effective temperature and metallicity) and seismic (mode frequencies) data,and the luminosity of the stars, based on Gaia parallaxes. We infer very similar values of mass and radius for both stars compared to the literature, within the uncertainties, and reproduce correctly the seismic constraints. For HD42618, the modeling shows it is slightly less massive and older than the Sun. For HD43587, it confirms it is more massive and older than the Sun,in agreement with previous results. The use of chemical clocks improves the reliability of our age estimates., 8 pages, 5 figures, 4 tables
Published: 2021
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28. Potential of the predatory mite Amblydromalus zannoui to control pest mites on Jatropha curcas

Author: Emiliano Brandão de Azevedo, Wilton Pires da Cruz, Raphael C. Castilho, Sofía Jiménez Jorge, Gilberto J. de Moraes, Marie-Stéphane Tixier, Valdirene Coutinho Miranda, Marçal Pedro-Neto, Renato de Almeida Sarmento, Universidade Federal do Tocantins (UFT), Universidade Federal do Tocantins, Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Univ Sao Paulo, ESALQ, BR-13418900 Sao Paulo, Brazil, Partenaires INRAE, This study was financed in part by the CoordenacAo de Aperfeicoamento de Pessoal de Nivel Superior - Brazil (CAPES-PROCAD-AMAZONIA). We thank the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq - Project: 306652/2018-8) for financially supporting this research., Federal University of Tocantins (UFT), Universidade Estadual Paulista (Unesp), Univ. Montpellier, and Universidade de São Paulo (USP)
Subjects: 0106 biological sciences, [SDV]Life Sciences [q-bio], Population, Polyphagotarsonemus latus, Tarsonemidae, 010603 evolutionary biology, 01 natural sciences, Mite, Castor bean pollen, education, PÓLEN, education.field_of_study, biology, Prostigmata, 15. Life on land, biology.organism_classification, 010602 entomology, Horticulture, Animal ecology, Insect Science, Biological control, Tetranychus, Tetranychidae, Agronomy and Crop Science, Jatropha curcas, Predator
Abstract: Made available in DSpace on 2021-06-25T10:23:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Amblydromalus zannoui Sourassou, Sarmento and Moraes is a phytoseiid mite of the limonicus group described from central Brazil from leaves of physic nut, Jatropha curcas L. (Malpighiales: Euphorbiaceae), a plant potentially useful as a source of biofuel. This plant is often attacked by the mites Polyphagotarsonemus latus (Banks) (Prostigmata: Tarsonemidae) and Tetranychus bastosi Tuttle, Baker and Sales (Prostigmata: Tetranychidae). The objectives of this work were to evaluate the predation rate of A. zannoui on those phytophagous mites, to assess its life cycle on these prey and on pollen of Ricinus communis L. (Euphorbiaceae), and to determine its attraction to physic nut leaves infested by P. latus or T. bastosi. Amblydromalus zannoui attacked more nymphs and adults than eggs of P. latus, and more eggs and larvae than adults of T. bastosi. The life table parameters suggest that A. zannoui performs better on P. latus (rm: 0.20, Ro: 18.77; λ: 1.23) and pollen (rm: 0.18, Ro: 23.32, λ: 1.18). The predator seems to be attracted to plants with P. latus, but attraction was not clear cut for plants with T. bastosi. The results suggested that A. zannoui is a potential control agent to be used against P. latus on physic nut plants, and that R. communis pollen can be used as supplementary food to maintain the predator population in the absence of prey. Federal University of Tocantins (UFT), PO BOX 66 Universidade Estadual Paulista (UNESP) CBGP Montpellier SupAgro INRA CIRAD IRD Univ. Montpellier Campus International de Baillarguet, CS 30016 Departamento de Entomologia Fitopatologia E Zoologia Agrícola ESALQ-USP Universidade Estadual Paulista (UNESP)
Published: 2021
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29. Dual-process of starch modification: Combining ozone and dry heating treatments to modify cassava starch structure and functionality

Author: Alain Le-Bail, Bianca Chieregato Maniglia, Manoel Divino da Matta Junior, Pedro Esteves Duarte Augusto, Dâmaris Carvalho Lima, Patricia Le-Bail, ESALQ ( ~ 'Luiz de Queiroz' College of Agriculture), LQCE (Laboratory of Chemistry, Cellulose and Energy), University of São Paulo, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ONIRIS GEPEA NANTES, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), University of São Paulo (USP), Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) : 2019/05043-6, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ): 306557/2017-7, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior -Brasil (CAPES, Brazil) : 001, and Region Pays de la Loire (France) through the RFI 'FOOD 4 TOMORROW' program.
Subjects: endocrine system, Manihot, Ozone, Chemical Phenomena, Starch, 02 engineering and technology, Starch modification, Biochemistry, Modified starch, Heating, Structure-Activity Relationship, 03 medical and health sciences, chemistry.chemical_compound, X-Ray Diffraction, Molecular size, PROCESSAMENTO DE ALIMENTOS, Structural Biology, Dry heating, DHT, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Molecular Biology, Mechanical Phenomena, 030304 developmental biology, 0303 health sciences, Molecular Structure, Chemical modification, food and beverages, Hydrogels, General Medicine, Hydrogen-Ion Concentration, 021001 nanoscience & nanotechnology, Amorphous solid, Molecular Weight, chemistry, Chemical engineering, Scientific method, 0210 nano-technology, hormones, hormone substitutes, and hormone antagonists
Abstract: International audience; This work evaluated for the first time the effect of dual modification of cassava starch by using ozone (O-3) and dry heating treatment (DHT). The dual modification was capable to promote fissures on the surface of the starch granule (DHT + O-3), affected the starch amorphous domains, presented greater degree of starch oxidation (DHT + O-3) and different profiles of starch molecular size distribution. These modifications resulted in starches with different properties. Moreover, the sequence of treatments was decisive for the hydrogel properties: while DHT + O-3 resulted in formation of stronger gels, O-3 + DHT resulted in weaker gels. In conclusion, this proposed dual modification was capable to produce specific modified starch when compared with the isolated treatments, also expanding the potential of cassava starch applications. (C) 2020 Elsevier B.V. All rights reserved.
Published: 2021
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30. Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

Author: Brenda N Porto, Ana Cristina Miranda Brasileiro, A. L. Lacerda, Thais Nicolini Oliveira, Etienne Danchin, C. C. Vinson, Iracyara Sampaio, Thomas C. R. Williams, Patricia Messenberg Guimaraes, Ana Paula Zotta Mota, Brazilian Agricultural Research Corporation (Embrapa), Universidade de Brasilia [Brasília] (UnB), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), INCT PlantStress Biotech 465480/2014-4, FAPDF (Distrito Federal Research Foundation) 0193.001565/2017, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), Science without Boarders CNPq program, CHRISTINA C. VINSON, UNB, ANA P. Z. MOTA, BRENDA N. PORTO, THAIS N. OLIVEIRA, IRACYARA SAMPAIO, UNB, ANA L. LACERDA, ETIENNE G. J. DANCHIN, UNIVERSITÉ CÔTE D'AZUR, FRANCE, PATRICIA MESSEMBERG GUIMARAES, Cenargen, THOMAS C. R. WILLIAMS, UNB, and ANA CRISTINA MIRANDA BRASILEIRO, Cenargen.
Subjects: 0106 biological sciences, 0301 basic medicine, Arachis, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV]Life Sciences [q-bio], Arabidopsis, lcsh:Medicine, Oligosaccharides, Disaccharides, 01 natural sciences, Article, Arachis duranensis, 03 medical and health sciences, chemistry.chemical_compound, Raffinose, Gene Expression Regulation, Plant, Stress, Physiological, lcsh:Science, Plant Proteins, 2. Zero hunger, Genetics, Abiotic component, [SDV.GEN]Life Sciences [q-bio]/Genetics, Multidisciplinary, beta-Fructofuranosidase, biology, Abiotic stress, Transgenic plants, lcsh:R, Fabaceae, 15. Life on land, Galactosyltransferases, Plants, Genetically Modified, biology.organism_classification, Gene expression profiling, Droughts, 030104 developmental biology, chemistry, RFOs, lcsh:Q, 010606 plant biology & botany
Abstract: Raffinose family oligosaccharides (RFOs) are implicated in plant regulatory mechanisms of abiotic stresses tolerance and, despite their antinutritional proprieties in grain legumes, little information is available about the enzymes involved in RFO metabolism in Fabaceae species. In the present study, the systematic survey of legume proteins belonging to five key enzymes involved in the metabolism of RFOs (galactinol synthase, raffinose synthase, stachyose synthase, alpha-galactosidase, and beta-fructofuranosidase) identified 28 coding-genes in Arachis duranensis and 31 in A. ipaënsis. Their phylogenetic relationships, gene structures, protein domains, and chromosome distribution patterns were also determined. Based on the expression profiling of these genes under water deficit treatments, a galactinol synthase candidate gene (AdGolS3) was identified in A. duranensis. Transgenic Arabidopsis plants overexpressing AdGolS3 exhibited increased levels of raffinose and reduced stress symptoms under drought, osmotic, and salt stresses. Metabolite and expression profiling suggested that AdGolS3 overexpression was associated with fewer metabolic perturbations under drought stress, together with better protection against oxidative damage. Overall, this study enabled the identification of a promising GolS candidate gene for metabolic engineering of sugars to improve abiotic stress tolerance in crops, whilst also contributing to the understanding of RFO metabolism in legume species.
Published: 2020
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31. Detrimental sublethal effects hamper the effective use of natural and chemical pesticides in combination with a key natural enemy of Bemisia tabaci on tomato

