57 results on '"Hajime Ishiguro"'
Search Results
2. Genetic manipulation resulting in decreased donor chondroitin sulfate synthesis mitigates hepatic GVHD via suppression of T cell activity
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Suguru Tamura, Hajime Ishiguro, Tatsuya Suwabe, Takayuki Katagiri, Kaori Cho, Kyoko Fuse, Yasuhiko Shibasaki, Tadahisa Mikami, Takero Shindo, Hiroshi Kitagawa, Michihiro Igarashi, Hirohito Sone, Masayoshi Masuko, and Takashi Ushiki
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Medicine ,Science - Abstract
Abstract Donor T cell activation, proliferation, differentiation, and migration are the major steps involved in graft-versus-host disease (GVHD) development following bone marrow transplantation. Chondroitin sulfate (CS) proteoglycan is a major component of the extracellular matrix and causes immune modulation by interacting with cell growth factors and inducing cell adhesion. However, its precise effects on immune function are unclear than those of other proteoglycan families. Thus, we investigated the significance of CS within donor cells in acute GVHD development utilizing CSGalNAc T1-knockout (T1KO) mice. To determine the effects of T1KO, the mice underwent allogenic bone marrow transplantation from major histocompatibility complex-mismatched donors. While transplantation resulted in hepatic GVHD with inflammatory cell infiltration of both CD4+ and CD8+ effector memory T cells, transplantation in T1KO-donors showed milder cell infiltration and improved survival with fewer splenic effector T cells. In vitro T-cell analyses showed that the ratio of effector memory T cells was significantly lower via phorbol myristate acetate/ionomycin stimulation. Moreover, quantitative PCR analyses showed significantly less production of inflammatory cytokines, such as IFN-γ and CCL-2, in splenocytes of T1KO mice. These results suggest that reduction of CS in donor blood cells may suppress the severity of acute GVHD after hematopoietic stem cell transplantation.
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- 2023
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3. Strategic analysis of body composition indices and resting platelet ATP levels in professional soccer players for better platelet-rich plasma therapy
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Takashi Ushiki, Tomoharu Mochizuki, Katsuya Suzuki, Masami Kamimura, Hajime Ishiguro, Tatsuya Suwabe, Satoshi Watanabe, Go Omori, Noriaki Yamamoto, and Tomoyuki Kawase
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soccer player ,sedentary ,platelet ,ATP ,body composition ,basal metabolic rate ,Biotechnology ,TP248.13-248.65 - Abstract
Background: Autologous platelet-rich plasma (PRP) therapy is ambiguously thought to be more effective in elite athletes than in sedentary patients, although the possible importance of recipient responsiveness remains poorly understood. To address this issue, along with the well-known PRP quality, in this initial study, we evaluated two candidate biomarkers: body composition indices (BCIs), which reflect systemic physical conditions, and resting platelet ATP levels, which reflect platelet energy expenditure and the mass of energy generation units.Methods: In this cross-sectional cohort study, blood samples were collected from male professional soccer players (PSPs) on a local professional team during the off-season and platelet ATP levels were quantified using an ATP luminescence assay kit. BCIs were measured using the body mass impedance method. Age-matched male sedentary participants were used as the controls.Results: Among the BCIs, the body mass index, basal metabolic rate (BMR), and skeletal muscle weight levels were higher in the PSPs than in the controls. The platelet ATP levels in the PSPs group were significantly lower than those in the control group. The correlation between BMR and platelet ATP levels was moderately negative in the control group, but weakly positive in the PSPs group.Conclusion: Owing to regular physical exercise, PSPs had higher BMR levels and lower platelet ATP levels without a significant mutual correlation compared to sedentary controls. This study did not indicate the influence of these biomarkers on the success of PRP therapy but provided evidence for a better understanding of PRP therapy, particularly for elite athletes.
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- 2023
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4. Elevated IL-1β and Comparable IL-1 Receptor Antagonist Levels Are Characteristic Features of L-PRP in Female College Athletes Compared to Male Professional Soccer Players
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Tomoharu Mochizuki, Takashi Ushiki, Katsuya Suzuki, Misato Sato, Hajime Ishiguro, Tatsuya Suwabe, Satoshi Watanabe, Mutsuaki Edama, Go Omori, Noriaki Yamamoto, and Tomoyuki Kawase
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platelet-rich plasma ,athletes ,interleukin-1β ,interleukin-1 receptor antagonist ,growth factors ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Autologous platelet-rich plasma (PRP) therapy has been becoming popular for the treatment of musculotendinous injuries among athletes. However, for individual and practical variations, clinical success is hardly predictable. To overcome this difficulty, we have been exploring possible criterion candidates for monitoring its clinical effectiveness. In this study, we focused on sex-based differences in young elite athletes and compared the biochemical compositions of their PRP. Leukocyte-rich PRP (L-PRP) was manually prepared from blood samples collected from male professional soccer players (mPSPs) (n = 25) and female college athletes (fCAs) (n = 36). Platelet-derived growth factor-BB (PDGF-BB), transforming-growth factor-β1 (TGFβ1), platelet factor-4 (PF4), interleukin-1β (IL-1β), and IL-1 receptor antagonist (IL-1RA) were quantified using an enzyme-linked immunosorbent assay. The levels of PDGF-BB, TGFβ1, and PF4 in L-PRP were significantly higher in mPSPs than in fCAs. Conversely, IL-1β and IL-1RA were detected at significantly and slightly higher levels, respectively, in fCAs than in mPSPs. Our findings suggest that, even though L-PRP from fCAs may have lower potential to induce cell growth and differentiation than that of mPSPs, due to the latter’s higher capacity to control inflammation, it does not necessarily imply that PRP treatment in fCAs is less effective. Thus, these cytokine levels should be checked before PRP therapy.
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- 2023
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5. Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels
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Tomoharu Mochizuki, Takashi Ushiki, Katsuya Suzuki, Misato Sato, Hajime Ishiguro, Tatsuya Suwabe, Mutsuaki Edama, Go Omori, Noriaki Yamamoto, and Tomoyuki Kawase
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leukocyte- and platelet-rich plasma ,female college athletes ,PDGF-BB ,TGFβ1 ,IL-1β ,IL-1RA ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Platelet-rich plasma (PRP) has been increasingly used in sports medicine owing to its various advantages. The purpose of our project was to standardize the parameters before performing large-scale clinical trials in the near future to precisely evaluate individual PRP quality. To examine the effects of regular exercise on PRP quality, this study focused on young female athletes, who have been relatively less studied. Blood samples were obtained from female college athletes (n = 35) and ordinary healthy adults (n = 30), which were considered as controls, and leukocyte-rich PRP (L-PRP) was prepared manually. Body composition indices were determined using a bathroom weight scale equipped with an impedance meter. Growth factors and cytokines were quantified using ELISA kits. Platelet-derived growth factor-BB (PDGF-BB) and Transforming-growth factors β1 (TGFβ1) levels (per platelet) in L-PRP were significantly lower in female athletes than in controls. In contrast, Interleukin-1β and Interleukin 1 receptor antagonist (IL-1RA) levels (per platelet and L-PRP) in L-PRP were significantly higher in athletes, and this difference was more prominent in IL-1RA. These findings suggest that L-PRP from athletes may facilitate the inflammatory phase of the healing process by regulating the pro-inflammatory and anti-inflammatory balance. These chemical compositions can be adopted as “must-check” parameters to characterize individual PRP preparations prior to clinical trials.
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- 2023
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6. Platelet polyphosphate and energy metabolism in professional male athletes (soccer players): A cross‐sectional pilot study
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Takashi Ushiki, Tomoharu Mochizuki, Katsuya Suzuki, Masami Kamimura, Hajime Ishiguro, Satoshi Watanabe, Go Omori, Noriaki Yamamoto, and Tomoyuki Kawase
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athletes ,basal metabolic rate ,NADH ,platelets ,polyphosphates ,Physiology ,QP1-981 - Abstract
Abstract Human platelet polyphosphate (polyP) is a multifunctional molecule; however, its functions are not yet fully understood. A recent study demonstrated that similar to skeletal muscle, polyP is involved in energy metabolism in platelets, which suggests that well‐trained athletes may exhibit elevated platelet polyP levels for energy storage. To test this hypothesis, we quantified platelet polyP along with NADH, a component involved in ATP production in non‐trained and well‐trained male Japanese participants of the same generation. Washed platelets were prepared from the venous blood of young, healthy, non‐athletes, and professional soccer players (pro‐athletes). NADH and polyP levels were spectrophotometrically determined using tetrazolium reduction and fluorometrically determined using 4′,6‐diamidino‐2‐phenylindole at the excitation/emission wavelengths of 425/525 nm. Body weight and impedances were measured simultaneously. Statistical analyses were performed using the Mann–Whitney U test and Spearman correlation coefficient. Although basal metabolic rate levels were significantly higher, platelet polyP levels were significantly lower in pro‐athletes than in that in non‐athletes. No significant differences were detected in other body compositions or platelet indices between the two groups. The pro‐athlete group showed a moderate, nearly significant correlation (R = 0.439; p = 0.0512) between platelet polyP and NADH levels. Taken together with the weak correlation data between polyP and body mass index, it is suggested that platelet polyP levels may be influenced by platelet and body energy metabolic activity. Further biochemical studies are needed to elucidate this mechanism.
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- 2022
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7. Altered microbiota by a high-fat diet accelerates lethal myeloid hematopoiesis associated with systemic SOCS3 deficiency
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Kaori Cho, Takashi Ushiki, Hajime Ishiguro, Suguru Tamura, Masaya Araki, Tatsuya Suwabe, Takayuki Katagiri, Mari Watanabe, Yoko Fujimoto, Riuko Ohashi, Yoichi Ajioka, Ippei Shimizu, Shujiro Okuda, Masayoshi Masuko, Yoshimi Nakagawa, Hideyo Hirai, Warren S. Alexander, Hitoshi Shimano, and Hirohito Sone
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immunology ,microbiome ,Science - Abstract
Summary: The suppressors of cytokine signaling (SOCS) proteins are negative regulators of cytokine signaling required to prevent excessive cellular responses. In particular, SOCS3 is involved in the regulation of metabolic syndromes, such as obesity and diabetes, by suppressing leptin and insulin signals. SOCS3 also suppresses the inflammatory response associated with metabolic stress, but this specific role remains undefined. Wild-type mice on a high-fat diet (HFD) exhibited only fatty liver, whereas systemic deletion of SOCS3 resulted in excessive myeloid hematopoiesis and hepatic inflammation. In addition, depletion of the gut microbiota resulted in considerable improvement in excess granulopoiesis and splenomegaly, halting the progression of systemic inflammation in SOCS3KO mice on the HFD. This result suggests that intestinal dysbiosis is involved in inflammation associated with SOCS3KO. Although contributing to diet-induced obesity and fatty liver, SOCS3 is nevertheless critical to suppress excess myeloid hematopoiesis and severe systemic inflammation associated with intestinal dysbiosis on HFD.
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- 2021
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8. Modulation of ATP Production Influences Inorganic Polyphosphate Levels in Non-Athletes’ Platelets at the Resting State
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Takashi Ushiki, Tomoharu Mochizuki, Katsuya Suzuki, Masami Kamimura, Hajime Ishiguro, Tatsuya Suwabe, and Tomoyuki Kawase
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platelet ,polyphosphate ,ATP ,NADH ,oxidative phosphorylation ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Platelets produce inorganic polyphosphate (polyP) upon activation to stimulate blood coagulation. Some researchers have linked polyP metabolism to ATP production, although the metabolic linkage is yet to be elucidated. We found evidence for this possibility in our previous study on professional athletes (versus non-athletes), and proposed that the regulatory mechanism might be different for these two groups. To explore this aspect further, we investigated the effects of modulated ATP production on polyP levels. Blood samples were obtained from Japanese healthy, non-athletes in the presence of acid-citrate-dextrose. The platelets in the plasma were treated with oligomycin, rotenone, and GlutaMAX to modulate ATP production. PolyP level was quantified fluorometrically and visualized using 4′,6-diamidino-2-phenylindole. Correlations between polyP and ATP or NADH were then calculated. Contrary to the hypothesis, inhibitors of ATP production increased polyP levels, whereas amino acid supplementation produced the opposite effect. In general, however, polyP levels were positively correlated with ATP levels and negatively correlated with NADH levels. Since platelets are metabolically active, they exhibit high levels of ATP turnover rate. Therefore, these findings suggest that ATP may be involved in polyP production in the resting platelets of non-athletes.
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- 2022
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9. Quantitative Relationship Between Cumulative Risk Alleles Based on Genome-Wide Association Studies and Type 2 Diabetes Mellitus: A Systematic Review and Meta-analysis
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Satoru Kodama, Kazuya Fujihara, Hajime Ishiguro, Chika Horikawa, Nobumasa Ohara, Yoko Yachi, Shiro Tanaka, Hitoshi Shimano, Kiminori Kato, Osamu Hanyu, and Hirohito Sone
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genome-wide association studies ,risk allele ,type 2 diabetes mellitus ,meta-analysis ,Medicine (General) ,R5-920 - Abstract
Many epidemiological studies have assessed the genetic risk of having undiagnosed or of developing type 2 diabetes mellitus (T2DM) using several single nucleotide polymorphisms (SNPs) based on findings of genome-wide association studies (GWAS). However, the quantitative association of cumulative risk alleles (RAs) of such SNPs with T2DM risk has been unclear. The aim of this meta-analysis is to review the strength of the association between cumulative RAs and T2DM risk. Systematic literature searches were conducted for cross-sectional or longitudinal studies that examined odds ratios (ORs) for T2DM in relation to genetic profiles. Logarithm of the estimated OR (log OR) of T2DM for 1 increment in RAs carried (1-ΔRA) in each study was pooled using a random-effects model. There were 46 eligible studies that included 74,880 cases among 249,365 participants. In 32 studies with a cross-sectional design, the pooled OR for T2DM morbidity for 1-ΔRA was 1.16 (95% confidence interval [CI], 1.13–1.19). In 15 studies that had a longitudinal design, the OR for incident T2DM was 1.10 (95% CI, 1.08–1.13). There was large heterogeneity in the magnitude of log OR (P < 0.001 for both cross-sectional studies and longitudinal studies). The top 10 commonly used genes significantly explained the variance in the log OR (P = 0.04 for cross-sectional studies; P = 0.006 for longitudinal studies). The current meta-analysis indicated that carrying 1-ΔRA in T2DM-associated SNPs was associated with a modest risk of prevalent or incident T2DM, although the heterogeneity in the used genes among studies requires us to interpret the results with caution.
