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Rice stands out as the most consumed cereal in the world because it is a source of vitamins, minerals, fiber, bioactive compounds, and unsaturated fatty acids. Based on this assumption, the objective of this work was to qualify and quantify fatty acids, in samples consisting of raw, integral, parboiled, and white rice marketed in southern Brazil (Vale do Itajaí) and the Vale do Pó region in northern Italy. The results of this work demonstrate that 95% of the total lipid content, in the respective rice typologies of both countries, is represented by palmitic, oleic, and linoleic acid. These results indicate that the technological processes influence the nutritional and functional quality of the rice grain in these countries. Regarding the content of total polyphenols, the maximum average value obtained was 477 g Eq g-1 for rice from both countries and the best results for antioxidant activity, using the Photoquem® method with methanolic extract, was 3.43 µM Eq Trolox g-1 for the Italian rice samples. These results indicate that the technological processes influence the nutritional and functional quality of rice grain from these countries.

This work was carried out in line with 'GP/EFSA/ENCO/380 2018/03/G04: OBEMIRISK: Knowledge platform for assessing the risk of Bisphenols on gut microbiota and its role in obesogenic phenotype: looking for biomarkers'. This research was also funded by Plan Estatal de I + D + I 2013-2016 and co-funded by FEDER-ISCIII PI17/01758, FEDER-Consejeria de Salud y Familias, Junta de Andalucia PE-0250-2019, FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades/Proyecto P18-RT-4247. All individuals participating in this research signed an informed consent form and the study protocol was approved by the Ethics Com-mittee of the University of Granada., Parabens are alkyl esters of p-hydroxybenzoic acid which are extensively used in cosmetics, pharmaceuticals and foodstuffs due to their antimicrobial properties. The most commonly used parabens are methyl-(MeP), ethyl- (EtP), propyl-(PrP) and butyl-(BuP) paraben. Most human exposure to parabens is achieved through the consumption of food or pharmaceutical products and the use of personal care products. However, studies on dietary parabens exposure and the associated factors are very scarce. The main aim of the present study was to explore factors associated with dietary exposure to parabens in Spanish adolescents according to gender. Dietary data and anthropometric measures were collected from 585 adolescents (53.4% boys) aged 12–16 years. Parabens exposure through diet was assessed using a food frequency questionnaire with food products providing more than 95% of energy and macronutrient intake being included in analysis. Stepwise regression was used to identify the foods that most contributed to parabens intake. Logistic regression was used to evaluate factors predicting higher dietary exposure to parabens. The main contributors to dietary MeP, EtP, PrP and BuP exposure in adolescent boys were eggs (41.9%), canned tuna (46.4%), bakery and baked goods products (57.3%) and pineapple (61.1%). In adolescent girls, the main contributors were apples and pears (35.3%), canned tuna (42.1%), bakery and baked goods products (55.1%) and olives (62.1%). Overweight/obese girls were more likely to belong to the highest tertile of overall parabens intake (odds ratio [OR]: 3.32; 95% confidence interval [95% CI]: 1.21–9.15) and MeP (OR: 3.05; 95% CI: 1.14–8.12) than those with a body mass index lower than 25 kg/m2. These findings suggest a positive association between dietary exposure to parabens and overweight/obesity in adolescent girls., Plan Estatal de I + D + I 2013-2016, FEDER-ISCIII PI17/01758, FEDER-Consejeria de Salud y Familias, Junta de Andalucia PE-0250-2019, FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades/Proyecto P18-RT-4247

Oxidative stability and fatty acid composition of the pumpkin seed oil (PSO) were studied, during roasting process from 80 to 150 °C and oil heat treatment (220 ˚C), by FTIR (Fourier - transform infrared) – Spectroscopy and GC/MS (Gas chromatography - mass spectrometry). The physicochemical parameters (density and iodine value) showed a significant increase (p < 0.05) during roasting, with a maximum value observed in the oil roasted at temperatures from 90 to 110 ˚C. The relative contents of polyunsaturated fatty acids (PUFAs) decreased to 87.7%, and saturated fatty acids (SFAs) increased to 112.5% at unroasted sample, after thermal treatment of oil samples. Especially at temperature 90 and 110 ˚C, oil samples have demonstrated that relative contents of PUFAs decreased to 95 % and SFAs increased to 101.6%. FTIR Spectroscopy provide to understand mechanism of chemical changes of seed during roasting process and, as result, which of obtained compounds are responsible for increasing thermal stability of oil lipids. In this study, it was observed that the best temperature for roasting pumpkin seeds is 110 ˚C, since at this point, both oxidation and Maillard reactions give rise to compounds with maximum antioxidant effect (lipid stability).