Author: Marcelo Mendes Haro, Marianne A. Soares, Antonio Biondi, Luis C. Passos, Geraldo Andrade Carvalho, Lucia Zappalà, Nicolas Desneux, Anne-Violette Lavoir, Mateus R. Campos, Federal University of Lavras, Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), University of Catania [Italy], Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (EPAGRI), Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) 88881.132785/2016-2101, Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), IPM Innovation Lab AID-OAA-14-000018, European Project: 633999,H2020,H2020-SFS-2014-2,EUCLID(2015), European Project: 618127,EC:FP7:KBBE,FP7-ERANET-2013-RTD,ARIMNET2(2014), Universidade Federal de Lavras = Federal University of Lavras (UFLA), and Université Nice Sophia Antipolis (1965 - 2019) (UNS)
Subjects: 0106 biological sciences, Integrated pest management, Insecticides, [SDV]Life Sciences [q-bio], Biological pest control, biological control, Whitefly, 01 natural sciences, Predation, ecotoxicology, Toxicology, Hemiptera, chemistry.chemical_compound, Solanum lycopersicum, whitefly, Animals, 2. Zero hunger, Pyrethroid, biology, integrated pest management, predatory mirids, General Medicine, Pesticide, biology.organism_classification, Miridae, 010602 entomology, chemistry, Insect Science, Predatory Behavior, [SDE]Environmental Sciences, PEST analysis, Agronomy and Crop Science, 010606 plant biology & botany
Abstract: International audience; Background Bemisia tabaci(Hemiptera: Aleyrodidae) represents one of the greatest threats to agricultural crops. Chemical control is the primary tool used in integrated pest management (IPM) programs. However, release of the predatorNesidiocoris tenuis(Hemiptera: Miridae) on tomato plants is a highly recommended control tactic. The objective of this study was to evaluate the efficacy of a commercial borax plus citrus oil (BCO) product againstB. tabaciin the presence and absence ofN. tenuis. The synthetic insecticide lambda-cyhalothrin was used as a positive control. We also evaluated the sublethal effects of BCO on the behavior and predation rate ofN. tenuis. Results Our results demonstrated that BCO, alone and at its maximum recommended field rate forB. tabaci, was not effective in controlling the pest under laboratory conditions. Application of BCO simultaneous withN. tenuisrelease did not reduce the increase in theB. tabacipopulation. Effective control ofB. tabaciwas achieved using onlyN. tenuis. However, synthetic lambda-cyhalothrin pyrethroid, used here as a control, caused high pest mortality and led to on-site extinction ofN. tenuis, which did not occur for insects exposed to BCO. Lambda-cyhalothrin and BCO significantly affected the foraging behavior ofN. tenuis, reducing the predation rate, especially following exposure to lambda-cyhalothrin. Conclusion The insecticide lambda-cyhalothrin achieved satisfactory results in suppressingB. tabaci, but was harmful toN. tenuis. Additionally, lambda-cyhalothrin and BCO affected predator behavior.
Published: 2020
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32. Effects of maternal low-protein diet and spontaneous physical activity on the transcription of neurotrophic factors in the placenta and the brains of mothers and offspring rats

Author: Helyson Thomaz da Silva, Jéssica Fragoso, Rubem Carlos Araújo Guedes, Viviane de Oliveira Nogueira Souza, João Henrique Costa-Silva, Emmanuelle Loizon, Carol Góis Leandro, Luciano Pirola, Raquel da Silva Aragão, Hubert Vidal, Gabriela Carvalho Jurema Santos, Federal University of Pernambuco [Recife], Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)302515/2015-1Coordination for the Improvement of Higher Level (or Education) Personnel (CAPES-COFECUB)Me797-14Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)APQ: 0797-4.05/14APQ-0201-4.05/14, and CarMeN, laboratoire
Subjects: 0301 basic medicine, Male, medicine.medical_specialty, Offspring, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Placenta, neuroplasticity, Medicine (miscellaneous), Tropomyosin receptor kinase B, Biology, 03 medical and health sciences, 0302 clinical medicine, Low-protein diet, Neurotrophic factors, Pregnancy, Internal medicine, Physical Conditioning, Animal, medicine, Diet, Protein-Restricted, Animals, protein restriction, Nerve Growth Factors, Rats, Wistar, 2. Zero hunger, Fetus, Neuronal Plasticity, Physical activity, Brain, Maternal Nutritional Physiological Phenomena, Placentation, [SDV] Life Sciences [q-bio], rats, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Hypothalamus, developmental plasticity, Gestation, Female, 030217 neurology & neurosurgery
Abstract: Maternal protein restriction and physical activity can affect the interaction mother–placenta–fetus. This study quantified the gene expression of brain-derived neurotrophic factor (BDNF), neurothrophin 4, tyrosine kinase receptor B (TrkB/NTRK2), insulin-like growth factor (IGF-1), and insulin-like growth factor receptor (IGF-1r) in the different areas of mother’s brain (hypothalamus, hippocampus, and cortex), placenta, and fetus’ brain of rats. Female Wistar rats (n = 20) were housed in cages containing a running wheel for 4 weeks before gestation. According to the distance spontaneously traveled daily, rats were classified as inactive or active. During gestation, on continued access to the running wheel, active and inactive groups were randomized to receive normoprotein diet (18% protein) or a low-protein (LP) diet (8% protein). At day 20 of gestation, gene expression of neurotrophic factors was analyzed by quantitative polymerase chain reaction in different brain areas and the placenta. Dams submitted to a LP diet during gestation showed upregulation of IGF-1r and BDNF messenger RNA in the hypothalamus, IGF-1r and NTRK2 in the hippocampus, and BDNF, NTRK2, IGF-1 and IGF-1r in the cortex. In the placenta, there was a downregulation of IGF-1. In the brain of pups from mothers on LP diet, IGF-1r and NTRK2 were downregulated. Voluntary physical activity attenuated the effects of LP diet on IGF-1r in the hypothalamus, IGF-1r and NTRK2 in the hippocampus, IGF-1 in the placenta, and NTRK2 in the fetus’ brain. In conclusion, both maternal protein restriction and spontaneous physical activity influence the gene expression of BDNF, NTRK2, IGF-1, and IGF-1r, with spontaneous physical activity being able to normalize in part the defects caused by protein restriction during pregnancy.
Published: 2020
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33. Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis

Author: Sylvie Nonin-Lecomte, Reynald Gillet, Muriel Primon-Barros, Rafael Gomes Von Borowski, Sophie Chat, Alexandre José Macedo, Rafael de Oliveira Schneider, Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Cibles Thérapeutiques et conception de médicaments (CiTCoM - UMR 8038), Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Brazilian Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)CAPES [001], Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)National Council for Scientific and Technological Development (CNPq), Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS-PRONEN), French Centre National de Recherche Scientifique (CNRS)Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)
Subjects: Microbiology (medical), Pseudonajide, Hepcidinas, Venenos de serpentes, Cell Survival, Amino Acid Motifs, Antimicrobial peptides, lcsh:QR1-502, Gene Expression, Peptide, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Biology, Microbiology, Permeability, Bacterial cell structure, lcsh:Microbiology, biofilm, Cell Line, 03 medical and health sciences, Cell Wall, Staphylococcus epidermidis, Animals, Humans, 030304 developmental biology, snake venom, chemistry.chemical_classification, 0303 health sciences, 030306 microbiology, Cell Membrane, Biofilm, Antimicrobial, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Anti-Bacterial Agents, 3. Good health, Teichoic Acids, chemistry, Snake venom, pseudonajide, Biofilms, Cell envelope, Antimicrobial peptide, Biofilmes, Antimicrobial Cationic Peptides, Snake Venoms, Research Article
Abstract: Background The increase in bacterial resistance phenotype cases is a global health problem. New strategies must be explored by the scientific community in order to create new treatment alternatives. Animal venoms are a good source for antimicrobial peptides (AMPs), which are excellent candidates for new antimicrobial drug development. Cathelicidin-related antimicrobial peptides (CRAMPs) from snake venoms have been studied as a model for the design of new antimicrobial pharmaceuticals against bacterial infections. Results In this study we present an 11 amino acid-long peptide, named pseudonajide, which is derived from a Pseudonaja textilis venom peptide and has antimicrobial and antibiofilm activity against Staphylococcus epidermidis. Pseudonajide was selected based on the sequence alignments of various snake venom peptides that displayed activity against bacteria. Antibiofilm activity assays with pseudonajide concentrations ranging from 3.12 to 100 μM showed that the lowest concentration to inhibit biofilm formation was 25 μM. Microscopy analysis demonstrated that pseudonajide interacts with the bacterial cell envelope, disrupting the cell walls and membranes, leading to morphological defects in prokaryotes. Conclusions Our results suggest that pseudonajide’s positives charges interact with negatively charged cell wall components of S. epidermidis, leading to cell damage and inhibiting biofilm formation.
Published: 2020
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34. Dry heating treatment: A potential tool to improve the wheat starch properties for 3D food printing application

Author: Pedro Esteves Duarte Augusto, Patricia Le-Bail, Dâmaris Carvalho Lima, Anthony Ogé, Manoel Divino Da Matta Junior, Bianca Chieregato Maniglia, Alain Le-Bail, Ingénierie des Biopolymères pour la Structuration de Matrices et des Matériaux (SFR IBSM), Université de Nantes (UN)-Institut Mines-Télécom [Paris] (IMT)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UNIVERSIDADE DE SAO PAULO PIRACICABA BRA, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), University of São Paulo (USP), Region Pays de la Loire (France)/RFI 'FOOD 4 TOMORROW', Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) : 2019/05043-6, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) : 306557/2017-7, and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) : 001.
Subjects: Additive manufacturing, Starch, 02 engineering and technology, Modified starch, Heating, Food texture, chemistry.chemical_compound, Crystallinity, 0404 agricultural biotechnology, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Personalized food, Triticum, 2. Zero hunger, Syneresis, Depolymerization, Granule (cell biology), food and beverages, Reproducibility of Results, 04 agricultural and veterinary sciences, [PHYS.MECA.MSMECA]Physics [physics]/Mechanics [physics]/Materials and structures in mechanics [physics.class-ph], Apparent viscosity, 021001 nanoscience & nanotechnology, 040401 food science, chemistry, Chemical engineering, 3D food printing, Self-healing hydrogels, Printing, Three-Dimensional, 0210 nano-technology, TRIGO, Food Science
Abstract: International audience; The futuristic technology of three-dimensional (3D) printing is an additive manufacturing that allows obtaining creative and personalized food products. In this context, the study of food formulations (named as "inks") to be processed through 3D printing is necessary. This work investigated the use of dry heating treatment (DHT), a simple and safe method, to improve the wheat starch properties aiming to produce hydrogels to be used as "inks" for 3D printing. Wheat starch was processed by dry heating for 2 (DHT_2h) and 4 h (DHT_4h) at 130 degrees C. Modified wheat starches showed an increase in granule size, but processing did not alter the granule's shape nor surface, neither alter the molecular functional groups. On the other hand, DHT promoted slight molecular depolymerization, and reduction of starch crystallinity. Hydrogels "inks" based on the modified starches showed lower peak apparent viscosity during pasting, higher structural strength at rest, higher resistance to external stresses, higher gel firmness, and lower syneresis than hydrogels based on native starch. The hydrogels based on starch DHT_4h showed the best printability (greater ability to make a 3D-object by layer-by-layer deposition and to support its structure once printed) and this "ink" showed better reproducibility. Another point observed is that DHT extended the texture possibilities of printed samples based on wheat starch hydrogels. These results suggested that DHT is a relevant process to improve the properties of hydrogels based on wheat starch, making this ink suitable for 3D printing application.
Published: 2020
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35. Ultraspecific live imaging of the dynamics of zebrafish neutrophil granules by a histopermeable fluorogenic benzochalcone probe

