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15 results on '"Gombert, Karine"'

3. Neutralizing Aptamers from Whole-Cell SELEX Inhibit the RET Receptor Tyrosine Kinase

Author

Cerchia Laura, Ducongé Frédéric, Pestourie Carine, Boulay Jocelyne, Aissouni Youssef, Gombert Karine, Tavitian Bertrand, de Franciscis Vittorio, and Libri Domenico

Subjects
Biotechnology, Cancer Biology, Biology (General), QH301-705.5
Abstract

Targeting large transmembrane molecules, including receptor tyrosine kinases, is a major pharmacological challenge. Specific oligonucleotide ligands (aptamers) can be generated for a variety of targets through the iterative evolution of a random pool of sequences (SELEX). Nuclease-resistant aptamers that recognize the human receptor tyrosine kinase RET were obtained using RET-expressing cells as targets in a modified SELEX procedure. Remarkably, one of these aptamers blocked RET-dependent intracellular signaling pathways by interfering with receptor dimerization when the latter was induced by the physiological ligand or by an activating mutation. This strategy is generally applicable to transmembrane receptors and opens the way to targeting other members of this class of proteins that are of major biomedical importance.

Published
2005
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10. Membrane-type 4 matrix metalloproteinase (MT4-MMP) induces lung metastasis by alteration of primary breast tumour vascular architecture

Author

Chabottaux, Vincent, primary, Ricaud, Stéphanie, additional, Host, Laurent, additional, Blacher, Silvia, additional, Paye, Alexandra, additional, Thiry, Marc, additional, Garofalakis, Anikitos, additional, Pestourie, Carine, additional, Gombert, Karine, additional, Bruyere, Françoise, additional, Lewandowsky, Daniel, additional, Tavitian, Bertrand, additional, Foidart, Jean-Michel, additional, Duconge, Frédéric, additional, and Noel, Agnès, additional

Published
2009
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11. Fluorine-18-Labeled Phospholipid Quantum Dot Micelles for in Vivo Multimodal Imaging from Whole Body to Cellular Scales

Author

Ducongé, Frédéric, primary, Pons, Thomas, additional, Pestourie, Carine, additional, Hérin, Laurence, additional, Thézé, Benoît, additional, Gombert, Karine, additional, Mahler, Benoît, additional, Hinnen, Françoise, additional, Kühnast, Bertrand, additional, Dollé, Frédéric, additional, Dubertret, Benoît, additional, and Tavitian, Bertrand, additional

Published
2008
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12. Sélection d’aptamères pour l’imagerie moléculaire

Author

Pestourie, Carine, primary, Gombert, Karine, additional, Cerchia, Laura, additional, Herin, Laurence, additional, Theze, Benoît, additional, Boulay, Jocelyne, additional, Aissouni, Youssef, additional, de Franciscis, Vittorio, additional, Libri, Domenico, additional, Tavitian, Bertrand, additional, and Duconge, Frédéric, additional

Published
2007
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14. PET imaging of medullary thyroid carcinoma in MEN2A transgenic mice using 6-[18F]F- L-DOPA.

Author

Pestourie, Carine, Thézé, Benoît, Kuhnast, Bertrand, Helleix, Stéphane, Gombert, Karine, Dollé, Frédéric, Tavitian, Bertrand, and Ducongé, Frédéric

Subjects
MEDICAL imaging systems, POSITRON emission tomography, DIAGNOSTIC imaging, THYROID cancer, CANCER diagnosis, TRANSGENIC mice, ANIMAL experimentation
Abstract

6-[18F]Fluoro-3,4-dihydroxy- L-phenylalanine (6-[18F]F- L-DOPA) is increasingly used for PET imaging of neuroendocrine tumours. In this study, we investigated the use of 6-[18F]F- L-DOPA to detect and to monitor the progression of medullary thyroid carcinoma (MTC) in a genetically engineered mouse model of multiple endocrine neoplasia type 2A (MEN2A). Dynamic [18F]FDG and 6-[18F]F- L-DOPA small animal PET scans were acquired during 60 or 90 min in 8- to 20-month-old MEN2A transgenic mice. The kinetics of 6-[18F]F- L-DOPA, standardized uptake values (SUV) at 60 min and tumour volumes were recorded. The detection of MTCs using PET was confirmed by autopsy and histological analysis. 6-[18F]F- L-DOPA performs better than [18F]FDG for MTC detection in this transgenic mouse model. Uptake kinetics of 6-[18F]F- L-DOPA in MTCs are very different between mice but, in all cases, high contrast could be observed. Furthermore, 6-[18F]F- L-DOPA can detect tumours with sizes (1.8 mm3) that are near the resolution limit of PET, whereas they were undetectable by autopsy at the macroscopic level. 6-[18F]F- L-DOPA PET imaging can monitor the progression of MTCs in a genetically engineered mouse model. [ABSTRACT FROM AUTHOR]

Published
2010
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15. In Vitro and In Vivo Imaging of Fluorescent Aptamers.

Author

Théodorou I, Quang NN, Gombert K, Thézé B, Lelandais B, and Ducongé F

Subjects
Animals, Cell Line, Tumor, Disease Models, Animal, Heterografts, Humans, Imaging, Three-Dimensional methods, In Vitro Techniques, Mice, Mice, Nude, Microscopy, Video methods, Neoplasms diagnosis, Neoplasms metabolism, Staining and Labeling, Time-Lapse Imaging methods, Tomography, Optical methods, Aptamers, Nucleotide chemistry, Aptamers, Nucleotide metabolism, Fluorescent Dyes chemistry, Molecular Imaging methods
Abstract

Fluorescence imaging techniques could be used in different ways to study the interaction of aptamers with biological systems from cell culture to animal models. Here, we present the methods developed in our laboratory for fluorescently labeled aptamers, study their internalization inside living cells using time-lapse microscopy, and monitor their biodistribution in mice bearing subcutaneous xenograft tumors using planar fluorescence imaging and fluorescence diffuse optical tomography (fDOT).

Published
2016
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