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Copper oxides species deposited on ceria rods, particles, and cubes were examined for low-temperature oxidation of CO. It was found that the shape of ceria altered the dispersion and chemical state of copper species considerably. CuOx monolayers and bilayers were formed on ceria rods and particles, while multilayers and faceted particles co-existed on ceria cubes. The formation of Cu+ species at the copper–ceria interface involved a significant charge transfer from copper oxides to the ceria surface via a strong electronic interaction, which was more pronounced on ceria rods. The concentrations of surface Cu+ and oxygen vacancies followed the order rods > particles > cubes, in line with their catalytic activity for CO oxidation at 343 K. [ABSTRACT FROM AUTHOR]

RNA interference (RNAi), which can inhibit the expression or transcription of specific genes, is considered to be a revolutionary tool for mediating gene therapy. Small interfering RNA (siRNA) is an important therapeutic agent for RNAi. However, the clinical application of siRNA‐based gene silencing is still limited due to the easy degradation of siRNA during the circulation in the body and the difficulty in delivering siRNA to desired tissues and cells. The rapid development of nanotechnology provides new ideas for siRNA delivery and a large variety of nanocarriers have been designed to solve the problems of siRNA delivery including easy degradation, lack of targeting ability, poor internalization ability, easy to cause induction of immune response and so on. Here, this review introduces the mechanism of RNAi in gene therapy, then summarizes the developments on nanocarriers for siRNA delivery, and specially focuses on the functional modification strategies of nanocarriers to construct multifunctional siRNA delivery vectors. Lastly, the future development and challenges of siRNA delivery vector are discussed, along with the current application of siRNA in tumor treatment clinical trials. It is hoped that this review can provide useful information to help transform siRNA‐mediated gene therapy into clinical application. [ABSTRACT FROM AUTHOR]

The Neoarchean basement rocks of the Qixingtai region in the northwest of western Shandong, eastern North China Craton are divided into western and eastern domains, separated by a ductile shear zone. The western domain is mainly composed of early Neoarchean tonalitic and supracrustal rocks, and the eastern domain is composed of late Neoarchean crustally‐derived granite with diverse early Neoarchean enclaves up to several kilometres long of supracrustal and gneissic TTG‐diorite rocks. This study reports an integrated field, whole‐rock geochemistry and zircon U–Pb‐Hf study on the granites and their enclaves in the eastern domain. The supracrustal enclaves are mainly composed of meta‐basaltic and meta‐ultramafic rocks and are the same in lithological association and formation time as the 2,750–2,700 Ma Liuhang "Formation" in the western area. The TTG‐diorite enclaves can be divided into early and late groups. Magmatic zircons from early TTG‐diorite samples have U–Pb ages of 2,731 ± 17 to 2,748 ± 13 Ma and εHf(t) values of +2.7 to +9.5, whereas trondhjemite samples of the late group have U–Pb zircon ages of 2,588 ± 18 to 2,606 ± 12 Ma and εHf(t) values of +0.6 to +6.5. The Hf isotopic signatures on magmatic zircon from the >2,700 Ma diorite‐tonalites indicate that they are predominantly juvenile crustal additions, probably with minor involvement of older crust in their genesis, as given by tDM (Hf) ages ranging back to 2.92 Ga. The eastern granites range from monzogranite to syenogranite in composition and commonly have negative Eu anomalies, indicating residual plagioclase in the source region. Magmatic zircons from two granite samples have U–Pb ages of 2,516 ± 24 and 2,527 ± 18 Ma and εHf(t) values of +1.0 to +7.0. A fine‐grained syenogranite dyke contains inherited early Neoarchean zircons with εHf(t) values of +1.6 to +7.4. The isotopic signatures of the late Neoarchean granites that dominate the eastern domain show that they were derived from partial melting recycling of older continental crust recognized in the region – early Neoarchean rocks formed as a result of juvenile crustal additions. This is a common signature for late Archean crustal evolution throughout much of the North China Craton, and is the hallmark of some other Archean terranes, such as the Dharwar Craton of south India. The geodynamic explanations for the formation globally of vast amounts of crustally‐derived late Neoarchean granite are discussed. [ABSTRACT FROM AUTHOR]

Obesity has been recognized as a low-grade, chronic inflammatory disease that leads to an increase in obesity-associated disorders, including type 2 diabetes (T2D), fatty liver diseases and cancer. Glucagon-like peptide-1 (GLP-1) is an effective drug for T2D, and it not only has glucose-regulating effects but also has anti-inflammatory effects in obesity. In our previous study, we designed a novel GLP-1 analogue, (E X -4) 2 -Fc, which has been shown to reduce body weight and improve glucose tolerance in vivo. In this study, we observed that (E X -4) 2 -Fc also has anti-inflammatory functions in adipose tissue. After the treatment of diet-induced obesity (DIO) mice with (E X -4) 2 -Fc, we found that the inflammatory response in adipose tissue was significantly attenuated. (Ex-4) 2 -Fc can reduce obesity-associated proinflammatory cytokine levels and macrophage numbers in DIO mice. In addition, (E X -4) 2 -Fc treatment resulted in proinflammatory M1-type macrophages beginning to transform into anti-inflammatory M2-type macrophages. The inflammatory mitogen-activated protein kinase (MAPK) signalling pathway and nuclear factor kappa B (NF-κB) were altered in adipose tissue after (E X -4) 2 -Fc treatment. Leptin has been proven to be closely related to immunity, and we demonstrated that the effect of (E X -4) 2 -Fc on adipocyte inflammation was related to leptin. The data suggested that (E X -4) 2 -Fc could modulate the inflammatory response by inhibiting the expression of leptin in adipose tissue. F0B7 (Ex-4) 2 -Fc could profoundly ameliorate obesity-induced inflammation in the adipose tissues of the DIO mice. F0B7 (Ex-4) 2 -Fc promotes the polarization of M2 macrophages in adipose tissue. F0B7 (Ex-4) 2 -Fc modulates the MAPK and NF-κB signalling pathways in adipose tissue. F0B7 (E X -4) 2 -Fc suppresses obesity-related inflammation by inhibiting leptin secretion. [ABSTRACT FROM AUTHOR]

Komatiites are presented as direct evidence for higher mantle temperatures during the Archean. In the North China Craton, komatiites with spinifex structure have been identified only at one locality, i.e. the Sujiagou area, western Shandong. They were considered as formed during the early Neoarchean mainly based on their association with supracrustal rocks considered to be that age. This study carried out SHRIMP U‐Pb zircon dating on metamorphosed trondhjemitic and monzogranitic dykes intruding the Sujiagou komatiites, and they have magmatic zircon ages of 2592 ± 12 Ma and 2586 ± 13 Ma respectively. This provides direct evidence that the komatiites formed during the early Neoarchean. [ABSTRACT FROM AUTHOR]

Well-crystallized CeO2 cubes of 10–20 nm, which predominantly exposed the polar {100} facet, were fabricated under hydrothermal conditions. The growth mechanism of the nanocubes was investigated by analyzing the structures of the intermediate products at different intervals, and found that the formation route of the nanocubes involved both oriented attachment at the early stage and the Ostwald ripening over the entire process. Ce(OH)3 nanorods were readily formed at the initial stage, while their dissolution, recrystallization and oxidation, under hydrothermal conditions, yielded CeO2 polyhedra, which were further transformed into CeO2 nanocubes. During this growth process, the nucleation and epitaxial growth of CeO2 nanocubes occurred on the Ce(OH)3 nanorods. Moreover, the OH−/Ce3+ ratio in synthetic solution played a decisive role in tuning the size of ceria nanocubes; increasing the OH−/Ce3+ ratio lowered the size of ceria nanocubes. The catalytic properties of the CeO2 nanocubes for CO oxidation showed a prominent size effect; the conversion of CO increased as the size of the nanocubes reduced. [ABSTRACT FROM AUTHOR]

The North China Craton (NCC) is the largest craton on the Chinese Mainland, with 3.8–3.0 Ga rocks occurring mainly in three areas, namely: Anshan-Benxi (Anben), eastern Hebei, and Xinyang. In addition, 3.0 Ga and 3.1 Ga TTG (tonalite-trondhjemite-granodiorite) rocks have also been identified in north Liaoning and the Bohai Bay Basin, respectively. Detrital and xenocrystic zircons dated at 4.1–3.1 Ga occur more widely in the craton. In Anben, >3.3 Ga rocks are mainly distributed in six complexes, whereas in eastern Hebei, 3.8 Ga TTG rocks and 4.0–3.9 Ga detrital zircons have recently been discovered. Anben is similar to eastern Hebei in terms of the Archean zircon age records, but >3.3 Ga TTG rocks are different in both rock-type and composition. The recent identification of 3.8 Ga granitic xenoliths in young volcanic rock at Xinyang indicates the existence of Eoarchean rocks in the deep crust of the craton. Taking the NCC as a whole, the most significant zircon-forming magmatic events occurred at 3.8 Ga, 3.7–3.65 Ga, 3.45 Ga, 3.3 Ga and 3.1 Ga. The >3.0 Ga TTG rocks are mainly trondhjemite, with their low Sr/Y and La/Yb ratios indicating formation under low- to medium-pressure conditions. These are different from 3.0 to 2.5 Ga TTG rocks in the NCC and 3.8–3.0 Ga TTG rocks in southern West Greenland, which are dominated by tonalite, with some having high Sr/Y and La/Yb ratios. The >3.0 Ga rocks have large variations in whole-rock ε Nd (t) and in-situ zircon ε Hf (t), with many showing negative values. Zircons have δ18O values mainly between 5.0 and 7.0‰, consistent with Hadean and Archean magmatic zircon data worldwide. The diversity of Eoarchean granitoid magmatism across the craton indicates that continental evolution on Earth at that time already displayed complexity and maturity. It is further considered that the NCC may contain several different ancient continental nuclei with long-term formation and evolutionary histories back to the Hadean. [ABSTRACT FROM AUTHOR]