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- 2018
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10. Carrot Consumption Frequency Associated with Reduced BMI and Obesity through the SNP Intermediary rs4445711
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Kazuya Fujihara, Shun Nogawa, Kenji Saito, Chika Horikawa, Yasunaga Takeda, Kaori Cho, Hajime Ishiguro, Satoru Kodama, Yoshimi Nakagawa, Takashi Matsuzaka, Hitoshi Shimano, and Hirohito Sone
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carrot intake ,body mass index ,single nucleotide polymorphism ,rs4445711 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
It is unclear whether genetic interactions are involved in the association between vegetable intake and reduced body mass index (BMI) or obesity. We conducted a comprehensive search for single nucleotide polymorphisms (SNPs) which are associated with the interaction between vegetable intake frequency and BMI or obesity. We performed a genome-wide association analysis to evaluate the genetic interactions between self-reported intake of vegetables such as carrot, broccoli, spinach, other green vegetables (green pepper and green beans), pumpkin, and cabbage with BMI and obesity, which is defined as a BMI ≥ 25.0 kg/m2 in the Japanese population (n = 12,225). The mean BMI and prevalence of obesity was 23.9 ± 3.4 kg/m2 and 32.3% in men and 22.1 ± 3.8 kg/m2 and 17.3% in in women, respectively. A significant interaction was observed between rs4445711 and frequency of carrot intake on BMI (p = 4.5 × 10−8). This interaction was slightly attenuated after adjustment for age, sex, alcohol intake, smoking, physical activity and the frequency of total vegetable intake (p = 2.1 × 10−7). A significant interaction was also observed between rs4445711 and frequency of carrot intake on obesity (p = 2.5 × 10−8). No significant interactions that were the same as the interaction between frequency of carrot intake and rs4445711 were observed between the intake frequency of broccoli, spinach, other green vegetables, pumpkin or cabbage and BMI or obesity. The frequency of carrot consumption is implicated in reducing BMI by the intermediary of rs4445711. This novel genetic association may provide new clues to clarify the association between vegetable intake and BMI or obesity.
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- 2021
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11. Pulse Pressure is a Stronger Predictor Than Systolic Blood Pressure for Severe Eye Diseases in Diabetes Mellitus
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Masahiko Yamamoto, Kazuya Fujihara, Masahiro Ishizawa, Taeko Osawa, Masanori Kaneko, Hajime Ishiguro, Yasuhiro Matsubayashi, Hiroyasu Seida, Nauta Yamanaka, Shiro Tanaka, Satoru Kodama, Hiruma Hasebe, and Hirohito Sone
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blood pressure ,diabetic mellitus ,hemoglobin A1c ,pulse pressure ,systolic blood pressure ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background Evidence of the role of systolic blood pressure (SBP) in development of severe diabetic retinopathy is not strong, although the adverse effect of low diastolic blood pressure has been a partial explanation. We assessed the predictive ability of incident severe diabetic retinopathy between pulse pressure (PP) which considers both SBP and diastolic blood pressure, compared with SBP. Methods and Results Eligible patients (12 242, 83% men) aged 19 to 72 years from a nationwide claims database were analyzed for a median observational 4.8‐year period. Severe diabetic retinopathy was defined as vision‐threatening treatment‐required diabetic eye diseases. Multivariate Cox regression analysis revealed that hazard ratios (95% CI) of treatment‐required diabetic eye diseases for 1 increment of standard deviation and the top tertile compared with the bottom tertile were 1.39 (1.21–1.60) and 1.72 (1.17–2.51), respectively, for PP and 1.22 (1.05–1.41) and 1.43 (0.97–2.11), respectively, for SBP adjusted for age, sex, body mass index, hemoglobin A1c, fasting plasma glucose, lipids, and smoking status. In a model with SBP and PP simultaneously as covariates, the hazard ratios of only PP (hazard ratios [95% CI], 1.57 [1.26–1.96]) but not SBP (0.85 [0.68–1.07]) were statistically significant. Delong test revealed a significant difference in the area under the receiver operating characteristic curve between PP and SBP (area under the receiver operating characteristic curve [95% CI], 0.58 [0.54–0.63] versus 0.54 [0.50–0.59]; P=0.03). The strongest predictor remained as hemoglobin A1c (area under the receiver operating characteristic curve [95% CI], 0.80 [0.77–0.84]). Conclusions After excluding the significant impact of glycemic control, PP in comparison with SBP is a better predictor of severe diabetic retinopathy, suggesting a role of diastolic blood pressure and arterial stiffness in pathology.
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- 2019
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12. Osteoclastogenic Potential of Tissue-Engineered Periosteal Sheet: Effects of Culture Media on the Ability to Recruit Osteoclast Precursors
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Kohya Uematsu, Takashi Ushiki, Hajime Ishiguro, Riuko Ohashi, Suguru Tamura, Mari Watanabe, Yoko Fujimoto, Masaki Nagata, Yoichi Ajioka, and Tomoyuki Kawase
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periosteal sheets ,green fluorescent protein ,bone marrow transplantation ,CD11b ,osteoclastogenesis ,tartrate-resistant acid phosphatase ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Cell culture media influence the characteristics of human osteogenic periosteal sheets. We have previously found that a stem cell medium facilitates growth and collagen matrix formation in vitro and osteogenesis in vivo. However, it has not yet been demonstrated which culture medium is superior for osteoclastogenesis, a prerequisite for reconstruction of normal bone metabolic basis. To address this question, we compared chemotaxis and osteoclastogenesis in tissue-engineered periosteal sheets (TPSs) prepared with two types of culture media. Periosteal tissues obtained from adult volunteers were expanded with the conventional Medium 199 or with the stem cell medium, MesenPRO. Hematopoietic enhanced-green-fluorescent-protein (EGFP)-nude mice were prepared by γ-irradiation of Balb/c nu/nu mice and subsequent transplantation of bone marrow cells from CAG-EGFP C57BL/6 mice. TPSs were implanted subcutaneously into the chimeric mice and retrieved after intervals for immunohistopathological examination. EGFP+ cells were similarly recruited to the implantation site in both the TPSs prepared, whereas the distribution of CD11b+ cells was significantly lower in the TPS prepared with the stem cell medium. Instead, osteoclastogenesis was higher in the TPS prepared with the stem cell medium than in the one prepared with the conventional medium. These findings suggest that the stem cell medium is preferable for the preparation of more functional TPSs.
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- 2021
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13. Network meta-analysis of glucose-lowering drug treatment regimens with the potential risk of hypoglycemia in patients with type 2 diabetes mellitus in terms of glycemic control and severe hypoglycemia
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Satoru Kodama, Kazuya Fujihara, Hajime Ishiguro, Yasuhiro Matsubayashi, Masaru Kitazawa, Midori Iwanaga, Takaho Yamada, Kiminori Kato, Yoshimi Nakagawa, Shiro Tanaka, Hitoshi Shimano, and Hirohito Sone
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General Medicine ,General Biochemistry, Genetics and Molecular Biology - Abstract
Insulin and its secretagogues are essential for some patients with type 2 diabetes (T2D) to maintain good glycemic control (GC), but severe hypoglycemia (SH) is a concern. This network meta-analysis aimed to find optimal glucose-lowering drug treatment regimens in terms of GC and SH in T2D patients. MEDLINE and EMBASE were used to identify trials that compared two or more treatments including insulins and/or sulfonylurea or glinides and that examined both GC and SH. Treatment hierarchy was expressed as the surface under the cumulative ranking curve (SUCRA) probabilities. We identified 137 eligible trials comprising 42 treatments. The use of insulins and non-insulin glucose-lowering agents except for sulfonylurea or glinide had a higher SUCRA than insulins only for hemoglobin A1c (A1C) (p = 0.01) changes and achievement of A1C
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- 2023
14. Utility of a Physical Fitness Score in Screening for Chronic Diseases
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Takaaki Sato, Kazuya Fujihara, Mayuko Harada Yamada, Kaori Chou, Yuta Yaguchi, Masaru Kitazawa, Hajime Ishiguro, Taeko Osawa, Takaho Yamada, Satoru Kodama, Kiminori Kato, and Hirohito Sone
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Physical Therapy, Sports Therapy and Rehabilitation ,Orthopedics and Sports Medicine ,Research Article - Abstract
We developed a new Physical Score (PS) consisting of comprehensive physical fitness indicators and elucidated the association between the resultant PS and metabolic diseases, i.e., diabetes, hypertension, dyslipidemia, fatty liver, and metabolic syndrome (MetS), among Japanese. Analyzed were 49,850 persons (30,039 men) aged 30 to 69 y who underwent physical fitness tests. Principal component analysis was performed on the correlation matrix of the physical fitness test results (relative grip strength, single-leg balance with eyes closed, and forward bending) according to sex and age. We defined the PS as the first principal component score. A formula was developed for various age groups comprised of men and women from 30 to 69 years of age from which the PS for each age and sex was calculated. The PS for both men and women was normally distributed with a value of 0 ± 1.15-1.16. Multivariate logistic regression analysis showed that the risk of metabolic diseases increased approximately 1.1-1.6 times per each 1-point reduction in the PS. The association between PS and MetS was particularly strong in that a 1-point reduction in the PS increased the risk of MetS by 1.54 times (95% confidence interval 1.46 to 1.62) in men and by 1.21 times (1.15 to 1.28) in women. The association between a lower PS and disease risk was stronger in younger men for fatty liver and in older men for MetS. Conversely, in women, the association between a lower PS and disease risk was stronger in older women for fatty liver and in younger women for MetS. For diabetes, hypertension, and dyslipidemia, the change in the impact of PS reductions across age groups was small. The PS is a useful and simple non-invasive tool for screening Japanese people for metabolic diseases.
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- 2023
15. 472-P: Sex Differences in the Effect of High Serum Uric Acid on the Incidence of Vision-Threatening Retinopathy in Those with Type 2 Diabetes
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MASAHIKO YAMAMOTO, KAZUYA FUJIHARA, HAJIME ISHIGURO, YUTA YAGUCHI, MASAHIRO ISHIZAWA, MAYUKO H. YAMADA, TAEKO OSAWA, MASARU KITAZAWA, MASANORI KANEKO, YASUHIRO MATSUBAYASHI, MIDORI IWANAGA, TAKAHO YAMADA, SATORU KODAMA, and HIROHITO SONE
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Endocrinology, Diabetes and Metabolism ,Internal Medicine - Abstract
Elevated serum uric acid reportedly increases the risk of development and progression of diabetic retinopathy with conflicting results. However, whether it also increases the incidence of severe vision-threatening diabetic eye diseases has been scarcely investigated. Also unknown is whether there are sex differences in the incidence of advanced retinopathies. To clarify this, we utilized ICD-and medical treatment codes from a nationwide claims database that finally included 13,721 type 2 diabetes patients (mean age 50 y, HbA1c 7.0%, mean follow-up period 5.8 y, 10,962 men) . During the study period, 388 patients developed vision-threatening treatment-required diabetic eye diseases (4.9/1000 person-years) . Multivariate Cox analysis demonstrated that a serum uric acid ≥7.1 mg/dl was significantly positively associated in men with future occurrence of treatment-required diabetic eye diseases, but not in women. Hazard ratios for those with serum uric acid ≥7.1 mg/dl for incident treatment-required diabetic eye diseases were 1.36 (95% confidence interval, 1.01-1.85) in men and 2. (0.71-6.08) in women, compared with the reference group ( Disclosure M. Yamamoto: None. K. Fujihara: None. H. Ishiguro: None. M. Ishizawa: None. M.H. Yamada: None. T. Osawa: None. M. Kitazawa: None. M. Kaneko: None. M. Iwanaga: None. T. Yamada: None. S. Kodama: None. H. Sone: Research Support; Astellas Pharma Inc., Eisai Co., Ltd., Kyowa Kirin Co., Ltd., Novo Nordisk, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Holdings Co., Ltd., Takeda Pharmaceutical Company Limited. Funding The Japan Society for the Promotion of Science and the Ministry of Health, Labor and Welfare, Japan. (18K17897)
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- 2022
16. 539-P: Evaluation of a Comprehensive Physical Fitness Index to Predict Future Prediabetes and Metabolic Syndrome (MetS)
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TAKAAKI SATO, KAZUYA FUJIHARA, MAYUKO H. YAMADA, KAORI CHO, YUTA YAGUCHI, MASAHIKO YAMAMOTO, MASARU KITAZAWA, HAJIME ISHIGURO, TAEKO OSAWA, TAKAHO YAMADA, KIMINORI KATO, and HIROHITO SONE
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Endocrinology, Diabetes and Metabolism ,Internal Medicine - Abstract
Muscle strength, balance, and flexibility has been associated with the development of type 2 diabetes and atherosclerosis, but little is known whether the physical score (PS) , which integrates these physical fitness indices, can predict future metabolic diseases. We investigated the longitudinal relationship between baseline and changes in PS and future prediabetes and MetS. Analyzed were 5620 men aged 30 to 69 y without MetS who underwent physical fitness tests. Principal component analysis was performed on the correlation matrix of the physical fitness test results according to age. PS was defined as the first principal component score. Associations between PS values at the start of observation (year 0) , change in the PS one year later (year 1) and the onset of prediabetes and MetS at the end of observation (years 4 or 5) were examined by Cox hazard regression analysis. The same analysis was performed for the 3812 men without prediabetes at baseline. No significant difference was found for the development of prediabetes, but a significant difference was found for development of MetS. The hazard ratio for the development of MetS was 1.30 (1.18,1.44) for every 1 decrease in the PS in year 0 and 1.35 (1.13,1.60) for every 1 decrease in the change in PS from year 0 to year 1. PS was a simple and non-invasive indicator to predict MetS. Disclosure T. Sato: None. K. Fujihara: None. M. Yamamoto: None. M. Kitazawa: None. H. Ishiguro: None. T. Osawa: None. H. Sone: Research Support; Astellas Pharma Inc., Eisai Co., Ltd., Kyowa Kirin Co., Ltd., Novo Nordisk, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Holdings Co., Ltd., Takeda Pharmaceutical Company Limited.
