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Guizotia abyssinica (L. f) Cass. (Asteraceae) is native to Ethiopia. This asteracea was discovered in the region of Sétif and M’sila, in northeastern Algeria. Ecological notes are also provided.

The phytochemical profile obtained from LC-ESI-MS/MS analysis of the n-butanol extract (BEHL) from the North African endemic plant Hyacinthoides lingulata (Poir.) Rothm. brought about the identification of ten glycosylated derivatives of apigenin and luteolin flavones. For the same plant extract, in vitro anti-inflammatory (hypotonic induced hemolysis and heat induced haemolysis assay) and antioxidant (DPPH and β-Carotene) activities were evaluated observing high inflammatory inhibition by protecting membrane stability of erythrocyte in both heat (84.70 ± 0.24%) and hypotonic induced hemolysis (79.45 ± 0.12%). A remarkable hemostatic effect was also established by measuring the coagulation time (15.95 ± 1.05 s at a dose of 1 mg/mL) of decalcified plasma related to its phytochemical content. It is the first report on combined chemical components and biological evaluation of this specific plant.

The phytochemical profile obtained from LC-ESI-MS/MS analysis of the

The phytochemical investigation of both chloroform and ethyl acetate extracts of Centaurea microcarpa Coss. & Dur. led to the isolation of a new cyanogenic glucoside 6'-methacrylate prunasin (3) together with seven known compounds: hydroxy-11β,13-dihydro onopordaldehyde (1), β-sitosterol (2), daucosterol (4), nepetin (5), prunasin (6), astragalin (7) and 7-O-β-D-glucopyranosyl centaureidin (8). Their structures were established by spectral analysis, mainly UV, IR, ESI-MS, 1D & 2D-NMR experiments (COSY, HSQC, HMBC and ROESY).

This is the first report on the phytochemistry and antioxidant activity of ethyl acetate and n- butanol extracts from an Algerian endemic plant Verbascum atlanticum Batt. (Scrophulariaceae). Both extracts were subjected to a phytochemical study by semi-preparative HPLC, which led to the isolation and identification of nine compounds: methyl linolenate (1), methyl linoleate (2), Phytol-1(3), Martynoside (4), Isomartynoside (5), Cis-martynoside (6), Ilwensisaponin C (7), Ilwensisaponin B (8), Ilwensisaponin A (9). In addition, the fractions from both extracts were analysed by LC-UV-MS and HRESI-MS. This later revealed the presence of eight other metabolites by using a comparison with known microbial metabolites data. Finally, both extracts were estimated for their phenolic and flavonoid contents as well as the evaluation of their antioxidant activity using five different assays DPPH, CUPRAC, reducing power, β-carotene bleaching and superoxide DMSO alkaline. The results showed that the ethyl acetate extract had the most antioxidant effect.

Background: The antioxidant activity and the total phenolic and flavonoid contents of the derived extracts (chloroform, ethyl acetate and n-butanol) of the 70% hydroalcoholic extract of the aerial parts of Centaurea solstitialis growing in Algeria was assessed. The active extracts were selected for phytochemical investigations. Methods: The antioxidant capabilities of the extracts were assessed using 1, 1-diphenyl-2- picrylhydrazyl radical (DPPH•) scavenging and Cupric Ion Reducing Antioxidant Capacity (CUPRAC) assays. Butylhydroxyanisole (BHA), butylhydroxytoluene (BHT) and α –tocopherol were used as positive controls. The total phenolic content and total flavonoid content of the extracts were determined as gallic acid equivalents and quercetin equivalents, respectively. Chromatographic methods were used to isolate the secondary metabolites and spectrometric and spectroscopic methods were used to determine their chemical structures. Results: The ethyl acetate extract exhibited the highest antioxidant activities followed by the n-butanol extract. The highest phenolic and flavonoid contents were found in the n-butanol extract. Phytochemical study of the ethyl acetate and n-butanol extracts led to the isolation of an undescribed guaianolide named 3-(4-hydroxybenzoyl)-cynaratriol and a known sesquiterpene lactone along with three known flavonoid glycosides. Their structures were established by spectral analyzes mainly high resolution electrospray ionisation mass spectrometry (HR-ESIMS) and 1D and 2D nuclear magnetic resonance experiments. Conclusion: The extracts of aerial parts of C. solstitialis showed significant antioxidant activities. An undescribed sesquiterpene lactone and four known secondary metabolites were isolated from the most active extracts.

