26 results on '"Fabio Canal"'
Search Results
2. MLH1 Deficiency Down-Regulates TLR4 Expression in Sporadic Colorectal Cancer
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Melania Scarpa, Cesare Ruffolo, Andromachi Kotsafti, Fabio Canal, Francesca Erroi, Silvia Basato, Lucia Dall’Agnese, Alain Fiorot, Anna Pozza, Paola Brun, Nicolò Bassi, Angelo Dei Tos, Carlo Castoro, Ignazio Castagliuolo, and Marco Scarpa
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colorectal cancer ,TLR4 ,MLH1 ,mismatch ,innate immunity ,Biology (General) ,QH301-705.5 - Abstract
Patients with mismatch repair (MMR)-deficient colorectal cancer (CRC) have a more favorable prognosis than patients with tumors with intact MMR. In order to obtain further insights on the reasons for this different outcome, we investigated the interplay between MMR genes and TLR4/MyD88 signaling. The cancer genome atlas (TCGA) databases were selected to predict the differential expression of TLR4 in colon cancer and its correlation with MMR genes. Moreover, the expression of MMR genes and TLR4 was evaluated by immunohistochemistry in 113 CRC samples and a cohort of 63 patients was used to assess TLR4 mRNA expression and MLH1 epigenetic silencing status. In vitro, the effect of MLH1 knockdown on TLR4 expression was quantified by Real Time PCR. TLR4 expression resulted dependent on MMR status and directly correlated to MLH1 expression. In vitro, MLH1 silencing decreased TLR4 expression. These observations may reflect the better prognosis and the chemoresistance of patients with CRC and MMR defects.
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- 2021
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3. Colorectal polypoid lesions and expression of vascular endothelial growth factor in a consecutive series of endoscopic and surgical patients
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Cesare Ruffolo, Luisa Toffolatti, Fabio Canal, Andromachi Kotsafti, Giulia Pagura, Anna Pozza, Marta Campo Dell’Orto, Francesco Ferrara, Marco Massani, Angelo P Dei Tos, Carlo Castoro, Nicolò Bassi, and Marco Scarpa
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Colorectal cancer incidence in patients undergoing screening protocols is decreasing because of the higher rate of discovered preneoplastic colonic lesions; however, adenomatous polyps may not always be removable endoscopically and surgery may still be necessary. The aim of this study was to assess the vascular endothelial growth factor expression in the different steps of colorectal carcinogenesis to explore its potential role as a marker of malignancy in polypoid lesions. A total of 92 subjects with colonic adenoma or cancer who underwent screening colonoscopy or surgery were prospectively enrolled. Real-time reverse transcription polymerase chain reaction for VEGF-A messenger RNA expression and immunohistochemistry for VEGF-A were performed. Immunoassays for VEGF-A, VEGF-C, VEGFR-1, VEGFR-2, and VEGFR-3 were also performed. Non-parametric statistics, receiver operating characteristic curve analysis, and logistic multiple regression analysis were used. VEGF-A messenger RNA expression was higher in patients with high-grade dysplasia or colorectal cancer than in those with low-grade dysplasia adenomas (p = 0.01). At immunohistochemistry, VEGF-A expression was significantly higher in colorectal cancer patients compared to dysplastic adenomas (p
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- 2017
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4. Prognostic Significance of Circulating and Endothelial Progenitor Cell Markers in Type 2 Diabetic Foot
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Maria Sambataro, Elena Seganfreddo, Fabio Canal, Anna Furlan, Laura del Pup, Monia Niero, Agostino Paccagnella, Filippo Gherlinzoni, and Angelo Paolo dei Tos
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Objective. We studied circulating precursor cells (CPC) in type 2 diabetes mellitus (T2DM) with neuropathic foot lesions with or without critical limb ischemia and relationships between endothelial precursor cells (EPC) and peripheral neuropathy. Methods and Subjects. We measured peripheral blood CD34, CD133, and CD45 markers for CPC and KDR, CD31 markers for EPC by citofluorimetry and systemic neural nociceptor CGRP (calcitonin gene related protein) by ELISA in 8 healthy controls (C) and 62 T2DM patients: 14 with neuropathy (N), 20 with neuropathic foot lesions (N1), and 28 with neuroischemic recent revascularized (N2) foot lesions. Timing of lesions was: acute (until 6 weeks), healed, and not healed. Results. CD34+ and CD133+ were reduced in N, N1, and N2 versus C, and CD34+ were lower in N2 versus N1 (P=0.03). In N2 CD34+KDR+ remain elevated in healed versus chronic lesions and, in N1 CD133+31+ were elevated in acute lesions. CGRP was reduced in N2 and N1 versus C (P
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- 2014
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5. The thyro-cricoarytenoid space (TCAS): clinical and prognostic implications in laryngeal cancer
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Federica Sovran, Fabio Canal, Marco Lucioni, Marco Lionello, Giuseppe Rizzotto, Andy Bertolin, and Francesco Guida
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Male ,medicine.medical_specialty ,glottic carcinoma ,tirocricoaritenoideo ,medicine.medical_treatment ,Kaplan-Meier Estimate ,Laryngology ,TCAS ,Surgical oncology ,medicine ,Humans ,Pathological ,Laryngeal Neoplasms ,posterior ,Aged ,Retrospective Studies ,Univariate analysis ,business.industry ,Cancer ,spazio ,space ,Middle Aged ,medicine.disease ,Prognosis ,Glottic Squamous Cell Carcinoma ,Laryngectomy ,thyrocricoarytenoid ,Survival Rate ,General Energy ,Otorhinolaryngology ,Glottic cancer ,Cohort ,Carcinoma, Squamous Cell ,Female ,Radiology ,posteriore ,Neoplasm Recurrence, Local ,business ,carcinoma glottico - Abstract
Lo spazio tiro-crico-aritenoideo (TCAS): implicazioni cliniche e prognostiche nel carcinoma laringeo.La recente letteratura riguardante la chirurgia oncologica laringea si sta sempre più focalizzando sul significato prognostico negativo del coinvolgimento da parte della neoplasia della porzione posteriore dello spazio paraglottico inferiore, che può essere definito spazio “tiro-crico-aritenoideo” (TCAS). Abbiamo valutato retrospettivamente il significato prognostico del coinvolgimento di tale sito anatomico in una coorte di 84 pazienti trattati con laringectomia parziale orizzontale open. È stato inoltre valutato mediante analisi univariata il significato prognostico dei parametri clinici e patologici. I casi con coinvolgimento del TCAS hanno avuto un maggior tasso di recidiva ed una minore sopravvivenza libera da malattia, rispetto ai casi senza coinvolgimento dello stesso. In conclusione, le neoplasie coinvolgenti questo sito laringeo dovrebbero essere considerate e trattate come tumori extra-laringei. I carcinomi glottici posteriori con invasione del TCAS hanno una prognosi peggiore quando gestiti mediante chirurgia conservativa. Nei casi di carcinoma glottico localmente avanzato con coinvolgimento del TCAS la laringectomia totale dovrebbe essere considerata il trattamento di scelta.The recent literature on laryngeal surgical oncology has increasingly focused on the negative prognostic impact of neoplastic involvement of the posterior portion of the inferior paraglottic space, which we refer to as the “thyro-cricoarytenoid space” (TCAS). We retrospectively considered the prognostic significance of TCAS involvement in a cohort of 84 patients treated with open partial horizontal laryngectomy for glottic squamous cell carcinoma. Univariate analysis was conducted on the prognostic value of several clinical and pathological parameters. Cases with TCAS involvement experienced a higher recurrence rate and shorter disease-free survival. Neoplasms involving the TCAS should be considered and treated as extralaryngeal malignancies. Posterior glottic tumours with TCAS invasion have worse prognosis when managed with conservative surgery. Total laryngectomy should be considered in cases of locally-advanced glottic carcinoma with TCAS involvement.
