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Purpose: To assess the ability to control the steering of a modified guidewire actuated by the magnetic force of a magnetic resonance imaging system with additional gradient coils for selective arterial catheterization in rabbits. Methods: Selective catheterizations of the right renal artery, left renal artery, superior mesenteric artery, and iliac artery were performed on two rabbits. A 3D magnetic force was applied onto a magnetic bead placed at the tip of a guidewire. The ability of the guidewire to advance in the aorta without entering the side branches when the magnetic force was not applied was also evaluated. Steering of the guidewire was combined with a dedicated tracking system and its position was registered on the 3D model of a magnetic resonance angiography (MRA). Results: The magnetic catheterization of the renal arteries was successful and showed reproducibility. Superior mesenteric artery and iliac artery showed that the catheterization was feasible. These two arteries were difficult to visualize on MRA, making catheterization and setting the direction of the force more difficult. There was no inadvertent catheterization of side vessels when the guidewire was advanced with magnetic steering despite the hook shape at the tip of the guidewire caused by the alignment of the bead anisotropy with the permanent magnetic field. Conclusions: This first evaluation of selective catheterization of aortic branches with a magnetic guidewire provided a successful steering in the less angled side branches and this modified guidewire was advanced in the aorta without inadvertent selective catheterization when manipulated without magnetic actuation.

We hypothesized that a relationship existed between the mechanical properties and the magnetic resonance imaging (MRI) parameters of muscles, as already demonstrated in cartilaginous tissues. The aim was to develop an indirect evaluation tool of the mechanical properties of degenerated muscles. Leg and arm muscles of adult rabbits were dissected, and tested 12 hours post mortem, in a state of rigor mortis, or 72 hours post mortem, in a state of post-rigor mortis. The tests consisted of a multi-parametric MRI acquisition followed by a uniaxial tensile test until failure. The statistical analysis consisted of multiple linear regressions and principal component analysis. Significant differences existed between the rigor mortis and post-rigor mortis groups for E but not for the MRI parameters. 78%, 60% or 33% of the Young’s modulus could be explained by the MRI parameters in the post-rigor mortis group, rigor mortis group or both groups respectively. These relationships were confirmed by the principal component analysis. The proposed multi-parametric MRI protocol associated to principal component analysis is a promising tool for the indirect evaluation of muscle mechanical properties and should be useful to find biomarkers and predictive factors of the evolution of the pathologies.

Magnetic resonance navigation (MRN), achieved with an upgraded MRI scanner, aims to guide new therapeutic magnetic microcarriers (TMMC) from their release in the hepatic vascular network to liver tumor. In this technical note, in vitro and in vivo MRI properties of TMMC, loaded with iron–cobalt nanoparticles and doxorubicin, are reported by following three objectives: (1) to evaluate the lengthening of echo-time (TE) on nano/microparticle imaging; (2) to characterize by MRI TMMC distribution in the liver; and (3) to confirm the feasibility of monitoring particle distribution in real time. Phantom studies were conducted to analyze nano/microparticle signals on T 2*-weighted gradient-echo (GRE) MR images according to sample weight and TE. Twelve animal experiments were used to determine in vivo MRI parameters. TMMC tracking was evaluated by magnetic resonance imaging (MRI) in four rabbits, which underwent MRN in the hepatic artery, three without steering, two in real-time, and three as blank controls. TMMC distribution in the right and left liver lobes, determined by ex vivo MR image analysis, was compared to the one obtained by cobalt level analysis. TMMC induced a hypointense signal that overran the physical size of the sample on MR images. This signal, due to the nanoparticles embedded into the microparticles, increased significantly with echo-time and sample amount (p

Objective Magnetic resonance imaging (MRI) offers great potential as a sensitive and noninvasive technique for describing the alterations in mechanical properties, as shown in vitro on intervertebral disc (IVD) or cartilage tissues. However, in vivo, the IVD is submitted to complex loading stimuli. Thus, the present question focuses on the influence of the mechanical loading during an MRI acquisition on the relaxation times, magnetization transfer and diffusion parameters within the IVD. Methods An apparatus allowing the compression of isolated IVDs was designed and manufactured in acrylonitrile butadiene styrene. IVDs were dissected from fresh young bovine tail, measured for their thickness and submitted to compression just before the MRI acquisition. Six discs received 0% (platen positioned at the initial disc thickness), 5% (platen positioned at 95% of the initial disc thickness), 10%, 20% and 40% deformation. The MRI parameters were compared between the loading states using mean and standard deviation for T1 and T2, and matrix subtraction for Magnetization Transfer, fractional anisotropy and apparent diffusion coefficient. Results The compression of the IVD did not lead to any significant change of the MRI parameters, except for the diffusion that decreased in the direction of the compressive stress. Discussion This experimental in vitro study shows that multi-parametric MRI on isolated discs in vitro is not sensitive to compression or to the partial confined relaxation that followed the compression.

