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Using force tracing and nano-indentation techniques based on atomic force microscopy, the promoting effect of Tf on T7-modified nano-drug transport was investigated at a single-particle and single-cell level in real time.

The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals. Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis, the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive. Here, we utilized a novel force tracing technique based on atomic force microscopy(AFM) to record the process of single viral entry into host cell. We revealed that the force of the rabies virus internalization distributed at (65±25) pN, and the time was identified by two peaks with spacings of (237.2±59.1) and (790.3±134.4) ms with the corresponding speed of 0.12 and 0.04 µm/s, respectively. Our results provide insight into the effects of viral shape during the endocytosis process. This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.Supplementary material is available in the online version of this article at 10.1007/s40242-022-2069-y.

Medical image fusion technology can integrate complementary information from different modality medical images to provide a more complete and accurate description of the specific diagnosed object, which is very helpful for image-guided clinical diagnosis and treatment. This paper proposes an effective brain image fusion framework based on improved rolling guidance filter (IRGF). Firstly, input images are decomposed into base layers and detail layers using the IRGF and Wiener filter. Secondly, the visual saliency maps of the input image are computed by pixel-level saliency value, and the weight maps of detail layers are constructed by max-absolute strategy and are further smoothed with Gaussian filter, the purpose of which is to make the fused image appear more naturally and more suitable for human visual perception. Lastly, base layers are fused by visual saliency map based fusion rule and the corresponding weight maps from detail layers are fused by the weighted least squares optimization scheme. Experimental results testify that our method is superior to some state-of-the-art methods in both subjective and objective assessments.

Migratory birds spend several months in their breeding grounds in sympatry with local resident birds and relatively shorter periods of time at stopover sites. During migration, parasites may be transmitted between migratory and resident birds. However, to what extent they share these parasites remains unclear. In this study, we compared the assemblages of haemosporidian parasites in migratory, resident, and passing birds, as well as the correlations between parasite assemblages and host phylogeny. Compared with passing birds, migratory birds were more likely to share parasites with resident birds. Shared lineages showed significantly higher prevalence rates than other lineages, indicating that common parasites are more likely to spill over from the current host to other birds. For shared lineages, the prevalence was significantly higher in resident birds than in migratory birds, suggesting that migratory birds pick up parasites at their breeding ground. Among the shared lineages, almost two-thirds presented no phylogenetic signal in their prevalence, indicating that parasite transmission among host species is weakly or not correlated with host phylogeny. Moreover, similarities between parasite assemblages are not correlated with either migration status or the phylogeny of hosts. Our results show that the prevalence, rather than host phylogeny, plays a central role in parasite transmission between migratory and resident birds in breeding grounds.

Wind erosion is a global environmental problem and affects the sustainable use of land soil. The current efforts in wind erosion modeling mainly focus on local scales, yet very few studies have attempted to quantify the soil losses by wind on a large scale. Here, we proposed a distributed version of the revised wind erosion equation model (DRWEQ) to assess the spatial and temporal variations of wind erosion globally. The DRWEQ model used meteorological, soil, topographic, and remote sensing data to simulate global wind erosion from 2001 to 2010. The results showed that (a) the areas of wind erosion in Africa and Asia accounted for approximately 62% of the global wind erosion area but accounted for 91% of the global total soil loss; (b) global wind erosion showed a decreasing tendency during the research period – the wind erosion with a trend of intensification occupied 40.62% of the global wind erosion area while about 59.38% of the global wind erosion area showed a weakening trend; and (c) the monthly dynamics of the wind erosion were closely correlated with the combined effects of weather factors and vegetation coverage. The soil loss rates were lower in summer and reached the peak from January to April. The method presented in this study was developed based on the tradeoff of accuracy and availability of global data, and has the potential for predicting wind erosion from regional to global scales.