Human milk (HM) has been proven to have important and essential antioxidant properties to counteract infant susceptibility to oxidative stress. Phenolic compounds are secondary metabolites which come from plants and are potent natural antioxidants. The ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method used in the present study allowed the quantification of 26 phenolic compounds (ten hydroxybenzoic acids, seven hydroxycinnamic acids, four flavonoids, three hydroxybenzaldehydes and two other polyphenols) in HM samples at different stages of lactation (colostrum, transitional milk and mature milk) and infant formulas (IF). Many of the phenolic compounds identified have been reported to be present in HM for the first time. The total phenolic compound content (TPC) was quantified using the Folin assay and the antioxidant activity (AC) was evaluated with the DPPH, ABTS and FRAP assays. Significant differences were evidenced between HM and IF. HM from mothers with an adherence to a Mediterranean diet contained twice as many individual phenolic compounds as infant formulas, with a higher proportion of hydroxybenzoic acids. Conversely, IF showed a higher proportion of hydroxycinnamic acids. Overall, the antioxidant activity of HM showed small variations during lactation., This paper and the results presented constitute part of the thesis conducted by Silvia Sánchez Hernández within the Nutrition and Food Science Doctorate Program at the University of Granada. S. S-H. is the recipient of FPU grant (FPU15/04549) from the Ministry of Education, Culture and Sports.

Spain has 90 wines authorized as belonging to a protected designation of origin (DO). Accordingly, all wines produced in the same DO will have very similar characteristics, and, in turn, these characteristics will be different from those present in wines produced in other geographical areas. The aim of this study was to determine the preferences of a sample of consumers for Spanish red wines from different DOs. To this end, we used ISO 5495:2009 to determine consumer preference for Spanish red wines with a DO. Our tests showed that there was no specific criterion for choice of one DO over another, other than criteria based merely on marketing or historical tradition. The preferred DOs were those mainly located in northern Spain, compared with other more southern DOs. We did not find any links to other factors possibly affecting consumer preference. Our study sheds light on the preferences of a sample of tasters considered representative of the Spanish consumer of this type of wine and identified the criteria that prompt consumers to choose one wine over another. These findings would be of importance for wine exports to Spain.

Extra virgin olive oil (EVOO), water, and a water/oil mixture (W/O) were used for frying, boiling and saut&eacute, eing Mediterranean vegetables (potato, pumpkin, tomato and eggplant). Differences in antioxidant capacity (AC) (2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric iron (FRAP), 2,2-azinobis-(3-ethylbensothiazoline)-6-sulphonic acid (ABTS)), total phenolic content (TPC) and individual phenols (high-performance liquid chromatography (HPLC)) in unused and used EVOO and water were determined. The water used to boil tomatoes showed the highest TPC value, whilst the lowest was found in the EVOO from the W/O used for boiling potatoes. After processing, the concentrations of phenols exclusive to EVOO diminished to different extents. There was a greater transfer of phenols from the vegetable to the oil when eggplant, tomato and pumpkin were cooked. W/O boiling enriched the water for most of the phenols analysed, such as chlorogenic acid and phenols exclusive to EVOO. The values of AC decreased or were maintained when fresh oil was used to cook the vegetables (raw &gt, frying &gt, saut&eacute, ing &gt, boiling). The water fraction was enriched in 6-hydroxy-2,5,7,8&ndash, tetramethyl-chroman-2-carboxylic acid (Trolox) equivalents following boiling, though to a greater extent when EVOO was added. Phenolic content and AC of EVOO decreased after cooking Mediterranean diet vegetables. Further, water was enriched after the boiling processes, particularly when oil was included.

