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Dung beetles are a functionally important group of species, and the effects of large wild herbivores on these species are not well understood. Management of wild herbivores could, therefore, have considerable consequences for dung beetle assemblages and the ecosystem services they provide. Here, we analysed how species richness, biomass, abundance, and composition of dung beetle assemblages were affected by different dung availability as a consequence of different red deer (Cervus elaphus) densities under field conditions. This was done in spring and autumn over four independent study regions in Central Europe where red deer are the most important providers of dung in forests. Our results show that forest sites with high dung availability support higher species richness of dung beetles than sites with low availability. This effect is independent of season and could be explained by the different colonisation patterns of beetle species which vary in their competitiveness. As aggregated dung resources increase opportunities for low-competitive species, those species establish small populations and do not necessarily increase the abundance of the whole assemblage. Our results show that the current policies to limit deer populations also limit higher levels of species diversity of this functionally important arthropod taxon. We highlight the importance of hunting-free areas for the conservation of insect diversity associated with large herbivores. In strictly protected areas with low herbivore densities we suggest to reduce control of ungulate numbers to provide sufficient resources for maintaining dung beetle diversity.

Submerged cultures of the basidiomycota Cystostereum murrayi emit an intensive coconut-like, sweetish, and buttery smell. For identification of the key aroma compounds, an aroma dilution analysis using dynamic headspace was performed by adjusting the split ratio of the GC inlet system. Flavor dilution (FD) factors varied from 22 up to ≥218, whereby the largest class of compounds represented terpenoids, including two rare stereoisomers of 3,6-dimethyl-2,3,3a,4,5,7a-hexahydrobenzofuran (dill ether, ee ≥ 99.9). By means of nuclear magnetic resonance spectroscopy, the substances with the highest FD factors (29, 212, and 218) were identified as diastereomers of 3,6-dimethyl-3a,4,5,6,7,7a-hexayhydro-3H-1-benzofuran-2-one (dihydromenthofurolactone) and as its corresponding C3-unsaturated lactone. The latter two compounds have not been described for Cystostereum murrayi or for any other basidiomycota previously. Supplementation studies using 2-13C-d-glucose indicated that these lactones as well as the two stereoisomers of dill ether and other terpenoids were formed de novo by the fungus.

Root-colonizing fungi form species-rich assemblages with key functions in principal ecosystem processes, making them prospectively important players in conservation and applied ecology. Harnessing the processes and services they drive requires a better understanding of their patterns of diversity and community structure, and how these link to function. Here, we search for possible adaptations to contrasting environmental and host conditions, indicative of participation in habitat-specific processes. We surveyed heathland and grassland habitats across a latitudinal gradient in Western Europe, using a spatially explicit design to assess community variation at scales from centimeters, to thousands of kilometers. Root-associated fungi assemble into strongly site-specific communities irrespective of habitat type, shaped by environmental factors and spatial distance operating at different scales, but also by a high level of endemism, likely to be determined by local stochastic processes such as drift and dispersal limitation at short distances. Despite the high site specificity in communities, they are dominated everywhere by a core set of lineages with little preferences toward habitat conditions or host phylogeny. Our results suggest a convergent evolution across phylogenetically distant lineages toward the root-colonizing habit, and a functional redundancy in strategies for habitat colonization and host interaction. Further efforts are needed to integrate functional trait composition in future community ecology studies of root-colonizing fungi.

Submerged cultures of the basidiomycota

Protected areas worldwide are important to maintaining biodiversity and providing recreational opportunities to society. However, many protected areas are affected by unprecedented, large and severe natural disturbances, like bark beetle outbreaks. Due to the contrasting responses of different taxonomic groups to disturbance events and largely negative human perceptions of disturbed landscapes, there are conflicting opinions about the appropriate way of managing affected stands. Aligning these different objectives and understanding the responses of biodiversity and visitors' perceptions to different disturbance severities is a prerequisite for disturbance management in protected areas. We conducted multi-taxon biodiversity surveys – including meta-barcoding hyperdiverse groups such as insects and fungi – and analysed the restorativeness (i.e. the landscape's ability to renew personal cognitive capacities for forest visitors) using visitor surveys in five national parks throughout Europe. Response curves of biodiversity and restorativeness were analysed along a continuous gradient of bark beetle infestation severities in Norway spruce forests on the same study plots. Arthropod biomass and the diversity of primary producers and pollinators increased linearly with increasing disturbance severity, while overall multi-diversity (an index of the average scaled species richness per taxonomic group) did not change. Restorativeness decreased linearly with increasing disturbance severity; however, even heavily disturbed forests still had high restorativeness. In spite of the ongoing debates about disturbance management, the high biodiversity and restorativeness that accompany disturbance suggest that major goals of protected areas are not threatened by bark beetle disturbances.