The popularity of RNA nanoparticles (RNPs) has risen rapidly during the past decade due to the development of RNA nanotechnology. Understanding the fast dynamic process of cell entry and intracellular delivery of RNPs is essential for the design of intelligent therapeutic RNA nano-drugs and mRNA vaccines.How the interaction between the membrane and target ligand of RNPs influences the cell entry, and how the dynamic mechanism of RNPs takes place in different organelles remain ill-defined. Herein, the cell entry of Antimir21-RNP-Apt is monitored using a force tracing technique with a high spatiotemporal resolution at the single particle level, the specific interaction of Apt and EGFR promotes the cell entry efficiency and achieves long-lasting curative effects. Furthermore, the intracellular delivery pathway through different organelles is discovered using fluorescence tracking, and the low motility in early endosomes and the high motility in late endosomes are analyzed. This report provides key strategies for engineering RNA nanomedicines and facilitating clinical translation.

The design and synthesis of low-cost and efficient bifunctional electrocatalysts for water splitting are critical and challenging. Hereby, a bimetallic phosphide embedded in a N and P co-doped porous carbon (FeCoP2@NPPC) material was synthesized by using sustainable biomass-derived N- and P-containing carbohydrates and non-noble metal salts as precursors. The obtained material exhibits good catalytic activities in hydrogen evolution reaction (HER), oxygen evolution reaction (OER), and overall water splitting. The bimetallic alloy phosphide (FeCoP2) is the active site for electrocatalysis. Theoretical calculation indicates that the sub-layer Fe atoms and top-layer Co atoms in FeCoP2 exhibit a synergistic effect for enhanced electrocatalytic performance. The carbon matrix around the FeCoP2 can prevent the corrosion during the catalytic reactions. The hierarchically porous structure of the FeCoP2@NPPC material can promote the transfer of electrons and electrolyte, and increase the contact area of the active sites and electrolytes. N- and P-containing functionalities improve the wetting and conductivity properties of the porous carbon. Due to the synergistic effects, FeCoP2@NPPC requires a low overpotential of 114 and 150 mV at the current density of 10 mA cm-2 for HER in 0.5 M H2SO4 and 1.0 M KOH, and an overpotential of 236 mV for OER in 1.0 M KOH solution, which are much lower than those of FeP@NPPC and CoP@NPPC. Based on the density functional theory calculation, FeCoP2 yields the smallest Gibbs free energy change of rate-determining step among the samples, which leads to better electrochemical performances. In addition, when FeCoP2@NPPC was used as a bifunctional catalyst in water splitting, the electrolyzer needed a low voltage of 1.60 V to deliver the current density of 10 mA cm-2. Furthermore, this work provides a strategy for preparing sustainable, stable, and highly active electrocatalysts for water splitting.

Ultrathin fibers can increase the contact area between adsorbents and seawater during the uranium extraction process; however, their construction usually aggravates the complex spinning technology and lowers their mechanical strength. Meanwhile, high strength and antifouling ability are essential for ocean adsorbents to withstand the complex natural environment and microbial systems. Herein, we design high-strength and anti-biofouling poly(amidoxime) nanofiber membranes (HA-PAO NFMs) via a supramolecular crosslinking. Bacterial cellulose supplies the NFMs with ultrathin fiber structure, and large amounts of adsorption ligands are immobilized on the framework via the crosslinking with antibacterial ions. Thus, different from other fibers, HA-PAO NFMs achieve ultrathin diameter (20-30 nm), high BET area (51 m

The HAIYPRH (T7) peptide has been widely used as a ligand for constructing tumor-targeted nanodrug delivery systems since it can target the transferrin receptor (TfR) and then enter cells easily with the help of transferrin (Tf). However, the dynamic mechanism by which transferrin promotes the entry of T7-conjugated nanostructures into cells remains unclear. Herein, a force tracing technique based on atomic force microscopy (AFM) was used to track the ultrafast dynamic process of a T7-conjugated gold nanoparticle (AuNP-T7) entering a cell at the single-particle level in real time. Tf helped decrease the endocytosis force and increase the endocytosis speed of AuNP-T7 in A549 cells. However, Tf only increased the endocytosis speed of AuNP-T7 in HeLa cells. In contrast, in Vero cells without TfR overexpression, Tf decreased the endocytosis speed. This report provides important insights for redesigning and developing T7-conjugated nanodrug carriers in targeted nanodrug delivery systems.