This study aimed to analyse the association between socioeconomic status (SES) and fatness and fitness in preschoolers. 2, 638 preschoolers (3–5 years old; 47.2% girls) participated. SES was estimated from the parental educational and occupational levels, and the marital status. Fatness was assessed by body mass index (BMI), waist circumference (WC), and waist-to-height ratio (WHtR). Physical fitness components were assessed using the PREFIT battery. Preschoolers whose parents had higher educational levels had lower fatness (P

This study aimed to analyse the association between socioeconomic status (SES) and fatness and fitness in preschoolers. 2,638 preschoolers (3-5 years old; 47.2% girls) participated. SES was estimated from the parental educational and occupational levels, and the marital status. Fatness was assessed by body mass index (BMI), waist circumference (WC), and waist-to-height ratio (WHtR). Physical fitness components were assessed using the PREFIT battery. Preschoolers whose parents had higher educational levels had lower fatness (P 0.05). BMI significantly differed across occupational levels of each parent (P 0.05) and WHtR across paternal levels (P = 0.004). Musculoskeletal fitness was different across any SES factor (P 0.05), except handgrip across paternal occupational levels (P ≥ 0.05). Preschoolers with high paternal occupation had higher speed/agility (P = 0.005), and those with high or low maternal education had higher VO

The physico-chemical and microbiological characteristics of olive oil can be affected by both storage time of the olives before milling, and also the manner in which the oil is stored.The aim of this study was to determine the microbiological profile of olives stored for different periods (7, 15 and 30 days) and to examine the olive oils obtained by measuring changes in physico-chemical and microbiological characteristics, including polyphenol contents and antioxidant capacity, after 3 and 6 months storage.In all olive samples, and also in all the corresponding oils, the main micro-organisms found were mesophilic and psychrotrophic bacteria, yeasts and moulds. Enterobacteriaceae were not detected. It was noted that storage produced a significant increase in acidity, peroxide contents and ultraviolet absorbance, as well caused a significant reduction in polyphenol contents and antioxidant capacity.

The tomato (Lycopersicon esculentum Mill.) is one of the most widely consumed vegetables and is a component of the so-called “Mediterranean diet.” It represents a significant source of antioxidants in the human diet, with an important biological function (lycopene and β-carotene), as well as other components with antioxidant properties. Antioxidant contents differ according to the variety of tomato, and environmental and agronomic conditions of growth. The aim is to optimize an high-performance liquid chromatography method for the determination of bioactive compounds and to use different antioxidant tests to determine free-radical scavenging activity.The analytical parameters, recovery assays higher than 97 %, satisfactory precision with Relative standard deviation values below 8 %, good linearity (r > 0.999), good sensitivity, and appropriate limits of detection and quantification show that the technique used is satisfactory for measuring these compounds. The tomato samples examined contain highly bioactive components and have high antioxidant capacity with high correlation between phenolic compound contents and antioxidant activity.

Analysis of phenolic profile and tocopherol fractions in conjunction with chemometrics techniques were used for the accurate characterization of extra virgin argan oil and eight other edible vegetable virgin oils (olive, soybean, wheat germ, walnut, almond, sesame, avocado, and linseed) and to establish similarities among them. Phenolic profile and tocopherols were determined by HPLC coupled with diode-array and fluorescence detectors, respectively. Multivariate factor analysis (MFA) and linear correlations were applied. Significant negative correlations were found between tocopherols and some of the polyphenols identified, but more intensely (P < 0.001) between the γ-tocopherol and oleuropein, pinoresinol, and luteolin. MFA revealed that tocopherols, especially γ-fraction, most strongly influenced the oil characterization. Among the phenolic compounds, syringic acid, dihydroxybenzoic acid, oleuropein, pinoresinol, and luteolin also contributed to the discrimination of the oils. According to the variables analyzed in the present study, argan oil presented the greatest similarity with walnut oil, followed by sesame and linseed oils. Olive, avocado, and almond oils showed close similarities.

Rum is a distilled alcoholic beverage obtained from sugarcane juice or molasses, with an alcohol content of 35–54%V V − 1 . The manufacturing process involves several stages, including harvesting of the sugary raw material, fermentation, distillation, and aging in oak barrels, the end result being a liquid ranging from amber to mahogany in color, with a particular bouquet of long historical acceptance. Rum-producing areas are almost all in the Americas, Southern Europe, and the Canary Islands, with a small component in the Philippines. This article provides extensive reference to the characteristics of rum, processing techniques, and production areas.

