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This study investigates the mycelial biomass production and chitosan extraction potential of various Basidiomycota strains, including Heterobasidion annosum, Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, and Lentinus lepideus. Both submerged fermentation (SF) and solid-state fermentation (SSF) methods were employed. The chitosan yield in basidiocarps of Pleurotus ostreatus, Agaricus bisporus, and Ganoderma applanatum was also evaluated as a reference material. The chitosan extracted from fungal cells was characterized using elemental analyses and FTIR spectroscopy. Among the cultivated strains, P. chrysosporium exhibited the highest mycelial biomass concentration in SF (1.03 g 100 mL–1) after 14 days, while T. versicolor achieved the highest biomass concentration in SSF (3.65 g 100 mL–1). The highest chitosan yield was obtained from the mycelium of P. chrysosporium (0.38%) and T. versicolor (0.37%) in shaken SF. Additionally, commercially cultivated A. bisporus demonstrated the highest chitosan yield in fungal fruiting bodies (1.7%). The extracted chitosan holds potential as a functional biopolymer additive for eco-friendly materials, serving as an alternative to synthetic wet and dry strength agents in packaging materials. [ABSTRACT FROM AUTHOR]

The present research investigated bio-based construction material based on hemp shives and gypsum. Low-density material was developed with densities of 200, 300, and 400 kg/m3. Simple yet robust production technology was used. For the first time, a binder for biocomposites obtained from phosphogypsum was used and properties were compared to commercial gypsum. CO2 negative building material was developed with up to 92 kg CO2 ekv./m3 entrapped. Both physical and mechanical properties were studied. Thermal conductivity was in the range from 0.058 to 0.101 W/(m·K) and compressive strength from 0.10 to 0.57 MPa. Biodeterioration test results showed that the pH of 5.60–6.55 for biocomposites promoted a rapid mold growth. Mold growth was slightly lower for composites with higher gypsum content, while phosphogypsum induced more rapid mold growth. Cladosporium, Rhizopus, Chaetomium, and other molds were observed on samples even at an early stage of testing giving a serious concern about only limited application in dry conditions for such gypsum-based biocomposite. [ABSTRACT FROM AUTHOR]

In this study, novel blends of mycelium biocomposites (MB) were developed. Various combinations of birch sawdust and hemp shives with birch bark (BB) and wheat bran (WB) additives were inoculated with basidiomycete Trametes versicolor to produce self-growing biomaterials. MB were characterized according to mycelial biomass increment in final samples, changes in chemical composition, elemental (C, H, N) analyses, granulometry of substrates, water-related and mechanical properties, as well as mold resistance and biodegradability. The mycelial biomass in manufactured MB increased by ~100% and ~50% in hemp and sawdust substrates, respectively. The lignocellulose ingredients during fungal growth were degraded as follows: cellulose up to 7% and 28% in sawdust and hemp substrates, respectively, and lignin in the range of 13% in both substrates. A larger granulometric fraction in hemp MB ensured higher strength property but weakened water absorption (600–880%) performance. Perspective MB combinations regarding strength performance were hemp/BB and pure hemp MB (σ10 0.19–0.20 MPa; E 2.9 MPa), as well as sawdust/WB combination (σ10 0.23 MPa; E 2.9 MPa). WB positively affected fungal biomass yield, but elevated water absorption ability. WB improved compressive strength in the sawdust samples but decreased it in the hemp samples. BB supplement reduced water absorption by more than 100% and increased the density of sawdust and hemp samples. All MB samples were susceptible to mold contamination after full water immersion, with identified fungal genera Rhizopus, Trichoderma and Achremonium. The MB exhibited high biodegradability after 12 weeks' exposure in compost, and are therefore competitive with non-biodegradable synthetic foam materials. [ABSTRACT FROM AUTHOR]

The effect of thermo-hydro treatment (THT) on the durability of birch wood veneers and two plywood products - A and B was studied. THT was carried out in a multi-functional pilot scale wood modification device under elevated water vapour pressure at the following temperatures and treatment times (°C/min): 150/10; 150/50; 160/10 and 160/50. After THT, durability was examined according to the standards ENV 12038, EN 84 and EN 73. The durability class (DC) according to the CEN/TS 15083-1 was assigned to each treatment after fungal exposure. Water soluble extractives from THT samples were analysed with a UV-Vis Spectrometer in a range of 200 - 400nm, potentiometric titration and the Malaprade reaction. The best DC (2 - 3) for aged veneers was reached with the THT 160°C against the white rot fungus. The THT of industrial plywood A at 160°C enabled producing a material with improved durability (DC 1 - 3) after ageing. Plywood B with glued THT veneers showed lower decay resistance than plywood A. After ageing, better performance of plywood B was achieved at the THT 160°C assigning to DC 2 - 4. At the THT regimes under study, water soluble products (acids, sugars, aromatic compounds) were formed from both polysaccharides and lignin. [ABSTRACT FROM AUTHOR]

