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24 results on '"Hong, Guo-Lin"'

18. Potentiation of NMDA-Mediated Responses by Amyloid-β Peptide 1-40 in Rat Sympathetic Preganglionic Neurons

Author

Hsun-Hsun Lin, Hong-Guo Lin, Chih-Chia Lai, and Hsuan Lo

Subjects
0301 basic medicine, Pharmacology, Receptors, Ionotropic Glutamate, Cardiovascular System, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, GABA receptor, Receptors, GABA, medicine, Excitatory Amino Acid Agonists, Animals, Protein Kinase Inhibitors, Protein kinase C, Protein Kinase C, Neurons, Amyloid beta-Peptides, Ganglia, Sympathetic, Chemistry, General Neuroscience, Glutamate receptor, Excitatory Postsynaptic Potentials, Long-term potentiation, Biological Transport, General Medicine, Spinal cord, Peptide Fragments, Rats, Psychiatry and Mental health, Clinical Psychology, 030104 developmental biology, medicine.anatomical_structure, Phosphoserine, NMDA receptor, Geriatrics and Gerontology, 030217 neurology & neurosurgery, Ionotropic effect
Abstract

The abnormal accumulation of amyloid-β peptides (Aβ) is one of the main characteristics of Alzheimer's disease (AD). Cerebro- and cardiovascular diseases may be the risk factors for developing AD. The effect of Aβ on central sympathetic control of cardiovascular function remains unclear. The present study examines the acute effects of Aβ oligomers on the function of NMDA receptors, a subtype of ionotropic glutamate receptors, in rat sympathetic preganglionic neurons (SPNs). In the in vitro electrophysiological study, Aβ1-40 but not Aβ1-42 applied by superfusion for 5 min significantly potentiated NMDA-induced depolarizations in SPNs of neonatal rat spinal cord slice preparation. Application of Aβ1-40 had little effects on AMPA-induced depolarizations or GABA-induced hyperpolarizations. Treatment with a selective protein kinase C (PKC) inhibitor applied together with Aβ1-40 blocked the augmentation by Aβ1-40 of NMDA-induced depolarizations. Western blot analysis showed an increase in the levels of phosphoserine 896, selectively regulated by PKC, without significant changes in phosphoserine 897 on GluN1 subunits in lateral horn areas of spinal cord slices following treatment with Aβ1-40. In the in vivo study, intrathecal injection of Aβ1-40 (0.2 nmol) potentiated the pressor effects induced by NMDA (2 nmol) injected intrathecally in urethane-anesthetized rats. These results suggest that different fragments of Aβ may have differential effects on the NMDA receptor function and the selective augmentation of NMDA receptor function by Aβ1-40 may involve PKC-dependent mechanisms in sympathetic preganglionic neurons.

Published
2019

23. Sensitive and selective nitrite assay based on fluorescent gold nanoclusters and Fe 2+ /Fe 3+ redox reaction.

Author

Deng HH, Huang KY, Zhang MJ, Zou ZY, Xu YY, Peng HP, Chen W, and Hong GL

Subjects
3-Mercaptopropionic Acid chemistry, Biosensing Techniques, Fluorescence, Fluorescent Dyes chemistry, Food Analysis instrumentation, Gold chemistry, Limit of Detection, Nitrites chemistry, Oxidation-Reduction, Pork Meat analysis, Sensitivity and Specificity, Serum Albumin, Bovine chemistry, Spectrometry, Fluorescence, Food Analysis methods, Iron chemistry, Nanostructures chemistry, Nitrites analysis
Abstract

In recent years, gold nanoclusters (AuNCs) have received considerable attention as optical transducers in chemo/biosensors. Herein, a facile and efficient assay for NO 2 - has been successfully developed based on the fluorescence quenching of AuNCs co-modified by bovine serum albumin and 3-mercaptopropionic acid (BSA/MPA-AuNCs). In the presence of NO 2 - under acidic conditions, Fe 2+ can be readily oxidized and transformed to Fe 3+ , which can significantly suppress the fluorescence of BSA/MPA-AuNCs via non-radiative electron-transfer mechanism. The linear range and detection limit for this system were found to be 5-30 μM (r = 0.9975) and 0.7 μM, respectively. Other common anions and cations showed only very minor interference with the NO 2 - detection. Furthermore, the effectiveness of the proposed sensing strategy was validated by the demonstration of good performance in the determination of the amount of NO 2 - in ham samples, rendering it a powerful tool for the assessment of food security and water quality., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Published
2020
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24. PRSS1_p.Leu81Met mutation results in autoimmune pancreatitis.

Author

Gao F, Li YM, Hong GL, Xu ZF, Liu QC, He QL, Lin LQ, and Weng SH

Subjects
Adult, Aged, Amylases blood, Autoimmune Diseases blood, Autoimmune Diseases diagnosis, Autoimmune Diseases drug therapy, Autoimmune Diseases enzymology, Biomarkers blood, Biopsy, Calcium metabolism, Case-Control Studies, Female, Genetic Predisposition to Disease, Glucocorticoids therapeutic use, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Pancreatic Neoplasms enzymology, Pancreatic Neoplasms genetics, Pancreatitis blood, Pancreatitis diagnosis, Pancreatitis drug therapy, Pancreatitis enzymology, Pancreatitis, Chronic enzymology, Pancreatitis, Chronic genetics, Phenotype, Recombinant Proteins metabolism, Tomography, X-Ray Computed, Trypsin blood, Autoimmune Diseases genetics, Mutation, Pancreatitis genetics, Trypsin genetics
Abstract

Aim: To describe protease serine 1 (PRSS1) gene mutations in patients with autoimmune pancreatitis (AIP) and the clinical features of AIP., Methods: Fourteen patients with AIP, 56 with other chronic pancreatitis, 254 with pancreatic cancer and 120 normal controls were studied. The mutations and polymorphisms of four genes involved with pancreatitis or pancreatic cancer, PRSS1, SPINK1, CFTR and MEN1, were sequenced. The pathogenic mechanism of AIP was investigated by comparing the wild-type expression system with the p.81Leu→Met mutant expression system., Results: Two novel mutations (p.81Leu→Met and p.91Ala→Ala) were found in PRSS1 gene from four patients with AIP. PRSS1_p.81Leu→Met mutation led to a trypsin display reduction (76.2%) combined with phenyl agarose (Ca(2+) induced failure). Moreover, the ratio of trypsin/amylase in patients with AIP was higher than in the patients with pancreatic cancer and other pancreatitis. A large number of lymphocytes and plasma cells were found in the bile ducts accompanied by hyperplasia of myofibroblasts., Conclusion: Autoimmune pancreatitis may be related to PRSS1 gene mutations.

Published
2013
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