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3 results on '"Hybrid chitosan-gelatin hydrogel"'

1. 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation

Author

Re, F, Sartore, L, Moulisova, V, Cantini, M, Almici, C, Bianchetti, A, Chinello, C, Dey, K, Agnelli, S, Manferdini, C, Bernardi, S, Lopomo, N, Sardini, E, Borsani, E, Rodella, L, Savoldi, F, Paganelli, C, Guizzi, P, Lisignoli, G, Magni, F, Salmeron-Sanchez, M, Russo, D, Re F., Sartore L., Moulisova V., Cantini M., Almici C., Bianchetti A., Chinello C., Dey K., Agnelli S., Manferdini C., Bernardi S., Lopomo N. F., Sardini E., Borsani E., Rodella L. F., Savoldi F., Paganelli C., Guizzi P., Lisignoli G., Magni F., Salmeron-Sanchez M., Russo D., Re, F, Sartore, L, Moulisova, V, Cantini, M, Almici, C, Bianchetti, A, Chinello, C, Dey, K, Agnelli, S, Manferdini, C, Bernardi, S, Lopomo, N, Sardini, E, Borsani, E, Rodella, L, Savoldi, F, Paganelli, C, Guizzi, P, Lisignoli, G, Magni, F, Salmeron-Sanchez, M, Russo, D, Re F., Sartore L., Moulisova V., Cantini M., Almici C., Bianchetti A., Chinello C., Dey K., Agnelli S., Manferdini C., Bernardi S., Lopomo N. F., Sardini E., Borsani E., Rodella L. F., Savoldi F., Paganelli C., Guizzi P., Lisignoli G., Magni F., Salmeron-Sanchez M., and Russo D.

Abstract

Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

Published
2019

2. 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation

Author

Domenico Russo, Luigi Fabrizio Rodella, Fabio Savoldi, Cristina Manferdini, Gina Lisignoli, Kamol Dey, Luciana Sartore, Manuel Salmerón-Sánchez, Silvia Agnelli, Simona Bernardi, Vladimira Moulisova, Corrado Paganelli, Andrea Bianchetti, Fulvio Magni, Nicola Lopomo, Emilio Sardini, Federica Re, Camillo Almici, Marco Cantini, Elisa Borsani, Clizia Chinello, Pierangelo Guizzi, Re, F, Sartore, L, Moulisova, V, Cantini, M, Almici, C, Bianchetti, A, Chinello, C, Dey, K, Agnelli, S, Manferdini, C, Bernardi, S, Lopomo, N, Sardini, E, Borsani, E, Rodella, L, Savoldi, F, Paganelli, C, Guizzi, P, Lisignoli, G, Magni, F, Salmeron-Sanchez, M, and Russo, D

Subjects
food.ingredient, human platelet lysate, Biomedical Engineering, Medicine (miscellaneous), Adipose tissue, macromolecular substances, 02 engineering and technology, Gelatin, lcsh:Biochemistry, Biomaterials, human mesenchymal stem cells, 03 medical and health sciences, food, bone regeneration, Tissue engineering, human mesenchymal stem cell, medicine, lcsh:QD415-436, Mesenchymal stem cell proliferation, Bone regeneration, 030304 developmental biology, 0303 health sciences, Hybrid chitosan-gelatin hydrogel, tissue engineering, Chemistry, Mesenchymal stem cell, technology, industry, and agriculture, 021001 nanoscience & nanotechnology, 3. Good health, Cell biology, carbohydrates (lipids), medicine.anatomical_structure, Self-healing hydrogels, Original Article, Bone marrow, 0210 nano-technology
Abstract

Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

Published
2019

3. 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation.

Author

Re, Federica, Sartore, Luciana, Moulisova, Vladimira, Cantini, Marco, Almici, Camillo, Bianchetti, Andrea, Chinello, Clizia, Dey, Kamol, Agnelli, Silvia, Manferdini, Cristina, Bernardi, Simona, Lopomo, Nicola F., Sardini, Emilio, Borsani, Elisa, Rodella, Luigi F., Savoldi, Fabio, Paganelli, Corrado, Guizzi, Pierangelo, Lisignoli, Gina, and Magni, Fulvio

Subjects
*HUMAN stem cells, *MESENCHYMAL stem cells, *ADIPOSE tissue physiology, *CELL proliferation, *HYDROGELS, *CELLULAR control mechanisms
Abstract

Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells. [ABSTRACT FROM AUTHOR]

Published
2019
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