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Background and aims: Intercrops offer multiple advantages over sole crops. The aim of our study was to characterize root growth and interactions in spring wheat/faba bean intercrops to better understand belowground interactions that govern resource capture. Materials and methods: A field experiment was conducted with one faba bean cultivar and two spring wheat cultivars sown at three sowing densities, defining three intercropping designs. Destructive root coring was conducted (0–100 cm) in the intercrops and sole crops at two development stages. FTIR spectroscopy was used to discriminate the species' root masses. The plant-plant interaction index was calculated to represent the belowground interactions. Results: A negative impact of intercropping on total root mass was observed in the treatment with high sowing density in both stages. For the fully and partial replacement design treatments, plant-plant facilitation was more pronounced than competition in all layers. Competition dominated root growth in the treatment with high sowing density in both stages. Lower sowing densities encouraged deep root growth of wheat (both cultivars) in intercropping. The early root growth in depth and in density of one spring wheat cultivar impacted negatively faba bean root growth. Intercropping resulted in a grain yield advantage in both fully and only one partial replacement design treatment. Conclusion: In the intercrops, total root mass and plant-plant interactions were affected more by sowing density than by the spring wheat cultivar. Understanding the effect of sowing density on root growth in intercropping can help to support the design of sustainable intercropping systems.

One foundational assumption of trait-based ecology is that traits can predict species demography. However, the links between traits and demographic rates are, in general, not as strong as expected. These weak associations may be due to the use of traits that are distantly related to performance, and/or the lack of consideration of size-related variations in both traits and demographic rates. Here, we examined how wood traits were related to demographic rates in 19 tree species from a lowland forest in eastern Amazonia. We measured 11 wood traits (i.e. structural, anatomical and chemical traits) in sapling, juvenile and adult wood; and related them to growth and mortality rates (MR) at different ontogenetic stages. The links between wood traits and demographic rates changed during tree development. At the sapling stage, relative growth rates (RGR) were negatively related to wood specific gravity (WSG) and total parenchyma fractions, while MR decreased with radial parenchyma fractions, but increased with vessel lumen area (VA). Juvenile RGR were unrelated to wood traits, whereas juvenile MR were negatively related to WSG and axial parenchyma fractions. At the adult stage, RGR scaled with VA and wood potassium concentrations. Adult MR were not predicted by any trait. Overall, the strength of the trait-demography associations decreased at later ontogenetic stages. Our results indicate that the associations between traits and demographic rates can change as trees age. Also, wood chemical or anatomical traits may be better predictors of growth and MR than WSG. Our findings are important to expand our knowledge on tree life-history variations and community dynamics in tropical forests, by broadening our understanding on the links between wood traits and demography during tree development.

Background: Roots play an important role during plant growth and development, ensuring water and nutrient uptake. Understanding the mechanisms regulating their initiation and development opens doors towards root system architecture engineering. Results: Here, we investigated by RNA-seq analysis the changes in gene expression in the barley stem base of 1 day-after-germination (DAG) and 10DAG seedlings when crown roots are formed. We identified 2,333 genes whose expression was lower in the stem base of 10DAG seedlings compared to 1DAG seedlings. Those genes were mostly related to basal cellular activity such as cell cycle organization, protein biosynthesis, chromatin organization, cytoskeleton organization or nucleotide metabolism. In opposite, 2,932 genes showed up-regulation in the stem base of 10DAG seedlings compared to 1DAG seedlings, and their function was related to phytohormone action, solute transport, redox homeostasis, protein modification, secondary metabolism. Our results highlighted genes that are likely involved in the different steps of crown root formation from initiation to primordia differentiation and emergence, and revealed the activation of different hormonal pathways during this process. Conclusions: This whole transcriptomic study is the first study aiming at understanding the molecular mechanisms controlling crown root development in barley. The results shed light on crown root emergence that is likely associated with a strong cell wall modification, death of the cells covering the crown root primordium, and the production of defense molecules that might prevent pathogen infection at the site of root emergence.

L'effet de la pâture sur l'évolution de la masse herbacée et sa digestibilité au cours de l'année a été étudié à Dahra, dans le Sahel sénégalais. Dans cette zone d'élevage la strate herbacée, base de l'alimentation du bétail, a une croissance brève pendant la saison des pluies. L'étude a été conduite pendant la saison des pluies et la saison sèche sur deux années consécutives, de juillet 2018 à juin 2020. Les mesures de phytomasse et de qualité fourragère (digestibilité in vitro de la matière organique, DMO) ont démarré dix jours après la première pluie et ont été répétées avec une fréquence de dix jours en saison des pluies et trente en saison sèche. La dynamique des herbacées annuelles se fait en trois étapes : une première de faible croissance, une deuxième de croissance rapide et une troisième de sénescence. La quantité maximale de masse sur pied sous pâture était de 1 992 ± 755 kg MS.ha-1 en 2018 et de 1 801 ± 601 kg MS.ha-1 en 2019 alors que, dans la mise en défens, elle était de 2022 ± 375 kg MS.ha-1 en 2018 et de 2357 ± 664 kg MS.ha-1 en 2019. La DMO a fortement décru au cours de la saison de végétation : de 70 % en début de saison des pluies, elle était en moyenne de 52 % en pleine saison des pluies et 30 % en saison sèche. La matière organique du fourrage a été plus digestible sous pâture que sous mise en défens. La pâture n'a pas systématiquement diminué la masse sur pied disponible de façon significative, mais en revanche elle en a amélioré la digestibilité.

