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Among toxic elements, arsenic (As) comes under group 1 carcinogenic metalloid. Its presence in the soil and irrigation water in a higher concentration than permissible limit has become a threat to crop production and human livelihood. Crop plants, specifically those used as staple foods, exhibit the highest As accumulation which subsequently accumulates in the human body after their consumption, leading to severe fatal diseases. As occurs in two main inorganic forms including trivalent (As(III)) and pentavalent (As(V)), of which the trivalent form is more toxic. In plants, uptake of As(III) is affected by oxidizing or reducing environment of the soil and the pH and is mediated by various transporters such as Nodulin-26-like aquaporins (such as Lsi1 and Lsi2). Plants utilize various strategies that help them to withstand the toxic effect of As(III) including reshuffling in many biochemical and physiological processes. These strategies include the use of endogenously generated or exogenously applied chemicals or plant growth regulators. This review article discusses the uptake, transport, and various mechanisms to tolerate higher As(III) in plants. Besides, an update on the use of protectants in curtailing As(III) toxicity in crop plants has also been described., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

The escalating impact of abiotic stress on crop productivity requires innovative strategies to ensure sustainable agriculture. This review examines the promising role of biostimulants in mitigating the adverse effects of abiotic stress on crops. Biostimulants, ranging from simple organic compounds to complex living microorganisms, have demonstrated significant potential in enhancing plant resilience, stress tolerance, and overall performance. The mechanisms underlying biostimulant action-such as enhancing antioxidant defenses, regulating hormonal pathways, and inducing metabolic adjustments-are reviewed. Furthermore, we incorporate the latest research findings, methodologies, and advancements in biostimulant applications for addressing abiotic stressors, including drought, salinity, high temperatures, and nutrient deficiencies. This review also highlights current challenges and future opportunities for optimizing biostimulant use in sustainable crop production. This revision aims to guide researchers and agronomists in applying biostimulants to improve crop resilience in the context of climate change.

Pesticides are essential in contemporary agriculture, as they improve crop yields and quality while safeguarding against pests. However, long-term heavy use of traditional pesticides has led to increased pest resistance, while these pesticides are often toxic and less selective, and may also have adverse effects on the environment and nontarget organisms. To solve this problem, it is important to find new targets for pesticide to develop more effective and environmentally friendly alternatives. Therefore, exploring new pesticide action targets has become one of the current research focuses. In the past years, efforts have been made to investigate possible strategies, and this work systematically summarizes and criticizes the most recently used ones. This contribution not only helps promote the research and development of new pesticides but also holds substantial implications for promoting the sustainable development of agriculture and food security.

Ozone (O 3 ), a major air pollutant, can negatively impact plant growth and yield. While O 3 impacts have been widely documented in crops such as wheat and soybean, few studies have looked at the effects of O 3 on sorghum, a C 4 plant and the fifth most important cereal crop worldwide. We exposed grain sorghum ( Sorghum bicolor cv. HAT150843) to a range of O 3 concentrations (daytime mean O 3 concentrations ranged between 20 and 97 ppb) in open-top chambers, and examined how whole plant and leaf morphological traits varied in response to O 3 exposure. Results showed no significant impact of realistic O 3 exposure on whole plant biomass and its partitioning in sorghum. These findings suggest that sorghum is generally resistant to O 3 and should be considered as a favourable crop in O 3 polluted regions, while acknowledging further research is needed to understand the mechanistic basis of O 3 tolerance in sorghum., Competing Interests: The authors declare that they have no competing interests., (© 2025 Farha et al.)

Nanoenabled agrochemicals mainly including nanopesticides and nanofertilizers based on nanotechnology play a crucial role in plant protection and food security. These agrochemicals exhibit high dose delivery efficiency and biological activity due to their unique nanoscale properties. However, nanoscale properties can also be a double-edged sword, posing potential risks to both humans and the environment. As nanoenabled agrochemicals become more widely used, it is essential to have an objective and comprehensive discussion of the size effects of these agrochemicals. In this paper, we reviewed the research progress on the size effects of nanoenabled agrochemicals in terms of dose delivery, biological activity, and nontarget safety. We investigated the complex factors affecting size effects and sought to draw insights from research in biomedicine, engineering, food, and other relevant fields. Based on the literatures review, it could be concluded that "the smaller the better" is not always the case. We further outlooked the development prospects of studying the size effects of nanoenabled agrochemicals, emphasizing the necessity for thorough and in-depth research while critically identifying key issues that need to be addressed. In conclusion, a proper comprehension of the size effects of nanoenabled agrochemicals bridges the gap between the scientific community and industry, bolstering the role in advancing sustainable agriculture.

Fertilization with animal manure and sewage sludge, and the use of sewage water for irrigation, can lead to high antimicrobial concentrations in agricultural soils. Once in soil, antimicrobials can exert direct and indirect toxic effects on plants by misbalancing plant-microbe symbiotic relationships. We performed germination tests to determine the optimum germination conditions of 24 plant species (10 crop and 14 wild species). Subsequently, we analyzed the differences in oxytetracycline and sulfamethazine phytotoxicity in 19 plant species for which optimum germination conditions could be established. The root elongation of the majority of wild species was inhibited in the presence of oxytetracycline and sulfamethazine, whereas crops were mainly affected by oxytetracycline. There were no differences in sensitivity to oxytetracycline between crop and wild plant species, whereas wild plants were significantly more susceptible to sulfamethazine than crop species. Thus, to cover both productivity and biodiversity protection goals, we recommend pharmaceuticals' predicted no-effect concentration (PNEC) values based on crop and wild plant species phytotoxicity data., Competing Interests: Declarations. Conflict of interests: The authors declare that they have no competing interests., (© 2024. The Author(s).)

Pesticide pollution has emerged as a critical global environmental issue of pervasive concern. Although the application of pesticides has provided substantial benefits in controlling weeds, pests, and crop diseases, their indiscriminate use poses considerable challenges to soil health and food safety. Pesticides can be absorbed by crops through either foliar or root uptake, resulting in deleterious effects such as extensive tissue damage, growth inhibition, and reduced crop quality. Beside these visible effects, pesticides can alter gene expression and disrupt cellular signaling transduction, thereby interfering with essential metabolic processes even inducing toxic stress. Moreover, pesticides can interact intricately with biomolecules (e.g. proteins, nucleic acid) in crops, causing significant alterations in protein structure and physiological function. This review focuses on pesticide residues and their associated toxicity, emphasizing their pervasive influence on vital physiological and metabolic pathways, including carbohydrate metabolism, amino acid metabolism, and fatty acid metabolism. Particular attention is given to elucidating the molecular mechanisms underlying these disturbances, specifically regarding transcriptional regulation, cell signaling pathways, and biomolecular interactions. This review provides a comprehensive understanding of multifaceted effects of pesticides and to underscore the necessity for sustainable agricultural practices to safeguard crop yield and quality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

To investigate the effects of modified carbon-based materials on soil environmental remediation and crop physiological regulation, this research relied on rice pots with lead (Pb) and cadmium (Cd) composite contamination. Dolomite, montmorillonite, attapulgite and sepiolite modified biochar with different doses have been developed to explore the mechanisms on heavy metal passivation, nutrient improvement, microbial activation, and crop growth. The results indicated that the modified materials effectively reduced heavy metal bioavailability and accumulation in plant tissues through adsorption complexation. Specifically, under montmorillonite and sepiolite modified treatments, the Grains-Pb content significantly decreased by 29.23-30.31% and 27.49-30.58%, compared to the control group (CK). Meantime, carbon-based materials increased available nutrient levels, providing a biological substrate for soil microorganisms metabolism. The content of ammonium nitrogen (NH 4 + -N) and available phosphorus (AP) in different proportions of montmorillonite modified biochar increased by 10.99-13.98% and 55.76-77.86%, respectively, compared to CK. Furthermore, sepiolite modified biochar enhanced bacterial community diversity, significantly improving the tolerance and resistance of bacterial communities such as Proteobacteria and Acidobacteria to heavy metals. Meanwhile, carbon-based materials enhanced community stability and network complexity, improving microbial stress resistance to adverse environments. In summary, montmorillonite and sepiolite modified biochar regulated microbial community interaction mechanisms by mitigating the physiological toxicity of heavy metals. This process enhanced soil available nutrients and ecological function stability, which had significant implications for improving crop growth and quality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