Author: Philippe Herbomel, Benoit S. Marteyn, Romain Duval, Suellen D. S. Oliveira, Emma Colucci-Guyon, Ismael C. Bellettini, Magali Blaud, Ariane S. Batista, Karine Leblanc, Macrophages et Développement de l’Immunité, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Universidade Federal do Rio de Janeiro [Rio de Janeiro] (UFRJ), University of Illinois [Chicago] (UIC), University of Illinois System, Laboratoire de cristallographie et RMN biologiques (LCRB - UMR 8015), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Universidade Federal de Santa Catarina [Florianópolis] (UFSC), Biologie et Biotechnologie des Cyanobactéries (B2CYA), Département Microbiologie (Dpt Microbio), Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), BioCIS, Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD), Grants from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPq (401897/2010-9 to R. D.), the Institut Pasteur (GPF Microbes and Brain Myconeuro, S-PI14032-02G to E. C.-G.) and the Fondation pour la Recherche Médicale (Equipe FRM DEQ20120323714 to P. H.) are acknowledged, as well as Dr J. Moreira for translating the CNPq scientific proposal into Portuguese., Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Universidade Federal do Rio de Janeiro (UFRJ), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Universidade Federal de Santa Catarina = Federal University of Santa Catarina [Florianópolis] (UFSC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), and ANR-17-CE15-0012,NEUTROXIA,Effet de l'exposition des neutrophiles à l'oxygène sur leur activation et leur mort : une lame à double tranchant(2017)
Subjects: Cell type, animal structures, [SDV]Life Sciences [q-bio], Phenotypic screening, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, 010402 general chemistry, 01 natural sciences, Live cell imaging, Organelle, [CHIM]Chemical Sciences, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Zebrafish, Innate immune system, biology, [CHIM.ORGA]Chemical Sciences/Organic chemistry, 010405 organic chemistry, Chemistry, fungi, General Chemistry, biology.organism_classification, 0104 chemical sciences, Cell biology, Staining, Vital stain, embryonic structures, [SDV.IMM]Life Sciences [q-bio]/Immunology
Abstract: A fluorogenic benzochalcone specifically labels live neutrophil granules in whole wild-type, GFP- or RFP-expressing zebrafish embryos and larvae., Neutrophil granules (NGs) are key components of the innate immune response and mark the development of neutrophilic granulocytes in mammals. However, there has been no specific fluorescent vital stain up to now to monitor their dynamics within a whole live organism. We rationally designed a benzochalcone fluorescent probe (HAB) featuring high tissue permeability and optimal photophysics such as elevated quantum yield, pronounced solvatochromism and target-induced fluorogenesis. Phenotypic screening identified HAB as the first cell- and organelle-specific small-molecule fluorescent tracer of NGs in live zebrafish larvae, with no labeling of other cell types or organelles. HAB staining was independent of the state of neutrophil activation, labeling NGs of both resting and phagocytically active neutrophils with equal specificity. By high-resolution live imaging, we documented the dynamics of HAB-stained NGs during phagocytosis. Upon zymosan injection, labeled NGs were rapidly recruited to the forming phagosomes. Despite being a reversible ligand, HAB could not be displaced by high concentrations of pharmacologically relevant competing chalcones, indicating that this specific labeling was the result of the HAB's precise physicochemical signature rather than a general feature of chalcones. However, one of the competitors was discovered as a promising interstitial fluorescent tracer illuminating zebrafish histology, similarly to BODIPY-ceramide. As a yellow-emitting histopermeable vital stain, HAB functionally and spectrally complements most genetically incorporated fluorescent tags commonly used in live zebrafish biology, holding promise for the study of neutrophil-dependent responses relevant to human physiopathology such as developmental defects, inflammation and infection. Furthermore, HAB intensely labeled isolated live human neutrophils at the level of granulated subcellular structures consistent with human NGs, suggesting that the labeling of NGs by HAB is not restricted to the zebrafish model but also relevant to mammalian systems.
Published: 2019
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36. In vitro models for investigation of the host-parasite interface - possible applications in acute Chagas disease

Author: Juliana Lott Carvalho, Mariana Hecht, Nadjar Nitz, Ester Rose, Natalia Martins Breyner, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Brasilia [Brazil] (UnB), Universidade Católica de Brasília (UCB), and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) Finance Code 001, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq: 421897/2016-3), and Fundacao de Apoio a Pesquisa do Distrito Federal) (FAPDF): 193.001.471/2017.
Subjects: 0301 basic medicine, Chagas disease, Trypanosoma cruzi, Veterinary (miscellaneous), [SDV]Life Sciences [q-bio], 030231 tropical medicine, Translational research, Disease, Host-Parasite Interactions, 03 medical and health sciences, 0302 clinical medicine, Pluripotent stem cells, In vivo, medicine, Animals, Humans, Parasite hosting, Cells, Cultured, biology, In vitro models, 030108 mycology & parasitology, medicine.disease, biology.organism_classification, In vitro, 3. Good health, Organoids, Infectious Diseases, Insect Science, Parasitic disease, Immunology, Parasitology, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Chagas disease (CD), caused by Trypanosoma cruzi, is the main parasitic disease in the Western Hemisphere, with an increasing number of cases, especially in non-endemic regions. The disease is characterized by cardiomegaly and mega viscera, nevertheless, the clinical outcome is hard to predict, underscoring the need for further research into the pathophysiology of CD. Even though most basic and translational research involving CD is performed using in vivo models, in vitro models arise as an ethical, rapidly evolving, and physiologically relevant alternative for CD research. In the present review, we discuss the past and recent in vitro models available to study the host-parasite interface in cardiac and intestinal CD, critically analyzing the possibilities and limitations of state-of-the-art alternatives for the CD host-parasite investigation.
Published: 2020
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37. Phylogeny and evolution of the genus Ctenocolum Kingsolver & Whitehead (Coleoptera, Chrysomelidae, Bruchinae), with the description of three new species

Author: Cibele S. Ribeiro-Costa, Gael J. Kergoat, Daiara Manfio, Isaac Reis Jorge, Universidade Tecnológica Federal do Paraná [Curitiba] (UTFPR), Laboratório de Sistemática e Bioecologia de Coleoptera, Departamento de Zoologia, Universidade Federal do Paraná (UFPR), Universidade Federal do Parana [Curitiba] (UFPR), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), We thank Alexander Konstantinov and Elisabeth Roberts, United States National Museum, Smithsonian Institution, that kindly received the two first authors in the USNM and for the loan of specimens that enable this study, and the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for the scholarship for the first author and the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for the scholarships for the second and fourth authors. This is the contribution number 1937 of the Departamento de Zoologia, Universidade Federal do Parana, Curitiba, Brazil.
Subjects: 0106 biological sciences, Biogeography, 010607 zoology, Zoology, Biology, 010603 evolutionary biology, 01 natural sciences, taxonomy, Monophyly, Genus, Phylogenetics, bruchinae, phylogenetic analyses, Western hemisphere, biogeography, Ecology, Evolution, Behavior and Systematics, new species, Synapomorphy, Ecology, Phylogenetic tree, [SDV.BA]Life Sciences [q-bio]/Animal biology, 15. Life on land, acanthoscelidina, host plants, Insect Science, Type locality, Taxonomy (biology)
Abstract: The seed beetle genus Ctenocolum Kingsolver & Whitehead is peculiar because its preferred host Lonchocharpus Kunth (Fabaceae) is not preyed upon by other bruchine species. This study investigates the phylogenetic relationships and evolution of this genus and of its species groups, while providing the description of three new species and of the male of C. biolleyi Kingsolver & Whitehead. To infer phylogenetic relationships, a character matrix of 40 morphological characters was assembled and analysed using both parsimony and Bayesian inference. Ancestral state estimations of host plant use and biogeography analyses were also performed. A total of 22 species were examined: 16 Ctenocolum species (including the three new ones) and six outgroup bruchine species (from genera Caryedes Hummel, Meibomeus Bridwell, Pygiopachymerus Pic and Pachymerus Thunberg). All resulting trees support the monophyly of the genus Ctenocolum. Three synapomorphies characterize the genus: (i) head with frontal carina enlarged at base, (ii) male pygidium truncated apically, and (iii) lateral lobes of tegmen with dorsal process. The two known species groups are also recovered monophyletic in the parsimony analyses. The following three species are described: Ctenocolum inmaculatus Manfio & Ribeiro-Costa sp. nov. (Type locality: Venezuela, Guarico), which belongs to the group tuberculatum; Ctenocolum nigronotus Manfio & Ribeiro-Costa sp. nov. (Type locality: Porto Rico, Mayaguez) and C. pallidus Manfio & Ribeiro-Costa sp. nov. (Type locality: Republic of Guyana), which belong to the group podagricus. Finally, we present colored illustrations of dorsal patterns and male genitalia for these three new species and C. biolleyi in addition to an updated key for the genus Ctenocolum.
Published: 2018
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38. Red disperse dyes (DR 60, DR 73 and DR 78) at environmentally realistic concentrations impact biochemical profile of early life stages of zebrafish (Danio rerio)

