310 results on '"Food and Nutrition Department"'
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2. The harmonized INFOGEST in vitro digestion method: From knowledge to action
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Neil M. Rigby, Lonneke M. JanssenDuijghuijsen, Didier Dupont, Irene Comi, Beatriz Miralles, Anne Meynier, Helena Stoffers, Paula Alvito, Anne Pihlanto, Laurie Eve Rioux, Sibel Karakaya, Brent S. Murray, Ana Tavares, María Jesús Lagarda, Steven Le Feunteun, Guadalupe Garcia-Llatas, Sebnem Simsek, Alfonso Clemente, Isidra Recio, Alan R. Mackie, Gianluca Picariello, Lotti Egger, Ellen Kathrine Ulleberg, Olivia Ménard, Reto Portmann, Reyes Barberá, André Brodkorb, Gerd E. Vegarud, Cristina Delgado-Andrade, Lucélia Tavares, Uri Lesmes, Sylvie L. Turgeon, Carla Martins, Guy Vergères, Simon Balance, Thomas Cattenoz, Ricardo Assunção, Cláudia N. Santos, Agroscope, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Department of Physiology and Biochemistry of Animal Nutrition, Estacion Experimental del Zaidin, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge I.P., National Institute of Health Doutor Ricardo Jorge, I.P, Nofima AS, Nutrition and Food Science Area, University of Valencia, Teagasc Food Research Centre [Fermoy, County Cork, Ireland], Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Wageningen University and Research Centre, Food and Biobased Research, Ege University, Engineering Faculty Department of Food Engineering, Department of Biotechnology and Food Engineering, Technion, Israel Institute of Technology, Institute of Food Research, National Institute of Health Doutor Ricardo Jorge, I.P., Food and Nutrition Department, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Instituto de Investigaciòn en Ciencias de la Alimentaciòn (CIAL, CSIC-UAM), School of Food Science and Nutrition, University of Leeds, Natural Resource Institute Finland (LUKE) New Business Opportunities, Institute of Food Sciences, National Research Council of Italy (CNR), Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa (NOVA), iBET, Instituto de Biologia Experimental e Tecnológica, Apartado, Institute of Food Research, Norwich, STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Chercheur indépendant, Cost Infogest, COST Action FA1005 INFOGEST, MycoMix project, Fundação para a Ciência e Tecnologia, iNOVA4Health, PTDC/DTP-FTO/0417/2012, UID/Multi/04462/2013, IF/01097/2013, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Teagasc Food Research Centre [Fermoy, Ireland], AgroParisTech-Institut National de la Recherche Agronomique (INRA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Instituto de Biologia Experimental e Tecnológica (IBET), and Ege Üniversitesi
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harmonisation de méthode ,Harmonized IVD protocol ,Hydrolyzed protein ,partage de connaissance ,connaissance partagée ,Regulation of gastric function ,méthode de recherche ,digestion ,0404 agricultural biotechnology ,Pepsin ,digestion in vitro ,Inter-laboratory trial ,INFOGEST network ,Levensmiddelenchemie ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Medicine ,Knowledge to action ,Cost action ,Food research ,Food science ,Food, Health & Consumer Research ,2. Zero hunger ,collaboration scientifique ,biology ,Food Chemistry ,Mass spectrometry ,business.industry ,In vitro digestion ,collaboration européenne ,04 agricultural and veterinary sciences ,040401 food science ,scientific co-operation ,Composição dos Alimentos ,research method ,Health & Consumer Research ,Biochemistry ,Food ,biology.protein ,Dairy proteins ,Digestion ,business ,Peptides ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,méthode d'harmonisation ,Food Science - Abstract
4th International Conference on Food Digestion -- MAR, 2015 -- Naples, ITALY, WOS: 000384789000006, Within the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skim milk powder (SMP) as a model food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, the degree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas beta-lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase. The study also showed that a few critical steps were responsible for the remaining inter-laboratory variability. The largest deviations arose from the determination of pepsin activity. Therefore, this step was further clarified, harmonized, and implemented in a third inter-laboratory study. The present work gives an overview of all three inter-laboratory studies, showing that the IVD INFOGEST method has led to an increased consistency that enables a better comparability of in vitro digestion studies in the future. (C) 2015 The Authors. Published by Elsevier Ltd.
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- 2016
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3. Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
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Gijsje H. Koenderink, Manos Mavrakis, Aurélie Bertin, Carla Silva Martins, Francois Iv, Jeffrey den Haan, Gerard Castro-Linares, Delft University of Technology (TU Delft), Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), MOSAIC (MOSAIC), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Food and Nutrition Department, Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Centro de Estudos do Ambiante e do Mar (CESAM), Universidade de Aveiro, National School of Public Health, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Netherlands Organization for Scientific Research (NWO/OCW) through the 'BaSyC- Building a Synthetic Cell' Gravitation grant (024.003.019), ANR-17-CE13-0014,SEPTIMORF,Rôle des septines dans la morphogenèse des cellules animales(2017), ANR-13-JSV8-0002,SEPTIME,Assemblage et ultrastructure des septines, de la molécule à la cellule(2013), and ANR-20-CE11-0014,SeptScort,Interaction spatiale et fonctionnelle entre septines et Escrt pendant la cytocinèse: une approche multi-échelle(2020)
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Quality Control ,General Immunology and Microbiology ,General Chemical Engineering ,General Neuroscience ,fungi ,macromolecular substances ,[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,General Biochemistry, Genetics and Molecular Biology ,[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,GTP-Binding Proteins ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,Humans ,Protein Isoforms ,biological phenomena, cell phenomena, and immunity ,Cytoskeleton ,Septins - Abstract
International audience; Septins are a family of conserved eukaryotic GTP-binding proteins that can form cytoskeletal filaments and higher-order structures from hetero-oligomeric complexes. They interact with other cytoskeletal components and the cell membrane to participate in important cellular functions such as migration and cell division. Due to the complexity of septins' many interactions, the large number of septin genes (13 in humans), and the ability of septins to form hetero-oligomeric complexes with different subunit compositions, cell-free reconstitution is a vital strategy to understand the basics of septin biology. The present paper first describes a method to purify recombinant septins in their hetero-oligomeric form using a two-step affinity chromatography approach. Then, the process of quality control used to check for the purity and integrity of the septin complexes is detailed. This process combines native and denaturing gel electrophoresis, negative stain electron microscopy, and interferometric scattering microscopy. Finally, a description of the process to check for the polymerization ability of septin complexes using negative stain electron microscopy and fluorescent microscopy is given. This demonstrates that it is possible to produce high-quality human septin hexamers and octamers containing different isoforms of septin_9, as well as Drosophila septin hexamers.
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- 2022
4. Extending in vitro digestion models to specific human populations: Perspectives, practical tools and bio-relevant information
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Levi, Carmit Shani, Alvito, Paula, C, Andrés, Ana, Assunção, Ricardo, Barberá, Reyes, Blanquet Diot, Stéphanie, Bourlieu, Claire, Brodkorb, André, Cilla, Antonio, Deglaire, Amélie, Denis, Sylvain, Dupont, Didier, Heredia, Ana, Karakaya, Sibel, GIOSAFATTO, CONCETTA VALERIA LUCIA, MARINIELLO, LOREDANA, Martins, Carla, Ménard, Olivia, El, Sedef Nehir, Vegarud, Gerd Elizabeth, Ulleberg, Ellen, Lesmes, Uri, Department of Biotechnology and Food Engineering, Israel Institute of Technology, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge I.P., CESAM, Centre for Environmental and Marine Studies, University of Aveiro, Institute of Food Engineering for Development, Universitat Politecnica de Valencia (UPV), Faculty of Pharmacy, University of Valencia, Conception, Ingénierie et Développement de l'Aliment et du Médicament (CIDAM), Université d'Auvergne - Clermont-Ferrand I (UdA), Teagasc Food Research Centre [Fermoy, County Cork, Ireland], Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Food Engineering Department, Ege university, Department of Chemical Sciences, University of Naples Federico II, Norwegian University of Life Sciences (NMBU), Ege Üniversitesi, Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Universidade de Aveiro, Universitat Politècnica de València (UPV), Teagasc Food Research Centre [Fermoy, Ireland], Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Levi, Carmit Shani, Alvito, Paula, Andrés, Ana, Assunção, Ricardo, Barberá, Reye, Blanquet Diot, Stéphanie, Bourlieu, Claire, Brodkorb, André, Cilla, Antonio, Deglaire, Amélie, Denis, Sylvain, Dupont, Didier, Heredia, Ana, Karakaya, Sibel, Giosafatto, CONCETTA VALERIA LUCIA, Mariniello, Loredana, Martins, Carla, Ménard, Olivia, El, Sedef Nehir, Vegarud, Gerd Elizabeth, Ulleberg, Ellen, Lesmes, Uri, and University of Naples Federico II = Università degli studi di Napoli Federico II
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Digestão in vitro ,0301 basic medicine ,Engineering ,enzymic activity ,TECNOLOGIA DE ALIMENTOS ,Food digestion ,Population ,Avaliação de Risco ,personne âgée ,modèle in vitro ,03 medical and health sciences ,Elderly ,Digestion (alchemy) ,digestion in vitro ,Saúde Humana ,Gastric ,Food and Nutrition ,activité gastrointestinale ,In vitro Digestion ,Gastro-intestinal disorders ,education ,senior citizens ,Toxicoligia ,fibrose cystique ,2. Zero hunger ,education.field_of_study ,030109 nutrition & dietetics ,Gastric emptying ,Scope (project management) ,business.industry ,activité enzymatique ,In vitro digestion ,Infants ,Food Digestion ,Gastric ph ,Biotechnology ,Segurança Alimentar ,Risk analysis (engineering) ,Alimentation et Nutrition ,nutrition infantile ,business ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Relevant information ,Food Science - Abstract
29th International Conference of the European-Federation-of-Food-Science-and-Technology (EFFoST) -- NOV 10-12, 2015 -- Athens, GREECE, WOS: 000395217000007, Background: In vitro digestion models show great promise in facilitating the rationale design of foods. This paper provides a look into the current state of the art and outlines possible future paths for developments of digestion models recreating the diverse physiological conditions of specific groups of the human population. Scope and approach: Based on a collective effort of experts, this paper outlines considerations and parameters needed for development of new in vitro digestion models, e.g. gastric pH, enzymatic activities, gastric emptying rate and more. These and other parameters are detrimental to the adequate development of in vitro models that enable deeper insight into matters of food luminal breakdown as well as nutrient and nutraceutical bioaccessibility. Subsequently, we present an overview of some new and emerging in vitro digestion models mirroring the gastro-intestinal conditions of infants, the elderly and patients of cystic fibrosis or gastric bypass surgery. Key findings and conclusions: This paper calls for synchronization, harmonization and validation of potential developments in in vitro digestion models that would greatly facilitate manufacturing of foods tailored or even personalized, to a certain extent, to various strata of the human population. (C) 2016 Elsevier Ltd. All rights reserved., European Federat Food Sci & Technol
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- 2017
5. INFOGEST static in vitro simulation of gastrointestinal food digestion
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Frédéric Carrière, Torsten Bohn, Carla Martins, Adam Macierzanka, Didier Dupont, Werner Weitschies, Sébastien Marze, Ricardo Assunção, Alfonso Clemente, André Brodkorb, Uri Lesmes, Reto Portmann, Cláudia N. Santos, Isabelle Souchon, Milena Corredig, Claire Dufour, David Julian McClements, Claire Bourlieu-Lacanal, Rachel Boutrou, Martin S. J. Wickham, Paula Alvito, Olivia Ménard, Alan R. Mackie, Isidra Recio, Simon Ballance, Catharina Edwards, Gerd E. Vegarud, Mans Minekus, R. Paul Singh, Lotti Egger, Sibel Karakaya, Marie Alminger, Matt Golding, Steven Le Feunteun, Bente Kirkhus, Teagasc Food Research Centre [Fermoy, Ireland], Agroscope, Department of Biology and Biological Engineering, Chalmers University of Technology, Chalmers University of Technology [Gothenburg, Sweden], Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Nofima AS, Luxembourg Institute of Health, Luxembourg Institute of Healt, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Estación Experimental del Zaidín (EEZ), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Department of Food Science [Copenhagen] (UCPH FOOD), Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Quadram Institute Bioscience, Riddet Institute, Department of Food Engineering, Faculty of Engineering, Ege university, Israel Institute of Technology Technion City, Technion City, Faculty of Chemistry, Taras Shevchenko National University of Kyiv, School of Food Science & Nutrition, University of Leeds, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Chenoweth Lab, Department of Food Science, University of Massachusetts System (UMASS), Triskelion, Instituto de Biologia Experimental e Tecnológica (IBET), Instituto de Tecnologia Química e Biológica, Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Departments of Biological and Agricultural Engineering, and of Food Science and Technology, University of California [San Diego] (UC San Diego), University of California-University of California, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Reacta Biotech Limited, Ernst Moritz Arndt, Universität Greifswald - University of Greifswald, Instituto de Investigación en Ciencias de la Alimentación CIAL, CSIC-UAM, CEI UAM+CSIC (CIAL, CSIC-UAM, CEI UAM+CSIC), COST action FA1005 INFOGEST, Teagasc Food Research Centre [Fermoy, County Cork, Ireland], Chalmers University of Technology, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge I.P., Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Department of Physiology and Biochemistry of Animal Nutrition, Estacion Experimental del Zaidin, Department of Food Science, University of Guelph, Food Engineering Department, Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Department of Biotechnology and Food Engineering, Israel Institute of Technology, Institute of Food Research, Institute of Food Research [Norwich], Université de Minho, Portugal, Laboratoire de Physique des Solides (LPS), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), University of Massachusetts [Amherst] (UMass Amherst), Human Language Technology Interest Group (HULTIG), Universidade de Beira Interior, Instituto de Investigaciòn en Ciencias de la Alimentaciòn (CIAL, CSIC-UAM), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA), National Institute of Health Doutor Ricardo Jorge, Lisbon and CESAM, University of Aveiro, Estación Experimental del Zaidin, Consejo Superior de Investigaciones Científicas (CSIC), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (France), French National Institute for Agricultural Research, European Cooperation in Science and Technology (COST), FA1005, Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Norwegian Institute of Food,Fisheries and Aquaculture Research (NOFIMA), University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH), Quadram Institute Bioscience [Norwich, U.K.] (QIB), Biotechnology and Biological Sciences Research Council (BBSRC), University of California (UC)-University of California (UC), and Ege Üniversitesi