[Display omitted] • A new complex, named as the Hujiamiao Complex, was discovered in the Anshan area, NCC. • Trondhjemitic rocks of different ages from 3.79 to 3.11 Ga have been identified. • It is the first time in Anshan to identify trondhjemitic rocks with relatively high (La/Yb) n ratios. • Magmatic zircons have ε Hf (t) of −12.3 to 1.6, t DM (Hf) and t CC (Hf) ages of 4.1 to 3.6 Ga and 4.3 to 3.7 Ga. • All the Anshan 3.8–3.3 Ga complexes are the relics of the same larger complex in the area. Anshan is located in the northeastern North China Craton and has a long-term geological history back to 3.8 Ga. Four complexes with ages of 3.8–3.1 Ga have been identified previously, namely the Baijiafen, Dongshan, Shengousi and Guodishan complexes. Being similar to the neighbouring Beijiafen Complex, the newly-discovered Hujiamiao Complex extends in a northwest-southeast direction and shows tectonic contacts on one side with the Chengtaigou granite and on the other with the Chengtaigou supracrustal rocks. In the Hujiamiao Complex, trondhjemitic rocks of 3789 Ma, 3786 Ma, 3698 Ma, 3617 Ma, 3566 Ma, 3504 Ma and 3106 Ma have been identified. More work is required to establish if a gabbro also has a formation age of 3.8 Ga. The trondhjemitic rocks show similar compositional features in being high in SiO 2 (72.37–76.71 %) and Na 2 O (5.00–6.47 %) and low in K 2 O (0.60–1.95 %), ∑REE contents (32.83–91.83 ppm) and (La/Yb) n ratios (11.09–48.64). They have whole-rock ε Nd (t) values of 0.35–4.43, t DM (Nd) of 3.74–3.46 Ga and t CC (Nd) ages of 3.74–3.43 Ga, and in situ magmatic zircon ε Hf (t) values of −12.3 to 1.56, t DM (Hf) ages of 4.2 to 3.5 Ga and t CC (Hf) ages of 4.6 to 3.7 Ga. Combined with previous work, it is concluded that all the Anshan area 3.8–3.3 Ga complexes are the relics of a larger complex which was once more widely distributed in the Anshan area. The trondhjemitic rather than tonalitic character of the Anshan Eoarchean rocks shows diversity in the formation and evolution of the earliest continental crust with a range of T/P for crustal anatexis. [ABSTRACT FROM AUTHOR]

Abundant mafic‐ultramafic blocks and dikes occur in the area north of Zunhua City, eastern Hebei Province, and were previously suggested to be part of a late Archean ophiolitic assemblage. We employed SHRIMP zircon dating and a geochemical study on these mafic and surrounding rocks to test the ophiolite hypothesis. The SHRIMP data suggest that three metagabbro samples were metamorphosed at ∼1.8 Ga. Numerous ∼2.5 Ga zircons display strong oscillatory zoning, characteristic of zircons from granitoid rocks but not from gabbro, so we suggest that these are xenocrystic grains. The age of these xenocrystic zircons and their metamorphic rims suggests that these mafic blocks formed in Paleoproterozoic. The surrounding gneiss of intermediate composition also contains 2.5 Ga zircons with oscillatory zoning and 1.8 Ga metamorphic rims. Fractionated REE patterns and Nb, Ta, Zr, Hf negative anomalies to variable extent were observed in the mafic blocks and surrounding rocks, also supporting a significant difference in the chemistry of ophiolitic rocks. Our data suggest that many mafic blocks in northern Zunhua are not part of a late Archean ophiolite complex but part of a tectonically dismembered Paleoproterozoic intrusive gabbro complex. This study shows that late Paleoproterozoic metamorphism occurred in the western part of eastern Hebei Province. [ABSTRACT FROM AUTHOR]

Mesoarchean and older potassic granites are important indicators of recycling of ancient continental crust early in Earth's history. This study of integrated whole rock and zircon geochemistry and geochronology reports the age and identification of the source materials of the > 200 km 2 Mesoarchean Tiejiashan-Gongchangling granite in the Anshan-Benxi area, North China Craton, the largest pre-Neoarchean granite domain in the craton. SHRIMP U-Pb zircon dating on 15 samples indicates the magmatic crystallization of the granites between 2.95 and 3.0 Ga and reveals a superimposed tectonothermal event at ~ 2.91 Ga. The granites are characterized by high SiO 2 and K 2 O, low CaO, FeOt, MgO and TiO 2 with peraluminuous features. They show large variations in (La/Yb) n and strong negative Eu and Ba anomalies and Nb, P and Ti depletions. Whole rock Nd and magmatic zircon Hf isotopic compositions show large variations, but with most having ε Nd (t) and ε Hf (t) values < 0, with t DM (Nd) and t DM (Hf) values varying from 3.3 to 3.9 Ga and 3.3 to 4.0 Ga, respectively. Magmatic zircons without very strong lead loss (discordance ≤ 20%) have δ 18 O values of + 3.14 to + 8.39. 3.3–3.7 Ga xenocrystic zircons occur in some samples. The granite formed as a result of recycling of Paleo- to Eoarchean continental material in an intracontinental environment, with little if any contribution from Mesoarchean mantle sources. The sources could be predominantly unaltered ancient gneisses, together with yet to be identified Paleo- to Eoarchean materials affected by early low temperature alteration (weathered rocks or clastic sediment). [ABSTRACT FROM AUTHOR]

There are different viewpoints on metamorphic and anatectic zircons recording ages of 2.45–2.48 Ga or even younger in some areas of the North China Craton where both late Neoarchean and late Paleoproterozoic tectono-thermal events are well developed. These are: 1) partial resetting of the U-Pb isotopic system in the late Neoarchean zircons, 2) metamorphism lasting from the late Neoarchean to the earliest Paleoproterozoic, and 3) earliest Paleoproterozoic metamorphism as separate different event. Western Shandong Province is an area where the late Neoarchean tectono-thermal event is widely developed but the late Paleoproterozoic event has not been identified. This provides an opportunity to understand the geological processes around the Archean-Proterozoic boundary. Based on a field study, we carried out SHRIMP U-Pb zircon dating on seventeen samples of ∼2.5 Ga old metamorphic and anatectic rocks, including tonalite, trondhjemite, granodiorite, gabbro, quartz diorite, granite and paragneiss with primary emplacement or depositional ages of 2.52–2.68 Ga. Anatectic zircons show some textural and compositional features: a) Homogenous or blurred oscillatory zoning, b) high U contents and low in Th/U ratios (commonly <0.3), c) rare idiomorphic morphologies, and d) commonly containing inherited (xenocrystic) cores. We conclude that the strong late Neoarchean event is widespread in western Shandong and is limited between ∼2.50 and 2.54 Ga. In contrast, apparent 2.45–2.48 Ga metamorphic zircon ages in some other areas of the North China Craton may be the result of overprinting and partial recrystallization of Neoarchean metamorphic zircons during the late Paleoproterozoic event. The Archaean rocks of western Shandong can be divided into three belts, namely Belts A, B and C from the northeast to the southwest. The difference between Belts A and B in ∼2.5 Ga metamorphic and anatectic intensity may indicate that the former was elevated to a higher crustal level compared with the latter at the end of the Neoarchean. [ABSTRACT FROM AUTHOR]

The accretion and recycling of Archean-Paleoproterozoic continental crust is the key to understanding the formation and evolution of early continental crust. Early Precambrian metamorphic basement is well developed in the Aktashtagh area, southeastern margin of the Tarim Craton, northwestern China. We report zircon SHRIMP U Pb dating, LA-MC-ICP-MS Lu Hf isotopic analysis and whole-rock geochemical analysis on 16 samples (mainly TTG, tonalite-trondhjemite-granodiorite) from Aktashtagh. Multiple episodes of early Precambrain magmatism, including 3.72 Ga, 3.63 Ga, 3.10 Ga, 2.83–2.69 Ga, 2.55–2.50 Ga, 2.05–2.02 Ga and 1.93–1.82 Ga events, have been identified in the Aktashtagh region. The most intense magmatism occurred in the late Mesoarchean to early Neoarchean. Significantly, 3.63 Ga and 3.10 Ga TTG rocks are identified, together with more extensive 2.8–2.7 Ga and ∼ 2.5 Ga TTG rocks. The TTG suites are dominated by tonalitic, with some trondhjemitic and grandioritic rocks. They show large variations in REE contents, are mainly distributed in the high- and medium-pressure fields in the Sr/Y-Y and La/Yb-Yb diagrams, and the 2.7 Ga TTG rocks commonly show higher and more variable Sr/Y and La/Yb ratios. The 3.63–3.10 Ga TTG rocks have magmatic zircon ε Hf (t) values of −3.9 to +3.1 and t DM1 (Hf) model ages of 3.9–3.6 Ga, whereas the ∼2.7–2.51 Ga TTG rocks have magmatic zircon ε Hf (t) values of −13.4 to +2.1 and t DM1 (Hf) model ages of 3.3–3.0 Ga. The ∼2.7 to ∼2.0 Ga meta-gabbroic-dioritic rocks have magmatic zircon ε Hf (t) values of −19.7 to +0.6 and t DM1 (Hf) model ages of 3.2–2.4 Ga. Metamorphic zircon ages of 3.56 Ga, 2.75–2.73 Ga, 2.53–2.51 Ga, 2.03–1.96 Ga and ∼ 1.85 Ga are also recorded, whereas ∼2.83 Ga, 2.53–2.40 Ga, 2.05–2.02 Ga and 1.86–1.83 Ga crustally-derived K-rich granites are identified, with the most important occurring in the late Neoarchean and late Paleoproterozoic. In this regard, the Tarim Craton is similar to the North China Craton in its early Precambrian geological evolution. The main difference is that the Paleoproterozoic tectonothermal event in the Tarim Craton started as early as 2.00 Ga, whereas in the North China Craton it occurred between 1.95 and 1.80 Ga. • Aktashtagh of the southeastern Tarim Craton has multiple episodes of magmatism from 3.72 to 1.82 Ga. • TTG show large Sr/Y-Y and La/Yb-Yb variations and magmatic zircons have ε Hf (t) of −15.2 to +3.1. • There were multiple Paleoarchaean to Paleoproterozoic events leading to crustal recycling. • The early Precambrian geological records are similar to those in the North China Craton. • The Tarim and North China cratons may be different portions of a larger craton. [ABSTRACT FROM AUTHOR]