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- 2020
6. Assessment of Contrast-Enhanced Ultrasound (CEUS) and Computed Tomography (CT) diagnostic accuracy in the evaluation of challenging cystic renal masses
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Lorenzo Angelini, Eugenio Gioulis, Nicoletta Civitareale, Antonio Granata, Christian Zanza, Yaroslava Longhitano, Angelica Zago, Piernicola Machin, Fabio Canal, Armando Serao, Gianluca Piccoli, and Salvatore Valerio
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Internal Medicine ,Humans ,Contrast Media ,Radiology, Nuclear Medicine and imaging ,General Medicine ,Kidney Diseases, Cystic ,Tomography, X-Ray Computed ,Kidney ,Retrospective Studies ,Ultrasonography - Abstract
To assess the diagnostic accuracy of contrast-enhanced ultrasound (CEUS) and computed tomography (CT) within Bosniak IIF/III categories.After cystic renal mass diagnosis by contrast-enhanced CT, all patients with Bosniak score ≥ II also underwent CEUS between March 2017 and March 2019. Their exams were retrospectively analyzed. One experienced uro-radiologist performed every CEUS and reviewed the exams according to the EFSUMB 2020 Position Statement, while blinded to clinical data. CT Bosniak scores were retrospectively given blindly by two uro-radiologists (CT 1 and CT 2). We compared CEUS, CT 1 and CT 2 scores to clinical findings and histological tests. Clinical performance characteristics and area under the receiver operating characteristic (ROC) curves (AUCs) were determined separately for CEUS and CT, and then compared.101 cystic masses were analyzed. In Bosniak categories IIF and III, the AUCs were 0.854 for CT 1, 0.779 for CT 2, and 0.746 for CEUS.Despite some statistical limitations, this study confirms that among cystic renal masses, those classified as Bosniak IIF and III are the most difficult to assess. The diagnostic performances of CEUS and CT are similar within this group. However, in experienced hands, CEUS could be valuable in further evaluation of ambiguous cystic masses, and in more ductile, safer, and cost-effective surveillance of those classified as Bosniak IIF and III. When challenging cystic renal masses occur, CEUS is a useful tool for clinical management and for the follow-up of non-surgical lesions.
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- 2021
7. Could the infiltration of the thyroarytenoid muscle define the pT2 glottic carcinoma?
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Marco Lucioni, Marco Lionello, Giuseppe Rizzotto, Gino Marioni, Andy Bertolin, Ennio Nardello, Fabio Canal, and Luciano Giacomelli
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Male ,Glottis ,Microsurgery ,medicine.medical_specialty ,Cord ,Urology ,Disease ,Disease-Free Survival ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,otorhinolaryngologic diseases ,Clinical endpoint ,Humans ,Medicine ,Neoplasm Invasiveness ,Thyroarytenoid muscle ,Transoral laser microsurgery ,030223 otorhinolaryngology ,Laryngeal Neoplasms ,Pathological ,Aged ,Neoplasm Staging ,Retrospective Studies ,Univariate analysis ,business.industry ,Biopsy, Needle ,Middle Aged ,respiratory system ,Prognosis ,Immunohistochemistry ,Survival Analysis ,Treatment Outcome ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Cohort ,Carcinoma, Squamous Cell ,Female ,Laser Therapy ,Laryngeal Muscles ,business ,Vocal Cord Paralysis - Abstract
Background The involvement of the thyroarytenoid (TA) muscle by glottic cancer may be related to an impaired vocal cord mobility, which is classified as cT2 disease. The primary endpoint was to evaluate the prognostic significance of TA muscle involvement in early glottic cancer treated with transoral laser microsurgery (TLM). Methods A review was conducted on a cohort of 209 patients consecutively treated with TLM for early glottic carcinoma. Univariate analysis was used to examine the prognostic meaning of clinical and pathological parameters. Results The statistical analysis showed that TA muscle infiltration correlated significantly with a worse prognosis in terms of recurrence rate and disease-free survival, and this was confirmed even in the subcohort with pT1a glottic cancer. Conclusions Our preliminary findings suggest that it could be considered as a criterion for upstaging a glottic cancer from pT1 to pT2.