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Biomimetic materials ,[SDV]Life Sciences [q-bio] ,Oligosaccharides ,simulation models ,static model ,Matrix (chemical analysis) ,structure de l'aliment ,Human health ,Eating ,0302 clinical medicine ,static in vitro digestion ,Biomimetic Materials ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Bile ,Food science ,Amino Acids ,ComputingMilieux_MISCELLANEOUS ,2. Zero hunger ,0303 health sciences ,Gastric Juice ,Chemistry ,Hydrolysis ,Fatty Acids ,Stomach ,Food Ingredients ,santé humaine ,Hydrogen-Ion Concentration ,Intestines ,nutrition ,Avaliação do Impacte em Saúde ,physiological digestion ,Digestion ,modèle de simulation ,Models, Biological ,allégations nutritionnelles et de santé ,General Biochemistry, Genetics and Molecular Biology ,modèle statique ,digestion gastrointestinale ,03 medical and health sciences ,modèle statique un vitro ,simulated digestion ,Upper gastrointestinal ,Humans ,Gastric lipase ,Food structure ,Saliva ,030304 developmental biology ,Enzyme Assays ,cinétique de digestion ,Mouth ,composition de l'aliment ,digestion gastrique in vitro ,Intestinal digestion ,food digestion ,Peptide Fragments ,Composição dos Alimentos ,micronutriment ,Food ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,030217 neurology & neurosurgery - Abstract
WOS: 000462482100001, PubMed ID: 30886367, Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in similar to 7 d, including similar to 5 d required for the determination of enzyme activities., COST actionEuropean Cooperation in Science and Technology (COST) [FA1005 INFOGEST26]; French National Institute for Agricultural Research (INRA)Institut National de la Recherche Agronomique (INRA), We acknowledge COST action FA1005 INFOGEST26 (http://www.cost-infogest.eu/) for providing funding for travel, meetings and conferences (2011-2015). We acknowledge the French National Institute for Agricultural Research (INRA, http://www.inra.fr) for its continuous support of the INFOGEST network by organizing and co-funding the International Conference on Food Digestion and workgroup meetings. We thank A.G.F. Lopes (Universidade de Lisboa, Portugal) and V.S.N. Mishra (Teagasc Food Research Centre, Moorepark, Ireland) for their help in the final preparation of the videos. We also acknowledge the many other researchers, mostly associated with the above COST action and subsequent events, who have contributed to the discussion of digestion parameters.
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- 2019
6. Patulin and ochratoxin A co-occurrence and their bioaccessibility in processed cereal-based foods: A contribution for Portuguese children risk assessment
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Paula Alvito, Carla Martins, Didier Dupont, Ricardo Assunção, Food and Nutrition Department, National Institute of Health Dr. Ricardo Jorge, CESAM, Centre for Environmental and Marine Studies, Universidade de Aveiro, IIFA, Universidade de Évora, Centre for Environmental and Marine Studies [Aveiro] (CESAM), University of Aveiro, National School of Public Health, NOVA University of Lisboa, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, project MYCOMIX (PTDC/ DTP-FTO/0417/2012) and through CESAM (UID/AMB/50017/2013) both funded by the Fundaçao para a Ciencia e Tecnologia, (Portugal). The authors would like to thanks to COST action FA1005 INFOGEST, Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Centro de Estudos do Ambiante e do Mar (CESAM), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
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Ochratoxin A ,Bioaccessibility ,Toxicology ,01 natural sciences ,Ochratoxins ,Patulin ,Eating ,chemistry.chemical_compound ,céréale ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Medicine ,Standardised in vitro digestion ,Lack of knowledge ,Food science ,Children ,bioaccessibilité ,2. Zero hunger ,food and beverages ,patuline ,04 agricultural and veterinary sciences ,General Medicine ,santé humaine ,040401 food science ,3. Good health ,Child, Preschool ,contamination des aliments ,Risk assessment ,animal structures ,mycotoxine ,Food Contamination ,Avaliação de Risco ,Risk Assessment ,0404 agricultural biotechnology ,Saúde Humana ,Humans ,Vulnerable population ,Toxicologia ,Mycotoxin ,Portugal ,business.industry ,010401 analytical chemistry ,Infant, Newborn ,Infant ,Mycotoxins ,0104 chemical sciences ,Biotechnology ,body regions ,Segurança Alimentar ,chemistry ,Carcinogens ,Edible Grain ,business ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Mutagens ,Food Science ,Food contaminant - Abstract
Patulin (PAT) and ochratoxin A (OTA) are well known enteropathogenic mycotoxins that are present in several foodstuffs. Processed cereal-based foods are among the first solid foods eaten by children, a particularly vulnerable population group. There is a lack of knowledge related to the co-occurrence of PAT and OTA in food intended for children consumption and their potential interactions during the digestion process. The present study aims to evaluate, for the first time, the co-occurrence of PAT and OTA in processed cereal-based foods for children consumption, the bioaccessibility of these two mycotoxins, and the contribution of the bioaccessibility data for human health risk assessment. PAT and OTA incidence were 75% and 50%, respectively. These mycotoxins co-occurred in 40% of analysed samples. Bioaccessibility assays revealed mean values of 52% and 56% for PAT, alone and combined with OTA; and 100% and 106% for OTA, alone and combined with PAT. Considering the human health risk assessment, and taking into account the co-occurrence and the bioaccessibility results, this study indicates a tolerable exposure to these mycotoxins representing a low risk for Portuguese children. The present work reinforces the importance of a holistic approach for risk assessment which gathers data from occurrence, exposure and bioaccessibility. This research was supported by the project MYCOMIX (PTDC/DTP-FTO/0417/2012) and through CESAM (UID/AMB/50017/2013) both funded by the Fundação para a Ciência e Tecnologia, Portugal. The authors would like to thanks to COST action FA1005 INFOGEST for the support and training in standardised static in vitro digestion method and to Dr. Elsa Vasco for advice and support regarding children food consumption. info:eu-repo/semantics/publishedVersion
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- 2016
7. MYCOMIX and INFOGEST: a fruitful collaboration
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Alvito, P., Assuncao, R., Martins, C., Tavares, A., Egger , C., Lea, T., Dupont, Didier, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge I.P, Centre for Environmental and Marine Studies (CESAM), Faculty of Sciences, University of Lisbon, Universidate de Evora, Agroscope, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, COST Action FA 1005 INFOGEST, Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Universidade de Lisboa (ULISBOA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Institut National de Recherche Agronomique (INRA). UMR Science et Technologie du Lait et de l'Oeuf (1253).
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Segurança Alimentar ,contamination ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,mycotoxine ,Saúde Humana ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,aliment ,toxicité ,Toxicologia ,santé humaine ,Avaliação de Risco ,Genotoxicidade Ambiental ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition - Abstract
This research was performed under the MycoMix project (PTDC/DTP-FTO/0417/2012) funded by the Fundação para a Ciência e Tecnologia (FCT), Portugal.; Mycotoxins are natural contaminants produced by fungi and its common occurrence in food poses a threat to human health, mainly to vulnerable population groups as children. The MycoMix Project (2013-15) aims to study the occurrence of multiple mycotoxins and toxicity interactions in infant foods and cereals consumed by Portuguese children and try to answer several questions: 1) Are children exposed daily to mycotoxins through food? 2) What are the quality and quantity that characterize this exposure? 3) Can this exposure bring harm to children? In an attempt to elucidate the internal mycotoxin dose that will reach the intestinal tract and can produce toxic effects, several bioavailability studies as well as dissemination activities were performed under collaboration between MYCOMIX and INFOGEST. This fruitful cooperation resulted in: i) two STSMs at laboratories of INFOGEST members: Agroscope (Switzerland) and Norwegian University of Life Sciences (Norway), on models of gastrointestinal digestion (WG2) and effect of food on health (WG3), respectively, ii) 2 publications in peer-reviewed journals and 2 national MSc dissertations using in vitro digestion models, iii) 7 communications in international and national meetings, iv) organization of a WG meeting in Lisbon, v) collaboration in the 1st WG2 technical training session, vi) attendance of 2 PhD students to training school ”Food Digestion and Human Health”, vii) dissemination of the INFOGEST network as well as its standardized in vitro digestion method in national and international meetings promoted by MYCOMIX (http://www.insa.pt/sites/INSA/Portugues/ComInf/Noticias/Paginas/1_SN_PAS.aspx, November 2014; http://www.icfc2015.com/welcome.htm, April 2015) and in one international post-graduation course in Brasil12, and finally, viii) production of 6 videos concerning the in vitro harmonized food digestion method, set up by members of the WG2 of EU-COST Action (www.youtube.com/channel/UCdc-NPx9kTDGyH_kZCgpQWg).
- Published
- 2015
8. Neuroinflammation, mitochondrial defects and neurodegeneration in mucopolysaccharidosis III type C mouse model
- Author
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David Cheillan, Helena Hůlková, Lubov S. Grigoryeva, Alain Moreau, Larbi Dridi, M. Hrebicek, Brian W. Bigger, Alex Langford-Smith, Zuzana Hájková, Alexey V. Pshezhetsky, Edith Hamel, Virginie Dormoy-Raclet, Kazuhiro Ohmi, Markéta Tesařová, Eva Svobodová, Jérôme Ausseil, Carla Martins, Hana Hansikova, Yoo Choi, Fiona L. Wilkinson, Graziella DiCristo, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge I.P, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National de la Recherche Agronomique (INRA)
- Subjects
medicine.medical_specialty ,Mucopolysaccharidosis ,medicine.medical_treatment ,[SDV]Life Sciences [q-bio] ,Biology ,Mitochondrion ,Inbred C57BL ,Gangliosides/metabolism ,Energy Metabolism/physiology ,Mice ,Proteostasis Deficiencies/pathology ,Mucopolysaccharidosis III ,Internal medicine ,medicine ,Animals ,Mitochondrial Diseases/etiology/*pathology ,Neurodegenerative Diseases/etiology/*pathology/psychology ,Glycosaminoglycans/metabolism ,Neuroinflammation ,Acetyltransferases/deficiency/genetics ,Neurologic Examination ,Behavior ,Microglia ,Catabolism ,Animal ,Neurodegeneration ,Original Articles ,medicine.disease ,3. Good health ,Cytokine ,Endocrinology ,medicine.anatomical_structure ,Mucopolysaccharidosis III/complications/*pathology/psychology ,Immunology ,Microtubule-Associated Proteins/metabolism ,Neurology (clinical) ,Neuritis/etiology/*pathology - Abstract
International audience; Severe progressive neurological paediatric disease mucopolysaccharidosis III type C is caused by mutations in the HGSNAT gene leading to deficiency of acetyl-CoA: alpha-glucosaminide N-acetyltransferase involved in the lysosomal catabolism of heparan sulphate. To understand the pathophysiology of the disease we generated a mouse model of mucopolysaccharidosis III type C by germline inactivation of the Hgsnat gene. At 6-8 months mice showed hyperactivity, and reduced anxiety. Cognitive memory decline was detected at 10 months and at 12-13 months mice showed signs of unbalanced hesitant walk and urinary retention. Lysosomal accumulation of heparan sulphate was observed in hepatocytes, splenic sinus endothelium, cerebral microglia, liver Kupffer cells, fibroblasts and pericytes. Starting from 5 months, brain neurons showed enlarged, structurally abnormal mitochondria, impaired mitochondrial energy metabolism, and storage of densely packed autofluorescent material, gangliosides, lysozyme, phosphorylated tau, and amyloid-beta. Taken together, our data demonstrate for the first time that deficiency of acetyl-CoA: alpha-glucosaminide N-acetyltransferase causes lysosomal accumulation of heparan sulphate in microglial cells followed by their activation and cytokine release. They also show mitochondrial dysfunction in the neurons and neuronal loss explaining why mucopolysaccharidosis III type C manifests primarily as a neurodegenerative disease.
