13 results on '"Durval, Italo José Batista"'
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2. ANÁLISE DAS CARACTERÍSTICAS FÍSICO-QUÍMICAS DA CELULOSE BACTERIANA PRODUZIDA EM MEIO ALTERNATIVO À BASE DE TOMATE (SOLANUM LYCOPERSICUM)
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Cavalcanti, Yasmim de Farias, primary, Medeiros, Alexandre D’Lamare Maia de, additional, Silva Junior, Cláudio José Galdino da, additional, Durval, Italo José Batista, additional, Amorim, Julia Didier Pedrosa de, additional, Costa, Andréa Fernanda de Santana, additional, and Sarubbo, Leonie Asfora, additional
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- 2023
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3. Treatment of Oily Effluents Using a Bacterial Cellulose Membrane as the Filter Bed
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Medeiros, Alexandre D’Lamare Maia de, primary, Silva Junior, Cláudio José Galdino da, additional, Durval, Italo José Batista, additional, Souza, Thais Cavalcante de, additional, Cavalcanti, Yasmim de Farias, additional, Costa, Andréa Fernanda de Santana, additional, and Sarubbo, Leonie Asfora, additional
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- 2024
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4. Application of Microbial Biosurfactants in the Food Industry
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Durval, Italo José Batista, da Silva, Ivison Amaro, Sarubbo, Leonie Asfora, Prasad, Ram, Series Editor, Inamuddin, editor, and Ahamed, Mohd Imran, editor
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- 2021
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5. Potential Applications of Anti-Adhesive Biosurfactants
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da Silva, Maria da Gloria Conceição, Durval, Italo José Batista, da Silva, Maria Eduarda Pereira, Sarubbo, Leonie Asfora, Prasad, Ram, Series Editor, Inamuddin, editor, and Ahamed, Mohd Imran, editor
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- 2021
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6. Towards Sustainable Packaging Using Microbial Cellulose and Sugarcane (Saccharum officinarum L.) Bagasse.
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da Silva Junior, Cláudio José Galdino, de Medeiros, Alexandre D'Lamare Maia, Cavalcanti, Anantcha Karla Lafaiete de Holanda, de Amorim, Julia Didier Pedrosa, Durval, Italo José Batista, Cavalcanti, Yasmim de Farias, Converti, Attilio, Costa, Andréa Fernanda de Santana, and Sarubbo, Leonie Asfora
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RAW materials ,SUSTAINABLE design ,SUGARCANE ,PACKAGING recycling ,WASTE minimization ,BAGASSE - Abstract
The high consumption of packaging has led to a massive production of waste, especially in the form of nonbiodegradable polymers that are difficult to recycle. Microbial cellulose is considered a biodegradable, low-cost, useful, ecologically correct polymer that may be joined with other biomaterials to obtain novel characteristics and can, therefore, be used as a raw material to produce packaging. Bagasse, a waste rich in plant cellulose, can be reprocessed and used to produce and reinforce other materials. Based on these concepts, the aim of the current research was to design sustainable packaging material composed of bacterial cellulose (BC) and sugarcane bagasse (SCB), employing an innovative shredding and reconstitution method able to avoid biomass waste. This method enabled creating a uniform structure with a 0.10-cm constant thickness, classified as having high grammage. The developed materials, particularly the 0.7 BC/0.3 SCB [70% (w/w) BC plus 30% (w/w) SCB] composite, had considerable tensile strength (up to 46.22 MPa), which was nearly thrice that of SCB alone (17.43 MPa). Additionally, the sorption index of the 0.7 BC/0.3 SCB composite (235.85 ± 31.29 s) was approximately 300-times higher than that of SCB (0.78 ± 0.09 s). The packaging material was also submitted to other analytical tests to determine its physical and chemical characteristics, which indicated that it has excellent flexibility and can be folded 100 times without tearing. Its surface was explored via scanning electron microscopy, which revealed the presence of fibers measuring 83.18 nm in diameter (BC). Greater adherence after the reconstitution process and even a uniform distribution of SCB fibers in the BC matrix were observed, resulting in greater tear resistance than SCB in its pure form. The results demonstrated that the composite formed by BC and SCB is promising as a raw material for sustainable packaging, due to its resistance and uniformity. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Treatment of Oily Effluents Using a Bacterial Cellulose Membrane as the Filter Bed.