The study was performed on the dichloromethane (DCM), ethyl acetate (EAc) and

This is the first report on the phytochemistry and antioxidant activity of ethyl acetate and

The study was performed on the dichloromethane (DCM), ethyl acetate (EAc) and n-butanol (Bu) fractions (F) obtained from the 80% ethanol extract of Linaria scariosa Desf. aerial parts, collected in the North Eastern region of Algeria. Remarkable total phenolic and flavonoid contents were obtained, mainly for EAcF. These results were in accordance with the antioxidant activity of EAcF against DPPH, ABTS, CUPRAC and reducing power tests. DCMF and BuF exhibited significant cholinesterase activity inhibition of BChE and AChE. Moreover, EAcF displayed only moderate antibacterial activities, especially against S. aureus. The biological results were correlated to the chemical components, deduced by both GC-MS analysis of the fractions and the isolation of hemipholin, pectolinarigenin, antirride, antirrinoside, pectolinarin and linariosise, some of which known to exhibit potent effects on the tested biological activities. The study provides the first biological and chemical investigation on Linaria scariosa Desf (unresolved name).

Note sur une nouvelle station d’Anacyclus monanthos subsp. cyrtolepidioides (Pomel) Humphries dans la région du Chott el Hodna (M’sila, Algérie). Mots clés: Asteraceae, Anacyclus monanthos, espèce endémique, Algerie. Key words: Asteraceae, Anacyclus monanthos, endemic species, Algeria.

The chemical composition of the essential oil from the aerial parts of Anacyclus monanthos subsp. cyrtolepidioides (Pomel) Humphries (Asteraceae) growing in a semi-arid region of Algeria was investigated for the first time. The essential oil was obtained by hydrodistillation and fully characterized by gas chromatography-mass spectrometry (GC-MS). A total of 97 compounds were identified. The essential oil was found to be rich in trans-chrysanthenyl acetate (9.8 ± 2.0%), (E)-β-farnesene (7.4 ± 1.5%), germacrene D (6.9 ± 1.3%) and myristicin (4.8 ± 0.8%).

Aim and objective: This study aimed to quantify phenolic compounds in ethyl acetate and n-butanol extract of Centaurea choulettiana Pomel (Asteraceae) leaves and flowers; compare the antioxidant activity of their extracts, identification and quantification of their phenolic acids. Materials and methods: Both organs extracts of Centaurea choulettiana Pomel were investigated and evaluated for their potential antioxidant properties using total phenolics and flavonoids content, DPPH radical scavenging and lipid peroxidation inhibition assays. HPLC-TOF/MS analyses were carried out to identify and quantify some phenolic acids. Results: The amounts of phenolic and flavonoid content were higher in ethyl acetate extract of leaves (325.81 ± 0.038 mgGAE and 263.73 ± 0.004 mgQE /g of extract) respectively. Besides, this extract exhibited the most powerful effect on the DPPH radical scavenging activity with (96.54%), on lipid peroxydation inhibition (64.17%). Ethyl acetate extract of leaves and flowers were found to contain almost the same phenolic compounds, with the leaves having the highest values. Chlorogenic acid was detected in the n-butanol extract of flowers with the highest concentration 17.78 mg/kg plant. Conclusion: The ethyl acetate extract of leaves of Centaurea choulettiana possesses strong antioxidative properties in vitro. They are confirmed by high polyphenols and flavonoids content. The HPLC-TOF/MS analysis reveals the presence of 4-hydroxybenzoic acid, gentisic acid, chlorogenic acid, caffeic acid, vanillic acid, p-Coumaric acid, ferulic acid, salicylic acid and protocatechuic acid.