- Published
- 2015
9. Applicability of in vitro methods to study patulin bioaccessibility and its effects on intestinal membrane integrity
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Mauro Bragança, Paula Alvito, Ricardo Assunção, Didier Dupont, Carla Martins, Irene Diaz, Beatriz Padilla, Mariana Ferreira, Food and Nutrition Department, Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), University of Évora [Portugal], Faculty of Sciences, Universidade de Lisboa (ULISBOA), Division of Toxicology, Wageningen University and Research [Wageningen] (WUR), Universitat Politècnica de València (UPV), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Faculty of Veterinary Medicine, Lusófona University of Humanities and Technology, National Institute of Health Doutor Ricardo Jorge I.P., University of Lisbon, Wageningen University and Research Centre [Wageningen] (WUR), and Universitat Politecnica de Valencia (UPV)
- Subjects
Cell Membrane Permeability ,Health, Toxicology and Mutagenesis ,Biological Availability ,Absorption (skin) ,010501 environmental sciences ,Biology ,Toxicology ,01 natural sciences ,modéle ,Models, Biological ,Risk Assessment ,Intestinal absorption ,Patulin ,mycoyoxine ,chemistry.chemical_compound ,0404 agricultural biotechnology ,cystéine ,adsorption intestinale ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Ingestion ,Humans ,Food science ,Toxicologia ,Cysteine ,Mycotoxin ,0105 earth and related environmental sciences ,patuline ,04 agricultural and veterinary sciences ,santé humaine ,040401 food science ,In vitro ,Intestines ,Segurança Alimentar ,chemistry ,Caco-2 ,Modelos de Digestão in vitro ,Digestion ,Caco-2 Cells ,contaminant ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition - Abstract
In human health risk assessment, ingestion of food is considered a major route of exposure to many contaminants, although the total amount of an ingested contaminant (external dose) does not always reflect the quantity available for the body (internal dose). In this study, two in vitro methods were applied to study bioaccessibility and intestinal membrane integrity of cells exposed to patulin, a mycotoxin with significant public health risk. Seven artificially contaminated fruit juices were assayed in the presence or absence of a standard meal, showing a significant difference for bioaccessibility values between contaminated samples alone (mean 27.65 ± 13.50%) and combinations with a standard meal (mean 7.89 ± 4.03%). Different concentrations of patulin (PAT) and cysteine (CYS) (protector agent) were assayed in Caco-2 cells monolayers. At 95 μM, PAT produced a marked decrease in transepithelial electrical resistance (TEER). This effect was significantly reduced when 400 μM and 4000 μM CYS was added to the cells. Combined use of in vitro digestion models with other techniques using intestinal cell lines, such as in vitro intestinal absorption models that use Caco-2 cells, may offer a more comprehensive model of what is occurring during digestion and absorption processes. The study of beneficial effects of protective agents would also be enhanced. FCT
- Published
- 2014
10. Functional magnetic resonance imaging suggests automatization of the cortical response to inspiratory threshold loading in humans
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Mathieu Raux, Eric Bardinet, Carine Karachi, Thomas Similowski, Felix Kindler, Lisa Koski, Louise Tyvaert, Michael Ferreira, Capucine Morélot-Panzini, G. Bruce Pike, Jean Gotman, CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Food and Nutrition Department, Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Faculty of Sciences, Universidade de Lisboa = University of Lisbon (ULISBOA), Center for NeuroImaging Research-Human MRI Neuroimaging core facility for clinical research [ICM Paris] (CENIR), Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Neurophysiologie Respiratoire Expérimentale et Clinique, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), EEG Department, McGill University = Université McGill [Montréal, Canada]-Montreal Neurological Institute and Hospital, McGill University = Université McGill [Montréal, Canada], Service d'Accueil des Urgences [CHU Pitié-Salpêtrière], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Universidade de Lisboa (ULISBOA), Service de Pneumologie et Réanimation Médicale [CHU Pitié-Salpêtrière] (Département ' R3S '), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Pierre et Marie Curie - Paris 6 (UPMC), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Neuro-Imagerie de Recherche (CENIR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Pitié-Salpêtrière [AP-HP]
- Subjects
Pulmonary and Respiratory Medicine ,Adult ,Male ,Physiology ,Physical Exertion ,Differential Threshold ,Reticular formation ,behavioral disciplines and activities ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,medicine ,Image Processing, Computer-Assisted ,Tidal Volume ,Humans ,Default mode network ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,Cerebral Cortex ,0303 health sciences ,Brain Mapping ,Blood-oxygen-level dependent ,Supplementary motor area ,medicine.diagnostic_test ,General Neuroscience ,[SCCO.NEUR]Cognitive science/Neuroscience ,Brain ,Overlearning ,Magnetic Resonance Imaging ,Respiration, Artificial ,Oxygen ,medicine.anatomical_structure ,Inhalation ,Cerebral cortex ,Female ,Psychology ,Functional magnetic resonance imaging ,Insula ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Inspiratory threshold loading (ITL) induces cortical activation. It is sustained over time and is resistant to distraction, suggesting automaticity. We hypothesized that ITL-induced changes in cerebral activation may differ between single-breath ITL and continuous ITL, with differences resembling those observed after cortical automatization of motor tasks. We analyzed the brain blood oxygen level dependent (BOLD) signal of 11 naive healthy volunteers during 5 min of random, single-breath ITL and 5 min of continuous ITL. Single-breath ITL increased BOLD in many areas (premotor cortices, bilateral insula, cerebellum, reticular formation of the lateral mesencephalon) and decreased BOLD in regions colocalizing with the default mode network. Continuous ITL induced signal changes in a limited number of areas (supplementary motor area). These differences are comparable to those observed before and after overlearning of motor tasks. We conclude that the respiratory-related cortical activation observed in response to ITL is likely due to automated, attention-independent mechanisms. Also, ITL activates cortical circuits right from the first breath.
- Published
- 2013
11. In Acknowledgement of the Review: Perfection Salad-Women and Cooking at the Turn of the Century
- Author
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Reiko, MARUSHIMA, 書評, Book Review, 神戸女学院大学家政学部食物学科(家政学原論), and Associate Professor of Kobe College, Food and Nutrition Department(Philosophy of Home Economics)
- Published
- 1992
12. Perfection Salad-Women and Cooking at the Turn of the Century by Laura Shapiro
- Author
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Yutaka, SHIMIZU, 書評, Book Review, 神戸女学院大学家政学部食物学科非常勤, 神戸学院女子短期大学(食品化学), and Part-time lecturer at Kobe College, Food and Nutrition Department/Professor of Kobe-gakuin Women's Jr.College(Food Chemistry)
- Published
- 1992
13. In vitro impact of a whey protein isolate (WPI) and collagen hydrolysates (CHs) on B16F10 melanoma cells proliferation
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Georgia Alvares de Castro, Valdemiro Carlos Sgarbieri, Saïd Bouhallab, Durvanei Augusto Maria, Food and Nutrition Department, Food Engineering Faculty, Universidade Estadual de Campinas (UNICAMP), Biochemistry and Biophysics Laboratory, Instituto Butantan, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
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Skin Neoplasms ,Protein Hydrolysates ,Swine ,[SDV]Life Sciences [q-bio] ,Biochemistry ,Whey protein isolate ,Mice ,0302 clinical medicine ,protéine du lait ,Melanoma ,0303 health sciences ,biology ,Lactoferrin ,Chemistry ,Caspase 3 ,Cell Cycle ,Milk Proteins ,B16F10 MELANOM ,3. Good health ,APOPTOSIS ,protéine ,030220 oncology & carcinogenesis ,Collagen ,Programmed cell death ,Cell Survival ,Antineoplastic Agents ,Dermatology ,Hydrolysate ,WHEY PROTEIN ,03 medical and health sciences ,Inhibitory Concentration 50 ,milk protein ,Cell Line, Tumor ,Cell Adhesion ,Animals ,Molecular Biology ,030304 developmental biology ,Cell Proliferation ,apoptose ,Molecular mass ,CELL CYCLE PHASES ,In vitro ,COLLAGEN HYDROLYSATES ,Whey Proteins ,Cell culture ,Apoptosis ,biology.protein ,Gelatin ,Cattle ,protein - Abstract
Background Porcine skin gelatine presented anti-tumoral effect on murine hepatoma cells (MH134), inducing programmed cell death (apoptosis). Whey proteins (mainly lactoferrin) have been investigated for cancer prevention and treatment. Objective Investigation of the inhibitory capacity on melanoma cells (B16F10) proliferation and the influence on % distribution of cell cycle phases, in the presence of various concentrations of whey protein isolate (WPI), bovine collagen hydrolysate (BCH) or its fractions. Methods The permeate fraction BCH-P1 (molecular mass, MM 2.5kDa) was further fractionated into five retentate fractions (R1–R5) by ultrafiltration membranes and into four fractions (F1–F4) by reverse phase chromatography. The permeate BCH-P1 and all its fractions were comparatively tested against a negative control (B16F10 cells+culture medium), and also against a positive control (B16F10+culture medium+WPI). Results The inhibitory concentrations for 50% of B16F10 cells (IC 50 ) ranged from 0.19 to 156.9mg/mL for all these proteins evaluated. The most inhibitory fractions of the BCH hydrolysate were BCH-P1 and F1–F4 with IC 50 concentrations below 1mg/mL. Changes in cell cycle phases were characterized by a general decrease in the G2/M phase that emphasizes growth arrest, some increase in phase S (BCH-P1 and F4) but a strong increase in G0/G1 phase for BCH-P1 and F4. Caspase-3 expression increased significantly in all media containing F and R fractions, and also in the presence of BCH or WPI. Apoptosis was extremely high at low concentration (400μg/mL) of the F1–F3 fractions. Conclusion It is suggested that a mechanism for tumorigenesis inhibition may involve the caspases cascade and apoptosis.
- Published
- 2009
14. アンケート ホウコク コウベジョガクイン コウトウガクブ 3ネンセイ ノ セイカツ イシキ ニ ツイテ
- Author
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Keiko, OKAMOTO, 論文, Article, 神戸女学院大学家政学部食物学科(食物学), and Associate Professor of Kobe College, Food and Nutrition Department(Food Science)
- Subjects
education - Abstract
In October,1987,we surveyed 130 senior year high school girls at Kobe College concerning school life, future after gradution, views on career,marriage, men and women, and women's life style by questionnaire. Here are some interesting findings. 1)Eighty percent of them were satisfied with high school life. 2)All of them wanted to pursue further studies after graduation.Ninety percent wanted to graduate from a 4-year college over. 3)Seventy percent wanted to pursue a career in which they could use their abilities the best. Forty percent did not know what career they wanted to pursue. The rest wanted to be teachers or research workers doing work that requires skill, a degree and experience which are evaluated highly. Sixty percent of the students wanted to be able to work equally as men without any sexual harassment. 4)Eighty percent considered marriage positively. The ideal marriage in their view was a marriage as a partner or friend. 5) The favored male and female views were drawn. Sixty percent of them were satisfied as to have been born female. 6) The most accepted blueprint for life was temporary leave from career and return(40%). and terminating career life with prospect of pregnancy(33%). This was followed by continuation without career interruption(30%). Nineteen percent concluded that they would like to continue working without marrying. Sixty-four percent considered raising children. Ninety percent of them wanted to pursue some kind of career after graduation. Thirty percent wanted to continue work for life.
