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An acute animal experiment was performed to observe factors influencing the functional status of the aortic valve functional status after continuous-flow left ventricular assist device (CF-LVAD) implantation in an ovine model, and a physiologic predictive model was established.A CF-LVAD model was established in Small Tail Han sheep. The initial heart rate (HR) was set to 60 beats/min, and grouping was performed at an interval of 20 beats/min. In all groups, the pump speed was started from 2000 rpm and was gradually increased by 50-100 rpm. A multi-channel physiological recorder recorded the HR, aortic pressure, central venous pressure, and left ventricular systolic pressure (LVSP). A double-channel ultrasonic flowmeter was used to obtain real-time artificial vascular blood flow (ABF). A color Doppler ultrasound device was applied to assess the aortic valve functional status. Multivariate dichotomous logistic regression was used to screen significant variables for predicting the functional status of the aortic valve.Observational studies showed that ABF and the risk of aortic valve closure (AVC) were positively correlated with pump speed at the same HR. Meanwhile, the mean arterial pressure (MAP) was unaltered or slightly increased with increased pump speed. When the pump speed was constant, an increase in HR was associated with a decrease in the size of the aortic valve opening. This phenomenon was accompanied by an initial transient increase in the ABF and MAP, which subsequently decreased. Statistical analysis showed that the AVC was associated with increased pump speed (OR = 1.02, 95% CI = 1.01-1.04, p = 0.001), decreased LVSP (OR = 0.95, 95% CI = 0.91-0.98, p = 0.003), and decreased pulse pressure (OR = 0.82, 95% CI = 0.68-0.96, p = 0.026). ABF or MAP was negatively associated with the risk of AVC (OR 1). The prediction model of AVC after CF-LVAD implantation exhibited good differentiation (AUC = 0.973, 95% CI = 0.978-0.995) and calibration performance (Hosmer-Lemeshow χThe pump speed, LVSP, ABF, MAP, and pulse pressure are significant predictors of the risk of AVC. Predictive models built from these predictors yielded good performance in differentiating aortic valve opening and closure after CF-LVAD implantation.

OBJECTIVE: Developing and assessing a risk prediction model of postoperative atrial fibrillation (POAF) after coronary artery bypass grafting (CABG), and aims to provide a reference for the prediction and prevention. DESIGN: A retrospective case-control study. SETTING: Three major urban teaching and university hospitals and tertiary referral centers. PARTICIPANTS: consecutive patients undergoing CABG. INTERVENTIONS: The study was retrospective and no interventions were administered to patients. MEASUREMENTS AND MAIN RESULTS: In the study, the overall new-onset POAF prevalence was approximately 28%. A prediction model for POAF with nine significant indicators was developed, and identified new predictors of POAF: left ventricular end diastolic diameter (LVEDD), intraoperative defibrillation, and intraoperative temporary pacing lead implantation. The model had good discrimination in both the derivation and validation cohorts, with the area under the receiver operating characteristic curves (AUCs) of 0.621 (95% CI = 0.602–0.640) and 0.616 (95% CI = 0.579–0.651), respectively, and showed good calibration. Compared with CHA(2)DS(2)-VASc, HATCH score, and the prediction model of POAF after CABG developed based on a small sample of clinical data from a single center in China, the model in this study had better discrimination. CONCLUSION: We have developed and validated a new prediction model of POAF after CABG using multicenter data that can be used in the clinic for early identification of high-risk patients of POAF, and to help effectively prevent POAF in postoperative patients.