[Display omitted] • The Lower Wulashan Group in Daqingshan, northwestern NCC records magmatic zircon ages of 2455–2382 Ma. • Intrusive rocks intruding the Lower Wulashan Group have magmatic zircon ages of 2436–2362 Ma. • It is for the first time to identify early Paleoproterozoic supracrustal rocks in the area. • The Lower Wulashan Group and related intrusive rocks formed in a continental environment. • Three main magmatic episodes of the late Neoarchean to late Paleoproterozoic have been identified. Early Paleoproterozoic supracrustal rocks are relatively rare worldwide and only a few have been reported in the North China Craton (NCC). Daqingshan is a typical area of the Khondalite Belt, northwestern NCC, where early Precambrian supracrustal and intrusive rocks are widely distributed, although the precise age of all units remains unconfirmed. The Lower Wulashan Group is mainly composed of acidic to mafic volcanic and volcano-sedimentary rocks that have undergone amphibolite to granulite facies metamorphism; it was previously considered to be of Mesoarchean age. This study utilized integrated field and petrographic work, whole-rock geochemistry, and Nd whole-rock and zircon U-Pb-Hf isotopic analyses on the Lower Wulashan Group and related intrusive rocks. SHRIMP U-Pb dating of eight samples from the supracrustal rocks, interpreted as metavolcanics, yielded magmatic zircon ages of 2455–2382 Ma, thus for the first time identifying early Paleoproterozoic supracrustal rocks in the Daqingshan area. Thus it is of great importance for understanding the Precambrian evolution of the NCC as well as the Earth because this is within the period when magmatism was globally weak. Six samples from a variety of intrusive rocks have zircon U-Pb ages of 2436–2362 Ma, thus further confirming the depositional ages of the supracrustal rocks. These rocks include meta-alkali basalt, and are high in total rare earth element contents and have magmatic zircon ε Hf (t) values of −3.00 to +6.62 and crustal model ages of 3.12–2.52 Ga (38 analyses), and whole-rock ε Nd (t) of +1.0 to +2.6 with crustal model ages of 2.74–2.60 Ga (5 analyses). It is considered that the early Paleoproterozoic supracrustal rocks of the Lower Wulashan Group and related intrusive rocks formed in a continental environment, but more work is required to determine the tectonic regime (extension or subduction). [ABSTRACT FROM AUTHOR]

[Display omitted] • Green supported of Cu NPs on Fe 3 O 4 nanoparticles using chitosan-starch composite. • The chitosan-starch composite acted as green reductant and stabilizing agent. • The Fe 3 O 4 @CS-Starch/Cu shown good anti-ovarian cancer activity. • The Fe 3 O 4 @CS-Starch/Cu shown good anti-oxidant activity. In the recent days, conjugated bio-nanomaterials involving magnetic core are interested as promising platform in new generation biological composites. In this regard, we report herein a novel Cu NPs supported chitosan-starch composite functionalized Fe 3 O 4 , a dual core–shell type nanoparticle (Fe 3 O 4 @CS-Starch/Cu). The post-synthetically modified biogenic material was analyzed in details over a number of physicochemical methods like, Transmission Electron Microscope (TEM), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray Diffraction (XRD), energy-dispersive X-ray analysis (EDX), vibrating sample magnetometer (VSM) and inductively coupled plasma (ICP) techniques. In the medicinal part, the anticancer properties of Fe 3 O 4 @CS-Starch/Cu against ovarian cancer cell lines were determined. The free radical for the antioxidant effects was DPPH. The IC 50 were 198 and 155 µg/ml against Fe 3 O 4 @CS-Starch/Cu bio-nanocomposite and BHT. The IC 50 of the Fe 3 O 4 @CS-Starch/Cu bio-nanocomposite were 248, 189, and 281 µg/mL against ovarian cancer (PA-1, SW-626, and SK-OV-3) cell lines. In addition, the current study offer that Fe 3 O 4 @CS-Starch/Cu bio-nanocomposite could be a new potential adjuvant chemopreventive and chemotherapeutic agent against cytotoxic cells. [ABSTRACT FROM AUTHOR]

Western Shandong Province is a typical area of the North China Craton (NCC) where Neoarchean plutonic and supracrustal rocks are widely distributed. Early studies documented that ∼2.7 Ga and ∼2.5 Ga magmato-tectono-thermal events are well developed in the area. Here we report SHRIMP U–Pb ages and Hf-in-zircon isotopic data from ten samples of different magmatic rock types including hornblendite, gneissic tonalite, gneissic trondhjemite and gneissic granite. Magmatic zircon grains have 207 Pb/ 206 Pb ages ranging from 2667 to 2598 Ma. Some rocks contain ∼2.5 Ga metamorphic rims and ∼2.7 Ga zircon cores. The magmatic zircon grains have ɛ Hf ( t ) values and Hf crustal model ages of −1.1 to +11.3 and 3.02–2.4 Ga, respectively. Combined with an earlier study, our main conclusions are that the middle Neoarchean rocks mainly occur together with early Neoarchean rocks in the northeastern portion of the central belt. Juvenile additions to continental crust and crustal recycling played important roles in the middle Neoarchean of western Shandong Province, and the entire Neoarchean tectonic evolution can be divided into middle to early Neoarchean (2.75–2.6 Ga) and late Neoarchean (2.6–2.5 Ga) events. [ABSTRACT FROM AUTHOR]

The Daqingshan area in the North China Craton (NCC) incorporates several suites of magmatic intrusives including gabbros, granodiorites, charnockites and syenogranites, which were overprinted by subsequent metamorphic events. In this study, we report detailed morphological and textural characteristics as well as sensitive high resolution ion microprobe (SHRIMP) U-Pb ages of zircons from ten representative samples of the magmatic suites including syenogranites, charnockites and granodiorites. The zircons in these rocks are characterized by typical magmatic crystallization features including elongate prismatic grains with angular to sub-angular faces which are overprinted by a variety of reworked textures indicating strong recrystallization. These structures are terminated by thin and variable metamorphic overgrowths. U-Pb analyses of the zircons reveal multiple tectono-thermal events including magmatic crystallization at 2.30-2.00, 1.96-1.94 and 1.85-1.82 Ga and metamorphic overprint at 1.97-1.94 and 1.87-1.82 Ga. The episodic magmatism and regional metamorphism recorded in our study show a complex sequence of tectonic processes during the early to late Palaeoproterozoic associated with the amalgamation of crustal blocks within the NCC, with the youngest episode of the syenogranites representing regional extension at the end of the final cratonization of the NCC. Copyright © 2012 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Abstract: At the end of the Neoarchean continental evolution, voluminous syenogranites were emplaced in the North China Craton, together with other magmatic rocks (trondhjemite–tonalite–granodiorite (TTG), monzogranite, diorite, gabbro). Syenogranites are widely distributed in Anshan-Benxi, Qinhuangdao and western Shandong, and also occur in southern Jilin, northern Liaoning, northwestern Hebei and central Henan. Based on geological relationships, degree of metamorphism, deformation and magmatic zircon ages, two phases of syenogranite magmatism are recognized. Rocks produced during the first phase show a gneissic texture and were formed between 2.53 and 2.52Ga and locally comprise abundant TTG. Rocks of the second phase cut late Neoarchean TTG and supracrustal rocks, display a massive structure, and mainly formed between 2.52 and 2.50Ga. All syenogranites share the same features in major element compositions, being high in SiO2 and low in CaO, total FeO, MgO, TiO2 and P2O5. However, they are different in trace and REE compositions and can be subdivided into three types. (1) Type 1 shows a large variation in total REE contents, low (La/Yb) n ratios, strong negative Eu*/Eu anomalies and Ba depletion; (2) Type 2 is similar to Type 1 but has higher (La/Yb) n ratios. (3) Type 3 shows a large variation in total REE and (La/Yb) n ratios and significantly do not show strongly negative Eu*/Eu anomalies and Ba depletion. Whole-rock Sm–Nd isotopic compositions show large variations in ɛ Nd(t) values and t DM(Nd) modal ages, ranging from −9.49 to −4.72 and 3.70 to 3.25Ga (Type 1), 0.55–1.03 and 2.77–2.71Ga (Type 2) and −2.35 to 1.23 and 2.93–2.66Ga (Type 3), respectively. Hf isotopic compositions of zircons from three samples have ɛ Hf(t) values and t DM1(Hf) ages of 0.7–7.2 and 2.84–2.56Ga (Type 1), 2.6–7.4 and 2.74–2.56Ga (Type 2) and 2.1–6.3 and 2.76–2.60Ga (Type 3). It is concluded that syenogranites were generated by melting of continental crust with different mean crustal residence ages, and most of them were emplaced during the second phase (2.52–2.50Ga) in an extensional tectonic regime. The formation of these voluminous syenogranites marks a tectono-magmatic event resulting in stabilization of the North China Craton at the end of the Neoarchean. [Copyright &y& Elsevier]