- Published
- 1990
15. Copper biomarkers and their relationship with parameters of insulin resistance in obese women.
- Author
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da Silva NC, da Silva LD, Chaves BMS, de Oliveira EHS, Barros EML, de Oliveira FE, Henriques GS, Pereira IC, Severo JS, Dos Santos LR, de Moura MSB, da Cunha Soares T, de Sousa TGV, de Sousa Melo SR, and do Nascimento Marreiro D
- Abstract
Some studies have demonstrated the involvement of high concentrations of copper in the manifestation of insulin resistance in individuals with obesity. The objective of this study was to evaluate the copper nutritional status and its relationship with parameters of glycemic control in women with obesity. An observational case-control clinical study involving 203 women aged between 20 and 50 years, divided into two groups: obese (n = 84) and eutrophic (n = 119). Body weight, height and waist, hip and neck circumferences, dietary copper intake, copper biomarkers, determine ceruloplasmin activity and glycemic control parameters were measured. It was observed that women with obesity had higher copper concentrations in plasma and lower concentrations in erythrocytes when compared to the control group. Analysis of glycemic control parameters revealed a statistically significant difference in fasting blood glucose (p < 0.05) between groups. The study identified a significant positive correlation between plasma copper and fasting insulin values and the Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index (p < 0.05). The results of this study demonstrate that obese women have high copper concentrations in plasma and lower concentrations in erythrocytes. Furthermore, the significant positive correlation between plasma copper and fasting insulin and HOMA-IR index suggests the influence of this mineral on glycemic control parameters in obese women., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
- Published
- 2024
- Full Text
- View/download PDF
16. Visnagin alleviates rheumatoid arthritis via its potential inhibitory impact on malate dehydrogenase enzyme: in silico, in vitro, and in vivo studies.
- Author
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Khamis AA, Sharshar AH, Mohamed TM, Abdelrasoul EA, and Salem MM
- Abstract
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disorder. The present study aimed to evaluate the in silico, in vitro, and in vivo inhibitory effect of visnagin on malate dehydrogenase activity and elucidate its inflammatory efficacy when combined with methotrexate in the RA rat model. The molecular docking, ADMET simulations, MDH activity, expression, and X-ray imaging were detected. Moreover, CRP, RF, (anti-CCP) antibody, (TNF-α), (IL-6), (IL-17), and (IL-10) were evaluated. The expression levels of MMP3 and FOXP3 genes and CD4, CD25, and CD127 protein levels were assessed. Histological assessment of ankle joints was evaluated. The results revealed that visnagin showed reversible competitive inhibition on MDH with inhibitory constant (Ki) equal to 141 mM with theoretical IC50 equal to 1202.7 mM, LD50 equal to 155.39 mg/kg, and LD25 equal to 77.69 mg/kg. In vivo studies indicated that visnagin exhibited anti-inflammatory effects through decreasing MDH1 activity and expression and induced proliferation of anti-inflammatory CD4
+ CD25+ FOXP3 regulatory T cells with increasing the anti-inflammatory cytokine IL-10 levels. Moreover, visnagin reduced the levels of inflammatory cytokines and the immuno-markers. Our findings elucidate that visnagin exhibits an anti-inflammatory impact against RA through its ability to inhibit the MDH1 enzyme, improve methotrexate efficacy, and reduce oxidative stress., (© 2024. The Author(s).)- Published
- 2024
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17. Phthalocyanine derivative attenuates TNF-α production in macrophage culture and prevents alveolar bone loss in experimental periodontitis.
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Breseghello I, Rosalen PL, Bruzadelli RFD, de Araújo LP, Abdalla HB, Lazarini JG, Paiva IM, Bueno-Silva B, Cordeiro MR, de Alencar SM, Vilhena FV, Cunha TM, Fernandes LA, Ikegaki M, and Franchin M
- Abstract
Aim: This study investigated the activity and mechanism of action of the iron tetracarboxyphthalocyanine (FeTcPc) on tumor necrosis factor alpha (TNF-α) production and its impact on experimental periodontitis., Methods: RAW 264.7 macrophages were treated with FeTcPc, activated with lipopolysaccharide (LPS) at 10 ng/mL, and the TNF-α levels were measured, as well as the nuclear factor kappa B (NF-κB) activation. Subsequently, a mouth gel containing 1% FeTcPc was topically administered to the gingival tissue of mice with periodontitis-induced ligatures. Bone loss and the gene expression of Tnfα, p65 (NF-κB), and receptor-activating nuclear factor kappa B ligand (Rankl) were quantified in gingival tissue. Finally, the systemic toxicity of FeTcPc was estimated in Galleria mellonella larvae., Results: In an activated RAW 264.7 macrophage culture, 100 μM FeTcPc reduced TNF-α release and NF-κB activation. Regarding experimental periodontitis, topical application of mouth gel containing 1% FeTcPc blocked alveolar bone loss. Additionally, 1% FeTcPc reduced the expression of Tnfα, p65 (NF-κB), and Rankl in gingival tissue. Finally, administration FeTcPc at doses ranging from 1 to 1000 mg/kg did not cause acute systemic toxicity in G. mellonella., Conclusion: Overall, we demonstrated the potential of mouth gel containing FeTcPc as a therapeutic strategy for managing osteolytic inflammatory disorders, such as periodontitis., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2024
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18. Novel composite of nano zinc oxide and nano propolis as antibiotic for antibiotic-resistant bacteria: a promising approach.
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Salama SA, Essam D, Tagyan AI, Farghali AA, Khalil EM, Abdelaleim YF, Hozzein WN, Mubarak M, Nasr FA, Eweis AA, Al-Zharani M, and Mahmoud R
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- Pseudomonas aeruginosa drug effects, Drug Resistance, Bacterial drug effects, Humans, Spectroscopy, Fourier Transform Infrared, Metal Nanoparticles chemistry, Zinc Oxide chemistry, Zinc Oxide pharmacology, Propolis chemistry, Propolis pharmacology, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Nanocomposites chemistry, Microbial Sensitivity Tests
- Abstract
This study proposes an innovative approach to combat the escalating threat of antibiotic resistance in bacteria by introducing a novel ZnO-propolis nanocomposite (ZnO-P NCs). The overuse of antibiotics, particularly during events like the COVID-19 pandemic, has intensified bacterial resistance, necessitating innovative solutions. The study employs a cost-effective and controllable biosynthesis method to produce ZnO nanoparticles (ZnO-NPs), with propolis extract crucially contributing to the reduction and stabilization of Zn
2+ ions. A biodegradable nano-propolis matrix is then created by incorporating ZnO-NPs, forming the ZnO-P NCs. Structural stability is confirmed through FT-IR and Zeta potential analysis, while nanoscale properties are validated via TEM, SEM, and XRD analyses. The antimicrobial efficacy of various substances, including propolis, nano propolis, ethanolic propolis extract, ZnO-NPs, and ZnO-P NCs, is assessed against Gram-negative and Gram-positive bacteria, alongside a comparison with 28 antibiotics. Among the bacteria tested, Pseudomonas aeruginosa PAO1 ATCC15692 was more sensitive (40 mm) to the biosynthesized nanocomposite ZnO-P NCs than to ZnO-NPs (38 mm) and nanopropolis (32 mm), while Escherichia coli was resistant to nanopropolis (0 mm) than to ZnO-NPs (31 mm), and ZnO-P NCs (34 mm). The study reveals a synergy effect when combining propolis with green-synthesized ZnO-NPs in the form of ZnO-P NCs, significantly improving their efficiency against all tested bacteria, including antibiotic-resistant strains like E. coli. The nanocomposite outperforms other materials and antibiotics, demonstrating remarkable antibacterial effectiveness. SEM imaging confirms the disruption of bacterial cell membranes by ZnO-NPs and ZnO-P NCs. The study emphasizes the potential applications of ZnO-NPs integrated into biodegradable materials and underscores the significance of the zinc oxide-propolis nanocomposite in countering antimicrobial resistance. Overall, this research offers a comprehensive solution to combat multidrug-resistant bacteria, opening avenues for novel approaches in infection control., (© 2024. The Author(s).)- Published
- 2024
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19. Feeding practices and dietary intake in Brazilian children with Down syndrome: A cross-sectional study.
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Genovesio MCRS, Monteiro LS, da Silva AV, Rodrigues PRM, Baumblatt AP, and Ribas SA
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- Humans, Male, Brazil epidemiology, Cross-Sectional Studies, Female, Infant, Child, Preschool, Diet statistics & numerical data, Down Syndrome epidemiology, Feeding Behavior physiology
- Abstract
Background: Studies investigating the quality of the diet and dietary intake of children with Down syndrome (DS) are required because the features attributed to the syndrome can affect growth, development and quality of life., Methods: This cross-sectional study was conducted with 77 Brazilian children with DS between 5 and 36 months of age receiving care at the multidisciplinary outpatient clinic of the University Hospital. Participants' sociodemographic, dietary and anthropometric data were collected from the care protocols. Dietary data were collected from 24-h recalls and dietary practices were assessed according to the WHO dietary guidelines. Associations between inadequate feeding practices and demographic variables were assessed using logistic regression models., Results: Fruits, milk or infant formula, vegetables, beans and meat were among the five most consumed foods by the children investigated. Overall, we observed a high number of cases of early weaning (50.6%), low minimum dietary diversity (MDD; 40.3%), inadequate consistency for age (64.9%), early presence of ultra-processed foods (76.6%), sugars and sweets (33.8%) in the diet of the children with DS. In the associations of inadequate feeding practices by age group, low MDD [odds ratio (OR): 18.6; 95% confidence interval (CI): 3.4; 57.1] and inadequate consistency (OR: 6.65; 95% CI: 1.8; 24.7) were more frequent among children aged below 12 months while this relationship was inverse for early introduction of sugar and sweets (OR: 0.04; 95% CI: 0.01; 0.29)., Conclusion: Our findings showed a high number of cases of inadequate dietary practices in children with DS investigated, which could adversely affect the long-term health of this population., (© 2024 John Wiley & Sons and MENCAP.)
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- 2024
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20. Microbiological contamination present in enteral tube feeding prepared in hospitals and/or at home: a systematic review.
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Kozow JFC, Rabito EI, Kruger JF, Medeiros CO, da Costa RL, Beux MR, and Stangarlin-Fiori L
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- Humans, Food Microbiology, Food Contamination analysis, Food Contamination prevention & control, Hospitals, Food, Formulated microbiology, Enteral Nutrition methods
- Abstract
Context: The safety of enteral formulas is important to restore and maintain the health of patients., Objective: A systematic review of the literature was conducted to assess the microbiological contamination present in enteral tube feeding prepared in hospitals and/or at home., Data Sources: A systematic search was conducted of the Medline, Scopus, BVS, CAPES/MEC, Embase, Science Direct, and SciELO databases and gray literature., Data Extraction: Eligible studies that analyzed the contamination of enteral formulas manipulated in hospitals and/or at home were selected; a quality assessment tool was used., Data Analysis: Twenty-three studies evaluated 1099 enteral formulations. Of these, 44.67% of enteral formulas (n = 491) exceeded the acceptable bacterial count. Samples of homemade enteral formulation preparations (86.03%; n = 191) had the highest bacterial counts, followed by mixed preparations (79.72%; n = 59), and commercial formulas (30.01%; n = 241). The number of samples of enteral formulations that exceeded the bacterial count at home was 70.79% (n = 160 at the hospital was 37.91% (n = 331). Total coliforms (82.68%; n = 406) and mesophilic aerobes (79.22%; n = 389) were the most common microorganisms. Samples with bacterial pathogens were also identified, with Bacillus cereus (4.07%; n = 20) and Listeria monocytogenes (3.66%; n = 18) being the most prevalent., Conclusions: A high number of samples of enteral formulations exceeded the bacterial count, but the risk to patient's health when consuming enteral tube feeding prepared in hospitals or at home may be low. This is because the bacteria present in the samples are not considered potential causes of disease but rather indicators of hygiene conditions., Systematic Review Registration: PROSPERO registration no. CRD42022367573., (© The Author(s) 2023. Published by Oxford University Press on behalf of the International Life Sciences Institute. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2024
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21. Valorisation of sea urchin (Paracentrotus lividus) gonads through canning.
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Camacho C, Oliveira H, Serrano C, Delgado I, Coelho I, Pedro S, Lourenço H, Bandarra NM, Marques A, Pessoa MF, Gonçalves A, and Nunes ML
- Subjects
- Animals, Humans, Food Preservation methods, Carotenoids analysis, Carotenoids chemistry, Sea Urchins chemistry, Nutritive Value, Gonads chemistry, Gonads metabolism, Paracentrotus chemistry
- Abstract
Fresh sea urchin (Paracentrotus lividus) gonads are a delicacy with short seasonal availability, very often heterogeneous in size and intrinsic characteristics. This study aimed to valorise this resource through the preparation of canned products (with/without Porphyra spp.) and evaluate their physicochemical and sensory quality (3-12 months). Canning contributed to a decrease in protein, K and most carotenoids contents; and a concentration of lipids, ash, Na and Se levels. A simulated 12-month ageing led to decrease the protein and β-carotene contents; and the Na and lutein levels concentration. The macroalgae addition resulted in an orange, darker and less soft product, with higher carbohydrates, Na, Se and carotenoids contents. A 25 g-dose contributes to significant daily intakes of protein (8-9%), EPA+DHA (47-53%), I (35-62%) and Se (30-47%). The products were commercially stable/sterile and had good sensory acceptance. Overall, canning constitutes a strategy to provide a nutritionally balanced product available all year-round., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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22. Commercial propolis extracts from Apis mellifera, Scaptotrigona bipunctata and Melipona quadrifasciata : quality control, antioxidant and antiglycemic potential.