A targeted nano-drug delivery system has provided great potential and benefits to the diagnosis and therapy of cancers. Cell entry is a critical step for taking effect of the targeted nano-drug. In this report, the dynamics of delivering a single aptamer targeted polyamindoamine-camptothecin-AS1411 (PAMAM-CPT-AS1411) nano-drug into cells was investigated using a force tracing technique based on atomic force microscopy. The results show that the specific interaction of AS1411 and nucleolin, which is overexpressed on cancer cells, enhances the efficiency of the PAMAM-CPT-AS1411 cell entry. Moreover, the specific interaction induced receptor-mediated endocytosis prolongs the duration and decreases the speed of a single PAMAM-CPT-AS1411 cell entry, which is helpful to understand the targeted nano-drugs prolonging the therapeutic drug level. However, the required force for PAMAM-CPT-AS1411 cell entry is not changed. This report will provide a novel and potential method for achieving the precise dynamics of targeted nano-drug delivery.

How to effectively break down market segmentation and build a sustainable and unified domestic market has become critical to achieving high-quality development in the Chinese economy nowadays. This study examines the effects and mechanisms of improved judicial independence on the development of larger and more sustainable domestic supply chains, using a sample of Chinese enterprises from 2011 to 2016 and a quasi-natural experiment of local judicial reforms. We find that, after the establishment of local circuit courts, the distribution distance of a firm’s supply chain increases significantly. The mechanism analysis suggests that the increase in distribution distance in the domestic supply chain is due to the breakdown of market segmentation resulting from the reduction in local judicial protectionism and the improvement in the quality of local justice after the establishment of circuit courts. Further tests show that the impact of improved judicial independence on the domestic supply chain is most pronounced among small and manufacturing non-state-owned enterprises and those from less competitive industries. Overall, the findings of this paper provide important insights into developing large and sustainable supply chains via breaking down market segmentation, thereby promoting long-term economic growth.

The potential of converting traditional biomass into low-cost HER catalysts has broad application prospects. In this paper, fungus is used as a carbon-based carrier. The bimetallic catalyst RuM-C (M = V, Mo, W, Zn, Cu) was synthesized under inert gas protection at high temperature. The order of electrocatalytic activity is RuV-C > RuZn-C > RuW-C > RuMo-C > Ru-C > RuCu-C > BF-C, which indicates that RuV-C exhibits excellent HER activity. Due to its irregular sheet structure, the specific surface area of the catalyst is increased. Impressively, it exhibits extremely high catalytic activity for HER in 1 M KOH due to favorable kinetics and excellent specific activity. Consequently, the prepared RuV-C exhibited excellent and stable HER activity compared Ru-C with a low overpotential of 65.78 mV at the current densities of 10 mA cm−2 and Tafel slope of 45.26 mV dec−1. The potential only decreased by 88 mV after 24 h of continuous testing, which indicates that the catalyst has outstanding stability. This work will provide positive inspiration for the promotion of a new Ru-based biomass HER electrocatalyst.

Infections by the Ebola virus (EBOV) rapidly cause fatal hemorrhagic fever in humans. Viral entry into host cells is the most critical step in infection and an attractive target for therapeutic intervention. Herein, the invagination behavior and entry dynamics of filamentous Ebola virus-like particles (EBO-VLPs) were investigated using a force tracing technique based on atomic force microscopy and single-particle fluorescence tracking in real time. The filamentous EBOV-VLPs might enter cells in both horizontal and vertical modes, and the virus-receptor interactions during endocytic uptake were analyzed. In addition, molecular dynamics simulations and engulfment energy analysis further depicted EBO-VLP entry in the horizontal and vertical directions and suggested that internalization in the vertical direction requires a larger force and more time. This report provides useful information for further revealing the mechanism of viral infection, which is important for understanding viral pathogenesis.