Tree growth is key to species performance. However, individual growth variability within species remains underexplored for a whole community, and the role of species evolutionary legacy and local environments remains unquantified. Based on 36 years of diameter records for 7961 trees from 138 species, we assessed individual growth across an Amazonian forest. We related individual growth to taxonomy, topography and neighbourhood, before exploring species growth link to functional traits and distribution along the phylogeny. We found most variation in growth among individuals within species, even though taxonomy explained a third of the variation. Species growth was phylogenetically conserved up to the genus. Traits of roots, wood and leaves were good predictors of growth, suggesting their joint selection during convergent evolutions. Neighbourhood crowding significantly decreased individual growth, although much of inter-individual variation remains unexplained. The high intraspecific variation observed could allow individuals to respond to the heterogeneous environments of Amazonian forests.

1. Identifying and quantifying factors that influence tree growth are crucial issues to ensure sustainable forest management, particularly in moist tropical forests. Tree growth depends on several factors comprising ontogenic stage, competition by neighbours and environmental conditions. Several studies have focused on one or two of them, but very few have considered all three, especially in Central Africa. We investigated the effects of diameter and competition on tree growth, in four Central African sites characterized by their soil physicochemical properties, at both tree community and population levels. 2. We calibrated growth models using diameter data collected on 29,741 trees between 2015 and 2018, on twelve 4 or 9-ha plots spread over the four sites. These models included diameter, wood density, competition indices and site effect as explainable variables at the community level and excluded wood density at the population level. 3. At the community level, the best models explained 11% of growth variability with a decreasing effect of species wood density, diameter, site and competition. Our results show that even if low, site effect can result from different soil nutrients depending on both tree size and species wood density. We observed higher tree growth on sites with (i) high exchangeable K, organic C, total N and total P for low wood density species; (ii) high available P and C:N for small trees, high exchangeable Ca and Mg for medium to large trees, all belonging to medium and hard wood density species. At the population level, the best models explained between 0 to 43% of growth variability, with significant competition effect (resp. site effect) for 21 (resp. 9) of the 43 species studied. Site ranking varied greatly between the 9 species concerned, probably reflecting different sensitivities to the scarcity of particular soil nutrients. 4. Synthesis. Our study provides original results on the factors influencing tree growth in Central Africa, showing t

Coupling plant growth model with pests and diseases (P&D) models, with consideration for the long-term feedback that occurs after the interaction, is still a challenging task nowadays. While a number of studies have examined various methodologies, none of them provides a generic frame able to host existing models and their codes without updating deeply their architecture. We developed MIMIC (Mediation Interface for Model Inner Coupling), an open-access framework/tool for this objective. MIMIC allows to couple plant growth and P&D models in a variety of ways. Users can experiment with various interaction configurations, ranging from a weak coupling that is mediated by the direct exchange of inputs and outputs between models to an advanced coupling that utilizes a third-party tool if the models' data or operating cycles do not align. The users decide how the interactions operate, and the platform offers powerful tools to design key features of the interactions, mobilizing metaprogramming techniques. The proposed framework is demonstrated, implementing coffee berry borers' attacks on Coffea arabica fruits. Observations conducted in a field in Sumatra (Indonesia) assess the coupled interaction model. Finally, we highlight the user-centric implementation characteristics of MIMIC, as a practical and convenient tool that requires minimal coding knowledge to use.

Increasing yield resiliency under water deficits remains a high priority for crop improvement. In considering the yield benefit of a plant trait modification, two facts are often overlooked: (1) the total amount of water available to a crop through a growing season ultimately constrains growth and yield cannot exceed what is possible with the limited amount of available water, and (2) soil water content always changes over time, so plant response needs to be considered within a temporally dynamic context of day-to-day variation in soil water status. Many previous evaluations of drought traits have implicitly considered water deficit from a “static” perspective, but while the static approach of stable water deficit treatments is experimentally congruous, the results are not realistic representations of real-world drought conditions, where soil water levels are always changing. No trait always results in a positive response under all drought scenarios. In this paper, we suggest two key traits for improving grain legume yield under water deficit conditions: (1) partial stomata closure at elevated atmospheric vapor pressure deficit that results in soil water conservation, and (2) lessening of the high sensitivity of nitrogen fixation activity to soil drying.