The aim of this study was to investigate the benefits arising from the use of a multiecho sequence for susceptibility-weighted phase imaging using a quantitative comparison with a standard single-echo acquisition. Four healthy adult volunteers were imaged on a clinical 3-T system using a protocol comprising two different three-dimensional susceptibility-weighted gradient-echo sequences: a standard single-echo sequence and a multiecho sequence. Both sequences were repeated twice in order to evaluate the local noise contribution by a subtraction of the two acquisitions. For the multiecho sequence, the phase information from each echo was independently unwrapped, and the background field contribution was removed using either homodyne filtering or the projection onto dipole fields method. The phase information from all echoes was then combined using a weighted linear regression. R2 ⁎ maps were also calculated from the multiecho acquisitions. The noise standard deviation in the reconstructed phase images was evaluated for six manually segmented regions of interest (frontal white matter, posterior white matter, globus pallidus, putamen, caudate nucleus and lateral ventricle). The use of the multiecho sequence for susceptibility-weighted phase imaging led to a reduction of the noise standard deviation for all subjects and all regions of interest investigated in comparison to the reference single-echo acquisition. On average, the noise reduction ranged from 18.4% for the globus pallidus to 47.9% for the lateral ventricle. In addition, the amount of noise reduction was found to be strongly inversely correlated to the estimated R2 ⁎ value ( R =−0.92). In conclusion, the use of a multiecho sequence is an effective way to decrease the noise contribution in susceptibility-weighted phase images, while preserving both contrast and acquisition time. The proposed approach additionally permits the calculation of R2 ⁎ maps.

Dose indicators such as the computed tomography dose index (CTDI) and dose-length product (DLP) were gathered for all routine abdomen-pelvis, chest and head examinations performed on all computed tomography (CT) scanners at a University Health Center (UHC) in Canada. These indicators were analysed and compared with the range of diagnostic reference levels (DRLs) suggested by Health Canada and with DRLs in other countries. Mean DLP values varied from one scanner to another, but mean values at the UHC (750 mGy cm(-1) for abdomen-pelvis CT, 349 mGy cm(-1) for chest CT and 1181 mGy cm(-1) for head CT) were all below the upper limit of the range of DRLs suggested by Health Canada. Local DRLs at the UHC were set to 810 mGy cm(-1) for abdomen-pelvis CT, 345 mGy cm(-1) for chest CT and 1205 mGy cm(-1) for head CT. Results, however, show the need for protocols revisions, since some scanners exhibit mean DLP values slightly below or above the upper limit of the range of DRLs suggested by Health Canada.

Results: With the simulated phantom, new artifacts that appear in the ROI images are caused by significant errors in the pilot reconstruction. These errors include excessive coarseness of the pilot image grid and beam-hardening artifacts. With the Catphan600 phantom, differences in the imaging model of the scanner and that of the iterative reconstruction algorithm cause dark border artifacts in the ROI images. Conclusions: Inexpensive pilot reconstruction techniques analytical algorithms, very-coarse-grid penalized likelihood are practical choices in many common cases. However, they may yield background images altered by edge degradation or beam hardening, inducing projection inconsistency in the data used for ROI reconstruction. The ROI images thus have significant streak and speckle artifacts, which adversely affect the resolution-to-noise compromise. In these cases, edgepreserving penalized-likelihood methods on not-too-coarse image grids prove to be more robust and provide the best ROI image quality. © 2010 American Association of Physicists in Medicine. DOI: 10.1118/1.3447722

Study Design. Clinical trial comparing image quality and entrance dose between Biospace EOS system, a new slot-scanning radiographic device, and a Fuji FCR 7501S computed radiography (CR) system for 50 patients followed for spinal deformities. Objective. Based on their physical properties, slot-scanners show the potential to produce image quality comparable to CR systems using less radiation. This article validates this assertion by comparing a new slot-scanner to a CR system through a wide-ranging evaluation of dose and image quality for scoliosis examinations. Summary of Background Data. For each patient included in this study, lateral and posteroanterior images were acquired with both systems. For each system, entrance dose was measured for different anatomic locations. Methods. Dose and image quality being directly related, comparable images were obtained using the same radiograph tube voltage on both systems while tube currents were selected to match signal-to-noise ratios on a phantom. Different techniques were defined with respect to patient's thickness about the iliac crests. Given dose amplitudes expected for scoliosis examinations, optically stimulated luminescence dosimeters were chosen as optimal sensors. Two radiologists and 2 orthopedists evaluated the images in a randomized order using a questionnaire targeting anatomic landmarks. Visibility of the structures was rated on a 4 level scale. Image quality assessment was analyzed using a Wilcoxon signed-rank tests. Results. Average skin dose was reduced from 6 to 9 times in the thoracoabdominal region when using the slot-scanner instead of CR. Moreover, image quality was significantly better with EOS for all structures in the frontal view (P < 0.006) and lateral view (P < 0.04), except for lumbar spinous processes, better seen on the CR (P < 0.003). Conclusion. We established that the EOS system offers overall enhanced image quality while reducing drastically the entrance dose for the patient.

Radiochromic film is a dosimeter of choice in applications requiring high spatial resolution, two dimensional measurements, or minimum perturbation of the beam fluence. Since the measurement uncertainty in Gafchromic film dosimetry is thought to be significant compared to that of ionization chambers, a rigorous method to evaluate measurement uncertainties is desired. This article provides a method that takes into account the correlation between fit parameters as well as single dose values in order to obtain accurate uncertainties in absolute and relative measurements. A complete portrait of all sources of uncertainty in Gafchromic film dosimetry is given. The parametrization of variance as a function of the number of averaged pixels is obtained in order to accurately predict the uncertainty as a function of the size of the region of interest. The choice of functional form for the sensitometric curve is based on four criteria and a convergence of global net optical density uncertainty to 0.0013 is demonstrated. A minimum number of 12 points is recommended to characterize the sensitometric curve to a sufficient precision on the uncertainty estimation. Uncertainty levels of 0.9% on absolute dose measurements and 0.45% on relative measurements are achieved using a 12-point calibration curve with 220 cGy and repeating measurements five times. Uncertainties of 0.8% and 0.4% are achievable when using 35 points during film characterization. Ignoring covariance terms is shown to lead to errors in the estimation of uncertainty.