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Medeiros, Alexandre D'Lamare Maia de, Silva Junior, Cláudio José Galdino da, Durval, Italo José Batista, Souza, Thais Cavalcante de, Cavalcanti, Yasmim de Farias, Costa, Andréa Fernanda de Santana, and Sarubbo, Leonie Asfora
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INDUSTRIAL wastes ,WATER purification ,WASTEWATER treatment ,WATER analysis ,MEMBRANE separation - Abstract
One of the main challenges in the treatment of industrial wastewater is the removal of oil-in-water emulsions, which are stable and therefore difficult to treat. Bacterial cellulose (BC) has structural characteristics that make it an ideal filtration membrane. Several research projects are underway to develop new materials, both biotechnological and traditional, for use in filter beds. The study examined the potential of a BC membrane filtration system for treating oily industrial wastewaters, an underexplored biomaterial in wastewater treatment. The results demonstrated that BC is highly effective at removing oily contaminants (~99%), reducing the colour and particulate matter of wastewater, as well as eliminating nearly the entire microbiological load (~99%). SEM, MEV, FTIR, XRD, and TGA confirmed the presence of oil in the interior of the membrane after filtration, characteristic peaks of its chemical composition, and a 40% reduction in crystallinity. TGA revealed an increase from three (pre-filtration) to five (post-filtration) stages of thermal degradation, indicating the retention of the contaminant in the BC. The mechanical tests demonstrated that the membrane has a tensile strength of 72.13 ± 8.22 MPa and tolerated elongation of up to 21.11 ± 4.81% prior to tearing. The BC membrane also exhibited excellent flexibility, as it could be folded >100 times at the same point without exhibiting signs of tearing. The BC surpasses traditional methods, such as activated charcoal and effluent treatment stations, in the removal of emulsified oils. The findings demonstrate that BC is promising for the treatment of industrial wastewaters, which is a field that requires continual technological innovations to mitigate the environmental impacts of the oil industry. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Synthesis of Transparent Bacterial Cellulose Films as a Platform for Targeted Drug Delivery in Wound Care.
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Amorim, Julia Didier Pedrosa de, Cavalcanti, Yasmim de Farias, Medeiros, Alexandre D'Lamare Maia de, Silva Junior, Cláudio José Galdino da, Durval, Italo José Batista, Costa, Andréa Fernanda de Santana, and Sarubbo, Leonie Asfora
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TARGETED drug delivery ,DRUG delivery systems ,CONTROLLED release drugs ,WOUND care ,LOCAL anesthetics - Abstract
Bacterial cellulose (BC) can be chemically modified and combined with other materials to create composites with enhanced properties. In the medical field, biomaterials offer advantages, such as biocompatibility and sustainability, enabling improved therapeutic strategies and patient outcomes. Incorporating lidocaine into wound dressings offers significant potential benefits. In this study, transparent BC films were produced in situ with an undefined minimal culture medium with a yeast and bacteria co-culture system on black tea (Camellia sinensis) and white sugar medium for three days. Lidocaine was incorporated ex situ into the BC matrix, and the composite film was sterilized using gamma radiation. Drug-release studies showed a two-stage release profile, with an initial fast release (24.6%) followed by a slower secondary release (27.2% cumulative release). The results confirmed the incorporation of lidocaine into the BC, producing highly transparent films with excellent thermal stability, essential for the storage and transportation of wound dressings. This study highlighted BC properties and drug incorporation and release behavior. The findings contribute towards optimizing wound dressings with controlled drug release, showcasing the potential of transparent BC films as an effective platform for wound care and drug-delivery applications. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Design and Modeling of a Biotechnological Nanofiltration Module Using Bacterial Cellulose Membranes for the Separation of Oily Mixtures
