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Speed reduction markings have been installed on highways as perceptual countermeasures for speeding. However, little is known about the effects of the shape and interval of road markings on driving speed and lane position. In this paper, a driving simulator experiment and questionnaires were performed to explore the effects of speed reduction marking patterns on driving speed and standard deviation of lane position (SDLP), as well as drivers’ subjective feelings, mental workload, and visual attention. Thirty-nine participants drove on a simulated two-lane rural highway where speed reduction markings with different shapes and intervals were presented at horizontal curves. The pavement markings were associated with reduced throttle values and mean speed in advance of a horizontal curve. The marking shape did not affect participants’ speed choice or SDLP. A cognitive alerting effect of the speed reduction markings was dominant because the participants did not drive more slowly with the markings with converging intervals toward the traveling direction compared to those with a constant interval. A questionnaire on drivers’ attention reflected a potential use of road markings for drivers’ lane-position maintenance. Since less than 18 % of the participants noticed the convergence in marking intervals, speed reduction markings may also induce the perceptual illusion of acceleration.

We have explored orally effective thyrotropin-releasing hormone (TRH) mimetics, showing oral bioavailability and brain penetration by structure–activity relationship (SAR) study on the basis of in vivo antagonistic activity on reserpine-induced hypothermia in mice. By primary screening of the synthesized TRH mimetics, we found a novel TRH mimetic: l-pyroglutamyl-[3-(thiazol-4-yl)-l-alanyl]-l-prolinamide with a high central nervous system effect compared with TRH as a lead compound. Further SAR optimization studies of this lead compound led to discovery of a novel orally effective TRH mimetic: 1-{N-[(4S,5S)-(5-methyl-2-oxooxazolidine-4-yl)carbonyl]-3-(thiazol-4-yl)-l-alanyl}-(2R)-2-methylpyrrolidine trihydrate (rovatirelin hydrate), which was selected as a candidate for clinical trials.

Polyethylene terephthalate (PET) fiber-reinforced polymer (FRP) composites are characterized by a large rupture strain (LRS) (usually larger than 5%) but a low elastic modulus compared to conventional FRP materials made of carbon, glass, and aramid fibers. Previous studies have proved that the use of PET FRP sheets as jacketing materials for plain concrete or reinforced concrete (RC) columns could efficiently enhance their ductility. This paper presents a combined experimental and analytical study on the shear strengthening of corroded RC columns with stirrups having different levels of corrosion before strengthening. The shear resistance contributions from the substrate column and PET FRP sheets at various volume ratios were carefully examined and their interaction mechanisms were discussed. Design equations following a conventional truss analogy were then proposed to predict the shear strength of PET FRP-wrapped columns considering the effect of stirrup corrosion, and the predictions were verified against the test results.

Road accidents between different modes of transport—such as between automobiles and pedestrians, automobiles and bicycles, or automobiles and motorcycles—are frequent. In such cases, it is important to consider the other side’s perspective. This involves the ability to correctly judge, for every given situation, how other people on the road perceive their surroundings and what they intend to do next. In this paper, we conduct two types of studies assuming that this kind of ability to consider perspectives is higher when the person is licenced to drive the mode of transport used by the other party. For Study 1, we analysed accidents involving senior citizens between the ages of 65 and 74 years, who collided with automobiles as pedestrians or cyclists (1656 and 3192 cases respectively), in terms of the accident category and type of road at the accident spot. The results indicate that possession or non-possession of a licence was irrelevant for accidents involving cyclists, but for accidents with pedestrians, senior citizens who did not possess a licence are likely to be involved in a greater number of accidents in places that require interaction with automobiles, such as while crossing at crosswalks or at intersections. For Study 2, we reviewed 875 ordinary first-class licence practical test examinees, categorised them according to their licence possession status (motorcycle licence, moped licence, or no licence), and made a category-wise comparison of the test instructor’s assessment of their ability to make a left turn. The results showed that those who had a motorcycle or moped licence tended to make a left turn more safely. Thus, the results indicate that experience with different modes of transport is likely to reduce the risk of accidents. These findings may be used to popularise educational interventions encouraging users of various transport modes to consider the perspective of others (i.e. via perspective-taking).