A dual stimulus-responsive mPEG-SS-PLL15-glutaraldehydestar (mPEG-SS-PLL15-star) catiomer is developed and biologicallyevaluated. The catiomer system combines redox-sensitive removal ofan external PEG shell with acid-induced escape from the endosomalcompartment. The design rationale for PEG shell removal is to augmentintracellular uptake of mPEG-SS-PLL15-star/DNA complexesin the presence of tumor-relevant glutathione (GSH) concentration,while the acid-induced dissociation is to accelerate the release ofgenetic payload following successful internalization into targetedcells. Size alterations of complexes in the presence of 10 mM GSHsuggest stimulus-induced shedding of external PEG layers under redoxconditions that intracellularly present in the tumor microenvironment.Dynamic laser light scattering experiments under endosomal pH conditionsshow rapid destabilization of mPEG-SS-PLL15-star/DNA complexesthat is followed by facilitating efficient release of encapsulatedDNA, as demonstrated by agarose gel electrophoresis. Biological efficacyassessment using pEGFP-C1 plasmid DNA encoding green fluorescenceprotein and pGL-3 plasmid DNA encoding luciferase as reporter genesindicate comparable transfection efficiency of 293T cells of the catiomerwith a conventional polyethyleneimine (bPEI-25k)-based gene deliverysystem. These experimental results show that mPEG-SS-PLL15-star represents a promising design for future nonviral gene deliveryapplications with high DNA binding ability, low cytotoxicity, andhigh transfection efficiency. [ABSTRACT FROM AUTHOR]

Early to Middle Ordovician strata, including Wenquan quartzite, occur widely in the Himalaya, Lhasa, and south Qiangtang blocks. The Wenquan quartzite occurs on the south side of the Lungmuco-Shuanghu Suture in the Qiangtang area, Tibet. A total of 145 analyses on detrital zircons from the quartzite show five age ranges of 520-700, ca. 800, 900-1100, 1800-1900, and 2400-2500 Ma, with particularly distinct age peaks of 625 and 950 Ma. The reliable youngest detrital zircon age is 525 Ma, and the oldest, 3180 Ma. Detrital zircons show large variations in Hf isotope composition, with depleted mantle model ages t(Hf) ranging from 750 to 3786 Ma. Based on data obtained in this study and by others, the main conclusions are as follows: 1) Low-grade metamorphic sedimentary rocks are distributed extensively in the south of the Lungmuco-Shuanghu Suture and are Phanerozoic in age; 2) Pan-African and Grenville-Jinning tectono-thermal events were well developed in the source region of the Wenquan quartzite; 3) the source region shows crustal addition and recycling of different periods; 4) Wenquan quartzite was derived from the Gondwana metamorphic basement, suggesting that the Qiangtang block is a Gondwanan fragment. [ABSTRACT FROM AUTHOR]

Zircom U-Pb age and Hf isotope analyses were made on gneissic granite and garnet-mica two-feldspar gneiss from the Helanshan Group in the Bayan Ul-Helan Mountains area, the western block of the North China Craton (NCC). Zircons from the gneissic granite commonly show core-mantle-rim structures, with magmatic core, metamorphic mantle and rim having ages of 2323±20 Ma, 1923±28 Ma and 1856±12 Ma, respectively. The core, mantle and rim show similar Hf isotope compositions, with single-stage depleted mantle model ages (TDM1) of 2455 to 2655 Ma (19 analyses). Most of the detrital zircons from the garnet-mica two-feldspar paragneiss have a concentrated U-Pb age distribution, with a weighted mean 207Pb/206Pb age of 1978±17 Ma. A few detrital zircons are older (2871 to 2469 Ma). The age for metamorphic overgrown rim was not determined because of strong Pb loss due to their high U content. The zircons show large variation in Hf isotope composition, with TDM1 ages of 1999 to 3047 Ma. In combination with previous studies, the main conclusions are as follows: (1) protolith of the khondalite series in the Helanshan Group formed during Palaeoproterozoic rather than the Archaean as previously considered; (2) The results lend support to the contention that there is a huge Palaeoproterozoic Khondalite (metasedimentary) Belt between the Yinshan Mountains Block and the Ordos Block in the Western Block of NCC; (3) The widely-distributed bodies of early Palaeoproterozoic orthogneisses in the Khondalite Belt might be one of the important sources for detritus material in the khondalite series; (4) Collision between the Yinshan Block, the Ordos Block and the Eastern Block occurred in the same tectonothermal event of late Palaeoproterozoic, resulting in the final assembly of the NCC. [ABSTRACT FROM AUTHOR]

Western Shandong is the only area in the North China Craton where abundant supracrustal rocks of the early and late Neoarchean are developed. This study presents an integrated geological investigations, whole-rock geochemistry and SHRIMP U Pb zircon dating investigation of the supracrustal rocks at Yanlingguang, one of the most typical areas of Neoarchean supracrustal rocks in western Shandong. The original Yanlingguan 'formation' is now subdivided into three belts: the Northeastern, Central and Southwestern belts, which are all in tectonic contact. The Northeastern and Southwestern belts are mainly composed of meta-komatiitic and basaltic rocks. Four granitic dykes intruding these belts have magmatic zircon ages of 2731–2530 Ma, supporting the conclusion that the supracrustal rocks were formed in the early Neoarchean. Meta-basalt with pillow structures has been identified in several locations, with the largest being ~35 m in thickness. The meta-komatiitic rocks are Al-undepleted komatiites and have ∑REE and (La/Yb) n of 2.30–26.21 ppm and 0.29–2.35, respectively, whereas the meta-basaltic rocks have ∑REE and (La/Yb) n of 16.45–53.26 ppm and 0.67–1.78, respectively, and ε Nd (t) values of 3.36–4.76, being similar to N-MORB. Eleven samples of meta-sedimentary rocks (including meta-sandstones-siltstones and meta-conglomerates) from the Central Belt have zircon U Pb ages extending from 2760 to 2520 Ma, providing evidence for the first time that they were deposited during the late Neoarchean, rather than the early Neoarchean as previously thought. The meta-sandstones-siltstones show large variations in major element contents, but have low ∑REE (49.17–102.30 ppm) and (La/Yb) n (1.53–7.00) ratios, with negligible to weakly negative or positive Eu anomalies (Eu/Eu* = 0.60–1.17), and record ε Nd (t) values of 0.96–3.21. Combined with regional geological investigations, we conclude that the early Neoarchean supracrustal rocks were formed in an oceanic environment. It is also suggested that the sandstones-siltstones of the late Neoarchean were derived from the early Neoarchean basement, and that the conglomerates were derived from two separate source regions; one of early, and the other of late Neoarchean age. The identification of basal conglomerates is consistent with the arc model established in early studies. We suggest that the criteria employed in this study can be used for determining whether or not an Archean greenstone belt is of oceanic affinity. • Yanlingguang in western Shandong contains three greenstone belts that differ in age. • The Northwestern and Southwestern belts are early Neoarchean. • The Central belt is late Neoarchean. • Early Neoarchean ultramafic and mafic rocks formed at 2.75 Ga in an oceanic setting. • Late Neoarchean metasedimentary rocks formed at 2.52 Ga in an island arc setting. [ABSTRACT FROM AUTHOR]

Despite drug delivery nanoplatforms receiving extensive attention, development of a simple, effective, and multifunctional theranostics nanoplatform still remains a challenge. Herein, a versatile nanoplatform based on a zirconium framework (UiO‐66‐N3) was synthesized, which demonstrated a combined photodynamic therapy (PDT), photothermal therapy (PTT), and chemotherapy (CT) for cancer treatment. A RuII polypyridyl alkyne complex (Ra) as a photosensitizer was modified into a nanoplatform by click reactions for the first time. When exposed to suitable light irradiation, the as‐prepared multifunctional nanoplatform (UiO‐Ra‐DOX‐CuS) not only demonstrated efficient 1O2 generation, but also exhibited excellent photothermal conversion ability. In particular, the nanotherapeutic agent presented a dual‐stimuli response; either acidic environment or NIR laser irradiation would trigger the drug release. The synergetic efficacy of UiO‐Ra‐DOX‐CuS combined PDT, PTT, and CT, which was evaluated by cell experiments. Moreover, the design could promote the development of RuII polypyridyl alkyne complexes based multifunctional nanoparticles and multimodal cancer treatment. [ABSTRACT FROM AUTHOR]