The white-rot fungus Trametes versicolor and brown-rot fungi Coniophora puteana, Gloeophyllum trabeum, and Postia placenta have a potential to produce a range of hydrolytic enzymes participating in the degradation of lignocellulosic materials. In the colonization of untreated and thermally modified wood blocks wheat bran-containing medium (WBA) greatly favored the secretion of xylanase by C. puteana and both endoglucanase and xylanase expression by T. versicolor, although rather higher carboxymethyl cellulase activity of C. puteana was observed on the malt extract-based medium (malt extract-containing agar (MEA). Coniophora puteana and T. versicolor are able to actively colonize and degrade the untreated pine and birch wood blocks. The mycelium applied on blocks in the form of pellets was more aggressive in wood degradation in comparison with the colonized mycelium. WBA favored the dry matter loss during the initial stages (10-20 days) of pine wood colonization; however, MEA further promoted more rapid wood degradation. The main finding was that the thermal treatment of birch wood was accompanied by a low degree of fungal colonization and increased durability against the fungal attack in spite of the high cellulase and xylanase activities. Thermal modification of wood was not toxic to the fungi because it did not disturb the secretion of the hydrolytic enzymes involved in wood degradation. The thermally modified hollocellulose is probably less susceptible to the enzymes involved in the degradation of polysaccharides or less accessible to hydrolytic enzymes due to the change of the cell wall structure. [ABSTRACT FROM AUTHOR]

Hot water extraction of pine wood was carried out after 10 days of exposure to brown-rot fungi ( Gloeophyllum trabeum, Coniophora puteana and Poria placenta) and a white-rot fungus ( Coriolus versicolor). The microstructure of the wood cell wall was analysed by the water vapour sorption method. The content and composition of monosaccharides in the extracts were determined by high performance liquid chromatography. Despite the absence of essential mass losses at the initial stage of the contact with the fungi, slight changes in the cell wall microstructure can be observed, namely, the hydrophobisation and the change in the character of porosity in the region of 1–10 nm pore sizes. After hot water treatment, the mass decreases considerably, i.e., to 25% for the control and brown-rot treated samples and more than 30% for the white-rot treated sample. The sorption-desorption isotherms for the washed control sample and brown-rot samples were not changed practically, but the isotherm was changed clearly in the case of the white-rot treated sample. The microstructure of the white-rot sample changed after hot water washing radically and was accompanied with the formation of wide mesopores in the range of 5–9 nm width. [ABSTRACT FROM AUTHOR]

In this study unique blended biopolymer mycocel from naturally derived biomass was developed. Softwood Kraft (KF) or hemp (HF) cellulose fibers were mixed with fungal fibers (FF) in different ratios and the obtained materials were characterized regarding microstructure, air permeability, mechanical properties, and virus filtration efficiency. The fibers from screened Basidiomycota fungi Ganoderma applanatum (Ga), Fomes fomentarius (Ff), Agaricus bisporus (Ab), and Trametes versicolor (Tv) were applicable for blending with cellulose fibers. Fungi with trimitic hyphal system (Ga, Ff) in combinations with KF formed a microporous membrane with increased air permeability (>8820 mL/min) and limited mechanical strength (tensile index 9–14 Nm/g). HF combination with trimitic fungal hyphae formed a dense fibrillary net with low air permeability (77–115 mL/min) and higher strength 31–36 Nm/g. The hyphal bundles of monomitic fibers of Tv mycelium and Ab stipes made a tight structure with KF with increased strength (26–43 Nm/g) and limited air permeability (14–1630 mL/min). The blends KF FF (Ga) and KF FF (Tv) revealed relatively high virus filtration capacity: the log10 virus titer reduction values (LRV) corresponded to 4.54 LRV and 2.12 LRV, respectively. Mycocel biopolymers are biodegradable and have potential to be used in water microfiltration, food packaging, and virus filtration membranes. [ABSTRACT FROM AUTHOR]

Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE). Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties. SEM and microscopy revealed the network structure of materials. The tensile index of materials was in the range of 8–60 Nm/g and air permeability ranged from 32–23,990 mL/min, depending on the composition of materials. HF was the key component for strength; however, the addition of FF to compositions resulted in higher air permeability. Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8–62% drop in the C/N ratio. Conclusions were drawn about the appropriateness of fungal hyphae for use in papermaking-like technologies together with cellulose fibers. Developed materials can be considered as an alternative to synthetic melt and spun-blown materials for PPE. [ABSTRACT FROM AUTHOR]

The demand for eco-friendly packaging materials has urged researchers to look for alternatives to petroleum-based polymers. In this regard, paper-based products have turned out to be a promising choice; however, their weak resistance to water has limited their application. The use of various additives to enhance paper's moisture resistance is a common practice. However, considering the growing global agenda for sustainable development, the search for new bio-based paper additives has become increasingly important. This study investigated the potential synergistic impact of the addition of nanofibrillated cellulose (NFC) and chitosan additives (CHIT) to different fiber combinations to improve paper's properties, in particular, their wet strength. The efficacy of the additive application order was examined and was found to be crucial in achieving the desired outcomes. The results showed that incorporating CHIT after NFC enhanced the paper's tensile and burst indicators, as well as the paper stretch in the dry state, by 35–70%, 35–55%, and 20–35%, respectively. In addition, the tensile index and stretch in the wet state improved 9–13 times and 2.5–5.5 times over, respectively. The air permeability decreased 2.5–12 times over. These findings demonstrate that the sequential addition of the NFC and CHIT additives yield a greater enhancement of paper's properties than using each additive separately. [ABSTRACT FROM AUTHOR]

Abstract: The study was focused mainly on the biodeterioration of the external wood of the Latvian Ethnographic Open-Air Museum, Riga (1924), and the sacral wooden buildings in Eastern Latvia (Latgale). The inspected wooden structures included windows, stairs, walls, roofs, fences, benches etc. Roofs, walls and fences were the most commonly decayed outdoor structures, while roof constructions and ceilings were the most often deteriorated indoor structures. Fungi from the phyla Basidiomycota, Ascomycota and Protozoa (Myxomycota) were identified. Common fungal genera were Antrodia, Gloeophyllum, Athelia, Hyphoderma, Hyphodontia, Pharenochaete, Postia and Botryobasidium. Mainly corticoid and poroid species were recorded in the wooden structures. The protection measures against the fungal attack should include a proper maintenance of roofs as well as the decrease/elimination of the vegetation around the buildings. The historical value of biodeteriorated structures should be taken into consideration before applying the protection/renovation measures. [Copyright &y& Elsevier]

The effect of thermo-hydro treatment (THT) on the properties of birch ( Betula spp.) wood veneers has been studied. THT was carried out in a multi-functional pilot scale wood modification device of wood treatment technology (WTT, Latvia) under elevated water vapor pressure conditions at four combinations of temperature and treatment time (°C/min): 150/10; 150/50; 160/10 and 160/50. After THT, the following veneer properties were examined: mass loss (ML), chemical composition, bending strength (BS), tensile strength (TS), equilibrium moisture content (EMC), resistance to decay by mould and blue stain fungi, and surface contact angle (CA). The chemical components were changed by THT. Increased THT temperature and time resulted in hydrophobization of veneers as indicated by decreasing EMC and increasing CA data. All THT were effective against wood discoloring fungi, although insufficient decay resistance was observed. The mechanical strength properties of THT veneers were also deteriorated. [ABSTRACT FROM AUTHOR]

Pinewood was modified by vacuum impregnation with various aqueous lignin solutions of low concentration (0.5- 1.0%) and its decay resistance was tested by the standard procedure EN 113. Five lignin types were tested against three brown rot and one white rot fungi. The bio-durability of wood was considerably increased by the treatment. The highest effect of modification was for alkali, kraft, hydrolysis lignins and industrial lignosulfonate, when mass losses of wood for brown-rot fungi were negligible. The effect of the modification with certified lignosulfonates was insignificant. Chemical analysis revealed that phenols, which are leached from lignin and are adsorbed by wood in the impregnation process, could act as a biocide. The hydrophilic properties of wood either did not change (certified lignins) or were enhanced because of some change in the pore structure (industrial lignins). Further tests are needed to verify the positive effects of this technologically simple and environmentally friendly treatment. [ABSTRACT FROM AUTHOR]

