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Your search keyword '"Durga Prasad Cherukuri"' showing total 4 results
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4 results on '"Durga Prasad Cherukuri"'

1. Instability of a dinucleotide repeat in the 3′‐untranslated region (UTR) of the microsomal prostaglandin E synthase‐1 (mPGES‐1) gene in microsatellite instability‐high (MSI‐H) colorectal carcinoma

Author

Durga Prasad Cherukuri, Joshua L. Deignan, Kingshuk Das, Wayne W. Grody, and Harvey Herschman

Subjects
mPGES-1, Dinucleotide repeat, Microsatellite instability, Colorectal cancer, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

DNA mismatch‐repair gene mutations, with consequent loss of functional protein expression, result in microsatellite instability (MSI). Microsatellite sequences are found in coding regions and in regulatory regions of genes (i.e., 5′‐UTRs and 3′‐UTRs). In addition to being a surrogate marker of defective mismatch repair, deletion or insertion microsatellite sequences can dysregulate gene expression in MSI‐H (microsatellite instability‐high) tumors. The microsomal prostaglandin E synthase‐1 (mPGES‐1) gene product, mPGES‐1, participates in prostaglandin E2 (PGE2) production. Moreover, mPGES‐1 is often overexpressed in human colorectal tumors, and is thought to contribute to progression of these tumors. Here we identified a dinucleotide repeat, (GT)24, in the mPGES‐1 gene 3′ untranslated region (3′‐UTR), and analyzed its mutation frequencies in MSI‐H and microsatellite stable (MSS) tumors. The (GT)24 repeat exhibited instability in all MSI‐H tumors examined (14), but not in any of the MSS tumors (13). In most cases, (GT)24 repeat instability resulted in insertion of additional GT units. We also determined mPGES‐1 mRNA levels in MSI‐H and MSS colorectal cancer cell lines. Three of four previously designated “MSI‐H” cell lines showed higher mPGES‐1 mRNA levels compared to MSS cell lines; correlations between elevated mPGES‐1 mRNA levels and microsatellite (GT)24 repeat characteristics are present for all six cell lines. Our results demonstrate that mPGES‐1 is a target gene of defective mismatch repair in human colorectal cancer, with functional consequence.

Published
2015
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2. RNA sequencing for the detection of MET exon 14 skipping in specimens with splice-site mutations

Author

Paris Petersen, Lauryn Keeler, Gregory Sims, Francesca Fike, Dana Weiner, Jeffrey Falk, Durga Prasad Cherukuri, Yu Xia, Armin Graber, Maya Panjikaran, Christopher J. Scott, Parth Sitlani, and Richard Tseng

Subjects
Cancer Research, Exon, Oncology, business.industry, RNA splicing, RNA, Medicine, business, Molecular biology, DNA sequencing
Abstract

e24178Background: MET exon 14 skipping is detected in 2%-4% of NSCLC and is known to confer increased sensitivity to MET inhibitors. Over 100 distinct DNA sequence variants have been identified tha...

Published
2018
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3. Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat

Author

S. Koul, Ram Badan Singh, Qazi Mohammad Rizwanul Haq, Durga Prasad Cherukuri, A. Charpe, S. K. Gupta, and Kumble Vinod Prabhu

Subjects
Genetics, biology, food and beverages, Virulence, Locus (genetics), Plant Science, Horticulture, Marker-assisted selection, Amplicon, biology.organism_classification, RAPD, Aegilops speltoides, chemistry.chemical_compound, chemistry, Agronomy and Crop Science, Gene, DNA
Abstract

In a segregating homozygous F2 population of bread wheat involving a leaf rust resistance gene Lr28 derived from Aegilops speltoides, six randomly amplified polymorphic DNA (RAPD) markers, three each in coupling and repulsion phase were identified as linked to Lr28, mapped to a region spanning 32 cM including the locus. The F2 and F3 populations were studied in the phytotron challenged with the most virulent pathotype 77-5 of leaf rust. A coupling phase linked RAPD marker S464721 and a repulsion phase linked RAPD marker S326550 flanked the gene Lr28 by a distance of 2.4± 0.016 cM on either side. The flanking markers genetically worked as co-dominant markers when analyzed together after separate amplification in the F2 population by distinguishing the homozygotes from the heterozygotes and increased the efficiency of marker assisted selection by reducing the false positives and negatives. One of the three RAPD markers, S421640 was converted to locus specific SCAR marker SCS421640 which was further truncated by designing primers internal from both ends of the original RAPD amplicon to eliminate a non-specific amplification of nearly same size. The truncated polymorphic sequence characterized amplified region marker (TPSCAR) SCS421570 was 70 bp smaller, but resulted in a single band polymorphism specific to Lr28 resistance. The TPSCAR marker was validated for its specificity to the gene Lr28 in nine different genetic backgrounds and on 43 of the 50 Lr genes of both native and alien origin, suggesting the utility of the SCAR markers in pyramiding leaf rust resistance genes in wheat.

Published
2005
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