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23 results on '"Han, Byoung-Don"'

9. Human uridine monophosphate synthase: baculovirus expression, immunoaffinity column purification and characterization of the acetylated amino terminus

Author

Han, Byoung-Don, Livingstone, Laura R., Pasek, Daniel A., Yablonski, Michael J., and Jones, Mary Ellen

Subjects
Proteins -- Synthesis, Amino acids -- Synthesis, Baculoviruses -- Research, Immunoassay -- Analysis, Biological sciences, Chemistry
Abstract

The N-terminal sequence of pure human uridine monophosphate synthase is modified in vitro and that changes involve removal of N-terminal methionine residue from the gene-coded sequence and subsequent acetylation of the formerly penultimate alanine residue to yield acetyl-AVAR as the acetylated N-terminal tetrapeptide. Purified protein expressed in cabbage looper (Tricoplusiani) larvae expressing human cDNA also begins with the N-terminal acetyl-AVAR.

Published
1995

10. Prevalence of high-risk human papillomaviruses and abnormal PAP smears among women visiting gynaecological outpatient units in Kazakhstan: A cross sectional study.

Author

Kaidarova, Dilyara, primary, Kairbayev, Murat, additional, Kim, Hong, additional, Han, Byoung-Don, additional, Sanzhar, Ismailov, additional, Shibanova, Azat, additional, Kukubassov, Yerlan, additional, Shalbayeva, Rash, additional, Yeleubayeva, Zhanar, additional, Bolatbekova, Raikhan, additional, Park, Han Jeong, additional, and Kim, Hye Jeong, additional

Published
2018
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11. Identification of hemoglobin-alpha and -beta subunits as potential serum biomarkers for the diagnosis and prognosis of ovarian cancer

Author

Cho Young-Lae, Lee Joon-Mo, Namkoong Sung-Eun, Ahn Woong-Shick, Park Sung-Pil, Kim Young-Wan, Jun Heung-Jae, Bae Su-Mi, Jin Hyun-Sun, Choi Ho-Sun, Kim Chong-Kook, Han Byoung-Don, and Nam Gye-Hyun

Subjects
Adult, Cancer Research, medicine.medical_specialty, Protein subunit, Protein Array Analysis, Enzyme-Linked Immunosorbent Assay, Biology, Sensitivity and Specificity, Mass Spectrometry, Andrology, Internal medicine, Biomarkers, Tumor, medicine, Humans, Beta (finance), Aged, Aged, 80 and over, Ovarian Neoplasms, Case-control study, Cancer, Hemoglobin A, General Medicine, Middle Aged, Prognosis, medicine.disease, Endocrinology, Oncology, Case-Control Studies, Cancer cell, Biomarker (medicine), Female, Hemoglobin, Ovarian cancer
Abstract

The development of new biomarkers for ovarian cancer is clearly necessary for the improved detection and monitoring of the disease. Surface enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) can be employed in the identification of differentially expressed proteins in cancer cells. The objective of this study was, then, to identify potential diagnostic serological biomarkers for ovarian cancer. We performed protein expression difference analyses of 45 serum samples using SELDI protein chip array. Forty-five sera obtained from ovarian cancer patients (n=35) and normal healthy females (n=10), were profiled on the surface of SELDI protein chip. The candidate biomarkers were purified by CM-Sepharose, and their N-terminal amino sequence was determined. The amounts of hemoglobin (Hb) in cancer patient's sera versus that of normal sera were measured by ELISA. Nine sera proteins that were found to be significantly differentially expressed (P

Published
2005

12. Pharmacogenetic Analysis of Pediatric Patients with Acute Lymphoblastic Leukemia: A Possible Association between Survival Rate and ITPA Polymorphism

Author

Kim, Hyery, primary, Kang, Hyoung Jin, additional, Kim, Hyo Jeong, additional, Jang, Mi Kyung, additional, Kim, Nam Hee, additional, Oh, Yongtaek, additional, Han, Byoung-Don, additional, Choi, Ji-Yeob, additional, Kim, Chul Woo, additional, Lee, Ji Won, additional, Park, Kyung Duk, additional, Shin, Hee Young, additional, and Ahn, Hyo Seop, additional

Published
2012
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14. Pharmacogenetic Study of Methotrexate and 6-Mercaptopurine Related Genes in Korean Pediatric Patients with Acute Lymphoblastic Leukemia.