This paper presents research outcomes of an experimental program carried out to determine the influence of moisture and temperature on the polymer cement mortar (PCM)–concrete interface properties along with intrinsic properties of constituents. Performance of materials was evaluated using split tensile test of both composite and bulk specimens and also by observing glass transition temperature, melting point and molecular weight of extracted polymers from PCM. All specimens were exposed to different temperature conditions for temperature exposure, or wetting and drying cycles and continuous immersion in water for moisture exposure. Significant reduction in strength was observed of composite and bulk specimens with the increase in temperature, while the effect of moisture was marginal. Finally, design formulas were developed to predict the interfacial split tensile strength under various exposure conditions, by using the control tensile strength of PCM and concrete.

Summary When Escherichia coli grows in the presence of DNA-damaging agents such as methyl methanesulphonate (MMS), absence of the full-length form of Translation Initiation Factor 2 (IF2-1) or deficiency in helicase activity of replication restart protein PriA leads to a considerable loss of viability. MMS sensitivity of these mutants was contingent on the stringent response alarmone (p)ppGpp being at low levels. While zero levels (ppGpp0) greatly aggravated sensitivity, high levels promoted resistance. Moreover, M+ mutations, which suppress amino acid auxotrophy of ppGpp0 strains and which have been found to map to RNA polymerase subunits, largely restored resistance to IF2-1- and PriA helicase-deficient mutants. The truncated forms IF2-2/3 played a key part in inducing especially severe negative effects in ppGpp0 cells when restart function priB was knocked out, causing loss of viability and severe cell filamentation, indicative of SOS induction. Even a strain with the wild-type infB allele exhibited significant filamentation and MMS sensitivity in this background whereas mutations that prevent expression of IF2-2/3 essentially eliminated filamentation and largely restored MMS resistance. The results suggest different influences of IF2-1 and IF2-2/3 on the replication restart system depending on (p)ppGpp levels, each having the capacity to maximize survival under differing growth conditions.

Interview and questionnaire surveys were conducted with bus drivers in Japan, with the goal of developing an educational program for better control of emotions among bus drivers. The interviews aimed at identifying stressors and ways in which stress negatively influenced bus services. The questionnaire survey. which was being developed as a self-diagnosis tool, further provided bus drivers with the opportunity to understand their own emotional tendencies. Factor analysis identified six factors underlying work-related stress: anger at unsafe behaviours of nearby road users, irritation caused by complaints from passengers, time pressures, anxiety about traffic accidents, impatience with slow passengers, and resentment of bad-mannered passengers. The influence of stress on the drivers comprised four factors: cognitive failure, sullen behaviour, abrupt acceleration/deceleration, and aggressive driving. Moreover, drivers with lower stress were relatively older and more experienced. Based on these results, educational materials were proposed with the aim of enhancing bus drivers' understanding of their emotional processes and coping skills. Language: ja

Background: Perianal abscess (PA) is a common condition acquired in infancy, yet its treatment method remains controversial. We reviewed the outcome of neonates and young infants with PA who were treated with the traditional Japanese medicine, hainosankyuto (TJ-122). Methods: Fifteen male infants with PA under the age of 3 months were reviewed. The median age of infants at disease onset was 33 days (range, 18–88 days) and the median bodyweight was 4.1 kg (range, 2.5–6.4 kg). TJ-122 was administered at a dose of 0.20 g/kg/day (n= 13) or 0.25 g/kg/day (n= 2) orally in two or three divided doses before meals. Antibiotics were not used in any of the patients. Results: Of the 15 patients, 14 were cured and had no recurrence, with a median TJ-122 administration of 28 days (range, 14–117 days). Eight patients were cured within 28 days (53%) and 12 were cured within 60 days (80%). One patient, who was later diagnosed with growth hormone deficiency, showed incomplete healing of PA with intermittent pus discharge and recurrence. The patient was cured by 1 year of age following repeated administration of TJ-122 and juzentaihoto (TJ-48). Conclusion: Medical management with TJ-122 was effective in most neonates and young infants with PA. It appears prudent to manage these patients with hainosankyuto before resorting to surgical intervention.