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Dos Anjos RTKB, Engracia MJG, Etgeton SAP, Silva Rodrigues ADDP, Rodrigues Silva AC, Krüger CCH, Turnes Pasini Deolindo C, Hoff RB, Barreto F, Moresco KS, Ávila S, and Rodrigues Ferreira SM
- Abstract
Quality control of propolis plays a pivotal role in ensuring the appropriate concentrations of active compounds, limiting unwanted substances, verifying authenticity, and adhering to regulatory standards. This study aimed to assess the identity and quality standards, the individual phenolic composition (LC-ESI-MS/MS), and the antioxidant and antiglycemic potential of commercial propolis extracts (CPEs) from Apis mellifera , Scaptotrigona bipunctata , and Melipona quadrifasciata bees. CPEs met wax content and oxidation activity criteria, surpassing minimum thresholds for total phenolic content (TPC) and flavonoid content (TFC), although stingless bee CPE did not test positive for 10% lead acetate. CPEs exhibited antioxidant and potential antiglycemic activities. Epicatechin among the thirty-three identified phenolics, showed significant correlation with TPC, DPPH, ABTS, and EC50 values of α-amylase enzyme. These promising attributes underscore the potential health benefits of commercial propolis extracts from Apis mellifera and stingless bees for further medicinal and nutritional applications.
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- 2024
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23. Comparative analysis of nutritional quality and microbial contamination in camel milk: Evaluating safety and public health risks between camel milk obtained from street vendors and farms in Saudi Arabia.
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Abdulsalam NM and Alghamdi EM
- Subjects
- Animals, Saudi Arabia, Humans, Food Microbiology, Farms, Public Health, Staphylococcus aureus isolation & purification, Escherichia coli isolation & purification, Commerce, Food Safety, Food Contamination analysis, Camelus microbiology, Milk microbiology, Nutritive Value
- Abstract
Objectives: To evaluate the nutritional quality and microbial contamination of raw camel milk sourced from street vendors and compare it with milk obtained from farms, with a particular emphasis on pathogenic organisms., Methods: Twenty samples were systematically collected from street vendors and farms between July 2022 and February 2023 and analyzed at King Fahad Medical Research Centre and the Pharmacy College, King Abdulaziz University, Jeddah, Saudi Arabia. The microbial analysis employed culture-dependent techniques for colony-forming unit analysis and isolation of microbial colonies from milk samples. Microbial identification utilized advanced methods, including VITEK-MS equipment and the MALDI-TOF technique. The chemical composition was analysed through enzyme-linked immunosorbent assays., Results: The findings revealed significant differences in microbial loads, with milk sourced from street vendors exhibiting considerably higher microbial counts than farm-sourced milk, including pathogenic species like Staphylococcus aureus and Escherichia coli . The results indicated that camel milk from street vendors possessed a higher level of microbial contamination, suggesting potential health risks associated with its purchase and consumption from these sources., Conclusion: This study highlights the urgent need for stringent food safety practices in handling, selling, and distributing camel milk to reduce microbial risks to safe levels, thereby mitigating potential health hazards., (Copyright: © Saudi Medical Journal.)
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- 2024
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24. Assessment of the risk of contamination of enteral nutrition bottles based on the simulation of home use conditions and hygiene procedures.
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da Silva ITF, Medeiros CO, Leobet J, Beux MR, Rabito EI, Etgeton SAP, and Fiori LS
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- Humans, Detergents, Food Microbiology, Food, Formulated analysis, Food Packaging methods, Food Contamination analysis, Equipment Contamination prevention & control, Risk Assessment, Enteral Nutrition methods, Enteral Nutrition instrumentation, Hygiene
- Abstract
Background: Home-prepared enteral formulations are supplied to patients through enteral nutrition bottles, via a gravity bag or other container, which may be inadequately sanitized and reused more times than recommended by the manufacturer. Such procedures increase the risk of contamination and can compromise the patient's clinical outcome. In light of this, the present study aimed to assess the risk of contamination of enteral nutrition bottles by simulating home use conditions and hygiene procedures., Methods: A simulation of bottle usage was conducted across the three categories of enteral nutrition (homemade enteral preparations, blended enteral preparations, and commercial enteral formulas) for 3 days, using three hygiene procedures reported by caregivers: use of detergent (DET); use of detergent and boiling water (DET+BW); and use of detergent and bleach (DET+BL). The microbiological contamination was determined by the analysis of aerobic mesophilic microorganisms., Results: The bottles that were used for 3 days, regardless of the enteral nutrition category, were within the acceptable limit for aerobic mesophilic microorganisms (between <4 and 8.0 colony-forming units [CFU]/cm
2 ) when sanitized using the DET+BW and DET+BL procedures. The enteral nutrition bottles, when cleaned using the DET procedure during the 3 days of usage, showed low microbial contamination (between <4 and 3.0 CFU/cm2 ) in blended preparation and commercial formula only., Conclusion: Thus, regardless of the enteral nutrition category, we found that the bottles can be used for 3 days, as long as the DET+BW or DET+BL hygiene procedure is applied and safe food handling measures are adopted., (© 2024 American Society for Parenteral and Enteral Nutrition.)- Published
- 2024
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25. Postbiotics from Saccharomyces cerevisiae fermentation stabilize microbiota in rumen liquid digesta during grain-based subacute ruminal acidosis (SARA) in lactating dairy cows.
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Guo J, Zhang Z, Guan LL, Yoon I, Plaizier JC, and Khafipour E
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Background: Subacute ruminal acidosis (SARA) is a common metabolic disorder of high yielding dairy cows, and it is associated with dysbiosis of the rumen and gut microbiome and host inflammation. This study evaluated the impact of two postbiotics from Saccharomyces cerevisiae fermentation products (SCFP) on rumen liquid associated microbiota of lactating dairy cows subjected to repeated grain-based SARA challenges. A total of 32 rumen cannulated cows were randomly assigned to 4 treatments from 4 weeks before until 12 weeks after parturition. Treatment groups included a Control diet or diets supplemented with postbiotics (SCFPa, 14 g/d Original XPC; SCFPb-1X, 19 g/d NutriTek; SCFPb-2X, 38 g/d NutriTek, Diamond V, Cedar Rapids, IA, USA). Grain-based SARA challenges were conducted during week 5 (SARA1) and week 8 (SARA2) after parturition by replacing 20% DM of the base total mixed ration (TMR) with pellets containing 50% ground barley and 50% ground wheat. Total DNA from rumen liquid samples was subjected to V3-V4 16S rRNA gene amplicon sequencing. Characteristics of rumen microbiota were compared among treatments and SARA stages., Results: Both SARA challenges reduced the diversity and richness of rumen liquid microbiota, altered the overall composition (β-diversity), and its predicted functionality including carbohydrates and amino acids metabolic pathways. The SARA challenges also reduced the number of significant associations among different taxa, number of hub taxa and their composition in the microbial co-occurrence networks. Supplementation with SCFP postbiotics, in particular SCFPb-2X, enhanced the robustness of the rumen microbiota. The SCFP supplemented cows had less fluctuation in relative abundances of community members when exposed to SARA challenges. The SCFP supplementation promoted the populations of lactate utilizing and fibrolytic bacteria, including members of Ruminococcaceae and Lachnospiraceae, and also increased the numbers of hub taxa during non-SARA and SARA stages. Supplementation with SCFPb-2X prevented the fluctuations in the abundances of hub taxa that were positively correlated with the acetate concentration, and α- and β-diversity metrics in rumen liquid digesta., Conclusions: Induction of SARA challenges reduced microbiota richness and diversity and caused fluctuations in major bacterial phyla in rumen liquid microbiota in lactating dairy cows. Supplementation of SCFP postbiotics could attenuate adverse effects of SARA on rumen liquid microbiota., (© 2024. The Author(s).)
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- 2024
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26. Trajectory Patterns of Weight and Length in Children: From Birth Until 2-Years of Age.
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Dos Santos L, Cristino de Oliveira A, Marcondes Silva Lotz R, Manera F, Fernandes RC, Moreira Castilho B, and Höfelmann DA
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- Humans, Female, Male, Infant, Newborn, Infant, Brazil, Pregnancy, Child, Preschool, Cohort Studies, Body Weight, Weight Gain physiology, Child Development physiology, Birth Weight, Adult, Body Height physiology
- Abstract
Objective: The aim was to identify trajectory patterns of weight and length in children from birth until two years of life and establish associations with maternal and child characteristics., Methods: A mixed-cohort study was conducted in public health services in Colombo-PR, Brazil, between 2018 and 2022. Pregnancy information was gathered through anthropometric data collection and questionnaires. Birth data were extracted from birth record forms, while weight and length data in the first two years of life were obtained from physical and electronic health service records. Weight and length trajectory patterns were identified using a group-based trajectory model. The definition of the number of trajectory patterns to be selected considered the model fit to the type of variable, its practical utility, as well as the probabilities of group membership., Results: Two trajectory patterns of weight and length were identified among the children. The majority exhibited a pattern of weight (67.8%, n = 382) and length (90.9%, n = 472) considered high and stable, with a tendency to decelerate from one and a half years of age. The probability of belonging to the lower weight gain group was associated with female sex (41.5%, p < 0.001), smoking during pregnancy (48.7%, p = 0.008), prematurity (65.0%, p = 0.001), cesarean delivery (36.4%, p = 0.009), small for gestational age (69.0%, p < 0.001), and twinning (69.2%, p = 0.002). Similarly, the probability of belonging to the lower length gain group was associated with female sex (11.7%, p < 0.001), smoking during pregnancy (20.6%, p = 0.003), cesarean delivery (10.1%, p = 0.048), born small for gestational age (46.4%, p < 0.001), and twinning (46.1%, p < 0.001)., Conclusion: Conditions during pregnancy and childbirth can impact growth patterns in the first two years of life.
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- 2024
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27. Physicochemical and Sensory Properties and Antioxidant Activity of Xylitol Candies Containing Yuja ( Citrus junos ) Peels or Pulp.
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Im JH, Lee MK, and Lee HI
- Abstract
Xylitol candies offer numerous health benefits such as preventing cavities and obesity. However, a preference for them tends to be low due to their distinctive flavor. In this study, we developed xylitol candies containing mature yuja peel (MYP-C), immature yuja peel (IYP-C), and yuja pulp (YP-C). To determine the optimal yuja added to xylitol candy, we compared and analyzed its physicochemical properties, sensory characteristics, and antioxidant activities. IYP-C and MYP-C significantly increased the naringin and hesperidin contents compared to the control and the YP-C. In particular, the IYP-C exhibited the highest content of flavonoids and polyphenols, which contributed to enhancing antioxidant activity such as ferric reducing antioxidant power (FRAP), 1,1 diphenyl-2-picrylhydrazyl (DPPH), and 2,2'-azino-di-2 ethyl-benzothiazoline sulfonate (ABTS+) radical scavenging activities. The IYP-C had the highest crude ash content. The L*, a*, and b* values of MYP-C and IYP-C showed dark red and yellow colors compared to the CON and YP-C groups. The sensory analysis conducted using electronic tongue equipment revealed that IYP-C exhibited high levels of umami, sweetness, and bitterness, while YP-C showed the highest intensity of sourness. In conclusion, these results suggest that IYP-C rather than MYP-C and YP-C provide xylitol candy with good qualities in terms of antioxidant activities and physicochemical characteristics.
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- 2024
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28. Preparations offered to workers in different food services: application of the score for qualitative assessment of preparations.
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Dohms PODS, Stangarlin-Fiori L, and Medeiros CO
- Abstract
The quality of preparations offered in the workplace can vary according to the different segments of food services and may impact the health of the workers. This study aimed to qualitatively assess the food preparation offered to workers in from different food services. A total of 384 preparations were offered to workers in Curitiba City, Brazil. The preparations from three different segments of food services were evaluated: commercial (pilot study), non-commercial, and outsourced, selected for convenience. To identify the preparations, the nutritionist was interviewed, and the production process was monitored. The Score for Qualitative Assessment of Preparations (EAQP) was applied to evaluate the preparations, and they were classified according to their quality: high, intermediate, low, and very low quality. The chi-square and Kruskal-Wallis tests with post-hoc Least Significant Difference (LSD) Test were used. Most of the preparations were of high quality (72.9%), using mainly the unprocessed or minimally processed ingredients. The preparations offered by the non-commercial food service provider had a better mean quality score when compared to other food services ( p < 0.01). This study outcome is essential to help food service professionals to decide and choose the ingredients used in the preparations., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Dohms, Stangarlin-Fiori and Medeiros.)
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- 2024
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29. Systematic study on date palm seeds (Phoenix dactylifera L.) extraction optimisation using natural deep eutectic solvents and ultrasound technique.