Author

Kang, Hyoung Jin, primary, Seo, Young-Jin, additional, Lee, Chang Jin, additional, Kim, Hyery, additional, Jang, Mi Kyung, additional, Koh, Sang Hyeok, additional, Oh, Yongtaek, additional, Han, Byoung-Don, additional, Kim, Chul Woo, additional, Kim, Eun Kyung, additional, Shin, Hee Young, additional, and Ahn, Hyo Seop, additional

Published
2007
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15. Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients

Author

Bae, Su-Mi, primary, Min, Hyun-Jin, additional, Ding, Guo Hua, additional, Kwak, Sun-Young, additional, Cho, Young-Lae, additional, Nam, Kye-Hyun, additional, Park, Choong Hak, additional, Kim, Yong-Wan, additional, Kim, Chong-Kook, additional, Han, Byoung-Don, additional, Lee, Young-Joo, additional, Kim, Do Kang, additional, and Ahn, Woong-Shick, additional

Published
2006
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16. Cell Cycle Regulatory Protein Expression Profiles by Adenovirus p53 Infection in Human Papilloma Virus-associated Cervical Cancer Cells

Author

Lee, Yong-Seok, primary, Bae, Su-Mi, additional, Kwak, Sun-Young, additional, Park, Dong-Chun, additional, Kim, Yong-Wook, additional, Hur, Soo-Young, additional, Park, Eun-Kyung, additional, Han, Byoung-Don, additional, Lee, Young-Joo, additional, Kim, Chong-Kook, additional, Kim, Do Kang, additional, and Ahn, Woong-Shick, additional

Published
2006
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19. Immunization with Adenoviral Vectors Carrying Recombinant IL-12 and E7 Enhanced the Antitumor Immunity against Human Papillomavirus 16-associated Tumor

Author

Park, Eun-Kyung, primary, Kim, Young-Wook, additional, Lee, Joon-Mo, additional, NamKoong, Sung-Eun, additional, Kim, Do-Gang, additional, Chun, Heung-Jae, additional, Han, Byoung-Don, additional, Bae, Su-Mi, additional, Jin, Hyun-Sun, additional, Sin, Jeong-Im, additional, and Ahn, Woong-Shick, additional

Published
2005
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22. Identification of hemoglobin-α and -β subunits as potential serum biomarkers for the diagnosis and prognosis of ovarian cancer.

Author

Ahn Woong-Shick, Park Sung-Pil, Bae Su-Mi, Lee Joon-Mo, Namkoong Sung-Eun, Nam Gye-Hyun, Cho Young-Lae, Choi Ho-Sun, Jun Heung-Jae, Kim Chong-Kook, Kim Young-Wan, Han Byoung-Don, and Jin Hyun-Sun

Subjects
OVARIAN cancer, BIOMARKERS, CANCER diagnosis, CANCER prognosis, HEMOGLOBINS
Abstract

The development of new biomarkers for ovarian cancer is clearly necessary for the improved detection and monitoring of the disease. Surface enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) can be employed in the identification of differentially expressed proteins in cancer cells. The objective of this study was, then, to identify potential diagnostic serological biomarkers for ovarian cancer. We performed protein expression difference analyses of 45 serum samples using SELDI protein chip array. Forty-five sera obtained from ovarian cancer patients (n = 35) and normal healthy females (n = 10), were profiled on the surface of SELDI protein chip. The candidate biomarkers were purified by CM-Sepharose, and their N-terminal amino sequence was determined. The amounts of hemoglobin (Hb) in cancer patient's sera versus that of normal sera were measured by ELISA. Nine sera proteins that were found to be significantly differentially expressed (P < 0.05) between the sera of ovarian cancer patients and that of normal healthy females were selected using the WCX2 array. The most distinctive polypeptide peaks detected in the ovarian cancer samples were at 15.1 and 15.8 kDa and these two peaks were identified as the hemoglobin-alpha (Hb-α) and -beta (Hb-β) chain, respectively. ELISA indicated that the sensitivity for intact Hb level was 77% in sera obtained from ovarian cancer patients, as compared with normal healthy female sera. In conclusion, two ovarian cancer biomarker proteins were discovered and identified as Hb-α and Hb-β. Hb levels were significantly different in ovarian cancer serum samples and those obtained from normal healthy females, as determined by ELISA. Additional studies are required to further validate Hb-α and Hb-β biomarkers. (Cancer Sci2005; 96: 197–201) [ABSTRACT FROM AUTHOR]

Published
2005
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23. Development of DNA chip for the simultaneous detection of various beta-lactam antibiotic-resistant genes.

Author

Lee Y, Lee CS, Kim YJ, Chun S, Park S, Kim YS, and Han BD

Subjects
Bacteria genetics, Bacteria metabolism, Oligonucleotide Array Sequence Analysis methods, beta-Lactam Resistance genetics
Abstract

A robust and fast DNA chip method was developed in order to detect the various beta-lactam antibiotic-resistance genes in one slide. These genes included PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY, and AmpC. beta-lactam antibiotic-resistance genes were labeled with a fluorescent nucleotide by a multiplex polymerase chain reaction using a mixture of specific primer sets for each gene. This labeled target was hybridized with a DNA chip that contained the spots of the specific probe DNAs for each beta-lactam antibiotic-resistance gene. This technique made it possible to detect the specific resistance gene, even in a single bacterium.

Published
2002

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