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Medeiros, Alexandre D’Lamare Maia de, primary, Silva Junior, Cláudio José Galdino da, additional, Amorim, Júlia Didier Pedrosa de, additional, Durval, Italo José Batista, additional, Damian, Ricardo Barbosa, additional, Cavalcanti, Yasmim de Farias, additional, Costa, Andréa Fernanda de Santana, additional, and Sarubbo, Leonie Asfora, additional
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- 2023
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10. Oily Wastewater Treatment: Methods, Challenges, and Trends
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Medeiros, Alexandre D’Lamare Maia de, primary, Silva Junior, Cláudio José Galdino da, additional, Amorim, Julia Didier Pedrosa de, additional, Durval, Italo José Batista, additional, Costa, Andréa Fernanda de Santana, additional, and Sarubbo, Leonie Asfora, additional
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- 2022
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11. Green Synthesis of Silver Nanoparticles Using a Biosurfactant from Bacillus cereus UCP 1615 as Stabilizing Agent and Its Application as an Antifungal Agent
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Durval, Italo José Batista, primary, Meira, Hugo Morais, additional, de Veras, Bruno Oliveira, additional, Rufino, Raquel Diniz, additional, Converti, Attilio, additional, and Sarubbo, Leonie Asfora, additional
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- 2021
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12. Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais
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DURVAL, Italo José Batista, SARUBBO, Leonie Asfora, RUFINO, Raquel Diniz, SANTOS, Valdemir Alexandre dos, LUNA, Juliana Moura de, SILVA, Rita de Cássia Freire Soares da, and GUERRA, Jenyffer Medeiros Campos
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Bacillus cereus ,Biorremediação ,CIENCIAS [OUTROS] ,Biossurfactante ,Poluição ,Toxicidade ,Biotecnologia - Abstract
Submitted by (lucia.rodrigues@ufrpe.br) on 2022-12-21T19:31:54Z No. of bitstreams: 1 Italo Jose Batista Durval.pdf: 1815528 bytes, checksum: cab851fb72bcd916e5a321992400269b (MD5) Made available in DSpace on 2022-12-21T19:31:54Z (GMT). No. of bitstreams: 1 Italo Jose Batista Durval.pdf: 1815528 bytes, checksum: cab851fb72bcd916e5a321992400269b (MD5) Previous issue date: 2021-07-30 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq The present study characterized and evaluated the biosurfactant produced by Bacillus cereus UCP 1615 in various industrial applications. The production of the biomolecule was carried out in a low-cost medium composed of 2% residual frying soybean oil and 0.12% peptone. The interfacial tension reducing capacity and the critical micelle concentration (CMC) were defined as 6 mN/m and 500 mg/L, respectively. Large-scale production was carried out in 1.2, 3.0 and 50 L bioreactors, reaching surface tension values of 28.7, 27.5 and 32 mN/m and yields around 4.3, 4.6 and 4.1 g/L, respectively. The biosurfactant was classified as an anionic lipopeptide from analyzes to determine ionic charge, thin layer chromatography (TLC), gas chromatography (GC) and nuclear magnetic resonance (NMR). Long-term conservation tests with the additive potassium sorbate (0.2%) demonstrated the stability of the surface-active properties of the biosurfactant, suggesting its application in a possible commercial formulation. To evaluate the application of the biosurfactant in environmental processes, it was applied in tests containing seawater, soil and rocks contaminated with petroleum derivatives and its toxicity was also evaluated against marine organisms Artemia salina (microcrustacean), Poecilia vivipara (fish) and Anomalocardia brasiliana (bivalve) and to the seeds of vegetables Brassica oleracea (cabbage), Solanum lycopersicum (tomato) and Cucumis anguria (gherkin). The biosurfactant proved