Functional magnetic resonance imaging (fMRI) of the spinal cord has been the subject of intense research for the last ten years. An important motivation for this technique is its ability to detect non-invasively neuronal activity in the spinal cord related to sensorimotor functions in various conditions, such as after spinal cord lesions. Although promising results of spinal cord fMRI have arisen from previous studies, the poor reproducibility of BOLD activations and their characteristics remain a major drawback. In the present study we investigated the reproducibility of BOLD fMRI in the spinal cord of cats (N=9) by repeating the same stimulation protocol over a long period (approximately 2 h). Cats were anaesthetized with ketamine, and spinal cord activity was induced by electrical stimulation of cutaneous nerves of the hind limbs. As a result, task-related signals were detected in most cats with relatively good spatial specificity. However, BOLD response significantly varied within and between cats. This variability was notably attributed to the moderate intensity of the stimulus producing a low amplitude haemodynamic response, variation in end-tidal CO(2) during the session, low signal-to-noise ratio (SNR) in spinal fMRI time series and animal-specific vascular anatomy. Original contributions of the present study are: (i) first spinal fMRI experiment in ketamine-anaesthetized animals, (ii) extensive study of intra- and inter-subject variability of activation, (iii) characterisation of static and temporal SNR in the spinal cord and (iv) investigation on the impact of CO(2) end-tidal level on the amplitude of BOLD response.

Despite a relentless search for adequate and effective treatment, low back pain is one of the most prevalent (affecting up to 70% of people once during their lifetime) (1) and costly illness in today’s society (up to 50 billion dollars per year in the U.S.—not including earning and productivity losses) (2). It has long been recognized that the integrity of the intervertebral disc (IVD), part of a three-joint complex that determines the motion segment of the spine, is an essential component of normal spinal function. Alterations in the disc’s structure may cause back pain and referred pain with or without neurological impairment (3), in addition to significantly compromising the biomechanical integrity of the motion segment (4). While disc degeneration has been implicated as a major etiologic component of low back pain (5,6), there has been relatively little study in developing an objective, accurate, noninvasive diagnostic tool in the detection and quantification of matrix changes in early disc degeneration. Hence, the development of quantitative MR technology could allow early diagnostic and subsequent treatment of disc degeneration and thus reduce incidences of low back pain. IVDs are not uniform in composition, but consist of two distinct regions: The annulus fibrosus (AF) is a fibrocartilage and contains concentric lamellae rich in collagen, whereas the nucleus pulposus (NP) is a less structured gelatinous substance rich in proteoglycans (7). The disc is thought to resist compressive forces by a swelling pressure that exerts tensile forces on the fibrous collagen network (8). When a weakness in the collagenous network of the AF occurs, the swelling pressure within the NP may result in disc protrusion (9). With age, the swelling pressure of the NP decreases because of progressive degenerative changes that may begin as early as the third decade of life (10). These changes appear to be related to a loss of disc proteoglycans and/or to collagen network damage (10,11). The biomechanical function of the spine is reflected by its structural architecture, and therefore depends critically on the organization and composition of disc matrix components. Events that decrease glycosaminoglycan (GAG) content in the NP will impair its functional ability. Such a decrease occurs relatively early during adult human life and may be a prelude to tissue degeneration. Surgical treatments of degenerative disc disorders that are currently considered the “gold standard” include discectomy and spinal fusion. Although these surgical procedures produce a relatively good short-term clinical result in relieving pain, they alter the biomechanics of the spine, leading to further degeneration of the surrounding tissue and the discs at adjacent levels (12–14). The future of disc pathology treatment lies in the ability to treat the underlying cause at the level of the disc matrix rather than attempting to empirically treat the symptoms. However, little work has been done to systematically and objectively study quantitative MR technology as a method to investigate the pathologic and treatment-induced processes that occur in the IVD. Previous studies have evaluated the potential of quantitative MRI as a diagnostic tool of disc degeneration by attempting to correlate the MRI signal to disc tissue degeneration (15–20). T1 and T2 have been shown to decrease significantly in the NP with Thompson grade 4 degeneration, while the magnetization transfer (MT) increased (17). The apparent diffusion coefficient (ADC) has also been shown to decrease in the NP in a direction-dependent manner with disc degeneration (18,19). Correlation studies between MR parameters T1, T2, MT, and ADC have demonstrated that quantitative MRI reflects not only the NP matrix composition of the disc, but also the structural integrity of the NP matrix (17,18). Recently, the spin-lattice relaxation in the rotating frame, T1ρ, which detects low-frequency physicochemical interactions between water and extracellular matrix molecules, has been shown to decrease with disc degeneration and to correlate with the NP proteoglycan content (20). Studies using targeted enzyme digestion of the NP have allowed the determination of key correlations between MR parameters and the biochemical and mechanical properties of the NP tissue (21,22). Previous studies have shown that targeted collagen degradation of the NP in closed, static, and unloaded disc segments decreased T1, T2, and MT ration (MTR), and affected the ADC in a direction-dependent manner when compared with buffer-injected NPs (22). However, targeting the proteoglycan and/or hyaluronan integrity of the NP by trypsin and hyaluronidase treatment, respectively, while maintaining the matrix content constant did not significantly affect the MR parameters (22). Hence, enzymatic degradation of the NP tissue in a closed and static system influenced MR parameters in an enzyme-specific manner. Another study showed that T1, T2, and ADC correlated with the hydraulic permeability of the NP, measured by confined compression tests, while only diffusion correlated with the compressive modulus of the NP tissue (21). Thus, it was of interest to treat the NP tissue with trypsin under physiological compressive loading and determine its effect on the quantitative MRI, biochemical, and mechanical properties under physiological conditions. A recent study of compression loading in the physiological range for a period of only 2 hours demonstrated that MR parameters are sensitive to changes in the NP water content induced during loading (21). However, providing a more substantial mechanical challenge to the IVD tissue should better simulate physiological loading over a 16-hour day (23) and thus evaluate if compression enhances loss of proteins in the trypsin-digested disc tissue. Indeed, it was hypothesized that the compression loading conditions employed in this study would result in compositional and structural changes in both the NP and AF tissues that would be detectable with quantitative MRI, but that the enzymatic treatment would affect only the properties of the targeted NP tissue and not of its adjacent AF tissue. It was thus the purpose of the present work to establish the correlations between MR parameters and the biochemical and mechanical properties of the NP undergoing targeted trypsin digestion and axial compression at forces corresponding to 1 MPa loaded at 1 Hz for 16 hours in saline solution. The second aim of this work was to correlate changes in AF tissue due to mechanical loading to MR parameters and to determine if targeted enzymatic digestion of the NP affected the biochemical, mechanical, and MR properties of the adjacent AF tissue. Since T1ρ has recently been correlated to disc degeneration (20), the third goal was to evaluate the sensitivity of T1ρ not only to IVD composition but also to its structural integrity by evaluating its relationship with mechanical loading and biochemical composition.

