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Gentamicin is a broad spectrum antibiotic employed for the cure of infections caused by both Gram-positive and Gram-negative bacteria. It is eliminated through urine in the unmetabolized and microbiologically active form, leading to the emergence of gentamicin-resistant bacteriocoenosis. Green synthesis of methyl gallate conjugated silver nanoparticles (MG–AgNPs) is reported in this study. The synthesized MG–AgNPs were found to be stable at high temperature, in the pH range 4 to 8, and with up to 100 mM NaCl concentration in the solution. Further, the synthesized MG–AgNPs were employed as a selective and sensitive probe for the detection of gentamicin. The proposed colorimetric aggregation sensor significantly detected gentamicin over a wide range of concentrations in the presence of other interfering drugs and metal ions. UV-visible spectroscopy, FTIR, and atomic force microscopy were employed to confirm the sensing phenomenon. The binding ratio of MG–AgNPs with gentamicin was found to be equimolar. The limit of detection and quantification were found to be 0.29 μM and 1 μM, respectively, in the concentration range of 1–100 μM. The proposed sensor significantly detects gentamicin in environmental waters, human blood plasma, serum, and urine.

We report here the synthesis as well as antioxidant activity of a series of 2-aryl thiazolidine-4-carboxylic acids, including two novel derivatives. They were synthesized by nucleophilic cyclic condensation of L-cysteine hydrochloride with a range of aromatic aldehydes. Their in vitro antioxidant activity was evaluated by DPPH radical scavenging assay. It was observed that the aromatic substituent at C-2 of thiazolidine ring effects the antioxidant potential of the thiazolidine derivatives. The nature and position of the substituents on aromatic ring were correlated with antioxidant activity. Compounds with -OCH

Silver nanoparticles (SN) have been recently developed as a new class of antimicrobial agents against numerous pathogenic microorganisms. SN have also been used as efficient drug delivery systems and have been linked with increasing drug potency. Here, we demonstrated the enhanced antifungal efficacy of nystatin (NYT) and fluconazole (FLU) after conjugation with SN. The antifungal bioactivity of NYT- and FLU-coated SN was evaluated against Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404 by the agar tube dilution method. The aim of this study was to determine and compare the antifungal efficacy of NYT and FLU with their SN and, finally, the combination of both nanoparticles as NYT-SN + FLU-SN against pathogenic fungi. The results indicated that all test samples showed a dose-dependent response against tested fungi. SN significantly enhanced the antifungal effects of NYT and FLU as compared to drugs alone. We observed a remarkable increase in the percent inhibition of both fungi (90-100%) when treated with a combination of both nanoparticles NYT-SN + FLU-SN at 200 μg/mL only. Furthermore, the morphological modifications occurred at the surface of fungal species were also analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). While tested against primary human cell line, all SN showed negligible cytotoxicity. Hence, these results suggest that the combination of SN with NYT and FLU may have clinical implications in the treatment of fungal infections. However, in vivo studies are needed before recommending the use of these nanoparticles safely in clinical situations.

A different approach to the molecular recognition events consists in synthesis and conjugating silver nanoparticles with flavonoid quercetagetin isolated from T. erecta. In this article we are reporting rapid and feasible procedure for the synthesis of querecetagetin stabilized silver nanoparticle (Qt AgNPs). These nanoparticles were characterized by UV–vis spectroscopy, FTIR, and AFM techniques. The average size of the querecetagetin coated silver nanoparticles was found to be in the range from 25 to 45 nm and are highly robust. Querecetagetin coated silver nanoparticle selectively recognize amoxicillin producing a bathochromic shift along with color change from yellow to reddish. The detection limit was calculated using standard deviation of blank and slope of regression line and was found to be 4.46 μM with a correlation factor R2 equal to 0.993. The binding stoichiometry between Qt AgNPs and amoxicillin was found to be 1:2 (Qt AgNPs: amoxicillin) as revealed by Job‘s plot. The Qt AgNPs interact selectively with amoxicillin in human blood plasma and tap water sample showing that the ingredients of tap water and blood plasma are inert towards the interaction of Qt AgNPs with amoxicillin.Photophysical events were examined using UV–vis, FTIR spectroscopy and, AFM techniques.