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- 2019
8. Laryngeal adenoid cystic carcinoma: Radical or conservative surgery?
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Marco Lionello, Giuseppe Rizzotto, Andy Bertolin, Raffaella Palumbo, Francesca Presotto, and Fabio Canal
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Adult ,Male ,medicine.medical_specialty ,Time Factors ,Adenoid cystic carcinoma ,medicine.medical_treatment ,Laryngectomy ,Conservative Treatment ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Radical surgery ,030223 otorhinolaryngology ,Surgical treatment ,Laryngeal Adenoid Cystic Carcinoma ,Laryngeal Neoplasms ,Partial laryngectomy ,Aged ,Retrospective Studies ,business.industry ,Margins of Excision ,Distant metastasis ,Middle Aged ,medicine.disease ,Carcinoma, Adenoid Cystic ,Surgery ,Survival Rate ,Treatment Outcome ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Female ,Larynx ,Neoplasm Recurrence, Local ,business ,Follow-Up Studies - Abstract
Purpose The present paper describes our experience in surgical treatment of laryngeal ACC, and discuss the effectiveness of conservative surgery. Methods We retrospectively reviewed the clinical charts of 17 patients with laryngeal ACC treated surgically at the Otolaryngology Unit of Vittorio Veneto Hospital (Italy) from November 1989 to April 2020. Results Fourteen patients underwent partial laryngectomy, and three had a total laryngectomy. Five patients (29%) experienced a laryngeal ACC relapse after a disease-free survival of 66.6 ± 50.1 months. The distant metastasis rate was 17%. At latest follow-up, two patients had died of distant metastatic disease after 156 and 243 months. Conclusions Radical surgery for laryngeal ACC does not warrant free margins and even cases with positive deep margins rarely experience any relapsing disease. We recommend that surgical treatment for laryngeal ACC be as conservative as possible.
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- 2021
9. Sclerosis of the arytenoid cartilage and glottic carcinoma: A clinical‐pathological study
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Federica Sovran, Piernicola Machin, Marco Lucioni, Andy Bertolin, Marco Lionello, Fabio Canal, and Giuseppe Rizzotto
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Male ,medicine.medical_specialty ,Cord ,Contrast Media ,Laryngectomy ,Sensitivity and Specificity ,03 medical and health sciences ,0302 clinical medicine ,Predictive Value of Tests ,otorhinolaryngologic diseases ,medicine ,Humans ,In patient ,030223 otorhinolaryngology ,Laryngeal Neoplasms ,Pathological ,Retrospective Studies ,Sclerosis ,Laryngoscopy ,medicine.diagnostic_test ,business.industry ,Cartilage ,Arytenoid cartilage ,Middle Aged ,respiratory system ,Endoscopy ,medicine.anatomical_structure ,Otorhinolaryngology ,Glottic cancer ,030220 oncology & carcinogenesis ,Radiological weapon ,Carcinoma, Squamous Cell ,Female ,Radiology ,Tomography, X-Ray Computed ,business ,Arytenoid Cartilage - Abstract
BACKGROUND Given the relevance of any tumor invasion of the arytenoid cartilage or crico-arytenoid unit to the planning open partial horizontal laryngectomy (OPHL) for laryngeal squamous cell carcinoma (LSCC), it is important to have a reliable radiological test to assess impairments of these structures. METHODS We retrospectively compared the endoscopic, radiological, and pathological findings in patients with glottic LSCC who underwent OPHL. RESULTS The endoscopic finding of a reduced (impaired or absent) vocal cord motility proved more sensitive, with better positive and negative predictive values, but less specific than the radiological finding of complete arytenoid sclerosis in detecting histologically assessable infiltration of the arytenoid cartilage. CONCLUSIONS Endoscopy retains a key role in the preoperative workup for glottic LSCC. CT evidence of complete sclerosis of the arytenoid cartilage is related to a dangerous contiguity of the tumor to the cartilage.
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- 2018
10. Colorectal polypoid lesions and expression of vascular endothelial growth factor in a consecutive series of endoscopic and surgical patients
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Anna Pozza, Luisa Toffolatti, Cesare Ruffolo, Marta Campo Dell'Orto, Carlo Castoro, Angelo Paolo Dei Tos, Francesco Ferrara, Marco Massani, Nicolò Bassi, Giulia Pagura, Andromachi Kotsafti, Marco Scarpa, and Fabio Canal
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Adult ,Male ,Vascular Endothelial Growth Factor A ,Cancer Research ,Pathology ,medicine.medical_specialty ,Colorectal cancer ,Carcinogenesis ,Messenger ,medicine.disease_cause ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,80 and over ,medicine ,Humans ,Neoplasm Invasiveness ,RNA, Messenger ,RC254-282 ,Aged ,Aged, 80 and over ,vascular endothelial growth factor ,business.industry ,Incidence (epidemiology) ,Case-control study ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,General Medicine ,colorectal malignant polyps ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,chemistry ,030220 oncology & carcinogenesis ,Case-Control Studies ,Colorectal Neoplasms ,Female ,RNA ,030211 gastroenterology & hepatology ,business ,Surgical patients - Abstract
Colorectal cancer incidence in patients undergoing screening protocols is decreasing because of the higher rate of discovered preneoplastic colonic lesions; however, adenomatous polyps may not always be removable endoscopically and surgery may still be necessary. The aim of this study was to assess the vascular endothelial growth factor expression in the different steps of colorectal carcinogenesis to explore its potential role as a marker of malignancy in polypoid lesions. A total of 92 subjects with colonic adenoma or cancer who underwent screening colonoscopy or surgery were prospectively enrolled. Real-time reverse transcription polymerase chain reaction for VEGF-A messenger RNA expression and immunohistochemistry for VEGF-A were performed. Immunoassays for VEGF-A, VEGF-C, VEGFR-1, VEGFR-2, and VEGFR-3 were also performed. Non-parametric statistics, receiver operating characteristic curve analysis, and logistic multiple regression analysis were used. VEGF-A messenger RNA expression was higher in patients with high-grade dysplasia or colorectal cancer than in those with low-grade dysplasia adenomas (p = 0.01). At immunohistochemistry, VEGF-A expression was significantly higher in colorectal cancer patients compared to dysplastic adenomas (p