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- 2022
17. 1091-P: Impact of Metabolic Syndrome and Metabolic Dysfunction-Associated Fatty Liver Disease on Cardiovascular Risk in People with and without Type 2 Diabetes
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YASUHIRO MATSUBAYASHI, KAZUYA FUJIHARA, MAYUKO H. YAMADA, YUTA YAGUCHI, MASAHIKO YAMAMOTO, HAJIME ISHIGURO, MASARU KITAZAWA, MIDORI IWANAGA, TAKAHO YAMADA, SATORU KODAMA, and HIROHITO SONE
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Endocrinology, Diabetes and Metabolism ,Internal Medicine - Abstract
This study aims to examine the impact of the association between metabolic syndrome (MetS) and metabolic dysfunction-associated fatty liver disease (MAFLD) , which both have insulin resistance and are pathophysiologically similar, on the development of new-onset cardiovascular disease, with and without type 2 diabetes mellitus (T2DM) A total of 570,426 participants without a history of cardiovascular disease who were enrolled in the nationwide claims database from 2008-2016 were included in this study. Participants were classified according to the presence or absence of MetS and/or MAFLD, and the risk of developing coronary artery disease (CAD) and/or cerebrovascular disease (CVD) in each category was analyzed using a multivariate Cox proportional hazard model. The same analysis was performed with stratification by the presence or absence of T2DM. During a median follow-up of 5.2 years, 2,252 CAD and 3,128 CVD events occurred. The hazard ratio (HR) (95% CI) for CAD, compared with the group with neither MAFLD nor MetS, was 1.46 (1.32-1.62) for MAFLD only (without MetS) , 2.02 (1.51-2.70) for MetS only (without MAFLD) , and 2.33 (2.10-2. 58) for MAFLD + MetS. For CVD, The HR was 1.41 (1.28-1.55) for MAFLD only, 1.67 (1.38-2.02) for MetS only, and 1.89 (1.72-2.08) for MAFLD + MetS. These results were similar for non-T2DM participants but different for T2DM participants, with no significant increased risk for CAD in MetS only and a significant increased risk for CVD only when both MAFLD and MetS coexisted. The predictive ability for cardiovascular disease development in patients with MetS and MAFLD differed depending on the presence or absence of T2DM. These results suggest that distinguishing between the diagnosis of MetS and MAFLD, taking into account the presence or absence of T2DM, may lead to more accurate identification of patients at high risk of developing cardiovascular disease. Disclosure K. Fujihara: None. M.H. Yamada: None. M. Yamamoto: None. H. Ishiguro: None. M. Kitazawa: None. M. Iwanaga: None. S. Kodama: None. H. Sone: Research Support; Astellas Pharma Inc., Eisai Co., Ltd., Kyowa Kirin Co., Ltd., Novo Nordisk, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Holdings Co., Ltd., Takeda Pharmaceutical Company Limited. Funding The Japan Society for Promotion of Science (JSPS) KAKENHI Grant Number JP 20K19706)
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- 2022
18. 1438-P: Association between Screen Time, Including That for Smartphones, and Overweight/Obesity among Children in Japan
- Author
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IZUMI IKEDA, KAZUYA FUJIHARA, SAKIKO Y. MORIKAWA, YASUNAGA TAKEDA, HAJIME ISHIGURO, MAYUKO H. YAMADA, CHIKA HORIKAWA, YASUHIRO MATSUBAYASHI, TAKAHO YAMADA, YOHEI OGAWA, and HIROHITO SONE
- Subjects
Endocrinology, Diabetes and Metabolism ,Internal Medicine - Abstract
The association between screen time (ST) , including that for smartphones, and overweight/obesity (OW/OB) in children was examined separately for boys and girls, considering the influence of lifestyle factors including diet, physical activity, and sleep time. A cross-sectional study was conducted in 2242 Japanese children (1278 girls) aged 10-14 years. OW/OB was defined by the International Obesity Task Force. Logistic regression analysis showed that only for girls, total ST (≥4h) , smartphone ST (≥3h) and non-smartphone ST (≥2h) were all independently and significantly associated with overweight/obesity compared to In Japanese girls, smartphone ST, non-smartphone ST, and total ST were all significantly associated with OW/OB. To avoid OW/OB, it is suggested to keep smartphone ST, non-smartphone ST, and total ST to Disclosure K. Fujihara: None. S.Y. Morikawa: None. H. Ishiguro: None. M.H. Yamada: None. C. Horikawa: None. Y. Ogawa: None. H. Sone: Research Support; Astellas Pharma Inc., Eisai Co., Ltd., Kyowa Kirin Co., Ltd., Novo Nordisk, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Holdings Co., Ltd., Takeda Pharmaceutical Company Limited.
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- 2022
19. 378-P: Network Meta-analysis of Hypoglycemic Treatment Regimens with Potential Risk of Hypoglycemia in Terms of Glycemic Control and Severe Hypoglycemia
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SATORU KODAMA, TAKAAKI SATO, MAYUKO H. YAMADA, MASAHIKO YAMAMOTO, YASUHIRO MATSUBAYASHI, HAJIME ISHIGURO, MIDORI IWANAGA, KAZUYA FUJIHARA, TAKAHO YAMADA, KIMINORI KATO, KENICHI WATANABE, and HIROHITO SONE
- Subjects
Endocrinology, Diabetes and Metabolism ,Internal Medicine - Abstract
Insulin and its secretagogues are essential for some patients with type 2 diabetes (T2D) to maintain glycemic control (GC) . However, these drugs are inevitably accompanied by hypoglycemia. Avoiding severe hypoglycemia (SH) is essential considering its poor prognosis. This network meta-analysis aimed to find optimal hypoglycemic treatment regimens for T2D in terms of GC and SH. MEDLINE and EMBASE were used to identify trials comparing 2 or more treatments including insulins and/or SU/glinide and included both GC and SH in study outcomes. Treatment hierarchy was summarized as the surface under cumulative ranking probabilities (SUCRA) . There were 137 eligible trials from which 36 treatments were identified. Insulin plus (+) non-insulin drugs except for SU/glinide (others) had a higher SUCRA for changes in hemoglobin A1c (A1C) compared with only insulins (68.2±5.6% vs. 39.9±6.6%; P=0.01) although the difference in the SUCRA for SH was not significant (P=0.59) . Compared with only insulins, SU/glinide + others had a lower SUCRA for SH (42.4±5.8% vs. 58.5±3.4%, P=0.05) and comparable SUCRA for A1C change (P=1.00) . Cluster analysis indicated that premixed insulin + glucagon-like peptide-1 receptor agonist (Mix-ins+GLP1) consistently belonged to the high-efficacy group for A1C change and achievement of A1C Disclosure S.Kodama: None. K.Kato: None. K.Watanabe: None. H.Sone: Research Support; Astellas Pharma Inc., Eisai Co., Ltd., Kyowa Kirin Co., Ltd., Novo Nordisk, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Holdings Co., Ltd., Takeda Pharmaceutical Company Limited. T.Sato: None. M.H.Yamada: None. M.Yamamoto: None. Y.Matsubayashi: None. H.Ishiguro: None. M.Iwanaga: None. K.Fujihara: None. T.Yamada: None.
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- 2022
20. Leisure-time physical activity and incidence of objectively assessed hearing loss: The Niigata Wellness Study
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Hajime Ishiguro, Chika Horikawa, Kiminori Kato, Hirohito Sone, Kazuya Fujihara, Motohiko Miyachi, I-Min Lee, Susumu S. Sawada, Minoru Tashiro, Ryoko Kawakami, Hideaki Oike, Haruki Momma, Yuko Gando, and Yasuhiro Matsubayashi
- Subjects
Adult ,Male ,medicine.medical_specialty ,Hearing loss ,Low Frequency Hearing Loss ,Physical Therapy, Sports Therapy and Rehabilitation ,Audiology ,Cohort Studies ,Leisure Activities ,otorhinolaryngologic diseases ,medicine ,Humans ,Orthopedics and Sports Medicine ,Hearing Loss ,Exercise ,medicine.diagnostic_test ,business.industry ,Incidence (epidemiology) ,Incidence ,Hazard ratio ,Confidence interval ,Cohort ,Female ,Audiometry ,medicine.symptom ,business ,Cohort study - Abstract
Previous cohort study reported that high physical activity was associated with a low risk of self-reported hearing loss in women. However, no studies have examined the association between physical activity and the development of hearing loss as measured using an objective assessment of hearing loss in men and women. Here, we used cohort data to examine the association between leisure-time physical activity and incidence of objectively assessed hearing loss in men and women. Participants included 27 537 Japanese adults aged 20-80 years without hearing loss, who completed a self-administered physical activity questionnaire between April 2001 and March 2002. The participants were followed up for the development of hearing loss as measured by audiometry between April 2002 and March 2008. During follow-up, 3691 participants developed hearing loss. Compared with the none physical activity group, multivariable adjusted hazard ratios (HRs) for developing hearing loss were 0.93 (95% confidence interval (CI), 0.86-1.01) and 0.87 (0.81-0.95) for the medium (
- Published
- 2021
21. Altered microbiota by a high-fat diet accelerates lethal myeloid hematopoiesis associated with systemic SOCS3 deficiency
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Shujiro Okuda, Masaya Araki, Takayuki Katagiri, Suguru Tamura, Yoichi Ajioka, Hajime Ishiguro, Yoshimi Nakagawa, Tatsuya Suwabe, Riuko Ohashi, Hitoshi Shimano, Ippei Shimizu, Hirohito Sone, Mari Watanabe, Yoko Fujimoto, Warren S. Alexander, Takashi Ushiki, Kaori Cho, Masayoshi Masuko, and Hideyo Hirai
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Multidisciplinary ,Myeloid ,biology ,business.industry ,medicine.medical_treatment ,Science ,Fatty liver ,digestive, oral, and skin physiology ,microbiome ,Inflammation ,Gut flora ,biology.organism_classification ,medicine.disease ,Systemic inflammation ,Granulopoiesis ,Article ,immunology ,medicine.anatomical_structure ,Cytokine ,Immunology ,Medicine ,SOCS3 ,medicine.symptom ,business - Abstract
Summary The suppressors of cytokine signaling (SOCS) proteins are negative regulators of cytokine signaling required to prevent excessive cellular responses. In particular, SOCS3 is involved in the regulation of metabolic syndromes, such as obesity and diabetes, by suppressing leptin and insulin signals. SOCS3 also suppresses the inflammatory response associated with metabolic stress, but this specific role remains undefined. Wild-type mice on a high-fat diet (HFD) exhibited only fatty liver, whereas systemic deletion of SOCS3 resulted in excessive myeloid hematopoiesis and hepatic inflammation. In addition, depletion of the gut microbiota resulted in considerable improvement in excess granulopoiesis and splenomegaly, halting the progression of systemic inflammation in SOCS3KO mice on the HFD. This result suggests that intestinal dysbiosis is involved in inflammation associated with SOCS3KO. Although contributing to diet-induced obesity and fatty liver, SOCS3 is nevertheless critical to suppress excess myeloid hematopoiesis and severe systemic inflammation associated with intestinal dysbiosis on HFD., Graphical abstract, Highlights • SOCS3 suppresses severe systemic inflammation associated with high-fat diet • SOCS3 deficiency on high-fat diet accelerates excess myeloid hematopoiesis • SOCS3 controls gut dysbiosis on high-fat diet, Immunology; Microbiome
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- 2021
22. Carrot Consumption Frequency Associated with Reduced BMI and Obesity through the SNP Intermediary rs4445711
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Yoshimi Nakagawa, Yasunaga Takeda, Hitoshi Shimano, Hajime Ishiguro, Shun Nogawa, Satoru Kodama, Kaori Cho, Kazuya Fujihara, Chika Horikawa, Kenji Saito, Hirohito Sone, and Takashi Matsuzaka
- Subjects
Male ,Single-nucleotide polymorphism ,body mass index ,Polymorphism, Single Nucleotide ,Article ,Animal science ,Gene Frequency ,single nucleotide polymorphism ,Green pepper ,Medicine ,SNP ,Humans ,TX341-641 ,Obesity ,Genetic association ,Nutrition and Dietetics ,business.industry ,Genome, Human ,Nutrition. Foods and food supply ,rs4445711 ,food and beverages ,Feeding Behavior ,Japanese population ,Middle Aged ,medicine.disease ,Daucus carota ,carrot intake ,Alcohol intake ,Female ,business ,Body mass index ,Food Science - Abstract
It is unclear whether genetic interactions are involved in the association between vegetable intake and reduced body mass index (BMI) or obesity. We conducted a comprehensive search for single nucleotide polymorphisms (SNPs) which are associated with the interaction between vegetable intake frequency and BMI or obesity. We performed a genome-wide association analysis to evaluate the genetic interactions between self-reported intake of vegetables such as carrot, broccoli, spinach, other green vegetables (green pepper and green beans), pumpkin, and cabbage with BMI and obesity, which is defined as a BMI ≥ 25.0 kg/m2 in the Japanese population (n = 12,225). The mean BMI and prevalence of obesity was 23.9 ± 3.4 kg/m2 and 32.3% in men and 22.1 ± 3.8 kg/m2 and 17.3% in in women, respectively. A significant interaction was observed between rs4445711 and frequency of carrot intake on BMI (p = 4.5 × 10−8). This interaction was slightly attenuated after adjustment for age, sex, alcohol intake, smoking, physical activity and the frequency of total vegetable intake (p = 2.1 × 10−7). A significant interaction was also observed between rs4445711 and frequency of carrot intake on obesity (p = 2.5 × 10−8). No significant interactions that were the same as the interaction between frequency of carrot intake and rs4445711 were observed between the intake frequency of broccoli, spinach, other green vegetables, pumpkin or cabbage and BMI or obesity. The frequency of carrot consumption is implicated in reducing BMI by the intermediary of rs4445711. This novel genetic association may provide new clues to clarify the association between vegetable intake and BMI or obesity.