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Alfaleh AA and Sindi HA
- Subjects
- Phenols analysis, Phenols chemistry, Chromatography, High Pressure Liquid methods, Polyphenols chemistry, Polyphenols analysis, Solvents chemistry, Choline chemistry, Ultrasonic Waves, Seeds chemistry, Phoeniceae chemistry, Plant Extracts chemistry, Deep Eutectic Solvents chemistry, Antioxidants chemistry
- Abstract
Natural deep eutectic solvents (NADES) are emerging, environment-friendly solvents that have garnered attention for their application in extracting phenolic compounds. This study investigated the effects of four synthetic NADES on polyphenols extracted from date seeds (DS) using choline chloride (ChCl) as a hydrogen-bond acceptor and lactic acid (La), citric acid (Citri), glycerol (Gly), and fructose (Fruc) as hydrogen-bond donors, in comparison with DS extracts extracted by conventional solvents (water, 70% methanol, and 70% ethanol). The antioxidant activity (DPPH), total phenolic content (TPC) and 6 phenolic compounds were determined using HPLC. The results showed that the ChCl-La and ChCl-Citri systems exhibited a high extraction efficiency regarding TPC, and DPPH in the DS extracts extracted by NADES compare to those DS extracts extracted with conventional solvents (p ˂ 0.001). HPLC results demonstrated that DS extracted by ChCl-La contained all measured phenolic compounds. Also gallic acid and catechin were the major compounds identified in the DS extracts. In addition DS extracted by ChCl-Citri and ChCl-Gly had the highest concentration of catechin. In conclusion, combining NADES is a promising and environment-friendly alternative to the conventional solvent extraction of phenolic compounds from DS., (© 2024. The Author(s).)
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- 2024
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30. Effect of 'Procumbenase' a serine protease from Tridax procumbens aqueous extract on wound healing: A scar free healing of full thickness wounds.
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Gubbiveeranna V, Megha GT, Kusuma CG, Ravikumar H, Thoyajakshi RS, Vijayakumar S, Mathad SN, Nagaraju S, Wazzan H, Khan A, Alzahrani KA, and Malash AM
- Subjects
- Animals, Mice, Rats, NIH 3T3 Cells, Male, Cicatrix drug therapy, Hydroxyproline metabolism, Tensile Strength, Wound Healing drug effects, Serine Proteases metabolism, Serine Proteases chemistry, Plant Extracts pharmacology, Plant Extracts chemistry
- Abstract
Wound healing involves several cellular and molecular pathways. Tridax procumbens activates genetic pathways with antibacterial, antioxidant, anticancer, and anti-inflammatory properties, aiding wound healing. This study purified Procumbenase, a serine protease from T. procumbens extract, using gel filtration (Sephadex G-75) and ion exchange (CM-Sephadex C-50) chromatography. Characterization involved analyses of protease activity, RP-HPLC, SDS-PAGE, gelatin zymogram, PAS staining, mass spectrometry, and circular dichroism. Optimal pH and temperature were determined. Protease type was identified using inhibitors. Wound-healing potential was evaluated through tensile strength, wound models, hydroxyproline estimation, and NIH 3T3 cell scratch analysis. In incision wound rat models, Procumbenase increased tensile strength on day 14 more than saline and Povidone‑iodine. It increased wound contraction by 89 % after 10 days in excision wound models, attaining full contraction by day 15 and closure by day 21. Scarless wound healing was enhanced by 18 days of epithelialization against 22 and 21 days for saline and povidone‑iodine. Procumbenase increased hydroxyproline concentration 2.53-fold (59.93 ± 2.89 mg/g) compared to saline (23.67 ± 1.86 mg/g). In NIH 3 T3 cell scratch assay, Procumbenase increased migration by 60.93 % (50 μg) and 60.57 % (150 μg) after 48 h. Thus, Procumbenase is the primary bioactive molecule in T. procumbens, demonstrates scar-free wound healing properties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2024
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31. Optimization of Enzyme-Assisted Extraction from Ginger ( Zingiber officinale ) Leaf and Its Immune-Stimulating Effects on Macrophages.
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Kim H, Kim M, Oh S, Hwang Y, and Choe JS
- Abstract
The ginger leaves contain terpenoids and phenolic compounds, such as gingerol and shogaol, which exert various physiological effects. This study focused on determining the optimal conditions for an enzyme (Ultimase MFC) extraction to enhance the bioactive components of underutilized ginger leaves using the response surface method. The extracted material was evaluated in terms of its yield and antioxidant capacity (total phenolic content, total flavonoid content, and activities of 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid). As a result, the optimal conditions included an enzyme concentration of 0.1% (v/v), a liquid-solid ratio of 33.939 mL/g, and an extraction time of 4 h. The optimized conditions resulted in an improvement in yield and antioxidant capacity, except for the total phenolic content of ginger leaves, when compared to the reference control extract. Additionally, the possibility of improving immunity was confirmed as nitric oxide and cytokines increased in macrophage cells compared with non-treatment control. Therefore, these extraction conditions enhance the potential industrial value of ginger leaves and underscore their promise as a natural ingredient for functional foods., Competing Interests: AUTHOR DISCLOSURE STATEMENT The authors declare no conflict of interest., (Copyright © 2024 by The Korean Society of Food Science and Nutrition.)
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- 2024
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32. Comparison of food intake pattern of diabetic patients and healthy individuals in a sample of Saudi population: a case-control study.
- Author
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Saaty AH and Aljadani HM
- Subjects
- Humans, Saudi Arabia, Male, Female, Case-Control Studies, Middle Aged, Adult, Diet statistics & numerical data, Aged, Exercise, Diabetes Mellitus, Type 2, Feeding Behavior
- Abstract
Background: There has been a significant rise in the number of individuals diagnosed with type 2 diabetes mellitus (T2DM), with the condition reaching epidemic proportions globally. This study examined the dietary pattern of a sample of Saudi Arabian adults with T2DM compared to control non-diabetics., Methods: Data from 414 participants, 207 control and 207 T2DM was analyzed. Anthropometric measurements, foods intake such as vegetables, fruits, whole grains, fried foods, sweetened juice, sweets, and pastries consumption as well as physical activity were obtained by an interview-survey., Results: The consumption of vegetables, green and leafy vegetables, starchy vegetables, fruits, proteins, and milk was significantly higher in the diabetics (p< 0.0001 for all and p<0.01 for starchy vegetables). Of the case group, 79.7% of them consumed whole-wheat bread while 54.6% of them consumed low fat milk (p<0.0001). There was a significant decrease in the percentage of cases who consumed discretionary foods and sweetened juices and soft drinks (24.1%), avoided sweets (75.8%) and pastries (37.1%), (p<0.0001). There were also significant increases in the percentages of participants who use healthy fat (as olive oil) in the case group (78.7%) (p<0.001). There was a significant increase in the percentage of diabetics who followed a diet to lose weight (15%) (p<0.05). The majority of the two study groups were physically inactive (control 95.2% & case 94.2%)., Conclusions: The results of this study provide insight on that diabetics generally follow a healthy diet, yet their engagement in physical activity may not be optimal., (© 2024. The Author(s).)
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- 2024
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33. Nutritional composition of ultra-processed plant-based foods in the out-of-home environment: a multi-country survey with plant-based burgers.
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Vellinga RE, Rippin HL, Gonzales BG, Temme EHM, Farrand C, Halloran A, Clough B, Wickramasinghe K, Santos M, Fontes T, Pires MJ, Nascimento AC, Santiago S, Burt HE, Brown MK, Jenner KH, Alessandrini R, Marczak AM, Flore R, Sun Y, and Motta C
- Subjects
- Humans, Amino Acids analysis, Dietary Proteins analysis, Nutrients analysis, Food Handling methods, Minerals analysis, Dietary Fats analysis, Dietary Carbohydrates analysis, Fast Foods analysis, Bread analysis, Nutritive Value, Dietary Fiber analysis, Energy Intake
- Abstract
Ultra-processed plant-based foods, such as plant-based burgers, have gained in popularity. Particularly in the out-of-home (OOH) environment, evidence regarding their nutritional profile and environmental sustainability is still evolving. Plant-based burgers available at selected OOH sites were randomly sampled in Amsterdam, Copenhagen, Lisbon and London. Plant-based burgers (patty, bread and condiment) ( n 41) were lab analysed for their energy, macronutrients, amino acids and minerals content per 100 g and serving and were compared with reference values. For the plant-based burgers, the median values per 100 g were 234 kcal, 20·8 g carbohydrates, 3·5 g dietary fibre and 12·0 g fat, including 0·08 g TFS and 2·2 g SFA. Protein content was 8·9 g/100 g, with low protein quality according to amino acid composition. Median Na content was 389 mg/100 g, equivalent to 1 g salt. Compared with references, the median serving provided 31% of energy intake based on a 2000 kcal per day and contributed to carbohydrates (17-28%), dietary fibre (42%), protein (40%), total fat (48%), SFA (26%) and Na (54%). One serving provided 15-23% of the reference values for Ca, K and Mg, while higher contributions were found for Zn, Mn, P and Fe (30-67%). The ultra-processed plant-based burgers provide protein, dietary fibre and essential minerals and contain relatively high levels of energy, Na and total fats. The amino acid composition indicated low protein quality. The multifaceted nutritional profile of plant-based burgers highlights the need for manufacturers to implement improvements to better support healthy dietary habits, including reducing energy, Na and total fats.
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- 2024
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34. Listeria monocytogenes from Food Products and Food Associated Environments: Antimicrobial Resistance, Genetic Clustering and Biofilm Insights.
- Author
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Silva A, Silva V, Gomes JP, Coelho A, Batista R, Saraiva C, Esteves A, Martins Â, Contente D, Diaz-Formoso L, Cintas LM, Igrejas G, Borges V, and Poeta P
- Abstract
Listeria monocytogenes , a foodborne pathogen, exhibits high adaptability to adverse environmental conditions and is common in the food industry, especially in ready-to-eat foods. L. monocytogenes strains pose food safety challenges due to their ability to form biofilms, increased resistance to disinfectants, and long-term persistence in the environment. The aim of this study was to evaluate the presence and genetic diversity of L. monocytogenes in food and related environmental products collected from 2014 to 2022 and assess antibiotic susceptibility and biofilm formation abilities. L. monocytogenes was identified in 13 out of the 227 (6%) of samples, 7 from food products (meat preparation, cheeses, and raw milk) and 6 from food-processing environments (slaughterhouse-floor and catering establishments). All isolates exhibited high biofilm-forming capacity and antibiotic susceptibility testing showed resistance to several classes of antibiotics, especially trimethoprim-sulfamethoxazole and erythromycin. Genotyping and core-genome clustering identified eight sequence types and a cluster of three very closely related ST3 isolates (all from food), suggesting a common contamination source. Whole-genome sequencing (WGS) analysis revealed resistance genes conferring resistance to fosfomycin ( fosX ), lincosamides ( lin ), fluoroquinolones ( norB ), and tetracycline ( tetM ). In addition, the qacJ gene was also detected, conferring resistance to disinfecting agents and antiseptics. Virulence gene profiling revealed the presence of 92 associated genes associated with pathogenicity, adherence, and persistence. These findings underscore the presence of L. monocytogenes strains in food products and food-associated environments, demonstrating a high virulence of these strains associated with resistance genes to antibiotics, but also to disinfectants and antiseptics. Moreover, they emphasize the need for continuous surveillance, effective risk assessment, and rigorous control measures to minimize the public health risks associated to severe infections, particularly listeriosis outbreaks. A better understanding of the complex dynamics of pathogens in food products and their associated environments can help improve overall food safety and develop more effective strategies to prevent severe health consequences and economic losses.
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- 2024
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35. Mackerel and Seaweed Burger as a Functional Product for Brain and Cognitive Aging Prevention.
- Author
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Cardoso C, Valentim J, Gomes R, Matos J, Rego A, Coelho I, Delgado I, Motta C, Castanheira I, Prates JAM, Bandarra NM, and Afonso C
- Abstract
Most world countries are experiencing a remarkable aging process. Meanwhile, 50 million people are affected by Alzheimer's disease (AD) and related dementia and there is an increasing trend in the incidence of these major health problems. In order to address these, the increasing evidence suggesting the protective effect of dietary interventions against cognitive decline during aging may suggest a response to this challenge. There are nutrients with a neuroprotective effect. However, Western diets are poor in healthy n-3 polyunsaturated fatty acids (n-3 PUFAs), such as docosahexaenoic acid (DHA), iodine (I), and other nutrients that may protect against cognitive aging. Given DHA richness in chub mackerel ( Scomber colias ), high vitamin B9 levels in quinoa ( Chenopodium quinoa ), and I abundance in the seaweed Saccorhiza polyschides , a functional hamburger rich in these nutrients by using these ingredients was developed and its formulation was optimized in preliminary testing. The effects of culinary treatment (steaming, roasting, and grilling vs. raw) and digestion on bioaccessibility were evaluated. The hamburgers had high levels of n-3 PUFAs in the range of 42.0-46.4% and low levels of n-6 PUFAs (6.6-6.9%), resulting in high n-3/n-6 ratios (>6). Bioaccessibility studies showed that the hamburgers could provide the daily requirements of eicosapentaenoic acid (EPA) + DHA with 19.6 g raw, 18.6 g steamed, 18.9 g roasted, or 15.1 g grilled hamburgers. Polyphenol enrichment by the seaweed and antioxidant activity were limited. The hamburgers contained high levels of Se and I at 48-61 μg/100 g ww and 221-255 μg/100 g ww, respectively. Selenium (Se) and I bioaccessibility levels were 70-85% and 57-70%, respectively, which can be considered high levels. Nonetheless, for reaching dietary requirements, considering the influence of culinary treatment and bioaccessibility, 152.2-184.2 g would be necessary to ensure daily Se requirements and 92.0-118.1 g for I needs.