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- 2017
11. Cytogenetic evidence of metastatic myxoid liposarcoma and therapy-related myelodysplastic syndrome in a bone marrow biopsy
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Filippo Gherlinzoni, Sabrina Rossi, Michele Gottardi, Lucia Zanatta, Stefano Licci, Licia Laurino, Fabio Canal, and Angelo Paolo Dei Tos
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Adult ,Pathology ,medicine.medical_specialty ,Bone Marrow Cells ,Soft Tissue Neoplasms ,Bone metastasis ,cytogenetics ,FISH ,Myxoid liposarcoma ,RAEB ,Therapy-related myelodysplastic syndrome ,2734 ,Liposarcoma ,Pathology and Forensic Medicine ,Antineoplastic Combined Chemotherapy Protocols ,Biopsy ,medicine ,Humans ,medicine.diagnostic_test ,business.industry ,Chromosomes, Human, Pair 11 ,Myelodysplastic syndromes ,Anemia, Refractory ,Neoplasms, Second Primary ,medicine.disease ,Combined Modality Therapy ,Liposarcoma, Myxoid ,Leukemia, Myeloid, Acute ,medicine.anatomical_structure ,Thigh ,Myelodysplastic Syndromes ,Female ,Bone marrow ,business ,Refractory anemia with excess of blasts ,Fluorescence in situ hybridization - Abstract
Myxoid liposarcoma exhibits a peculiar clinical behavior, with a tendency to spread to serosal membranes, distant soft tissues, and bones, even in the absence of lung metastases. Therapy-related hematological neoplasms are well-known side effects of cytotoxic chemotherapy. We describe an exceptional case of metastatic myxoid liposarcoma of the spine associated with therapy-related refractory anemia with excess of blasts in a 37-year-old woman who underwent multi-agent chemotherapy for a myxoid liposarcoma of the left thigh. Microscopic examination of the bone marrow biopsy revealed dysplastic features, with abnormal localization of immature precursors and micromegakaryocytes, and islands of undifferentiated oval small/medium-size cells, suggestive of acute myeloid leukemia arising in the setting of a myelodysplastic syndrome. Immunohistochemistry was not discriminant. Cytogenetic analyses of bone marrow aspirate disclosed the presence of 2 different rearrangements, subsequently confirmed by fluorescent in situ hybridization and was crucial in making the correct diagnosis.
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- 2009
12. Mismatch repair gene defects in sporadic colorectal cancer enhance immune surveillance
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Melania Scarpa, Marco Scarpa, Nicolò Bassi, Carlo Castoro, Silvia Basato, Angelo Paolo Dei Tos, Alain Fiorot, Lucia Dallagnese, Cesare Ruffolo, Ignazio Castagliuolo, Anna Pozza, Fabio Canal, Francesca Erroi, and Andrea Porzionato
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Oncology ,Male ,medicine.medical_specialty ,Pathology ,congenital, hereditary, and neonatal diseases and abnormalities ,Colorectal cancer ,chemical and pharmacologic phenomena ,Kaplan-Meier Estimate ,MLH1 ,DNA Mismatch Repair ,Polymerase Chain Reaction ,Mismatch repair ,Lymphocytes, Tumor-Infiltrating ,CD80 ,Surgical oncology ,Internal medicine ,medicine ,Humans ,neoplasms ,Survival analysis ,Aged ,Retrospective Studies ,Aged, 80 and over ,Tissue microarray ,Immune surveillance ,business.industry ,Middle Aged ,medicine.disease ,Flow Cytometry ,Molecular medicine ,Immunohistochemistry ,digestive system diseases ,MSH6 ,MSH2 ,Tissue Array Analysis ,B7-1 Antigen ,Female ,Microsatellite Instability ,business ,Colorectal Neoplasms ,HT29 Cells ,Research Paper - Abstract
// Marco Scarpa 1,* , Cesare Ruffolo 2,* , Fabio Canal 3 , Melania Scarpa 1 , Silvia Basato 4 , Francesca Erroi 4 , Alain Fiorot 2 , Lucia Dall’Agnese 4 , Anna Pozza 4 , Andrea Porzionato 5 , Ignazio Castagliuolo 5 , Angelo P. Dei Tos 3 , Nicolo Bassi 2 and Carlo Castoro 1 1 Surgical Oncology Unit, Veneto Institute of Oncology IOV-IRCCS, Padova, Italy 2 General Surgery Unit (IV), “Ca’ Foncello” Hospital, Treviso, Italy 3 Pathology Unit, “Ca’ Foncello” Hospital, Treviso, Italy 4 Department of Surgical, Oncological and Gastroenterological Sciences, University of Padova, Padova, Italy 5 Department of Molecular Medicine, University of Padova, Padova, Italy * These authors have contributed equally to this work Correspondence to: Marco Scarpa, email: // Keywords : mismatch repair, colorectal cancer, immune surveillance, CD80 Received : June 03, 2015 Accepted : October 07, 2015 Published : October 19, 2015 Abstract Background: There is evidence that colorectal cancers (CRC) with DNA mismatch repair deficiency (MMR-D) are associated with a better prognosis than the generality of large bowel malignancies. Since an active immune surveillance process has been demonstrated to influence CRC outcome, we investigated whether MMR-D can enhance the immune response in CRC. Patients and Methods: A group of 113 consecutive patients operated for CRC (42 stage I or II and 71 with stage III or IV) was retrospectively analyzed. The expression of MMR genes (MSH2, MLH1, MSH6 and PSM2) and co-stimulatory molecule CD80 was assessed by tissue microarray immunohistochemistry. In addition, tumor infiltrating mononuclear cells (TIMC) and T cell subpopulations (CD4, CD8, T-bet and FoxP-3) were quantified. The effect of specific siRNA (siMSH2, siMLH1, siMSH6 and siPSM2) transfection in HT29 on CD80 expression was quantified by flow cytometry. Non parametric statistics and survival analysis were used. Results: Patients with MMR-D showed a higher T-bet/CD4 ratio ( p = 0.02), a higher rate of CD80 expression and CD8 lymphocyte infiltration compared to those with no MMR-D. Moreover, in the MMR-D group, the Treg marker FoxP-3 was not expressed ( p = 0.05). MMR-D patients with stage I or II and T-bet expression had a significant better survival ( p = 0.009). Silencing of MSH2, MLH1 and MSH6, but not PSM2, significantly increased the rate of CD80+ HT29 cells ( p = 0.007, p = 0.023 and p = 0.015, respectively). Conclusions: CRC with MMR-D showed a higher CD80 expression, and CD8+ and Th1 T-cell infiltration. In vitro silencing of MSH2, MLH1 and MSH6 significantly increased CD80+ cell rate. These results suggest an enhanced immune surveillance mechanism in presence of MMR-D.