Author: Danielle Palma de Oliveira, Carlos Gravato, Gabriela Meireles, Ana Letícia Madeira Sanches, Amadeu M.V.M. Soares, Maria Valnice Boldrin Zanoni, Michiel A. Daam, Universidade de São Paulo (USP), University of Aveiro, New University of Lisbon, Universidade Estadual Paulista (Unesp), University of Lisboa, and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Subjects: 0301 basic medicine, Cellular energy allocation, Embryo, Nonmammalian, Antioxidant, medicine.medical_treatment, 010501 environmental sciences, Toxicology, medicine.disease_cause, 01 natural sciences, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, Neurotoxicity, medicine, Animals, Textile dyes, Coloring Agents, Zebrafish, 0105 earth and related environmental sciences, Dose-Response Relationship, Drug, Molecular Structure, biology, General Medicine, Glutathione, biology.organism_classification, Acetylcholine, 030104 developmental biology, chemistry, Biochemistry, Oxidative stress, Catalase, Environmental toxicology, Toxicity, biology.protein, Azo Compounds, Water Pollutants, Chemical
Abstract: Made available in DSpace on 2018-12-11T16:54:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-08-25 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Federación Española de Enfermedades Raras Fundació Catalana de Trasplantament Centro de Estudos Ambientais e Marinhos, Universidade de Aveiro Dyes have been used for more than twenty thousand years in textile, pharmaceutical, food, cosmetic, and photographic industries, among others. Despite their importance in these applications, dyes can be toxic and resistant to many degradation processes used in wastewater treatment plants. Although a large number of dyes have been released in the environments in high amounts, studies into the environmental toxicity of these substances are still scarce. The aim of this study was to evaluate the potential toxic effects of textile dyes Disperse Red 60, Disperse Red 73 and Disperse Red 78 in zebrafish early life stages. To this end, biochemical biomarkers were selected to evaluate non-enzimatic antioxidant (Total Glutathione), antioxidant enzymes (Glutathione S-transferase and Catalase), oxidative stress (lipid peroxidation), neurotransmission (acetylcholinesterase) and energetic metabolism parameters (energy available and energy consumed) after 96 h exposure to these dyes. Our results demonstrated that these disperse dyes induce biochemical alterations in zebrafish embryos at environmental realistic concentrations and that the discharge of these disperse dyes into water bodies should be carefully evaluated. The selected biomarkers were sensitive as early-warning endpoints of disperse dyes toxicity on zebrafish embryos. Implications for risk assessment and indications for future research are discussed. School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo Department of Biology and CESAM University of Aveiro CENSE Department of Environmental Sciences and Engineering Faculty of Sciences and Technology New University of Lisbon, Quinta da Torre NEEA/CRHEA/SHS São Carlos Engineering School University of São Paulo Institute of Chemistry-State University “Julio de Mesquita Filho”-UNESP Faculty of Sciences and CESAM University of Lisboa, Campo Alegre National Institute of Science and Technology for Detection Toxicological Evaluation and Removal of Emerging and Radioactive Contaminants (INCT-DATREM) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Institute of Chemistry-State University “Julio de Mesquita Filho”-UNESP CNPq: 142379/2014-0 FAPESP: 2014/14621-0 FAPESP: 2016/03281-9 FAPESP: 2017/05543-3 CAPES: 88881.134597/2016-01 Federación Española de Enfermedades Raras: PT2020 Fundació Catalana de Trasplantament: SFRH/BPD/109199/2015 Centro de Estudos Ambientais e Marinhos, Universidade de Aveiro: UID/AMB/50017
Published: 2018
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39. Syntheses of non-aromatic medium and large rings synthesized via phenylnitrenium ions

Author: Lilian Y. Watanabe, Paulo Marcos Donate, Joaquín M. Campos, Fernando Costa Archanjo, Gino Del Ponte, [Del Ponte, Gino] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Via Cafe S-N, BR-14040903 Ribeirao Preto, SP, Brazil, [Archanjo, Fernando C.] Univ Fed Vales Jequitinhonha & Mucuri, Fac Ciencias Biol & Saude, Dept Farm, Campus JK,Rodovia MGT 367,Km 583, BR-39100000 Diamantina, MG, Brazil, [Watanabe, Lilian Y.] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP, Brazil, [Donate, Paulo M.] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP, Brazil, [Campos, Joaquin M.] Fac Farm, Dept Quim Farmaceut & Organ, C Campus Cartuja S-N, Granada 18071, Spain, [Campos, Joaquin M.] Univ Granada, SAS, Inst Biosanitario Granada Ibs GRANADA, Granada, Spain, Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), and Coordenadoria de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Subjects: ÍONS, Medium rings, Arylnitrenium ions, Chemistry(all), Stereochemistry, General Chemical Engineering, Reactivities, Nitroarenes, 010402 general chemistry, 01 natural sciences, Medicinal chemistry, Alkylnitrenium ions, lcsh:Chemistry, chemistry.chemical_compound, Laser flash-photolysis, Singlet-triplet gaps, Nitrenium ions, Remote intramolecular functionalization, Trifluoroacetic acid, Anilines, Carboxylate, Aryl azides, Chloroform, 010405 organic chemistry, Regioselectivity, Precursors, General Chemistry, Butene, 0104 chemical sciences, chemistry, lcsh:QD1-999, Cyclization, Yield (chemistry), Phenylnitrenium, Chemical Engineering(all), Substitution, Cyclooctadiene, Methyl group, Large rings
Abstract: We describe the preparation of m-and p-substituted phenyl azides which, on treatment with trifluoromethanesulfonic acid in chloroform (and only in one case, after adding trifluoroacetic acid) at 0 degrees C, gives rise to the intermediate phenylnitrenium ions that undergo intramolecular cyclization to give six-, eight-membered carbocycles, and ten-membered heterocycles. Intramolecular cyclization of 1-(4-azidophenyl)-4-phenylbutane (3b) gives direct access to the 1,2,3,4-tetrahydronaphthalene lignan scaffold with a good yield. When the same reaction is carried out on 1-(3-azidophenyl)-4-phenylbutane (3a), the meta isomer of 3b, the 3-aminodibenzo[a, c] cyclooctadiene is obtained with a modest yield. When an ethoxycarbonyl group is introduced at position two of the butene chain [16a, as an E/Z mixture (1/4)], the ethyl 3-aminobenzo[a, c] octatriene carboxylate was the major compound, and the 10-membered heterocycle the minor one, both derived from (E)-16a. Finally, when a methyl group is located at the para position of the azido group [(E)1-6b], cyclization involves the carbon atom ortho to the nitrogen atom and the ethyl 4-methyl-1-tosylaminobenzo[ a, c] octatriene carboxylate is the only compound obtained, after treatment with tosyl chloride. With all these structural changes, we have switched over from the formation of mixtures of compounds to the regioselective formation of the target molecule, suggesting the corresponding mechanism of reaction and expanding the knowledge of this type of reaction. (C) 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
Published: 2018