Gold nanocages (Au NCs), as drug carriers, have been widely applied for cancer diagnosis and photothermal therapy (PTT). Transmembrane transporting efficacy of Au NCs is the fundamental and important issue for their use in PTT. Herein, we used a force tracing technique based on atomic force microscopy to track the dynamic transmembrane process of Au NCs at the single-particle level in real time. Meanwhile, we measured and compared the dynamic parameters of Au NCs with sizes of 50 and 100 nm usually used as nanodrug carriers of PTT. It is concluded that the 50 nm Au NC transmembrane transporting needs smaller force and shorter duration with a much faster speed. However, both the 50 and 100 nm Au NC transmembrane transporting depends on the caveolin-mediated endocytosis, clathrin-mediated endocytosis, and macropinocytosis, which was also confirmed by confocal fluorescence imaging. This report will provide a potential technique for screening nanodrug carriers from the perspective of transmembrane transporting efficacy.

Herein, strongly coupled Mo2C and Ni nanoparticles with in-situ formed interfaces encapsulated by porous carbon nanofibers (Ni-Mo2C-CNF) have been rationally fabricated via pyrolyzing electrospinning polyvinyl alcohol fibers containing hydrothermally obtained NiMoO4 under Ar atmosphere and applied as high-performance and stable electrocatalyst for HER in alkaline electrolytes. Powered by NiMoO4 as homologous bimetallic precursor, the Ni-Mo2C-CNF possesses numerous in-situ formed Ni-Mo2C interfaces, which facilitates the synergistic effect between Ni and Mo2C, improving the conductivity and thus boosting the electrocatalytic performance towards HER. In the meantime, the porous carbon nanofibers with well encapsulated Ni-Mo2C active components stacks, constituting conductive network, which promotes the mass transport, electron transfer, active sites exposure and electrocatalytic stability. As a result, the Ni-Mo2C-CNF features prominently in HER, as it demands a low overpotential of 196 mV but is able to stably yield the current density of 10 mA cm−2 with a small Tafel plot of 54.7 mV dec−1. The method demonstrated in our work to synthesize bimetallic heterostructured materials will offer valuable inspiration to construct promising non-precious electrocatalysts for diverse vital renewable energy applications.

Vertical helically baffled shell-and-tube heat exchangers (HBHXs) occupy smaller ground areas compared to horizontal HBHXs and have been used as propane condensers in liquefied natural gas (LNG) plants. The heat transfer characteristics of propane in the shell side of vertical HBHX were investigated in the present study. The flow patterns and heat transfer coefficients in the shell side of a vertical HBHX test sample were obtained, and the results were compared with the existing data of the horizontal HBHX. The experimental conditions cover mass flux of 20–40 kg m−2 s−1, heat flux of 3.0∼7.0 kW m−1, and vapor quality from 0.2 to superheated. It is found that the variation trend of propane heat transfer coefficient with the increase of vapor quality in the vertical HBHX shell side is similar to that of the horizontal one. However, opposite to the horizontal HBHX, the heat transfer coefficient increases with the rise of heat flux for two-phase propane in the vertical HBHX shell side, probably arising from the influence of convection heat transfer in the liquid film. The heat transfer coefficient of two-phase propane in the vertical HBHX is 15–55% lower than that in the horizontal one, due to the thicker liquid film on tubes in the vertical HBHX. A correlation was developed to predict the heat transfer coefficient, and it agrees with 93% of experimental data within a deviation of ±20%.

As a research hotspot, circular RNAs (circRNAs) is one type of non-coding RNAs which have many different functions in biological processes. However, there is lack of study investigating the underlying molecular mechanism and the potential roles of circRNAs in Wilms tumor. We conducted a high-throughput microarray sequencing to screen differentially expressed circRNAs in Wilms tumor. A novel circRNA (circ0093740) was identified as a frequently upregulated circRNA in Wilms tumor cells and tissues. Suppression of circ0093740 remarkably inhibited the proliferation and migration ability in Wilms tumor, validated by several experiments. The molecular mechanism of circ0093740 was investigated by luciferase assays and RNA immunoprecipitation assays. The results revealed that circ0093740 promotes the growth and migration ability by sponging miR-136/145 and upregulating DNMT3A. In conclusion, our study discovered the biological role of the circ0093740-miR-136/145-DNMT3A axis in Wilms tumor growth and metastasis which is important for developing new treatment strategy.