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- 2015
13. Rabbit Monoclonal Antibodies
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Licia Laurino, Angelo Paolo Dei Tos, Enrico Orvieto, Fabio Canal, Sabrina Rossi, Serena Chinellato, Fabio Facchetti, and Alberto Furlanetto
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CD3 Complex ,medicine.drug_class ,Synaptophysin ,CD5 Antigens ,Monoclonal antibody ,Sensitivity and Specificity ,Progesterone receptor ,Mice ,chemistry.chemical_compound ,Antigen ,Neoplasms ,Biomarkers, Tumor ,medicine ,Estrogen receptor ,Animals ,Cyclin D1 ,Immunohistochemistry ,monoclonal antibodies ,Antiserum ,biology ,Receptors, IgE ,Rabbit monoclonal antibody ,Antibodies, Monoclonal ,General Medicine ,Standardization ,2734 ,Molecular biology ,Ki-67 Antigen ,Receptors, Estrogen ,Antigen retrieval ,chemistry ,biology.protein ,Rabbits ,CD5 ,Antibody ,Receptors, Progesterone - Abstract
Rabbit monoclonal antibodies (RabMAbs) represent a novel category of immunoreagents that may combine the best properties of both mouse monoclonal antibodies (MMAs) and of rabbit antisera. In the attempt to verify the performance of this new class of antibodies on paraffin-embedded tissue, RabMAbs against estrogen receptor, progesterone receptor, Ki-67, cyclin D1, CD3, CD5, CD23, and synaptophysin were tested on several tumor types as well as normal tissues. The results were compared with those obtained with classic MMAs against the same antigens. RabMAbs appear to offer increased sensitivity with no apparent loss of specificity. On routine use they permit higher working dilutions (5 to 10 times on average), allowing significant improvement in terms of laboratory efficiency. The robustness of RabMAbs is further proved by the fact that in some instances optimal staining can be obtained even without antigen retrieval. In consideration of the high performance observed, routine use of RabMAbs may contribute significantly to standardize diagnostic immunohistochemical procedures.
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- 2005
14. Tu1863 TLR4 and MyD88 and Mismatch Repair Genes in Colorectal Cancer
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Alain Fiorot, Francesca Erroi, Angelo Paolo Dei Tos, Ignazio Castagliuolo, Anna Pozza, Marco Scarpa, Silvia Basato, Andromachi Kotsafti, Cesare Ruffolo, Carlo Castoro, Paola Brun, Melania Scarpa, and Fabio Canal
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Hepatology ,business.industry ,Colorectal cancer ,Gastroenterology ,TLR4 ,Cancer research ,Medicine ,DNA mismatch repair ,business ,medicine.disease - Published
- 2016
15. Prognostic Significance of Circulating and Endothelial Progenitor Cell Markers in Type 2 Diabetic Foot
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Filippo Gherlinzoni, Angelo Paolo Dei Tos, Agostino Paccagnella, Anna Furlan, Laura del Pup, Fabio Canal, Elena Seganfreddo, Monia Niero, and Maria Sambataro
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CD31 ,Pathology ,medicine.medical_specialty ,Article Subject ,business.industry ,CD34 ,Critical limb ischemia ,Calcitonin gene-related peptide ,medicine.disease ,Diabetic foot ,Endothelial progenitor cell ,Peripheral neuropathy ,RC666-701 ,Precursor cell ,Clinical Study ,cardiovascular system ,Diseases of the circulatory (Cardiovascular) system ,Medicine ,medicine.symptom ,business ,Cardiology and Cardiovascular Medicine - Abstract
Objective. We studied circulating precursor cells (CPC) in type 2 diabetes mellitus (T2DM) with neuropathic foot lesions with or without critical limb ischemia and relationships between endothelial precursor cells (EPC) and peripheral neuropathy.Methods and Subjects. We measured peripheral blood CD34, CD133, and CD45 markers for CPC and KDR, CD31 markers for EPC by citofluorimetry and systemic neural nociceptor CGRP (calcitonin gene related protein) by ELISA in 8 healthy controls (C) and 62 T2DM patients: 14 with neuropathy (N), 20 with neuropathic foot lesions (N1), and 28 with neuroischemic recent revascularized (N2) foot lesions. Timing of lesions was: acute (until 6 weeks), healed, and not healed.Results. CD34+ and CD133+ were reduced in N, N1, and N2 versus C, and CD34+ were lower in N2 versus N1 (P=0.03). In N2 CD34+KDR+ remain elevated in healed versus chronic lesions and, in N1 CD133+31+ were elevated in acute lesions. CGRP was reduced in N2 and N1 versus C (P<0.04versus C26±2 pg/mL). CD34+KDR+ correlated in N2 with oximetry and negatively in N1 with CGRP.Conclusions. CD34+ CPC are reduced in diabetes with advanced complications and diabetic foot. CD34+KDR+ and CD31+133+ EPC differentiation could have a prognostic and therapeutic significance in the healing process of neuropathic and neuroischemic lesions.