The possibility of automatically navigating untethered microdevices or future nanorobots to conduct target endovascular interventions has been demonstrated by our group with the computer-controlled displacement of a magnetic sphere along a pre-planned path inside the carotid artery of a living swine. However, although the feasibility of propelling, tracking and performing real-time closed-loop control of an untethered ferromagnetic object inside a living animal model with a relatively close similarity to human anatomical conditions has been validated using a standard clinical Magnetic Resonance Imaging (MRI) system, little information has been published so far concerning the medical and technical protocol used. In fact, such a protocol developed within technological and physiological constraints was a key element in the success of the experiment. More precisely, special software modules were developed within the MRI software environment to offer an effective tool for experimenters interested in conducting such novel interventions. These additional software modules were also designed to assist an interventional radiologist in all critical real-time aspects that are executed at a speed beyond human capability, and include tracking, propulsion, event timing and closed-loop position control. These real-time tasks were necessary to avoid a loss of navigation control that could result in serious injury to the patient. Here, additional simulation and experimental results for microdevices designed to be targeted more towards the microvasculature have also been considered in the identification, validation and description of a specific sequence of events defining a new computer-assisted interventional protocol that provides the framework for future target interventions conducted in humans.

Object Conventional imaging demonstrates intertwined fibers of the cerebral white matter as a homogeneous substrate. Recently, diffusion tensor imaging has allowed 3D reconstruction of these fiber bundles. The goal of this study was to analyze the modifications of the association fibers induced by an arteriovenous malformation (AVM) in the parietotemporooccipital (PTO) associative area and their clinical significance. Methods The authors analyzed the long association fibers in seven patients harboring an AVM in or near the PTO region in relation with the fibers' clinical manifestation. The fibers include the arcuate fasciculus (AF), the occipitofrontal fasciculus (OFF), and the inferior longitudinal fasciculus (ILF). These structures were compared with the contra-lateral bundles. Results The modification of the tracts could establish a pattern signature depending on the specific location of the vascular malformation. There was a positive correlation between the degree of modifications of OFF and ILF fiber tracts and visual deficits. Alteration of the AF correlated with a speech disorder and the risk of postoperative deficits. Conclusions Diffusion tensor imaging enables in vivo dissection of fiber tracts coursing through the PTO area. Depending on the location of the AVMs, long association fibers are variously modified. These findings correlate with clinical manifestations and may predict outcome after surgery.

Rationale and Objectives To determine optimum spatial resolution when imaging peripheral arteries with magnetic resonance angiography (MRA). Materials and Methods Eight vessel diameters ranging from 1.0 to 8.0 mm were simulated in a vascular phantom. A total of 40 three-dimensional flash MRA sequences were acquired with incremental variations of fields of view, matrix size, and slice thickness. The accurately known eight diameters were combined pairwise to generate 22 "exact" degrees of stenosis ranging from 42% to 87%. Then, the diameters were measured in the MRA images by three independent observers and with quantitative angiography (QA) software and used to compute the degrees of stenosis corresponding to the 22 "exact" ones. The accuracy and reproducibility of vessel diameter measurements and stenosis calculations were assessed for vessel size ranging from 6 to 8 mm (iliac artery), 4 to 5 mm (femoro-popliteal arteries), and 1 to 3 mm (infrapopliteal arteries). Maximum pixel dimension and slice thickness to obtain a mean error in stenosis evaluation of less than 10% were determined by linear regression analysis. Results Mean errors on stenosis quantification were 8.8% ± 6.3% for 6- to 8-mm vessels, 15.5% ± 8.2% for 4- to 5-mm vessels, and 18.9% ± 7.5% for 1- to 3-mm vessels. Mean errors on stenosis calculation were 12.3% ± 8.2% for observers and 11.4% ± 15.1% for QA software ( P = .0342). To evaluate stenosis with a mean error of less than 10%, maximum pixel surface, the pixel size in the phase direction, and the slice thickness should be less than 1.56 mm 2 , 1.34 mm, 1.70 mm, respectively (voxel size 2.65 mm 3 ) for 6- to 8-mm vessels; 1.31 mm 2 , 1.10 mm, 1.34 mm (voxel size 1.76 mm 3 ), for 4- to 5-mm vessels; and 1.17 mm 2 , 0.90 mm, 0.9 mm (voxel size 1.05 mm 3 ) for 1- to 3-mm vessels. Conclusion Higher spatial resolution than currently used should be selected for imaging peripheral vessels.