Network analysis has contributed to studies of the interactions of microorganisms and the identification of key populations. However, such analysis has rarely been conducted in the study of reservoir bacterioplankton communities. This study investigated the bacterioplankton community composition in the surface water of the Danjiangkou Reservoir using the Illumina MiSeq sequencing platform. We observed that the bacterioplankton community primarily consisted of 27 phyla and 336 genera, including Actinobacteria, Proteobacteria, and Bacteroidetes, demonstrating the richness of the community composition. Redundancy analysis of the bacterioplankton communities and environmental variables showed that the total nitrogen (TN), pH, chemical oxygen demand (COD), and permanganate index (CODMn) were important factors affecting the bacterioplankton distribution. Network analysis was performed using the relative abundances of bacterioplankton based on the phylogenetic molecular ecological network (pMEN) method. The connectivity of node i within modules (Zi), the connectivity of node i among modules (Pi), and the number of key bacteria were high at the Taizishan and Heijizui sites, which were associated with higher TN contents than at the other sites. Among the physicochemical properties of water, TN, ammonia nitrogen (NH4-N), pH, COD, and dissolved oxygen (DO) might have great influences on the functional units of the bacterial communities in bacterioplankton molecular networks. This study improves the understanding of the structure and function of bacterioplankton communities in the Danjiangkou Reservoir.

The aim of the present study was to investigate the PAQR3 gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level of PAQR3 gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P

Planktonic fungi are important components of aquatic ecosystems, and analyses of their community composition and function have far-reaching significance for the ecological management and maintenance of reservoir environments. However, few studies have investigated the composition, distribution, and function of planktonic fungi in reservoir ecosystems and their relationship with water quality. Here, the composition of the planktonic fungal community in the surface water layer of the Danjiangkou Reservoir is investigated using Illumina MiSeq sequencing. According to the results, the reservoir community is primarily composed of 7 phyla, including Ascomycota, Rozellomycota, Basidiomycota, Chytridiomycota, and Zygomycota, comprising 294 genera, demonstrating the rich diversity of this community. Redundancy analysis (RDA) of the planktonic fungal community and environmental factors showed dissolved oxygen (DO), chemical oxygen demand (COD), total nitrogen (TN), chlorophyll a (Chl a), and permanganate (CODMn) to be important factors influencing the distribution of planktonic fungi. Spearman correlation analysis of the planktonic fungal community composition and diversity indices with physical and chemical water quality parameters showed that the impacts of TN, COD and DO were the most significant. The results of this study on the planktonic fungal community in the Danjiangkou Reservoir area using high-throughput sequencing revealed that the community is sensitive to water quality parameters. This result provides a reference for studying the composition and distribution of the planktonic fungal community in Danjiangkou Reservoir and its role in the biogeochemical cycle.

AIM: To determine the expression and significance of filamin A (FLNa) in colorectal adenocarcinoma tissue. METHODS: The expression of FLNa in 46 colorectal cancer tissues and normal tissues was detected by immunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, and its relationship with clinical parameters and prognosis was analyzed. RESULTS: The positive expression of FLNa in cancer tissues was lower than that in normal mucosa, and the difference was statistically significant. The expression of FLNa correlated with liver metastasis, lymph node metastasis and rectal invasion depth, regardless of sex, age, tumor location, tumor size, gross shape and histological type of colorectal carcinoma. Multivariate analysis showed that FLNa was an independent risk factor for postoperative survival of patients with colorectal adenocarcinoma. Moreover, survival analysis showed that the expression level of FLNa was closely related with survival of patients with colorectal adenocarcinoma. The results of RT-PCR and Western blotting were consistent with those of immunohistochemistry. CONCLUSION: FLNa showed low expression in colorectal adenocarcinoma, high correlation with the incidence and development of colorectal cancer, and was considered an indicator of prognosis.

Tubular hydropower station is a better way in the development of low-head hydraulic power resources. More and more people begin to study the tubular turbine in depth with the development of hydraulic power recourses in the direction of low head and large capacity. With the unique structural features and adaptive head range of tubular turbine, its development is very fast in recent years. The strength analysis of a tubular turbine is an important research aspect with the increasing of its capacity. Bulb turbine is one of the main types of tubular turbines. This paper uses CATIA software and finite element analysis to analyze and calculate the strength and deformation of key components of a bulb turbine, so as to ensure the bulb turbine's safe and reliable operation.