Background & Aims: In liver cancer, leucine‐rich repeat‐containing G‐protein coupled receptor 5 (LGR5) compartment represents an important tumor‐initiating cell (TIC) population and served as a potential therapeutic target. Cancer‐associated fibroblasts (CAFs) is a critical part of the tumor microenvironment, heavily influenced TIC function and fate. However, deeply investigations have been hindered by the lack of accurate preclinical models to investigate the interaction between CAFs and TIC. Organoids model have achieved major advancements as a precious research model for recapitulating the morphological aspects of organs, and thus also serving as a candidate model to investigate the mutual interaction between different cell types. Consequently, this study aimed to construct a three‐dimensional (3D) co‐culture organoid model of primary LGR5‐expressing tumor stem cells from primary murine liver tumors with CAFs to investigate the impact of CAFs on LGR5 marked TICs in liver cancer. Materials and Methods: First, both of the transgenic LGR5‐diphtheria toxin receptor (DTR)‐GFP knock‐in mice and transgenic Rosa26‐mT mice developed primary liver tumors by diethylnitrosamine (DEN) administration. Tumor organoids and CAFs were generated from those primary liver cancer separately. Second, LGR5‐expressing TICs organoid with CAFs were established ex vivo based on cell–cell contact or trans‐well co‐culture system, and the mutual influence between those two types of cells was further investigated. Subsequently, immunodeficient mouse‐based xenograft model was further adopted to evaluate the influence of CAFs to LGR5 tumor stem cell, tumor formation, and metastasis. Results: The co‐culture organoid model composed of murine liver tumor LGR5+ tumor‐initiating cells and CAFs in 3D co‐culture was successfully established, with the intention to investigate their mutual interaction. The existence of CAFs upon engrafting tumor organoids resulted in dramatic higher number of LGR5+ cells in the neoplasia when compared with engrafting tumor organoids alone. Furthermore, ex vivo culture of isolated LGR5+ cells from tumors of co‐engrafted mice formed significantly larger size of organoids than mono‐engrafted. Our results also indicated significantly larger size and number of formed organoids, when LGR5+ cells co‐cultured with CAF in both cell–cell contact and paracrine signaling in vitro, comparing to LGR5+ cells alone. Furthermore, we found that specific knockout of LGR5 expressing cells suppressed CAF‐mediated promotion of tumor formation, growth, and metastasis in the experimental mice model. Conclusions: Altogether, in a 3D co‐culture type of murine liver LGR5+ cells and cancer‐associated fibroblasts, we have demonstrated robust effects of CAFs in the promotion of LGR5 marked liver TICs. We also further revealed the influence of tumor microenvironment on stem cell‐related therapy, suggesting the possibility of combing CAF‐targeted and tumor stem cell targeted therapy in treating liver cancer. [ABSTRACT FROM AUTHOR]

• Copper-related agents for cancer therapy are overviewed. • Copper depletion for the inhibition of tumor proliferation are summarized. • A series of copper suppliers for tumor removal are reviewed. • Challenges and developments of Cu-related anticancer agents are discussed. As an indispensable micronutrient, copper (Cu) plays an important role in promoting metabolism and maintaining various basic biological functions. Copper homeostasis in the human body is tightly regulated and the imbalance of copper levels can lead to metabolic abnormalities and toxic effects on cells. Hence, Cu-related agents have sparked tremendous attention in the field of cancer therapy. Two copper-related strategies have been proposed for the treatment of tumors: depleting copper inside tumors to inhibit proliferation, and providing excess copper to promote cell apoptosis or cuproptosis. Correspondingly, copper depletors are adopted to reduce copper bioavailability, and a variety of copper-based agents, including copper-based nanosystems, copper complexes and nano-copper complexes, are applied to supplement copper. This review systematically summarizes the recent development of Cu-related agents for cancer therapies from the perspective of copper depletion and copper supplementation. Considering the large number of agents used to supply copper, copper-based nanosystems, copper complexes and nano-copper complexes are discussed in separate sections. Finally, the challenges and the future developments of Cu-related anticancer agents are explored to provide some enlightenment for future research. [ABSTRACT FROM AUTHOR]

The clinical efficacy of personalized cancer vaccines still needs to be improved due to their insufficient immune effect. The development of innovative adjuvants and lymph node‐targeted delivery systems is the key to improving the clinical efficacy of personalized vaccines. However, there is still a lack of an adjuvant delivery system that is simple in preparation and capable of mass production and integrates adjuvant and lymph node targeted delivery functions. Here, this work reports that a simple dendrimer polypeptide (KK2DP7) nanoparticle enhances the immune efficacy of an OVA/neoantigen‐based vaccine. Due to its multiple functions as a delivery vehicle, immune adjuvant, and facilitator of dendritic cell migration, KK2DP7 efficiently increases the efficiency of antigen uptake and cross‐presentation by antigen‐presenting cells (APCs) and delivers antigens to lymph nodes via APCs. Strikingly, the antitumor effect of KK2DP7/OVA is superior to that of commonly used adjuvants such as poly(I:C), CpG, and aluminum adjuvant combined with OVA. Furthermore, KK2DP7/OVA combined with anti‐PD‐1 antibody is able to prevent tumor recurrence in a postoperative recurrent tumor model. Thus, KK2DP7‐based cancer vaccines alone or in combination with immune checkpoint blockade therapies to treat tumors or postoperative tumor recurrence are a powerful strategy to enhance antitumor immunity. [ABSTRACT FROM AUTHOR]

Human bone marrow-derived mesenchymal stem cells (hBMSCs) are promising candidates for stem cell therapy in clinical trials. Applications of hBMSCs in clinical therapy are limited by cellular senescence due to long-term ex vivo expansion. Metformin, an oral hypoglycemic drug for type 2 diabetes, has been shown to have antiaging effects. However, the mechanisms of metformin in antiaging treatment remain controversial. Here, we used D-galactose (D-gal) to establish an appropriate model of senescent hBMSCs to explore the antiaging effects of metformin. Following metformin treatment with a low concentration range, senescence phenotypes induced by D-gal significantly changed, including generation of reactive oxygen species (ROS), loss of mitochondrial membrane potential (MMP), and cell cycle arrest. In contrast, no apparent change was found in unsenescent hBMSCs. Furthermore, the results show that activation of 5 ′ AMP-activated protein kinase (AMPK) by metformin enhances cell autophagy in senescent hBMSCs. These findings suggest that metformin exerts antiaging function within the low concentration range by enhancing autophagy and exhibits potential benefits for clinical stem cell therapy by ameliorating the ex vivo replicative senescence of hBMSCs. [ABSTRACT FROM AUTHOR]

The therapeutic application of chemodynamic therapy (CDT) is severely limited by the insufficient intracellular H 2 O 2 and acidity in tumor. Herein, an acid-sensitive nanoplatform (ZIF67-ICG/TAM@GOx) to promote H 2 O 2 and acidity enhancement through intracellular cyclic amplification for enhanced CDT is rationally designed. Notably, the acidic conditions of the tumor microenvironment (TME) can turn on the switch of the nanoplatform, setting free the loaded tamoxifen (TAM) and indocyanine green (ICG). The mitochondrial respiration inhibitor TAM and the superoxide dismutase-mimicking ZIF67 synergistically lead to an increase in the content of O 2 and H 2 O 2 , accelerating the depletion of β- d -glucose by GOx to generate gluconate and H 2 O 2. The gluconate in turn boosts the acidity to facilitate the collapse of nanoparticles, further significantly promoting the accumulation of intracellular H 2 O 2 through a positive circulation. Consequently, the amplificated endogenous H 2 O 2 is catalyzed by Co2+ to liberate hydroxyl radicals (•OH). Besides, ICG-mediated photothermal therapy (PTT) and GOx-induced starvation therapy along with CDT realize the synergistic cancer treatment. Importantly, in vitro and in vivo experiments verified that the nanoplatform performed superior specificity and excellent therapeutic responses. The smart nanoplatform overcomes H 2 O 2 and acidity deficiency simultaneously for intensive CDT, providing new prospects for the development of biocompatible cancer synergistic therapy strategies. In the tumor microenvironment, the released TAM, GOx and ZIF67 synergistically lead to a positive circulation for self-amplified CDT, cooperating with PTT and starvation to realize the synergistic cancer treatment. [Display omitted] • An acidic sensitive nanoplatform (ZIF67-ICG/TAM@GOx) is constructed for specific tumor therapy. • The combination of TAM, ZIF67 and GOx enabled positive circulation to accumulate H 2 O 2 and acidity for boosting CDT. • The disruption of H 2 O 2 homeostasis made the approach of "expanding source and cost-saving" for the increase of H 2 O 2. • The self-amplified CDT along with PTT, GOx-induced starvation realized a novel synergistic cancer therapy strategy. [ABSTRACT FROM AUTHOR]

The 2022 Winter Olympics will be held in the Beijing-Zhangjiakou area, which is located in the eastern North China Craton (NCC) and contains a comprehensive geological record from the Archaean to Cenozoic. In this paper, we first review the geology of the Beijing-Zhangjiakou area, then, combined with data from the rest of the NCC, we discuss the geological evolution of the area in the context of the NCC. The geological and tectonic evolution of the Beijing-Zhangjiakou area can be regarded as a proxy or analogue for the geological history of the whole of the NCC. Six stages have been divided as follows: 1) Neoarchean massive continental crust growth; 2) Paleoproterozoic cratonization; 3) Meso-Neoproterozoic multi-stage rifting; 4) Palaeozoic marginal orogeny; 5) Mesozoic craton destruction; and 6) Cenozoic intra-plate extension. We hope this paper will be helpful to both geologists and non-geologists who will attend the 2022 Beijing-Zhangjiakou Winter Olympics and may be interested in rocks and geology around the Winter Olympic sites. [ABSTRACT FROM AUTHOR]

Although the survival rate of hematological malignancies (HM) has increased in recent years, the unnecessary adverse effect to the body is usually generated by the traditional chemotherapy for HM due to the lack of specificity to tumor tissue. Nanodrug delivery systems have exhibited unique advantages in targetability, stability and reducing toxicity, attracting wide concern, which is expected to be the prevalent alternative for the treatment of HM. In this review, we systemically introduced the current therapeutic strategies and the categories of HM. Subsequently, five key factors including circulation, targeting, penetration, internalization and release involving in tailoring nanoparticles were demonstrated, followed by the introduction of the development of nanodrug delivery—traditional synthetic nanomaterilas, biomimetic cell membrane coating nanomaterials, cell-based nanomaterials as well as immunotherapy combined with nanodrug. Afterwards, the recent advances of nanodrug delivery system for the treatment of HM were introduced. Moreover, the challenge and prospect of nanodrug delivery system in treating HM were discussed. The promising drug delivery system will provide new therapeutic avenues for the treatment of HM. [ABSTRACT FROM AUTHOR]