Background: A nationwide survey on neonatal surgery conducted by the Japanese Society of Pediatric Surgeons has demonstrated that the mortality of neonatal intestinal perforation has risen over the past 15 years. The incidence of intestinal perforation in extremely low-birthweight (ELBW) neonates has been increasing as more ELBW neonates survive and as the live-birth rate of ELBW has increased. In contrast to necrotizing enterocolitis (NEC) and focal intestinal perforation (FIP), the pathogenesis of meconium-related ileus, defined as functional bowel obstruction characterized by delayed meconium excretion and microcolon, remains unclarified. Methods: The histology of 13 ELBW neonates with intestinal perforation secondary to meconium-related ileus was reviewed, and the radiology of 33 cases of meconium-related ileus diagnosed on contrast enema was reviewed. Specimens obtained from 16 ELBW neonates without gastrointestinal disease served as age-matched controls for histological assessment. Results: The size of the ganglion cell nucleus in meconium-related ileus and in control subjects was 47.3 ± 22.0 µm2 and 37.8 ± 11.6 µm2, respectively, which was not significantly different. In all cases of meconium-related ileus, contrast enema demonstrated a microcolon or small-sized colon, with a gradual caliber change in the ileum and filling defects due to meconium in the ileum or colon, showing not-identical locations of caliber changes and filling defects. Conclusion: Morphological immaturity of ganglia was not suggested to be the pathogenesis of meconium-related ileus. Impaction of inspissated meconium is not the cause of obstruction, but the result of excessive water absorption in the hypoperistaltic bowel before birth, although the underlying mechanism responsible for the fetal hypoperistalsis remains unclear.

Purpose To evaluate the long-term quality of life (QOL) of patients who had undergone major neonatal surgery, the psychosocial and cognitive consequences of neonatal surgical stress were assessed when the patients reached school age. Materials and methods Seventy-two patients who had undergone major neonatal surgery were enrolled in this study. Their primary diseases were anorectal malformation (ARM) in 27 cases, esophageal atresia (EA) in 23, and congenital diaphragmatic hernia (CDH) in 22. Intelligence tests using Wechsler Intelligence Scale for Children III (WISC-III) or a developmental test and the Child Behavior Checklist were conducted through questionnaires and interviews with clinical psychologists. Results Mental retardation (MR) was apparent in 25% of EA, 20% of ARM, and 18% of CDH, significantly higher than the 2% to 3% commonly found in the general population. The clinical range (CR) of the Child Behavior Checklist was seen in 35% of EA, 59% of ARM, and 38% of CDH, which is also significantly higher than the 25% typically seen in the general population. No significant differences in MR and CR were seen among the primary diseases. The most important factors influencing MR and CR remain to be identified. Conclusions To ensure true quality of life after neonatal surgical stress, pediatric surgeons must consider not only physical assessments but also cognitive, emotional, and psychosocial assessments.

本研究では，T字型無信号交差点内に敷設された台形ハンプが，非優先道路から優先道路へ右折する車両に及ぼす影響を観察調査により分析した．5つの時期（ハンプ敷設前，敷設直後，3ヶ月後，半年後，1年後）に記録された2740台のデータを分析したところ，ハンプは通行車両の速度を低下させる効果があるものの，その有効性は時間経過とともに小さくなることが示された．また，ハンプ敷設直後から3ヶ月後にかけては，ドライバーの左右確認回数が一時的に増加し，正の波及効果が認められた．その一方，一時停止率はハンプ敷設前が最も高く，負の波及効果が生じた．これらの結果から，道路安全対策の有効性評価に際しては，一時的な効果だけでなく持続的な効果測定が必要であり，また狙いとする側面以外への影響についてもリスク補償行動の観点から検証することが重要と言える．