The complete chloroplast (cp) genome of Caragana microphylla (Fabaceae) has been characterized from Illumina pair-end sequencing. The whole cp genome is relatively short (130,029 bp) because it lacks an inverted repeat (IR) region. It comprises 110 genes, including four unique rRNAs, 30 tRNAs, and 76 protein-coding genes. Similar to other closely related plastomes, rpl22 and rps16 are absent. The overall GC content of C. microphylla cp genome is 34.3 %. The phylogenetic analysis suggested that C. microphylla is closely related to the genera of Astragalus.

Scrophularia ningpoensis has exhibited a variety of biological activities and been used as a pharmaceutical product for the treatment of inflammatory ailment, rheumatoid arthritis, osteoarthritis and so on. Harpagoside (HAR) is considerer as a main bioactive compound in this plant. Serum albumin has important physiological roles in transportation, distribution and metabolism of many endogenous and exogenous substances in body. It is of great significance to study the interaction mechanism between HAR and bovine serum albumin (BSA). The mechanism of interaction between HAR and BSA was investigated using 2D and 3D fluorescence, synchronous florescence, ultraviolet spectroscopy and molecular docking. According to the analysis of fluorescence spectra, HAR could strongly quench the fluorescence of BSA, and the static quenching process indicated that the decrease in the quenching constant was observed with the increase in temperature. The magnitude of binding constants (KA) was more than 1×10⁵ L·mol⁻¹, and the number of binding sites(n) was approximate to 1. The thermodynamic parameters were calculated through analysis of fluorescence data with Stern-Volmer and Van't Hoff equation. The calculated enthalpy change (ΔH) and entropy change (ΔS) implied that the main interaction forces of HAR with BSA were the bonding interaction between van der Waals forces and hydrogen. The negative values of energy (ΔG) demonstrated that the binding of HAR with BSA was a spontaneous and exothermic process. The binding distance(r) between HAR and BSA was calculated to be about 2.80 nm based on the theory of Frster's non-radiation energy transfer, which indicated that energy is likely to be transfer from BSA to HAR. Both synchronous and 3D florescence spectroscopy clearly revealed that the microenvironment and conformation of BSA changed during the binding interaction between HAR and BSA. The molecular docking analysis revealed HAR is more inclined to BSA and human serum albumin (HSA) in subdomain ⅡA (Sudlow's site I). This study will provide valuable information for understanding the action mechanism of HAR.

In view of the multi-satellite formation establishment, the fuel consumption was optimized based on the Niche Genetic Algorithm. Some conclusions were drawn.

Aim to the path programme problem of satellite formation reconfiguration under low thrust, optimization is simulated based on Gauss Pseudospectral Method (GPM). The simulation result demonstrates that the GPM can effectively solve the the path optimization problem.

In view of fuel consumption problem of the formation establishment, starting from the fly around equation, using double-impulse, the optimization is simulated based on the Niche Genetic Algorithm (NGA). The simulation result shows that the NGA is a good way to solve the fuel consumption optimization problem of formation establishment, and the fuel consumption when the phase determined is greater than the fuel consumption when the phase not determined.

In order to solving the establishment problem of satellites formation establishing in elliptical orbits, the problem of Lambert transfer based on two-impulse control with unfixed time and initial position is studied. The Niche Genetic Algorithms (NGA) is used to locating the optimal transfer orbit, and the transfer time and initial position are coded into genetic algorithms, as well as the fitness functions are designed to total coasting. The results show that the NGA can solve the optimization problem of two-impulse control effectively, furthermore, the performance of NGA is more effective than the traditional genetic algorithm. The research provides a way for satellites formation establishing based on two-impulse control.

Cotton fabrics pretreated with cationic polyacrylamide with quaternary ammonium group were dyed with three reactive dyes in the absence of salt by cold pad-batch dyeing method. The influences of various conditions in the dyeing process, including urea dosage, selection of alkali agent and its dosage, batching time and batching temperature on colour yield, were analyzed. Fastness properties of the dyed cotton and the dye penetration were investigated between the pretreated and untreated cotton. The results showed that the dyesK/Svalues of the pretreated cotton were improved compared with those of the untreated one, in addition, the penetration and fastness properties of the dyed cationic cotton were satisfied for application.