40. A global database for metacommunity ecology, integrating species, traits, environment and space

Author: Ramiro de Campos, Julia Ramos Miranda, Sandrine Pavoine, Sylvain Dolédec, Joshua Adam Drew, David Mouillot, Elizabeth C. Lowe, Robin J. Pakeman, Willem Renema, Heloise Gibb, Nicole J. de Voogd, Sen-Her Shieh, Triin Reitalu, Stéphane Chantepie, Jan Żarnowiec, Guillaume Fried, Roel van Klink, Núria Bonada, Sébastien Villéger, Daniel F. R. Cleary, Anthony Eallonardo, Damian Chmura, Bjorn J. M. Robroek, William K. Cornwell, Monika Staniaszek-Kik, Christoph F. J. Meyer, Martin J. Westgate, Valérie Raevel, Cédric Frenette-Dussault, Frank Dziock, Cajo J. F. ter Braak, Alienor Jeliazkov, Nadia Barsoum, Adrià López-Baucells, Philippe Choler, Darko Mijatovic, Anik Brind'Amour, Michal Stanko, Helena Castro, Mark C. Urban, Belinda Gallardo, Maxim Zolotarev, Francesco Pomati, Alessandro Ossola, Andrés Mellado-Díaz, Luc Barbaro, Raphaël Arlettaz, R.M.A. Wegman, Thiago Gonçalves-Souza, Melanie J. Edgar, Alena Bartonova, Francisco Leonardo Tejerina-Garro, Nigel R. Andrew, Burak K. Pekin, Myles H. M. Menz, Karen M. Chong-Seng, Joan Pino, Jonathan M. Chase, Elena Belskaya, Jean-Yves Humbert, Ignacio Ribera, Oliver Purschke, Koenraad Martens, Arnaud Pocheville, Jonas O. Wolff, Boris R. Krasnov, Peter Poschlod, Ricardo Rocha, Peter Meffert, Domingo Flores Hernandez, Rebecca Spake, A. Cormont, Janet Higuti, Rodrigo Assis de Carvalho, Eric Le Saux, Natalie Robinson, Zoë Lindo, Fábio Z. Farneda, Honor C. Prentice, Bryndís Marteinsdóttir, German Centre for Integrative Biodiversity Research, German Research Foundation, Fédération Île de France de Recherche en Environnement, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Swiss National Science Foundation, Centre d'Ecologie et des Sciences de la COnservation (CESCO), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences [Moscow] (RAS), Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Alice Holt Research Station, Forest Research [Great Britain], University of South Bohemia, Grup de Recerca 'Freshwater Biology and Management' (FEM), Universitat de Barcelona (UB), Severtsov Institute of Ecology and Evolution, Biodiversité, Gènes et Communautés, Institut National de la Recherche Agronomique (INRA), Forest Research, Institut Français de Recherche pour l'Exploitation de la Mer - Nantes (IFREMER Nantes), Université de Nantes (UN), Centre for Functional Ecology, University of Coimbra [Portugal] (UC), Laboratoire d'Ecologie Alpine (LECA), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Naturalis Biodiversity Center [Leiden], Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Applied and Restoration Ecology Group, Pyrenean Institute of Ecology, Department of Ecology, State University of Maringá, Ramon Science Center and Mitrani Department of Desert Ecology, Ben-Gurion University of the Negev (BGU), University of Western Ontario (UWO), Aquatic and Terrestrial Ecology, Operational Directorate Natural Environment, Royal Belgian Institute of Natural Sciences (RBINS), Ecosystèmes lagunaires : organisation biologique et fonctionnement (ECOLAG), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Muséum national d'Histoire naturelle (MNHN)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), The University of Sydney, Universität Regensburg (REGENSBURG), Universität Regensburg, Lund University [Lund], German Centre for Integrative Biodiversity Research (iDiv), Laboratoire des Sciences de l'Environnement, Universitat Pompeu Fabra [Barcelona], Institute of Zoology, Slovak Academy of Science [Bratislava] (SAS), Université de Montpellier (UM), Peace Research Institute Frankfurt (PRIF), Hessische Stiftung Friedens- und Konfliktforschung, Martin-Luther-University Halle-Wittenberg, Universität Zürich [Zürich] = University of Zurich (UZH), University of New England (UNE), Institute of Ecology and Evolution [Bern, Switzerland], University of Bern, Dynamiques et écologie des paysages agriforestiers (DYNAFOR), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre for Ecosystems, Society and Biosecurity, Forest Research, Alice Holt Lodge, Biology Centre of the Czech Academy of Sciences (BIOLOGY CENTRE CAS), Czech Academy of Sciences [Prague] (CAS), Ural Branch of Russian Academy of Sciences (UB RAS), Institute of Plant and Animal Ecology, Institut de Recerca de la Biodiversitat - Biodiversity Research Institute [Barcelona, Spain] (IRBio UB), Department de Biologia Evolutiva, Ecologia i Ciències Ambientals, University of Barcelona, Écologie et Modèles pour l'Halieutique (IFREMER EMH), Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Universidade Estadual de Goiás (UEG), University of Bielsko-Biala, Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), ARC Centre of Excellence for Coral Reef Studies (CoralCoE), James Cook University (JCU), Centro de Estudos do Ambiante e do Mar (CESAM), Universidade de Aveiro, National Penghu University of Science and Technology, Wageningen University and Research [Wageningen] (WUR), Wageningen Environmental Research, Evolution and Ecology Research Centre [UNSW Sydney], School of Biological, Earth and Environmental Sciences [Sydney] (BEES), University of New South Wales [Sydney] (UNSW)-University of New South Wales [Sydney] (UNSW), Laboratorio de Ictiología, Instituto Nacional de Limnología [Santa Fe] (INALI), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET)-Universidad Nacional del Litoral [Santa Fe] (UNL)-Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET)-Universidad Nacional del Litoral [Santa Fe] (UNL), Universidade Estadual de Maringà (UEM), Institute of Environmental Sciences [Leiden] (CML), Leiden University, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS), SUNY College of Environmental Science and Forestry (SUNY-ESF), State University of New York (SUNY), Dresden University of Applied Sciences, OBG, Part of Ramboll, The Natural History Museum [London] (NHM), State University of Rio de Janeiro, Smithsonian Tropical Research Institute, Centre for Ecology - Evolution and Environmental Changes (cE3c), Universidade de Lisboa (ULISBOA), Universidad Autónoma de Campeche, Institut de Recherches en Biologie Végétale [Montréal] (IRBV), Université de Montréal (UdeM), Unité entomologie et plantes invasives, Laboratoire de la Santé des Végétaux, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Pyrenean Institute of Ecology (IPE), Spanish National Research Council (CSIC), La Trobe University [Melbourne], Rural Federal University of Pernambuco, The Jacob Blaustein Institutes for Desert Research (BIDR), Mitrani Department of Desert Ecology, Jacob Blaustein Institute for Desert Research, Museu de Ciencies Naturals de Granollers, Macquarie University [Sydney], The soil conservation service of Iceland (SCSI), Universiteit Gent = Ghent University [Belgium] (UGENT), Imprint, Universidad de Murcia, Departamento de Ecología e Hidrología. Facultad de Biología. Universidad de Murcia. 30100 Murcia. Spain, Max Planck Institute for Ornithology, Max-Planck-Gesellschaft, University of Salford, School of Environmental and Life Sciences, MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), The James Hutton Institute, Istanbul Technical University (ITÜ), Avrasya Yer Bilimleri Enstitüsü = Eurasia Institute of Earth Sciences [Istanbul] (AYBE), CREAF - Centre for Ecological Research and Applied Forestries, Evolution et Diversité Biologique (EDB), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Swiss Federal Insitute of Aquatic Science and Technology [Dübendorf] (EAWAG), University of Regensburg, Department of Ecology and Conservation Biology, Department of Physical Geography and Ecosystem Science [Lund], Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Tallinn University of Technology (TTÜ), Universitat Pompeu Fabra [Barcelona] (UPF), Institute of Evolutionary Biology (UPF-CSIC), CEXS-UPF-PRBB, National ecological observatory network, University of Colorado [Boulder], Department of Ecology and Evolutionary Biology [Boulder], University of Southampton, Providence University, School of Geography and Environmental Sciences, University of Lódź, Institute of Parasitology [České Budějovice] (BIOLOGY CENTRE CAS), Czech Academy of Sciences [Prague] (CAS)-Czech Academy of Sciences [Prague] (CAS), Institute of Zoology Slovak Academy of Sciences, Centro de Biologia Aquática, Escola de Ciências Agrárias e Biológicas, Pontifícia Universidade Católica de Goiás, Biometris, University of Connecticut (UCONN), Fenner School of Environment and Society, Australian National University (ANU), This work was funded by the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig (funded by the German Research Foundation, DFG FZT 118). We thank Jitendra Gaikwad for his precious help with the technical aspects of the database storage and management through the iDiv Biodiversity Portal (https://idata.idiv.de/, https://doi.org/10.25829/idiv.286-21-2695) and the iDiv IT Support for their help in the CESTES website development (https://icestes.github.io/).The datasets provided by A. Jeliazkov were collected with financial support from the Federation d'Ile-de-France pour la Recherche en Environnement (FIRE FR-3020). The dataset provided by R. Carvalho and F.L. Tejerina-Garro had financial support from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq No. 471283/2006-1) granted to FLTG. The datasets provided by R. van Klink, J.-Y. Humbert, R. Arlettaz and M.H. M. Menz were collected with financial support from the Swiss National Science Foundation (grants 31003A_125398/2 and 31003A_149656) awarded to RA., Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), École nationale supérieure agronomique de Toulouse (ENSAT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Écologie et Modèles pour l'Halieutique (EMH), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centre for Functional ecology, Universiteit Leiden, Universidade de Lisboa = University of Lisbon (ULISBOA), Unité entomologie et plantes invasives (LSV Montpellier), Laboratoire de la santé des végétaux (LSV), Instituto Pirenaico de Ecologìa = Pyrenean Institute of Ecology [Zaragoza] (IPE - CSIC), Universiteit Gent = Ghent University (UGENT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Subjects: 0106 biological sciences, Biodiversity, DIVERSITY, computer.software_genre, 01 natural sciences, VARIABLES, Aardobservatie en omgevingsinformatica, Biodiversiteit en Beleid, Taxonomic rank, PLANT FUNCTIONAL TRAITS, lcsh:Science, Macroecology, ComputingMilieux_MISCELLANEOUS, Database, Ecology, Statistics, Community structure, Plants, PE&RC, Biota, Computer Science Applications, Geography, Biometris, Trait, LIFE-HISTORY TRAITS, Statistics, Probability and Uncertainty, COMMUNITY STRUCTURE, Information Systems, Metacommunity, Statistics and Probability, Earth Observation and Environmental Informatics, Life on Land, Ecology (disciplines), BIOLOGICAL TRAITS, MODELS, Library and Information Sciences, 010603 evolutionary biology, Biodiversity and Policy, Life history theory, Education, Database management, ddc:570, Animals, Life Science, Community ecology, Author Correction, Gestió de bases de dades, SPERMONDE-ARCHIPELAGO, Community, 010604 marine biology & hydrobiology, 15. Life on land, Biodiversitat, Research data, Earth and Environmental Sciences, Dades de recerca, lcsh:Q, Probability and Uncertainty, BIODIVERSITY, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, computer, RESPONSES
Abstract: The use of functional information in the form of species traits plays an important role in explaining biodiversity patterns and responses to environmental changes. Although relationships between species composition, their traits, and the environment have been extensively studied on a case-by-case basis, results are variable, and it remains unclear how generalizable these relationships are across ecosystems, taxa and spatial scales. To address this gap, we collated 80 datasets from trait-based studies into a global database for metaCommunity Ecology: Species, Traits, Environment and Space; “CESTES”. Each dataset includes four matrices: species community abundances or presences/absences across multiple sites, species trait information, environmental variables and spatial coordinates of the sampling sites. The CESTES database is a live database: it will be maintained and expanded in the future as new datasets become available. By its harmonized structure, and the diversity of ecosystem types, taxonomic groups, and spatial scales it covers, the CESTES database provides an important opportunity for synthetic trait-based research in community ecology., This work was funded by the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig (funded by the German Research Foundation; DFG FZT 118). We thank Jitendra Gaikwad for his precious help with the technical aspects of the database storage and management through the iDiv Biodiversity Portal (https://idata.idiv.de/, https://doi.org/10.25829/idiv.286-21-2695) and the iDiv IT Support for their help in the CESTES website development (https://icestes.github.io/). The datasets provided by A. Jeliazkov were collected with financial support from the Fédération d’Ile-de-France pour la Recherche en Environnement (FIRE FR-3020). The dataset provided by R. Carvalho and F.L. Tejerina-Garro had financial support from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq No. 471283/2006-1) granted to FLTG. The datasets provided by R. van Klink, J.-Y. Humbert, R. Arlettaz and M.H.M. Menz were collected with financial support from the Swiss National Science Foundation (grants 31003A_125398/2 and 31003A_149656) awarded to RA.
Published: 2020
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41. Formation of S0s in extreme environments : I: clues from kinematics and stellar populations