Introduction: The Family of pathogenesis-related proteins 10 (PR-10) is widely distributed in the plant kingdom. PR-10 are multifunctional proteins, constitutively expressed in all plant tissues, playing a role in growth and development or being induced in stress situations. Several studies have investigated the preponderant role of PR-10 in plant defense against biotic stresses; however, little is known about the mechanisms of action of these proteins. This is the first systematic review conducted to gather information on the subject and to reveal the possible mechanisms of action that PR-10 perform. Methods: Therefore, three databases were used for the article search: PubMed, Web of Science, and Scopus. To avoid bias, a protocol with inclusion and exclusion criteria was prepared. In total, 216 articles related to the proposed objective of this study were selected. Results: The participation of PR-10 was revealed in the plant's defense against several stressor agents such as viruses, bacteria, fungi, oomycetes, nematodes and insects, and studies involving fungi and bacteria were predominant in the selected articles. Studies with combined techniques showed a compilation of relevant information about PR-10 in biotic stress that collaborate with the understanding of the mechanisms of action of these molecules. The up-regulation of PR-10 was predominant under different conditions of biotic stress, in addition to being more expressive in resistant varieties both at the transcriptional and translational level. Discussion: Biological models that have been proposed reveal an intrinsic network of molecular interactions involving the modes of action of PR-10. These include hormonal pathways, transcription factors, physical interactions with effector proteins or pattern recognition receptors and other molecules involved with the plant's defense system. Conclusion: The molecular networks involving PR-10 reveal how the plant's defense response is mediated, either to trigger sus

Plant ecologists and molecular biologists have long considered the hypothesis of a trade-off between plant growth and defence separately. In particular, how genes thought to control the growth-defence trade-off at the molecular level relate with trait-based frameworks in functional ecology, such as the slow-fast plant economics spectrum, is unknown. We grew 49 phenotypically diverse rice genotypes in pots under optimal conditions, and we measured growth-related functional traits and the constitutive expression of 11 genes involved in plant defence. We also quantified the concentration of silicon (Si) in leaves to estimate silica-based defences. Rice genotypes were aligned along a slow-fast continuum, with fast-growing, early-flowering genotypes vs late-flowering, slow-growing genotypes. Leaf dry matter content and leaf Si concentrations were not aligned with this axis and negatively correlated with each other. Live-fast genotypes exhibited greater expression of OsNPR1, a regulator of the salicylic acid pathway that promotes plant defence while suppressing plant growth. These genotypes also exhibited greater expression of SPL7 and GH3.2 that are also involved in both stress resistance and growth. Our results do not support the hypothesis of a growth-defence trade-off when leaf Si and LDMC are considered, but they do when hormonal pathway genes are considered. We demonstrate the benefits of combining ecological and molecular approaches to elucidate the growth-defence trade-off, opening new avenues for plant breeding and crop science.

Heat and drought stresses have become more frequent and intense in the Mediterranean, strongly influencing arable crop phenology, growth, and grain yield. Agroforestry systems can effectively buffer the adverse climate conditions and stabilize or even increase crop yield under climate change. However, the positive effects of agroforestry remain uncertain due to the possible intense competition between trees and crops, especially for legume crops that have been less studied than cereals in such context. This study aimed to assess the response of the phenology, growth, grain yield, and yield-related traits of chickpea (Cicer arietinum), faba bean (Vicia faba), and lentil (Lens culinaris) to olive-based agroforestry (AFS) as compared to sole crops system (SCS) in the South of the Mediterranean. We conducted a field experiment during two growing seasons marked by an intense drought, either at the beginning (year 1) or at the end (year 2) of the crop cycle. Crop growth and yield were lower in year 1 than in year 2, reflecting the adverse growing conditions caused by the early drought. They were also lower in AFS than in SCS for both years, indicating that trees had competitive effects on crops. In year 1, the yield loss of grains in AFS was 66 % for lentil, 47 % for chickpea, and 43 % for faba bean compared to SCS, confirming the greater shade sensitivity of lentil. In year 2, the reduction was significantly smaller and was about 46 %, 34 %, 38 % for lentil, chickpea and, faba bean, respectively. The number of pods and grains were the most affected yield components by agroforestry and drought timing across the three legumes crops. Similar responses were found when comparing crops at different distances to trees within the AFS field. Crops generally had lower biomass and yield, explained by fewer pods and grains, on the northern side of trees compared to the southern side of trees or the middle of tree inter-rows, causing significant spatial heterogeneity in crops. Howeve

Background: Plants have adapted to survive seasonal life-threatening frost and drought. However, the timing and frequency of such events are impacted by climate change, jeopardizing plant survival. Understanding better the strategies of survival to dehydration stress is therefore timely and can be enhanced by the cross-fertilization of research between disciplines (ecology, physiology), models (woody, herbaceous species) and types of stress (drought, frost). Scope: We build upon the 'growth–stress survival' trade-off, which underpins the identification of global plant strategies across environments along a 'fast–slow' economics spectrum. Although phenological adaptations such as dormancy are crucial to survive stress, plant global strategies along the fast–slow economic spectrum rarely integrate growth variations across seasons. We argue that the growth–stress survival trade-off can be a useful framework to identify convergent plant ecophysiological strategies to survive both frost and drought. We review evidence that reduced physiological activity, embolism resistance and dehydration tolerance of meristematic tissues are interdependent strategies that determine thresholds of mortality among plants under severe frost and drought. We show that complete dormancy, i.e. programmed growth cessation, before stress occurrence, minimizes water flows and maximizes dehydration tolerance during seasonal life-threatening stresses. We propose that incomplete dormancy, i.e. the programmed reduction of growth potential during the harshest seasons, could be an overlooked but major adaptation across plants. Quantifying stress survival in a range of non-dormant versus winter- or summer-dormant plants, should reveal to what extent incomplete to complete dormancy could represent a proxy for dehydration tolerance and stress survival. Conclusions: Our review of the strategies involved in dehydration stress survival suggests that winter and summer dormancy are insufficiently acknowledged