While diuron residues are being detected more frequently in agricultural soils, there is limited information available regarding their potential phytotoxicity to non-target grain crops. This study aims to determine robust phytotoxicity thresholds for three common, but contrasting, crop species (canola, chickpea, and wheat) exposed to a range of diuron concentrations and to determine how loamy sand soil can change the toxicity thresholds relative to an inert sand. The log-logistic non-linear regression model proved most effective in determining toxicity thresholds by analysing crop responses to diuron. Canola was the most sensitive to diuron in sand followed by wheat and chickpea. Diuron exhibits higher phytotoxicity in sand compared to loamy sand, with ED50 values (which is the dose at which diuron causes a 50% decrease in plant growth) of 0.03 mg kg-1 and 0.07 mg kg-1 for canola shoot biomass inhibition and 0.01 mg kg-1 and 0.06 mg kg-1 for root dry weight reduction, respectively. The ED50 values for wheat shoot biomass (0.11 and 0.24 mg kg-1) in sand and loamy sand, respectively, and the ED50 values for root growth inhibition are 0.14 mg kg-1 in sand and 0.19 mg kg-1 in loamy sand. These values were lower than label concentrations and previously estimated average and maximum diuron residue loads (0.17 and 0.29 mg kg-1) in Western Australia paddocks. The larger ED50 values of diuron in the loamy sand can be attributed to higher soil organic matter and cation exchange capacity that decreased bio-available diuron levels. Average diuron residue loads in Western Australia crop fields exceed the ED50 value emphasizes the need for careful planning of crop rotations to avoid crop phytotoxicity from soil-borne diuron residues. Further study is needed to determine the effect of a wider range of soil properties such as pH, clay content, and soil organic matter on the phytotoxicity risk of diuron to rotational crops., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Pyone et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Soil contamination by cadmium (Cd) is an increasing environmental concern that potentially jeopardizes both crop productivity and human health. Silicon (Si), the Earth's second most abundant element, has shown a significant potential in reducing Cd uptake by crops. However, there is still a lack of quantitative data on the beneficial effects of Si in reducing Cd toxicity, thereby making it more difficult to ensure safe crop production. We conducted a comprehensive meta-analysis of 105 studies to assess the impact of exogenous Si on Cd accumulation in three major cereal crops (wheat, maize, and rice) and elucidate the key factors governing the Si effects. We found that Si supplementation significantly mitigated Cd toxicity in crops, reducing Cd accumulation in maize, rice, and wheat by 37 %, 30 %, and 45 %, respectively. This reduction was most pronounced in all three crop grains (reductions reaching 40-51 %). The four different forms of Si applied all increased crop yield, with nano-silicon resulting in an average yield increase of 19 %, surpassing silicate, Si-based fertilizer, and other silica-based materials. The effects of Si were primarily influenced by application rate and methods, soil pH, Cd concentration, and the effects of foliar and field application. Based on Cd inhibition levels and overall economic benefits, we recommend a Si application rate of ≤ 250 mg/kg, preferably using nano-silicon or silicate. Overall, our study provides valuable globally relevant guidance on Si amendments selection and application thereby ensuring safer and higher crop production in Cd-contaminated soils., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The increasing abiotic stresses from changing global climatic conditions, including drought, extreme temperatures, salinity, storms, pollutants, and floods, impend crop cultivation and sustainability. To mitigate these effects, numerous synthetic and non-synthetic chemicals or plant growth regulators are in practice. Chitosan, a natural organic substance rich in nitrogen and carbon, and thiourea, a synthetic plant growth regulator containing sulfur and nitrogen, have garnered significant interest for their roles in enhancing plant stress tolerance. Despite extensive use, the precise mechanisms of their actions remain unclear. Towards this endeavor, the present review examines how chitosan and thiourea contribute to stress tolerance in crop plants, particularly under drought conditions, to improve production and sustainability. It also explores thiourea's potential as a hydrogen sulfide (H 2 S) donor and the possible applications of thiolated chitosan derivatives and chitosan-thiourea combinations, emphasizing their biological functions and benefits for sustainable agriculture., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier GmbH. All rights reserved.)

Contamination of agricultural soils with heavy metal(loid)s like arsenic (As) and cadmium (Cd) is an ever increasing concern for crop production, quality, and global food security. Numerous in-situ and ex-situ remediation approaches have been developed to reduce As and Cd contamination in soils. However, field-scale applications of conventional remediation techniques are limited due to the associated environmental risks, low efficacy, and large capital investments. Recently, calcium (Ca) and Ca-based nano-formulations have emerged as promising solutions with the large potential to mitigate As and Cd toxicity in soil for plants. This review provides comprehensive insights into the phytotoxic effects of As and Cd stress/toxicity and discusses the applications of Ca-based ionic and nano-agrochemicals to alleviate As and Cd toxicity in important crops such as rice, wheat, maize, and barley. Further, various molecular and physiological mechanisms induced by ionic and nano Ca to mitigate As and Cd stress/toxicity in plants are discussed. This review also critically analyzes the efficiency of these emerging Ca-based approaches, both ionic and nano-formulations, in mitigating As and Cd toxicity in comparison to conventional remediation techniques. Additionally, future perspectives and ecological concerns of the remediation approaches encompassing ionic and nano Ca have been discussed. Overall, the review provides an updated and in-depth knowledge for developing sustainable and effective strategies to address the challenges posed by As and Cd contamination in agricultural crops., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Non-steroidal anti-inflammatory drugs, diclofenac (DCF) and naproxen (NPX), represent a group of environmental contaminants often detected in various water and soil samples. This work aimed to assess possible phytotoxic effects of DCF and NPX in concentrations 0.1, 1 and 10 mg/L, both individually and in binary mixtures, on the seed germination and primary root elongation of crops, monocots Allium porrum and Zea mays, and dicots Lactuca sativa and Pisum sativum. Results proved that the seed germination was affected by neither individual drugs nor their mixture. The response of primary root length in monocot and dicot species to the same treatment was different. The Inhibition index (%) comparing the root length of drug-treated plants to controls proved to be approximately 10% inhibition in the case of dicots lettuce and pea, and nearly 20% inhibition in monocot leek, but almost 20% stimulation in monocot maize. Assessment of the binary mixture effect confirmed neither synergistic nor antagonistic interaction of DCF and NPX on early plant development in the applied concentration range., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Sustainable agriculture has become prime importance to feed growing population. To achieve this goal application of exogenous hormones and signaling molecules are gaining important. In this context, we have investigated potential of ethylene (25 μM ethephon; donor) and H 2 S (10 μM NaHS; donor) in mitigating hexavalent chromium [Cr (VI), 50 μM] toxicity in two pulse seedlings: black bean and mung bean. Cr(VI) declined growth and gas exchange parameters (photosynthetic rate, stomatal conductance, sub cellular CO 2 concentration, and transpiration level) which was accompanied by intracellular accumulation of Cr in both pulse crops and the damaging effect was greater in mung bean seedlings. The suppression in the growth and related parameters was occurred due to higher buildup of oxidative stress markers; O 2 • ‾, H 2 O 2 , lipid peroxidation (as malondialdehyde, MDA equivalents) and membrane injury in leaf and root of both pulse crops. Cr induced disturbance in AsA-GSH cycle (reduction in the activity of glutathione reductase, ascorbate peroxidase, monodehydroascorbate reductase and dehydroascorbate reductase, and the amount of ASA and GSH) could be one of the reasons for greater accumulation of H 2 O 2 . Further, exogenous application of ethylene and H 2 S significantly ameliorated Cr toxicity on growth and photosynthetic activity by significantly lowering the intracellular Cr accumulation and oxidative biomarkers, and also by strengthening the activity of AsA-GSH cycle. The exogenous application of biosynthesis inhibitors of ethylene (AVG) and H 2 S (PAG) caused greater damaging effect on these parameters due to more accumulation of Cr(VI), thereby suggesting that the endogenous levels of these regulators are critical for Cr(VI) tolerance. Interestingly, ET did not rescue adverse effects of Cr(VI) in absence of endogenous H 2 S, while H 2 S could do so even without endogenous ethylene, suggesting that H 2 S played downstream signaling to ethylene in regulating Cr(VI) toxicity. Hence, being cheap and easily available theses growth regulators may be considered for sustainable agriculture., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Carbon Dots (CDs) were synthesized from peanut shells (PNS) through pyrolysis and characterized using FTIR, XRD, HRTEM and BET analysis revealing an average size of 2-5 nm with amorphous nature. Synthesized PNS-CDs was employed both as priming and foliar agent for enhancing seed quality and crop productivity in blackgram (Vigna mungo L. Hepper). Different concentrations ranging from 50, 100, 200, 300, 400, 500 and 1000 ppm was used for seed priming and 5, 10, 15, 20, 25, 50, 75 and 100 ppm were given as foliar spray on 30 th and 45 th days after sowing (DAS). On accounting the best seed priming and foliar spray concentrations, field trial was conducted to validate the optimistic effect of PNS-CDs on blackgram crop productivity. Results revealed that priming with 200 ppm for 3 h exhibited maximum seed imbibition (54%), germination (88%) and vigour index (3165). Whereas, foliar spray with 50 ppm expressed significant improvement in leaf area index (2.6), total chlorophyll (2.70 mg/g), total soluble protein (71 mg/g), Number of nodules/plant (138), seed yield/plant (8.7 g) and 100 seed weight (5 g). The impact of PNS-CDs treatments resulted in increased photosynthetic rate (12.45 µmol CO 2 m -2 s -1 ), transpiration rate (3.13 mmol H 2 O/m -2 s -1 ), stomatal conductance (0.55 mol H 2 O/m -2 s -1 ) and internal leaf CO 2 concentration (652 µmol CO 2 m -2 s -1 ) which ultimately enhanced the photosynthetic efficiency of plants. It has also exhibited a promising effect on the resultant seed in which the combination seed priming (200 ppm) followed by foliar spray (50 ppm) recorded maximum 100 seed weight (4.19 g), germination (97%) and vigour index (3019). Thus, this study highlights the promising role of PNS-CDs as a sustainable and effective agricultural nanomaterial, offering a novel approach to utilize the agricultural waste and also to enhance the crop productivity through advanced non-chemical approach., (© 2024. The Author(s).)