The diversity of waterbirds is threatened, and haemosporidian parasite infection is considered one of the most important causative factors. However, to date, only a few studies focusing on specific parasite species have been carried out, which cannot reflect the general patterns at the community level. To test whether the reported haemosporidian diversity in waterbirds is underestimated, we estimated the prevalence and lineage diversity of avian haemosporidian parasites in 353 waterbirds from 26 species in the Tumuji National Nature Reserve, Northeast China, as well as the host-parasite associations. According to the molecular analysis of cytochrome b (cyt b) barcode sequences, 28.3% of the birds were infected by 49 distinct parasite lineages, including 11 Plasmodium, 12 Haemoproteus, and 26 Leucocytozoon lineages, of which 39 were novel. The highest prevalence was contributed by Leucocytozoon (13.31%), followed by Plasmodium (13.03%) and Haemoproteus (4.25%), which suggested that waterbirds were infected to a lesser extent by Haemoproteus than by the other two genera. Among the most sampled birds, species belonging to Anatidae appeared to be susceptible to Leucocytozoon but resistant to Plasmodium, while Rallidae presented the opposite pattern. On the phylogenetic tree, most of the Leucocytozoon lineages detected in Anatidae clustered together and formed two well-supported clades, while lineages restricted to Gruidae were distantly related to other parasites in all three genera. SW5 was the most abundant lineage and therefore might be a major threat to waterbirds; among the hosts, the common coot harboured the highest diversity of parasite lineages and thus could act as a reservoir for potential transmission. This is the first study of avian haemosporidian infections in a wild waterbird community in Asia. Our findings have doubled the number of lineages recorded in waterbirds, broadened our understanding of host-parasite associations, and addressed the importance of studying haemosporidian infections in wild waterbird conservation., Graphical abstract Image 1, Highlights • The first study of avian haemosporidian infections in a wild waterbird community in Asia. • 49 lineages were detected, nearly 80% were novel. A few lineages were responsible for majority infections. • Prevalence of Plasmodium and Leucocytozoon differ among families, while Haemoproteus is generally low. • Lineages detected in Anatidae and Gruidae were highly specific and phylogenetically distinct.

Conduct disorder (CD) is a psychiatric condition characterized by severe aggressive and antisocial behaviors. Prior neuroimaging work reported that CD is associated with abnormal resting-state local intrinsic brain activity (IBA). However, few studies detected the time-varying brain activity patterns in CD. In this study, eighteen adolescent patients with CD and 18 typically developing controls underwent resting-state functional magnetic resonance imaging scans. We then compared the dynamic characteristics of IBA by calculating the dynamic regional homogeneity (dReHo) through a sliding-window approach between the two groups, and the correlations between the dReHo variability and clinical symptoms in CD were further examined. Moreover, the statistical between-group differences in dReHo were selected as classification features to help distinguish CD patients from controls by adopting a linear support vector machine (SVM) classifier. CD patients showed increased dReHo variability in the left precuneus, right postcentral gyrus, right precentral gyrus, left middle cingulate gyrus, and left paracentral lobule compared to controls, and dReHo variability in the left precuneus was significantly positively associated with impulsiveness scores in CD patients. Importantly, SVM combined with the leave-one-out cross-validation method results demonstrated that 75% (p 0.001) subjects were correctly classified with sensitivity of 61% and specificity of 89%. Our results provided the initial evidence that CD is characterized by abnormal dynamic IBA patterns, giving novel insights into the neuropathological mechanisms of CD. Further, our findings exhibited that the dReHo variability may distinguish CD patients from controls with high accuracy.

The design and synthesis of low-cost and efficient bifunctional electrocatalysts for water splitting are critical and challenging. Hereby, a bimetallic phosphide embedded in a N and P co-doped porous carbon (FeCoP