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- 2024
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36. Antiproliferative and Apoptotic Effects of Murici (Byrsonima crassifolia (L.) Kunth and verbascifolia (L.) DC) and Taperebá (Spondias mombin L.) Extracts in Human Prostate Cell Line (PC-3).
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Amaral Muxfeldt Paim TOD, Simas NF, Abreu JP, Rosa L, Passos Barbosa Lima T, and Teodoro AJ
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- Humans, Male, Cell Cycle drug effects, Tumor Cells, Cultured, PC-3 Cells, Cell Survival drug effects, Apoptosis drug effects, Cell Proliferation drug effects, Plant Extracts pharmacology, Prostatic Neoplasms drug therapy, Prostatic Neoplasms pathology
- Abstract
Objective: The present study aimed to evaluate the antiproliferative and apoptotic effects of extracts obtained from the murici (Byrsonima crassifolia (L.) Kunth and verbascifolia (L.) DC) and taperebá (Spondias mombin L.) pulps, on cell proliferation, cell cycle and apoptosis on human prostate cell line (PC-3)., Methods: Four extract was produced from the pulps: murici aqueous extract (MA), taperebá aqueous extract (TA), murici ethanolic extract (ME) and taperebá ethanolic extract (TE). In the present study, the analysis of cell viability, cell cycle and apoptosis analyze were performed using the MTT method and flow cytometry., Results: The results showed that murici and taperebá extracts proved to be inhibitors of cell growth, modulation of cell cycle promoters and capable of enhancing the death in prostate carcinoma cells PC-3; suggesting a regulatory effect in prostate cell line, depending on type of extract and dosage used., Conclusion: These results open a series of perspectives on the use of these bioactive extracts in the prevention and treatment of prostate cancer.
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- 2024
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37. The first harmonised total diet study in Portugal: Vitamin D occurrence and intake assessment.
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Graça Dias M, Vasco E, Ravasco F, and Oliveira L
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- Male, Female, Animals, Portugal, Vitamins, Dietary Supplements, Vitamin D, Diet
- Abstract
Vitamin D acts in calcium and phosphate homeostasis and also as an immunomodulatory hormone. To estimate the vitamin D intake by the 'adults' and 'elderly' Portuguese populations TDS methodology was used, since in the absence of skin UVB exposure, food and supplements are the only vitamin D sources. Vitamin D was quantifiable in 78 (24 from the fish group) of the 164 TDS samples. Sea bream contained the most vitamin D (13.8 µg/100 g), followed by plaice (9.2 µg/100 g). MCRA software (semi-probabilistic approach) was used to estimate the median vitamin D intake that ranged between 2.47 ('adults' 'males') - 1.45 ('elderly' 'females') µg/day, well below the Dietary Reference Values (5-15 µg/day). Plaice, sea bream and sardine were the main contributors to intake. A prevalence of 94% inadequate vitamin D intake for 'adults' and 'elderly' was found based on the estimated average requirement of 10 µg/day., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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38. Assessment of Broad-Spectrum Antimicrobial, Antibiofilm, and Anticancer Potential of Lactoferrin Extracted from Camel Milk.
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Alkhulaifi MM, Alosaimi MM, Khan MS, Tabrez S, Shaik GM, Alokail MS, Hassan MA, Awadalla ME, and Husain FM
- Subjects
- Animals, Humans, Lactoferrin pharmacology, Lactoferrin chemistry, Staphylococcus aureus, Camelus, Milk chemistry, Microbial Sensitivity Tests, Bacteria, Biofilms, Anti-Bacterial Agents chemistry, Methicillin-Resistant Staphylococcus aureus, Anti-Infective Agents pharmacology
- Abstract
Lactoferrin is a multifunctional glycoprotein present in mammalian milk. It possesses antimicrobial, antioxidant, immunomodulatory, and several biological functions. Owing to the current trend of increasing antibiotic resistance, our study was designed to purify lactoferrin from camel milk colostrum using cation exchange chromatography on the SP-Sepharose high-performance column. The purity and molecular weight of lactoferrin were checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The chromatogram of the purification procedure illustrated a single peak corresponding to lactoferrin, while the SDS-PAGE revealed 78 kDa molecular weight protein. Furthermore, lactoferrin protein and its hydrolysate form were assessed for its antimicrobial potential. The highest inhibitory effect of whole lactoferrin at the concentration (4 mg/ml) was observed against methicillin-resistant S. aureus (MRSA) and S. aureus, while 10 mg/ml concentration was effective against K. pneumonia, and 27 mg/ml was potent against multidrug-resistant (MDR) bacteria, P. aeruginosa. Likewise, MRSA was more sensitive toward iron-free lactoferrin (2 mg/ml) and hydrolyzed lactoferrin (6 mg/ml). The tested lactoferrin forms showed variability in minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) among tested bacteria. The scanning electron microscopy (SEM) analysis images revealed distortions of the bacterial cells exposed to lactoferrin. The antibiofilm effect differed depending on the concentration and the type of the bacteria; biofilm inhibition ranged from 12.5 to 91.3% in the tested pathogenic bacteria. Moreover, the anticancer activity of lactoferrin forms exhibited a dose-dependent cytotoxicity against human lung cancer cell line (A549)., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2024
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39. Simultaneity of health-related behaviors and food insecurity among pregnant women.
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Fernandes RC and Höfelmann DA
- Abstract
The researchers aimed through this cross-sectional study to explore the co-occurrence of health-related behaviors and their association with food insecurity in pregnant women. The investigated behaviors include smoking, alcohol consumption, non-regular consumption of fruit, and physical inactivity among 605 participants. Our findings reveal that women experiencing food insecurity exhibit a higher prevalence of simultaneous engagement in specific health behaviors. Specifically, the co-occurrence of alcohol consumption and non-regular fruit consumption is elevated, as is the co-occurrence of smoking, alcohol consumption, and physical inactivity, and alcohol consumption, regular fruit consumption, and physical inactivity. The implications of these findings underscore the need for targeted interventions addressing both food insecurity and clustered health behaviors during pregnancy.
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- 2024
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40. The Potential Effects of Dietary Antioxidants in Obesity: A Comprehensive Review of the Literature.
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Almoraie NM and Shatwan IM
- Abstract
Obesity has become a global health concern, with its prevalence steadily increasing in recent decades. It is associated with numerous health complications, including cardiovascular diseases, diabetes, and certain types of cancer. The aetiology of obesity is multifactorial, involving genetic, environmental, and lifestyle factors. In recent years, oxidative stress has emerged as a potential contributor to obesity and its related metabolic disorders. Dietary antioxidants, which can counteract oxidative stress, have gained significant attention for their potential role in preventing and managing obesity. This comprehensive review aims to explore the impact of dietary antioxidants on obesity and its associated metabolic dysregulations, discussing the underlying mechanisms and highlighting the potential therapeutic implications.
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- 2024
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41. Consumption of ultra-processed foods and risk for Alzheimer's disease: a systematic review.
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Claudino PA, Bueno NB, Piloneto S, Halaiko D, Azevedo de Sousa LP, Barroso Jara Maia CH, and Netto BDM
- Abstract
Objective: To investigate the association of the consumption of ultra-processed foods with the risk of developing Alzheimer's disease in adults and the elderly. The review protocol was registered on PROSPERO (CRD42022375944)., Methods: This is a systematic review reported according to PRISMA guidelines. Observational studies were included without language or publication year restrictions. Studies assessing only other types of dementia as outcomes, not considering Alzheimer's disease, were excluded. The research was carried out in the Medline, Embase, Lilacs databases, and a survey of the gray literature between April and November 2023, in addition to citation search in the included studies. Data extraction was performed by two independent reviewers. The risk of bias and methodological quality of the included studies were assessed using the Joanna Briggs Institute checklist for cohort studies., Results: A total of 5 studies involving 617,502 adults and elderly people were included. All studies had a cohort design and were considered of high methodological quality. Of the included studies, 4 demonstrated a risk association between the consumption of ultra-processed foods and the development of Alzheimer's disease, while 1 study showed a risk association only with the development of cognitive decline., Discussion: The association between ultra-processed foods consumption and the risk of developing Alzheimer's disease is a recent topic in scientific studies, given that the oldest study identified by our review dates back to 2017. Of the four included studies, three showed a significant association between ultra-processed foods consumption and the risk of developing Alzheimer's disease., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Claudino, Bueno, Piloneto, Halaiko, Azevedo de Sousa, Barroso Jara Maia and Netto.)
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- 2024
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42. A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transepithelial Transport of Food Compounds.
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Hevia A, Ruas-Madiedo P, Faria MA, Petit V, Alves B, Alvito P, Arranz E, Bastiaan-Net S, Corredig M, Dijk W, Dupont D, Giblin L, Graf BA, Kondrashina A, Ramos H, Ruiz L, Santos-Hernández M, Soriano-Romaní L, Tomás-Cobos L, Vivanco-Maroto SM, Recio I, and Miralles B
- Subjects
- Humans, Biological Transport, Intestinal Absorption, Caco-2 Cells, Intestines, Food
- Abstract
Assessing nutrient bioavailability is complex, as the process involves multiple digestion steps, several cellular environments, and regulatory-metabolic mechanisms. Several in vitro models of different physiological relevance are used to study nutrient absorption, providing significant challenges in data evaluation. However, such in vitro models are needed for mechanistic studies as well as to screen for biological functionality of the food structures designed. This collaborative work aims to put into perspective the wide-range of models to assay the permeability of food compounds considering the particular nature of the different molecules, and, where possible, in vivo data are provided for comparison.
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- 2023
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43. Gender and the length of time since autologous hematopoietic stem cell transplantation-What is their influence on the immune reconstitution in multiple myeloma patients?
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Popierz-Rydlewska N, Merkiel-Pawłowska S, Łojko-Dankowska A, Komarnicki M, and Chalcarz W
- Subjects
- Humans, Male, Female, Transplantation, Autologous, Immune System, Multiple Myeloma therapy, Immune Reconstitution, Hematopoietic Stem Cell Transplantation methods
- Abstract
Introduction: In the literature there is lack of information on the influence of gender and time since autologous hematopoietic stem cell transplantation (HSCT) on the immune reconstitution in multiple myeloma (MM) patients., Objective: The aim of this study was to assess the diversity of the immune reconstitution according to gender in MM patients after autologous HSCT on the day of the clinic discharge and on the 29th day after discharge, as well as to investigate the changes in the immune system in females and males after staying at home for 28 days., Method: The studied population comprised 13 females and 13 males after autologous HSCT. On the day of the clinic discharge and on the 29th day after discharge blood samples were taken to analyse 22 immunological parameters. Statistical analysis was performed using STATISTICA 10 StatSoft Poland. For multiple comparisons, the Bonferroni correction was used., Results: No statistically significant differences were observed in the analysed immunological parameters between the studied females and males with MM on the day of the clinic discharge and on the 29th day after discharge. However, on the 29th day after the clinic discharge compared to the day of the clinic discharge, statistically significant differences were found in 8 immunological parameters among females and 6 immunological parameters among males., Conclusion and Recommendation: Our results indicate that the immune reconstitution is similar but not the same in patients of both genders. Statistically significant differences in the immune response in the studied females and males imply that gender may play a role in the immune reconstitution and that the results obtained in MM patients should be analysed separately in females and males. In order to explain the observed changes in the immune system according to gender, further research should be carried out on a larger population. This would most probably make it possible to find their clinical application., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Popierz-Rydlewska et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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- 2023
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44. Checklist to assess food safety when handling home enteral nutrition.