We hypothesized that the nitrogen-fixing tree Acacia mangium could improve the growth and nitrogen nutrition of non-fixing tree species such as Eucalyptus. We measured the N-mineralization and respiration rates of soils sampled from plots covered with Acacia, Eucalyptus or native vegetation at two tropical sites (Itatinga in Brazil and Kissoko in the Congo) in the laboratory. We used a bioassay to assess N bioavailability to eucalypt seedlings grown with and without chemical fertilization for at least 6 months. At each site, Eucalyptus seedling growth and N bioavailability followed the same trends as the N-mineralization rates in soil samples. However, despite lower soil N-mineralization rates under Acacia in the Congo than in Brazil, Eucalyptus seedling growth and N bioavailability were much greater in the Congo, indicating that bioassays in pots are more accurate than N-mineralization rates when predicting the growth of eucalypt seedlings. Hence, in the Congo, planting Acacia mangium could be an attractive option to maintain the growth and N bioavailability of the non-fixing species Eucalyptus while decreasing chemical fertilization. Plant bioassays could help determine if the introduction of N2-fixing trees will improve the growth and mineral nutrition of non-fixing tree species in tropical planted forests.

La couronne d'un arbre résulte de l'apparition répétée d'éléments végétatifs (métamères, axes) et reproducteurs, et de leur disparition. L'apparition et la disparition de ces éléments sont conditionnées par des facteurs architecturaux, environnementaux et d'éventuelles manipulations humaines. En particulier, la taille des arbres fruitiers est connue pour stimuler le débourrement des bourgeons et la croissance végétative. Le manguier est un arbre fruitier tropical d'intérêt économique, culturel et nutritionnel dont la culture pose des problèmes agronomiques majeurs, tels que des rendements en général faibles et irréguliers d'une année sur l'autre, et des asynchronismes phénologiques qui conduisent à la présence dans les vergers de stades sensibles aux bioagresseurs sur de longues périodes. Ces contraintes proviennent en partie des interactions fortes entre croissance végétative et reproduction qui ont été montrées chez cette espèce. Ainsi, l'importance et la dynamique de la croissance végétative précédant la floraison déterminent en grande partie le succès de cette dernière. Le modèle structure-fonction d'élaboration du rendement et de la qualité de la mangue V-Mango est construit sur ces connaissances, et a pour vocation la conception assistée par modèle d'itinéraires techniques plus durables, basés notamment sur une optimisation de la taille. Cette pratique a été récemment intégrée au modèle. Cependant, les simulations montrent une surestimation de la croissance végétative et de la production, liée à une surproduction de nouveaux axes et à la non prise en compte de la mortalité des axes. Nous faisons l'hypothèse que la lumière, en tant que source d'énergie et signal, a un rôle essentiel dans les processus de débourrement végétatif, de chute des feuilles et de mortalité des axes du manguier, et qu'intégrer ces effets dans V-Mango peut conduire à une amélioration significative des sorties du modèle. Pour évaluer cette hypothèse, nous avons quantifié la variabilité de

Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across the sub-Saharan Sahel region. Pearl millet's early root system features a single fast-growing primary root which we hypothesize is an adaptation to the Sahelian climate. Using crop modeling, we demonstrate that early drought stress is an important constraint in agrosystems in the Sahel where pearl millet was domesticated. Furthermore, we show that increased pearl millet primary root growth is correlated with increased early water stress tolerance in field conditions. Genetics including genome-wide association study and quantitative trait loci (QTL) approaches identify genomic regions controlling this key root trait. Combining gene expression data, re-sequencing and re-annotation of one of these genomic regions identified a glutaredoxin-encoding gene PgGRXC9 as the candidate stress resilience root growth regulator. Functional characterization of its closest Arabidopsis homolog AtROXY19 revealed a novel role for this glutaredoxin (GRX) gene clade in regulating cell elongation. In summary, our study suggests a conserved function for GRX genes in conferring root cell elongation and enhancing resilience of pearl millet to its Sahelian environment.

Cupuassu (Theobroma grandiflorum [Willd. ex Sprengel] Schumann) seeds constitute the raw material for oil extraction and fabrication of cupulate (product similar to chocolate). However, fungal diseases such as witches' broom caused by Moniliophthora perniciosa have interfered with the large-scale development of cupuassu plantations. Cupuassu genetic breeding programmes focus on a variety of biotechnological tools or approaches to select genes related to quality or resistance mechanisms. In this study, we used expression and interactomics analyses of preselected genes involved in fruit quality and/or resistance to better understand the molecular and physiological mechanisms associated with these plant processes. It was found that (i) resistant and susceptible cupuassu genotypes showed different pulp characteristics as well as gene expression patterns; (ii) monosaccharide and carbohydrate transport pathways were enhanced during fruit maturation; (iii) sugar accumulation participated in signal transduction associated with fruit development and stress response in maturing fruits; and (iv) maturing pulp and seeds showed increased phospholipid metabolism and translocation, as well as immune system activation. The TgSTP1, TgWRKY33, TgCZF1, and TgUBA1 genes in cupuassu and the orthologues of DIN10, CNI1, and TET8 identified by the interactomics approach may be good candidates for marker-assisted selection in breeding programmes focusing on both fruit quality and resistance/tolerance to biotic/abiotic stress.