Gastroesophageal reflux (GER) is an important sequela of congenital diaphragmatic hernia (CDH) repair. This study investigated the physiological and clinical characteristics of GER in CDH survivors.A total of 52 CDH survivors were investigated retrospectively. Esophageal acid exposure was evaluated with 24-h esophageal pH monitoring in all patients, and esophageal anatomical and motor functional abnormalities were examined with videomanometry in 16 patients.Fundoplication was necessary in 1 patient. Medical treatment with acid suppression or rikkunshito, a traditional Japanese medicine, was successful in nine patients, and the reflux symptoms were ameliorated at the age of 3 years. The percentage of total time the esophageal pH was below 4.0 (reflux index: RI) ranged from 0.1 to 44.3%. No patient with an RI10% had reflux symptoms requiring treatment. The basal lower esophageal sphincter (LES) tone ranged from 15 to 35 mmHg (median 25 mmHg). Esophageal peristalsis was preserved in all of the patients examined, except one who had failed peristalsis and poor clearance in the dilated esophagus.The motor function of the esophageal body and LES is usually preserved in CDH survivors despite the wide range of esophageal acid exposure in early infancy. Those with symptomatic GER outgrow it, unless associated with advanced respiratory distress or neurological impairment.

Summary Conversion of bacteriophage Mu repressor to ClpXP-sensitive form correlates with induced local flexibility at the ClpX recognition motif located at the C-terminal end. Changing the C-terminal valine to an alanine (RepV196A) caused the degradation tag to be constitutively active like that of mutant repressors called Vir, which have a dominant ClpXP-sensitive conformation. However, unlike Vir, RepV196A was unable to convert wild-type repressor (Rep) to the ClpXP-sensitive form. In mixtures with Rep, only RepV196A was rapidly degraded by ClpXP. Unlike Rep, RepV196A was ClpXP sensitive without induced C-terminal flexibility. And unlike adaptor proteins that tether and deliver substrates to ClpX for trans-targeting, Vir promoted rapid degradation of Rep by ClpX deleted for the tethering site that binds adaptor proteins. Therefore, Rep's ClpX recognition motif has regulable properties, allowing an alternative trans-targeting mechanism in which an inactive degradation tag is turned on by induced conformational changes.

Electronic structure of carbon nanowalls (CNW) was measured by ultraviolet photoelectron spectroscopy (UPS) in comparison with highly oriented pyrolitic graphite (HOPG). While the interlayer band of CNWs was observed at same energy of that in HOPG, the σ and π bands were observed as are in carbon nanotubes. Electronic effects by deuterium adsorption were investigated by combination of UPS and temperature programmed desorption (TPD) measurements. Deuterium adsorption preferably occurred in the boundary regions between crystallites in CNWs and enhanced the density of states associated with them.

建設コスト縮減のために，連続合成桁が見直され，様々な開発・研究が行われている．それらの設計・施工においては，床版の耐久性を向上するために，中間支点上の橋軸方向プレストレス導入を目的としたジャッキアップダウン工法や，内・外PC 鋼材を用いたプレストレス導入工法が主流となっている．しかしながら，これらの工法では，使用限界状態が改善されるものの，終局限界状態における耐荷力は全く，または，ほとんど改善され得ない．そこで，加熱した補強鋼板を鋼桁に高力ボルトで取り付け，鋼桁を補強すると同時にプレストレス力を導入する熱プレストレス工法によって，使用限界状態と同時に，終局限界状態の耐荷力を向上させる研究を試みた．本稿では，その実験，および解析結果を報告するものである．

Two asteroids 2001 QQ199 and 2004 AE9 and two comets P/LINEAR-Catalina and P/LINEAR are found to be quasi-satellites of Jupiter at present time. The Tisserand parameters of these four bodies lie between 2.3 and 2.5, which means two asteroids might be Jupiter family comets and will show a cometary’s activity in the near future.

Kinoshita and Nakai (Celest. Mech. Dyn. Astr. 75, 125–147, 1999) gave the analytical solution of the Kozai mechanism. In this solution the eccentricity and the inclination of a disturbed body take any value, but the argument of the pericenter is restricted to take 0° or 90°. In this paper, we derive the general solution that can be applied for any value of the argument of the pericenter.

