1. Circulating microparticles, protein C, free protein S and endothelial vascular markers in children with sickle cell anaemia
- Author
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Andrea Piccin, Ciaran Murphy, Elva Eakins, Jan Kunde, Daisy Corvetta, Angela Di Pierro, Giovanni Negri, Mazzoleni Guido, Laura Sainati, Corrina Mc Mahon, Owen Patrick Smith, and William Murphy
- Subjects
sickle cell anaemia ,microparticles ,protein C ,protein S ,adrenomedullin ,endothelin-1 ,nitric oxide ,Cytology ,QH573-671 - Abstract
Introduction: Circulating microparticles (MP) have been described in sickle cell anaemia (SCA); however, their interaction with endothelial markers remains unclear. We investigated the relationship between MP, protein C (PC), free protein S (PS), nitric oxide (NO), endothelin-1 (ET-1) and adrenomedullin (ADM) in a large cohort of paediatric patients. Method: A total of 111 children of African ethnicity with SCA: 51 in steady state; 15 in crises; 30 on hydroxyurea (HU) therapy; 15 on transfusion; 17 controls (HbAA) of similar age/ethnicity. MP were analysed by flow cytometry using: Annexin V (AV), CD61, CD42a, CD62P, CD235a, CD14, CD142 (tissue factor), CD201 (endothelial PC receptor), CD62E, CD36 (TSP-1), CD47 (TSP-1 receptor), CD31 (PECAM), CD144 (VE-cadherin). Protein C, free PS, NO, pro-ADM and C-terminal ET-1 were also measured. Results: Total MP AV was lower in crisis (1.26×106 ml−1; 0.56–2.44×106) and steady state (1.35×106 ml−1; 0.71–3.0×106) compared to transfusion (4.33×106 ml−1; 1.6–9.2×106, p0.9, p
- Published
- 2015
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