The SiO2/WO3/NiWO4 composites modified carbon nanofibers (SiWNi-CNFs) were prepared by a facile electrospinning method with following carbonization process under nitrogen atmosphere. The as-obtained SiWNi-CNFs were characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), X-ray photoelectron spectra (XPS), X-ray powder diffraction (XRD), FT-IR spectroscopy and Raman spectroscopy. As revealed by the electrochemical measurement, the SiWNi-CNFs prepared with SiW12/NiAc2 molar ratio of 1:1 presented best hydrogen evolution activity with a small Tafel slope (48 mV dec−1) among all the as-prepared samples. Notably, the as-prepared catalysts exhibit a small onset potential (0.29 V vs. reversible hydrogen electrode), high current density and excellent stability. The experimental results pointed that the SiWNi-CNFs processes more efficient hydrogen evolution properties than that other contrast samples. This is due to the SiO2/WO3/NiWO4 composite modified on the surface of carbon nanofibers can generate numerous active sites from the synergistic effect of each component. At the same time, the intimate combination of ternary oxide and carbon nanofibers can accelerate the electron transfer, enhance the stability and hinder the aggregation of active components during the carbonization. Moreover, the net-like structure stacked by carbon nanofibers should render the exposure of active sites and facilitate the mass transport for the HER process.

Fluorescent silicon nanoparticles (SiNPs) have been proven to be promising materials for diagnosis and therapy of diseases. As the drug carriers and markers in imaging, fluorescent SiNPs trans-membrane is the first step. However, the details of the trans-membrane transport mechanism of SiNPs are still unclear. Herein, we aim to investigate the dynamic trans-membrane transport process of single SiNPs in normal cells (Vero) and cancer cells (HeLa) by utilizing the force tracing technique based on atomic force microscopy.

The component proportion of ethane/propane mixture varies during the condensation process in shell side of helically baffled shell-and-tube heat exchangers of nature gas liquefaction plants, which significantly influences the heat transfer characteristics. In the present study, the heat transfer coefficients of ethane/propane mixtures with ethane mass fraction of 8–50% (covering practical conditions) were tested, and were compared with those of pure propane (0% ethane mass fraction) to analyze the influence of component proportion. The research results indicate that, the maximum heat transfer coefficient of ethane/propane mixture occurs at vapor quality around 0.8; as the ethane mass fraction increases from 0% to 15%, the heat transfer coefficient decreases dramatically by a maximum of 66.3%; as the ethane mass fraction further increases from 15% to 50%, the heat transfer coefficient keeps almost constant, and the fluctuation is within −26.4% to 29.1%. A new heat transfer correlation has been developed, and its prediction deviations are within ±25%.

Carbon dots (CDs) evoke a great deal of attention in biomedicine because of their unique properties. As an emerging theranostic agent, the CDs transmembrane transport process is the first and the most important step. Herein, a transmembrane dynamic process of transporting single CDs with folic acid into normal cells (Vero) and cancer cells (HeLa) were tracked by means of a force tracing technique based on atomic force microscopy. Meanwhile, the kinetic parameters of the the transmembrane process were measured and calculated. Interestingly, in the comparison between cancer cells (HeLa) and normal cells (Vero), the transporting force of single CDs with folic acid was smaller and the transporting average speed was slower.

Background Accurate quantification of infection intensity is essential to estimate infection patterns of avian haemosporidian parasites in order to understand the evolution of host-parasite associations. Traditional microscopy is cost-effective but requires high-quality blood smears and considerable experience, while the widely used semi-quantitative qPCR methods are mostly employed with ideal, laboratory-based golden samples and standard curves, which may limit the comparison of parasitemia from different laboratories. Methods Here we present a digital droplet PCR (ddPCR) protocol for absolute quantification of avian haemosporidians in raptors. Novel primers were designed that target a conserved fragment of a rRNA region of the mitochondrial genome of the parasites. Sensitivity and repeatability were evaluated compared to qPCR and other assays. Results This ddPCR assay enables accurate quantification of haemosporidian parasites belonging to Plasmodium, Haemoproteus and Leucocytozoon with minimum infection quantities of 10−5 (i.e. one parasite copy in 105 host genomes) without the use of standard curves. Quantities assessed by ddPCR were more accurate than qPCR using the same primers through reduction of non-specific amplification in low-intensity samples. The ddPCR technique was more consistent among technical duplicates and reactions, especially when infection intensities were low, and this technique demonstrated equal sensitivity with high correspondence (R2 = 0.97) compared to the widely used qPCR assay. Both ddPCR and qPCR identified more positive samples than the standard nested PCR protocol for the cytb gene used for barcoding avian haemosporidians. Conclusions We developed a novel ddPCR assay enabling accurate quantification of avian haemosporidians without golden samples or standard curves. This assay can be used as a robust method for investigating infection patterns in different host-parasite assemblages and can facilitate the comparison of results from different laboratories.