Pesticides are extensively utilized in contemporary agriculture to manage pests, enhance crop yields, and sustain productivity. Nevertheless, the persistent herbicide represents a dual-edged weapon. On one hand, their prolonged efficacy enables reduced application frequency during crop growth seasons, resulting in cost savings on labor. However, the presence of these residues within fields poses safety risks to soil quality, sensitive crops in subsequent rotations, agricultural product quality, and the ecological environment. This review presents a comprehensive review on the mechanisms of action, application risks, ecotoxicology, and residue analysis methods of nine representative persistent herbicides (namely, atrazine, imazethapyr, imazapic, mesosulfuron-methyl, halosulfuron-methyl, fomesafen, diflufenican, quinclorac, and pyroxasulfone). The objective is to guide their scientific and rational utilization in agricultural practices while minimizing phytotoxicity risks and effectively monitoring and controlling soil pollution. These can not only provide practical recommendations for mitigating potential plant toxicity and ecological environmental risks but also contribute valuable technical insights for efficient soil pollution monitoring and prevention. Additionally, unaddressed research objectives were also anticipated.

Human existence and the long-term viability of society depend on agriculture. Overuse of synthetic fertilizers results in increased contamination of the land, water, and atmosphere as well as financial constraints. In today's modern agriculture, environmentally friendly technology is becoming more and more significant as a substitute for conventional fertilizers and chemical pesticides. Using nanotechnology, agricultural output can be improved in terms of quality, biological support, financial stability, and environmental safety. There is a lot of promise for the sustainable application of nano-fertilizers in crop productivity and soil fertility, with little or no negative environmental effects. In this context, the present review provided an overview of the benefits of using nanofertilizers, its application and types. Mechanistic approach for increasing soil fertility and yield via nanofertilizers also described in detail. We concluded this article to compare the advantages of nanofertilizers over chemicals and nano-chemicals. Nonetheless, additional investigation is required to comprehend the effects and possible hazards of nanomaterials in the food production chain., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier GmbH. All rights reserved.)

Key Message: Boron is essential for plants, but excess can induce toxicity. Boron (B) is a vital micronutrient for plants, but excess B can induce toxicity symptoms and reduce crop yields. B bioavailability depends on soil properties, including clay type, pH, and organic matter content. Symptoms of B toxicity include reduced shoot and root growth, leaf chlorosis and necrosis, impaired photosynthesis, and disrupted pollen development. This review paper examines the current knowledge on B toxicity mechanisms, tolerance strategies, and management approaches in plants. This review covers (1) factors affecting B bioavailability; (2) toxicity symptoms in plants; (3) uptake, transport, and detoxification mechanisms; and (4) strategies. To mitigate toxicity, plants reduce B uptake, activate efflux transporters, compartmentalize B, and enhance antioxidant systems. On the basis of this review, future research should focus on identifying novel tolerance mechanisms, exploring genetic strategies for improved B management, and developing innovative agronomic interventions. These insights will facilitate the breeding and management of crops for enhanced productivity under B toxicity stress., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The utilization of carbon dots (CDs) in agriculture to enhance plant growth has gained significant attention, but the data remains fractionated. Systematically integrating existing data is needed to identify the factors driving the interactions between CDs and plants and strategically guide future research. Articles reporting on CDs and their effects on plants were searched based on inclusion and exclusion criteria, resulting in the collection of 71 articles comprising a total of 2564 data points. The meta-analysis reveals that the soil and foliar application of red-emitting bio-derived CDs at a low concentration (<10 ppm) leads to the most beneficial effects on plant growth. Random forest and gradient boosting algorithms revealed that the size and dose of CDs were important factors in predicting plant responses across multiple aspects (CDs properties, plant properties, environmental factors, and experimental conditions). Specifically, smaller sizes are more favorable to growth indicators (GI) below 6 nm, nutrient and quality (NuQ) at 3-6 nm, photosynthesis (PSN) below 7 nm, and antioxidant responses (AR) below 5 nm. Overall, our analysis of existing data suggests that CDs applications can significantly improve plant responses (GI, NuQ, PSN, and AR) by 10-39 %. To unlock the full potential of CDs, customized synthesis techniques should be employed to meet the specific requirements of different crops and climate condition. For example, we recommend the synthesis of small CDs (<7 nm) with emission peak values falling within the range of 405-475 and 610-670 nm to enhance plant growth. The global prediction of plant responses to CDs application in future scenarios have shown significant improvements ranging from 17 to 58 %, suggesting that CDs have widespread applicability. This novel understanding of the impact of CDs on plant response provides valuable insights for optimizing the application of these nanomaterials in agriculture., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The utilisation of coated controlled-release fertilizers (CRFs) leads to the persistence of residual plastic films in agricultural soils, posing a potential threat to crop health. This study investigates the impacts of four residual films (0.39 %, w/w) derived from CRFs in soil, including petrochemical polyether, bio-based polyether, castor oil polyester, and wheat straw polyester polyurethane on wheat growth. This study found that PecPEUR significantly reduced wheat plant height, stem diameter, leaf area, and aboveground fresh weight by 24.8 %, 20.2 %, and 25.7 %. Through an in-depth exploration of transcriptomics and metabolomics, it has been discovered that all residual films disrupted glycolysis-related metabolic pathways in wheat roots, affecting seedling growth. Among them, PecPEUR significantly reduced the fresh weight of aboveground parts by 20.5 %. In contrast, polyester polyurethane residue had no discernible impact on aboveground wheat growth. This was attributed to the enrichment of wheat root genes in jasmonic acid and γ-aminobutyric acid metabolic pathways, thus mitigating oxidative stress, enhancing stress resistance, and ensuring normal plant growth. This study, for the first time, provides comprehensive insights into the effects of polyurethane film residue on wheat seedling growth, underscoring its potential as a promising alternative to conventional plastics in soil., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