近年，過去の高度経済成長期に建設された多くの道路橋が，高齢化の時期を迎えている．したがって，これらの橋梁を，貴重な社会資本として，延命化させることが重要な課題である．既設鋼橋の補強工法としては，単なる添接補強材工法と外ケーブル工法とが挙げられるが，その補強効果に，長短がある．筆者らは，既設鋼橋の新しい補強・補修工法として，熱プレストレス工法を提案してきた．この工法は，耐荷力が不足している橋梁でも，B活荷重対応橋梁として，格上げすることができる，同時に，耐久陸も向上させることができる．本文では，この熱プレストレス工法による既設連続桁の補強・補修効果について検討した結果を報告する．

Intramolecular arylation of properly designed substrates bearing a ketone, formyl, or nitro terminating group was achieved by use of a PdCl2(Ph3P)2–Cs2CO3 reaction system to form a variety of carbocyclic compounds. Arylation in ketone compounds afforded benzene-annulated bridged or spirocycloalkanone derivatives, depending on the structure of the cyclization precursors. Arylation in formyl compounds occurred at the α-position (α-arylation) or at the carbonyl carbon (carbonyl-arylation) depending on the structure of the cyclization precursors and on the reaction solvent. An α-arylated secondary nitro group was partially transformed to ketone in the manner of the Nef reaction, whereas a tertiary nitro group was partially eliminated to afford a styrene-type olefin.

Primosome assembly protein PriA functions in the assembly of the replisome at forked DNA structures. Whereas its N-terminal DNA binding domain (DBD) binds independently to DNA, the affinity of DBD protein for forked structures is relatively weak. Although the PriA helicase domain (HD) is required for high affinity fork binding, HD protein had very low affinity for DNA. It had only low levels of ATPase activity, and it hydrolyzed ATP when DNA was absent whereas PriA did not. HD catalyzed unwinding of a minimal substrate composed of a duplex with a 3' single-stranded tail. Single-strand binding protein (SSB) bound to the tail of this substrate inhibited this reaction by full-length PriA but enhanced the reaction by HD. SSB stabilized binding of PriA but not of DBD or HD to duplexes with a 5' or 3' single-stranded tail. On forked substrates SSB enhanced helicase action on the lagging-strand arm by PriA but not by HD. The results indicate that synergy of the DBD and HD allows stable binding at the interface between duplex and single-stranded DNA bound by SSB. This mode of binding may be analogous to fork binding, which orients the helicase to act on the lagging-strand side of the fork.

Thanks to the recent data obtained from the NEAR space probe, we calculate in this paper, with a precision never reached so far for an asteroid, the precession and the nutation of Eros 433. In a preliminary step, we show that Eros obliquity has a remarkable value of 89.0° which tends to align its figure axis along the orbital plane. This very specific obliquity has some consequences on the motion of the axis of figure: one is the very small amplitude of the precession in longitude, for which we get the value ψ =2″.84/ y . Moreover, we calculate Eros nutation for the figure axis due to the Sun, after developing the perturbing potential at the 4th order of the eccentricity. We show that the figure axis undergoes very large oscillations in the direction perpendicular to Eros orbital plane, due to the nutation in obliquity. Peak to peak, these oscillations reach 55″, which is far larger than the amplitudes of the nutations of the Earth due to the Sun (of the order of 2″). Moreover, we give the analytical developments of Δψ and Δe, both for the axis of angular momentum, and the axis of figure.

Toriello-Carey syndrome comprises agenesis of the corpus callosum, telecanthus, small palpebral fissures, Pierre Robin sequence, abnormal ears, and cardiac defects. We report a boy who has some additional findings, including a severe respiratory failure and intestinal dysmotility. The boy died of these two disorders at age 13 months. Histological examination revealed pulmonary immaturity and a defect of smooth muscle cells in the longitudinal muscle coat of the intestinal musculature, both of which might explain some aspects of the pathophysiology of the patient.