Although the survival rate of hematological malignancies (HM) has increased in recent years, the unnecessary adverse effect to the body is usually generated by the traditional chemotherapy for HM due to the lack of specificity to tumor tissue. Nanodrug delivery systems have exhibited unique advantages in targetability, stability and reducing toxicity, attracting wide concern, which is expected to be the prevalent alternative for the treatment of HM. In this review, we systemically introduced the current therapeutic strategies and the categories of HM. Subsequently, five key factors including circulation, targeting, penetration, internalization and release involving in tailoring nanoparticles were demonstrated, followed by the introduction of the development of nanodrug delivery—traditional synthetic nanomaterilas, biomimetic cell membrane coating nanomaterials, cell-based nanomaterials as well as immunotherapy combined with nanodrug. Afterwards, the recent advances of nanodrug delivery system for the treatment of HM were introduced. Moreover, the challenge and prospect of nanodrug delivery system in treating HM were discussed. The promising drug delivery system will provide new therapeutic avenues for the treatment of HM. [ABSTRACT FROM AUTHOR]

Since there are several limitations in cancer treatment for traditional chemotherapy, such as side effects, poor prognosis and drug resistance, developing new combined therapy is urgently needed. In this work, a biocompatible, simple and tumor microenvironment-responsive nanotheranostics (PCN-Oxpt/PEG) was built to favor the chemotherapy/ferroptosis/immunomodulation synergism in cancer. This nanotheranostics is constructed by modifying oxaliplatin prodrug and PEG on Fe(III) − porphyrin metal-organic frameworks (PCN(Fe) MOFs). After intravenous injection, the cloak of PEG leads to long circulation, and the Fe(III)-porphyrin MOFs enables dual-model guidance with fluorescence (FL) and magnetic resonance imaging (MRI). Inside the tumor, the intracellular H 2 O 2 would be transferred into hydroxyl radicals (•OH) by iron ions released from MOFs, which could trigger the lethal ferroptosis to cancer cells. Meanwhile, oxaliplatin(II) transformed from the loaded oxaliplatin prodrug would result in the chemotherapy, as well as immunogenic cell death (ICD), and the prodrug strategy could also avoid the occurring of liver damage by the direct administration of oxaliplatin(II). It was noticed that the ferroptosis effect was enhanced by triple-assistance during the combined therapy, as followed: (1) glutathione (GSH) would be consumed in the process of oxaliplatin(II) generation from oxaliplatin prodrug; (2) the increased CD8+ T cells induced by ICD were able to produce interferon-γ (IFN-γ), which could inhibit the transport of cystine by tumor cells, and impair the activation of glutathione peroxidase 4 (GPX4); (3) the amount of H 2 O 2 could be increased by the internalized oxaliplatin and thus further promote the Fenton reaction and ferroptosis. Both in vivo and in vitro results revealed that tumor growing was significantly inhibited by PCN-Oxpt/PEG, taken together, the concomitant of oxaliplatin-mediated chemotherapy and ICD with triple-enhanced ferroptosis offer great prospect in the clinical treatment of cancer. [Display omitted] • A novel nanoplatform (PCN-Oxpt/PEG) was designed for eliminating tumor with combined therapy. • The designed nanoplatform enables dual-model imaging (FL and MRI) to guide antitumor therapy. • Immunosuppressive tumor micro-environment could be remodeled by PCN-Oxpt/PEG, which would enhance ferroptosis efficiently. [ABSTRACT FROM AUTHOR]

Simple Summary: Multidrug resistance (MDR) is a major cause of breast cancer (BC) chemotherapy failure. Long noncoding RNAs (lncRNAs) have been shown closely related to the chemoresistance of BC. In this work, the mechanisms of lncRNA-mediated MDR in BC were elaborated from eight sections, including apoptosis, autophagy, DNA repair, cell cycle, drug efflux, epithelial-mesenchymal transition, epigenetic modification and the tumor microenvironment. Additionally, we also discuss the clinical significance of lncRNAs, which may be biomarkers for diagnosis, therapy and prognosis. Breast cancer (BC) is a highly heterogeneous disease and presents a great threat to female health worldwide. Chemotherapy is one of the predominant strategies for the treatment of BC; however, multidrug resistance (MDR) has seriously affected or hindered the effect of chemotherapy. Recently, a growing number of studies have indicated that lncRNAs play vital and varied roles in BC chemoresistance, including apoptosis, autophagy, DNA repair, cell cycle, drug efflux, epithelial-mesenchymal transition (EMT), epigenetic modification and the tumor microenvironment (TME). Although thousands of lncRNAs have been implicated in the chemoresistance of BC, a systematic review of their regulatory mechanisms remains to be performed. In this review, we systematically summarized the mechanisms of MDR and the functions of lncRNAs mediated in the chemoresistance of BC from the latest literature. These findings significantly enhance the current understanding of lncRNAs and suggest that they may be promising prognostic biomarkers for BC patients receiving chemotherapy, as well as therapeutic targets to prevent or reverse chemoresistance. [ABSTRACT FROM AUTHOR]

As an iron dependent regulatory cell death process driven by excessive lipid peroxides (LPO), ferroptosis is recognized as a powerful weapon for pancreatic cancer (PC) therapy. However, the tumor microenvironment (TME) with hypoxia and elevated glutathione (GSH) expression not only inhibits LPO production, but also induces glutathione peroxidase 4 (GPX4) mediated LPO clearance, which greatly compromise the therapeutic outcomes of ferroptosis. To address these issues, herein, a novel triple-enhanced ferroptosis amplifier (denoted as Zal@HM-PTBC) is rationally designed. After intravenous injection, the overexpressed H 2 O 2 /GSH in TME induces the collapse of Zal@HM-PTBC and triggers the production of oxygen and reactive oxygen species (ROS), which synergistically amplify the degree of lipid peroxidation (broaden sources). Concurrently, GSH consumption because of the degradation of the hollow manganese dioxide (HM) significantly weakens the activity of GPX4, resulting in a decrease in LPO clearance (reduce expenditure). Moreover, the loading and site-directed release of zalcitabine further promotes autophagy-dependent LPO accumulation (enhance effectiveness). Both in vitro and in vivo results validated that the ferroptosis amplifier demonstrated superior specificity and favorable therapeutic responses. Overall, this triple-enhanced LPO accumulation strategy demonstrates the ability to facilitate the efficacy of ferroptosis, injecting vigorous vitality into the treatment of PC. [Display omitted] • Zal@HM-PTBC was designed to facilitate ferroptosis by triple-enhanced LPO accumulation strategy. • TME induced the collapse of Zal@HM-PTBC and triggered the production of O 2 /ROS , which synergistically amplify LPO level. • The degradation of Zal@HM-PTBC consumed GSH in TME and attenuated "detoxification effect" on LPO by inactivating of GPX4. • The release of zalcitabine effectively led to autophagy dependent LPO accumulation. [ABSTRACT FROM AUTHOR]

Abstract: In the Daqingshan area within the Khondalite Belt of the North China Craton, Paleoproterozoic gabbro and dolerite intrusions show amphibolite to granulite-facies metamorphism. U–Pb zircon dating and whole-rock geochemistry were undertaken on these rocks in order to understand the timing of mantle magmatism and thermal processes. Ten samples yielded zircon U–Pb formation ages of 2.46–2.44Ga, 1.97–1.92Ga and 1.84Ga and metamorphic ages of 1.95–1.83Ga. They are variable in geochemical composition with some being enriched in light rare earth and large ion lithosphere elements but depleted in Nb–Ta. Combined with previous results, it is concluded that mafic magmatism at 2.45–2.37Ga and 1.97–1.92Ga indicates two Paleoproterozoic extensional phases, with the latter accompanied by HT–UHT metamorphism as a result of underplating of mantle magma. [Copyright &y& Elsevier]

Background: Chemodynamic therapy (CDT), employing Fenton or Fenton-like catalysts to convert hydrogen peroxide (H2O2) into toxic hydroxyl radicals (·OH) to kill cancer cells, holds great promise in tumor therapy due to its high selectivity. However, the therapeutic effect is significantly limited by insufficient intracellular H2O2 level in tumor cells. Fortunately, β-Lapachone (Lapa) that can exert H2O2-supplementing functionality under the catalysis of nicotinamide adenine dinucleotide (phosphate) NAD(P)H: quinone oxidoreductase-1 (NQO1) enzyme offers a new idea to solve this problem. However, extensive DNA damage caused by high levels of reactive oxygen species can trigger the "hyperactivation" of poly(ADP-ribose) polymerase (PARP), which results in the severe interruption of H2O2 supply and further the reduced efficacy of CDT. Herein, we report a self-amplified nanocatalytic system (ZIF67/Ola/Lapa) to co-deliver the PARP inhibitor Olaparib (Ola) and NQO1-bioactivatable drug Lapa for sustainable H2O2 production and augmented CDT ("1 + 1 + 1 > 3"). Results: The effective inhibition of PARP by Ola can synergize Lapa to enhance H2O2 formation due to the continuous NQO1 redox cycling. In turn, the high levels of H2O2 further react with Co2+ to produce the highly toxic ·OH by Fenton-like reaction, dramatically improving CDT. Both in vitro and in vivo studies demonstrate the excellent antitumor activity of ZIF67/Ola/Lapa in NQO1 overexpressed MDA-MB-231 tumor cells. Importantly, the nanocomposite presents minimal systemic toxicity in normal tissues due to the low NQO1 expression. Conclusions: This design of nanocatalytic system offers a new paradigm for combing PARP inhibitor, NQO1-bioactivatable drug and Fenton-reagents to obtain sustained H2O2 generation for tumor-specific self-amplified CDT. [ABSTRACT FROM AUTHOR]