Author: Bruno Rodríguez del Pino, Boris Haeussler, Karín Menéndez-Delmestre, Claudia Mendes de Oliveira, Arianna Cortesi, Michael R. Merrifield, Evelyn J. Johnston, Yara L. Jaffé, Ana L. Chies-Santos, Yun-Kyeong Sheen, Lodovico Coccato, Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737, Jaffé, Y. L. [0000-0003-2150-1130], Cortesi, A. [0000-0002-0620-136X], Chies Santos, A. [0000-0003-3220-0165], Mendes de Oliveira, C. [0000-0002-5267-9065], Johnston, E. [0000-0002-2368-6469], Merrifield, M. [0000-0002-4202-4727], Rodríguez del Pino, B. [0000-0001-5171-3930], Ministerio de Economía y Competitividad (MINECO), Comisión Nacional de Investigación Científica y Tecnológica (CONICYT), Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT), National Research Foundation of Korea (NRF), Agencia Estatal de Investigación (AEI), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Rio Grande do Sul Research Foundation (FAPERGS), European Southern Observatory under ESO programme, FONDECYT Iniciacion 2018, and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) and the Rio Grande do Sul Research Foundation (FAPERGS)
Subjects: Kinematics, Metallicity, FOS: Physical sciences, Astrophysics, Astrophysics::Cosmology and Extragalactic Astrophysics, Formation [Galaxies], cD, elliptical and lenticular [Galaxies], Populacoes estelares, Extreme environment, Cinemática, Stellar populations, Formacao de estrelas, Lenticular galaxy, Astrophysics::Galaxy Astrophysics, Kinematics and dynamics [Galaxies], Physics, Elliptical and lenticular, cD [Galaxies], Star formation, Astronomy and Astrophysics, Astrophysics - Astrophysics of Galaxies, Galaxy, Ram pressure, Space and Planetary Science, Astrophysics of Galaxies (astro-ph.GA), Galaxias lenticulares
Abstract: Despite numerous efforts, it is still unclear whether lenticular galaxies (S0s) evolve from spirals whose star formation was suppressed, or formed trough mergers or disk instabilities. In this paper we present a pilot study of 21 S0 galaxies in extreme environments (field and cluster), and compare their spatially-resolved kinematics and global stellar populations. Our aim is to identify whether there are different mechanisms that form S0s in different environments. Our results show that the kinematics of S0 galaxies in field and cluster are, indeed, different. Lenticulars in the cluster are more rotationally supported, suggesting that they are formed through processes that involve the rapid consumption or removal of gas (e.g. starvation, ram pressure stripping). In contrast, S0s in the field are more pressure supported, suggesting that minor mergers served mostly to shape their kinematic properties. These results are independent of total mass, luminosity, or disk-to-bulge ratio. On the other hand, the mass-weighted age, metallicity, and star formation time-scale of the galaxies correlate more with mass than with environment, in agreement with known relations from previous work such as the one between mass and metallicity. Overall, our results re-enforce the idea that there are multiple mechanisms that produce S0s, and that both mass $and$ environment play key roles. A larger sample is highly desirable to confirm or refute the results and the interpretation of this pilot study., 18 pages, 11 figure, accepted for publication in MNRAS
Published: 2020

42. Towards on-demand I/O forwarding in HPC platforms

Author: Alberto Miranda, Toni Cortes, Francieli Zanon Boito, Philippe O. A. Navaux, Ramon Nou, Jean Luca Bez, Universitat Politècnica de Catalunya. Departament d'Arquitectura de Computadors, Universitat Politècnica de Catalunya. CAP - Grup de Computació d'Altes Prestacions, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Topology-Aware System-Scale Data Management for High-Performance Computing (TADAAM), Laboratoire Bordelais de Recherche en Informatique (LaBRI), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), Barcelona Supercomputing Center - Centro Nacional de Supercomputacion (BSC - CNS), Université polytechnique de Catalogne (UPC), This study was financed by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) - Finance Code 001. It has also received support from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil, It is also partially supported by the Spanish Ministry of Economy and Competitiveness (MINECO) under grants PID2019-107255GB, and the Generalitat de Catalunya under contract 2014–SGR–1051., Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Inria Bordeaux - Sud-Ouest, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), and BOITO, Francieli Zanon
Subjects: I/O forwarding, Computer science, Layout, 02 engineering and technology, 01 natural sciences, 010104 statistics & probability, Hardware, Bandwidth, Supercomputadors, [INFO.INFO-DC] Computer Science [cs]/Distributed, Parallel, and Cluster Computing [cs.DC], 0202 electrical engineering, electronic engineering, information engineering, 0101 mathematics, Layer (object-oriented design), Informàtica::Arquitectura de computadors::Arquitectures paral·leles [Àrees temàtiques de la UPC], 020203 distributed computing, Focus (computing), business.industry, Bandwidth (signal processing), Message systems, Control reconfiguration, Random access memory, Supercomputers, Program processors, Parallel processing (DSP implementation), Software deployment, Reconfiguration, Resource allocation, [INFO.INFO-DC]Computer Science [cs]/Distributed, Parallel, and Cluster Computing [cs.DC], Software architecture, business, Computer network
Abstract: I/O forwarding is an established and widely-adopted technique in HPC to reduce contention and improve I/O performance in the access to shared storage infrastructure. On such machines, this layer is often physically deployed on dedicated nodes, and their connection to the clients is static. Furthermore, the increasingly heterogeneous workloads entering HPC installations stress the I/O stack, requiring tuning and reconfiguration based on the applications' characteristics. Nonetheless, it is not always feasible in a production system to explore the potential benefits of this layer under different configurations without impacting clients. In this paper, we investigate the effects of I/O forwarding on performance by considering the application's I/O access patterns and system characteristics. We aim to explore when forwarding is the best choice for an application, how many I/O nodes it would benefit from, and whether not using forwarding at all might be the correct decision. To gather performance metrics, explore, and understand the impact of forwarding I/O requests of different access patterns, we implemented FORGE, a lightweight I/O forwarding layer in user-space. Using FORGE, we evaluated the optimal forwarding configurations for several access patterns on MareNostrum 4 (Spain) and Santos Dumont (Brazil) supercomputers. Our results demonstrate that shifting the focus from a static system-wide deployment to an on-demand reconfigurable I/O forwarding layer dictated by application demands can improve I/O performance on future machines. This study was financed by the Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. It has also received support from the Conselho Nacional de Desenvolvimento Científico e Tecnologico (CNPq), Brazil; It is also partially supported by the Spanish Ministry of Economy and Competitiveness (MINECO) under grants PID2019-107255GB; and the Generalitat de Catalunya under contract 2014–SGR–1051.
Published: 2020

43. Reduced toxicity of 3-epi-deoxynivalenol and de-epoxy-deoxynivalenol through deoxynivalenol bacterial biotransformation

Author: Wulf-Dieter Moll, Ana Paula Frederico Rodrigues Loureiro Bracarense, Juliana Rubira Gerez, Gerd Schatzmayr, Philippe Pinton, Isabelle P. Oswald, Ting Zhou, Alix Pierron, Laboratory of Animal Pathology, State University of Londrina = Universidade Estadual de Londrina, Biosynthèse & Toxicité des Mycotoxines (ToxAlim-BioToMyc), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BIOMIN Research Center, Agriculture and Agri-Food [Ottawa] (AAFC), This work was partially financed by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brazil and Comite Frances de Avaliacao da Cooperacao Universitaria com o Brasil (COFECUB), France, CAPES/COFECUB Program (number 0389/2019) and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil (number 308136/2018-7)., Biomin Research Center, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil, Comitê Francês de Avaliação da Cooperação Universitária com o Brasil (COFECUB), CAPES/COFECUB Program (number 0389/2019), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil (number 308136/2018-7)
Subjects: Male, medicine.medical_specialty, Lymphoid Tissue, Swine, [SDV]Life Sciences [q-bio], [SDV.TOX.TVM]Life Sciences [q-bio]/Toxicology/Vegetal toxicology and mycotoxicology, Histopathology, Immunoglobulins, Food Contamination, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, Biology, Toxicology, Occludin, Weight Gain, Microbiology, Immunomodulation, 03 medical and health sciences, chemistry.chemical_compound, 0404 agricultural biotechnology, Biotransformation, Detoxification, Intestine, Small, medicine, Ingestion, Animals, Mycotoxin, Adverse effect, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, food and beverages, 04 agricultural and veterinary sciences, General Medicine, Organ Size, Mycotoxins, 040401 food science, Animal Feed, 3. Good health, chemistry, Liver, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Toxicity, Cytokines, Trichothecenes, Food Science
Abstract: International audience; Ingestion of deoxynivalenol (DON), one of the most common mycotoxin contaminants of cereals, leads to adverse effects for animal and human health. Bacterial biotransformation is a strategy to mitigate the toxicity of this mycotoxin. The present study aims to evaluate the toxicity of two bacterial biotranformation products of DON: 3-epi-deoxynivalenol (3-epi-DON) and de-epoxy-deoxynivalenol (DOM-1) through zootechnical, hematological, histological and immunological assays. Twenty-four 4weeks-old piglets received a control diet or a diet contaminated with 3 mg kg-1 DON, DOM-1, or 3-epi-DON for 7 days. Sample tissues were collected for histomorphometrical analysis, expression of cytokines and cell protein junctions. The zootechnical and hematological parameters were not modulated by any treatment. Ingestion of DON induced histological alterations in the intestine, liver and lymphoid organs, as well as an overexpression of pro-inflammatory cytokines, E-cadherin and occludin. These changes were not observed in piglets receiving the DOM-1 and 3-epi-DON contaminated diets. Pigs fed 3-epi-DON contaminated diet showed an increase in IgM levels in comparison with other diets, while no change was observed in IgA and IgG levels among the diets. Our results indicate that DOM-1 and 3-epi-DON are not toxic for piglets; thus bacterial biotransformation seems to be a sustainable alternative to reduce mycotoxin toxicity.
Published: 2019
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44. The selenium-binding protein of Theobroma cacao: A thermostable protein involved in the witches’ broom disease resistance