STUDY DESIGN: The structural integrity of the nucleus pulposus (NP) of intervertebral discs was targeted by enzyme-specific degradations to correlate their effects to the magnetic resonance (MR) signal. OBJECTIVE: To develop quantitative MR imaging as an accurate and noninvasive diagnostic tool to better understand and treat disc degeneration. SUMMARY OF BACKGROUND DATA: Quantitative MR analysis has been previously shown to reflect not only the disc matrix composition, but also the structural integrity of the disc matrix. Further work is required to identify the contribution of the structural integrity versus the matrix composition to the MR signal. METHODS: The bovine coccygeal NPs were injected with either enzyme or buffer, incubated at 37 degrees C as static, unloaded and closed 3-disc segments, and analyzed by a 1.5-Tesla MR scanner to measure MR parameters. RESULTS: Collagenase degradation of the NP significantly decreased the relaxation times, slightly decreased the magnetization transfer ratio, and slightly increased the apparent diffusion coefficient. Targeting the proteoglycan and/or hyaluronan integrity by trypsin and hyaluronidase did not significantly affect the MR parameters, except for an increase in the apparent diffusion coefficient of the disc after trypsin treatment. CONCLUSIONS: Our results demonstrate that changes in the structural integrity of matrix proteins can be assessed by quantitative MR.

A clinical strength MRI and intact bovine caudal intervertebral discs were used to test the hypotheses that (1) mechanical loading and trypsin treatment induce changes in NMR parameters, mechanical properties and biochemical contents; and (2) mechanical properties are quantitatively related to NMR parameters. MRI acquisitions, confined compression stress-relaxation experiments, and biochemical assays were applied to determine the NMR parameters (relaxation times T1 and T2, magnetization transfer ratio (MTR) and diffusion trace (TrD)), mechanical properties (compressive modulus H(A0) and hydraulic permeability k(0)), and biochemical contents (H(2)O, proteoglycan and total collagen) of nucleus pulposus tissue from bovine caudal discs subjected to one of two injections and one of two mechanical loading conditions. Significant correlations were found between k(0) and T1 (r=0.75,p=0.03), T2 (r=0.78, p=0.02), and TrD (r=0.85, p=0.007). A trend was found between H(A0) and TrD (r=0.56, p=0.12). However, loading decreased these correlations (r=0.4, p=0.2). The significant effect of trypsin treatment on mechanical properties, but not on NMR parameters, may suggest that mechanical properties are more sensitive to the structural changes induced by trypsin treatment. The significant effect of loading on T1 and T2, but not on H(A0) or k(0), may suggest that NMR parameters are more sensitive to the changes in water content enhanced by loading. We conclude that MRI offers promise as a sensitive and non-invasive technique for describing alterations in material properties of intervertebral disc nucleus, and our results demonstrate that the hydraulic permeability correlated more strongly to the quantitative NMR parameters than did the compressive modulus; however, more studies are necessary to more precisely characterize these relationships.

The aim of this functional magnetic resonance imaging (fMRI) study was to compare regional brain activity in schizophrenia subjects with (FA+) and without (FA-) flat affect during the viewing of emotionally negative pictures. Thirteen FA+ subjects and 11 FA- subjects were scanned while being presented with a series of emotionally negative and neutral pictures. Experientially, the viewing of the negative pictures induced a negative emotional state whose intensity was significantly greater in the FA- group than in the FA+ group. Neurally, the Negative minus Neutral contrast revealed, in the FA- group, significant loci of activation in the midbrain, pons, anterior cingulate cortex, insula, ventrolateral orbitofrontal cortex, anterior temporal pole, amygdala, medial prefrontal cortex, and extrastriate visual cortex. In the FA+ group, this contrast produced significant loci of activation in the midbrain, pons, anterior temporal pole, and extrastriate visual cortex. When the brain activity measured in the FA+ group was subtracted from that measured in the FA- group, only the lingual gyrus was significantly activated. Perhaps in FA+ subjects an amygdaloid malfunction rendered the amygdala unable to correctly evaluate the emotional meaning of the pictures presented, thus preventing effective connectivity linking the amygdala to the brain regions implicated in the physiological and experiential dimensions of emotion. Alternatively, a disturbance of effective connectivity in the neural networks linking the midbrain and the medial prefrontal system may have been responsible for the quasi absence of emotional reaction in FA+ subjects, and the abnormal functioning of the medial prefrontal cortex and anterior cingulate cortex in the FA+ group.

A new scanning slit gas detector dedicated to orthopedic x-ray imaging is presented and evaluated in terms of its fundamental imaging characteristics. The system is based on the micromesh gaseous structure detector and achieves primary signal amplification through electronic avalanche in the gas. This feature, together with high quantum detection efficiency and fan-beam geometry, allows for imaging at low radiation levels. The system is composed of 1764 channels spanning a width of 44.8 cm and is capable of imaging an entire patient at speeds of up to 15 cm/s. The resolution was found to be anisotropic and significantly affected by the beam quality in the horizontal direction, but otherwise sufficient for orthopedic studies. As a consequence of line-by-line acquisition, the images contain some ripple components due to mechanical vibrations combined with variations in the x-ray tube output power. The reported detective quantum efficiency ! DQE" values are relatively low ! 0.14 to 0.20 at 0.5 mm −1 " as a consequence of a suboptimal collimation geometry. The DQE values were found to be unaffected by the exposure down to 7 ! Gy, suggesting that the system is quantum limited even for low radiation levels. A system composed of two orthogonal detectors is already in use and can produce dual-view full body scans at low doses. This device could contribute to reduce the risk of radiation induced cancer in sensitive clientele undergoing intensive x-ray procedures, like young scoliotic women. © 2005 American Association of Physicists in Medicine. # DOI: 10.1118/1.1876592$

