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A new kind of bio-based polyamide 5,6 (PA56) fiber has been synthesized from adipic acid and 1,5-pentanediamine. In this report, the characterization, antistatic treatment, mechanical properties, m...

In this paper, a green solvent for chitosan, plasma acid, was made and chitosan/plasma-acid solutions were prepared. An environmentally friendly chitosan/polyvinyl alcohol (PVA) nano-membrane was p...

To analyze the clinical characteristics, diagnosis and prognostic factors of bone marrow necrosis (BMN) patients, aim to avoid misdiagnosis, missed diagnosis or delayed treatment.The clinical data of 51 BMN patients treated in the Affiliated Hospital of Xuzhou Medical University from January 2010 to December 2017 were retrospectively analyzed. The types of primary disease, etiology, clinical manifestations, laboratory tests, radiological findings, treatment outcomes and prognostic factors were summrized, and the reasons for misdiagnosis were analyzed.Among 51 BMN patients, the hematologic tumor was detected out in 32 patients; solid tumors caused- BMN was detected out in 14 patients, benign lesions for 5 patients. The time of interval from the appearance of symptoms to the confirmation of BMN was 7 days to 6 months, with a median of 35 days. Misdiagnosis and missed diagnosis occurred in 25.5% of the BMN patients. Anemia was found in all of the 51 BMN patients, fever accounted for 58.8%, systemic bone pain for 52.9%, bleeding for 29.4%, lymphadenectasis for 37.3%, and hepatosplenomegaly for 19.6%. Leukoerythroblastic anemia accounted for 84.3%, bicytopenia for 51.0%, pancytopenia for 25.5%, and monocytopenia for 23.5%. The serologic test revealed no specific results. The first bone marrow aspiration were 38 patients and multi-site puncture were 7 patients. The diagnostic coincidence rate of bone marrow smear was 88.2%. Among 51 BMN patients, 41 patients received bone marrow biopsy, and the diagnostic coincidence rate of bone marrow biopsy was 75.6%. The abnormal signals were found in multiple vertebral bodies by spinal/pelvic MRI scan in 13 BMN patients; PET-CT scan revealed a diffuse pattern of low FDG uptake in the bone marrow in 16 patients, with a local increase in FDG uptake accompanied by bone marrow involvement. For 46 patients with BMN combined with malignancies, among which 35 patients died (76.1%) and the median survival time was 25 days. Among the 32 patients with hematologic tumors, early death occurred in 12 patients, BMN disappeared in 11 out of 20 patients received active chemotherapy for the primary disease, 9 patients died within 1 week to 3 months. Fourteen patients combined with bone marrow metastatic carcinoma died within 2 weeks to 3 months. Focal necrosis disappeared in 4 out of 5 BMN patients secondary to non-malignant diseases after symptomatic supportive treatment and still alived. Multiple logistic regression was performed to analyze factors affecting the prognosis of BMN patients, the result showed that the prognosis of BMN was closely related to the factors of primary disease (benign and malignant). The reasons for misdiagnosis and missed diagnosis were as follows: hidden onset of the primary disease, nonspecific symptoms, insufficient understanding and alertness of the physicians regarding the primary clinical characteristics and hematological abnormalities, and failure to receive multiple sites bone marrow punctures or bone marrow biopsies.BMN usually occurs concomitantly to hematologic tumors and bone marrow metastases from solid tumors. Its prognosis is closely related to the nature and severity of the primary disease and its own severity. In the clinic, BMN should be suspected in patients with severe bone pain, fever, hepatosplenomegaly, hemocytopenia, lymphadenectasis and leukoerythroblastic anemia. Bone marrow puncture at multiple positions and bone marrow biopsy can compensate for each other in the diagnosis of BMN. The combined use of the two methods can improve the diagnostic coincidence rate of BMN, and the positive rate of the etiological diagnosis of BMN.骨髓坏死患者的临床特点、预后因素及误诊分析.分析骨髓坏死（BMN）患者的临床特征、诊断及预后，避免误诊、漏诊或延误治疗。.回顾性分析2010年1月至2017年12月徐州医科大学附属医院收治的51例BMN患者的临床资料，总结其原发病种类、病因、临床表现、实验室检查及影像学表现、治疗转归及预后影响因素，并对误诊原因进行分析。.51例BMN患者中，原发病为血液肿瘤32例，实体肿瘤所致骨髓转移癌14例，良性病变者5例。患者从出现症状到确诊BMN的时间为7 d-6 个月，中位时间为35 d。25.5% BMN患者存在误诊和漏诊。51例BMN患者中，贫血占100%，发热占58.8%，全身骨痛占52.9%，出血占29.4%，淋巴结肿大占37.3%，肝脾肿大占19.6%。幼红-幼粒细胞贫血占84.3%，二系细胞减少占51.0%，全血细胞减少占25.5%，单系细胞减少占23.5%。血清学检查无特异性。1次骨髓穿刺确诊者38例，多部位穿刺确诊者7例，骨髓涂片的诊断符合率为88.2%。51例患者中，41例行骨髓活检，骨髓活检的诊断符合率为75.6%。13例BMN患者脊柱/骨盆MRI显示多发椎体异常信号，16例患者的PET-CT显像示骨髓弥漫性摄取FDG低下，骨髓浸润时伴局部摄取增高。46例合并BMN恶性疾病的患者中，35例死亡（76.1%），中位生存期为25 d。32例血液肿瘤患者中，12例早期死亡，20例针对原发病积极化疗后，11例BMN消失，9例于1周-3个月内死亡。14例骨髓转移癌合并BMN患者于2周-3个月内死亡。5例继发于非恶性疾病的BMN患者，4例经对症支持治疗，坏死灶消失，现仍存活。对BMN患者预后的影响因素进行多因素Logistic回归分析，结果显示，BMN预后与原发病因素（良恶性）密切相关。 误、漏诊主要原因：原发病隐匿，症状无一定规律，对主要临床特点和血液学异常缺乏认识和警惕性，未及时行多部位骨髓穿刺和骨髓活检等。.BMN主要伴发于血液肿瘤及实体瘤骨髓转移，其预后与原发病性质及本身危重性密切相关，临床上对剧烈骨痛、发热、肝脾淋巴结肿大、血细胞减少伴幼红-幼粒细胞贫血者，应高度警惕BMN的存在。多部位骨髓穿刺和骨髓活检可相互弥补各自不足，二者联合检查可以提高BMN诊断符合率和病因诊断阳性率。.