The influence of soaking and germination on the physicochemical characteristics of tigernut tubers (Cyperus esculentus L.) are investigated. Tubers (Ø > 1 cm) were soaked in an ascorbic acid solution (0.1%) for 48 h at 40 °C before undergoing germination for 6 days. The textural profile, the capacity and germination energy and the biochemical composition of these tubers were determined. The germination energy varied from 76.18 to 79.48% for the quantities of solution of 24 and 48 mL, respectively. The textural profile of the tubers varied depending on the type of treatment. Germination resulted in a considerable reduction in amylose content from 14.15% for the native tigernut tuber to 9.98% for the sprouted one. This treatment also increased the protein, ascorbic acid and ash contents, which ranged from 7.54 to 8.82 g/100 g DM, 250 to 275.39 mg/100 g DM and 2.60 to 3.84 g/100 g DM, respectively. The starch content of the tubers following germination remained high, which could come up against the pasteurization of the milk from these tubers.

Sexual differentiation of inflorescences and flowers is important for reproduction and affects crop plant productivity. We report here on a molecular study of the process of sexual differentiation in the immature inflorescence of oil palm (Elaeis guineensis). This species is monoecious and exhibits gender diphasy, producing male and female inflorescences separately on the same plant in alternation. Three main approaches were used: small RNA-seq to characterise and study the expression of miRNA genes; RNA-seq to monitor mRNA accumulation patterns; hormone quantification to assess the role of cytokinins and auxins in inflorescence differentiation. Our study allowed the characterisation of 30 previously unreported palm MIRNA genes. In differential gene and miRNA expression studies, we identified a number of key developmental genes and miRNA-mRNA target modules previously described in relation to their developmental regulatory role in the cereal panicle, notably the miR156/529/535-SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) gene regulatory module. Gene enrichment analysis highlighted the importance of hormone-related genes, and this observation was corroborated by the detection of much higher levels of cytokinins in the female inflorescence. Our data illustrate the importance of branching regulation within the developmental window studied, during which the female inflorescence, unlike its male counterpart, produces flower clusters on new successive axes by sympodial growth.

Tree growth and mortality are two central processes in mixed and structurally complex moist tropical forests, yet accurate estimates of the variables needed to model them remain sparse and scattered. It is thus still difficult to predict forest evolution at a local scale and build reliable management plans. To help fill this gap, for 1–7 years we annually monitored 21,180 trees belonging to 42 species exploited for timber production in Central Africa. We made new species-specific estimates of diameter increments and mortality rates, and investigated how tree growth varied with tree size and logging history. We compared our results with the legal values of diameter increments, mortality rates, and minimum cutting diameters used to build forest management plans in Cameroon. Diameter increment was found to vary with tree size for most of the species studied. The relationships between diameter increment and tree size were mostly humpback-shaped. The trees with diameters close to or lower than the reference minimum cutting diameter generally grew faster than the average. We also found that tree growth could slow for 1–2 years after timber exploitation and was then spurred for at least 5 years. The tree growth response to logging was nevertheless species-specific. This study provides new estimates of tree diameter increments and mortality rates that could help make more accurate forest projections and draw up sustainable management plans in Africa.

Plants exhibit plasticity in response to various external conditions, characterized by changes in physiological and morphological features. Although being non-negligible, compared to the other environmental factors, the effect of wind on plant growth is less extensively studied, either experimentally or computationally. This study aims to propose a modeling approach that can simulate the impact of wind on plant growth, which brings a biomechanical feedback to growth and biomass distribution into a functional–structural plant model (FSPM). Tree reaction to the wind is simulated based on the hypothesis that plants tend to fit in the environment best. This is interpreted as an optimization problem of finding the best growth-regulation sink parameter giving the maximal plant fitness (usually seed weight, but expressed as plant biomass and size). To test this hypothesis in silico, a functional–structural plant model, which simulates both the primary and secondary growth of stems, is coupled with a biomechanical model which computes forces, moments of forces, and breakage location in stems caused by both wind and self-weight increment during plant growth. The Non-dominated Sorting Genetic Algorithm II (NSGA-II) is adopted to maximize the multi-objective function (stem biomass and tree height) by determining the key parameter value controlling the biomass allocation to the secondary growth. The digital trees show considerable phenotypic plasticity under different wind speeds, whose behavior, as an emergent property, is in accordance with experimental results from works of literature: the height and leaf area of individual trees decreased with wind speed, and the diameter at the breast height (DBH) increased at low-speed wind but declined at higher-speed wind. Stronger wind results in a smaller tree. Such response of trees to the wind is realistically simulated, giving a deeper understanding of tree behavior. The result shows that the challenging task of modeling plant plas