to be efficient and biocompatible for bioremediation tests in seawater, promoting the growth of the microbiota, and showed removal rates of hydrophobic compounds in sandy soil and rocks of 84 and 69%, respectively. The application of the biomolecule as a bioemulsifier in food was also evaluated through its use in the composition of a cookie type cookie, with cytotoxicity using the L929 and Vero cell lines and the antioxidant activity determined. A biscuit with preserved typical characteristics and benefits compared to the standard without biosurfactant was obtained. Cytotoxicity tests reinforced the biosurfactant's biocompatibility, and the antioxidant activity indicative corroborated with the biosurfactant's contribution to the biscuit preservation. The biosurfactant was also tested as a stabilizer in the synthesis of silver nanoparticles as an antimicrobial agent. Nanoparticles of size around 20 nm were obtained using the green Tollens method. The nanoparticles showed fungicidal activity against human and plant pathogenic fungal genera. The results obtained demonstrate that the biosurfactant from Bacillus cereus UCP 1615 has potential for use in several economic sectors, proving the biotechnological versatility of this biomolecule and its industrial importance. O presente estudo caracterizou e avaliou o biossurfactante produzido por Bacillus cereus UCP 1615 em diversas aplicações industriais. A produção da biomolécula foi feita em meio de baixo custo composto por 2% de óleo de soja residual de fritura e 0,12% de peptona. A capacidade de redução da tensão interfacial e a concentração micelar crítica (CMC) foram definidas como 6 mN/m e 500 mg/L, respectivamente. A produção em larga escala foi feita em biorreatores de 1,2, 3,0 e 50,0 L, alcançando valores de tensão superficial de 28,7, 27,5 e 32,0 mN/m e rendimentos em torno de 4,3, 4,6 e 4,1 g/L, respectivamente. O biossurfactante foi classificado como um lipopetídeo aniônico a partir de análises para determinação da carga iônica, cromatografia em camada delgada (TLC), cromatografia gasosa (GC) e ressonância magnética nuclear (RMN). Testes de conservação a longo prazo com o aditivo sorbato de potássio (0,2%) demonstraram a estabilidade das propriedades tensoativas do biossurfactante, sugerindo sua aplicação em uma possível formulação comercial. Para avaliar a aplicação do biossurfactante em processos ambientais, o mesmo foi aplicado em ensaios contendo água do mar, solo e rochas contaminadas com derivado de petróleo e sua toxicidade também foi avaliada frente ao microcrustáceo Artemia salina, ao peixe Poecilia vivipara e ao bivalve Anomalocardia brasiliana, bem como frente às sementes de vegetais Brassica oleracea (repolho), Solanum lycopersicum (tomate) e Cucumis anguria (maxixe). O biossurfactante mostrou-se eficiente e biocompatível para biorremediação em água do mar, promovendo o crescimento da microbiota, e ainda apresentou índices de remoção de compostos hidrofóbicos em solo arenoso e rochas de 84 e 69%, respectivamente. A aplicação da biomolécula como bioemulsificante em alimentos também foi avaliada através da sua utilização na composição de um biscoito tipo cookie, tendo a citotoxicidade utilizando as linhagens celulares L929 e Vero e a atividade antioxidante determinados. Um biscoito com características típicas preservadas e com benefícios em relação ao padrão sem biossurfactante foi obtido. Os testes de citotoxicidade reforçaram a biocompatibilidade do biossurfactante, e o indicativo de atividade antioxidante corroborou com a contribuição do biossurfactante para preservação do biscoito. O biossurfactante também foi avaliado como estabilizante na síntese de nanopartículas de prata como agente antimicrobiano. Nanopartículas de tamanho em torno de 20 nm foram obtidas através do método verde de Tollens. As nanopartículas apresentaram atividade fungicida diante de gêneros de fungos patogênicos humanos e vegetais. Os resultados obtidos demonstram que o biossurfactante de Bacillus cereus UCP 1615 tem potencial de utilização em diversos setores econômicos, comprovando a versatilidade biotecnológica desta biomolécula e sua importância industrial.