Der Zustand der Bauwerke einer Stadtstrase verschlechtert sich schnell unter ungunstigen Umweltbedingungen, dem Anwachsen des Schwerlastanteils und dem zunehmenden Lebensalter. Bei der Wartung dieser Bauwerke sind viele Probleme zu losen: So reihen sich uber die ganze Lange altere und neuere Konstruktionen aneinander. Eine notwendige Komplettsanierung eines Strasenabschnitts wird damit schwierig, noch dazu unter dem gegenwartigen langsamen Wachstum der japanischen Wirtschaft. Die Sperrung einer Fahrbahn aufgrund konstruktiver Schaden wurde das Verkehrsnetz zum Erliegen bringen und zu langer andauernder wirtschaftlicher Stagnation in der Region fuhren. Unter diesen Umstanden analysiert dieser Beitrag, inwieweit eine vernunftige Beurteilung der Schadstellen hinsichtlich ausgesuchter Schadensbilder moglich ist. Daneben werden effektive Reparatur- und Instandsetzungsmasnahmen empfohlen, so das wichtige Bauwerke dauerhaft in gutem Zustand bewahrt werden konnen.

Conformational studies of potent and selective endothelin-A (ET(A)) receptor antagonists, 4-substituted (R)-2-(benzo[1,3]-dioxol-5-yl)-6-isopropoxy-2H-chromene-3-carboxylic acids, are reported. X-ray crystallography and NMR studies of the 4-anisyl derivative 2 (S-1255), the stable atropisomers 3 and the 4-n-butyl derivative 4 reveal that the A-, B- and C-rings in these compounds adopt a L-like conformation in both solution and solid states. Molecular mechanics calculation shows that this L-like conformation is an inevitable conformation as determined by intramolecular steric repulsions. These 2H-chromene derivatives bound to an ET(A) receptor with IC(50) values of less than 1 nM, whereas the dihydro compounds 7 and 9 not having the L-like conformation showed weaker affinities. These results suggest that the L-like conformation is specifically recognized by the active site of the ET(A) receptor. The roles of the L-like conformation in the receptor binding are discussed.

Novel 15-membered macrolides possessing the dilactone skeleton, diolides 13a and 13b, have been synthesized in our research program aimed at finding new antibacterial macrolides. Key strategic elements of the approach include the ring-expanding reaction of 13-membered dilactones, prepared from erythromycin A (Ery-A), to 15-membered dilactones via intramolecular translactonization. The absolute configuration at the regenerated C-8 position of the new diolides was determined by chemical transformation, leading to the corresponding lactam analogues, whose stereochemistry is known in the literature. For further confirmation, X-ray analysis was performed. The X-ray structure determination of 13a revealed a backbone conformation similar to that of Ery-A. Novel 15-membered diolide 13a and the 11,12-diol 18 exhibited antibacterial activities comparable to that of Ery-A.

Background/Purpose: There are few follow-up studies comparing posterior sagittal anorectoplasty (PSARP) with conventional procedures for patients with anorectal malformations (ARM). The authors have examined retrospectively postoperative anorectal function of patients with ARM treated with PSARP compared with those treated with conventional methods. Methods: Anorectal function in 23 patients with high and intermediate type anorectal malformations (PSARP group), who underwent PSARP more than 4 years previously, were assessed by Kelly's clinical scoring system and objective studies. These results were compared with those in 14 cases (5 high and 9 intermediate type cases; control group), who underwent other conventional surgical procedures. Results: Using Kelly's clinical scoring system, scores of the PSARP group compared with the control group were good in 48% versus 21%, fair in 48% versus 58%, and poor in 4% versus 21%, respectively. Barium enema studies suggested better anorectal sphincteric function in patients with high anorectal malformation in the PSARP group. Magnetic resonance imaging (MRI) studies showed more correct placement of the rectum through the striated muscle complex in the PSARP group at the I-line level. Manometric studies showed no difference in maximum resting pressure, anal canal length, and the incidence of anorectal reflex between the two groups. Conclusions: The favorable results of MRI and barium enema studies can be explained by direct visualization of the striated muscle complex with the aid of electrical stimulation as well as no harmful effects of amputation of the sphincter muscle in PSARP. However, manometric studies suggest anorectal function in patients with high and intermediate anorectal malformations is limited even after PSARP. Long-term postoperative follow-up with adequate bowel management is required for all patients with high or intermediate anorectal malformation. J Pediatr Surg 37:1529-1533. Copyright 2002, Elsevier Science (USA). All rights reserved.