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- 2014
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16. MGMT promoter methylation and correlation with protein expression in primary central nervous system lymphoma
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S. Cavallin, Fabio Canal, Piero Maria Stefani, Elisa Scquizzato, Luisa Toffolatti, Filippo Gherlinzoni, A. P. Dei Tos, and Marco Scarpa
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Lymphoma ,Alkylating agents ,Immunohistochemistry ,MGMT ,Primary central nervous system lymphoma ,Promoter methylation ,2734 ,Cell Biology ,Molecular Biology ,Medicine (all) ,Biology ,medicine.disease_cause ,Pathology and Forensic Medicine ,Central Nervous System Neoplasms ,O(6)-Methylguanine-DNA Methyltransferase ,Glioma ,medicine ,Biomarkers, Tumor ,Temozolomide ,Gene silencing ,Humans ,Promoter Regions, Genetic ,neoplasms ,Aged ,General Medicine ,Methylation ,DNA Methylation ,Middle Aged ,medicine.disease ,Dacarbazine ,DNA methylation ,Cancer research ,Female ,Carcinogenesis ,medicine.drug - Abstract
The O (6)-methylguanine-DNA-methyltransferase (MGMT) gene encodes for a DNA repairing enzyme of which silencing by promoter methylation is involved in brain tumorigenesis. MGMT promoter methylation represents a favorable prognostic factor and has been associated with a better response to alkylating agents in glioma and systemic lymphoma. Primary central nervous system lymphoma (PCNSL) is a rare and aggressive extranodal malignant lymphoma. The current standard of care, based on high-dose methotrexate chemotherapy, has improved prognosis but outcome remains poor for a majority of patients. Therapeutic progress in this field is conditioned by limited biological and molecular knowledge about the disease. Temozolomide has recently emerged as an alternative option for PCNSL treatment. We aimed to analyze the MGMT gene methylation status in a series of 24 PCNSLs, to investigate the relationship between methylation status of the gene and immunohistochemical expression of MGMT protein and to evaluate the possible prognostic significance of these biomarkers. Our results confirm that methylation of the MGMT gene and loss of MGMT protein are frequent events in these lymphomas (54 % of our cases) and suggest that they are gender and age related. MGMT methylation showed high correlation with loss of protein expression (concordance correlation coefficient = -0.49; Fisher exact test: p 0.01), different from what has been observed in other brain tumors. In the subgroup of ten patients who received high dose chemotherapy, the presence of methylated MGMT promoter (n = 4), seems to be associated with a prolonged overall survival (60 months in three of four patients). The prognostic significance of these molecular markers in PCNSL needs to be further studied in groups of patients treated in a homogeneous way.
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- 2013
17. Complete remission induced by thalidomide in a case of angioimmunoblastic T-cell lymphoma refractory to autologous stem cell transplantation
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Angelo Paolo Dei Tos, Piero Maria Stefani, Elisabetta Calistri, Cristina Danesin, Ugo Salvadori, Fabio Canal, Michele Gottardi, and Filippo Gherlinzoni
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Cancer Research ,Angioimmunoblastic T-cell lymphoma ,business.industry ,Conventional treatment ,Complete remission ,Clinical course ,Hematology ,medicine.disease ,Lymphoma ,Thalidomide ,Autologous stem-cell transplantation ,Oncology ,Refractory ,immune system diseases ,hemic and lymphatic diseases ,Cancer research ,medicine ,business ,medicine.drug - Abstract
Angioimmunoblastic T-cell lymphoma (AITL) is a peripheral T-cell lymphoma characterised by a very aggressive clinical course. Despite conventional treatment, usually intensive adriamycin-containing...
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- 2008
18. An unusual mediastinal outline
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Fabio Canal, Alessandra Bianchi, Alberto Banzato, Valentina Polo, and Roberta Polverosi
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Adult ,Male ,medicine.medical_specialty ,Thymoma ,medicine.medical_treatment ,Biopsy ,chemical and pharmacologic phenomena ,Asymptomatic ,Predictive Value of Tests ,hemic and lymphatic diseases ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,neoplasms ,Neoadjuvant therapy ,Asymptomatic Diseases ,Incidental Findings ,medicine.diagnostic_test ,business.industry ,General Medicine ,Thymus Neoplasms ,medicine.disease ,Thymectomy ,Primary Neoplasm ,Neoadjuvant Therapy ,surgical procedures, operative ,Echocardiography ,Predictive value of tests ,Radiology ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Tomography, X-Ray Computed - Abstract
Thymoma is the most common primary neoplasm of the anterior mediastinum. We describe the case of a 40-year-old man with asymptomatic thymoma involving the right atrium, and the diagnostic pathway.
- Published
- 2011
19. Pituitary metastasis of Merkel cell carcinoma
- Author
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Alberto Feletti, Pierluigi Longatti, Fabio Canal, Elisabetta Marton, Domenico Billeci, and Sabrina Rossi
- Subjects
Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Skin Neoplasms ,Merkel cell carcinoma ,Metastasis ,Hypophysis ,Brain ,Unknown primary site ,Pituitary adenoma ,Internal medicine ,Humans ,Medicine ,Pituitary Neoplasms ,Aged ,business.industry ,food and beverages ,Neoplasms, Second Primary ,medicine.disease ,Immunohistochemistry ,Carcinoma, Merkel Cell ,Neurology ,Pituitary metastasis ,Cancer research ,Neurology (clinical) ,business - Abstract
Merkel cell carcinoma (MCC) is a malignant neuroendocrine tumor of the skin that demonstrates a remarkable tendency to metastasize. However, only a few cases of MCC brain metastases have been reported in the literature. We here present a unique case of a pituitary metastasis of MCC in a 65-year-old patient with a history of pituitary adenoma. This case is particularly novel due to the fact that the primary site of the MCC is unknown.
- Published
- 2010
20. Aleukemic granulocytic sarcoma with associated T-cell lymphoblastic lymphoma in the same lymph node: Morphologic features and molecular signatures
- Author
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Angelo Paolo Dei Tos, Sabrina Rossi, Stefano Licci, Lucia Zanatta, Filippo Gherlinzoni, Marnì Fedrigo, Alessia Brenna, and Fabio Canal
- Subjects
Oncology ,medicine.medical_specialty ,Pathology ,Acute leukemia ,Cancer Research ,Hematology ,business.industry ,T cell ,Lymphoblastic lymphoma ,medicine.disease ,Undifferentiated Acute Leukemia ,medicine.anatomical_structure ,hemic and lymphatic diseases ,Internal medicine ,Medicine ,Sarcoma ,business ,Who classification ,Lymph node - Abstract
The concept of bilineal acute leukemia is defined in the WHO classification, where, along with the undifferentiated acute leukemia and the biphenotypic acute leukemia, it is included in the group o...