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- 2021
13. Estudo de um biossurfactante produzido por espécies de Bacillus isoladas de água do mar e seu potencial para biorremediação de derramamento de petróleo
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DURVAL, Italo José Batista, SARUBBO, Leonie Asfora, and RUFINO, Raquel Diniz
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Biodegradação ,Biossurfactantes ,Bacillus (bactéria) - Abstract
CAPES Derramamentos de petróleo representam um perigo permanente para o desenvolvimento mundial. Métodos de limpeza e remediação são fundamentai para evitar a intoxicação e a morte de organismos vivos após desastres com hidrocarbonetos. Os biossurfactantes são anfipáticos e reduzem as tensões superficiais e interfaciais para melhorar a mistura de duas fases imiscíveis. Esses compostos naturais têm menos toxicidade, mais biodegradabilidade e estabilidade sob condições ambientais adversas em comparação com surfactantes químicos. Nesse sentido, o trabalho teve como objetivo isolar uma bactéria produtora de biossurfactante e caracterizar-lo para aplicação na bioremediação de ambientes marinhos. As bactérias produtoras de biossurfactantes foram isoladas de amostras de água do mar e identificadas como pertencentes ao gênero Bacillus. As bactérias foram cultivadas com diferentes fontes de carbono (glicose, óleo de soja e óleo de fritura de soja) e nitrogênio (cloreto de amônio, nitrato de sódio, ureia e peptona). O isolado Bacillus sp. apresentou os resultados mais promissores para produção de biossurfactante, sendo cultivado em meio mineral suplementado com 2% de óleo de fritura e 0,12% de peptona. Após a avaliação da agitação e do tempo de cultivo, foram selecionadas as condições: 250 rpm e 48 horas. Nestas condições, a tensão superficial foi reduzida para 27 mN/m, a interfacial para 6 mN/m e a produção de biossurfactante foi de 3,5 g/L. O aumento de escala foi realizado em biorreatores, tendo como resultados: tensões superficiais de 28,7; 27,5 e 32 mN/m e rendimentos de 4,3; 4,6 e 4,1 g/L para biorreatores de 1,2 ; 3 e 50 L, respectivamente. O biossurfactante foi caracterizado como um lipopeptídeo aniônico com CMC de 500 mg/L. O tensoativo aumentou a degradação do óleo motor para até 96% em 27 dias de incubação em água do mar em relação ao controle e apresentou capacidade de dispersão de óleo motor (superior a 80%). Assim, o biossurfactante apresenta potencial na remediação de ambientes marinhos contaminados com petroderivados. Oil spills represent a permanent danger to global development. Methods of cleaning and remediation are critical to avoid intoxication and death of organisms after disasters with hydrocarbons. The biosurfactants are amphipathic and reduce surface and interfacial tensions to improve a mixture of two immiscible phases. These compounds are less toxic, more biodegradable and stable under adverse environmental conditions compared to chemical surfactants. In this sense, the objective of this work was to isolate a biosurfactant-producing bacterium and characterize it for application in the biography of marine environments Biosurfactant-producing bacteria were isolated from seawater samples and identified as belonging to the genus Bacillus. The bacteria were cultivated with different carbon (glucose, soybean oil and waste frying soybean oil) and nitrogen (ammonium chloride, sodium nitrate, urea and peptone) sources. The isolate Bacillus sp. presented the most promising results for biosurfactant production, being cultivated in mineral medium supplemented with 2% frying oil and 0.12% peptone. After the evaluation of the agitation and the culture time, the conditions were selected: 250 rpm and 48 hours. Under these conditions, the surface tension was reduced to 27 mN/m, the interfacial to 6 mN/m and the biosurfactant yield was 3.5 g/L. Scale-up production was performed in bioreactors, having as results: surface tensions of 28.7, 27.5 and 32 mN/m and yields of 4.3, 4.6 and 4.1 g/L for bioreactors of 1.2; 3 and 50-L, respectively. The biosurfactant was characterized as an anionic lipopeptide with CMC of 500 mg/L. The surfactant increased the degradation of the motor oil to up to 96% in 27 days of incubation in sea water in relation to the control and showed a dispersion capacity of motor oil (above 80%).Thus, the biosurfactant presents potential in the remediation of marine environments contaminated with petroderivatives.
- Published
- 2017
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