Herein, a multi-functional nanoplatform (PDA-DTC/Cu–MnO2) was established, which has been employed for MR imaging-guided multi-therapy (CDT, PTT and chemotherapy) for cancer treatment. The in vitro and in vivo results confirmed that the biocompatible nanoplatform could significantly induce tumor cell death and inhibit tumor growth. [ABSTRACT FROM AUTHOR]

Background: Antimicrobial peptides are promising alternative antimicrobial agents to combat MDR. DP7, an antimicrobial peptide designed in silico, possesses broad-spectrum antimicrobial activities and immunomodulatory effects. However, the effects of DP7 against Pseudomonas aeruginosa and biofilm infection remain largely unexplored.Objectives: To assess (i) the antimicrobial activity of DP7 against MDR P. aeruginosa; and (ii) the antibiofilm activity against biofilm infection. Also, to preliminarily investigate the possible antimicrobial mode of action.Methods: The MICs of DP7 for 104 clinical P. aeruginosa strains (including 57 MDR strains) and the antibiofilm activity were determined. RNA-Seq, genome sequencing and cell morphology were conducted. Both acute and chronic biofilm infection mouse models were established. Two mutants, resulting from point mutations associated with LPS and biofilms, were constructed to investigate the potential mode of action.Results: DP7, at 8-32 mg/L, inhibited the growth of clinical P. aeruginosa strains and, at 64 mg/L, reduced biofilm formation by 43% to 68% in vitro. In acute lung infection, 0.5 mg/kg DP7 exhibited a 70% protection rate and reduced bacterial colonization by 50% in chronic infection. DP7 mainly suppressed gene expression involving LPS and outer membrane proteins and disrupted cell wall structure. Genome sequencing of the DP7-resistant strain DP7R revealed four SNPs controlling LPS and biofilm production. gshA44 and wbpJ139 mutants displayed LPS reduction and motility deficiency, conferring the reduction of LPS and biofilm biomass of strain DP7R and indicating that LPS was a potential target of DP7.Conclusions: These results demonstrate that DP7 may hold potential as an effective antimicrobial agent against MDR P. aeruginosa and related infections. [ABSTRACT FROM AUTHOR]

To overcome the acquired resistance and the significant side-effects of the reported drugs, four new ruthenium(II) complexes with alkynyl (Ru1, Ru2, Ru3, Ru4) were designed and synthesized. Ru1, Ru2, Ru3 and Ru4 were characterized by ESI-MS, 1H NMR, 1H–1H COSY NMR and elemental analysis. Compared with Ru2, Ru3, Ru4 and cisplatin, the anti-tumor experiments in vitro and vivo confirmed that Ru1 could most effectively inhibit tumor growth. In the experiments of safety evaluation in vivo, Ru1 could avoid any detectable side-effects compared with cisplatin. DNA binding experiments and cell cycle experiments showed that Ru1 exhibited the strongest DNA binding ability and interfered with the cell cycle by inserting DNA to inhibit tumor growth. The study demonstrated that Ru1 had the potential to be an exciting new drug candidate for glioblastoma treatment. [ABSTRACT FROM AUTHOR]

Despite its great potential in cancer therapy, phototherapy, including photothermal therapy (PTT) and photodynamic therapy (PDT), often cause metastasis of tumors. Immunotherapy has revolutionized the cancer treatment owing to the capability of activating immune system to eliminate tumors. However, the integration of phototherapy and immunotherapy in a single nanoagent for cancer therapy is still a challenging task. Here, we fabricated (Cu9S5@mSiO2-PpIX@MnO2@CpG (CSPM@CpG)) as a synergistic therapeutic model for phototherapy enhanced immunotherapy. The intracellular uptake of cytosine-phosphate-guanine (CpG) promoted the infiltration of cytotoxic T lymphocytes (CTLs) in tumor tissue, further stimulating the production of interferon gamma (IFN-γ) and remarkably elevating the immune response level. Excellent anti-tumor effects have been achieved by synergistic PTT/PDT/immunotherapy. The metastasis of tumors was effectively inhibited by the immune response of CpG. Thus, our proposed work provides a strategy to combine phototherapy with immunotherapy to enhance the therapeutic efficiency and further inhibit metastasis of tumors. CpG oligonucleotides can stimulate an anticancer immune response. Zhou et al. now develop nanoparticles that can deliver CpG, which promotes cancer cell death after photothermal and photodynamic therapy in vitro and in vivo, concomitant with an immunological response. [ABSTRACT FROM AUTHOR]

Based on the Pt nanozyme modified mesoporous polydopamine in situ, a multi-functional nanoplatform was established, which could overcome tumour hypoxia by catalyzing overexpressed H2O2 in tumour cells to enhance photodynamic therapy. In vivo results confirmed that the tumour growth was inhibited efficiently by synergetic therapy. [ABSTRACT FROM AUTHOR]

As a type of nanomaterials with enzyme-mimetic catalytic properties, nanozymes have attracted wide concern in biological detection. H 2 O 2 was the characteristic product of diverse biological reactions, and the quantitative analysis for H 2 O 2 was an important way to detect disease biomarkers, such as acetylcholine, cholesterol, uric acid and glucose. Therefore, there is of great significance for developing a simple and sensitive nanozyme to detect H 2 O 2 and disease biomarkers by combining with corresponding enzyme. In this work, Fe-TCPP MOFs were successfully prepared by the coordination between iron ions and porphyrin ligands (TCPP). In addition, the peroxidase (POD) activity of Fe-TCPP was proved, in detail, Fe-TCPP could catalyze H 2 O 2 to produce ·OH. Herein, glucose oxidase (GOx) was chosen as the model to build cascade reaction by combining Fe-TCPP to detect glucose. The results indicated glucose could be detected by this cascade system selectively and sensitively, and the limit of detection of glucose was achieved to 0.12 μM. Furthermore, a portable hydrogel (Fe-TCPP@GEL) was further established, which encapsulated Fe-TCPP MOFs, GOx and TMB in one system. This functional hydrogel could be applied for colorimetric detection of glucose by coupling with a smartphone easily. Nanozyme of Fe-TCPP and corresponding hydrogel were constructed, and they were further developed to detect glucose. [Display omitted] • A simple and efficient nanozyme for the detection of glucose is reported. • A universal hydrogel system containing both natural enzymes and nanozymes is developed. • Fe-TCPP@GEL could carry out the quantitative analysis of glucose by coupling with a smartphone easily. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • The Erdaowa sequence was deposited at roughly the same time as the protoliths of the nearby khondalites. • O and Hf isotopic compositions of zircons suggest a high maturity of the metasedimentary rocks. • Rocks of same or similar age but with different metamorphic grades occur in the Khondalite Belt. • Tectonic slices were brought up to the same crustal level during the late Paleoproterozoic event. Abstract The Khondalite Belt is located in the northwestern North China Craton and contains late Paleoproterozoic metasedimentary rocks that generally underwent high-grade metamorphism. Only a few supracrustal sequences experienced low-grade metamorphism such as the Meidaizhao sequence in the Daqingshan area. The Erdaowa sequence north of Hohhot City, Inner Mongolia, is another late Paleoproterozoic supracrustal unit showing relatively low-grade metamorphism compared with the adjacent metapelitic khondalites. It is composed of metavolcanic as well as clastic metasedimentary rocks and marble. It was traditionally subdivided into the Halaqin and Hongshangou Formations. We undertook an integrated study of U-Pb zircon dating and O-Hf isotopic analysis, combined with whole-rock chemistry, on metasedimentary rocks of the Erdaowa sequence in order to understand the relationship between the low- and high-grade rocks and their tectono-thermal history in the Khondalite Belt. Cores and metamorphic domains of detrital zircons from a two-mica schist of the Halaqin Formation have 207Pb/206Pb ages of 2.0 and 1.86 Ga, δ18O values of 6.69–9.45‰ and 6.68–12.15‰, and ɛ Hf (t) values of +7.7 to +16.2 and +3.3 to +20.9, respectively. A recrystallized core, fir-tree domain and altered domain of zircon from a Grt-St-Ky two-mica gneiss have 207Pb/206Pb ages of >2.0 Ga, 1.88 Ga and 1.86 Ga, δ18O values of 12.10–12.75‰, 11.57–12.65‰ and 11.57–12.27‰, and ɛ Hf (t) values of +0.7 to +5.4, −2.0 to +4.7 and −2.0 to +0.5, respectively. These data limit the time of deposition of the sedimentary rocks between 2.0 and 1.88 Ga and show a high maturity, similar to khondalites of the same sequence. Compared with previous studies, we infer that the Erdaowa sequence consists of rocks of different origins and metamorphic grades (including khondalites), which were brought up to the same crustal level as tectonic slices due to late Paleoproterozoic tectonic processes. [ABSTRACT FROM AUTHOR]

Purpose To investigate the expression, clinical significance, biological function, and the potential mechanism of PHGDH in pancreatic cancer. Methods The expression of PHGDH in human pancreatic cancer tissues and corresponding adjacent normal tissues were analyzed through immunohistochemistry staining. Simultaneously, the association between the PHGDH expression and the clinicopathological parameters and OS and DFS was evaluated. Human pancreatic cancer cell line BxPC-3 and SW1990 were selected to investigate the effect of PHGDH knockdown on cell proliferation, migration, and invasion. In addition, we performed western blot to assess the expression of cyclin B1, and cyclin D1, MMP-2, and MMP-9 protein. Results Our results suggested that the expression of PHGDH is increased in pancreatic cancer compared with adjacent normal tissues and the increased expression of PHGDH is associated with tumor size, lymph node metastasis, and TNM state of pancreatic cancer patients. Moreover, the expression of PHGDH is an independent prognostic indicator for pancreatic cancer patients. In addition, we found that knockdown of PHGDH in pancreatic cancer cells inhibits the cell proliferation, migration, and invasion abilities by down-regulating the expression of cyclin B1, and cyclin D1, MMP-2, and MMP-9. Conclusions Our data indicated that the expression of PHGDH is increased in pancreatic cancer and is an independent molecular prognostic factor for pancreatic cancer patients. In addition, PHGDH controls cell proliferation, migration and invasion abilities. Therefore, PHGDH could serve as an important prognostic indicator and therapeutic target for pancreatic cancer. [ABSTRACT FROM AUTHOR]