Author: Fabienne Micheli, Karina Peres Gramacho, Carlos Priminho Pirovani, Eline Matos Lima, Monaliza Macêdo Ferreira, Sara Pereira Menezes, Bruno Silva Andrade, Akyla Maria Martins Alves, Universidade Estadual De Santa Cruz [Brazil] (UESC), Cocoa Research Center CEPEC-CEPLAC, Universidade Estadual do Sudoeste da Bahia (UESB), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), and Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB)DTE0038/2013
Subjects: 0106 biological sciences, 0301 basic medicine, Physiology, [SDV]Life Sciences [q-bio], Moniliophthora, Selenium-Binding Proteins, Plant Science, 01 natural sciences, Moniliophthora perniciosa, F30 - Génétique et amélioration des plantes, Gene expression, Expression des gènes, Selenium binding, ComputingMilieux_MISCELLANEOUS, Disease Resistance, Plant Proteins, Reverse Transcriptase Polymerase Chain Reaction, Circular Dichroism, 3. Good health, Molecular Docking Simulation, Biochemistry, Molecular docking, maladie du balai de sorcière, Biology, 03 medical and health sciences, Selenium, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Theobroma cacao, Computer Simulation, Homology modeling, Gene, H20 - Maladies des plantes, Plant Diseases, Cacao, cDNA library, Biotic stress, Résistance aux maladies, biology.organism_classification, 030104 developmental biology, Agaricales, 010606 plant biology & botany
Abstract: International audience; The selenium-binding proteins are known to be inducers of apoptosis in human and animals, and have been studied as target for the treatment of various types of cancer. In plants, SBP expression has been related to abiotic and biotic stress resistance. The SBP from Theobroma cacao (TcSBP) was first identified from a cocoa-Moniliophthora perniciosa cDNA library. The present study provides details on the TcSBP gene and protein structure. Multiple alignments revealed conserved domains between SBP from plants, human and archea. Homology modeling and molecular docking were performed and showed that the TcSBP has affinity to selenite in the active CSSC site. This result was confirmed by circular dichroism of the recombinant TcSBP, which also presented thermostable behavior. RT-qPCR analysis showed that TcSBP was differentially expressed in resistant vs susceptible cacao varieties inoculated by M. perniciosa and its expression was probably due to hormone induction via cis-regulating elements present in its promotor. The presence of the CSSC domain suggested that TcSBP acted by altering oxidation/reduction of proteins during H2O2 production and programmed cell death in the final stages of the witches' broom disease. To our knowledge, this is the first in silk and in vitro analysis of the SBP from cacao.
Published: 2019
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45. Involvement of structurally distinct cupuassu chitinases and osmotin in plant resistance to the fungus Moniliophthora perniciosa

Author: Karina Peres Gramacho, Fabienne Micheli, Rafael Moysés Alves, Lucilia Helena Marcellino, Raner José Santana Silva, Universidade Estadual De Santa Cruz [Brazil] (UESC), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Cocoa Research Center CEPEC-CEPLAC, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB)DTE0038/2013, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)
Subjects: 0106 biological sciences, 0301 basic medicine, Physiology, [SDV]Life Sciences [q-bio], Moniliophthora, Chitin, Plant Science, 01 natural sciences, Moniliophthora perniciosa, F30 - Génétique et amélioration des plantes, chemistry.chemical_compound, Arabidopsis, Witches' broom disease, Expression des gènes, Chitosanase, Disease Resistance, Plant Proteins, 2. Zero hunger, biology, Chitinases, food and beverages, Pathogenesis-related proteins, Molecular Docking Simulation, Molecular docking, Brazil, maladie du balai de sorcière, Pouvoir pathogène, Theobroma grandiflorum [EN], Chitine, Microbiology, 03 medical and health sciences, Chitosane, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, H20 - Maladies des plantes, Chitosan, Cacao, Abiotic stress, fungi, biology.organism_classification, 030104 developmental biology, chemistry, Chitinase, biology.protein, Theobroma grandiflorum, Gene expression, Agaricales, 010606 plant biology & botany
Abstract: International audience; The cupuassu tree (Theobroma grandiflorwn) is a crop of great economic importance to Brazil, mainly for its pulp and seeds, which are used in food industry. However, cupuassu fruit production is threatened by witches' broom disease caused by the fungus Moniliophthora perniciosa. As elements of its defense mechanisms, the plant can produce and accumulate pathogenesis-related (PR) proteins such as chitinases and osmotins. Here, we identified three cupuassu PR proteins (TgPR3, TgPR5 and TgPR8) from cupuassu-M. perniciosa interaction RNA-seq data. TgPR3 and TgPR8 corresponded to chitinases, and TgPR5 to osmotin; they are phylogenetically related to cacao and to Arabidopsis PR sequences involved in biotic and abiotic stress. The TgPR proteins' tridimensional structure was obtained through homology modeling, and molecular docking with chitin and chitosan showed that the TgPR proteins can interact with both cell wall molecules and presented a higher affinity for chitosan. TgPR gene expression was analyzed by RT-qPCR on resistant and susceptible cupuassu genotypes infected by M. perniciosa at 8, 24, 48 and 72 h after infection (hai). The TgPR genes showed higher expression in resistant plants compared to the susceptible ones, mainly for TgPR5 at 8 and 24 hai, while the expression was lower in the susceptible cupuassu plants. To our knowledge, this is the first in silico and in vitro reports of cupuassu PR protein. The data suggested that TgPRs could be involved in recognizing mechanisms of the plant's innate immune system through chitin receptors. Our results also suggest a putative role of chitinase/chitosanase for the TgPR5/osmotin.
Published: 2019
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46. Escalation and Morphological Constraints of Antagonistic Armaments in Water Striders

Author: Crumière, Antonin Jean Johan, Armisen, David, Vargas-Lowman, Aïdamalia, Kubarakos, Martha, Moreira, Felipe Ferraz Figueiredo, Khila, Abderrahman, Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq-PVE) 400751/2014-3, Centre d'Etude de la Biodiversite Amazonienne CEBA: ANR-10-LABX-25-01, European Project: 616346,EC:FP7:ERC,ERC-2013-CoG,WATERWALKING(2014), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), and Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA)
Subjects: antagonistic armaments, Ecology, arms-race, conflict, [SDV]Life Sciences [q-bio], [SDV.BA]Life Sciences [q-bio]/Animal biology, lcsh:Evolution, selection, water striders, Environmental Sciences & Ecology, secondary sexual character, sexual conflict, lcsh:QH540-549.5, sexual dimorphism, coevolution, evolution, lcsh:QH359-425, lcsh:Ecology, waterstrider, dimorphism, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS, heteroptera, trade-off
Abstract: International audience; Sexual conflict may result in the escalating coevolution of sexually antagonistic traits. However, our understanding of the evolutionary dynamics of antagonistic traits and their role in association with sex-specific escalation remains limited. Here we study sexually antagonistic coevolution in a genus of water striders called Rhagovelia. We identified a set of male grasping traits and female anti-grasping traits used during pre-mating struggles and show that natural variation of these traits is associated with variation in mating performance in the direction expected for antagonistic coevolution. Phylogenetic mapping detected signal of escalation of these sexually antagonistic traits suggesting an ongoing arms race. Moreover, their escalation appears to be influenced by a trade-off with dispersal through flight in both sexes. Altogether our results highlight how sexual interactions and natural selection may have shaped sex-specific antagonistic trait coevolution.
Published: 2019
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47. HVA22 from citrus: A small gene family whose some members are involved in plant response to abiotic stress

Author: Raner José Santana Silva, Jacqueline Araújo Castro, Fabienne Micheli, Monique Drielle Gomes Ferreira, Universidade Estadual De Santa Cruz [Brazil] (UESC), Instituto Federal Baiano (IFBaiano), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), and CNPq Universal project 471742/2013-9
Subjects: 0106 biological sciences, Phylogénie, Citrus, Arabidopsis thaliana, Physiology, Stress abiotique, [SDV]Life Sciences [q-bio], Plant Science, In silico analysis, 01 natural sciences, F30 - Génétique et amélioration des plantes, Gene Expression Regulation, Plant, Gene duplication, Gene expression, Expression des gènes, Citrus clementina, cis-elements, Promoter Regions, Genetic, Phylogeny, Plant Proteins, 2. Zero hunger, Genetics, 0303 health sciences, Dehydration, U10 - Informatique, mathématiques et statistiques, food and beverages, Plants, Genetically Modified, Transmembrane domains, Modèle mathématique, Citrus × sinensis, Citrus sinensis, F60 - Physiologie et biochimie végétale, Biology, Genes, Plant, 03 medical and health sciences, Bioinformatique, Stress, Physiological, Tobacco, Gene family, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Gene, 030304 developmental biology, Drought, Abiotic stress, Alternative splicing, Transgenic tobacco, Hydrogen Peroxide, Résistance à la sécheresse, Gène, Hordeum vulgare, Transcriptome, 010606 plant biology & botany
Abstract: International audience; The HVA22 gene has been isolated for the first time from the aleurone layer of barley (Hordeum vulgare). Here, we characterized the HVA22 family from citrus (C. clementina and C. sinensis). Twelve genes, 6 in each species, were identified as well as duplication events for some of them. The ORF size ranged from 235 to 804 bp and the protein molecular weight from 94 to 267 kDa. All the citrus HVA22 protein presented transmembrane location and conserved TB2/DP1/HVA22 region. Phylogenetic and gene expression analyses suggested that some citrus HVA22 play a role in flower and fruit development, and that gene expression may be regulated by hormone or environmental conditions. Other regulation levels were also predicted, such as alternative splicing and post translational modifications. The overall data indicated that citrus HVA22 may be involved in vesicular traffic in stressed cells, and that CcHVA22d could be involved in dehydration tolerance.
Published: 2019
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48. 3D characterisation of the thermohaline structure in the southwestern tropical Atlantic derived from functional data analysis of in situ profiles