- Author
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da Costa RL, Medeiros CO, da Cunha DT, and Stangarlin-Fiori L
- Subjects
- Humans, Food Safety, Health Personnel, Enteral Nutrition, Checklist
- Abstract
Background: Identifying food safety risks when handling enteral formulations at home is important to restore and maintain the health of patients. Therefore, this study developed and validated a food safety assessment checklist for handling home enteral nutrition (HEN)., Methods: This methodological study developed a checklist based on a literature review and interviews with food safety professionals. The content validation, which was conducted by food safety and enteral feeding experts, assessed the relevance, clarity, and simplicity of the checklist using the content validity index of items (CVI-I), categories (CVI-C), and the entire instrument (CVI-EI). Each item was rated as either essential, necessary, or recommended according to the risk in relation to foodborne diseases., Results: The Food Safety for Handling Home Enteral Nutrition checklist (FSHEN checklist) that was developed includes 40 items and the following eight categories: physical structure; cleaning of facilities, equipment, furniture, and utensils; pests and waste; water supply; food handlers; ingredients and packaging; handling of enteral formulations; and the bottling and storage of enteral formulations. The CVI-EI was above the recommendation (≥0.93) for the three assessed criteria, as was the CVI-C in terms of clarity (≥0.95), relevance (≥0.98), and simplicity (≥0.84). A total of 52.5% of the items were rated as essential, 32.5% as necessary, and 15% as recommended., Conclusion: The FSHEN checklist is relevant, clear, and easy to use. It can help health professionals and individuals working in the field to assess and prioritize items to improve food safety in the management of HEN., (© 2023 American Society for Parenteral and Enteral Nutrition.)
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- 2023
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45. Adherence to the Mediterranean diet is associated with a reduced DAS28 index among patients with rheumatoid arthritis: Case study from KRRD.
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Alawadhi B, Alsaber A, Shatawan I, Al-Herz A, Setiya P, Saleh K, Al-Awadhi A, Hasan E, Al-Kandari W, Mokaddem K, Ghanem A, Attia Y, Hussain M, Alhadhood N, Ali Y, Tarakmeh H, Aldabie G, AlKadi A, Alhajeri H, and Aldei A
- Subjects
- Adult, Humans, Cross-Sectional Studies, Joints, Severity of Illness Index, Diet, Mediterranean, Arthritis, Rheumatoid diagnosis, Arthritis, Rheumatoid drug therapy, Arthritis, Rheumatoid epidemiology, Biological Products therapeutic use
- Abstract
Background: Rheumatoid arthritis (RA) is a chronic, autoimmune disease that mostly affects the synovial joints. It has been hypothesized that dietary and other environmental and lifestyle factors contribute to the development of RA and its severity., Objective: The present study aims to measure the effect of the Mediterranean diet (MedDiet) on the disease activity scores (DAS28) among patients with RA., Methods: Adult patients who satisfied the American College of Rheumatology (ACR) classification criteria for RA from major hospitals in Kuwait were evaluated. A cross-sectional study conducted on 754 RA patients visits aged (21-79) years. Patients were evaluated using the DAS28. Patients' levels of adherence to the MedDiet are assessed using a validated 14-item Questionnaire (paper or web-based). The data was analyzed using both multivariate and univariate statistics. Multivariate logistic regression was used to analyze the statistical relationship between MedDiet and RA disease activity., Results: The finding suggests that a MedDiet can have a positive impact on DAS28 among patients with RA. In the DAS28 cohort (DAS28 < 3.2, DAS28 ≥ 3.2), several Mediterranean survey components showed statistically significant differences. Patients with a Mediterranean score ≤ 5 was more likely to have hazard effects for DAS28 than those with a Mediterranean score of ≥10 (HR = 0.17, CI [0.08-0.37], p < .001). The finding shows that, Mediterranean levels ≤5, on biologics treatment, CRP, and patient global assessment were significantly associated with overall survival. Additionally, the MedDiet was found to be a significant predictor of DAS28 in the random forest decision tree plot, along with tender, RF, and creatinine. MedDiet patients had a lower DAS28 score than others., Conclusion: The findings suggest that optimal drug treatment and a restrictive diet can help to improve DAS28 score for patients with RA. More patients in the cohort DAS28 <3.2 used olive oil, servings of vegetables, fruits, and legumes. In contrast, more patients in the cohort DAS28 ≥ 3.2 consumed red meat, butter, sweetened or soft drinks, cakes, cookies, or biscuits, and tomato sauce., (© 2023 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons Australia, Ltd.)
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- 2023
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46. Acute repeated cage exchange stress modifies urinary stress and plasma metabolic profiles in male mice.
- Author
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Fushuku S, Ushikai M, Arimura E, Komaki Y, and Horiuchi M
- Subjects
- Humans, Mice, Animals, Male, Amino Acids, Glucose, Catecholamines, Stress, Psychological complications, Corticosterone, Restraint, Physical
- Abstract
Exposure to a novel environment is psychologically and physically stressful for humans and animals. The response has been reported to involve enhanced sympathetic nervous system activity, but changes in nutrient levels under stress are not fully understood. As a form of exposure to a novel environment, repeated cage exchange (CE, four times at 2-h intervals for 8 h from 08:00 h) during the light phase with no restraint on movement was applied to A/J mice, a strain particularly prone to stress. Body temperature was measured with a temperature-sensing microchip implanted in the interscapular region. The stress conditions and anxiety level were evaluated by measuring urinary catecholamines and corticosterone and by performing an anxiety-like behavior test, respectively. Major nutrients such as glucose, fatty acids, and amino acids in the plasma were also examined. CE mice showed a significant increase in body temperature with each CE. They also showed a significantly greater reduction of body weight change, more water intake, and higher levels of urinary catecholamines and corticosterone and anxiety-like behavior score than control mice. The model revealed a significantly lower plasma glucose level and higher levels of several essential amino acids, such as branched-chain amino acids and phenylalanine, than those of control mice. Meanwhile, free fatty acids and several amino acids such as arginine, aspartic acid, proline, threonine, and tryptophan in both sets of mice were significantly decreased from the corresponding levels at 08:00 h, while similar plasma levels were exhibited between mice with and without CE. In conclusion, repeated CE stress was associated with changes in glucose and amino acids in plasma. Although further study is needed to clarify how these changes are specifically linked to anxiety-like behavior, this study suggests the potential for nutritional intervention to counter stress in humans exposed to novel environments., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Fushuku et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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- 2023
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47. Lipid profile and cost of enteral nutrition formula with addition of fish oil used in a public hospital.
- Author
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Castanheira Neia VJ, Landi Masquio DC, Claudino PA, Duso P, Tadano DK, Bolognese MA, Magalhães de Souza P, Santos OO, Visentainer JV, and Molin Netto BD
- Subjects
- Humans, Enteral Nutrition, Eicosapentaenoic Acid, Fatty Acids, Docosahexaenoic Acids, Hospitals, Public, Fish Oils, Fatty Acids, Omega-3
- Abstract
Background & Aims: The present study evaluated the lipid profile of enteral nutrition formulas with added fish oil used in a public hospital, with an emphasis on the fatty acid (FA) composition., Methods: FA composition was determined using gas chromatography coupled to a flame ionization detector (GC-FID). Nine enteral nutrition formulas were evaluated and the results obtained were compared with those reported on the formulas labels., Results: The sample with the highest percentage of added fish oil according to the label information had the lowest total amounts of eicosapentaenoic acid and docosahexaenoic acid (EPA + DHA) (p < 0.05). In the evaluation of the total amount of polyunsaturated fatty acids/saturated fatty acids (ΣPUFA/ΣSFA) ratio, five samples were not within the values recommended by Brazilan and international health regulatory agencies. Regarding the n-6/n-3 fatty acids ratio, five samples showed values higher than the recommended ratio. It was observed that EPA + DHA content was positively correlated with the cost of the diet. Importantly, we also found that there was a significant difference between the results of our analysis and the descriptions found on the labels for fatty acids n-6, n-3, EPA, DHA, SFA, PUFA and MUFA., Conclusions: We conclude that the discrepancies for fatty acids between the values obtained in the analyses and the values reported on the labels highlight the need for more rigorous inspection when public hospitals purchase enteral nutrition formulas with added fish oil, since the administration of formulas with inadequate levels of FA in hospitalized patients can compromise clinical results during the hospitalization period., (Copyright © 2023. Published by Elsevier Ltd.)
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- 2023
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48. The consumption of dietary supplements in Saudi Arabia during the COVID-19 pandemic: A cross-sectional study.
- Author
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Azhar W, Al-Otaibi K, Abusudah WF, Azzeh F, Qhadi A, Alhassani WE, Almohmadi NH, Bushnaq T, Tashkandi B, Alharbi NA, Babteen A, Ghabashi M, Kamfar Y, and Ghafouri K
- Abstract
Background and Objectives: People frequently utilize dietary supplements (DS), notably during the COVID-19 epidemic, despite the lack of data supporting their usefulness and safety in enhancing general health. This study aimed to measure the consumption of DS in Saudi Arabia during COVID-19. Previous studies focused on using dietary supplements for preventing or reducing mental health., Materials and Methods: A cross-sectional study was conducted with 1572 participants aged 18-65, from all-over Saudi Arabia who were invited to complete a self-administered questionnaire to measure the consumption of supplements during the pandemic lockdown period. Also, it investigates the factor that effect supplements consumption., Results: Around 63% of the participants did not change their consumption patterns during the pandemic, while 16% consumed more and 21.4% consumed fewer supplements during the pandemic. The most commonly used supplements were iron; calcium, especially among pregnant women; omega-3, especially in people older than 65 years; and vitamin D. Females (81%; P < 0.002); age (94.7%; P < 0.002); married people (84%; P < 0.001); those with a higher educational level (83.9%; P = 0.02); those with a higher monthly income (86.1%; P = 0.006); and pregnant women (100%; P < 0.007) reported the highest rates of consumption., Conclusion: Dietary supplements have recently become popular in Saudi Arabia, but large differences remain between Saudis in their consumption of supplements. Additional research should be conducted to examine the level of knowledge of dietary supplements among Saudi population., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Author(s).)
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- 2023
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49. Young adults' sought gratifications from, and perceptions of food advertising by, social media influencers: a qualitative approach.
- Author
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Alhothali GT and Aljefree NM
- Subjects
- Humans, Young Adult, Pleasure, Food, Marketing, Advertising, Social Media
- Abstract
Background: This study aims to explore young adults' sought gratifications from social media influencers and whether they are exposed to food advertising by influencers. Further, it aims to understand how young individuals perceive food advertisements by social media influencers., Methods: This qualitative study was conducted on 17 students from two public universities-King Abdul Aziz and Jeddah universities-located in the City of Jeddah, Saudi Arabia, using in-depth, face-to-face, and semi-structured interviews. The participants were active users of social media platforms who followed at least one influencer. Thematic analysis was used to analyse the data. The COREQ guidelines for reporting qualitative research (see Additional file 2) were followed when stating the findings., Results: The findings reveal ten themes, that is, seven gratifications sought by young adults-broadening knowledge, perceived usefulness, self-improvement, boosting positivity, fostering morale, reinforcing inspiration, and passing time/enjoyment-and three themes (i.e. repeated, authentic, and unhealthy) that describe how Saudi young adults perceive food advertising., Conclusion: This study contributes to social media influencer marketing by identifying the factors that motivate young consumers to follow influencers, and it elucidates the extent to which young adults are exposed to food marketing, adding to the body of literature on food advertising., (© 2023. BioMed Central Ltd., part of Springer Nature.)
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- 2023
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50. Capacity needs assessment and challenges for multisectoral implementation of nutrition in Burkina Faso: A guide for the formulation of a capacity development plan.
- Author
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Diasso D, Halidou Doudou M, Cruz S, Tonnoir F, Compaoré-Sérémé D, Zongo U, and Savadogo A
- Subjects
- Humans, Burkina Faso, Needs Assessment, Social Planning, Nutritional Status, United Nations
- Abstract
Introduction: Achieving nutritional goals depends on individual, organisational and environmental capacities. The aim of this study was to analyse and identify capacity gaps among the coordination platforms and networks, and the key technical institutions related to nutrition in Burkina Faso for a capacity development plan formulation., Methods: Using the new Nutrition Capacity Framework developed by the United Nations Network, information were collected using the Nutrition Stakeholder Mapping and Analysis tool, and the Checklist for Capacity Areas. Capacity needs were analysed in terms of Human resource and infrastructure, functional, organisational, coordination and partnership, and financial and resource mobilisation., Results: Limited human resource capacity in nutrition was highlighted in most cases by the structures, and the nutrition coordination structure and more than 4/5 of the technical structures are faced with the unavailability of working materials, tools and basic Internet connection. Only 10 among the 30 structures have a unit or service for exchange on nutrition, and only three of them have integrated nutrition actions. Shortfalls were noted in terms of functional, facilitation, communication and advocacy skills, as well as a weak diversification of resource mobilisation strategies., Conclusion: The use of the analytical framework helped to identify the gaps and to propose paths for capacity development. Efforts need to be strengthened, intensified, coordinated, monitored, evaluated and funded., (© 2023 John Wiley & Sons Ltd.)
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- 2023
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