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- 2021
23. 421-P: Genetically Reduced Chondroitin Sulfate Prevents the Progression of Diabetic Neuropathy via Pericyte Functions
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Takashi Ushiki, Masayoshi Masuko, Atsuko Honda, Michihiro Igarashi, Tadahisa Mikami, Hajime Ishiguro, Kazunori Sango, Hiroshi Kitagawa, and Hirohito Sone
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medicine.medical_specialty ,Diabetic neuropathy ,biology ,business.industry ,Endocrinology, Diabetes and Metabolism ,medicine.disease ,Streptozotocin ,Nerve conduction velocity ,chemistry.chemical_compound ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,biology.protein ,Medicine ,Sciatic nerve ,Pericyte ,Chondroitin sulfate ,business ,Platelet-derived growth factor receptor ,medicine.drug - Abstract
Background: The extracellular matrix is associated with diabetic complications; however, chondroitin sulfate (CS) role remains unclear. Herein, genetically engineered mice lacking the rate-limiting CS-synthesizing enzyme, CS N-acetylgalactosaminyltransferase-1 (T1), were used to clarify CS effects on diabetic neuropathy. Diabetes was induced by streptozotocin injection in 6-week-old male T1 knockout (T1KO) and wild-type (WT) mice. Results: Previously reported preliminary data at the former ADA meeting revealed that thermal nociception and the number of plantar peripheral nerve fibers were disrupted in diabetic WT mice, whereas in diabetic T1KO were preserved. Moreover, conduction velocity in the sciatic nerve was significantly declined in diabetic WT than in T1KO mice. [A1] Immunohistochemical data revealed loss of calcitonin gene-related peptide-positive neurons in the dorsal root ganglia (DRG) in diabetic WT mice, whereas those neurons were protected in diabetic T1KO mice. Moreover, DRG gene expression analysis showed that caspase-3 and -9 were reduced in T1KO compared with WT mice. To investigate the mechanisms underlying these events, the role of pericytes, which are important CS-expressing components of the blood-nerve barrier (BNB), was explored. Assessment of the retinal vessels, which represent a good model to evaluate pericyte functions and easier to access than peripheral nerves, revealed a higher proportion of pericyte-deficient retina blood vessels in diabetic WT than in T1KO mice upon administration of a platelet-derived growth factor receptor (PDGFR) β antibody. Therefore, pericytes in T1KO mice were more resistant against PDGFRβ antibody than those of WT mice. Thus, it is possible that the BNB in T1KO mice may be more resistant against external stress, including diabetes. Conclusions: Reduced CS production may induce beneficial effects to prevent diabetic neuropathy by improving pericyte function. Disclosure H. Ishiguro: None. T. Ushiki: None. A. Honda: None. T. Mikami: None. H. Kitagawa: None. K. Sango: None. M. Masuko: None. M. Igarashi: None. H. Sone: Research Support; Self; Astellas Pharma Inc., Eisai Co., Ltd., Kyowa Kirin Co., Ltd., Novo Nordisk, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Co.
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- 2021
24. Osteoclastogenic Potential of Tissue-Engineered Periosteal Sheet: Effects of Culture Media on the Ability to Recruit Osteoclast Precursors
- Author
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Mari Watanabe, Yoko Fujimoto, Suguru Tamura, Hajime Ishiguro, Kohya Uematsu, Masaki Nagata, Tomoyuki Kawase, Riuko Ohashi, Takashi Ushiki, and Yoichi Ajioka
- Subjects
0301 basic medicine ,Male ,Periodontium ,green fluorescent protein ,Osteoclasts ,Biocompatible Materials ,Matrix (biology) ,lcsh:Chemistry ,0302 clinical medicine ,Osteogenesis ,Materials Testing ,lcsh:QH301-705.5 ,Spectroscopy ,Tartrate-resistant acid phosphatase ,Mice, Inbred BALB C ,Chemistry ,General Medicine ,Computer Science Applications ,Cell biology ,Haematopoiesis ,medicine.anatomical_structure ,periosteal sheets ,Female ,Stem cell ,Adult ,bone marrow transplantation ,Green Fluorescent Proteins ,Mice, Transgenic ,Article ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,Young Adult ,Osteoclast ,medicine ,Animals ,Humans ,Physical and Theoretical Chemistry ,Molecular Biology ,osteoclastogenesis ,Tissue Engineering ,CD11b ,Organic Chemistry ,030206 dentistry ,In vitro ,Culture Media ,Transplantation ,Mice, Inbred C57BL ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,Bone marrow ,tartrate-resistant acid phosphatase - Abstract
Cell culture media influence the characteristics of human osteogenic periosteal sheets. We have previously found that a stem cell medium facilitates growth and collagen matrix formation in vitro and osteogenesis in vivo. However, it has not yet been demonstrated which culture medium is superior for osteoclastogenesis, a prerequisite for reconstruction of normal bone metabolic basis. To address this question, we compared chemotaxis and osteoclastogenesis in tissue-engineered periosteal sheets (TPSs) prepared with two types of culture media. Periosteal tissues obtained from adult volunteers were expanded with the conventional Medium 199 or with the stem cell medium, MesenPRO. Hematopoietic enhanced-green-fluorescent-protein (EGFP)-nude mice were prepared by γ-irradiation of Balb/c nu/nu mice and subsequent transplantation of bone marrow cells from CAG-EGFP C57BL/6 mice. TPSs were implanted subcutaneously into the chimeric mice and retrieved after intervals for immunohistopathological examination. EGFP+ cells were similarly recruited to the implantation site in both the TPSs prepared, whereas the distribution of CD11b+ cells was significantly lower in the TPS prepared with the stem cell medium. Instead, osteoclastogenesis was higher in the TPS prepared with the stem cell medium than in the one prepared with the conventional medium. These findings suggest that the stem cell medium is preferable for the preparation of more functional TPSs.
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- 2021
25. Weight and cardiometabolic risk among adolescents in Agano city, Japan: NICE EVIDENCE Study-Agano 1
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Sakiko Yoshizawa, Morikawa, Kazuya, Fujihara, Yasunaga, Takeda, Mariko, Hatta, Chika, Horikawa, Masahiro, Ishizawa, Masahiko, Yamamoto, Tomonobu, Shiraishi, Hajime, Ishiguro, Takaho, Yamada, Yohei, Ogawa, and Hirohito, Sone
- Subjects
Male ,Pediatric Obesity ,Cross-Sectional Studies ,Adolescent ,Japan ,Cardiovascular Diseases ,Risk Factors ,Humans ,Female ,Overweight ,Child ,Body Mass Index - Abstract
Pediatric obesity is associated with clustered cardiometabolic risk and the future incidence of cardiovascular disease. However, few studies have determined the effect of pediatric obesity in Asia, where obesity is less common than in Western countries. We aimed to clarify whether weight status including underweight and slightly overweight is associated with metabolic risk factors in Japanese adolescents.We performed a cross-sectional analysis of 2241 adolescents aged 13-14 years. Participants were classified as underweight, normal weight, slightly overweight, overweight, or obese according to the International Obesity Task Force. The clustered cardiometabolic risk (Z-CMR) was estimated by summing standardized sex-specific Z scores of mean arterial pressure (MAP), non-high-density lipoprotein cholesterol (non-HDLC), and HbA1c.Linear regression analysis showed that MAP, non-HDL-C, and Z-CMR were higher in the slightly overweight, overweight, and obese groups than in the normal weight group after adjusting for confounders. Compared with the normal weight group, the slightly overweight, overweight, and obese groups had higher prevalence of high BP [odds ratios (ORs): 1.38 (95% CI, 1.03, 1.85); 2.63 (1.77, 3.91); and 2.39 (1.57, 3.64), respectively]. Compared with the normal weight group, underweight boys, but not girls, had a lower prevalence of high Z-CMR [OR=0.20 (0.05, 0.84)].Adolescents classified as slightly overweight had higher levels of BP, serum lipids, and clustered cardiometabolic risk than those classified as normal weight. This observation showed significant associations between weight status and cardiometabolic risk factors during adolescence even in East Asians.
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- 2020
26. Distinct effects of chondroitin sulfate on hematopoietic cells and the stromal microenvironment in bone marrow hematopoiesis
- Author
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Hajime Ishiguro, Tomoyuki Tanaka, Yaeko Nakajima-Takagi, Tadahisa Mikami, Takashi Ushiki, Asami Kawasaki, Masayoshi Masuko, Motohiko Oshima, Takayuki Katagiri, Hiroshi Kitagawa, Michihiro Igarashi, Atsushi Iwama, Hirohito Sone, and Shun Uemura
- Subjects
Cancer Research ,Stromal cell ,Bone Marrow Cells ,Extracellular matrix ,Glycosaminoglycan ,chemistry.chemical_compound ,Genetics ,medicine ,Animals ,Chondroitin sulfate ,Progenitor cell ,Stem Cell Niche ,Molecular Biology ,Bone Marrow Transplantation ,Mesenchymal stem cell ,Chondroitin Sulfates ,Cell Biology ,Hematology ,Hematopoietic Stem Cells ,Cell biology ,Hematopoiesis ,Mice, Inbred C57BL ,Haematopoiesis ,medicine.anatomical_structure ,chemistry ,Bone marrow - Abstract
The bone marrow (BM) microenvironment, known as the BM niche, regulates hematopoiesis but is also affected by interactions with hematopoietic cells. Recent evidence indicates that extracellular matrix components are involved in these interactions. Chondroitin sulfate (CS), a glycosaminoglycan, is a major component of the extracellular matrix; however, it is not known whether CS has a physiological role in hematopoiesis. Here, we analyzed the functions of CS in hematopoietic and niche cells. CSGalNAcT1, which encodes CS N-acetylgalactosaminyltransferase-1 (T1), a key enzyme in CS biosynthesis, was highly expressed in hematopoietic stem and progenitor cells (HSPCs) and endothelial cells (ECs), but not in mesenchymal stromal cells (MSCs) in BM. In T1 knockout (T1KO) mice, a greater number of HSPCs existed compared with the wild-type (WT), but HSPCs from T1KO mice showed significantly impaired repopulation in WT recipient mice on serial transplantation. RNA sequence analysis revealed the activation of IFN-α/β signaling and endoplasmic reticulum stress in T1KO HSPCs. In contrast, the number of WT HSPCs repopulated in T1KO recipient mice was larger than that in WT recipient mice after serial transplantation, indicating that the T1KO niche supports repopulation of HSPCs better than the WT niche. There was no obvious difference in the distribution of vasculature and MSCs between WT and T1KO BM, suggesting that CS loss alters vascular niche functions without affecting its structure. Our results revealed distinct roles of CS in hematopoietic cells and BM niche, indicating that crosstalk between these components is important to maintain homeostasis in BM.
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- 2020
27. 1267-P: Physical Fitness (PF), Weight Status, and Metabolic Risk in Japanese Adolescents
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Hajime Ishiguro, Mizuki Takeuchi, Sakiko Y. Morikawa, Hirohito Sone, Izumi Ikeda, Yasunaga Takeda, Mariko Hatta, Rina Nedachi, Kazuya Fujihara, and Takaho Yamada
- Subjects
medicine.medical_specialty ,business.industry ,Endocrinology, Diabetes and Metabolism ,Physical fitness ,Metabolic risk ,Cardiorespiratory fitness ,Overweight ,Standard score ,medicine.disease ,Obesity ,Internal medicine ,Linear regression ,Internal Medicine ,medicine ,medicine.symptom ,business ,Weight status - Abstract
Although cardiorespiratory fitness (CRF) and muscular fitness (MF) are modifiable factors for metabolic risk, the role of weight status in these factors has not been clarified in the pediatric population. We examined associations between metabolic indices and CRF or MF according to weight categories and compared the prevalence of metabolic abnormalities among four groups stratified by combinations of PF and weight categories. Cross-sectionally analyzed were 1744 Japanese adolescents aged 13-14 years. The PF test included measurements of CRF (20-m shuttle run test), upper-limb strength (handgrip test), lower limb strength (standing long jump test), and muscular endurance (sit-ups). Participants were classified as non-overweight (non-OW) or overweight/obese (OW) according to BMI cutoffs by the International Obesity Task Force. Metabolic risk was defined as ≥1 SD of the clustered metabolic risk (estimated by summing standardized sex-specific Z scores of HbA1c, MAP, and non-HDL-C). After adjusting for BMI and other characteristics, linear regression analysis showed that non-HDL-C was inversely associated with CRF in the non-OW group (P In summary, relationships of metabolic risk with components of PF differed according to weight status. In OW adolescents, MF might be more useful for stratifying metabolic risk than CRF. Disclosure S.Y. Morikawa: None. K. Fujihara: None. R. Nedachi: None. I. Ikeda: None. Y. Takeda: None. M. Takeuchi: None. M. Hatta: None. H. Ishiguro: None. T. Yamada: None. H. Sone: Research Support; Self; Kyowa Hakko Kirin Co., Ltd., Novartis AG, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Co.