A new click generated triazole based chemosensor 5 was designed and synthesized, that exhibits excellent selectivity for Fe +3 ion over a wide range of tested metal ions e.g. NH 4 + , Li + , Na + , K + , Cu + , Ag + , Ca +2 , Ni +2 , Co +2 , Cd +2 , Mg +2 , Cu +2 , Zn +2 , Pb +2 , Hg +2 , In +3 , La +3 and Y +3 in an aqueous medium without any remarkable interference. With Fe +3 , the sensor 5 showed enhancement in absorption intensity at wavelength of 262 nm. The sensor forms a host-guest complex in 1:1 stoichiometry with the detection limit down to 3.21 μM. The interaction was also confirmed by FT-IR, 1 H NMR and FAB showing the Sensor 5 –Fe +3 adduct at 495. Further, the sensor was successfully utilized to detect Fe +3 in human blood plasma and laboratory tap water as well.

Synthesis, characterization and molecular recognition properties of fluorene based supramolecular cleft 1 is reported. The cleft molecule 1 was prepared in a single-step with good yield (85% yield), by linking Fluorene with 1-ethyl piperazine. The cleft molecule 1 was carefully characterized using various spectroscopic techniques such as NMR and mass spectrometry. The supramolecular interaction of cleft 1 with amoxicillin, 6APA, aspirin, captopril, cefotaxime, ceftriaxone, cefuroxime, diclofenac, penicillin, and cephradine was evaluated by fluorescent spectroscopy. The molecular recognition studies showed that amoxicillin selectively binds with cleft 1 in the presence of other drugs. The analytical method developed for the supramolecular interaction of molecular cleft 1 and amoxicillin was validated at varying pH, concentration and temperature during recognition process. Job's plots indicated that the stochiometry of the interactions between the cleft 1 and the amoxicillin was 1:1.

Three biologically active new Schiff bases, 2-[(3-hydroxybenzylidene)amino]phenol 5 , 2-[(4-hydroxybenzylidene)amino]phenol 6 and 4-[(2-hydroxyphenylimino)methyl]benzene-1,3-diol 7 , were synthesized by the reaction of 2-aminophenol 1 with three different hydroxyl-benzaldehydes 2 – 4 . They were characterized by spectroscopic analysis (IR, 1 H NMR, EI-MS) along with elemental analyses. The products were biological screened out for antioxidant, lipoxygenase inhibition, antibacterial and urease inhibition activities. The compounds 5 and 6 showed potent while 7 showed moderate antioxidant activity. Compound 6 showed potent whereas 5 and 7 showed significant lipoxygenase inhibition activity. All the target compounds showed excellent activities against Staphylococcus intermedius , Bacillus subtilis , Staphylococcus aureus, Escherichia coli and Salmonella typhi bacteria. All the compounds showed non-significant activity against urease enzyme.

Antioxidant and anticholinesterase activities of twelve flavonoids i.e. santin ( 1 ), penduletin ( 2 ), viscosine ( 3 ), 6,7-dimethylkaempferol ( 4 ), kaempferol-​3-​methylether ( 5 ), 3,4′-dimethoxy-5,7-dihydroxyflavone ( 6 ), 5,7,4′-trihydroxy-3′-(3-hydroxymethylbutyl)-3,6-dimethoxyflavone ( 7 ), 5,7-dihydroxy-3′-(2-hydroxy-3-methyl-3-butenyl)-3,6,4′-trimethoxy-flavone ( 8 ), 5,7-dihydroxy-3′-(3-hydroxy-methylbutyl)-3,6,4′-trimethoxyflavone ( 9 ), 5,7-dihydroxy-3′-(4′′-acetoxy-3′′-methylbutyl)-3,6,4′-trimethoxy flavone ( 10 ), aromadendrin, (2S,3S) 3,4′,5,7-tetrahydroxyflavanone ( 11 ), and pinocembrin ( 12 ), together with monohydrated crystal ( 1A ) of 1 and anhydrous crystal ( 3A ) of 3 – isolated from Dodonaea viscosa – were studied. The results are given as 50% inhibition concentrations (IC 50 ). Their structure–activity relationships are discussed for both activities. Flavonoids 3 and 10 showed higher antioxidant activity in all tests. 3 Also showed moderate acetylcholinesterase (IC 50 : 182.97 ± 1.25 μM) and significant butyrylcholinesterase (IC 50 : 47.07 ± 0.54 μM) inhibitory activities. Compounds 2 (IC 50 : 55.78 ± 1.01 μM) and 3A (IC 50 : 58.72 ± 1.02 μM) were also moderate anticholinesterase agents against butyrylcholinesterase enzyme. Monohydrate crystal ( 1A ) showed better antioxidant and anticholinesterase activities than its amorphous form ( 1) . In contrast, the amorphous 3 exhibited better activity than the anhydrous crystal ( 3A ). In hydrous crystal, the water molecule might be responsible for better activity. These natural flavonoids can be used as pharmaceuticals and/or preservatives in respective industries.

