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Highly fluorogenic tetrazine bioorthogonal probes emitting at near‐infrared wavelengths are in strong demand for biomedical imaging applications. Herein, we have developed a strategy for forming a palette of novel Huaxi‐Fluor probes in situ, whose fluorescence increases hundreds of times upon forming the bioorthogonal reaction product, pyridazine. The resulting probes show large Stokes shifts and high quantum yields. Manipulating the conjugate length and pull–push strength in the fluorophore skeleton allows the emission wavelength to be fine‐tuned from 556 to 728 nm. The highly photo‐stable and biocompatible probes are suitable for visualizing organelles in live cells without a washing step and for imaging of tumors in live small animals to depths of 500 μm by two‐photon excitation. [ABSTRACT FROM AUTHOR]

BACKGROUND: The use of cold therapy to promote rehabilitation after total knee arthroplasty has a certain theoretical basis, but whether cold therapy can reduce bleeding, reduce pain, and promote early recovery of activity is still controversial. OBJECTIVE: To evaluate the effectiveness of cold therapy in total knee arthroplasty in reducing postoperative bleeding, reducing pain, and promoting recovery of motion range using meta-analysis. METHODS: Medline, EMBASE, Cochrane, China Biomedical Literature Database, CNKI, Wanfang and other databases were searched to find randomized controlled trials of cold therapy and other rehabilitation therapies after total knee arthroplasty. Data ware extracted. Statistical analysis was performed using Revman 5.3. Effect values were combined. RESULTS AND CONCLUSION: (1) A total of 10 randomized controlled trials were included, including 1 070 patients. The experimental group received cold therapy after total knee arthroplasty, and the control group received other rehabilitation therapies. (2) Meta-analysis showed that compared with other rehabilitation therapies, cold therapy after replacement alleviated postoperative pain [MD=-0. 75, 95%C/ (-1.29, -0.21 ), P=0.006], reduced hemoglobin drop [MlP-12.11, 95%C/ (-17.66, -6.56), P < 0.000 1]. However, cold therapy could not reduce the amount of opioids usage [MD=0.01, 95%C/ (-0.15, -0.16), P=0.92], or improve joint motion range [MD=6.58, 95%C/ (-0.54, 13. 70), P=0.07]. (3) The results show that the application of cold therapy after total knee arthroplasty can effectively relieve pain and reduce the drop of postoperative hemoglobin, but has no significant effect on reducing the application of opioid analgesics or improving motion range. [ABSTRACT FROM AUTHOR]

The treatment of colorectal cancer is always a major challenge in the field of cancer research. The number of estimated new cases of colorectal cancer worldwide in 2020 is 1 148 515, and the estimated number of deaths is 576 858, revealing that mortality accounted for approximately half of the disease incidence. The development of new drugs and strategies for colorectal cancer treatment is urgently needed. Thermosensitive injectable hydrogel PDLLA-PEG-PDLLA (PLEL) loaded with cabazitaxel (CTX) is used to explore its anti-tumor effect on mice with orthotopic colorectal cancer. CTX/PLEL is characterized by a solution state at room temperature and a hydrogel state at physiologic temperature. The excipients MPEG-PCL and PDLLA-PEG-PDLLA have good biocompatibility and biodegradability. The simple material synthesis and preparation process renders this system cost-effective and more conducive to clinical transformation. An orthotopic colorectal cancer model is established by transplantation subcutaneous tumors onto the cecum of mice. According to the results of experiments in vivo, CTX/PLEL significantly inhibits orthotopic colorectal cancer and liver metastasis in mice. The results indicate that CTX/PLEL nanoparticle preparations have high security and excellent anti-tumor effects, and have great application potential in colorectal cancer therapy., (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)