Search

Job shop scheduling problem (JSP) is the most typical scheduling problem, In the process of JSP based on genetic algorithm (GA), large amounts of data will be produced. Mining them to find the useful information is necessary. In this paper dividing, hashing and array (DHA) association rule mining algorithm is used to find the frequent itemsets which contained in the process, and extract the corresponding association rules. Concept hierarchy is used to interpret the rules, and lots of useful rules appeared. It provides a new way for JSP study.

In real production processing, job shop scheduling problem (JSP) is often express as dynamic scheduling problem. In this article a hybrid genetic algorithm and handling strategies are used for real job shop scheduling problem. It gives the scheduling result with the appropriate handling strategies to the stochastic events such as equipment breakdown and urgent orders. The data of Shanghai Shen Mo Die & Mold Manufacturing Co., Ltd (ShenMo) is used for the application of dynamic scheduling simulation, and the results of which show that the proposed method can satisfactorily solve the stochastic events of scheduling.

To avoid premature and sensitivity of operator parameters selecting of Standard genetic algorithm (SGA) and simulated annealing genetic algorithm (SAGA), a parallel hybrid genetic algorithm based on multi-group (hybrid GA) is presented. The algorithm combines the ideas of parallel computation, simulated annealing and genetic algorithm, and uses orthogonal test table selecting operator parameters to improve the efficiency and robust of the algorithm. And benchmark example of job shop scheduling problem (JSP) is used to validate the effectiveness of the algorithm. Results show the hybrid genetic algorithm converges quickly with small impact to operator parameters.

Antisense oligonucleotides-induced local blockade of T-bet expression leads to airway inflammation in rats 1

To explore the effect of Astragalus membranaceus (AM) on T-helper cell type 1 (Thl) specific transcription factor T-box expressed in T cells (T-bet) expression and Thl/Th2 equilibrium.The levels of T-bet mRNA in peripheral blood mononuclear cells (PBMCs) from 15 patients with asthma and 15 healthy subjects were determined by reverse transcription-polymerase chain reaction (RT-PCR). PBMCs in asthma patients were incubated with AM and then the concentration of interferon gamma (IFN-gamma) and interleukin-4 (IL-4) in the supernate before and after AM intervention were determined by ELISA. The numbers of CD4 + CCR3 + and CD4 + CCR5 + cells were counted by flow cytometry.The expression of T-bet mRNA and the level of IFN-gamma were lower, but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 microg/ml) intervention, the former two parameters raised and showed a positive correlation between them, while the level of IL-4 was decreased. The mean percentage of CD4 + CCR3 + cells in asthma patients was significantly higher but that of CD4 + CCR5 + cells was lower when compared with those in healthy subjects respectively. After AM intervention, the abnormal change in the two indexes was improved to certain extent, showing a reversing status of Th2 polarization.AM could increase the expression of T-bet mRNA and Thl cytokines such as IFN-Y, and might reverse the Th2 predominant status in asthma patients.

To explore whether local blockade of T-box expressed in T cells (T-bet) expression in the lungs could lead to airway inflammation.Twenty-four rats were randomly divided into 4 groups: saline group, ovalbumin (OVA)-sensitized group, nonsense group, and the antisense group. The OVA-sensitized rats were sensitized and challenged with OVA, and the rats in the nonsense and antisense groups were subjected to an aerosol delivery of the nonsense and antisense oligonucleotides (AS-ODN) of T-bet (0.1%, w/v). The levels of interferon-gamma (IFN-gamma), interleukin-4 (IL-4), and IL-5 in the bronchoalveolar lavage fluid (BALF) were detected by ELISA, and the mRNA and the protein expression of T-bet and GATA-3 genes were examined by in situ hybridization and Western blot analysis, respectively.The expression of T-bet mRNA and protein in the lungs of the rats in the antisense group were inhibited effectively. The lungs of the rats in the antisense and OVA-sensitized groups showed eosinophil and lymphocyte inflammatory infiltration, and eosinophilia located predominantly around the airways. The number of GATA-3 mRNA-positive cells and the level of GATA-3 protein in the lungs of the rats in the antisense and the OVA-sensitized groups significantly increased. The level of IL-4 and IL-5 in the BALF in the antisense and OVA-sensitized groups were elevated, but the level of IFN-gamma decreased markedly.Antisense ODN-induced local blockade of T-bet expression leads to airway inflammation with a selective alteration in patterns of cytokine expression and recruitment of eosinophil cells similar to that in the OVA-sensitized animals.