Storage conditions can affect the stability of extra virgin olive oil, with adverse results on quality. The aim of this study is to examine changes in the chemical composition and sensory characteristics of the oil resulting from prolonged storage at different temperatures and depending on the type of container. The influence on the quality of the oil was also considered, based on the analysis of quality parameters, the total phenol contents, changes in minority compounds, the variation of the trolox equivalent antioxidant capacity values (TEAC) and sensory features. At both room temperature (RT) and refrigerated temperature, all container types had the same surface area of exposure to light and air. All the oils stored showed losses in both qualitative properties and minor components, as well as antioxidant capacity and sensory features. This was especially true for oils stored in polyethylene terephthalate and glass, but less so for those stored in Tetra-Brik®.

This paper presents an investigation into the influence of several culinary factors, such as water temperature, infusion time, stirring and dosage form, on polyphenol content and antioxidant capacity (TEAC values) during the domestic preparation of green tea. The results obtained show that water temperature and infusion time strongly influence total polyphenol levels and the antioxidant capacity of green tea. Temperatures of 70–80 °C together with infusion times of 3–5 min produced greatest effect, in this respect. At 90 °C, extraction was faster and more effective. However, prolonged infusion at this temperature may cause a loss of polyphenol compounds and, consequently, of antioxidant capacity. Factors such as agitation and dosage form do not seem to have much influence. Furthermore, it was found that pure green tea infusions have higher antioxidant properties than do blends of green tea with aromatic herbs and fruits.

Various chromatographic techniques were used to identify the β-carotene, squalene and diverse waxes present in extra virgin olive oil obtained by a new cold extraction system. To determine the wax content, solid-phase extraction on a silica-gel column was used and the fraction was analyzed by capillary gas chromatography. The other compounds present in the non-saponifiable olive oil fraction, i.e. squalene and β-carotene, were analyzed by gas chromatography and reversed-phase high-performance liquid chromatography, respectively. An internal standard was used for the waxes, and the squalene and β-carotene were quantified by means of external standard calibration with reference substances. The analytical parameters recorded recovery assays of over 97% and the satisfactory precision achieved (with RSD values below 8%), the good linearity ( r > 0.999), good sensitivity and appropriate limits of detection and quantification, all show that the techniques used are satisfactory for measuring these compounds in extra virgin olive oil. The results obtained indicate that the oils analyzed, obtained by a new cold extraction system, present a higher concentration of components directly associated with the desirable characteristics of extra virgin olive oils.

Potato, tomato, eggplant and pumpkin were deep fried, sauteed and boiled in Mediterranean extra virgin olive oil (EVOO), water, and a water/oil mixture (W/O). We determined the contents of fat, moisture, total phenols (TPC) and eighteen phenolic compounds, as well as antioxidant capacity in the raw vegetables and compared these with contents measured after cooking. Deep frying and sauteing led to increased fat contents and TPC, whereas both types of boiling (in water and W/O) reduced the same. The presence of EVOO in cooking increased the phenolics identified in the raw foods as oleuropein, pinoresinol, hydroxytyrosol and tyrosol, and the contents of vegetable phenolics such as chlorogenic acid and rutin. All the cooking methods conserved or increased the antioxidant capacity measured by DPPH, FRAP and ABTS. Multivariate analyses showed that each cooked vegetable developed specific phenolic and antioxidant activity profiles resulting from the characteristics of the raw vegetables and the cooking techniques.

Publisher Summary Many methods to measure antioxidant capacity (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) diammonium salt; 1,1-diphenyl-2-picrylhydrazyl) show a good response in vitro but a poor response in vivo. Diets that contain foods with significant quantities of antioxidants can increase the antioxidant capacity of plasma, adding to the effect of endogenous antioxidants. Antioxidants in biological samples are unstable, especially when exposed to air or light at room temperature. Antioxidant capacity (AC) has been determined in plasma by some authors and in serum by others, finding no differences in values between media in some cases and higher values in serum in others. This latter result is unexpected, since serum is obtained by a process of coagulation at room temperature (22°C) for 30 min. Both factors (time and temperature) can interfere with antioxidants such as ascorbic acid and glutathione, which can lower the AC value measured in the serum. Therefore, plasma is used to measure AC to avoid the reactive species generated in the coagulation process, which can compromise the AC measurement. It is recommended that plasma be centrifuged under refrigeration temperatures to minimize thermal stress to antioxidants.