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12. 90th Annual Convention Poster Presentations and Abstracts

Author

Ellis, C. N., Coyle, D. J., Boggs, H. W., Slagle, G. W., Cole, P. A., Kuramoto, S., Ihara, O., Oohara, T., Nichols, J., Opelka, F., Gathright, J. B., Green, J. B., Poulard, J. B., Ott, A., Bank, S., Margolis, I. B., Meagher, A., Stuart, M., Heine, J. A., Rothenberger, D. A., Nemer, F. D., Christenson, C. E., Saad, R. C., Church, J. M., Fazio, V. W., Lavery, I. C., Oakley, J. R., Milsom, J. W., Schroeder, T. K., Påhlman, L., Frykholm, G., Glimelius, B., Kashtan, H., Papa, M., Wilson, B., Stern, H., Zelnick, R., Haas, P., Ajlouni, M., Fox, T., Szilagy, E., Cummings, B. J., Fleshman, J. W., Dreznick, Z., Fry, R. D., Kodner, I. J., Perry, R. E., Pemberton, J. H., Litchy, W. L., Ferrara, A., Levin, K. E., Hanson, R. B., Cali, R. L., Blatchford, G. J., Thorson, A. G., Christenson, M. A., Pitsch, R. M., Jensen, L. L., Lowry, A. C., Keighley, M. R. B., Oya, M., Oritz, J., Pinho, M., Asperer, J., Chattaphaday, G., Baeten, C., Konsten, J., Spaans, F., Soeters, P., Habets, A., Schouten, W. R., Ruseler van Embden, J. G. H., Auwerda, J. J. A., Sagar, P. M., Goodwin, P., Holdsworth, P. J., Johnston, D., Bundy, C. A., Jacobs, D. M., Bubrick, M. P., Kashiwagi, H., Konishi, F., Kanazawa, K., Woodland, D. O., Saclarides, T. J., Bapna, M. S., Kubota, Y., Sunouchi, K., Ono, M., Muto, T., Masaki, T., Suzuki, K., Adachi, M., Wong, W. D., Goldberg, S. M., Wexner, S. D., Daniel, N., Jagelman, D. G., Christiansen, J., Rasmussen, O., Zhu, B. -W., Williams, J. G., Schottler, J. L., Heyman, S., Marchetti, F., Timmcke, A. E., Hicks, T. C., Ray, J. E., Bernstein, M. A., Madoff, R. D., Caushaj, P. F., Zarbo, R. J., Ma, C. K., Shida, H., Yamamoto, T., Machida, T., Imanari, T., Wang, J. Y., You, Y. T., Tang, R. P., Chen, J. S., Chang-Chien, C. R., Sugihara, K., Hojo, K., Moriya, Y., Hasegawa, H., Krueger, B., Warren, W., Faber, L. P., Abel, M. E., Chiu, Y. S. Y., Russell, T. R., Volpe, P. A., Frazee, R. C., Roberts, J., Symmonds, S., Snyder, S., Hendricks, J., Smith, R., Merchant, N., Hashmi, H., Scalea, T., Whelan, R., Longo, W. E., Gusberg, B. J., Ballantyne, G. H., Davidson, T., Allen-Mersh, T. G., Gazzard, B., Miles, A. J. G., Wastell, C., Viponde, M., Stotter, A., Miller, R. F., Fieldman, N., Slack, W. W., Tjandra, J., Savoca, P. E., Flannery, J. T., Modlin, I. M., Tsukada, K., Tazawa, K., Lavery, E. C., Voeller, G. R., Bunch, G., Britt, L. G., Neto, J. A. Reis, Quilici, F. A., Cordeiro, F., Reis, Jr, J. A., Wojcik, J. B., Banerjee, S. R., Walters, D. L., Cherry, D. A., Bleday, R., Pena, J. P., Buls, J. G., Pascual, R., Tripodi, G., Padmanabhan, A., Schouter, W. R., Blankensteijn, J. D., Moenning, S., Huber, P., Simonton, C., Odom, C., Kaplan, E., Nightengale, S., Shah, P. C., Hashami, H. F., Kottmeier, P., Velcek, F., Klotz, D., Whelan, R. L., Sher, M. E., Bauer, J. J., Gelernt, I., Launer, D. P., Gerber, A., Nogueras, J. J., Finne