The cationic cotton was obtained by pretreatment with tertiary amine cationic polyacrylamide, and dyed with various active groups reactive dyes in the absence of salt. The influences of reactive dye structures on dyeing properties were analyzed and the results showed that types of active groups in reactive dyes had little effect on dye exhaustion, but it was quite relative with dye reactivity and fixation. Reactive dyes with low molecular weight and more anionic groups were adsorbed more quickly on the cationic cotton and their adsorption time to reach adsorption equilibrium was shorter, compared with that with large molecular weight and less anionic groups. Moreover, exhaustion of reactive dyes with large molecular structures decreased due to sterically hindered effect in the salt-free dyeing. The results also displayed that the monochlorotriazine reactive dyes and the vinyl-sulfone ones were favorable to dye the cationic cotton without salt addition.

The subject of the paper is to study the effect of the different river habitat on biological diversity of microalgae in order to reveal the status of algae indicators in the evaluation of river ecosystem health. The study is conducted to compare planktonic algae and epilithic algae in Shendinghe rive (SR) and Qihe river (QR) of Danjiangkou reservoir in August 2010. The trophic status is evaluated by saprobic system. There are 193 species (varieties) belonging to 98 genera and 7 phylums of planktonic algae and 61 species (varieties) belonging to 36 genera and 3 phylums of epilithic algae in the samples of SR. There exist 165 species (varieties) belonging to 82 genera and 7 phylums of planktonic algae and 48 species (varieties) belonging to 10 genera and 4 phylums of epilithic algae in the samples of QR. 15 genera instructions 21 species (varieties) of saprobic indicators are identified in the monitored rivers. The number of oligonucleotide indicators in QR is higher than that in SR, and the number of eutrophication indicators in QR is lower than that in SR. The water quality of QR is Biologically superior to that of SR.

Aims Fine-roots (≤2 mm diameter) are important in controlling energy and matter exchange between tree and soil. Fine-root longevity is a critical determinant of fine-root turnover and therefore carbon allocation and nutrients returned to the soil. However, little is known about variation in fine-root longevity in relation to spatial and temporal heterogeneity in availability of soil resources (such as temperature, moisture and nitrogen). How does availability of soil resources affect fine root longevity? Do different tree species, soil depths and root cohorts have different fine-root longevity in the same forest site? To answer these questions it is important to understand the fate of fine roots in soil with heterogeneous resource availability. The objectives of this study were to: 1) compare fine-root longevity between ash (Fraxinus mandshurica) and larch (Larix gmelinii) in the same site, 2) define patterns of fine-root longevity in different soil depths and elucidate the effect of availability of soil resources on fine-root longevity and 3) analyze seasonal changes of fine-root longevity for both species. Methods Mini-rhizotrons (root observation tubes) were used to estimate fine-root longevity. We established three 20 m×30 m plots in an ash and larch plantation, and installed six clear PVC (polyvinyl chloride) mini-rhizotron tubes (90 cm long × 5.5 cm inside diameter) in each plot in October 2003. From 16 April to 30 October in 2004, video images were collected from the mini-rhizotron tubes at approximately two-week intervals and analyzed with an image analysis system (RooTracker software). We compared differences in longevity between tree species, soil depths and seasonal root cohorts using cumulative survival rate and median root longevity (MRL) of fine roots by Kaplan-Meier methods in survival analysis. Important findings Cumulative survival rate of fine roots decreased gradually with time. Survivorship curves showed that cumulative survival rate of fine roots in ash was significantly higher than that in larch (p 0.001 ); MRL was 111±7 d in ash and 77±4 d in larch. Higher fine root mortality was found in 0-20 cm soil, as MRL was 62±11 d for larch and 111±6 d for ash, contrasting with 95±11 d for larch and 124±20 d for ash in deep soil. This study also indicated longer lifespans of fine roots produced in summer (82±6 d for larch) than spring (47±13 d). Similar seasonal pattern was found for ash. Fine-root longevity was different between tree species due to different root genetics, physiology and architecture. High soil temperature and high N content can decrease fine root longevity. In summer, photosynthates are allocated to roots and fine roots grow rapidly and have increased longevity. Our results suggested that fine-root longevity is correlated with genetics of the species, physiological status and soil resource availability.