A new scanning slit digital radiography system based on a gas microstrip detector is presented. The EOS device, manufactured by Biospace Instruments, is equipped with two orthogonal xenon detectors and can produce dual-view full body scans at speeds up to 15 cm / s . The detectors are operated at 6 atm and contain 1764 channels at a pitch of 254 μ m , with an avalanche signal amplification stage. The system can produce images with minimal dose requirements because of excellent scatter rejection and high quantum detection efficiency. Modulation transfer function (MTF) results for three beam qualities as well as comparisons with Monte Carlo simulations are reported. The resolution was found to be dependent on beam quality and on direction. In the horizontal direction, the measured MTF drops to half its full value at 1.0, 0.90 and 0.76 mm - 1 for beams having half value layers of 1.8, 3.4 and 5.1 mm of Al. In the vertical direction, the corresponding values are 0.97, 0.95 and 0.92 mm - 1 .

PURPOSE To evaluate imaging characteristics and artifacts of a nitinol stent with distal tantalum markers with computed tomography (CT) angiography and magnetic resonance (MR) angiography. MATERIALS AND METHODS A vascular phantom was built to simulate in-stent restenosis. A nitinol stent with tantalum markers (Luminexx stent) was evaluated with CT angiography in different orientations relative to the z-axis and with MR angiography in different positions relative to both B0 and the readout gradient. Stenosis measurements were compared with conventional digital subtraction angiography for both modalities. In-stent signal intensity obtained with different flip angles was assessed in two nitinol stents with distal markers (Luminexx stent and SMART stent) and one without markers (Memotherm-FLEXX stent). RESULTS Stenosis detection was not possible with CT angiography when the stent was perpendicular to the z-axis because of streak-like artifacts induced by tantalum markers. Stenosis evaluation with multiplanar reformation was accurate when the stent was in parallel and oblique orientations relative to the table axis. With MR angiography, metallic artifacts were mostly related to the stent orientation with B0, whereas orientation of the readout gradient had little influence. The mean error (overestimation) for stenosis measurements varied between 0.1% and 7.4% for CT imaging in parallel and oblique positions and 3.6% and 9.5% for MR imaging. Higher flip angles did not improve signal intensity inside the three stents tested. CONCLUSIONS CT and MR angiography can be used for evaluating the patency of stents with distal markers that are parallel or oblique relative to the table axis (iliac, carotid, or femoral stents). MR angiography is preferred if the stent is perpendicular to the table axis (renal stent).

The purpose of the present study was to compare the brain regions and systems that subserve lexical and sublexical processes in reading. In order to do so, three types of tasks were used: (i). silent reading of very high frequency regular words (lexical task); (ii). silent reading of nonwords (sublexical task); and, (iii). silent reading of very low frequency regular words (sublexical task). All three conditions were contrasted with a visual/phonological baseline condition. The lexical condition engaged primarily an area at the border of the left angular and supramarginal gyri. Activation found in this region suggests that this area may be involved in mapping orthographic-to-phonological whole word representations. Both sublexical conditions elicited significantly greater activation in the left inferior prefrontal gyrus. This region is thought to be associated with sublexical processes in reading such as grapheme-to-phoneme conversion, phoneme assembly and underlying verbal working memory processes. Activation in the left IFG was also associated with left superior and middle temporal activation. These areas are thought to be functionally correlated with the left IFG and to contribute to a phonologically based form of reading. The results as a whole demonstrate that lexical and sublexical processes in reading activate different regions within a complex network of brain structures.

Objective To evaluate the change in osteoarthritic (OA) knee cartilage volume over a two-year period with the use of magnetic resonance imaging (MRI) and to correlate the MRI changes with radiologic changes. Methods Thirty-two patients with symptomatic knee OA underwent MRI of the knee at baseline and at 6, 12, 18, and 24 months. Loss of cartilage volumes were computed and contrasted with changes in clinical variables for OA and with standardized semiflexed knee radiographs at baseline at 1 and 2 years. Results Progression of cartilage loss at all followup points was statistically significant (P 15% of global cartilage loss; n = 11) over the 2 years of study. At baseline, there was a greater proportion of women (P = 0.001), a lower range of motion (P = 0.01), a greater circumference and higher level of pain (P = 0.05) and stiffness in the study knee, and a higher body mass index in the fast progressor group compared with the slow progressor group. No statistical correlation between loss of cartilage volume and radiographic changes was seen. Conclusion Quantitative MRI can measure the progression of knee OA precisely and can help to identify patients with rapidly progressing disease. These findings indicate that MRI could be helpful in assessing the effects of treatment with structure-modifying agents in OA.

In rapid streams of visual stimuli, identification of a first target interferes with identification of a second target presented within the next half second (the attentional blink or AB). It has been suggested that rapid perceptual decisions under masking interference involve interactions between frontal and posterior cortex. We investigated the neural correlates of the AB using functional magnetic resonance imaging (fMRI). Twelve subjects viewed rapid streams of black letters in which were embedded two white target letters (T1 and T2) separated by either 300 or 700 ms. As expected, fewer correct T2 identifications were observed in the short-delay condition. Corresponding fMRI statistical images showed increased activation in inferotemporal and posterior parietal cortex, but also in lateral frontal cortex and cerebellum in the short-delay condition suggesting that these brain regions are associated with perceptual decisions under masking interference.