The relative severity between chromium (Cr)-mediated ecotoxicity and its bioaccumulation has rarely been compared and evaluated. This study employed pot incubation experiments to simulate the soil environment with increased Cr pollution and study their effects on the growth of crops, including pepper, lettuce, wheat, and rice. Results showed that increasing total Cr presented ascendant ecotoxicity in upland soils when pH > 7.5, and significantly reduced the yield of pepper, lettuce and wheat grain by 0.3-100 %, whereas, this effect was weakened even reversed as the pH decreased. Surprisingly, a series of soils with Cr concentration of 22.7-623.5 mg kg -1 did not cause Cr accumulation in four crops over the Chinese permissible limit. The toxicity of Cr was highly associated with extractable Cr, where Cr (VI) made the greater contributions than Cr (III). Conclusively, the ecotoxicity of Cr poses a greater environmental issue as compared to the bioaccumulation of Cr in crops in upland soils, while extractable Cr (VI) makes the predominant contributions to the ecotoxicity of Cr as the total Cr increased. Our study proposes a synchronous consideration involving total Cr and Cr (VI) as the theoretical basis to establish a more reliable soil quality standard for safe production in China., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The present study was designed to assess the allelopathic potential of invasive weed Ageratina adenophora leaf extracts on seed germination and seedling development efficiency of native tree [viz. Quercus leucotrichophora A. Camus (Oak) and Pinus roxburghii Sarg. (Pine)] and crop [(Triticum aestivum L. (Wheat) and Lens culinaris Medik. (Lentil)] species of Kumaun Himalaya. Pot experiments were conducted in the glasshouse of the Botany Department, D.S.B. Campus, Kumaun University Nainital, following a Completely Randomized Block Design (CRBD) with three treatments (C 1 -25%, C 2 -50%, and C 3 -100% of aqueous leaf extract) and one control, each with five replicates. The experiment lasted one year for tree species and continued until the seed maturation phase for crop species. Parameters such as seed germination proportion, root and shoot measurements, biomass, and crop productivity traits were recorded accordingly. Our bioassay results indicated that the inhibitory effect of leaf extracts on the measured traits of the selected native species was proportional to the applied extract concentrations of A. adenophora. Overall, lentil among crops and oak among tree species exhibited more inhibition compared to wheat and pine, respectively. At the highest concentration, reductions of 44%, 34%, 36%, and 24% in biomass production capacity were recorded for wheat, lentil, pine, and oak, respectively, while wheat and lentil productivity decreased by up to 33% and 45%, respectively. These results suggest that water-soluble allelochemicals produced by A. adenophora may impede the establishment of selected crop and tree species in agroecosystems and forest ecosystems invaded by this weed species. However, further studies on the characterization of phytochemicals and their specific role in seed germination and growth are warranted. Furthermore, the allelopathic potential of A. adenophora can be explored for the preparation of biopesticides and nature-friendly option to improve soil health, crop productivity, and reduce environmental pollution and management of this invasive weed., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Organic fertilization is a major driver potentiating soil antibiotic resistance in farmland. However, it remains unclear how bacterial antibiotic resistance evolves in fertilized soils and even spreads to crops. Compared with no fertilizer and commercial fertilizer treatments, organic fertilizers markedly increased the abundance of soil antibiotic resistance genes (ARGs) but the relatively weaker transfer of resistance genes from soil to crops. The introduction of organic fertilizers enriches the soil with nutrients, driving indigenous microorganisms towards a K-strategy. The pH, EC, and nutrients as key drivers influenced the ARGs abundance. The neutral (pH 7.2), low salt (TDS 1.4 %) and mesotrophic (carbon content 3.54 g/L) habitats similar to the soil environment conditioned by organic fertilizers. These environmental conditions clearly prolonged the persistence of resistant plasmids, and facilitated their dissemination to massive conjugators soil microbiome but not to plant endophytes. This suggested that organic fertilizers inhibited the spread of ARGs to crops. Moreover, the composition of conjugators showed differential selection of resistant plasmids by endophytes under these conditions. This study sheds light on the evolution and dissemination of antibiotic resistance in farmlands and can aid in the development of antimicrobial resistance control strategies in agriculture., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Arsenic (As) is an acute toxic metalloid that affects plant growth and development. As is found in the environment in organic and inorganic forms, but arsenite As(III) and arsenate As(V) are the most prevalent forms that negatively impact the plants. Roots exposed to As can easily absorb it mainly through transporters that carry vital mineral nutrients. As reach the food chain via crops irrigated with As-polluted water and exerts a negative impact. Even at low levels, As exposure disrupts the regular functioning of plants by generating a high level of reactive oxygen species (ROS) results into oxidative damage, and disruption of redox system. Plants have built-in defence mechanisms to combat this oxidative damage. The development of a food crop with lower As levels is dependent upon understanding the molecular process of As detoxification in plants, which will help reduce the consumption of As-contaminated food. Numerous genes in plants that may provide tolerance under hazardous conditions have been examined using genetic engineering techniques. The suppression of genes by RNA interference (RNAi) and CRISPR-Cas 9 (CRISPR associated protein 9) technology revealed an intriguing approach for developing a crop that has minimal As levels in consumable portions. This study aims to present current information on the biochemical and molecular networks associated with As uptake, as well as recent advances in the field of As mitigation using exogenous salicylic acid (SA), Serendipita indica and biotechnological tools in terms of generating As-tolerant plants with low As accumulation., Competing Interests: Declaration of competing interest Authors declare no Conflicts of interest/Competing interests., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Implementing sustainable crop protection practices is crucial to protect global harvests and ensure high-quality food supplies. While priming is an established method in seed production for the fortification of plants against various stresses, it is not yet a standard practice in transplant cultivation. Thus, we evaluated the long-term effects of thermopriming-a heat-based priming technique-on the growth, development, and fruit yield of tomato plants. Following a recovery period of about six weeks for thermoprimed plants without stress inducers, we subjected them to subsequent salt stress to ascertain the persistence of the priming effects. Additionally, we compared the efficacy of thermopriming with benzothiadiazole (BTH), a chemical elicitor, in enhancing plant resilience to abiotic stress. While BTH application negatively impacted both plant growth and fruit health, thermopriming showed no such adverse effects on these parameters. Instead, thermopriming initially enhanced the plant defense mechanisms by increasing the accumulation of protective phenols and flavonoids in the leaves. Interestingly, while thermopriming did not alter the response to salt stress, it notably strengthened the overall resilience of the plants. Our findings underscore both the potential and temporal constraints of thermopriming memory. Nonetheless, primed plants exhibited temporarily increased stress tolerance, offering a means to safeguard the offspring.

