1. PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20
- Author
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Christos Mavrangelos, Timothy Sadlon, Danika L. Hill, Batjargal Gundsambuu, Heddy Zola, Randall H. Grose, Simon C. Barry, Daniel Bird, Suzanne Bresatz-Atkins, Debbrah J. Millard, Sarah To, Ian C. Nicholson, Doreen Krumbiegel, Nicola Eastaff-Leung, Nicholson, Ian, Mavrangelos, Chris, Bird, A, Bresatz-Atkins, S, Eastaff-Leung, N G, Grose, R, Gundsambuu, Batjargal, Hill, Dan, Millard, D J, Sadlon, Timothy, To, S, Zola, Heddy, Barry, Simon, and Krumbiegel, Doreen
- Subjects
Chemokine ,Immunology ,CCR4 ,Inflammation ,chemical and pharmacologic phenomena ,C-C chemokine receptor type 6 ,Biology ,T-Lymphocytes, Regulatory ,Chemokine receptor ,Cell Movement ,mental disorders ,medicine ,CCL17 ,Humans ,Cell Proliferation ,Glycoproteins ,Chemokine CCL20 ,FOXP3 ,Forkhead Transcription Factors ,hemic and immune systems ,CCL20 ,Phenotype ,biology.protein ,Cytokines ,Leukocyte Common Antigens ,Chemokine CCL17 ,medicine.symptom ,Carrier Proteins ,Immunologic Memory ,psychological phenomena and processes - Abstract
The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites. Refereed/Peer-reviewed
- Published
- 2012