Dans la zone soudano sahélienne d'Afrique de l'Ouest, la productivité agricole est fortement affectée par la variabilité et les changements climatiques. La production agricole est dominée par la production pluviale de céréales telles que le maïs, le mil et le sorgho, pour la consommation alimentaire. Les agriculteurs ont des rendements faibles et variables, ce qui entraîne une incertitude croissante quant à leur capacité à produire davantage pour nourrir une population en forte croissance. L'objectif général de cette thèse était de concevoir des systèmes de culture plus productifs et stables, adaptés au changement climatique, en explorant les bénéfices de l'association sorgho-niébé, combiné a des choix contrastés de variété de sorgho, de fertilisation minérale et de date de semis. L'approche était basée sur un travail d'enquête, d'expérimentation au champ et de simulation à l'aide d'un modèle de culture, pour un cas d'étude au centre du Mali en Afrique de l'Ouest. Une première étape a porté sur l'identification de la perception du changement climatique par les agriculteurs et les stratégies d'adaptation agricole qu'ils considèrent pertinentes pour faire face à la variabilité et au changement climatique. En second lieu, le modèle de culture STICS a été calibré sur la base de deux années d'expérimentation (2017, 2018) de la culture associée sorgho-niébé à la station agronomique de N'Tarla. Dans ce dispositif expérimental, deux variétés de sorgho (locale et améliorée) avec une sensibilité contrastée à la photopériode ont été étudiées en culture pure et en culture associée avec le niébé. Deux dates de semis et deux niveaux de fertilisation minérale ont également été étudiés.

Several abiotic factors influence the chemical composition of essential oils. Understanding these factors is an important step in developing quality products that meet market demands. This research work aims to study the chemical composition of the essential oils of ylang-ylang (Cananga odorata, forma genuina) according to the maturity of flowers. The volatile compounds of ylang-ylang flowers from Reunion Island were extracted by hydrodistillation and the samples were analyzed by high resolution gas chromatography coupled with mass spectrometry (GC-MS) allowing for the identification of 70 volatile compounds. The chemical composition of the essential oils extracted from the flowers at 5 different stages of development varies both qualitatively and quantitatively. The volatile compounds observed belong to 4 chemical groups which are esters, alcohols, terpenes, and ether-oxides. The synthesis of light oxygenated compounds is largely the predominant chemical subgroup in all stages of development. Their relative content is considerably increased during flower ripening with a peak concentration in stage 4 (SD4). The highest concentrations of non-terpene esters and heavy oxygenated compounds are found in stages 1 (SD1) and (SD5), respectively, while no chemical subgroup is dominant in the intermediate stages 2 (SD2) and (SD3). The dynamics of volatile compounds have also been studied. This study established that the stage of development of ylang-ylang flowers significantly influences the dynamics of volatile compounds in the extracted essential oils. Total oxygenated compounds that are highly odoriferous in essential oils increase progressively and significantly with flower maturity, unlike hydrocarbon terpenes, which are less valuable in terms of their contribution to the fragrance and following opposite kinetics; suggesting that odoriferous properties increase with the development of the flower, with a higher intensity at SD4.

Fin 2017, un observatoire de la Croissance de l'Herbe (OCH) a été mis en place sur l'île de la Réunion pour suivre la dynamique de la production d'herbe à l'aide de mesures hebdomadaires de la hauteur du couvert herbacé, dans un réseau de parcelles représentatives de la variabilité pédoclimatique de l'île. Conjointement, un suivi de la densité de l'herbe a été effectué afin de produire les références nécessaires à la conversion de la hauteur d'herbe en biomasse disponible. La densité moyenne est de 320 (± 32) kg de MS /cm /ha. Les densités les plus faibles sont observées durant la saison humide, de mars à avril, lorsque la croissance de l'herbe est forte, et les plus élevées en saison sèche de juillet à décembre, lorsque la croissance est ralentie. La densité est plus élevée en présence d'un tapis de stolons, et lorsque la hauteur d'herbe est inférieure à 13 cm ou lorsque le taux de MS du couvert herbacé augmente. La proportion de kikuyu dans le couvert herbacé n'a pas d'influence significative sur la densité. A la lumière des facteurs influants, un tableau multi-entrées de la densité de l'herbe a été élaboré. Cette grille de densité va permettre aux utilisateurs d'évaluer la production d'herbe avec plus de précision, et de produire et transférer en temps réel aux éleveurs les indicateurs d'aide à la gestion du pâturage (stock d'herbe disponible, nombre de jours d'avance, chargement animal optimal) pour un conseil agricole contextualisé.

En 2017, l'Association Réunionnaise de Pastoralisme et le CIRAD ont mis en place un observatoire de la croissance de l'herbe couvrant les trois principales zones de pâturage de l'île de la Réunion. Ce dispositif vise à mieux appréhender la dynamique saisonnière de la pousse de l'herbe, dans des secteurs où les profils de croissance s'avèrent très variables. Au sein de ce réseau, les niveaux de croissance variaient de 17 à 200 kg MS /ha/an au cours de l'année. Les premières courbes obtenues confirment l'existence de deux saisons de pousse contrastées : une période de forte croissance, de décembre à mars, et une période de croissance plus faible, de mai à septembre. Les trois premières années de suivi se caractérisent également par des années climatiques très contrastées, qui diffèrent des normales saisonnières. Ce suivi sera poursuivi, dans l'optique de mieux appréhender la variabilité intra et interannuelle, en lien avec les conditions météorologiques. Ces premiers résultats permettront de revoir les principaux indicateurs utilisés pour la gestion du pâturage. Ainsi, le niveau de chargement global sera revu à la hausse, et les surfaces offertes par vache seront mieux ajustés au cours de la saison. Un travail de transfert technique a déjà été mis en oeuvre, à travers l'édition d'un bulletin mensuel et l'organisation d'animations de terrain à destination des éleveurs et techniciens.