This study investigated the effect of phenol-formaldehyde (PF) resin treatment on the dimensional stability of birch solid wood and plywood. Therefore, three different low molecular weight PF resins with average molecular weights (Mw) of 292, 528, and 884 g/mol were synthesized and used for silver birch (Betula pendula) solid wood (20 × 20 × 20 mm3) and veneer (100 × 100 × 1.4 and 300 × 300 × 1.4 mm3) impregnation to produce plywood. The weight percent gain (WPG) and bulking after curing of resin treated wood specimens were determined. The leaching was performed to evaluate the PF resin fixation stability. All tested resins at all concentrations were similarly fixed in wood blocks after the leaching (1.5–2.0% WPG loss). The dimensional stability of birch wood after treatment with all tested PF resins was significantly improved. The anti-swelling efficiency (ASE) of birch wood blocks treated with PF resins after seven water soaking-drying cycles was in the range of 39–53%, 46–65% and 49–65% using 10, 15 and 20% solution concentrations, respectively. Whereas, the ASE of plywood obtained from veneers treated with 10% of PF solution was improved by 6–20%. The equilibrium moisture content (EMC) and volumetric swelling (VS) of PF treated plywood at 98% of relative humidity (RH) decreased significantly. All PF resin treated plywood surfaces were more hydrophobic compared to untreated plywood. [ABSTRACT FROM AUTHOR]

Thermal modification (TM) of wood has occupied a relatively narrow but stable niche as an alternative for chemical wood protection. There are different technological solutions for TM and not all details of their effects on wood tissue have been understood. The one-stage hydrothermal modification (HTM) at elevated vapour pressure essentially changes the wood's composition and structure. In the present paper, the changes in three hardwood lignins (alder, aspen, and birch) were observed within the cell wall by means of cellular UV microspectrophotometry. The lignin absorbances in the compound middle lamella (CML) of unmodified wood are 1.7- to 2.0- fold higher than those in the fibre S2 layer. The woods were modified in the temperature range from 140 to 180°C, while in the lower temperature range (140°C/1 h), the UV absorbances are little affected. Essential changes occur in the range of 160-180°C and the UV data reflect these by absorbtion changes, while the absorbances at 278 nm rise with factors around 2 more in the S2 layer than in the CML. The absorbance increments are interpreted as polycondensation reactions with furfural and other degradation products of hemicelluloses with the lignin moiety of the cell wall. [ABSTRACT FROM AUTHOR]

Micropropagated hybrid aspen clones (Populus tremuloides Michx.×Populus tremula L.) and a plus-tree (superior phenotypes selected) aspen (Populus tremula L.) were grown under similar conditions in the central part of Latvia. After cutting at the age of 12 years, 64 sample trees were examined. The dimensions of the vessels and the content of cellulose, lignin, extractives and ash were determined. The cell walls were characterised by UV microspectrophotometry (UMSP) in the UV range. The dimensions of fibres obtained by kraft pulping were determined such as length, width, and their shape factors. Coarseness measurements were performed, and strength properties of the handsheets were tested. One of the clones (no. 44) had a significantly higher stem volume of 208 dm³ and stem diameter of 17 cm at a height of 1.3 m. This clone had otherwise no notable differences to the other clones concerning the chemical composition, except a slightly higher lignin content (20.0% as compared with the mean value of 19.3% for other clones). Kraft cooking of hybrid aspen chips gave pulp yields in the range of 48.6-52.4%. Slightly higher strength properties (10-15%) were found for the handsheets prepared from a common aspen pulp (reference) as compared with those from hybrid aspen fibres. [ABSTRACT FROM AUTHOR]

In this study, the relationship between the properties of the wood and kraft pulp fibers as well as paper characteristics of 27-year-old trees, lodgepole pine (Pinus contorta) and Scots pine (Pinus sylvestris), was assessed. All trees had been grown in Latvia, within the same forest type, Myrtillosa. Wood density, year ring width, chemical composition and cross-sectional cell wall dimensions were measured. Fiber characteristics were determined, and handsheets were made for all samples from unbeaten kraft pulp. The results showed that the amount of latewood had a positive correlation with wood density for both species and with further positive impact on the paper burst index. Also, slight differences in cross-sectional dimensions were observed. Lodgepole pine provided paper with higher burst strength than Scots pine. Since the former is of higher density, less wood per volume is needed to produce a ton of pulp, and results showed a higher pulp yield in the case of lodgepole pine. [ABSTRACT FROM AUTHOR]