We carry out simulations to investigate the dynamics of the HD 82943 planetary system with two resonant Jupiter-like planets, and to reveal possible stabilizing mechanism for the system. By following different coplanar configurations in the neighborhood of the best-fit orbits, we find that all the stable cases are involved in the 2:1 mean motion resonance and that the alignment of the periastra of the two planets also plays important part in the secular orbital evolution, indicating that these two kinds of mechanisms could be responsible for the dynamics of the system under study.

To trigger bacteriophage Mu transposition and replication in response to physiological signals, its immunity repressor must be synchronously inactivated. Two repressor mutants (Vir), which have an altered C-terminal domain and are highly susceptible to degradation by ClpXP protease, confer a dominant negative phenotype by promoting degradation of the wild-type repressor. To search for other modified repressors that can induce Mu derepression in vivo and to determine what part of the inducing repressor molecules are needed to target the unmodified repressor population, repressor peptides with nested deletions starting at the C-terminal end were constructed. Such peptides with a C-terminal ssrA degradation tag promoted a sharp reduction in cellular levels of full-length unmodified repressor, a process largely dependent upon the clpP protease function. Only the repressor DNA-binding domain, located at the N-terminal end, was required in tagged peptides to target unmodified repressor. In addition, some repressor peptides containing the DNA-binding domain promoted derepression without the clpP function, being able to promote repressor inactivation without promoting its degradation. None of the modified repressors could promote derepression if immunity was established by a mutant repressor lacking 18 residues at its C-terminal end. The results indicate that inducing repressor peptides influence the function of the C-terminal domain of the intact repressor, a domain that regulates its degradation and DNA binding. They suggest the possibility that tagged repressor molecules, produced by stalled ribosomes, can be inducers of transposition under starvation conditions.

Background. This study evaluated zoom endoscopic findings according to four components in comparison with the histologic findings of acute cellular rejection (ACR) in a living-related small bowel transplantation (SBTx) patient. Methods. A 16-year-old boy with microvillus inclusion disease underwent SBTx with a 150-cm-long ileal graft from a blood-identical living-related donor. The endoscope was inserted into the distal stoma of the graft, and the mucosal architecture was observed under zoom. The observed findings were expressed by the following four components and graded as 0 to 2: H, homogeneity of mucosal change, minimal (H-0), patchy (H-1), diffuse (H-2); V, appearance of villi, stringlike (V-0), tonguelike (V-1), domelike (V-2); W, widening of crypt area, narrow (W-0), slightly widened within a width of one villus (W-1), markedly widened beyond a width of one villus (W-2); E, erythema in crypt area: no redness (E-0), sporadic erythema (E-1), diffuse erythema (E-2). Results. Histologic ACR was grade 0 in 27, grade 1 in 6, grade 2 in 3, and grade 3 in 0 occasions during 11 months after SBTx. In grade 0, 1, and 2 histology, H-0/ H-1/H-2 was 85.2%/14.8%/0%, 33.3%/66.7%/0%, and 0%/ 100%/0%, respectively, with a significant difference among the groups by grade (P

Two novel compounds, stachyflin and acetylstachyflin, have been isolated by solid-state fermentation of Stachybotrys sp. RF-7260. The structures of both metabolites, determined by detailed NMR analyses and X-ray crystallographic analysis, are novel with a pentacyclic moiety including cis-fused decalin. The absolute stereochemistry of stachyflins was determined by circular dichroism analysis. Stachyflin showed antiviral activity against influenza A virus (H1N1) in vitro with an IC50 value of 0.003 microM. Acetylstachyflin was about 77-fold less active than stachyflin.