- Published
- 2008
21. Small round-cell neoplasms of soft tissues: An integrated diagnostic approach
- Author
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Angelo Paolo Dei Tos, Sabrina Rossi, Antonio G. Nascimento, and Fabio Canal
- Subjects
Round-cell sarcoma ,Pathology ,medicine.medical_specialty ,Alveolar rhabdomyosarcoma ,Chondrosarcoma ,Ewing's sarcoma ,Immunohistochemistry ,Molecular genetics ,PNET ,Synovial sarcoma ,2734 ,Soft tissue ,Context (language use) ,Biology ,medicine.disease ,Mesenchymal chondrosarcoma ,Pathology and Forensic Medicine ,medicine ,Sarcoma - Abstract
Summary The family of small round-cell tumours (SRCTs) represents a heterogeneous group of malignancies featuring a primitive, undifferentiated round-cell morphology. SRCTs mostly occur in children, adolescents and young adults, and tend to involve the skeletal system or soft tissue. They constitute approximately 20% of solid tumours in children and, because of their significant morphological overlap, have become a paradigm for an integrated approach to diagnosis. The combination of both immunophenotypic and genetic analysis with classic morphology has proved useful not only on diagnostic grounds, but also in the context of prognostication. This review will focus on SRCTs primarily involving soft tissues and includes the Ewing's family of tumours, also known as Ewing's sarcoma/primitive neuroectodermal tumour, alveolar rhabdomyosarcoma, desmoplastic SRCT, poorly differentiated round-cell synovial sarcoma and mesenchymal chondrosarcoma.
- Published
- 2007
22. [Environmental pollution from dioxins and soft tissue sarcomas in the population of Venice and Mestre: an example of the use of current electronic information sources]
- Author
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Roberta, Tessari, Cristina, Canova, Fabio, Canal, Sergio, Lafisca, Andrea, Inio, Bruno, Murer, Vincenzo, Stracca, Mauro, Tollot, and Lorenzo, Simonato
- Subjects
Male ,Incidence ,Lymphoma, Non-Hodgkin ,Sarcoma ,Environmental Exposure ,Incineration ,Dioxins ,Hodgkin Disease ,Sex Factors ,Databases as Topic ,Italy ,Risk Factors ,Humans ,Environmental Pollutants ,Female - Abstract
Estimating the potential carcinogenic risk associated with exposure to dioxins generated by industrial emissions and urban incinerators.We conducted a geographical investigation on the relationship between Soft Tissue Sarcomas (STS) and other tumours, and estimated levels of exposures to dioxins. Estimates ofthe population's exposure were derived from a historical analysis of emissions of dioxins and other pollutants, conducted by the Province of Venice in all the Venetian area in 1980-1990. Cancer cases were selected from two electronic pathology databases of Venice and Mestre, computerized since 1987. All STS diagnoses were revised in order to improve the quality of the cases in the study and cases of Kaposi sarcoma were excluded from the analysis. The 198 remaining STS--Hodgkin's lymphoma, non-Hodgkin' lymphoma and subjects with at least one cancer--were linked to the registry of residents and mapped according to the pollutant level estimates using GIS techniques. The same procedure was applied to cases of non-Hodgkin's lymphoma (n=822), Hodgkin's lymphoma (n=142) and to 25.568 cases of all cancer at other sites, with microscopic confirmation for comparison.Venetian AULSS 12.Risks for STS (measured as Standardized Incidence Ratios SIR) did not show any trend related to categories of dioxin pollution. Among women, a statistically significant increased SIR of 1.69 was estimated for the highest category of exposure. The corresponding SIR for Hodgkins lymphoma was 1.94 (I.C. 1.08-1.19). No major excesses were estimated for other cancer sites.Overall, no consistent association between cancer risk and estimated exposure to dioxins was detected in the population under study However, a statistically significant excess of STS among women in the highest exposure category allows for the possibility of a role of environmental exposure.
- Published
- 2006
23. Concomitant chronic lymphocytic leukemia and acute myeloid leukemia: Evidence of simultaneous expansion of two independent clones
- Author
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Angelo Paolo Dei Tos, Massimo Degan, Cristina Tecchio, Licia Laurino, Valter Gattei, Riccardo Bomben, Filippo Gherlinzoni, Fabio Canal, Maurizio Mordacchini, Lucia Zanatta, Antonella Zucchetto, and Michele Gottardi
- Subjects
Male ,medicine.medical_specialty ,Cancer Research ,Chronic lymphocytic leukemia ,CD33 ,Biology ,Immunophenotyping ,Neoplasms, Multiple Primary ,immune system diseases ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Humans ,neoplasms ,Acute myeloid leukemia ,IgVH gene mutational status ,Simultaneous diagnosis ,Hematology ,Oncology ,Aged ,Cell Proliferation ,Gene Rearrangement ,Myeloid leukemia ,Gene rearrangement ,medicine.disease ,Leukemia, Lymphocytic, Chronic, B-Cell ,Clone Cells ,Leukemia ,Leukemia, Myeloid ,Immunology ,Acute Disease ,Cancer research ,Neoplastic cell ,Immunoglobulin Heavy Chains - Abstract
The simultaneous appearance of chronic lymphocytic leukemia (CLL) and acute myeloid leukemia (AML) has been rarely reported, with AML occurring more frequently as a secondary event in patients receiving cytotoxic drugs for a primary lymphoproliferative disorder. We describe a case of simultaneous CLL and AML documented by morphological and cytometric analysis in a previously untreated patient. In particular, on the basis of morphological and immunological features, the patient was diagnosed as being affected by CD34 + /CD13 + /CD33 + /HLA-DR + /CD7 + FAB-M2 AML, along with a B-CLL characterized by neoplastic cells expressing a VH3-53/D3-22/JH4 Ig, bearing, on average, 3.9% IgVH mutations without evidence of antigen-driven selection. To establish whether the two neoplastic cell populations shared some common molecular signature, we performed IgH gene rearrangement studies on CD34 + /CD19- and CD34-/CD19 + immunomagnetically sorted cell populations: only genomic DNA from the CD19 + /CD34- cell fraction revealed the presence of the IgH gene rearrangement. These results provide evidence that the rare concomitant association of CLL and AML likely arises from simultaneous expansion of two independent clones.