Root observation tubes (minirhizotrons) were used to study the effects of nitrogen addition on the fine root growth of Fraxinus mandshurica and Larix gmelinii, with the correlations between the fine root lifespan and nitrogen availability analyzed. After the nitrogen addition, the fine root number of F. mandshurica and L. gmelinii had a decreasing trend, but the fine root diameter became larger and the branching ratio decreased. The survival rate of F. mandshurica fine roots increased, and the median root lifespan prolonged 105 days, compared with the control. No significant responses to the nitrogen addition were observed in the survival rate of L. gmelinii fine roots. The first-order fine roots with smaller diameter, the roots in surface soil (0-15 cm), and the fine roots newly born in spring and summer were vulnerable to extend their lifespan by nitrogen addition, suggesting that the fine roots with higher physiological activity were easily to be affected by nitrogen fertilization.

When a new person faces before a tensor-based face recognition system, this person is unable to be recognized, since this person's identity subspaces is not contained in the training data. Although PCA method can figure out this problem by adding new image to the training data, but it cannot maintain the original tensor framework and the merit of multi-factor analysis. In this paper, incremental tensor data by facial synthesis estimating is proposed for face recognition. To make full use of the information of new input person in the tensor framework, facial expression synthesis method is used to estimate the missing tensor data. Then the new tensor is constructed, and the subspace of the new person could be constructed based on the new tensor. Thus, the tensor framework can be used to carry on face analysis of the new person, including face recognition. The experimental results show that the proposed method has average 20.1% higher rate for face recognition compared with batch PCA method.

With 16 years old Larix kaempfersoil plantation in the mountainous area of eastern Liaoning Province as test object, this paper studied the effects of fertilization on the fine root diameter, root length, and specific root length (SRL) of the first to fifth order roots. The results showed that with ascending root orders, the mean fine root diameter and root length increased, while the SRL decreased significantly. Among the five order roots, the first order roots were the thinnest in diameter, the shortest in length, and the highest in SRL, but the fifth order roots were in reverse. The variance coefficients for the fine root diameter, root length, and SRL increased from the first to the fifth order roots. Except for the first order roots, soil depth had no significant influence on the fine root diameter, root length and SRL. Fertilization affected the fine root diameter, root length, and SRL of the first and the second order roots significantly, hut had little effects on other order roots. N fertilization decreased the mean diameter of the first and the second order roots significantly, and N or N + P fertilization decreased the mean length of the first order roots in surface soil (0-10 cm) significantly. The SRL of the first order roots in surface soil increased significantly under N fertilization.

To investigate the prevalence of domestic violence (DV) in Hunan.Using a multi-stage sampling strategy, 9451 households involving 32 720 persons in urban, rural and industrial areas in Hunan, China were studied. Multiform clue investigation and face-to-face interviews were combined to investigate the prevalence of DV.A lifetime prevalence of DV was reported by 1533 households (16.2%). A total of 1098 households (11.6%) reported at least one incident of DV in the previous year. Both lifetime and 12-month prevalence of DV varied significantly by geographic setting (P0.01). The lifetime prevalence abuse rates were: spousal 10.2%, child abuse 7.8%, and elder 1.5%. With regard to household structure, the lifetime prevalence of DV was highest among those remarried families (21.0%), followed by married couples with one child and extended families with several generations living together (20.1% and 20.0%, respectively). The highest rate of spousal abuse was found among remarried families (14.7%), while child and elder abuse was most prevalent among extended families (12.4% and 4.1%, respectively).The findings suggested that although the prevalence of DV in Hunan was modest compared to Western countries, it remained a serious public health problem affecting over 1 in 10 households. Furthermore, the prevalence of various types of DV varied by geographic setting and family structure, suggesting that diverse geographic setting and family constellations carried different risk and protective features.