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- 2020
28. 841-P: Predictive Ability of Incident Type 2 Diabetes Mellitus (T2DM) Using Machine Learning Algorithms: A Meta-analysis
- Author
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Masahiko Yamamoto, Hirohito Sone, Hajime Ishiguro, Kiminori Kato, Midori Iwanaga, Satoru Kodama, Takaaki Sato, Kazuya Fujihara, and Takaho Yamada
- Subjects
Diabetes risk ,medicine.diagnostic_test ,Receiver operating characteristic ,business.industry ,Endocrinology, Diabetes and Metabolism ,Type 2 Diabetes Mellitus ,Machine learning ,computer.software_genre ,Likelihood ratios in diagnostic testing ,Confidence interval ,Support vector machine ,Meta-analysis ,Internal Medicine ,medicine ,Blood test ,Artificial intelligence ,business ,computer ,Algorithm - Abstract
Machine learning (ML) methods receive increasing attention for diabetes risk assessment. This meta-analysis quantitatively assessed the predictive ability of ML algorithms for incident T2DM. Electronic literature searches were done using MEDLINE and EMBASE for longitudinal studies whereby an index test was a model constructed by ML algorithms for predicting incident T2DM. Reference standard was a blood test or physicians’ diagnosis confirming T2DM. The model was restricted to algorithms that required the aid of ML (e.g., neural network, support vector machine, etc.). The 2x2 contingency data (i.e., true-positive, false-positive, false-negative, and true-negative) were pooled with a hierarchical summary receiver operating characteristic model. Pooled sensitivity and specificity (95% confidence interval (CI)) of 8 eligible studies were 0.70 (0.66-0.74) and 0.78 (0.69-0.85), respectively. (Figure) Positive likelihood ratio (PLR) calculated as sensitivity/(1-specificity) and negative likelihood ratio (NLR) calculated as (1-sensitivity)/specificity were 3.25 (95%CI, 2.25-4.58) and 0.38 (95% CI, 0.34-0.43), respectively. We conclude that existing prediction models are not yet satisfactory considering that PLR>5 moderately increases the probability of a disease given a positive test and NLR Disclosure S. Kodama: None. T. Sato: None. M. Yamamoto: None. H. Ishiguro: None. M. Iwanaga: None. K. Fujihara: None. T. Yamada: None. K. Kato: None. H. Sone: Research Support; Self; Kyowa Hakko Kirin Co., Ltd., Novartis AG, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Co.
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- 2020
29. 1988-P: Association between Screen Time Including Smartphone Use and Obesity and Its Associated Lifestyles in School Children in Japan
- Author
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Mayuko H. Yamada, Hajime Ishiguro, Yasuhiro Matsubayashi, Izumi Ikeda, Hirohito Sone, Rina Nedachi, Takaho Yamada, Kazuya Fujihara, and Sakiko Y. Morikawa
- Subjects
Gerontology ,Kyowa hakko ,business.industry ,Endocrinology, Diabetes and Metabolism ,medicine.disease ,Obesity ,Screen time ,Insulin resistance ,Lifestyle factors ,Internal Medicine ,Medicine ,business ,Tv viewing ,Association (psychology) - Abstract
An increase in screen time (ST) is reportedly associated with insulin resistance and obesity in children. However, most studies are of TV viewing but not smartphone use. Also, little is known of the relationship between ST and diet. In this study, we clarified the relationships among ST including smartphone use, lifestyle factors including dietary content, and obesity. Cross-sectionally analyzed were 1393 children between 11-15 years of age. ST (min/day) was classified into 4 groups: In conclusion, the increase in ST including smartphone usage was suggested to be associated with various unfavorable lifestyles including dietary habits that could be connected to obesity. Disclosure I. Ikeda: None. K. Fujihara: None. R. Nedachi: None. S.Y. Morikawa: None. H. Ishiguro: None. M.H. Yamada: None. Y. Matsubayashi: None. T. Yamada: None. H. Sone: Research Support; Self; Kyowa Hakko Kirin Co., Ltd., Novartis AG, Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Co.
- Published
- 2020
30. Prevention of postprandial hypotension-related syncope by caffeine in a patient with long-standing diabetes mellitus
- Author
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Hirohito Sone, Hajime Ishiguro, Keiko Sonoda, Masahiro Ito, Tatsuro Suzuki, Kenichi Iijima, Osamu Hanyu, Kazuo Furukawa, and Hiroshi Morikawa
- Subjects
Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,Blood Pressure ,Pallor ,Syncope ,03 medical and health sciences ,Orthostatic vital signs ,0302 clinical medicine ,Endocrinology ,Diabetic Neuropathies ,Internal medicine ,Diabetes mellitus ,Caffeine ,medicine ,Dietary Carbohydrates ,Humans ,Aged ,Meal ,biology ,business.industry ,Syncope (genus) ,Type 2 Diabetes Mellitus ,biology.organism_classification ,medicine.disease ,Postprandial Period ,Diet ,Postprandial ,Blood pressure ,Autonomic Nervous System Diseases ,Diabetes Mellitus, Type 2 ,030220 oncology & carcinogenesis ,Cardiology ,medicine.symptom ,Hypotension ,business - Abstract
A 74-year-old man who had type 2 diabetes mellitus of a duration of 20 years was admitted for syncope after eating a high carbohydrate meal. Although he had had episodes of pallor or syncope after carbohydrate-rich meals, such as with large amounts of white rice, several times within a year and he had been taken to hospitals emergently, the etiology of these episodes had remained unclear despite his undergoing several studies. Studies did show severe orthostatic hypotension during the head-up tilt test and a decrease in the coefficient of variation of the R-R interval (CVR-R) on resting electrocardiogram, suggesting severe autonomic nervous dysfunction. Because of the episodes of syncope after eating a carbohydrate-rich meal, we investigated whether he had postprandial hypotension (PPH). The 75 g oral glucose tolerance test revealed a significant decrease in his postprandial blood pressure by about 40 mmHg, leading to the diagnosis of PPH. The carbohydrate-rich meal test induced syncope with systolic blood pressure under 40 mmHg. Then 150 mg caffeine was administered before a second carbohydrate-rich meal. The marked decline in postprandial blood pressure was suppressed and plasma noradrenaline levels were gradually increased over a period of 60 minutes. Caffeine could be useful for prevention of postprandial hypotension-related syncope.
- Published
- 2020
31. A case of normotensive incidentally discovered adrenal pheochromocytoma
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Hajime Ishiguro, Hiroyuki Usuda, Ryo Maruyama, Kenzo Kaneko, Fumio Ishizaki, Kyuzi Kamoi, Yuta Yaguchi, Masanori Kaneko, Hironobu Sasano, Kazuya Suzuki, Yuto Yamazaki, Takeshi Komeyama, and Nobumasa Ohara
- Subjects
Pathology ,medicine.medical_specialty ,endocrine system ,endocrine system diseases ,doxazosin ,030209 endocrinology & metabolism ,Case Report ,Case Reports ,Neuroendocrine tumors ,adrenal incidentaloma ,Asymptomatic ,Pheochromocytoma ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Doxazosin ,Adrenal incidentaloma ,neoplasms ,normotensive ,business.industry ,General Medicine ,medicine.disease ,pheochromocytoma ,medicine.anatomical_structure ,nervous system ,030220 oncology & carcinogenesis ,immunohistochemistry ,metaiodobenzylguanidine scintigraphy ,Immunohistochemistry ,Presentation (obstetrics) ,medicine.symptom ,Adrenal medulla ,business ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Key Clinical Message Pheochromocytomas are catecholamine‐producing neuroendocrine tumors that arise from the adrenal medulla. The clinical presentation includes headache, palpitation, and hypertension, but pheochromocytomas are sometimes clinically silent. The present case highlights the importance of biochemical testing for pheochromocytoma in patients with adrenal incidentaloma, even if they are completely normotensive and asymptomatic.
- Published
- 2018
32. Network meta-analysis of the relative efficacy of bariatric surgeries for diabetes remission
- Author
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Masanori Kaneko, Hitoshi Shimano, Hajime Ishiguro, Shiro Tanaka, Satoru Kodama, Kazuo Furukawa, Kazuya Fujihara, Satoshi Matsunaga, Chika Horikawa, Kiminori Kato, Hirohito Sone, Yasuhiro Matsubayashi, and Mayuko Harada
- Subjects
medicine.medical_specialty ,Relative efficacy ,business.industry ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Public Health, Environmental and Occupational Health ,Type 2 Diabetes Mellitus ,030209 endocrinology & metabolism ,medicine.disease ,Curvatures of the stomach ,Duodenal switch ,law.invention ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Meta-analysis ,Diabetes mellitus ,medicine ,030212 general & internal medicine ,business ,Biliopancreatic Diversion - Abstract
Background Bariatric surgery leads to a higher remission rate for type 2 diabetes mellitus than non-surgical treatment. However, it remains unsolved which surgical procedure is the most efficacious. This network meta-analysis aimed to rank surgical procedures in terms of diabetes remission. Methods and findings We electronically searched for randomized controlled trials in which at least one surgical treatment was included among multiple arms and the diabetes remission rate was included in study outcomes. A random-effects network meta-analysis was performed within a frequentist framework. The hierarchy of treatments was expressed as the surface under the cumulative ranking curve value. Results of the analysis of 25 eligible randomized controlled trials that covered non-surgical treatments and eight surgical procedures (biliopancreatic diversion [BPD], BPD with duodenal switch, Roux-en Y gastric bypass, mini gastric bypass [mini-GBP], laparoscopic adjustable gastric banding, laparoscopic sleeve gastrectomy, greater curvature plication and duodenal-jejunal bypass) showed that BPD and mini-GBP had the highest surface under the cumulative ranking curve values among the eight surgical treatments. Conclusion Current network meta-analysis indicated that BPD or mini-GBP achieved higher diabetes remission rates than the other procedures. However, the result needs to be interpreted with caution considering that these procedures were in the minority of bariatric surgeries.
- Published
- 2018
33. Role of fatty liver in the association between obesity and reduced hepatic insulin clearance
- Author
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Hajime Ishiguro, Hirohito Sone, Shiro Tanaka, Masahiko Yamamoto, Yasuhiro Matsubayashi, Hideki Suganami, Satoru Kodama, Akihiro Yoshida, Kazuya Fujihara, and Kohei Kaku
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,030209 endocrinology & metabolism ,Type 2 diabetes ,030204 cardiovascular system & hematology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Insulin resistance ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Obesity ,Aged ,business.industry ,Insulin ,Quantitative insulin sensitivity check index ,Fatty liver ,General Medicine ,Middle Aged ,medicine.disease ,Fatty Liver ,chemistry ,Female ,Insulin Resistance ,Tofogliflozin ,business ,Body mass index - Abstract
AIM Hepatic insulin clearance (HIC) is important in regulating plasma insulin levels. Diminished HIC causes inappropriate hyperinsulinaemia, and both obesity and fatty liver (FL), which are known to decrease HIC, can be found either together in the same patient or on their own. The mechanism by which obesity reduces HIC is presumed to be mediated by FL. However, few reports have examined the role of FL in the relationship between obesity and HIC in type 2 diabetes (T2D) patients. Therefore, our study investigated the association of HIC with clinical factors, including insulin sensitivity indices, focusing on the presence or absence of FL and obesity in T2D patients. METHOD Baseline data from 419 patients with T2D (279 men, 140 women; mean age: 57.6 years; body mass index: 25.5kg/m2) controlled by diet and exercise were analyzed. HIC was calculated from the ratio of fasting c-peptide to fasting insulin levels (HICCIR). Correlation analyses between HICCIR and clinical variables were performed using Pearson's product-moment correlation coefficients and single regression analysis in all participants and in those with obesity and FL either alone or in combination. RESULTS HICCIR was significantly correlated with whole-body insulin sensitivity indices and influenced by FL, but only in the FL group was obesity independently influenced HIC level. HICCIR decreased in those with both FL and obesity compared with those with only one such complication. CONCLUSION HICCIR may be used to evaluate whole-body insulin sensitivity in T2D. Also, compared with obesity, the influence of FL strongly contributed to a reduced HIC. TRIAL REGISTRATION NUMBER These trials were registered by the Japan Pharmaceutical Information Centre clinical trials information (JapicCTI) as 101349 and 101351.