In coffee, fruit production on a given shoot drops after some years of high yield, triggering pruning to induce resprouting. The timing of pruning is a crucial farmer's decision affecting yield and labour. One reason for fruit production drop could be the exhaustion of resources, particularly the non-structural carbohydrates (NSC). To test this hypothesis in a Coffea L. arabica agroforestry system, we measured the concentrations of NSC, carbon (C) and nitrogen (N) in leaves, stems and stumps of the coffee plants, 2 and 5 years after pruning. We also compared shaded vs full sun plants. For that purpose, both analytical reference and visible and near infrared reflectance spectroscopy (VNIRS) methods were used. As expected, concentrations of biochemical variables linked to photosynthesis activity (N, glucose, fructose, sucrose) decreased from leaves to stems, and then to stumps. In contrast, variables linked more closely to plant structure and reserves (total C, C:N ratio, starch concentration) were higher in long lifespan organs like stumps. Shading had little effect on most measured parameters, contrary to expectations. Concentrations of N, glucose and fructose were higher in 2-year-old organs. Conversely, starch concentration in perennial stumps was three times higher 5 years after pruning than 2 years after pruning, despite high fruit production. Therefore, the drop in fruit production occurring after 5–6 years was not due to a lack of NSC on plant scale. Starch accumulation in perennial organs concurrently to other sinks, such as fruit growth, could be considered as a 'survival' strategy, which may be a relic of the behaviour of wild coffee (a tropical shade-tolerant plant). This study confirmed that VNIRS is a promisingly rapid and cost-effective option for starch monitoring (coefficient of determination for validation, R2 val = 0.91), whereas predictions were less accurate for soluble sugars, probably due to their too similar spectral signature.

Les enfants élaborent des représentations de la science et des scientifiques dès les premières années de l'école primaire. À ce niveau scolaire, les élèves qui participent à des activités expérimentales, encadrées par des enseignants et des scientifiques, bénéficient d'un autre regard sur la recherche et se projettent davantage dans les pratiques scientifiques. Avec l'appui du Centre de recherches interdisciplinaires, de la Cité éducative de Creil, et l'intervention d'un chercheur en science du bois, plusieurs classes des écoles élémentaires du Réseau d'éducation prioritaire de Creil se sont intéressées à la croissance des arbres, à la faveur d'un projet proposé par le dispositif " Savanturiers ". En se conformant à une séquence scientifique usuelle (observations, questionnements, bibliographies, élaboration des protocoles de recherche, recherche proprement dite, analyses et interprétations, conclusion et restitution), compatible avec les activités d'apprentissage en classe, enseignants et élèves se sont intéressés à la croissance des arbres. Leurs recherches se sont focalisées sur le rôle de la sève et de la résine dans l'arbre, mais aussi sur les manières dont les humains et d'autres êtres vivants peuvent transformer et valoriser ces substances naturelles produites par les arbres au cours de leur croissance. En combinant la recherche documentaire et l'appui pédagogique des enseignants, d'un chercheur et d'une guide nature, tout en favorisant le dialogue entre les élèves et ces intervenants, ce projet pédagogique innovant a rendu les élèves acteurs de leur propre apprentissage de la nature. Outre l'aspect scientifique lié à la croissance des arbres, les jeunes élèves ont développé leur curiosité, leur rigueur, leur esprit créatif et critique, notamment à la faveur d'une journée d'immersion en forêt et du suivi de très jeunes arbres en salle de classe. Enfin, les élèves ont su relier les connaissances acquises au cours de ce projet de pratique de la recherche à des

The purpose of this investigation was to assess the nodulation and the plant growth of white lupin (Lupinus albus L.) in Tunisian soils. For this, two L. albus varieties, Mekna (Tunisian) and Lumen (French), were cultivated in soils sampled from 56 locations without a history of lupin cultivation or rhizobial inoculation. All soils were analyzed for their physical and chemical proprieties. At harvest, nodulation and plant growth parameters were recorded for both varieties. The results showed that 75% of soils were alkaline and calcareous. Nodulation was often poor, suggesting the absence of host-specific rhizobia. It was absent in 41 soils, abundant in 2 (Sejnen and Mraissa), and scarce but efficient in the remaining 13 soils. Furthermore, white lupin varieties have different responses to native nodulating bacteria since the local variety, Mekna, developed significantly more root nodules and in more soils than the imported variety. Growth parameters and nitrogen accumulation were also measured as indicators of nitrogen fixation efficiency. Considerable variability was detected among soils. It was found that the imported variety, Lumen, showed high sensitivity to the soil active lime content compared to Mekna. Interestingly, high biomass production and nitrogen accumulation were recorded in soils originating from Madian, Tejerouin, Benouria, and Oued zinga, with a high active lime content ranging from 26% to 31%. This finding suggested that white lupin can be cultivated in Tunisian calcareous soils, especially the most promising local variety, Mekna. Isolation and the selection of efficient bacteria from alkaline calcareous soils are necessary to promote the development of this valuable legume in Tunisia.