, C. O., Sohn, N., Weinstein, M. A., Lugo, R. N., Eisenberg, M. M., Tsao, J., Galandiuk, S., Tuckson, W. B., Strong, S., Oakey, J. R., Ambroze, W. L., Dozois, R. R., Carpenter, H. A., Kartheuser, A. H., LaRusso, N. F., Wiesner, R. H., Ilstrup, D. M., Schleck, C. D., Ambroze, W., Beart, R., Dozois, R., Wolff, B., Pemberton, J., Kelly, K., Devine, R., Nivatvongs, S., Metzger, P., Phillips, S. F., Zinmeister, A. R., Pezim, M. E., Vignati, P., Cohen, J., Stahl, T. J., Roberts, P. L., Schoetz, Jr., D. J., Murray, J. J., Coller, J. A., Veidenheimer, M. C., Yamazaki, Y., Ribeiro, M. B., Sachar, D., Heimann, T. M., Aufses, A. H., Greenstein, A. J., Stryker, S. J., Green, D., McLeod, R. S., Cohen, Z., Cullen, J., Greenberg, G. R., Ho, C. S., Reznick, R., Wolff, B. G., Cangemi, J., Carryer, P., Jeejeebhoy, K. N., MacCarty, R., Weilland, L., Senagore, A. J., MacKeigan, J. M., Guillem, J., Ondrula, D. P., Prasad, M. L., Nelson, R. L., Abcarian, H., Coughlin, R. J., Corman, M. L., Prager, E. D., Borison, D. I., Bloom, A. D., Pritchard, T. J., McGannon, E., Sivak, M. V., van Stolk, R., Hull-Boiner, S., Milson, J. W., Sullivan, M., Rosato, G. O., Jorge, J. M., Durdey, P., Kennedy, M. J., Oster, M., Murray, J., Cirocco, W. C., Rusin, L. C., Brown, A. C., Reilly, J. C., Cataldo, P., Luchtefeld, M. A., Mazier, W. P., Wolkomir, A. F., Ruiz-Moreno, F., Alvarado-Cerna, R., Rodriguez, U., Amaro, J., Kerner, B. A., Oliver, G. C., Eisenstat, T. E., Rubin, R. J., Salvati, E. P., Dominguez, J. M., Coon, J. S., Weinstein, R. S., Kameyama, M., Fukuda, I., Imaoka, S., Iwanga, T., Kyzer, S., Mitmaker, B., Gordon, P. H., Wang, E., Grace, R. H., Gibbons, P., Scott, K. M. W., Berger, A., Mischinger, H. J., Arian-Schad, K., Davis, M., Miller, D., Fielding, L. P., Begin, L. R., Bell, A. M., Shafik, A., Abdel-Moneim, K., Khalid, A., Devine, R. M., Beart, Jr., R. W., Melton, L. J., Ngoi, S. S., Chia, J., Goh, P., Sim, E., Godwin, P., Quirke, P., Barrett, R. C., Koltun, W. A., Smith, R. J., Loehner, D., Roberts, P., Veidenheimer, M., Schoetz, D., Chattopadhyay, G., Kumar, D., Hosie, K., Kmiot, W., Mostaf, A., Tulley, N., Harding, I., Falcone, R. E., Wanamaker, S., Santanello, S. A., Carey, L. C., Rivera, D. E., Durdley, P., Gross, P. T., Sarles, J. C., Arnaud, A., Sielezneff, I., Orsoni, P., Joly, A., Limberg, B., Stolfi, V. M., Lavery, I., Oakley, J., Church, J., Fazio, V., Asbun, H. J., Castellanos, H., Asbun, J., Franko, E. R., Ivatury, R. R., Schwalb, D., Saad, R., Schroeder, T., Reis, Jr., J. A., Dziki, A. J., Duncan, M. D., Harmon, J. W., Saini, N., Malthaner, R. A., Fernicola, M. T., Hakki, F. Z., Trad, K. S., Ugarte, R. M., Ryan, P., Chang, H. R., Chavoshan, B., Barsoum, G., Bonardi, R., Scaramelo, A., Possebon, A., Peres, C., Röhrig, C., Kappas, A. M., Ortiz, J., Fan, H. A., Milsom, J., Lechner, P., Lind, P., Cesnik, H., Venkatesh, K. S., Larson, D. M., Morrison, D. N., Ramanujam, P. J., Rubbini, M., Mascoli, F., Mari, C., Bresadola, V., and Donini, I.