- Published
- 2018
34. Quantitative Relationship Between Cumulative Risk Alleles Based on Genome-Wide Association Studies and Type 2 Diabetes Mellitus: A Systematic Review and Meta-analysis
- Author
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Shiro Tanaka, Hajime Ishiguro, Satoru Kodama, Chika Horikawa, Kazuya Fujihara, Nobumasa Ohara, Yoko Yachi, Kiminori Kato, Hitoshi Shimano, Hirohito Sone, and Osamu Hanyu
- Subjects
Oncology ,medicine.medical_specialty ,endocrine system diseases ,Epidemiology ,type 2 diabetes mellitus ,030209 endocrinology & metabolism ,Genome-wide association study ,Single-nucleotide polymorphism ,Review Article ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Genetic Predisposition to Disease ,Clinical Epidemiology ,030212 general & internal medicine ,Alleles ,Genetic association ,risk allele ,lcsh:R5-920 ,business.industry ,Type 2 Diabetes Mellitus ,nutritional and metabolic diseases ,General Medicine ,Odds ratio ,Confidence interval ,meta-analysis ,Diabetes Mellitus, Type 2 ,Meta-analysis ,genome-wide association studies ,business ,lcsh:Medicine (General) ,Genome-Wide Association Study - Abstract
Many epidemiological studies have assessed the genetic risk of having undiagnosed or of developing type 2 diabetes mellitus (T2DM) using several single nucleotide polymorphisms (SNPs) based on findings of genome-wide association studies (GWAS). However, the quantitative association of cumulative risk alleles (RAs) of such SNPs with T2DM risk has been unclear. The aim of this meta-analysis is to review the strength of the association between cumulative RAs and T2DM risk. Systematic literature searches were conducted for cross-sectional or longitudinal studies that examined odds ratios (ORs) for T2DM in relation to genetic profiles. Logarithm of the estimated OR (log OR) of T2DM for 1 increment in RAs carried (1-ΔRA) in each study was pooled using a random-effects model. There were 46 eligible studies that included 74,880 cases among 249,365 participants. In 32 studies with a cross-sectional design, the pooled OR for T2DM morbidity for 1-ΔRA was 1.16 (95% confidence interval [CI], 1.13–1.19). In 15 studies that had a longitudinal design, the OR for incident T2DM was 1.10 (95% CI, 1.08–1.13). There was large heterogeneity in the magnitude of log OR (P < 0.001 for both cross-sectional studies and longitudinal studies). The top 10 commonly used genes significantly explained the variance in the log OR (P = 0.04 for cross-sectional studies; P = 0.006 for longitudinal studies). The current meta-analysis indicated that carrying 1-ΔRA in T2DM-associated SNPs was associated with a modest risk of prevalent or incident T2DM, although the heterogeneity in the used genes among studies requires us to interpret the results with caution.
- Published
- 2018
35. Unstable bodyweight and incident type 2 diabetes mellitus: A meta-analysis
- Author
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Hitoshi Shimano, Kiminori Kato, Satoru Kodama, Hirohito Sone, Kazuya Fujihara, Chika Horikawa, Nobumasa Ohara, Hajime Ishiguro, Shiro Tanaka, Osamu Hanyu, and Yoko Yachi
- Subjects
medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,MEDLINE ,030209 endocrinology & metabolism ,Weight Gain ,Weight cycling ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Internal medicine ,Weight Loss ,Type 2 diabetes mellitus ,Internal Medicine ,medicine ,Humans ,030212 general & internal medicine ,business.industry ,Weight change ,Type 2 Diabetes Mellitus ,Articles ,General Medicine ,Publication bias ,medicine.disease ,Confidence interval ,Clinical Science and Care ,Diabetes Mellitus, Type 2 ,Meta-analysis ,Relative risk ,Meta‐analysis ,Original Article ,business - Abstract
Aims/Introduction The present meta-analysis aimed to clarify the association of unstable bodyweight with the risk of type 2 diabetes mellitus, an association that has been controversial among longitudinal studies., Materials and Methods An electronic literature search using EMBASE and MEDLINE was followed up to 31 August 2016. The relative risks (RRs) of type 2 diabetes mellitus in individuals with unstable bodyweight were pooled using the inverse variance method., Results Eight studies were eligible for the meta-analysis. The median duration of measurements of weight change and follow-up years for ascertaining type 2 diabetes mellitus were 13.5 and 9.4 years, respectively. The pooled RR for the least vs most stable category was 1.33 (95% confidence interval 1.12–1.57). Between-study heterogeneity was statistically significant (P = 0.048). Whether type 2 diabetes mellitus was ascertained by blood testing explained 66.0% of the variance in the logarithm of RR (P = 0.02). In three studies in which blood testing was carried out, type 2 diabetes mellitus risk was not significant (RR 1.06, 95% confidence interval 0.91–1.25). Furthermore, publication bias that inflated type 2 diabetes mellitus risk was statistically detected by Egger's test (P = 0.09)., Conclusions Unstable bodyweight might be modestly associated with the elevated risk of type 2 diabetes mellitus; although serious biases, such as diagnostic suspicion bias and publication bias, made it difficult to assess this association.
- Published
- 2017
36. 1335-P: Weight Status and Cardiometabolic Risk Factors among Adolescents in Japan
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Sakiko Y. Morikawa, Dai Ishii, Masahiro Ishizawa, Hajime Ishiguro, Hirohito Sone, Yasuhiro Matsubayashi, Takaho Yamada, Satoru Kodama, Rina Nedachi, and Kazuya Fujihara
- Subjects
medicine.medical_specialty ,business.industry ,Endocrinology, Diabetes and Metabolism ,Incidence (epidemiology) ,Blood lipids ,Odds ratio ,Overweight ,Standard score ,medicine.disease ,Obesity ,Diabetes mellitus ,Internal medicine ,Internal Medicine ,medicine ,Underweight ,medicine.symptom ,business - Abstract
Pediatric obesity is associated with clustered cardiometabolic risk and the future incidence of diabetes. However, few studies have determined the effect of pediatric obesity in Asia, where obesity is less common than in Western countries. Thus, we clarified whether metabolic risk factors are associated with weight status including slight overweight and underweight among Japanese adolescents. We performed a cross-sectional analysis of 2241 Japanese adolescents (1180 boys) aged 13-14 years whose height, weight, HbA1c, blood pressure (BP), and serum lipids were measured. Participants were classified as underweight, normal weight, slightly overweight, overweight, or obese according to both the universal and Asian cutoffs of the International Obesity Task Force, which correspond to adult BMI cutoffs. The clustered cardiometabolic risk (Z-CMR) was estimated by summing standardized sex-specific Z scores of HbA1c, mean arterial pressure (MAP), and non-high-density lipoprotein cholesterol (non-HDL-C). Linear regression analysis showed that MAP (sex-specific Z score), non-HDL-C (sex-specific Z score), and Z-CMR were higher in the slightly overweight, overweight, and obese groups than in the normal weight group after adjusting for confounders. Compared with the normal weight group, the slightly overweight, overweight, and obese groups had significantly higher odds ratios (ORs) for high BP [OR = 1.38 (95% CI, 1.03-1.85); OR = 2.63 (1.77-3.91); and OR = 2.39 (1.57-3.64), respectively]. Compared with the normal weight group, underweight boys, but not girls, had a significantly lower OR for high Z-CMR [OR = 0.20 (0.05-0.84)]. Our results indicated that adolescents classified as slightly overweight had higher levels of BP, serum lipids (but not HbA1c), and clustered cardiometabolic risk than those classified as normal weight. This observation demonstrated that significant associations between weight status and cardiometabolic risk factors could be seen during adolescence even in East Asia. Disclosure S.Y. Morikawa: None. K. Fujihara: None. D. Ishii: None. R. Nedachi: None. M. Ishizawa: None. H. Ishiguro: None. Y. Matsubayashi: None. T. Yamada: None. S. Kodama: None. H. Sone: Research Support; Self; Astellas Pharma Inc., Boehringer Ingelheim Pharmaceuticals, Inc., Daiichi Sankyo Company, Limited, Kowa Pharmaceutical Europe Co. Ltd., Kyowa Hakko Kirin Co., Ltd., Novo Nordisk Inc., Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Company Limited, Teijin Pharma Limited.
- Published
- 2019
37. 1910-P: SOCS3 Deficiency on a High-Fat Diet Accelerates Systemic Inflammation and Results in Lethal Myeloid Hematopoiesis without Obesity and Adiposity
- Author
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Hitoshi Shimano, Takayuki Katagiri, Hajime Ishiguro, Takashi Ushiki, Tatsuya Suwabe, Warren S. Alexander, Kaori Cho, Hirohito Sone, Masayoshi Masuko, Yoshimi Nakagawa, and Hideyo Hirai
- Subjects
medicine.medical_specialty ,Myeloid ,business.industry ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,digestive, oral, and skin physiology ,Fatty liver ,medicine.disease ,Systemic inflammation ,Granulopoiesis ,Endocrinology ,Cytokine ,medicine.anatomical_structure ,Insulin resistance ,Internal medicine ,Internal Medicine ,medicine ,SOCS3 ,Bone marrow ,medicine.symptom ,business - Abstract
Introduction: Suppressors of cytokine signaling (SOCS) are negative regulators of cytokine signaling; SOCS3 controls myeloid hematopoiesis by suppressing G-CSF. SOCS3 haploinsufficiency attenuates diet-induced obesity by enhanced leptin sensitivity while improving insulin resistance. We established SOCS3 conditional knock-out (KO) mice to elucidate how high fat diets (HFD) affect inflammation and obesity in complete SOCS3 deficiency. Methods: Embryonic lethality of complete SOCS3 deficiency was overcome using a conditional Socs3 allele inactivated by tamoxifen (TAM)-inducible cre-recombinase with SOCS3fl/flRosa26-CreERT2T/+ mice. They were fed HFD (60 kcal% fat) or control chaw (10 kcal% fat) starting at 4 weeks of age and TAM or vehicle administration was done at 8 weeks. Results: Although insulin resistance improved and obesity and fatty liver were not confirmed in SOCS3-KO with HFD (S3HFD), the S3HFD group looked moribund around day 30 post-TAM by systemic inflammation; their mean survival was 62.5 days after TAM. Other groups did not look moribund. Significant granulopoiesis was also observed in S3HFD (peripheral blood neutrophil counts 29301 ± 20581/mm3). This myeloid hematopoiesis occurred in the spleen and not the bone marrow. S3HFD exhibited the progression of various granulocytes from progenitor cells (c-kitint∼highLy6G-) to mature granulocytes (c-kit-Ly6G+).Hematopoietic infiltration was confirmed in the liver in S3HFD with granulocytes, monocytes, and macrophages. Inflammasome-associated genes (IL-1β and Caspase1) were elevated in the liver at day 65 post-TAM in S3HFD. Conclusion: SOCS3 deficiency showed myeloid proliferation under HFD without obesity and adiposity. SOCS3-KO may help in the clarification of the specific mechanism of myeloid proliferation and invasion in HFD-induced inflammation. Disclosure K. Cho: None. T. Ushiki: None. H. Ishiguro: None. T. Katagiri: None. T. Suwabe: None. H. Hirai: None. Y. Nakagawa: None. M. Masuko: None. W. Alexander: None. H. Shimano: None. H. Sone: Research Support; Self; Astellas Pharma Inc., Boehringer Ingelheim Pharmaceuticals, Inc., Daiichi Sankyo Company, Limited, Kowa Pharmaceutical Europe Co. Ltd., Kyowa Hakko Kirin Co., Ltd., Novo Nordisk Inc., Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Company Limited, Teijin Pharma Limited.
- Published
- 2019
38. 598-P: Lower Hematocrit Is Predictive of Treatment-Required Eye Diseases in Japanese Patients with Diabetes Mellitus
- Author
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Yasuhiro Matsubayashi, Taeko Osawa, Mayuko Harada, Hirohito Sone, Hiroyasu Seida, Kazuya Fujihara, Nauta Yamanaka, Hiroshi Suzuki, Masahiko Yamamoto, Hajime Ishiguro, and Masahiro Ishizawa
- Subjects
medicine.medical_specialty ,Medical treatment ,medicine.diagnostic_test ,Proportional hazards model ,business.industry ,Endocrinology, Diabetes and Metabolism ,Hazard ratio ,Diabetic retinopathy ,Hematocrit ,medicine.disease ,Gastroenterology ,Confidence interval ,Diabetes mellitus ,Internal medicine ,Internal Medicine ,medicine ,business ,Retinopathy - Abstract
Hematological markers such as hematocrit (Hct), hemoglobin (Hb) or red blood cell (RBC) count were reported to be associated with the development of diabetic retinopathy with conflicting results. Moreover, which of these markers is the best to predict incident retinopathy remains unknown. To clarify this, we utilized ICD-10 and medical treatment codes from a nationwide claims database that included 7,182 participants (1,490 women, mean age 50.8 y, HbA1c 6.9%, mean follow-up period 5.5y) classified as having diabetes mellitus or vision-threatening treatment-required diabetic eye diseases (TRDED). Multivariate Cox regression model was used to identify predictors of TRDED. During the study period, 177 patients developed TRDED (4.5/1000 person-years). Cox analysis demonstrated that the lower Hcts were significant predictors TRDED and lower Hcts were more predictive than lower RBC counts. The Hb level was not significantly associated with TRDED. Hazard ratios (HRs) per 1 standard deviation (SD) increase in Hct, RBC count and Hb for incident TRDED were 0.792 (95% confidence interval [CI], 0.666-0.942), 0.874(0.765-0.998), and 0.861 (0.725-1.023) as continuous variables, respectively. The interaction between Hct and RBC count was not statistically significant. Multivariate tertile analysis also revealed that a lower Hct was more predictive of TRDED (HR 2.03 [95% CI, 1.33-3.08] for the bottom [≤43.4%] vs. the top [≥46.7%] tertile [P value = 0.001]) than lower RBC counts (HR 1.54 [95% CI, 0.99-2.38] for the bottom [≤468×104/m3] vs. the top [≥507×104/m3] [P value = 0.054]). These findings demonstrated that lower Hcts were significant predictor of vision-threatening retinopathy for people with diabetes and more useful than the RBC count or Hb. Association of the influence of these three markers on severe retinopathy awaits further investigation. Disclosure M. Yamamoto: None. K. Fujihara: None. T. Osawa: None. M. Harada: None. M. Ishizawa: None. H. Suzuki: None. H. Ishiguro: None. H. Seida: None. N. Yamanaka: None. Y. Matsubayashi: None. H. Sone: Research Support; Self; Astellas Pharma Inc., Boehringer Ingelheim Pharmaceuticals, Inc., Daiichi Sankyo Company, Limited, Kowa Pharmaceutical Europe Co. Ltd., Kyowa Hakko Kirin Co., Ltd., Novo Nordisk Inc., Ono Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Company Limited, Teijin Pharma Limited.