Six Schiff bases 8–13 were synthesized by condensation of 2-aminophenol (1) with various chloro- and nitro-benzaldehydes (2–7), characterized, and evaluated for antioxidant, antibacterial, lipoxygenase, and urease inhibitory potentials. All Schiff bases except 11 showed potent antioxidant activity with IC50 values ranging between 17.2 and 33.1 μM when compared with standard BHA (IC50 = 44.2 μM); especially, compound 10 (IC50 = 17.2 μM) and compound 13 (IC50 = 18.6 μM) exhibited much better potential than the standard BHA. In lipoxygenase inhibition activity, all products except 8 showed excellent-to-good potential with IC50 values ranging from 22.5 to 110.5 μM while comparing with standard baicalein (IC50 = 22.6 μM). Compound 10 (IC50 = 22.5 μM) showed lipoxygenase potential comparable to the standard baicalein (IC50 = 22.6 μM), and molecular docking simulations showed that nitro group of 10 penetrated deeply into a pocket surrounded by basic amino acid residues which revealed the excellent activity of 10. All products also showed good potential against all gram-positive S. intermedius, B. subtilis, and S. aureus, and moderate activity against gram-negative E. coli and S. typhi bacteria. However, the products showed nonsignificant inhibitory potential against urease enzyme.

A ligand L has been synthesized by the condensation of 4-chlorobenzaldehyde and 2-amino-5-chlorobenzophenone. The complexes 1–5 of Pb+2, Ni+2, Co+2, Cu+2 and Cd+2 with ligand were prepared. The newly synthesized ligand L and its complexes 1–5 were characterized on the basis of elemental analyses, molar conductance, 1H NMR, IR and MS spectral data. The ligand was also characterized by X-ray crystallography. By electronic spectra and magnetic moment data, the square planar geometry for complex 1 and the octahedral geometry for complexes 2–5 were proposed. The conductivity data suggests non-electrolytic nature of the complexes 1–5. The complexes have higher antibacterial activity than the parent ligand L.

The bioassay-guided fractionation of Daphne retusa Hemsl. has led to the isolation of a new aryl tetrahydronaphthalene lignan derivative named as daphnretusic acid (1), along with six new source compounds such as 5,7-dihydroxyflavone (2), 7-hydroxyflavone (3), 6-methoxyflavone (4), (+) pinoresinol (5), (+) sesamin (6), and β-sitosterol-3-O-β-D-glucopyranoside (7). Their structures were elucidated by (1)H NMR, (13)C NMR, 1D, 2D NMR, UV, IR, and EIMS analyses. All the fractions (n-hexane, CHCl3, AcOEt, CH3OH, and water) and pure compounds (1-7) were subjected to the assay of urease and α-chymotrypsin inhibitory activities. Chloroform and methanol soluble fractions showed moderate urease inhibition. Compound 2 exhibited significant urease inhibition with IC50 value 60.4 ± 0.72 μM, whereas compounds 1 and 3-7 remained inactive during urease inhibition and α-chymotrypsin bioassays.

Schiff bases, 2-{[(2-hydroxyphenyl)methylidene]amino}phenol (4) and 2-{[3-4-(dimethylamino)phenyl-2-propenylidene]amino}phenol (5), have been synthesized by the condensation of 2-aminophenol (1) with 2-hydroxybenzaldehyde (2) and 4-dimethylaminocinnamaldehyde (3), respectively. They showed good lipoxygenase inhibition and antioxidant activities, as compound 4 showed potent antioxidant activity. The products also showed good activities against Gram-positive: S. intermedius, B. subtilis and S. aureus, and Gram-negative: E. coli and S. typhi bacteria, as compounds 5 and 4 are excellent active against B. subtilis and S. typhi, respectively. These good to potent activities may be due to the presence of free hydroxyl and amino groups. They showed non-significant urease inhibition activity.