It is well established now that neuronal dysfunction rather than structural damage may be responsible for the development of rabies. In order to explore the underlying mechanisms in rabies virus (RABV) and synaptic dysfunctions, a quantitative proteome profiling was carried out on synaptosome samples from mice hippocampus. Synaptosome samples from mice hippocampus were isolated and confirmed by Western blot and transmission electron microscopy. Synaptosome protein content changes were quantitatively detected by Nano-LC-MS/MS. Protein functions were classified by the Gene Ontology (GO) and KEGG pathway. PSICQUIC was used to create a network. MCODE algorithm was applied to obtain subnetworks. Of these protein changes, 45 were upregulated and 14 were downregulated following RABV infection relative to non-infected (mock) synaptosomes. 28 proteins were unique to mock treatment and 12 were unique to RABV treatment. Proteins related to metabolism and synaptic vesicle showed the most changes in expression levels. Furthermore, protein-protein interaction (PPI) networks revealed that several key biological processes related to synaptic functions potentially were modulated by RABV, including energy metabolism, cytoskeleton organization, and synaptic transmission. These data will be useful for better understanding of neuronal dysfunction of rabies and provide the foundation for future research. [ABSTRACT FROM AUTHOR]

Voluminous Neoarchean plutonic and supracrustal rocks are well developed in the Western Shandong Province of the North China Craton, which has been divided into a late Neoarchean crustally-derived granite belt, a middle to early Neoarchean ancient rock belt and a late Neoarchean juvenile rock belt. Earlier studies provided reconnaissance evidence for ∼2.60 Ga metamorphism in some areas. This study presents SHRIMP zircon U–Pb dating and O isotope analysis on nine metamorphosed igneous rock samples (gneissic tonalite, gneissic trondhjemite, gneissic quartz diorite and meta-gabbro) from the ancient rock belt. Magmatic zircons vary in age from 2.74 Ga to 2.60 Ga, whereas metamorphic and anatectic zircons vary in age from 2.63 Ga to 2.59 Ga, with an age peak of 2.61 Ga. In this study, we identified 2.69–2.67 Ga metamorphic zircons for the first time. Most of zircons, whether magmatic or metamorphic, have δ 18 O values of +4.5‰ to +6.5‰ (V-SMOW). However, the oldest tonalite sample with a protolith age of 2735 Ma has zircons showing low δ 18 O (V-SMOW) values, particularly in recrystallized domains and overgrowths. This suggests at least in local crustal extension and influx of meteoric water during the evolution of the rocks. Combined with data from earlier studies, we draw the conclusion that the Western Shandong Province experienced a regional tectono-thermal event at ∼2.60 Ga, as a result of high heat flow and crustal extension, perhaps caused by a mantle plume or mantle overturn activity. Therefore we conclude that 2.60 Ga can be regarded the break between early and late Neoarchean crustal accretion. The O isotope study indicates that in some cases, trondhjemitic leucosome was not derived from nearby gneissic tonalite, although they show a close relationship in outcrop. [ABSTRACT FROM AUTHOR]

Background: Several case-control studies investigating the relationship between genetic polymorphisms of glutathione S-transferase (GST) M1, GSTT1, and GSTP1 (rs1695) and the risk of breast cancer have reported contradictory results. We therefore performed a meta-analysis to clarify this issue.Materials and Methods: An updated meta-analysis using PubMed and Web of Knowledge databases for the eligible case-control studies was performed. Random- or fixed-effects model was used.Results: A total of 10,067 cancer cases and 12,276 controls in 41 independent case-control studies from 19 articles were included in this meta-analysis. Significant increase in risk of breast cancer for Asians was found in GSTM1-null genotype (P=0.012, odds ratio [OR] =1.17, 95% confidence interval [CI] =1.04-1.32) and GSTT1-null genotype (P=0.039, OR =1.19, 95% CI =1.01-1.41). In addition, our results showed that the GSTP1 (rs1695) polymorphisms can significantly increase the risk among Caucasians (P=0.042, OR =1.16, 95% CI =1.01-1.34). Sensitivity analysis and publication bias further confirmed the dependability of the results in this meta-analysis.Conclusion: Our results demonstrate that both GSTM1- and GSTT1-null polymorphisms are associated with an increased risk of breast cancer in Asians and that GSTP1 Val105Ile (rs1695) polymorphism is associated with an increased breast cancer risk in Caucasians. [ABSTRACT FROM AUTHOR]

The new method presented in this article achieved the goal of capturing Salmonella typhimurium via immunoreaction and rapid in situ detection of the CdSe/ZnS quantum dots (QDs) labeled S. typhimurium by self-assembly light-emitting diode-induced fluorescence detection (LIF) microsystem on a specially designed multichannel microfluidic chip. CdSe/ZnS QDs were used as fluorescent markers improving detection sensitivity. The microfluidic chip developed in this study was composed of 12 sample channels, 3 mixing zones, and 6 immune reaction zones, which also acted as fluorescence detection zones. QDs–IgG–primary antibody complexes were generated by mixing CdSe/ZnS QDs conjugated secondary antibody (QDs–IgG) and S. typhimurium antibody (primary antibody) in mixing zones. Then, the complexes went into immune reaction zones to label previously captured S. typhimurium in the sandwich mode. The capture rate of S. typhimurium in each detection zone was up to 70%. The enriched QDs-labeled S. typhimurium was detected using a self-assembly LIF microsystem. A good linear relationship was obtained in the range from 3.7 × 10 to 3.7 × 10 5 cfu mL −1 using the equation I = 0.1739 log ( C ) − 0.1889 with R 2 = 0.9907, and the detection limit was down to 37 cfu mL −1 . The proposed method of online immunolabeling with QDs for in situ fluorescence detection on the designed multichannel microfluidic chip had been successfully used to detect S. typhimurium in pork sample, and it has shown potential advantages in practice. [ABSTRACT FROM AUTHOR]

Eastern Shandong is one of the important early Precambrian areas in the North China Craton, where extensive 2.5, 2.7 and 2.9 Ga magmatic activities have been identified, followed by very strong late Neoarchean and Paleoproterozoic tectono-thermal events. We carried out SHRIMP zircon dating, Hf-in-zircon isotope analysis and a whole-rock geochemical study on ca. 2.9 Ga Mesoarchean magmatic rocks. These include gneissic diorite, gneissic quartz diorite, gneissic tonalite and gneissic high-Si trondhjemite and were identified in five new areas. All these rocks contain ca. 2.5 Ga metamorphic zircons. The dioritic and tonalitic rocks are enriched in large ion lithosphile (LIL) elements (K, Rb, Ba) and depleted in Nb and P with moderate to highly fractionated REE patterns, similar in chemical compositions to Archean TTG rocks worldwide. The high-Si trondhjemites are characterized by very high SiO 2 and very low FeOt + MgO. The magmatic zircon grains from all these rocks are similar in their Hf isotopic compositions and have ɛ Hf ( t ) values ranging from + 1.3 to +9.9. Combined with previous work, our main conclusions are: (1) eastern Shandong underwent a major magmatic event at ca. 2.9 Ga; (2) diorites and tonalites formed by melting of thickened lower crust or the root of an oceanic plateau, whereas the high-Si trondhjemites were derived from melting of intermediate to felsic rocks; (3) the Mesoarchean (ca. 2.9 Ga) was a major period of juvenile crustal addition from the depleted mantle, earlier than the major crustal growth period at 2.7–2.8 Ga in the North China Craton; (4) the 2.7–2.9 Ga rocks underwent strong metamorphism at ca. 2.5 Ga, probably as a result of magmatic underplating. [ABSTRACT FROM AUTHOR]

Traumatic multidrug-resistant (MDR) bacterial infections are deadly threat to the public. To combat MDR bacteria, we developed a dual functional pH-sensitive hydrogel based on peptide DP7 (VQWRIRVAVIRK) and oxidized dextran (DP7-ODEX hydrogel). As an antimicrobial peptide, DP7 can synergize with many antibiotics; thus, we loaded ceftazidime into DP7-ODEX hydrogel, which showed an obvious advantage in MDR P. aeruginosa inhibition. Additionally, due to the interaction between aldehyde groups in oxidized dextran and amine groups from wound tissue, the hydrogel could extend on the irregular surface of skin defects and promote epithelial cells adhesion. DP7 could also be used as a wound-healing peptide and accelerate the healing process. We confirmed that the DP7-ODEX hydrogel exerted formidable therapeutic effects in normal or diabetic wound infection model. According to histomorphology analysis we found that DP7 hydrogel also have a scarless wound healing ability. In summary, we developed a hydrogel fabricated by the dual functional peptide DP7 that can kill multidrug-resistant bacteria colonizing the wound bed and boost scarless wound healing. • Antibacterial peptide is used to fabricate hydrogel network and promote scarless wound healing process. • A synergistic strategy was used to treat multidrug resistant bacteria. • The hydrogel can inhibit multidrug resistant bacterial growth without inducing its resistance. • The hydrogel can inhibit multidrug resistant bacterial and biofilm growth by using a low dosage of antibiotics. [ABSTRACT FROM AUTHOR]