We determined whether genetically engineered immature dendritic cells (imDCs) mediated by lentiviral vectors alleviate acute graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation (allo-BMT) in mice. We introduced the mouse chemokine receptor 7 (Ccr7) gene into the bone marrow-derived imDCs of C57BL/6 mice to construct genetically engineered imDCs. A 1:1 mixture of bone marrow and spleen cells from the donors was injected into the recipients, which were divided into four groups: radiation, transplantation, empty vector, and transgenic imDC groups. Symptoms, clinical scores, GVHD pathological changes, and survival times and rates of recipients were recorded; secretion of IFN-γ and IL-4, and allogeneic chimerism rates were detected. The survival time of the transgenic imDC group (27.5 ± 7.55 days) was significantly longer than in the other three groups (P < 0.01). The GVHD score of the imDC group mice was significantly lower than in the transplantation and empty vector groups (P < 0.05), which meant that mice in the transgenic imDC group had the lightest pathology damage in the target organs. In the transplantation group, IFN-γ increased while IL-4 decreased. In contrast, IFN-γ decreased and IL-4 increased in both empty vector and trans-imDC groups, and the difference was significant in the latter (P < 0.01). Thirty days or more following transplantation, the allogeneic chimerism rate was still 95-100%, suggesting complete donor type implantation. Ccr7 transfection into imDCs suppressed occurrence and severity of acute GVHD after allo-BMT in mice; the mechanism might be associated with IFN-γ decrease and IL-4 increase.

The aim of the present study was to investigate the protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax. Thirty-six SD rats were randomly divided into three groups (n=12) including sham operation (S) group, ischemia-reperfusion group (I/R) group and ischemic preconditioning (IP) group. After anesthesia with intraperitoneal injection of chloral hydrate, bilateral renal pedicles were clipped for 45 min, followed by perfusion for 6 h to establish the I/R model. Both kidneys in rats of S group were separated and exposed for 45 min, but renal pedicles were not clipped. In IP group, bilateral renal pedicles were clipped for 5 min, followed by perfusion for 5 min, this procedure was repeated 3 times. Then bilateral renal pedicles were clipped for 45 min, followed by perfusion for 6 h. Blood samples were collected and rats were sacrificed to collect renal tissue. Levels of serum creatinine (Cr) and blood urea nitrogen (BUN) were measured. Activity of superoxide dismutase (SOD) was measured by xanthine oxidase assay. Degree of renal injury was evaluated by H&E staining. TUNEL kit was used to detect the number of apoptotic cells in renal tissue. Expression levels of Bcl-2 and Bax were detected by semi-quantitative PCR and western blot analysis at mRNA and protein levels, respectively. Results showed that levels of Cr and BUN in I/R and IP groups were significantly higher than those in S group, and levels of Cr and BUN in I/R group were significantly higher than that in IP group (P

This paper develops a model to simulate wire-to-sheet lapped resistance microwelding (RMW) process. The model employs a coupled thermal–electrical–mechanical analysis and accounts for temperature-dependent thermophysical properties of materials, contact resistance and the Peltier effect. Results show the interface temperature between the molybdenum electrode and copper wire is higher than the faying surface. The predicted thermal distributes agree well with the experimental data. The proposed model can be applied to predict the effects of the welding parameters.