By 2050, the global population is projected to reach 9 billion, underscoring the imperative for innovative solutions to increase grain yield and enhance food security. Nanotechnology has emerged as a powerful tool, providing unique solutions to this challenge. Nanoparticles (NPs) can improve plant growth and nutrition under normal conditions through their high surface-to-volume ratio and unique physical and chemical properties. Moreover, they can be used to monitor crop health status and augment plant resilience against abiotic stresses (such as salinity, drought, heavy metals, and extreme temperatures) that endanger global agriculture. Application of NPs can enhance stress tolerance mechanisms in plants, minimizing potential yield losses and underscoring the potential of NPs to raise crop yield and quality. This review highlights the need for a comprehensive exploration of the environmental implications and safety of nanomaterials and provides valuable guidelines for researchers, policymakers, and agricultural practitioners. With thoughtful stewardship, nanotechnology holds immense promise in shaping environmentally sustainable agriculture amid escalating environmental challenges., (© 2024 Institute of Botany, Chinese Academy of Sciences.)

Nanomaterials (NMs) have proven to be a game-changer in agriculture, showcasing their potential to boost plant growth and safeguarding crops. The agricultural sector has widely adopted NMs, benefiting from their small size, high surface area, and optical properties to augment crop productivity and provide protection against various stressors. This is attributed to their unique characteristics, contributing to their widespread use in agriculture. Human exposure from various components of agro-environmental sectors (soil, crops) NMs residues are likely to upsurge with exposure paths may stimulates bioaccumulation in food chain. With the aim to achieve sustainability, nanotechnology (NTs) do exhibit its potentials in various domains of agriculture also have its flip side too. In this review article we have opted a fusion approach using bibliometric based analysis of global research trend followed by a holistic assessment of pros and cons i.e. toxicological aspect too. Moreover, we have also tried to analyse the current scenario of policy associated with the application of NMs in agro-environment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Stomatal operation is crucial for optimising plant water and gas exchange and represents a major trait conferring abiotic stress tolerance in plants. About 56% of agricultural land around the globe is classified as acidic, and Al toxicity is a major limiting factor affecting plant performance in such soils. While most of the research work in the field discusses the impact of major abiotic stresses such as drought or salinity on stomatal operation, the impact of toxic metals and, specifically aluminium (Al) on stomatal operation receives much less attention. We aim to fill this knowledge gap by summarizing the current knowledge of the adverse effects of acid soils on plant stomatal development and operation. We summarised the knowledge of stomatal responses to both long-term and transient Al exposure, explored molecular mechanisms underlying plant adaptations to Al toxicity, and elucidated regulatory networks that alleviate Al toxicity. It is shown that Al-induced stomatal closure involves regulations of core stomatal signalling components, such as ROS, NO, and CO 2 and key elements of ABA signalling. We also discuss possible targets and pathway to modify stomatal operation in plants grown in acid soils thus reducing the impact of Al toxicity on plant growth and yield., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)

Chickpea is a highly nutritious protein-rich source and one of the major crops to alleviate global malnutrition, but poor seed quality affects its productivity. Seed quality is essential for better crop establishment and higher yields, particularly in the uncertain climate change. The present study investigated the impact of botanical priming versus hydropriming and bavistin seed treatment on chickpea seeds. A detailed physiological (germination percentage, root and shoot length, vigour index) and biochemical (amylase, protease, dehydrogenase, phytase, and lipid peroxidation) analysis was carried out in order to assess the effect of priming treatments. Turmeric-primed seeds showed better germination rate (94.5%), seedling length, enzyme activity, and lower malondialdehyde (MDA) content. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed the expression of minor polypeptides of albumin and globulin in the primed seeds. Moreover, field experiments indicated increased crop growth, vigour, days to 50% flowering, yield and its attributing traits in turmeric-primed seeds. Botanical priming can increase chickpea yield by up to 16% over the control group. This low-cost and eco-friendly technique enhances seed and crop performance, making it a powerful tool for augmenting chickpea growth. Therefore, chickpea growers must adopt botanical priming techniques to enhance the quality of seed and crop performance. Moreover, this approach is environmentally sustainable and can help conserve natural resources in the long term. Therefore, this new approach must be widely adopted across the agricultural industry to ensure sustainable and profitable farming practices., (© 2024. The Author(s).)

The objective was to evaluate the efficacy of pre-emergence herbicides mixture applied to the soil with and without dead cover crops ( Sorghum bicolor ) for the control of Amaranthus hybridus L. (smooth pigweed) and its selectivity in soybeans. This study was structured in split plot (2 × 6 + 2), where factor A plots (with and without dead cover) and factor B six herbicides mixture: flumioxazin + S-metolachlor (50.4 + 1,008 g a.i. ha -1 ), flumioxazin + imazethapyr (60 + 127.2 g a.i. ha -1 ), pyroxasulfone + sulfentrazone (137.6 + 160 g a.i. ha -1 ), diuron + sulfentrazone (400 + 200 g a.i. ha -1 ), metribuzin + S-metolachlor (326.4 + 1,344 g a.i. ha -1 ) and sulfentrazone + imazethapyr (200 + 100 g a.i. ha -1 ) and two untreated control plots. As for the results, the herbicides flumioxazin + S-metolachlor, flumioxazin + imazethapyr and pyroxasulfone + sulfentrazone showed excellent control (97-99%) and were not influenced by the plot with and without dead cover. They also showed higher yield soybeans (<2,244 kg ha -1 ). All herbicides were selective to the soybeans. Overall, pre-emergence herbicides and cover crops were efficient methods for the control of A. hybridus , which farmers should use to avoid losses in yield soybeans due to weed competition.

Harvested water bamboo shoots can be stored for only a few days before they lose weight and become soft. Nitrogen oxide (NO) and modified atmosphere packaging (MAP) have previously been used to prolong horticultural crop storage. In the present study, we analyzed the joint effect of these two methods on extending the postharvest quality of water bamboo shoots. Water bamboo shoots were treated with (1) 30 μL L -1 NO, (2) MAP, and (3) a combination of NO and MAP. The NO treatment delayed the softness and weight loss through maintaining the integrity of the mitochondrial ultrastructure and enhancing the ATP level by activating the expressions and activities of succinic dehydrogenase, malic acid dehydrogenase, and cytochrome oxidase. MAP improved the effect of NO on the mitochondrial energy metabolism. These results indicate that NO and MAP treatments are effective at suppressing the quality deterioration of water bamboo shoots, MAP improves the effect of NO in extending postharvest life, and NO may be the main effective factor in the combination of NO and MAP.

Synthetic fertilizer and herbicides encompass the largest share in nutrient and weed management on food grain crops that create serious environmental issues. Integrated nutrient and non-chemical weed management approaches may help to reduce the chemical load in the environment, maintaining higher weed control efficiency and yield. A field experiment was conducted for two consecutive monsoon seasons during 2015 and 2016 in farm fields to develop a profitable and sustainable rice production system through integrated nutrient and weed management practices. A varied combination of nutrients either alone or integrated with chemical and non-chemical weed management were tested on transplanted rice in a factorial randomized block design with three replications. The results showed that the integration of concentrated organic manures with chemical fertilizer effectively inhibited weed growth and nutrient removal. Integration of nutrient and weed management practices significantly enhanced 9% biomass growth, 10% yield of the rice crop along with 3-7% higher nutrient uptake. Brassicaceous seed meal (BSM) and neem cake also had some influence on weed suppression and economic return. Thus, the integrated nutrient and weed management practices in rice cultivation might be an effective way to achieve economic sustainability and efficient rice cultivation in eastern India. Shortages of farmyard manure and vermicompost could be supplemented by BSM and neem cake in the integrated module., Competing Interests: The authors have declared that no competing interests exist.