Emotional development is indisputably one of the cornerstones of personality development during infancy. According to the differential emotions theory (DET), primary emotions are constituted of three distinct components: the neural–evaluative, the expressive, and the experiential. The DET further assumes that these three components are biologically based and functional nearly from birth. Such a view entails that the neural substrate of primary emotions must be similar in children and adults. Guided by this assumption of the DET, the present functional magnetic resonance imaging study was conducted to identify the neural correlates of sad feelings in healthy children. Fourteen healthy girls (aged 8–10) were scanned while they watched sad film excerpts aimed at externally inducing a transient state of sadness (activation task). Emotionally neutral film excerpts were also presented to the subjects (reference task). The subtraction of the brain activity measured during the viewing of the emotionally neutral film excerpts from that noted during the viewing of the sad film excerpts revealed that sad feelings were associated with significant bilateral activations of the midbrain, the medial prefrontal cortex (Brodmann area [BA] 10), and the anterior temporal pole (BA 21). A significant locus of activation was also noted in the right ventrolateral prefrontal cortex (BA 47). These results are compatible with those of previous functional neuroimaging studies of sadness in adults. They suggest that the neural substrate underlying the subjective experience of sadness is comparable in children and adults. Such a similitude provides empirical support to the DET assumption that the neural substrate of primary emotions is biologically based.

Background The ability to voluntarily self-regulate negative emotion is essential to a healthy psyche. Indeed, a chronic incapacity to suppress negative emotion might be a key factor in the genesis of depression and anxiety. Regarding the neural underpinnings of emotional self-regulation, a recent functional neuroimaging study carried out by our group has revealed that the dorsolateral prefrontal cortex (DLPFC) and anterior cingulate cortex are involved in voluntary suppression of sexual arousal. As few things are known, still, with respect to the neural substrate underlying volitional self-regulation of basic emotions, here we used functional magnetic resonance imaging to identify the neural circuitry associated with the voluntary suppression of sadness. Methods Twenty healthy female subjects were scanned during a Sad condition and a Suppression condition. In the Sad condition, subjects were instructed to react normally to sad film excerpts whereas, in the Suppression condition, they were asked to voluntarily suppress any emotional reaction in response to comparable stimuli. Results Transient sadness was associated with significant loci of activation in the anterior temporal pole and the midbrain, bilaterally, as well as in the left amygdala, left insula, and right ventrolateral prefrontal cortex (VLPFC) (Brodmann area [BA] 47). Correlational analyses carried out between self-report ratings of sadness and regional blood oxygen level dependent (BOLD) signal changes revealed the existence of positive correlations in the right VLPFC (BA 47), bilaterally, as well as in the left insula and the affective division of the left anterior cingulate gyrus (BA 24/32). In the Suppression condition, significant loci of activation were noted in the right DLPFC (BA 9) and the right orbitofrontal cortex (OFC) (BA 11), and positive correlations were found between the self-report ratings of sadness and BOLD signal changes in the right OFC (BA 11) and right DLPFC (BA 9). Conclusions These results confirm the key role played by the DLPFC in emotional self-regulation. They also indicate that the right DLPFC and right OFC are components of a neural circuit implicated in voluntary suppression of sadness.

To assess the ability to control the steering of a modified guidewire actuated by the magnetic force of a magnetic resonance imaging system with additional gradient coils for selective arterial catheterization in rabbits.Selective catheterizations of the right renal artery, left renal artery, superior mesenteric artery, and iliac artery were performed on two rabbits. A 3D magnetic force was applied onto a magnetic bead placed at the tip of a guidewire. The ability of the guidewire to advance in the aorta without entering the side branches when the magnetic force was not applied was also evaluated. Steering of the guidewire was combined with a dedicated tracking system and its position was registered on the 3D model of a magnetic resonance angiography (MRA).The magnetic catheterization of the renal arteries was successful and showed reproducibility. Superior mesenteric artery and iliac artery showed that the catheterization was feasible. These two arteries were difficult to visualize on MRA, making catheterization and setting the direction of the force more difficult. There was no inadvertent catheterization of side vessels when the guidewire was advanced with magnetic steering despite the hook shape at the tip of the guidewire caused by the alignment of the bead anisotropy with the permanent magnetic field.This first evaluation of selective catheterization of aortic branches with a magnetic guidewire provided a successful steering in the less angled side branches and this modified guidewire was advanced in the aorta without inadvertent selective catheterization when manipulated without magnetic actuation.

Various lines of evidence indicate that men generally experience greater sexual arousal (SA) to erotic stimuli than women. Yet, little is known regarding the neurobiological processes underlying such a gender difference. To investigate this issue, functional magnetic resonance imaging was used to compare the neural correlates of SA in 20 male and 20 female subjects. Brain activity was measured while male and female subjects were viewing erotic film excerpts. Results showed that the level of perceived SA was significantly higher in male than in female subjects. When compared to viewing emotionally neutral film excerpts, viewing erotic film excerpts was associated, for both genders, with bilateral blood oxygen level dependant (BOLD) signal increases in the anterior cingulate, medial prefrontal, orbitofrontal, insular, and occipitotemporal cortices, as well as in the amygdala and the ventral striatum. Only for the group of male subjects was there evidence of a significant activation of the thalamus and hypothalamus, a sexually dimorphic area of the brain known to play a pivotal role in physiological arousal and sexual behavior. When directly compared between genders, hypothalamic activation was found to be significantly greater in male subjects. Furthermore, for male subjects only, the magnitude of hypothalamic activation was positively correlated with reported levels of SA. These findings reveal the existence of similarities and dissimilarities in the way the brain of both genders responds to erotic stimuli. They further suggest that the greater SA generally experienced by men, when viewing erotica, may be related to the functional gender difference found here with respect to the hypothalamus. Hum. Brain Mapping 16:1–13, 2002. © 2002 Wiley‐Liss, Inc.