New dimeric and a trimeric coumarin glucosides namely Daphneretusin A (1) Daphneretusin B (2) along with three known oligomers (3-5) were obtained as a result of bioassay guided fractionation of Daphne retusa Hemsl. Fractions (n-hexane, CHCl3, AcOEt, CH3OH and water) exhibited potent radical scavenging activity in relevant non-physiological bioassays. The structures of isolated compounds were elucidated by UV, IR, EIMS, FAB-MS, 1D, and 2D NMR spectroscopic analysis.

WOS: 000382572400017 PubMed ID: 26866457 ContextDodonaea viscosa (L.) Jacq (Sapindaceae) has been used in traditional medicine as antimalarial, antidiabetic and antibacterial agent, but further investigations are needed.Objective This study determines the antioxidant and anticholinesterase activities of six compounds (1-6) and two crystals (1A and 3A) isolated from D. viscosa, and discusses their structure-activity relationships.Materials and methods Antioxidant activity was evaluated using six complementary tests, i.e., -carotene-linoleic acid; DPPH center dot, ABTS(center dot+), superoxide scavenging, CUPRAC and metal chelating assays. Anticholinesterase activity was performed using the Elman method.Results Clerodane diterpenoids (1 and 2) and phenolics (3-6) - together with three crystals (1A, 3A and 7A) - were isolated from the aerial parts of D. viscosa. Compound 3A exhibited good antioxidant activity in DPPH (IC50: 27.441.06M), superoxide (28.18 +/- 1.35% inhibition at 100M) and CUPRAC (A(0.5): 35.89 +/- 0.09M) assays. Compound 5 (IC50: 11.02 +/- 0.02M) indicated best activity in ABTS assay, and 6 (IC50: 14.30 +/- 0.18M) in -carotene-linoleic acid assay. Compounds 1 and 3 were also obtained in the crystal (1A and 3A) form. Both crystals showed antioxidant activity. Furthermore, crystal 3A was more active than 3 in all activity tests. Phenol 6 possessed moderate anticholinesterase activity against acetylcholinesterase and butyrylcholinesterase enzymes (IC50 values: 158.14 +/- 1.65 and 111.60 +/- 1.28M, respectively).Discussion and conclusion This is the first report on antioxidant and anticholinesterase activities of compounds 1, 2, 5, 6, 1A and 3A, and characterisation of 7A using XRD. Furthermore, the structure-activity relationships are also discussed in detail for the first time. Scientific and Technological Research Council of Turkey (TUBITAK-BIDEB)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2216, 2211] Authors are thankful to The Scientific and Technological Research Council of Turkey (TUBITAK-BIDEB 2216 and 2211 programs) to provide financial support.

Two new xanthonolignoids, hypericorin A (1) and hypericorin B (2), along with five known new source compounds, a xanthonolignoid, kielcorin (3), 4-hydroxy-2,3-dimethoxyxanthone (4), 3,4,5-trihydroxyxanthone (5), 1,3-dihydroxy-5-methoxyxanthone ( 6) and 1,3,7-trihydroxyxanthone (7), were isolated from the stems (twigs) of Hypericum oblongifolium Wall. The structures of the new compounds were deduced on the basis of spectroscopic techniques (EI-MS, HREI-MS, (1)H NMR, (13)C NMR, HMQC, HMBC, and NOESY). We also report herein for the first time the single crystal X-ray structure of compound 6. Compounds 1- 7 were screened for their IN VITRO anti-inflammatory (respiratory burst) inhibiting activities using isolated human neutrophils; compounds 1, 2, 3, 5, and 7 showed significant activities (IC (50) = 816.23 ± 73.30, 985.20 ± 55.80, 965.21 ± 65.80, 907.20 ± 50.80, 975.20 ± 81.10 µM, respectively), compound 6 showed moderate activity (IC (50) = 2500.85 ± 50.50 µM), while compound 4 was totally inactive at 1000 µg/mL as compared to the positive control used, indomethacin (IC (50) = 757.99 ± 5.90 µM), and aspirin (IC (50) = 279.44 ± 4.40 µM). Compound 4 was also inactive in comparison with other tested Hypericum compounds.