An optimization algorithm of urban traffic signal coordinated control with bus priority, which is aiming at achieving optimal comprehensive traffic efficiency, is proposed in this paper. Six kinds of key parameters that dominate signal control effects are extracted from abundant factors. Based on these parameters, an advance and flexible coding scheme which can generate detail signal control information in QGA is put forward. Considering travel time, no wait passing rate, crossing delay, green wave effect and other factors, a new fitness function is constructed. Verified by simulations in PARAMICS, the optimized signal control scheme can obviously improve comprehensive regional traffic efficiency.

In this study, nitrogen-doped ZnO (N-doped ZnO) nanocrystallites with a one-dimensional structure were synthesized successfully via an advanced wet chemical technique, and their microstructures were characterized by SEM, HRTEM, XRD, and XPS. The catalytic performance of the as-synthesized samples was evaluated by investigating their effect on the thermal decomposition of ammonium perchlorate (AP) by DSC and TG. Results indicate that the morphologies of N-doped ZnO nanocrystallites mainly depend on the presence of urea in the raw materials. Nanocrystallites with peculiar morphology, in which numerous nanorods with a diameter of 40–50 nm arrange orderly and symmetrically in hollow nanotubes with a diameter of 200–800 nm and thickness of 20–30 nm, can be produced when urea is used as a raw material. The as-synthesized N-doped ZnO sample with peculiar morphology drives the thermal decomposition peak of AP decrease about 163 °C with a strong decomposition heat about 1,325 J/g, and the activation energy also decreases from 178.22 to 93.51 kJ/mol. The enhanced catalytic activity of N-doped ZnO sample can be attributed to oxygen vacancies and other defects induced by the doping of nitrogen.

To investigate the effects of immature dendritic cells (imDC) expressing chemokine receptor-7 (CCR7) on acute graft-versus-host disease (aGVHD) in allogeneic bone marrow transposed (allo-BMT) mouse model.We constructed the lentiviral vectors carrying mouse CCR7 gene and infect imDC effectively in vitro. GVHD model was established with C57BL/6(H-2b) donor mice and BALB/c (H-2d) recipient mice. After irradiation, recipients were injected with donor bone marrow and spleen cells along with CCR7-modified dendritic cells. Mice were randomized into irradiation, transplant control, pXZ9-imDC (empty vector control) and CCR7-imDC groups. Survival, GVHD score, histopathological analysis and plasma levels of inflammatory cytokines were observed.The mean survival in irradiation, transplantation, pXZ9-imDC and CCR7-imDC groups were (8.20±1.48)d, (12.20±2.78)d, (20.70±6.01)d and (27.5±7.55)d respectively. The survival in CCR7- imDC group was significantly improved compared with other groups (P0.05). GVHD scores in transplantation, pXZ9-imDC and CCR7-imDC groups were (6.90±1.66), (5.60±0.97) and (4.10±1.79) respectively. CCR7-imDC group had significantly lower GVHD score and minor tissue damages shown by histopathological analysis than the other groups. Plasma IFN-γ level increased and reached the peak at +10 day in transplant group, while it gradually decreased in pXZ9-imDC and CCR7-imDC groups, and then reached the nadir at +20 day post-allo-BMT, with the lowest level in CCR7-imDC group (P0.01). Plasma IL-4 decreased in transplant group, while it gradually increased in pXZ9-imDC and CCR7-imDC groups and reached the highest level at + 10 day in CCR7- imDC group (P0.01). The 95%-100% of H-2b positive cells in recipient mice on + 30 day post-allo-BMT demonstrated the complete donor- type implantation.Genetically modified immature DC by CCR7 gene could alleviate damages by GVHD and prolong survival of recipient mice after allo-BMT.

To explore the prevention and management of human cytomegalovirus (HCMV) infection accompanied with acute pancreatitis after kidney transplantation.A retrospective analysis of 5 patients with acute pancreatitis after kidney transplantation was conducted. The incidence of acute pancreatitis after kidney transplantation was 2.3% (5/217). All the 5 cases were complicated by active HCMV infection, 3 of which had hyperlipemia and 2 had liver dysfunction. Three cases were finally cured while the other 2 died, one of which was due to respiration failure arising from HCMV interstitial pneumonia accompanied with hemorrhagic necrotizing pancreatitis, and the other due to fulminating liver function failure because of active HCMV infection accompanied with hemorrhagic necrotizing pancreatitis.Active HCMV infection is the most important factor responsible for acute pancreatitis, and early diagnosis and treatment are crucial to lower mortality rate.

Estrogen is a strong growth regulator of the prostate stromal cells, it produces a marked effect by means of binding to specific receptors. Many researches have indicated that estrogen and estrogen receptors take part in the whole process and development of benign prostatic hyperplasia. This article gives a general description of the function of estrogen and estrogen receptors in benign prostatic hyperplasia.