1. Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella
- Author
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Antonio Olavo Cardoso Jorge, Mariella Vieira Pereira Leão, Luciane Dias de Oliveira, Rodnei Dennis Rossoni, Juliana Campos Junqueira, Patrícia Pimentel de Barros, Felipe Eduardo de Oliveira, Barbara Evelyn Begnini, Universidade Estadual Paulista (Unesp), University of Taubaté, Oliveira, Luciane https://orcid.org/0000-0001-9956-7768, de Barros, Patricia Pimentel https://orcid.org/0000-0003-4885-2811, De Barros, Patricia Pimentel https://orcid.org/0000-0003-4885-2811, Rossoni, Rodnei D https://orcid.org/0000-0002-9977-3040, Junqueira, Juliana Campos https://orcid.org/0000-0001-6646-6856, Oliveira, Felipe https://orcid.org/0000-0003-3026-646X, Oliveira, Luciane/V-6606-2019, de Barros, Patricia Pimentel/M-8160-2019, De Barros, Patricia Pimentel/B-3496-2016, Rossoni, Rodnei Dennis/P-3263-2019, Rossoni, Rodnei D/K-2390-2013, and Junqueira, Juliana Campos/Q-3108-2019
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0301 basic medicine ,Hemocytes ,Epidemiology ,Colony Count, Microbial ,Probiotic ,Mice ,Lactobacillus acidophilus ,Lactobacillus ,Yeasts ,Lactic acid bacteria ,Innate ,Candida ,biology ,Lacticaseibacillus rhamnosus ,Candidiasis ,food and beverages ,Lacticaseibacillus paracasei ,Corpus albicans ,Galleria mellonella ,Lepidoptera ,Infectious Diseases ,Cytokines ,Infection ,Lactobacillus paracasei ,Cell Survival ,030106 microbiology ,Immunology ,Mycology ,Microbiology ,Biofilm Formation ,03 medical and health sciences ,Lactobacillus rhamnosus ,In vivo ,Candida-Albicans ,Animals ,Pathogenicity ,Viability assay ,Macrophages ,biology.organism_classification ,Survival Analysis ,Immune ,Disease Models, Animal ,Host-Defense ,030104 developmental biology ,RAW 264.7 Cells ,In-Vitro ,Antifungal Resistance - Abstract
Made available in DSpace on 2019-09-12T16:53:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2017 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Due to the growing number of multi-resistant Candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. This study evaluated the immunomodulation and anti-Candida activity of Lactobacillus rhamnosus (LR), Lactobacillus acidophilus and Lactobacillus paracasei suspensions, either single- or multiple-strain, in mouse macrophages (RAW 264.7) and Galleria mellonella (GM). Mouse macrophages were activated by different lactobacilli suspensions and challenged with C albicans (CA). Tumor necrosis factor (TNF)-alpha, interleukin IL-beta, IL-6 and IL-17 production and cell viability were investigated. LR was the best suspension for stimulating all evaluated cytokines and thus was used in subsequent in vivo assays. Two C albicans clinical strains, CA21 and CA60, were then added to the GM assays to further confirm the results. LR suspension was injected into the larvae 24 h before challenging with CA. Survival curve, CFU per larva and hemocytes were counted. In the GM, the LR suspension increased the survival rate and hemocyte counts and decreased the CFU per larva counts for all groups. Lactobacilli suspensions presented strain-dependent immunomodulation; however, single suspensions showed better results. Anti-Candida activity was demonstrated by decreased Candida counts in the GM with the use of LR. (C) 2017 Elsevier Ltd. All rights reserved. [de Oliveira, Felipe Eduardo; Rossoni, Rodnei Dennis; de Barros, Patricia Pimentel; Begnini, Barbara Evelyn; Junqueira, Juliana Campos; Cardoso Jorge, Antonio Olavo; de Oliveira, Luciane Dias] Sao Paulo State Univ Unesp, Dept Biosci & Oral Diag, Inst Sci & Technol, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Paulo, Brazil [Pereira Leao, Mariella Vieira] Universidade de Taubaté (Unitau), Sch Med, Inst Basic Biosci, Av Tiradentes 500, BR-12030180 Sao Paulo, Brazil
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- 2017