Two new coumarins, murrmeranzin (1) and murralonginal (2), together with four known compounds minumicrolin (3), murrangatin (4), meranzin hydrate (5) and hainanmurpanin (6) have been isolated from the aerial parts of Murraya paniculata. The structures of these compounds were determined through spectral analysis. Minumicrolin (3) showed mild butyrylcholinesterase inhibition activity.

Two new oxindole alkaloids isatinone A (1) and B (2) have been isolated from Isatis costata, along with the known trisindoline. Their structures have been assigned on the basis of spectroscopic techniques and chemical studies. Both new compounds showed significant antifungal activity.

A novel fluorescent bis-calix[4]arene macrocycle 9 incorporating metal-binding pockets was successfully prepared. The structure of macrocycle 9 and its precursors were characterized via EI-MS, MALDI-TOF-MS, ESI-MS, (1)H NMR, (13)CNMR, 2D NMR, and X-ray crystallography. The macrocycle 9 displayed selective fluorescence quenching after interacting with Cu(2+) in the presence competing metal cations including Mg(2+), Ca(2+), Ba(2+), Ag(+), Zn(2+), Ti(4+),Cd(2+), Hg(2+), Pb(2+), In(3+), La(3+), Cr(3+), Ni(2+), Sb(3+), V(5+), Fe(3+), Co(2+), Sn(2+), Sn(2+), and Tl(+). The Cu(2+) limit of detection was found to be 40 nM much lower than its threshold level (∼ 20 μM) in drinking water permitted by the U.S Environmental Protection Agency (EPA). Furthermore, drinking water samples from Karachi University (Pakistan) spiked with Cu(2+) were analysed with the sensing system and the results showed an excellent agreement with the fluorescence quenching phenomenon of macrocycle 9 examined in deionized water. Importantly, the chemosensor 9 could be used to detect Cu(2+) in living cells.

The objective of the present study was to evaluate the modulation of GABA-evoked currents by the flavonoid viscosine at recombinant GABA(A) receptors, and subsequently to study its anxiolytic, sedative and anticonvulsant activities. Viscosine (1-300μM) positively modulated GABA-evoked currents at human α1β2γ2L and α2β2γ2L GABA(A) receptors expressed in Xenopus oocytes in a flumazenil insensitive manner. In behavioral studies, viscosine at doses of 10-100mg/kg (i.p.) exerted significant anxiolytic effects in the elevated plus maze, light-dark and open field tests (*P0.05, **P0.01, ***P0.001 n=6, One-way ANOVA post-Dunnett's test), and sedative effects at high doses (100mg/kg i.p.) in hole board and thiopental induced sleep time tests. The anxiolytic effect in the elevated plus maze test was not blocked by flumazenil whereas pentylenetetrazole (PTZ) completely attenuated the effect, indicating that the activity was mediated via the non-benzodiazepine sites of GABA(A) receptors. Furthermore, viscosine at doses of 10-100mg/kg (i.p.) exerted anticonvulsant effects in a dose-dependent manner in PTZ, picrotoxin and bicuculline induced seizure paradigms (*P0.05, **P0.01,***P0.001 n=6, One-way ANOVA post-Dunnett's test). In conclusion, the results of the present study suggest that the anxiolytic and anticonvulsant actions of viscosine are likely mediated via its positive allosteric modulatory action of GABA at different GABA(A) receptor subtypes.

A new spirostane steroidal saponin, govanoside A (1) along with three known compounds borassoside E (2) pennogenin (3) and diosgenin (4) were isolated from rhizomes of Trillium govanianum. Their structures were elucidated through 1D, 2D-NMR spectroscopic data analysis and acid hydrolysis. Compound (2) in genus Trillium and all compounds (1-4) in T. govanianum are reported herein for the first time. Furthermore, compounds 1 &2 exhibited good to moderate activities against Aspergillus niger ATCC 16888, Aspergillus flavus ATCC 9643, Candida albicans ATCC 18804, and Candida glabrata ATCC 90030. This is a significant finding keeping in view the limited antifungal drugs for aspergillosis and candidiasis.

Schiff bases, having azomethine group and their metal complexes are widely used for industrial purposes and also reveal a wide range of biological applications. This review describes the most promising biological activities of Schiff bases and their metal complexes. A general idea for synthetic methodologies used for the synthesis of Schiff bases and their metal complexes is also discussed.