Although taxonomic knowledge on Tricholoma (Agaricales, Basidiomycota) is fairly comprehensive in northwest Europe, knowledge of the global diversity and distribution of Tricholoma spp. is still sparse. In this study, the diversity and distribution of some Tricholoma spp. are analyzed by morphological and molecular methods based on 70 collections from Yunnan, China, 45 from central Europe, 32 from Colorado, USA, 9 from Japan, and 3 from Ukraine. A Holarctic distribution is suggested for several species, based on collections and nuc rDNA internal transcribed spacer ITS1-5.8S-ITS2 (ITS) sequences. Six species new to science are formally described from Yunnan: five in existing sections, Tricholoma forteflavescens, T. olivaceoluteolum, T. melleum, T. olivaceum, and T. sinoportentosum, and one, T. muscarioides, in the newly described section Muscaria alongside several previously described species. Additional putatively new species cannot be formally described because they lack sufficient material. Tricholoma foliicola is recognized as a species of the genus Gerhardtia.

The fungal genus Strobilurus belongs to Physalacriaceae and contains approximately 11 species worldwide. Species of this genus grow and reproduce on cones of various conifers, seed pods or fruits of Magnolia and Liquidambar, and branches and wood of conifers. Previous studies focused mainly on samples from Europe and North America. And no genus-specific phylogenetic analysis has been carried out to date. The monophyly, degree of species diversity and substrate specificity, and overall distribution patterns are addressed here using morphological and molecular evidence. The authors collected samples of Strobilurus from much of its known distribution ranges and carried out morphological observations and multilocus phylogenetic analyses using five molecular markers. The results show that Strobilurus is a monophyletic group but may exclude one species, S. ohshimae. A total of 13 species was identified, with two, S. orientalis and S. pachycystidiatus, described as new from China. Several species were shown to be specific to certain substrates, whereas a few less so. Biogeographic analyses indicated that historical exchanges of species between East Asia, Europe, and North America, later vicariance events, and substrate specificity have contributed jointly to diversification of Strobilurus.

The aroma compounds of fruiting bodies of C. odorifer were extracted by means of liquid/liquid extraction and purified by solvent assisted flavour evaporation (SAFE). The odour active compounds were identified by gas chromatography-mass spectrometry and olfactometry on two columns of different polarity and described by sensory detection. The volatilom of C. odorifer is reported here for the first time. Additionally, an aroma extract dilution analysis (AEDA) was performed to identify the main flavour compounds. Finally, the volatiles were quantified by GC flame ionization detection (GC-FID). The main odour compound of C. odorifer was methyl p-anisate with an FD factor of 8,192 and a concentration of 120 mg kg-1. Methyl p-anisate was also the compound responsible for the characteristic anise-like odour of C. odorifer. Copyright © 2015 John Wiley & Sons, Ltd.

Purple Laccaria are ectomycorrhizal basidiomycetes associated with temperate forests all over the Northern Hemisphere in at least two taxa: Laccaria amethysteo-occidentalis in North America, and L. amethystina complex in Eurasia, as shown by Vincenot et al. (2012). Here, we combine a further study of the genetic structure of L. amethystina populations from Europe to southwestern China and Japan, using neutral Single Sequence Repeat (SSR; microsatellite) markers; and a systematic description of two novel Asian species, namely Laccaria moshuijun and Laccaria japonica, based on ecological, morphological, and molecular criteria (rDNA sequences). Population genetics provides evidence of the ancient isolation of three regional groups, with strong signal for speciation, and suggests a centre of origin of modern populations closest to present-day Chinese populations. Phylogenetic analyses confirm speciation at the molecular level, reflected in morphological features: L. moshuijun samples (from Yunnan, China) display strongly variable cheilocystidia, while L. japonica samples (from Japan) present distinctive globose to subglobose spores and clavate cheilocystidia. This study of a species complex primarily described with an extremely wide ecological and geographical range sheds new light on the biodiversity and biogeography of ectomycorrhizal fungi.

Here we present the first checklist of the UNESCO World Natural Heritage Kellerwald-Edersee National Park in the federal state of Hesse in Germany. After 10 years of investigation, 1107 species have been recorded. Thus far, efforts have led to the detection of 44 species of interest according to the criteria of the International Union for the Conservation of Nature and Natural Resources (IUCN), 10 species with nature value based on a European scale, and 16 nature-value species on a German scale. Sixty-four threatened species are listed on the German red list of fungi, and 147 species are listed on the regional red list of the federal state of Hesse. As part of the UNESCO World Natural Heritage Ancient Beech Forests of Germany and Primeval Beech Forests of the Carpathians, the Kellerwald-Edersee National Park represents the typical European natural beech forest on poor soil with old tree stands and relict primeval forest fragments. The presence of nature-value species in the beech (Fagus sylvatica)-dominant Kellerwald-Edersee and Hainich national parks shows the development of their forest ecosystems to near naturalness comparable to the primeval forests of the Poloniny National Park in Slovakia.

In this paper descriptions of three new Laccaria species from Southwest China (Yunnan) are reported. Macromorphological, micromorphological, and molecular data have been studied to describe the new species and delineate them within the genus Laccaria. The first species Laccaria fulvogrisea is characterized by a grey to brownish coloured fruiting body and large echinulate spores. Laccaria aurantia is characterized by the deeply orange colour and globose balloon-like spores with a fine echinulate ornament. The third species Laccaria yunnanensis has large barrel-shaped pleurocystidia and a brownish to flesh-coloured fruiting body. All three species have 4-spored basidia and were found near or within Quercus and Lithocarpus mixed broad leaved forests at an altitude of above 2,000 m.