Boron (B) deficiency is a widespread problem in alkaline soils which affects yield and quality of potato but is often ignored by the growers. That's why, we compared the impact of different methods of boron application (foliar spray, fertigation and soil dressing) along with control on boron use efficiency (BUE), quality and yield of potato in alkaline soils. Boron (0.5 kg ha-1) applied as a foliar spray had significantly increased plant height, tuber per plant, tuber volume and enhanced the quality in terms of vitamin C, starch and B content of potato compared to other methods. Moreover, foliar applied B significantly improved B uptake and it use efficiency over other application methods. B concentration in tubers were strongly correlated with vitamin C and starch contents. The application methods were ranked as foliar spray>fertigation>soil dressing in term of their effectiveness towards potato yield and quality improvement. Thus, for optimum production of good quality potato, B should be applied as foliar spray at the rate of 0.5 kg B ha-1 in existing agro-climatic conditions., Competing Interests: The authors have declared that no competing interests exist.

Strigolactone is a newly discovered type of plant hormone that has multiple roles in modulating plant responses to abiotic stress. Herein, we aimed to investigate the effects of exogenous GR24 (a synthetic analogue of strigolactone) on plant growth, photosynthetic characteristics, carbohydrate levels, endogenous strigolactone content and antioxidant metabolism in cucumber seedlings under low light stress. The results showed that the application of 10 μM GR24 can increase the photosynthetic efficiency and plant biomass of low light-stressed cucumber seedlings. GR24 increased the accumulation of carbohydrates and the synthesis of sucrose-related enzyme activities, enhanced antioxidant enzyme activities and antioxidant substance contents, and reduced the levels of H 2 O 2 and MDA in cucumber seedlings under low light stress. These results indicate that exogenous GR24 might alleviate low light stress-induced growth inhibition by regulating the assimilation of carbon and antioxidants and endogenous strigolactone contents, thereby enhancing the tolerance of cucumber seedlings to low light stress., (© 2022. The Author(s).)

Background and Objective: Salt stress is one of the most important abiotic stresses affecting the yield and quality of tobacco (Nicotiana tabacum). Thymol (a natural medicine) has been widely used in medical research because of its antibacterial and anti-inflammatory activities. However, the influence of thymol on the root growth of tobacco is not fully elucidated. In this study, the regulatory effects of different concentrations of thymol were investigated., Methodology: Here, histochemical staining and biochemical methods, non-invasive micro-test technology (NMT), and qPCR assay were performed to investigate the effect of thymol and mechanism of it improving salinity tolerance in tobacco seedlings., Results: In this study, our results showed that thymol rescued root growth from salt stress by ameliorating ROS accumulation, lipid peroxidation, and cell death. Furthermore, thymol enhanced contents of NO and GSH to repress ROS accumulation, further protecting the stability of the cell membrane. And, thymol improved Na + efflux and the expression of SOS1, HKT1, and NHX1, thus protecting the stability of Na + and K + ., Conclusion: Our study confirmed the protecting effect of thymol in tobacco under salt stress, and we also identified the mechanism of it, involving dynamic regulation of antioxidant system and the maintenance of Na + homeostasis. It can be a new method to improve salinity tolerance in plants., (© 2022. The Author(s).)

Avena fatua and A. ludoviciana (commonly known as wild oats) are the most problematic winter grass species in fallows and winter crops in the northeast region of Australia. A series of experiments were conducted to evaluate the performance of glyphosate and alternative post-emergence herbicides on A. fatua and A. ludoviciana. This study reports the world's first glyphosate-resistant (GR) biotypes of A. fatua and A. ludoviciana. The glyphosate dose required to kill 50% of the plants (LD50) and to reduce 50% of the biomass (GR50) for the GR biotype of A. fatua was 556 g a.e./ha and 351 g a.e./ha, respectively. These values for A. ludoviciana were 848 g a.e./ha and 289 g a.e./ha. Regardless of the growth stage (3-4 or 6-7 leaf stages), clethodim (120 g a.i./ha), haloxyfop (78 g a.i./ha), pinoxaden (20 g a.i./ha), and propaquizafop (30 g a.i./ha) were the best alternative herbicide options for the control of A. fatua and A. ludoviciana. The efficacy of butroxydim (45 g a.i./ha), clodinafop (120 g a.i./ha), imazamox + imazapyr (36 g a.i./ha), and paraquat (600 g a.i./ha) reduced at the advanced growth stage. Glufosinate (750 g a.i./ha), flamprop (225 g a.i./ha), and pyroxsulam + halauxifen (20 g a.i./ha) did not provide effective control of Avena species. This study identified alternative herbicide options to manage GR biotypes of A. fatua and A. ludoviciana., Competing Interests: The authors have declared that no competing interests exist.

Background: Our previous study has demonstrated that the transcription of AchnKCS involved in suberin biosynthesis was up-regulated by exogenous abscisic acid (ABA) during the wound suberization of kiwifruit, but the regulatory mechanism has not been fully elucidated., Results: Through subcellular localization analysis in this work, AchnbZIP29 and AchnMYB70 transcription factors were observed to be localized in the nucleus. Yeast one-hybrid and dual-luciferase assay proved the transcriptional activation of AchnMYB70 and transcriptional suppression of AchnbZIP29 on AchnKCS promoter. Furthermore, the transcription level of AchnMYB70 was enhanced by ABA during wound suberization of kiwifruit, but AchnbZIP29 transcription was reduced by ABA., Conclusions: Therefore, it was believed that ABA enhanced the transcriptional activation of AchnMYB70 on AchnKCS by increasing AchnMYB70 expression. On the contrary, ABA relieved the inhibitory effect of AchnbZIP29 on transcription of AchnKCS by inhibiting AchnbZIP29 expression. These results gave further insight into the molecular regulatory network of ABA in wound suberization of kiwifruit., (© 2022. The Author(s).)

Agricultural soils are under threat of toxic metal/metalloid contamination from anthropogenic activities, leading to excessive accumulation of arsenic (As), cadmium (Cd), lead (Pb), and mercury (Hg) in food crops that poses significant risks to human health. Understanding how these toxic metals and their methylated species are taken up, translocated, and detoxified is prerequisite to developing strategies to limit their accumulation for safer food. Toxic metals are taken up and transported across different cellular compartments and plant tissues via various transporters for essential or beneficial nutrients, e.g. As by phosphate and silicon transporters, and Cd by manganese (Mn), zinc (Zn), and iron (Fe) transporters. These transport processes are subjected to interactions with nutrients and the regulation at the transcriptional and post-translational levels. Complexation with thiol-rich compounds, such as phytochelatins, and sequestration in the vacuoles are the common mechanisms for detoxification and for limiting their translocation. A number of genes involved in toxic metal uptake, transport, and detoxification have been identified, offering targets for genetic manipulation via gene editing or transgenic technologies. Natural variations in toxic metal accumulation exist within crop germplasm, and some of the quantitative trait loci underlying these variations have been cloned, paving the way for marker-assisted breeding of low metal accumulation crops. Using plants to extract and remove toxic metals from soil is also possible, but this phytoremediation approach requires metal hyperaccumulation for efficiency. Knowledge gaps and future research needs are also discussed., (Copyright © 2021 The Author. Published by Elsevier Inc. All rights reserved.)

Ozone (O 3 ) is a damaging air pollutant to crops. As one of the most reactive oxidants known, O 3 rapidly forms other reactive oxygen species (ROS) once it enters leaves through stomata. Those ROS in turn can cause oxidative stress, reduce photosynthesis, accelerate senescence, and decrease crop yield. To improve and adapt our feed, fuel, and food supply to rising O 3 pollution, a number of Free Air Concentration Enrichment (O 3 -FACE) facilities have been developed around the world and have studied key staple crops. In this review, we provide an overview of the FACE facilities and highlight some of the lessons learned from the last two decades of research. We discuss the differences between C 3 and C 4 crop responses to elevated O 3 , the possible trade-off between productivity and protection, genetic variation in O 3 response within and across species, and how we might leverage this observed variation for crop improvement. We also highlight the need to improve understanding of the interaction between rising O 3 pollution and other aspects of climate change, notably drought. Finally, we propose the use of globally modeled O 3 data that are available at increasing spatial and temporal resolutions to expand upon the research conducted at the limited number of global O 3 -FACE facilities., (© 2021 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This article has been contributed to by US Government employees and their work is in the public domain in the USA.)