Author: Ramilla V. Assunção, Bernard Bourlès, Moacyr Araujo, J.-F. Ternon, Carlos Henrique S. Silva, Alex Costa da Silva, Amédée Roy, Arnaud Bertrand, Universidade Federal de Pernambuco [Recife] (UFPE), MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Instrumentation, Moyens analytiques, Observatoires en Géophysique et Océanographie (IMAGO), Universidade Federal Rural de Pernambuco (UFRPE), We acknowledge the French oceanographic fleet for funding the atsea survey and the officers and crew of the R/V Antea for their contribution to the success of the operations during the ABRACOS cruises. We thanks the Helmholtz-Zentrum fur Ozeanforschung Kiel (GEOMAR) for PANGEA data availability. Thanks to Daniel Grados (Instituto del Mar del Peru) for support with programming and Functional Data Analysis. The Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) by supported through a PhD scholarship grant for Assuncao, Ramilla. M.A. thanks the support of the Brazilian Research network on Global Climate Change FINEP/Rede CLIMA (grants 01.13.0353-00). This work is a contribution to the International Joint Laboratory TAPIOCA (www.tapioca.ird.fr), CAPES/COFECUB program (88881.142689/2017-01), PADDLE project (funding by the European Union's Horizon 2020 research and innovation programme -grant agreement No. 73427)., Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, Water mass, 010504 meteorology & atmospheric sciences, Mixed layer, Western boundary current, Aquatic Science, Tropical Atlantic, 01 natural sciences, salinity, decadal changes, Barrier layer, Thermocline, Photic zone, 14. Life underwater, 0105 earth and related environmental sciences, ACL, 010604 marine biology & hydrobiology, Intertropical Convergence Zone, seasonal variations, Geology, ocean, Boundary current, Oceanography, Ocean stratification, 13. Climate action, south atlantic, Thermohaline circulation, western boundary circulation, water masses, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, North Brazilian Undercurrent system
Abstract: International audience; The dynamic of the thermohaline structure of the upper ocean, which depends on ocean-atmosphere interactions, drives most near surface oceanic processes, including the control of gases and heat fluxes, and nutrient availability in the photic layer. The thermohaline structure of the southwestern tropical Atlantic (SWTA), a key region for diagnosing variation of the Atlantic Meridional Overturning Circulation, has prime impact on global climate. Characterising the thermohaline structure is typically based on the application of classical statistical methods on vertical profiles. Such approach has important limitations since classical methods do not explicitly contemplate the vertical nature of the profiles. Functional Data Analysis (FDA) is a new alternative to solve such drawbacks. Here, we apply an FDA approach to characterise the 3D canonical thermohaline structure of the SWTA in austral spring and fall. Our results reveal a clear spatial pattern with the presence of three areas with significantly different thermohaline structure. Area 1, mostly located along the continental slope, reflects the western boundary current system, with low static stability and high frequency of occurrence of barrier layer (BL). Conversely, Area 2, located along the Fernando de Noronha chain, presents strong static stability with a well-marked thermocline. This area, under the influence of the eastern Atlantic, is characterised by a low BL frequency, which is seasonally modulated by the latitudinal oscillation of the Intertropical Convergence Zone, controlling the regime of precipitation. In turn, Area 3 behaves as a transition zone between A1 and A2 with the presence of the water core of maximum salinity in subsurface, and therefore presence of strong-moderate BL. Beyond this study, FDA approach emerges as a powerful way to describe, characterise, classify and compare ocean patterns and processes. It can be applied to in situ data but could also be used to deeply and comprehensively explore ocean model output.
Published: 2020
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49. Seasonal variation in natural mortality factors of Tuta absoluta (Lepidoptera: Gelechiidae) in open-field tomato cultivation

Author: Mateus R. Campos, Marcelo Coutinho Picanço, Ézio Marques da Silva, Marianne A. Soares, Leandro Bacci, Júlio Cláudio Martins, Universidade Federal de Sergipe (DFI/CCET/), Universidade Federal de Vicosa (UFV), Instituto Federal de Educação, Ciência e Tecnologia Baiano, Partenaires INRAE, Dept Entomol., University of Wisconsin-Madison, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Université de Nice Sophia-Antipolis (UNSA), National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq), Brazilian Federal Agency for the Support and Evaluation of Graduate Education (Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior-CAPES), and Minas Gerais State Foundation for Research Aid (Fundacao de Amparo a Pesquisa do Estado de Minas Gerais-FAPEMIG)
Subjects: 0106 biological sciences, predator, [SDV]Life Sciences [q-bio], Tomato borer, 01 natural sciences, Insect seasonality, Parasitoid, Physiological disturbance, Lepidoptera genitalia, medicine, Predator, parasitoid, 2. Zero hunger, biology, Ecology, fungi, physiological disturbance, climatic elements, insect seasonality, 15. Life on land, Seasonality, Gelechiidae, biology.organism_classification, medicine.disease, 010602 entomology, 13. Climate action, Insect Science, Climatic elements, Mortality factors, Tuta absoluta, Agronomy and Crop Science, 010606 plant biology & botany
Abstract: International audience; The seasonal variation in natural mortality of phytophagous insects is determined by the relative importance of biotic and abiotic factors in agroecosystems. Knowledge regarding these factors throughout the year represents a key concern for IPM programmes. Seasonal population fluctuations of tomato pinworm, Tuta absoluta, led to an investigation of its natural mortality factors during the rainy season when the population level is low and during the dry season when population peaks occur. The aim of this study was to verify the seasonal variation in T. absoluta mortality factors in tomato crops. Immature stages of T. absoluta were obtained from laboratory-rearing in the laboratory. These were taken to the field and monitored over two years. The mortality causes for each stage of insect development from egg to adult were assessed daily. Multiple biotic and abiotic mortality factors affected the immature T. absoluta stages such as rainfall, physiological disturbances, diseases, parasitoids and predators. The key T. absoluta mortality factor during summer-spring was predation. In addition, larvae predation correlated positively with temperature, wind velocity, photoperiod and rainfall. Nevertheless, during winter-fall, the key mortality factor was parasitism. Therefore, the critical stage for mortality was 3rd- and 4th-instar larvae, being more vulnerable to natural control factors. Finally, the results showed the importance of vertical and horizontal action on natural mortality factors.
Published: 2019
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50. Spring is coming: genetic analyses of the bud break date locus reveal candidate genes from the cold perception pathway to dormancy release in apple (Malus x domestica Borkh.)

Author: Marcus Vinícius Kvitschal, Amanda Malvessi Cattani, Tiago Sartor, Carolina Tessele, Carla Andréa Delatorre, Marcos Mota do Carmo Costa, Luís Fernando Revers, Roberto C. Togawa, Priscila Grynberg, Sérgio Amorim de Alencar, Vanessa Buffon, Vítor da Silveira Falavigna, Diogo Denardi Porto, Frederico Denardi, Orzenil B. Silva-Junior, Paulo Ricardo Dias de Oliveira, Yohanna Evelyn Miotto, Georgios J. Pappas, Ana Beatriz Costa Czermainski, YOHANNA EVELYN MIOTTO, UFRGS, CAROLINA TESSELE, UFRGS, ANA BEATRIZ COSTA CZERMAINSKI, CNPUV, DIOGO DENARDI PORTO, CPATSA, VÍTOR DA SILVEIRA FALAVIGNA, UFRGS, TIAGO SARTOR, UFRGS, AMANDA MALVESSI CATTANI, UFRGS, CARLA ANDREA DELATORRE, UFRGS, SÉRGIO AMORIM DE ALENCAR, UCB, ORZENIL BONFIM DA SILVA JUNIOR, Cenargen, ROBERTO COITI TOGAWA, Cenargen, MARCOS MOTA DO CARMO COSTA, Cenargen, GEORGIOS JOANNIS PAPPAS JÚNIOR, UNB, PRISCILA GRYNBERG, Cenargen, PAULO RICARDO DIAS DE OLIVEIRA, CNPUV, MARCUS VINÍCIUS KVITSCHAL, EPAGRI, FREDERICO DENARDI, EPAGRI, VANESSA BUFFON, CNPUV, LUÍS FERNANDO REVERS, UFRGS., Universidade Federal do Rio Grande do Sul (UFRGS), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universidade Católica de Brasília (UCB), Universidade de Brasília (UnB), Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Financiadora de Estudos e Projetos (FINEP) : 0107009700, Empresa Brasileira de Pesquisa Agropecuaria (Embrapa) : 02.12.12.003.00.02, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES -Ministry of Education), CAPES, CAPES : 15/2014 83, and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Ministry of Science, Technology, Innovation and Communications) : 305122/2014-2
Subjects: 0106 biological sciences, 0301 basic medicine, Candidate gene, Malus, linkage mapping, Bud dormancy, MdoPRE1, apple, Locus (genetics), Plant Science, Quantitative trait locus, Biology, lcsh:Plant culture, 01 natural sciences, 03 medical and health sciences, Annual growth cycle of grapevines, Chilling requirement, Genetic linkage, bud dormancy, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, lcsh:SB1-1110, MdoFLC, Genetics, MdoICE1, Vegetal Biology, Apples, biology.organism_classification, 030104 developmental biology, chilling requirement, Genetic marker, Linkage mapping, Dormancy, Biologie végétale, 010606 plant biology & botany
Abstract: Chilling requirement (CR) for bud dormancy completion determines the time of bud break in apple (Malus × domestica Borkh.). The molecular control of bud dormancy is highly heritable, suggesting a strong genetic control of the trait. An available Infinium II SNP platform for genotyping containing 8,788 single nucleotide polymorphic markers was employed, and linkage maps were constructed in a F1 cross from the low CR M13/91 and the moderate CR cv. Fred Hough. These maps were used to identify quantitative trait loci (QTL) for bud break date as a trait related to dormancy release. A major QTL for bud break was detected at the beginning of linkage group 9 (LG9). This QTL remained stable during seven seasons in two different growing sites. To increase mapping efficiency in detecting contributing genes underlying this QTL, 182 additional SNP markers located at the locus for bud break were used. Combining linkage mapping and structural characterization of the region, the high proportion of the phenotypic variance in the trait explained by the QTL is related to the coincident positioning of Arabidopsis orthologs for ICE1, FLC, and PRE1 protein-coding genes. The proximity of these genes from the most explanatory markers of this QTL for bud break suggests potential genetic additive effects, reinforcing the hypothesis of inter-dependent mechanisms controlling dormancy induction and release in apple trees. Made available in DSpace on 2019-06-12T00:39:12Z (GMT). No. of bitstreams: 1 fpls1000033.pdf: 2246583 bytes, checksum: 39a0aa3363675b7338cebcaa1d270aee (MD5) Previous issue date: 2019
Published: 2019
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