Published
1991
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18. Isolation of murine and human homologues of the fission-yeast dis3+ gene encoding a mitotic-control protein and its overexpression in cancer cells with progressive phenotype

Author

Lim J, Kuroki T, Kouichi Ozaki, Kohsaki H, Yamori T, Tsuruo T, Nakamori S, Imaoka S, Endo M, and Nakamura Y

Subjects
Exosome Multienzyme Ribonuclease Complex, Sequence Homology, Amino Acid, Molecular Sequence Data, Mitosis, Adenocarcinoma, Neoplasm Proteins, Fungal Proteins, Gene Expression Regulation, Neoplastic, Mice, Exoribonucleases, Tumor Cells, Cultured, Animals, Humans, Amino Acid Sequence, RNA, Neoplasm, Schizosaccharomyces pombe Proteins, Cloning, Molecular, Neoplasm Metastasis, Colorectal Neoplasms, Sequence Alignment
Abstract

To investigate genes involved in metastasis, we used a differential display method to compare the levels of gene expression in three cell lines derived from murine colon-adenocarcinoma 26 that show different metastatic potentials. The results, and subsequent Northern analyses, confirmed that one gene was expressed most strongly in NL17, the cell line with the highest experimentally metastatic potential to the lung; strongly in NL22, the line with moderately metastatic potential; and very weakly in NL4, which has no metastatic potential in recipient mice. Using this fragment as a probe, we isolated the murine cDNA as well as its human homologue and determined their DNA sequences. The cDNA sequences from both species contained open reading frames of 2874 nucleotides, encoding peptides of 958 amino acids with calculated molecular weights of approximately 109,000; the murine and human nucleotide sequences were 90% identical. The deduced amino acid sequences of these cDNAs revealed significant homology (45% identity) to the dis3+ gene product of Schizosaccharomyces pombe, a protein thought to be essential for mitotic control in the yeast. We therefore termed the murine and human genes hmc (homologue to the mitotic-control gene) and HMC, respectively. In 7 of 13 patients with colorectal cancers and liver metastases, expression of HMC was increased up to 38-fold in primary tumors and metastatic foci as compared to adjacent normal colorectal mucosa. An increase in expression of HMC, its novel product likely to belong to a structurally distinct family of mitotic-control proteins, may be associated with malignant phenotypes of some colorectal cancers.

Published
1997

19. Detection of breast cancer micrometastases in axillary lymph nodes by means of reverse transcriptase-polymerase chain reaction. Comparison between MUC1 mRNA and keratin 19 mRNA amplification

Author

Noguchi, S., Aihara, T., Motomura, K., Inaji, H., Imaoka, S., and Koyama, H.

Subjects
Base Sequence, Molecular Sequence Data, Mucin-1, Breast Neoplasms, RNA-Directed DNA Polymerase, Polymerase Chain Reaction, Sensitivity and Specificity, Lymphatic Metastasis, Axilla, Humans, Keratins, Female, Lymph Nodes, RNA, Messenger, skin and connective tissue diseases, Research Article, DNA Primers, Retrospective Studies
Abstract

Usefulness of MUC1 mRNA and keratin 19 mRNA as a target of reverse-transcriptase polymerase chain reaction (RT-PCR) was compared in the detection of breast cancer micrometastases in axillary lymph nodes. RT-PCR amplification of MUC1 mRNA and keratin 19 mRNA was conducted using total RNA samples. RT-PCR products were stained with ethidium bromide and analyzed by agarose gel electrophoresis. Expression of both MUC1 mRNA and keratin 19 mRNA was detected by RT-PCR in a breast cancer cell line (MRK) and in all the 23 primary breast cancers but not in the control lymph nodes obtained from patients with benign diseases. A serial dilution study of MRK cells against normal lymph node cells has shown that detection sensitivity of MUC1 RT-PCR and keratin 19 RT-PCR were 1/10(5) and 1/10(6) (cancer/lymph node cells), respectively. Sixty-three axillary lymph nodes were obtained from 23 patients with primary breast cancer, and metastases in each lymph node were investigated by histological examination (hematoxylin and eosin sections) and RT-PCR method. In all 10 lymph nodes, which were histologically metastasis-positive, both MUC1 mRNA and keratin mRNA were detected by RT-PCR. Of the 53 histologically negative lymph nodes, 3 (6%) and 5 (9%) lymph nodes were found to express MUC1 mRNA and keratin 19 mRNA, respectively, indicating the presence of micrometastases which could be detected by RT-PCR but not by histological examination. These results demonstrate the usefulness of both MUC1 RT-PCR and keratin 19 RT-PCR in the detection of breast cancer micrometastases in lymph nodes, and also indicate the superiority of keratin 19 RT-PCR over MUC1 RT-PCR because of its higher detection sensitivity.

Published
1996

21. Synchronous esophageal and renal cell carcinoma

Author

Kobayashi, S., primary, Kabuto, T., additional, Doki, Y., additional, Yamada, T., additional, Miyashiro, I., additional, Murata, K., additional, Hiratsuka, M., additional, Kameyama, M., additional, Ohigashi, H., additional, Sasaki, Y., additional, Ishikawa, O., additional, and Imaoka, S., additional

Published
2008
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33. Synchronous esophageal and renal cell carcinoma

Author

Kobayashi, S., primary, Kabuto, T., additional, Doki, Y., additional, Yamada, T., additional, Miyashiro, I., additional, Murata, K., additional, Hiratsuka, M., additional, Kameyama, M., additional, Ohigashi, H., additional, Sasaki, Y., additional, Ishikawa, O., additional, and Imaoka, S., additional

Published
2000
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38. A356 LIDOCAINE METABOLISM IN VIVO

Author

Oda, Y., primary, Nakamoto, T., additional, Imaoka, S., additional, Mizutani, K., additional, Tanaka, K., additional, Hase, I., additional, Nishi, S., additional, Funae, Y., additional, and Asada, A., additional

Published
1997
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