A novel vital sign detection algorithm based on trajectory capture and peak capture is proposed to solve the problem that the heartbeat signal of slightly moving subjects is easily interfered by breathing harmonics and other clutter. First, the received signal is processed by the average cancellation method to suppress the stationary clutter. Then, we use the trajectory capture algorithm to catch the moving trajectory of the subject, use the trajectory to correct each received pulse, and use the distance gate selection algorithm to extract the body surface vibration signal. After that, the body surface vibration signal was subjected to low-pass filtering in the frequency domain and autocorrelation processing to improve the signal-to-noise ratio of the signal. Finally, the frequency-domain peak capture algorithm of N iterations was used to extract the vital signs of the signal. Simulation results show that the algorithm can accurately extract the vital signs of slightly moving subjects, and has higher measurement accuracy and better stability than the traditional algorithm.

An effectual and understandable route for the fabrication techniques of stereoscopic NO2 sensor is provided in this work. As the gas-sensing layer of the sensor, copper phthalocyanine (CuPc) grew on the top of poly(vinyl alcohol) (PVA) nanofibers (NFs). The sensitivity of the CuPc/PVA NFs stereoscopic sensors to NO2 was over 829%/ppm, while the sensitivity of the continuous CuPc films sensors was 2 orders of magnitude lower than that of the stereoscopic ones. To the responsivities at 25 ppm of NO2, the CuPc/PVA NFs stereoscopic sensors were about four times stronger than that of the continuous CuPc films sensors. For the recovery time, the CuPc/PVA NFs stereoscopic sensors were over eight times faster than the continuous CuPc films sensors. This general tactic can be used to prepare various toxic gas sensors to improve the overall performance of the devices.

Functional neuroimaging studies evaluating sex differences in language processing have been previously reported. However, it remains largely unclear whether there are structural bases for language comprehension and whether these are partially affected by sexual dimorphism in cortical thickness. To this end, we performed correlation analysis between cortical thickness and language comprehension in a large (N = 1017, 549 females, 468 males) young and healthy subjects from Human Connectome Project, with a specific focus on the impact of sex. We identified significant relationship between cortical thickness of the posterior cingulate cortex (PCC) and vocabulary comprehension in females (r = 0.318, r = 10%), while the association was significantly reduced in males (P = 0.017, Cohen's q = 0.154). Furthermore, thickness difference in the PCC was observed to be smaller in females (P < 0.0001, t = -7.12, Cohen's d = 0.45); however, the difference disappeared when controlling for brain size (Cohen's d = 0.002). Our findings indicated that variability in cortical thickness may affect cognitive function much more in females than in males, and highlighted the importance of brain size in explaining sex-specific cortical thickness.

Additional file 3: Figure S2. Output of ddPCR. The sample with high haemosporidian quantity (A03), low quantity (D03), and the non-template control (C10) are presented. a Distribution pattern of droplets; the raindrop function close to the threshold line represents false positives caused by primer dimer or non-specific amplifications. b Histogram of droplets. c Total count of droplets in each PCR reaction well. d Concentration of target gene fragments in each assessed sample, calculated with the default setting of the droplet reader.

For designing helically baffled shell-and-tube heat exchangers (HBHXs) used liquefied natural gas (LNG) process, the condensation heat transfer mechanism in the shell side of HBHXs should be known. In the present study, the condensation heat transfer characteristics and flow patterns of propane spirally flowing in shell side of horizontal HBHXs were experimentally investigated. Experimental conditions cover mass flux of 20–40 kg (m2 s)−1, heat flux of 3.0–7.0 kW m−2, and vapor quality from 0.1 to superheated. The results show that, the heat transfer coefficient decreases with the increase of heat flux, and it initially increases and then decreases with the increase of vapor quality. As the vapor quality increases, the stratified flow, stratified-spray flow and spray flow were observed in sequence. A correlation was developed to predict the heat transfer coefficient, and it can agree with 86% of experimental data within a deviation of ±10%.