The occurrence of natural root grafts, the union of roots of the same or different trees, is common and shared across tree species. However, their significance for forest ecology remains little understood. While early research suggested negative effects of root grafting with the risk of pathogen transmission, recent evidence supports the hypothesis that it is an adaptive strategy that reduces stress by facilitating resource exchange. Here, by analysing mangrove root graft networks in a non-destructive way at stand level, we show further evidence of cooperation-associated benefits of root grafting. Grafted trees were found to dominate the upper canopy of the forest, and as the probability of grafting and the frequency of grafted groups increased with a higher environmental stress, the mean number of trees within grafted groups decreased. While trees do not actively 'choose' neighbours to graft to, our findings point to the existence of underlying mechanisms that regulate 'optimal group size' selection related to resource use within cooperating networks. This work calls for further studies to better understand tree interactions (i.e. network hydraulic redistribution) and their consequences for individual tree and forest stand resilience.

The physiological stage of mango maturity at harvest affects fruit quality at the time it is commercialized. The objective assessment of the mango maturity stage at harvest remains a challenge because of the multiple interactions between the visual aspect, the physicochemical composition, and the morphology of the fruit. This study aims to quantify the optimal harvest date to guarantee the maturity and quality of mango cv. 'Kent'. In this study, which took place in Korhogo in northern Côte d‟Ivoire, the maturity stage and the quality of fruits were measured on 240 Kent mangoes harvested at four different harvest dates (90, 95, 100, and 105 days after flowering). A morphological, physical, and chemical characterization (length, width, mass, soluble sugar content, dry matter, skin appearance, and mass loss) was carried out at each date on 30 fruits at harvest and 30 fruits after ripening at room temperature. The number of days necessary for ripening was quantified. The results showed that the optimal harvest date is 100 days after flowering for Kent mangoes in Côte d'Ivoire. Mangoes harvested at this date developed better organoleptic characteristics (less wrinkled skin, lower mass losses, and a shorter ripening time). Assessment of the harvest date, guaranteeing better ripening of the fruit, is of utmost importance for organizing harvests in the mango sectors in West Africa, and especially in Côte d'Ivoire.

This study consists of the development of tree growth models to deduce stands productivity and determine the highest productive species in the conditions of the concerned plantation. Seven Eucalyptus, introduced in the arboretum of Souiniet (north-west of Tunisia, wet Mediterranean bioclimate) in a Cork Oak natural forest, were studied. Stem analysis and non-linear growth modeling regression equations were used to predict wood productivities. Gompertz and Chapman–Richards growth function appeared as being great numerical models to estimate the Eucalyptus tree diameter and height evolutions, respectively. Moreover, an adapted Chapman–Richards model allowed predicting the volume of trees in an efficient manner. The values of mean annual volume productivity of the Eucalyptus spp.studied, allow us to classify them in order of increasing annual productivity, as follows: E. sideroxylon, E. cinerea, E. maidenii, E. macrorhyncha, E. tereticornis, E. viminalis and E. bicostata. The first three Eucalyptus spp. appeared as the best-adapted and most suitable Eucalyptus trees for new plantations in this area. These species had the highest mean annual increments, ranged from 5 to 10 m3.ha−1.year−1 with 15 to 20 years of rotation. E. bicostata is the most promising, with annual average production exceeding 10 m3.ha−1.year−1 after 25 years, and reaching 20 m3.ha−1.year−1 at 40 years old. These modeling approaches provide additional knowledge on the productivity of the different Eucalyptus species, thus enabling forestry operators to simulate the development of forest stands in order to optimize timber production and harvesting.

In response to gravistimulation under anisotropic light, tree stems showing an active cambium produce reaction wood that redirects the axis of the trees. Several studies have described transcriptomic or proteomic models of reaction wood relative to the opposite wood. However, the mechanisms leading to the formation of reaction wood are difficult to decipher because so many environmental factors can induce various signalling pathways leading to this developmental reprogramming. Using an innovative isotropic device where the phototropic response does not interfere with gravistimulation we characterized the early molecular responses occurring in the stem of poplar after gravistimulation in an isotropic environment, and without deformation of the stem. After 30 min tilting at 35° under anisotropic light, we collected the upper and lower xylems from the inclined stems. Controls were collected from vertical stems. We used a microarray approach to identify differentially expressed transcripts. High-throughput real-time PCR allowed a kinetic experiment at 0, 30, 120 and 180 min after tilting at 35°, with candidate genes. We identified 668 differentially expressed transcripts, from which we selected 153 candidates for additional Fluidigm qPCR assessment. Five candidate co-expression gene clusters have been identified after the kinetic monitoring of the expression of candidate genes. Gene ontology analyses indicate that molecular reprogramming of processes such as 'wood cell expansion', 'cell wall reorganization' and 'programmed cell death' occur as early as 30 min after gravistimulation. Of note is that the change in the expression of different genes involves a fine regulation of gibberellin and brassinosteroid pathways as well as flavonoid and phosphoinositide pathways. Our experimental set-up allowed the identification of genes regulated in early gravitropic response without the bias introduced by phototropic and stem bending responses.