Environmental stresses may alter the nutritional profile and economic value of crops. Chemical fertilizers and phytohormones are major sources which can enhance the canola production under stressful conditions. Physio-biochemical responses of canola altered remarkably with the use of nitrogen/phosphorus/potassium (N/P/K) fertilizers and plant growth regulators (PGRs) under drought stress. The major aim of current study was to evaluate nutritional quality and physio-biochemical modulation in canola (Brassica napus L.) from early growth to seed stage with NPK and PGRs in different water regimes. To monitor biochemical and physiological processes in canola, two season field experiment was conducted as spilt plot under randomized complete block design (RCBD) with four treatments (Control, Chemical fertilizers [N (90 kg/ha), P and K (45 kg ha-1)], PGRs; indole acetic acid (IAA) 15g ha-1, gibberellic acid (GA3) 15g ha-1 and the combination of NPK and PGRs] under different irrigations regimes (60, 100, 120, 150 mm evaporations). Water stress enhanced peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), polyphenol oxidase (PPO), soluble sugar, malondialdehyde (MDA), proline contents as well as leaf temperature while substantially reduced leaf water contents (21%), stomatal conductance (50%), chlorophyll contents (10-67%), membrane stability index (24%) and grain yield (30%) of canola. However, the combined application of NPK and PGR further increased the enzymatic antioxidant pool, soluble sugars, along with recovery of leaf water contents, chlorophyll contents, stomatal conductance and membrane stability index but decreased the proline contents and leaf temperature at different rate of evaporation. There is positive interaction of applied elicitors to the water stress in canola except leaf area. The outcomes depicted that the combination of NPK with PGRs improved the various morpho-physiological as well as biochemical parameters and reduced the pressure of chemical fertilizers cost about 60%. It had also reduced the deleterious effect of water limitation on the physiology and grain yield and oil contents of canola in field experiments., Competing Interests: The authors have declared that no competing interests exist.

The effects of polyaspartic acid and different controlled-release fertilizers with urea on dry matter accumulation and distribution, nitrogen absorption and accumulation, and the activities of enzymes involved nitrogen metabolism and yield of corn were studied by using xianyu (XY688), a maize nitrogen efficient cultivar, and Jifeng NO.2 (JF2), a maize nitrogen-inefficient cultivar, as experimental materials and through random blocks experimental design in 2019. For XY688, polyaspartic acid chelated nitrogen fertilizer (PASPN) had the highest yield, which was 21.34% higher than N0 treatment. For JF2, it also had the highest yield under PASPN combined urea treatment, which was 23.44% higher than N0 (no nitrogen fertilizer), and JF2 had a 9.7% lower yield under XY688 treatment. For XY688, PASPN treatment had the largest nitrogen uptake in grain, up to 3.14 kg/hm 2 , and PASPN treatment increased 17.4% compared with N0. For JF2, grain nitrogen uptake was also the highest under PASPN treatment, which was significantly different from other treatments. Nitrogen uptake was 3.16 kg/hm 2 , which increased 37.4% compared with N0. Compared with JF2, XY688 showed higher nitrogen uptake efficiency, nitrogen utilization efficiency, and partial nitrogen productivity. For XY688, the highest nitrogen absorption efficiency was SU3 (slow-release urea and ordinary urea) treatment (0.36 kg/kg). The partial nitrogen productivity and harvest index of PASPN treatment were the highest and significantly different from other treatments. The partial nitrogen productivity of PASPN treatment was 57.02 kg/kg. These results can provide help for the further researches of the rational utilization and absorption of nitrogen fertilizer.

This review aims to elucidate the state of the art of microalgae-based biostimulants as a tool in agriculture by summarizing the biologically active compounds factors that influence the use of microalgae biostimulants and their application methods in the field. Additionally, we examined the factors that support the use of microalgal biostimulants to face abiotic and biotic stress in crop plants. The use of microalgae in crop production and the benefits of seed preparation, foliar application, soil drenching, and hydroponic treatments were discussed. Furthermore, the use of these biostimulants in crop plants and their multiple benefits such as, better rooting, higher crop, fruit yields, drought and salinity tolerance, photosynthetic activity and pathogen resistance was thoroughly presented. The present situation of microalgal biostimulants and their difficulties in the market was analyzed, as well as the perspectives of their use. However, data shows that microalgal derived biostimulants can be used as an alternative for the protection of crops and plant growth regulators and play a significant key role in increasing the levels of production, yield and health of crops. Special interest needs to focus on investigating more microalgae species and their biological active compound factors, due to the largely untapped field. Perspectives regarding future research lines and development priorities were included., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)

The phytotoxicity and eco-compatibility of essential oils (EOs) from Eucalyptus gunnii (EG) and E. pulverulenta 'Baby Blue' (EP), cultivated in Italy for their cut foliage, were investigated. Leaf micromorphology, EOs phytochemical characterization, and phytotoxicity were analysed. EP revealed a significantly higher oil gland density and a higher EO yield with respect to EG. In both EOs, 1,8-cineole was the major compound (~75%), followed by α-pinene in EG (13.1%) and eugenol in EP (7.5%). EO phytotoxicity was tested on both weeds ( Lolium multiflorum , Portulaca oleracea ) and crops ( Raphanus sativus , Lactuca sativa , Lepidium sativum , Solanum lycopersicum , Pisum sativum, Cucumis sativus ). EG EO inhibited germination of P. oleracea , R. sativus , and S. lycopersicum seeds (ranging from 61.5 to 94.6% for the higher dose used), while affecting only radical elongation in S. lycopersicum (ranging from 66.7 to 82.6%). EP EO inhibited germination of P. oleracea and R. sativus (ranging from 41.3 to 74.7%) and affected radical elongation of L. sativum and L. multiflorum (ranging from 57.4 to 76.0%). None of the EOs affected the germination and radical growing of L. sativa , P. sativum , and C. sativus . Moreover, EP EO was more active than EG EO in inhibiting α-amylase, a key enzyme for seed growth regulation. Brine shrimp lethality assay showed that both EOs are safe for aquatic organisms, suggesting their high eco-compatibility. The data collected provide useful information for future applications of these EOs in agriculture as safe and selective bioherbicides.

Arsenic (As) contamination is a serious threat to agriculture and human health worldwide. It can adversely affect the growth attributes of food crops. On the other hand, using thiourea (TU) to ameliorate As stress is an economically consistent approach. However, there is a knowledge gap regarding the combined use of TU and Sewage sludge (SS). SS is considered important, unutilized biomass. It can be used as a fertilizer that has high organic matter and nutrients. Therefore, the current study was performed to evaluate TU and SS sole and combined responses under As toxicity on two wheat genotypes (Markaz 19 and Ujala 16). There were four treatments control (As 50 mg kg-1), SS (30 g kg-1)+TU (6.5 mM)+As, TU+As and SS+As applied with four replications. Results revealed that SS+TU performed significantly better over SS, TU and control for improvement in root and shoot fresh and dry weight of wheat varieties Markaz 19 and Ujala 16 under As toxicity. A significant decrease in POD, SOD and APX of Markaz 19 and Ujala 16 also validated the effective functioning of SS+TU over control. The maximum increase of 71 and 77% was noted in phosphorus, where SS+TU was applied over control in Markaz 19 and Ujala 16, respectively. In conclusion, SS+TU is a better approach than the sole application of SS and TU under As contamination for improvement in wheat growth attributes. More investigations are recommended at the field level under different As contamination and agro-climatic zones to declare SS+TU an effective amendment to mitigate As toxicity in wheat., Competing Interests: The authors have declared that no competing interests exist.