Two studies examined the contribution of human frontal cortical areas to the programming of trains of repetitive movements. The first study compared the performance of patients with unilateral frontal excisions, unilateral temporal excisions and controls on the speed of initiation of discrete vs. sequential tapping movements to visual stimuli. The frontal group showed normal initiation times in single taps and a normal execution (pace and accuracy) in sequential taps but they were slower than the other groups at initiating sequential taps indicating a sequence programming problem for repetitions of a single response. A second study examined the functional anatomy of single and sequential taps in eight control subjects using fMRI. Subjects performed flexion/extension movements of the right thumb at either 1 movement/s or as trains of four closely spaced movements at a rate of 1 train/4 s. Statistical analyses revealed that primary sensorimotor cortex and a dorsolateral premotor cortex region were activated in both conditions. Medial frontal activation was not significant in discrete movements but was clearly present in sequential movements and involved SMA and cingulate regions bilaterally. In addition, two other dorsolateral premotor foci of activation were observed in the sequential taps condition. Results from these two experiments converge toward establishing a significant role of dorsolateral and medial premotor regions in the programming of trains of repetitive responses.

An important issue regarding the neural basis of major depression is whether the functional brain changes associated with the affect disturbance seen in this syndrome are similar to those that accompany transient sadness in normal subjects. To address this question, we carried out an fMRI study using an emotional activation paradigm. Brain activity associated with passive viewing of an emotionally laden film clip aimed at inducing a transient state of sadness was contrasted with that associated with passive viewing of an emotionally neutral film clip in patients suffering from unipolar depression and in normal control subjects. Results showed that transient sadness produced significant activation in the medial and inferior prefrontal cortices, the middle temporal cortex, the cerebellum and the caudate in both depressed and normal subjects. They also revealed that passive viewing of the emotionally laden film clip produced a significantly greater activation in the left medial prefrontal cortex and in the right cingulate gyrus in depressed patients than in normal control subjects. These findings suggest that these two cortical regions might be part of a neural network implicated in the pathophysiology of major depression. Taken together, these results strongly support the view that activation paradigms represent an extremely useful and powerful way of delineating the functional anatomy of the various symptoms that characterize major depression.

Evidence is presented for the production of prompt J/psi mesons (not originating in b-hadron decays) in hadronic Z(0) decays. Using a sample of 3.6 million hadronic events, 24 prompt J/psi candidates are identified from their decays into e(+)e(-) and mu(+)mu(-)pairs. The background is estimated to be 10.2+/-2.0 events. The following branching ratio for prompt J/psi production is obtained: Br(Z(0)-->prompt J/psi+X)=(1.9+/-0.7+/-0.5+/-0.5). 10(-4), where the first error is statistical, the second systematic and the third error accounts for uncertainties in the prompt J/psi production mechanism.

The spin composition of Λ Λ , ΛΛ and Λ Λ pairs at low invariant mass values has been measured for the first time in multihadronic Z0 decays with the OPAL detector at LEP. No single spin state has been observed in the Λ Λ sample, verifying that the low mass enhancement in this sample, attributed to local baryon number compensation, is not a resonance state. The fraction of the spin 1 contribution to the Λ Λ pairs was found to be consistent with the value 0.75, as expected from a statistical spin mixture. This may be the net effect of many different QCD processes which contribute to the hyperon anti-hyperon pair production. The spin composition of the identical ΛΛ and Λ Λ pairs, well above threshold, is found to be similar to that of the Λ Λ sample. A ΛΛ emitter dimension is estimated from the data assuming the onset of the Pauli exclusion principle near threshold.

A search for charginos and neutralinos, predicted by supersymmetric theories, has been performed using a data sample of 2.6 pb−1 at a centre-of-mass energy of S=130 GeV and 2.6 pb−1 at 136 GeV collected with the OPAL detector at LEP during November 1995. No candidate events were observed. The 95% C.L. lower limit on the lightest chargino mass in the Minimal Supersymmetric Standard Model is 65.4 GeV if the universal scalar mass m0 is greater than 1 TeV, and 58.7 GeV for the smallest m0 compatible with slepton and sneutrino mass limits obtained at centre-of-mass energies near the Z peak. These limits were obtained under the conditions that the lightest chargino is heavier than the lightest neutralino by more than 10 GeV and tan β is larger than 1.5. The results of a model independent search for charginos and neutralinos are also given.

A search for scalar particles and neutralinos such as those predicted by supersymmetric models has been performed using a data sample of 4.4 million hadronic Z 0 decays recorded by the OPAL detector at the e + e − collider LEP. The production of such particles typically leads to event topologies consisting of an acoplanar pair of jets, or of a mono-jet, accompanied by sizeable missing energy owing to neutrinos and other undetectable neutral particles. Limits are obtained, at the 95% confidence level, on the masses and production rates of scalar particles produced in association with the Z 0 . Limits are also placed on neutralino production, for which an additional possible signature is also studied, events containing a single observed photon. Within the Minimal Supersymmetric Standard Model, the mass of the lightest neutralino is found to be larger than 12.5 GeV/ c 2 at 95% C.L., provided that tan β is larger than 1.5.