- Published
- 2006
24. Inquinamento ambientale da diossine e Sarcomi dei Tessuti Molli nella popolazione di Venezia Mestre: un esempio di utilizzo di fonti informative elettroniche in indagini epidemiologiche su rischi ambientali
- Author
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Roberta, Tessari, Canova, Cristina, Fabio, Canal, Sergio, Lafisca, Andrea, Inio, Bruno, Murer, Vincenzo, Stracca, Mauro, Tollot, and Simonato, Lorenzo
- Published
- 2006
25. P53 and p21waf1 expression by immunohistochemistry in diffuse large B-cell lymphoma has a strong and independent impact on survival of patients with germinal center phenotype
- Author
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Claudia Parolini, Fabio Canal, Maurizio Lestani, Giovanni Pizzolo, Annalisa Andreoli, Achille Ambrosetti, Mauro Krampera, Marco Chilosi, and Carlo Visco
- Subjects
Acute aortic syndrome ,medicine.medical_specialty ,medicine.medical_treatment ,Immunology ,Germinal center ,Cell Biology ,Hematology ,Biology ,medicine.disease ,Biochemistry ,Phenotype ,Gastroenterology ,Lymphoma ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Cancer research ,Immunohistochemistry ,Rituximab ,Diffuse large B-cell lymphoma ,Neoadjuvant therapy ,medicine.drug - Abstract
Over-expression of P53-related protein detected by immunohistochemistry (IHC) and absence of p21waf1— expression, the main downstream target following P53 activation, are useful surrogate markers in identifying diffuse large B-cell lymphoma (DLBCL) patients with mutated P53-gene. Following recent studies on gene expression, tissue micro-array technology has been shown to represent a reliable technique in discriminating DLBCL with germinal center B-cell like (GC), or post- germinal center (non-GC) origin. We analyzed 80 consecutive patients with de-novo DLBCL, homogeneously treated with cure-intent (CHOP-like regimens +/− Rituximab) in a single Institution, for expression of p53/p21waf1—, with regard to the expression of GC (CD10+, bcl-6+/mum-1−/CD138−), and post-germinal center (CD10−, mum-1+, CD138+) IHC markers. Cases with 50% or more p53-positive neoplastic cells were defined as p53+++. All patients were provided with complete clinical information. Median age of our patients was 50 years, AAS was III or IV in half of patients, IPI was high or int/high in 36%. Median follow-up for survivors was 77 months. A p53+++/p21waf1— phenotype (corresponding to loss of function of the P53-gene) was detected in 19 of 80 patients (23%), while a GC phenotype characterized 42 patients (52%). Patients with P53+++/p21waf1— phenotype were more frequently resistant to induction therapies (p Our results strongly point to the usefulness of a combined molecular and clinical approach for prognostic considerations in DLBCL, and should be validated in larger series.
- Published
- 2005
26. Su1923 Immune Microenvironment in Colonic Carciongenesis: Sporadic Mismatch Repair Genes Defects Are Associated to Hicd80+ Lamina Propria Monuclear Cells Infiltration
- Author
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Angelo Paolo Dei Tos, Ignazio Castagliuolo, Silvia Basato, Melania Scarpa, Cesare Ruffolo, Andrea Porzionato, Anna Pozza, Nicolò Bassi, Fabio Canal, Carlo Castoro, Francesca Erroi, Alain Fiorot, and Marco Scarpa
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,education.field_of_study ,Hepatology ,business.industry ,Colorectal cancer ,Population ,Gastroenterology ,nutritional and metabolic diseases ,Microsatellite instability ,MLH1 ,medicine.disease ,complex mixtures ,digestive system diseases ,MSH6 ,MSH2 ,DNA methylation ,Cancer research ,Medicine ,DNA mismatch repair ,education ,business - Abstract
BACKGROUND Genomic defects in DNA mismatch repair (MMR) genes (MSH2, MLH1, PSM2 or MSH6) characterize the hereditary non polyposis colon cancer (HNPCC). However most colorectal cancers (CRC) with high-frequency microsatellite instability are sporadic, wherein the MMR defect develops because of inactivation of the MLH1 gene by DNA methylation. Multiple retrospective studies, including a population-based study and a metaanalysis, have demonstrated that patients with MMR-deficient colon cancers have a more favourable stage-adjusted prognosis compared with patients whose tumors have intact MMR. Since the immune environment of CRC has been demonstrated to influence its prognosis, we aimed to investigate the interplay between MMR genes and the immune environment in CRC PATIENTS AND METHODS A group of 98 consecutive patients operated on for colorectal cancer was retrospectively analysed. Familial and medical history were retrieved to assess the presence of Bethesda criteria for HNPCC diagnosis. Immunohistochemistry for CD80, TLR4, MyD88, MSH2, MLH1, MSH6 and PSM2 was performed on tissue sections. Moreover, lamina propria mononuclear cells (LPMC), polymorphonuclear cells and eosoinophil tumour infiltration was quantified. Patients were stratified in three groups: no MMR genes defect, MMR gene defects alone and MMR genes defects and at least one positive Bethesda criteria. Non parametric statistics was used. RESULTS In the three groups no difference was observed in term of polymorphonuclear cells and eosinophil infiltration and in term of TLR4 and MyD88 expression. On the contrary, LPMC infiltration was significantly higher in patients who had a MMR gene defect alone compared to patients who had no MMR genes defect and to those with MMR gene defect and positive Bethesda criteria (p= 0.014). Similarly, a significantly higher frequency of patients with high CD80 expression was observed in patients who had a MMR genes defect alone compared to patients who had no MMR gene defect and to those with MMR genes defect and positive Bethesda criteria (p=0.048). CONCLUSION In patients with MMR defects and no Bethesda criteria for HNPCC antigen presenting cells function seems enhanced as shown by higher frequency of hiCD80+ patients and higher LPMC infiltration. This immune activation may play a role in the prognosis of these patients.
- Published
- 2014
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