To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M1 plants and their descendants were phenotyped for a wide range of traits leading to the identification of 124 mutants having variations in key agro-morphological traits, and 106 mutants exhibiting variation for physiological traits. Higher yield is the ultimate goal of crop improvement and we identified 574 mutants having higher yield compared to wild type by having better yield attributing traits. Further, a total of 50 mutants showed better panicle exertion phenotypes as compared to Samba Mahsuri leading to enhancement of yield. Upon rigorous screening for three major biotic stresses, 8 mutants showed enhanced tolerance for yellow stem borer (YSB), and 13 different mutants each showed enhanced tolerance for sheath blight (ShB) and bacterial leaf blight (BLB), respectively. In addition, screening at multiple locations that have diverse field isolates identified 3, 3, and 5 lines for tolerance to ShB, YSB and BLB, respectively. On the whole, 1231 desired mutant lines identified at M2 were forwarded to an advanced generation (M5). PCR based allele mining indicated that the BLB tolerant mutants have a different allele than the reported alleles for well-known genes affecting bacterial blight resistance. Whole genome re-sequencing revealed substantial variation in comparison to Samba Mahsuri. The lines showing enhanced tolerance to important biotic stresses (YSB, ShB and BLB) as well as several economically important traits are unique genetic resources which can be utilized for the identification of novel genes/alleles for different traits. The lines which have better agronomic features can be used as pre-breeding lines. The entire mutant population is maintained as a national resource for genetic improvement of the rice crop., Competing Interests: NO authors have competing interests.

A great amount of data covering a wide variety of plant species and experimental conditions has demonstrated the beneficial actions that melatonin exerts on many aspects of plant development, including germination, photosynthesis and water economy. Melatonin behaves especially well as a plant biostimulator against biotic and abiotic stressors, increasing stress tolerance. The present contribution sets out possible future multidisciplinary studies, in which the impact of using melatonin with respect to agriculture, food technology, human nutrition and the environment needs to be clearly established. In crops, the effective dose and best formulations for individual plant species and cultivation conditions should be studied. As regards post-harvest, the focus should be on the half-life time of melatonin in fruits and water-residue treatments. Detailed studies are lacking on the human intake of phytomelatonin in different diets. Studies on the metabolization of phytomelatonin and the combined effect with other phytonutrients such as carotenoids, chlorophylls, flavonoids, fibers, etc., would also be of interest. In soils, the possible interaction between melatonin and microbiome and non-vertebrate animals is of primordial interest. In terms of the environment, although melatonin is classified as a non-hazardous agent, its limitations as a possible animal hormone disruptor have been suggested. Specific studies on the permanence of melatonin in plant tissues, plant by-products, soil, freshwater and honeybees, amongst others, are proposed to obtain crucial information. © 2021 Society of Chemical Industry., (© 2021 Society of Chemical Industry.)

The soybean aphid Aphis glycines Matsumura (Hemiptera: Aphididae) is a primary pest of soybeans and poses a serious threat to soybean production. Our studies were conducted to understand the effects of different concentrations of insecticides (imidacloprid and thiamethoxam) on A. glycines and provided critical information for its effective management. Here, we found that the mean generation time and adult and total pre-nymphiposition periods of the LC50 imidacloprid- and thiamethoxam-treatment groups were significantly longer than those of the control group, although the adult pre-nymphiposition period in LC30 imidacloprid and thiamethoxam treatment groups was significantly shorter than that of the control group. Additionally, the mean fecundity per female adult, net reproductive rate, intrinsic rate of increase, and finite rate of increase of the LC30 imidacloprid-treatment group were significantly lower than those of the control group and higher than those of the LC50 imidacloprid-treatment group (P < 0.05). Moreover, both insecticides exerted stress effects on A. glycines, and specimens treated with the two insecticides at the LC50 showed a significant decrease in their growth rates relative to those treated with the insecticides at LC30. These results provide a reference for exploring the effects of imidacloprid and thiamethoxam on A. glycines population dynamics in the field and offer insight to agricultural producers on the potential of low-lethal concentrations of insecticides to stimulate insect reproduction during insecticide application., Competing Interests: This manuscript has not been published or presented elsewhere in part or in entirety and is not under consideration by another journal. We have read and understood your journal’s policies, and we believe that neither the manuscript nor the study violates any of these. There are no conflicts of interest to declare.

This review considers potential approaches to solve an important problem concerning the impact of applied pesticides of various classes on living organisms, mainly agricultural crops used as food. We used the method of multi-residual determination of several pesticides in agricultural food products with its practical application for estimating pesticides in real products and in model experiments. The distribution of the pesticide between the components of the soil-plant system was studied with a pesticide of the sulfonylureas class, i.e., rimsulfuron. Autoradiography showed that rimsulfuron inhibits the development of plants considered as weeds. Cereals are less susceptible to the effects of pesticides such as acetamiprid, flumetsulam and florasulam, while the development of legume shoots was inhibited with subsequent plant death.

The toxic metalloid arsenic (As), is a major pollutant of soil and water, imposing severe health concerns on human lives. It enters the food chain mainly through As-contaminated crops. The uptake, translocation and accumulation of As in plant tissue are often controlled by certain soil-inhabiting microbial communities. Among them, indigenous, free-living As-resistant plant growth-promoting rhizobacteria (PGPR) plays a pivotal role in As-immobilization. Besides, the plant's inability to withstand As after a threshold level is actively managed by these PGPR increasing As-tolerance in host plants by a synergistic plant-microbe interaction. The dual functionality of As-resistant PGPR i.e., phytostimulation and minimization of As-induced phytotoxic damages are one of the main focal points of this review article. It is known that such PGPR having the functional arsenic-resistant genes (in ars operon) including As-transporters, As-transforming genes contributed to the As accumulation and detoxification/transformation respectively. Apart from assisting in nutrient acquisition and modulating phytohormone levels, As-resistant PGPR also influences the antioxidative defense system in plants by maneuvering multiple enzymatic and non-enzymatic antioxidants. Furthermore, they are effective in reducing membrane damage and electrolyte leakage in plant cells. As-induced photosynthetic damage is also found to be salvaged by As-resistant PGPR. Briefly, the eco-physiological, biochemical and molecular mechanisms of As-resistant PGPR are thus elaborated here with regard to the As-exposed crops., (Copyright © 2021 Elsevier GmbH. All rights reserved.)

Biochar is a carbon-rich product, which is processed by pyrolyzing biomass to improve soil properties and maintain environmental sustainability. This study aim was to investigate the effect of biochar and inorganic fertilizer on soil properties, growth, and yield. Four treatments and four replications have been used for the experimental plots using Randomized Complete Block Design (RCBD). Soil physiochemical properties have been investigated based on soil samples within 0-30 cm depth in each plot. The two types of biochar (grass and chat waste) have been used for the treatments. The pyrolyzing temperature required for grass and chat waste is 250°C and 1100°C, respectively. The plant height, total yield, and the marketable and nonmarketable yield of onion have been examined. The analysis showed that treatment with grass biochar and inorganic fertilizer had a similar effect on soil properties but chat (C atha edulis ) biochar had a lower effect on soil properties. The total yield and days to 70% maturity have been increased due to the cumulative treatment of grass biochar and inorganic fertilizer. The biochar of grass and inorganic fertilizer have been significantly increased in marketable bulb yield, but unmarketable yield becomes decreased. The chat waste and controlled treatments shown an increased unmarketable yield and declined marketable bulb yield. Overall, biochar can substitute the inorganic fertilizer, which can help to improve the w soil fertility and environmental sustainability. Therefore, biochar has a win-win solution., Competing Interests: The authors declare that they have no conflicts of interest regarding the publication of this study., (Copyright © 2021 Abreham Berta Aneseyee and Tekilil Wolde.)