- Published
- 2019
39. Pulse Pressure is a Stronger Predictor Than Systolic Blood Pressure for Severe Eye Diseases in Diabetes Mellitus
- Author
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Hiruma Hasebe, Satoru Kodama, Nauta Yamanaka, Shiro Tanaka, Kazuya Fujihara, Yasuhiro Matsubayashi, Hajime Ishiguro, Taeko Osawa, Hirohito Sone, Hiroyasu Seida, Masanori Kaneko, Masahiro Ishizawa, and Masahiko Yamamoto
- Subjects
medicine.medical_specialty ,systolic blood pressure ,Eye Diseases ,Epidemiology ,Diabetic mellitus ,030209 endocrinology & metabolism ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Diabetes mellitus ,Internal medicine ,medicine ,Humans ,Adverse effect ,hemoglobin A1c ,Original Research ,Diabetic Retinopathy ,business.industry ,vision‐threatening treatment‐required diabetic eye diseases ,blood pressure ,Diabetic retinopathy ,pulse pressure ,medicine.disease ,Pulse pressure ,Blood pressure ,Diabetes Mellitus, Type 1 ,diabetic mellitus ,Cardiology ,Cardiology and Cardiovascular Medicine ,Low diastolic blood pressure ,business ,Diabetic Angiopathies - Abstract
Background Evidence of the role of systolic blood pressure (SBP) in development of severe diabetic retinopathy is not strong, although the adverse effect of low diastolic blood pressure has been a partial explanation. We assessed the predictive ability of incident severe diabetic retinopathy between pulse pressure (PP) which considers both SBP and diastolic blood pressure, compared with SBP. Methods and Results Eligible patients (12 242, 83% men) aged 19 to 72 years from a nationwide claims database were analyzed for a median observational 4.8‐year period. Severe diabetic retinopathy was defined as vision‐threatening treatment‐required diabetic eye diseases. Multivariate Cox regression analysis revealed that hazard ratios (95% CI) of treatment‐required diabetic eye diseases for 1 increment of standard deviation and the top tertile compared with the bottom tertile were 1.39 (1.21–1.60) and 1.72 (1.17–2.51), respectively, for PP and 1.22 (1.05–1.41) and 1.43 (0.97–2.11), respectively, for SBP adjusted for age, sex, body mass index, hemoglobin A1c, fasting plasma glucose, lipids, and smoking status. In a model with SBP and PP simultaneously as covariates, the hazard ratios of only PP (hazard ratios [95% CI], 1.57 [1.26–1.96]) but not SBP (0.85 [0.68–1.07]) were statistically significant. Delong test revealed a significant difference in the area under the receiver operating characteristic curve between PP and SBP (area under the receiver operating characteristic curve [95% CI], 0.58 [0.54–0.63] versus 0.54 [0.50–0.59]; P=0.03). The strongest predictor remained as hemoglobin A1c (area under the receiver operating characteristic curve [95% CI], 0.80 [0.77–0.84]). Conclusions After excluding the significant impact of glycemic control, PP in comparison with SBP is a better predictor of severe diabetic retinopathy, suggesting a role of diastolic blood pressure and arterial stiffness in pathology., See Editorial Dart
- Published
- 2019
40. In Search of the Ideal Resistance Training Program to Improve Glycemic Control and its Indication for Patients with Type 2 Diabetes Mellitus: A Systematic Review and Meta-Analysis
- Author
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Chika Horikawa, Satoru Kodama, Nobumasa Ohara, Osamu Hanyu, Yoko Yachi, Kazuya Fujihara, Hitoshi Shimano, Reiko Hirasawa, Hirohito Sone, Ayumi Sugawara Hirose, and Hajime Ishiguro
- Subjects
Blood Glucose ,medicine.medical_specialty ,Sports medicine ,030209 endocrinology & metabolism ,Physical Therapy, Sports Therapy and Rehabilitation ,Body Mass Index ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Diabetes mellitus ,Linear regression ,medicine ,Humans ,Orthopedics and Sports Medicine ,030212 general & internal medicine ,Glycemic ,Glycated Hemoglobin ,business.industry ,Resistance Training ,medicine.disease ,Confidence interval ,Standard error ,Diabetes Mellitus, Type 2 ,Meta-analysis ,Physical therapy ,business ,Body mass index - Abstract
Resistance training (RT) is effective for glycemic control in type 2 diabetes mellitus (T2DM) patients. However, the characteristics of an RT program that will maximize its effect and those of patients that will especially benefit from RT are unknown. The objectives of this systematic review were to identify via a comprehensive meta-analysis the characteristics of an RT program for patients with T2DM that might increase the patients’ improvement in glycemic control and the characteristics of patients that will benefit from RT. Electronic-based literature searches of MEDLINE and EMBASE entries from 1 January 1966 to 25 August 2014 were conducted to identify clinical trials examining the effect of RT on glycemic control among patients with T2DM. Study keywords were text words and thesaurus terms related to RT and T2DM. Studies were included if they (1) were clinical trials consisting of two groups with and without RT exercise intervention; (2) had an intervention period of at least 5 weeks; (3) clarified that all patients had T2DM; and (4) reported or made it possible to estimate the effect size [i.e., change in glycosylated hemoglobin (HbA1c) in the RT group minus that in the control group] and its corresponding standard error. The effect size in each study was pooled with a random-effects model. Analyses were stratified by several key characteristics of the patients and RT exercise programs; meta-regression analysis was then used to detect a difference in the effect size among strata within each factor. Linear regression analyses were added by entering each of the following profiles: patients’ baseline characteristics [mean baseline age, body mass index (BMI), and HbA1c levels] and exercise characteristics (total sets per week, total sets per bout of exercise, frequency, and intensity). There were 23 eligible studies comprising 954 patients with T2DM. The pooled effect size (95 % confidence interval) was −0.34 % (−0.53 to −0.16). A program with multiple sets (≥21 vs.
- Published
- 2015
41. Test Strip–Positive Proteinuria and Its Combination with Low eGFR Are Predictive of Treatment-Required Eye Diseases in Japanese Patients with Diabetes Mellitus
- Author
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Nauta Yamanaka, Masahiro Ishizawa, Hajime Ishiguro, Mayuko Harada, Hirohito Sone, Masahiko Yamamoto, Taeko Osawa, Yasuhiro Matsubayashi, Hiroyasu Seida, Hiroshi Suzuki, and Kazuya Fujihara
- Subjects
medicine.medical_specialty ,Proteinuria ,Medical treatment ,business.industry ,Proportional hazards model ,Endocrinology, Diabetes and Metabolism ,Hazard ratio ,Urine ,medicine.disease ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,Medicine ,In patient ,medicine.symptom ,business ,Retinopathy - Abstract
Proteinuria or low eGFR was separately shown to be associated with the development of retinopathy in patients with diabetes. However, it remains unclear whether semi-quantitatively assessed proteinuria by test strips either combined or not combined with low eGFR is predictive of incident retinopathy, especially in its advanced stages.To clarify this, we utilized ICD-10 and medical treatment codes from a nationwide claims database that included 6,100 participants (1,074 women, mean age 49.3 y, HbA1c 6.9%) classified as having diabetes mellitus or treatment-required diabetic eye diseases (TRDED) during an 8-y period. Urinary protein was assessed by test strip and a result of ≥1+ indicated proteinuria, which reportedly corresponds to a urine protein concentration of ≥30 mg/dL. Multivariate Cox regression model was used to identify predictors of TRDED. During the study period, 147 patients developed TRDED (4.5/1000 person-years). Cox analysis showed that proteinuria was a significant and independent predictor of TRDED but not the serum creatinine level. Hazard ratios (HRs) for proteinuria, low eGFR (defined as Disclosure M. Yamamoto: None. K. Fujihara: None. T. Osawa: None. M. Harada: None. M. Ishizawa: None. H. Ishiguro: Research Support; Self; MSD K.K., Sanofi K.K., Eli Lilly and Company. H. Suzuki: None. H. Seida: None. N. Yamanaka: None. Y. Matsubayashi: None. H. Sone: Research Support; Self; Novo Nordisk Inc., Eli Lilly and Company, MSD K.K., Chugai Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Development Center Asia, Pte. Ltd., Daiichi Sankyo Company, Limited, Ono Pharmaceutical Co., Ltd., Kyowa Hakko Kirin Co., Ltd., Sanofi, Kowa Pharmaceuticals America, Inc., Eisai Inc..
- Published
- 2018
42. Education for Family Members Is Effective for Improved Glycemic Control of Patients with Type 2 Rather than Type 1 Diabetes Mellitus—A Meta-analysis
- Author
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Shiro Tanaka, Satoru Kodama, Hajime Ishiguro, Kiminori Kato, Kazuya Fujihara, Hirohito Sone, Yoko Yachi, Masahiro Ishizawa, Chika Horikawa, and Satoshi Matsunaga
- Subjects
medicine.medical_specialty ,Type 1 diabetes ,Kyowa hakko ,Family education ,business.industry ,Endocrinology, Diabetes and Metabolism ,medicine.disease ,Clinical trial ,Diabetes mellitus ,Family medicine ,Meta-analysis ,Internal Medicine ,Medicine ,business ,Glycemic - Abstract
Although the importance of support of patients by family members is emphasized for diabetes care, evidence of achieving adequate glycemic control (GC) through instructing family members to help in self-management care of diabetes patients has not been established. This meta-analysis assessed the effect of family-oriented diabetes programs on GC. The pooled A1C change shown in 31 studies that were retrieved from systematic literature searches for clinical trials presenting data on the net effect of family education on the reduction in glycohemoglobin A1C (A1C) (i.e., difference in A1C change between intervention groups involving family members and that not involving family members [control group]) was -0.45% (PP Disclosure S. Kodama: None. K. Fujihara: None. H. Ishiguro: Research Support; Self; MSD K.K., Sanofi K.K., Eli Lilly and Company. C. Horikawa: None. Y. Yachi: None. M. Ishizawa: None. S. Matsunaga: None. S. Tanaka: None. K. Kato: None. H. Sone: Research Support; Self; Novo Nordisk Inc., Eli Lilly and Company, MSD K.K., Chugai Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Development Center Asia, Pte. Ltd., Daiichi Sankyo Company, Limited, Ono Pharmaceutical Co., Ltd., Kyowa Hakko Kirin Co., Ltd., Sanofi, Kowa Pharmaceuticals America, Inc., Eisai Inc..
- Published
- 2018
43. Genetically Reduced Chondroitin Sulfate Prevents the Progression of Diabetic Neuropathy
- Author
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Hajime Ishiguro, Michihiro Igarashi, Kazunori Sango, Kaori Cho, Hirohito Sone, Takashi Ushiki, Asami Kawasaki, and Masayoshi Masuko
- Subjects
medicine.medical_specialty ,Diabetic neuropathy ,business.industry ,Endocrinology, Diabetes and Metabolism ,Wild type ,medicine.disease ,Streptozotocin ,Sensory neuron ,Pathophysiology ,chemistry.chemical_compound ,Nociception ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Chondroitin sulfate ,business ,medicine.drug - Abstract
Background: The extracellular matrix is associated with the pathophysiology of diabetic complications; however, the role of chondroitin sulfate (CS) remains unclear. To clarify the effects of CS on diabetic neuropathy (DN), we assessed the effect of genetically reducing CS in mice through disruption of a gene encoding the rate-limiting CS-synthesizing enzyme, i.e., CS N-acetylgalactosaminyltransferase-1 (T1). Methods: T1 knockout (T1KO) mice were generated from the C57BL/6N strain and C57BL/6N were prepared as wild type (WT). Diabetes was induced through streptozotocin injection in 6-week-old male mice. All data were obtained 3 weeks after streptozotocin injection. Results: In the heat radiant test, while thermal nociception in nondiabetic WT and T1KO mice were normal and significantly disrupted in diabetic WT mice, that in diabetic T1KO mice was preserved. The number of plantar peripheral nerve fibers was also significantly decreased in diabetic WT mice; however, that in diabetic T1KO mice were relatively normal. Furthermore, immunohistochemistry revealed loss of calcitonin gene-related peptide-positive neurons in the dorsal root ganglia (DRG) in diabetic WT mice, which contains a cluster of sensory neuron bodies. In contrast, those neurons were protected in diabetic T1KO mice. Hence, nociception and thermoception are preserved in diabetic T1KO mice. To investigate the mechanisms underlying these events, we analyzed gene expression in DRG through real-time polymerase chain reaction and confirmed the suppression of caspase-3 and caspase-9 in diabetic T1KO mice, compared to those in diabetic WT mice. However, levels of Bcl2, TNF-α, MMP9, and reactive oxygen species-related enzymes (HO-1, NOX) did not differ significantly between WT and T1KO diabetic mice. Conclusions: Reduced CS production is suggested to have potentially beneficial effects on preventing DN by suppressing apoptotic signaling and could be a cutting-edge target of clinical application. Disclosure H. Ishiguro: Research Support; Self; MSD K.K., Sanofi K.K., Eli Lilly and Company. T. Ushiki: None. A. Kawasaki: None. K. Cho: None. M. Masuko: None. K. Sango: None. M. Igarashi: None. H. Sone: Research Support; Self; Novo Nordisk Inc., Eli Lilly and Company, MSD K.K., Chugai Pharmaceutical Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Development Center Asia, Pte. Ltd., Daiichi Sankyo Company, Limited, Ono Pharmaceutical Co., Ltd., Kyowa Hakko Kirin Co., Ltd., Sanofi, Kowa Pharmaceuticals America, Inc., Eisai Inc..