The genus Linaria belongs to the Scrophulariaceae family and comprises about 200 species [1]. It is represented by 39 in Algeria [2]; several species have been used in folk medicine as tonics, antiscorbutics, laxatives, antidiabetics, and diuretics [3, 4]. Previous work on this genus has shown the presence of iridoids, diterpenoids, flavonoids, alkaloids, and phenylethanoids [4–9].

Ethnopharmacological relevance The main aim of this study was to identify, catalogue and document the large number of wild medicinal plants used in the M'Sila region (northern Algeria) for the treatment of several human pathologies. Another more ambitious aim is to contribute to overcoming the limits of an orally transmitted pharmacopoeia, attempting to exploit the large ethnopharmacology patrimony of the region for further pharmacological purposes. Materials and methods Our field study was carried out over a period of three years (2008–2010). During this period, herbalists were interviewed using semi-structured questionnaires investigating the herbalist as a holder of information (gender, age and educational level) and about wild medicinal plants (local name, uses and part used). In addition, the relative importance value of the species was determined and informant consensus factor (ICF) was calculated for the medicinal plants included in the study. Results A total of 83 herbalists were interviewed; men dominate the practice of traditional medicine in the region. About 41% of them are between 31 and 40 years, and about a third (34%) are illiterate. The traditional herbal knowledge is passed from generation to generation in the verbal form, a writing tradition being almost totally absent. The interviewed herbalists identified and recorded 58 plants species and 50 genera belonging to 27 plant families. Lamiaceae and Asteraceae were the most represented plant families. The aerial parts were the most commonly used plant part, while infusion and decoction were the most common method of traditional drug preparation. Conclusions The survey provides a veritable source of information on the herbalists and wild medicinal plants. Plants which are used in different parts of the world for the treatment of similar diseases may be deemed to be effective in pharmacological terms. These medicinal plants may be incorporated into the healthcare delivery system of the country.

To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts of the aerial parts of Genista ulicina Spach. from Algeria. The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and n-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc extract exhibited good antioxidant activities comparing to the n-BuOH extract. Thus Genista ulicina could be indicated as a plant of phytopharmaceutical importance.

0009-3130/12/4706-1023 2012 Springer Science+Business Media, Inc. 10231) Laboratoire de Phytochimie et Analyses Physico-Chimiques et Biologiques, Departement de Chimie, UniversiteMentouri, Constantine, Route de Ain El Bey, 25000 Constantine, Algerie, fax: 213 31 81 88 83, e-mail: fbenayache@yahoo.fr;2) Laboratoire de Valorisation des Ressources Naturelles, Departement de Chimie, Universite Mentouri, Constantine, Routede Ain El Bey, 25000 Constantine, Algerie; 3) Departement de Biologie, Faculte des Sciences, Universite Mohammed Boudiaf,M Sila, 28000, M Sila, Algerie; 4) Instituto de Productos Naturales y Agrobiologia-C.S.I.C.-Instituto Universitario de Bio-Organica “Antonio Gonzalez”, Universidad de La Laguna, Av. Astrofisico F. Sanchez 3, 38206 La Laguna, Tenerife, Spain.Published in Khimiya Prirodnykh Soedinenii, No. 6, pp. 889–890, November–December 2011. Original article submittedAugust 31, 2010.

710 0009-3130/12/4804-0710 2012 Springer Science+Business Media, Inc.1) Laboratoire de Phytochimie et Analyses Physico-Chimiques et Biologiques, Departement de Chimie, UniversiteMentouri, Constantine, Route de Ain El Bey, 25000, Constantine, Algerie, fax: 213 31 81 88 83, e-mail: mekkiou_ratiba@yahoo.fr;2) Laboratoire de Valorisation des Ressources Naturelles et Synyhese de Substances Biologiquement Actives, UniversiteMentouri, Constantine, Route de Ain El Bey, 25000, Constantine, Algerie, fax: 213 31 81 88 83; 3) Consejo Superior deInvestigaciones Cientificas, Instituto de Productos Naturales y Agrobiologia, Tenerife, Spain. Published in