Steroidal galactoside 1 and aryl esters 2 and 3 have been isolated from Buddleja crispa, along with ginipin 4, gardiol 5, 1-heptacosanol 6, and methyl benzoate 7, isolated for the first time from this species. The structures of all of the compounds were determined by spectroscopic techniques and chemical studies. The steroidal galactoside 1 is an inhibitor of lipoxygenase. Compounds 1- 3 displayed inhibitory activity against butyrylcholinesterse, while compounds 2 and 3 further showed inhibition against acetylcholinesterase

Two new lignans trivially named negundins A (1) and B (2), were isolated along with (+)-diasyringaresinol (3), (+)-lyoniresinol (4), vitrofolal E (5) and vitrofolal F (6), reported for the first time from this species. The structures of the new compounds were established through spectral studies. Compound 2 showed potent inhibitory activity against lipoxygenase enzyme, while 5 showed moderate activity against butyryl-cholinesterase.

Three C-alkylated flavonoids 7-O-alpha-D-glucopyranosyl-3,5-dihydroxy-3'-(4"-acetoxyl-3"-methylbutyl)-6,4'-dimethoxyflavone (1), 7-O-alpha-D-glucopyranosyl-3,4'-dihydroxy-3'-(4"-acetoxyl-3"-methylbutyl)-5,6-dimethoxyflavone (2), 3,7,4'-trihydroxy-3'-(8"-acetoxy-7"-methyloctyl)-5,6-dimethoxyflavone (3) and a trans-clerodane type diterpenoid (-)-6beta-hydroxy-5beta,8beta,9beta,10alpha-cleroda-3,13-dien-16,15-olid-18-oic acid (4) are reported from Duranta repens along with (+)-hardwickiic acid (5) and (+)-3,13-clerodadien-16,15-olid-18-oic acid (6), isolated for the first time from this species. Their structures were established on the basis of the spectral methods, especially two dimensional (2D) NMR spectroscopy.

Emodinol, a new oleane type triterpene, has been isolated from the chloroform soluble fraction of Paeonia emodi. The structure 1beta, 3beta, 23-trihydroxyolean-12-en-28-oic acid 1 has been assigned on the basis of spectral studies including 2D NMR. It showed significant beta-glucuronidase inhibitory activity. In addition benzoic acid and 3-hydroxybenzoic acid have also been reported for the first time from this species.

A new methylene-bridged bisflavonoid, methylenebissantin (1), and nine known compounds, including flavonoids (2–5), diterpenoids (6 and 7), and phenol derivatives (8–10) were isolated from the aerial parts of Dodonaea viscosa Jacq. The structure elucidation was based on spectroscopic data analyses. The isolated compounds were evaluated for the inhibition of Plasmodium falciparum enoyl-ACP reductase (PfENR). Methylenebissantin (1) exhibited a moderate inhibition (IC50 91.13 μM) against PfENR.

Isoprenylated flavonoids 5,7-dihydroxy-3'-(2-hydroxy-3-methyl-3-butenyl)-3,6,4'-trimethoxyflavone (1), 3,7-dihydroxy-3'-(2-hydroxy-3-methyl-3-butenyl)-5,6,4'-trimethoxyflavone (2) and an isoprenylated acetophenone derivative (3) have been isolated from Duranta repens along with known compounds, 5-hydroxy-3,6,7,4'-tetramethoxyflavone (4), rosenonolactone (5), 6,7-dimethoxycoumarin (6), 5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol (7) and 5alpha,8alpha-epidioxyergosta-6,9(11),22-trien-3beta-ol (8), isolated for the first time from this species. Their structures and the relative configuration were determined by spectroscopic methods (1H- and 13C-NMR, IR, UV and MS) and two-dimensional (2D)-NMR experiments. The compounds 1-5 showed inhibitory activity against prolyl endopeptidase while 4 and 5 were also active against thrombin.

Two new oleanane triterpenes; 2alpha,3alpha,24-trihydroxyolean-12-ene-28,30-dioic acid ([structure: see text]) and 2alpha,3alpha,24,28-tetrahydroxyolean-12-ene ([structure: see text]) have been isolated from the roots of Atropa acuminata. Anti-oxidant p-hydroxyphenethyl trans-ferulate ([structure: see text]), beta-sitosterol-3-O-beta-D-glucopyranoside ([structure: see text]) and oleanolic acid ([structure: see text]) have also been reported for the first time from this species. The structures were determined by spectroscopic studies including 2D-NMR.