- Published
- 2018
44. Meta-analytic research on the relationship between cumulative risk alleles and risk of type 2 diabetes mellitus
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Satoru Kodama, Hitoshi Shimano, Shiro Tanaka, Kazuya Fujihara, Hirohito Sone, Kiminori Kato, Yoko Yachi, Hajime Ishiguro, Osamu Hanyu, Chika Horikawa, and Nobumasa Ohara
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0301 basic medicine ,endocrine system diseases ,business.industry ,Endocrinology, Diabetes and Metabolism ,Confounding ,nutritional and metabolic diseases ,Type 2 Diabetes Mellitus ,030209 endocrinology & metabolism ,Odds ratio ,medicine.disease ,Regression ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Endocrinology ,Diabetes mellitus ,Covariate ,Internal Medicine ,Medicine ,Observational study ,business ,Body mass index ,Demography - Abstract
Background Our aim is to examine the dose–response association between cumulative genetic risk and actual risk of type 2 diabetes mellitus (T2DM) and the influence of adjustment for covariates on T2DM risk through a comprehensive meta-analysis of observational studies. Methods Electronic literature search using EMBASE and MEDLINE (from 2003 to 2014) was conducted for cross-sectional or longitudinal studies that presented the odds ratio (OR) for T2DM in each group with categories based on the total number of risk alleles (RAs) carried (RAtotal) using at least two single-nucleotide polymorphisms. Spline regression model was used to determine the shape of the relationship between the difference from the referent group of each study in RAtotal (ΔRAtotal) and the natural logarithms of ORs (log OR) for T2DM. Results Sixty-five eligible studies that included 68 267 cases among 182 603 participants were analysed. In both crude and adjusted ORs, defined by adjusting the risk for at least two confounders among age, gender and body mass index, the slope of the log OR for T2DM became less steep as the ΔRAtotal increased. In the analysis limited to 14 cross-sectional and four longitudinal studies presenting both crude and adjusted ORs, regression curves of both ORs in relation to ΔRAtotal were almost identical. Conclusion Using only single-nucleotide polymorphisms for T2DM screening was of limited value. However, when genotypic T2DM risk was considered independently from risk in relation to covariates, it was suggested that genetic profiles might have a supplementary role related to conventional T2DM risk factors in identifying individuals at high risk of T2DM. Copyright © 2015 John Wiley & Sons, Ltd.
- Published
- 2015
45. A case of hypoglycemia attributable to atypical antipsychotic drugs
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Tsukasa Nakamaru, Katsuya Fuse, Kazuaki Morohashi, Osamu Kuwabara, Hajime Ishiguro, Ganji Kuroiwa, Kouji Sato, Hirohito Sone, Osamu Hanyu, and Satoshi Houya
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Olanzapine ,medicine.medical_specialty ,business.industry ,medicine.drug_class ,musculoskeletal, neural, and ocular physiology ,Endocrinology, Diabetes and Metabolism ,Insulin ,medicine.medical_treatment ,nutritional and metabolic diseases ,Atypical antipsychotic ,Pharmacology ,Hypoglycemia ,Organic disease ,medicine.disease ,Endocrinology ,Insulin resistance ,Internal medicine ,Diabetes mellitus ,cardiovascular system ,Internal Medicine ,medicine ,business ,Dyslipidemia ,medicine.drug - Abstract
We report a 55-year-old Japanese man who presented with symptomatic hypoglycemia attributable to atypical antipsychotic drugs (APD). He had been taking olanzapine, quetiapine, and paliperidone. One morning, his consciousness level dropped without inducement and his blood glucose was low. His symptoms improved after intravenous glucose infusion. These episodes reoccurred a few times, but ceased after APD were stopped. Physical examination did not find any organic disease that might cause hypoglycemia. Hyperglycemia, dyslipidemia, and body weight gain are well-documented side-effects of APD, but hypoglycemia is rare. There are a variety of hypotheses on how APD cause hypoglycemia: (1) Weight gain and dyslipidemia caused by APD might increase insulin resistance, meaning that excess insulin is secreted, causing hypoglycemia. (2) The quantity of basal insulin secreted by pancreatic beta cells might be enhanced by APD, which might cause hypoglycemia. (3) APD might work as an antagonist of muscarinic receptors such that insulin secretion might continue even after the glucose level has returned to normal, which might cause hypoglycemia. However, the mechanism is not fully understood and more research is needed.
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- 2015
46. Relationships among cardiorespiratory fitness, muscular fitness, and cardiometabolic risk factors in Japanese adolescents: Niigata screening for and preventing the development of non-communicable disease study-Agano (NICE EVIDENCE Study-Agano) 2
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Hitoshi Shimano, Kazuo Furukawa, Hajime Ishiguro, Kazuya Fujihara, Taeko Osawa, Hirohito Sone, Yohei Ogawa, Satoshi Matsunaga, Mariko Hatta, Masahiko Yamamoto, Sakiko Y. Morikawa, and Masahiro Ishizawa
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Male ,Mean arterial pressure ,medicine.medical_specialty ,Adolescent ,Endocrinology, Diabetes and Metabolism ,Physical fitness ,030209 endocrinology & metabolism ,Logistic regression ,03 medical and health sciences ,Grip strength ,0302 clinical medicine ,Japan ,Metabolic Diseases ,Risk Factors ,Internal medicine ,Internal Medicine ,Medicine ,Humans ,Mass Screening ,030212 general & internal medicine ,Muscle Strength ,Noncommunicable Diseases ,medicine.diagnostic_test ,business.industry ,Cardiorespiratory fitness ,Blood pressure ,Cross-Sectional Studies ,Cardiorespiratory Fitness ,Cardiovascular Diseases ,Physical Fitness ,Pediatrics, Perinatology and Child Health ,Exercise Test ,Female ,business ,Lipid profile ,Body mass index - Abstract
OBJECTIVE To examine the independent and combined associations of cardiorespiratory fitness (CRF) and muscular fitness (MF) with cardiometabolic risk factors in Japanese adolescents. METHODS A cross-sectional study including 993 Japanese adolescents (aged 13-14 years) was undertaken. Height, body mass, blood pressure, lipid profile (non-fasting), and HbA1c were measured. The physical fitness (PF) test included measurements of CRF (20 m multistage shuttle run test), upper limb strength (hand grip strength), lower limb strength (standing long jump), and muscular endurance (sit-ups). The clustered cardiometabolic risk (CCMR) was estimated by summing standardized Z-scores of body mass index (BMI), mean arterial pressure (MAP), non-high-density lipoprotein cholesterol (non-HDL-C), and HbA1c. RESULTS Linear regression analysis showed that all PF factors except for muscular endurance were inversely correlated with CCMR (P
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- 2017
47. Overt Proteinuria, Moderately Reduced eGFR and Their Combination Are Predictive of Severe Diabetic Retinopathy or Diabetic Macular Edema in Diabetes
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Taeko Osawa, Satoru Kodama, Hiruma Hasebe, Kazuya Fujihara, Nauta Yamanaka, Yasuhiro Matsubayashi, Hirohito Sone, Masanori Kaneko, Masahiko Yamamoto, Masahiro Ishizawa, Hiroyasu Seida, Hajime Ishiguro, and Shiro Tanaka
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Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Renal function ,Gastroenterology ,Macular Edema ,Young Adult ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Renal Insufficiency, Chronic ,Aged ,Proportional Hazards Models ,Glycated Hemoglobin ,Diabetic Retinopathy ,Proteinuria ,business.industry ,Proportional hazards model ,Incidence ,Incidence (epidemiology) ,Hazard ratio ,Diabetic retinopathy ,Middle Aged ,medicine.disease ,Confidence interval ,Diabetes Mellitus, Type 2 ,Female ,medicine.symptom ,business ,Follow-Up Studies ,Glomerular Filtration Rate - Abstract
Purpose Since the combined effects of proteinuria and a moderately decreased eGFR on incident severe eye complications in patients with diabetes are still largely unknown, these associations were determined in a large historical cohort of Japanese patients with diabetes mellitus. Methods We evaluated the effects of overt proteinuria (OP) (dipstick 1+ and over) and/or moderately reduced estimated glomerular filtration rate (eGFR) (MG) (baseline eGFR 30.0-54.9 mL/min/1.73 m2) on the incidence of treatment-required diabetic eye diseases (TRDED). We divided 7709 patients into four groups according to the presence or absence of OP and MG: no OP without MG (NP[MG-]), OP without MG (OP[MG-]), no OP with MG (NP[MG+]), and OP with MG (OP[MG+]). Multivariate Cox analyses were performed to calculate hazard ratios (HRs) with 95% confidence intervals for combinations of the presence and/or absence of OP and MG on the risk of developing TRDED. Results During the median follow-up period of 5.6 years, 168 patients developed TRDED. HRs for OP and MG for incident TRDED were 1.91 (95% confidence interval, 1.27-2.87) and 1.90 (1.11-3.23), respectively. HRs for incident TRDED were 1.73 (1.11-2.69) and 5.57 (2.40-12.94) for OP(MG-) and OP(MG+), respectively, in comparison with NP(MG-). Conclusions In Japanese patients with diabetes, OP and MG were separately as well as additionally associated with higher risks of TRDED. Results indicate the necessity of the simultaneous assessment of proteinuria and eGFR for appropriate evaluation of risks of severe eye complications in patients with diabetes.
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- 2019
48. Higher pulse pressure predicts initiation of dialysis in Japanese patients with diabetes
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Nauta Yamanaka, Yasuhiro Matsubayashi, Hajime Ishiguro, Mayuko Harada, Hirohito Sone, Hiroyasu Seida, Masahiro Ishizawa, Taeko Osawa, Shiro Tanaka, Masahiko Yamamoto, Hiroshi Suzuki, Satoru Kodama, Kazuya Fujihara, and Hitoshi Shimano
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Adult ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Blood Pressure ,030209 endocrinology & metabolism ,030204 cardiovascular system & hematology ,Cohort Studies ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Japan ,Renal Dialysis ,Internal medicine ,Diabetes mellitus ,Diabetes Mellitus ,Internal Medicine ,medicine ,Humans ,Dialysis ,Aged ,business.industry ,Incidence (epidemiology) ,Hazard ratio ,Middle Aged ,Prognosis ,medicine.disease ,Confidence interval ,Pulse pressure ,Blood pressure ,Arterial stiffness ,Cardiology ,Female ,business ,Follow-Up Studies - Abstract
AIMS To determine incidence and predictors of starting dialysis in patients with diabetes emphasizing blood pressure variables. METHODS A nationwide database with claim data on 18 935 people (15 789 men and 3146 women) with diabetes mellitus aged 19 to 72 years in Japan was used to elucidate predictors for starting dialysis. Initiation of dialysis was determined from claims using ICD-10 codes and medical procedures. Using multivariate Cox modelling, interactions between glycaemic and blood pressure values were determined. RESULTS During a median follow-up of 5.3 years, incidence of dialysis was 0.81 per 1000 person-years. Multivariate analysis of a model involving systolic and diastolic blood pressure (SBP and DBP) simultaneously as covariates showed that hazard ratios (HRs) for starting dialysis for each 1-SD elevation in SBP and DBP were 2.05 (95% confidence interval 1.58-2.64) and 0.66 (0.50-0.88), respectively, implying that pulse pressure (PP) was a promising predictor. For confirmation, a model involving SBP and PP simultaneously as covariates demonstrated that HRs for each 1-SD elevation in SBP and PP were 1.09 (0.81-1.48) and 1.54 (1.14-2.08), respectively, with PP the more potent predictor. Compared with HbA1c
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- 2019
49. Prevention of postprandial hypotension-related syncope by caffeine in a patient with long-standing diabetes mellitus.
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Kazuo Furukawa, Tatsuro Suzuki, Hajime Ishiguro, Hiroshi Morikawa, Keiko Sonoda, Kenichi Iijima, Masahiro Ito, Osamu Hanyu, and Hirohito Sone
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- 2020
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50. Quantitative assessment of genetic testing for type 2 diabetes mellitus based on findings of genome-wide association studies
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Hirohito Sone, Hajime Ishiguro, Yoko Yachi, Osamu Hanyu, Hitoshi Shimano, Satoru Kodama, Nobumasa Ohara, Chika Horikawa, Kazuya Fujihara, Shiro Tanaka, and Kiminori Kato
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0301 basic medicine ,Epidemiology ,MEDLINE ,030209 endocrinology & metabolism ,Genome-wide association study ,Type 2 diabetes ,Bioinformatics ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Polymorphism (computer science) ,Diabetes mellitus ,Medicine ,Humans ,Genetic Predisposition to Disease ,Genetic Testing ,Genetic testing ,medicine.diagnostic_test ,business.industry ,Type 2 Diabetes Mellitus ,medicine.disease ,030104 developmental biology ,Diabetes Mellitus, Type 2 ,Meta-analysis ,business ,Genome-Wide Association Study - Published
- 2016
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