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The recent discovery of circular RNAs (circRNAs) in serum exosomes suggests a novel and potentially useful tool for noninvasive cancer diagnosis. However, there are currently no substantial studies addressing this topic. RNA-sequencing analysis was performed between three pairs of non-small-cell lung cancer (NSCLC) patients and controls. The diagnostic values of exosomal circRNAs were assessed in a training set, validation set 1, and validation set 2. A total of 46 abnormally expressed circRNAs were identified. Among these, circ_0047921, circ_0056285, and circ_0007761 were found to display significant diagnostic validity for NSCLC. In distinguishing NSCLC cases from healthy controls, the panel of aforementioned circRNAs exhibited area under the curve (AUC) values of 0.926 (95% CI, 0.895-0.956) in the training set and 0.919 (95% CI, 0.877-0.962) in validation set 1. Circ_0047921 could distinguish NSCLC cases from chronic obstructive pulmonary disease controls (AUC, 0.890; 95%CI, 0.831-0.948), whereas the circ_0056285 and circ_0007761 combination could distinguish NSCLC cases from tuberculosis controls (AUC, 0.820; 95% CI, 0.739-0.902). For an early NSCLC diagnosis, similar results were observed for these circRNAs in distinguishing early-stage NSCLC cases from healthy controls, chronic obstructive pulmonary disease controls, or tuberculosis controls. Circ_0056285 expression was correlated with clinical stages and lymph node metastasis. The exosomal circRNAs, circ_0047921, circ_0056285, and circ_0007761, are promising biomarkers for NSCLC diagnosis in the Chinese population.

Objective: Abundant evidence has illustrated that long non-coding RNA (lncRNA) plays a vital role in the regulation of tumor development and progression. Ectopic expression of a novel lncRNA, termed lnc-AGER-1, has been discovered in cancers, and this lncRNA was reported to exert an anti-tumor effect. However, its biological mechanism remains unelucidated in colorectal cancer. Methods: A total of 159 paired colorectal cancer specimens and adjacent tissues was applied to detect the expression of lnc-AGER-1 by the quantitative Real-time PCR (qRT-PCR), and a series of functional assays was executed to uncover the role of this lncRNA on colorectal cancer. Results: We found that the expression of lnc-AGER-1 in the tumor tissues was significantly down-regulated, while compared with adjacent normal tissues (0.0115 ± 0.0718 vs. 0.0347 ± 0.157; P < 0.0001). Also, lnc-AGER-1 was observably associated with clinical T status (r = −0.184, P = 0.024). Patients with advanced T status exerted a significantly lower level of lnc-AGER-1 than those with early T status (20.0% vs. 40.7%, P = 0.021). Over-expression of lnc-AGER-1 inhibited cell proliferation and migration efficiency, and induced cell cycle arrest at the G0/G1 phase, and promoted cell apoptosis. Further research proved that lnc-AGER-1 altered the expression of its neighbor gene, AGER, through acting as a competing endogenous RNA for miR-182 in colorectal cancer. Conclusion: lnc-AGER-1 has a suppressive role in colorectal cancer development via modulating AGER, which may serve as a target for colorectal cancer diagnosis and treatment.

Background Long noncoding RNAs (lncRNAs) are emerging as master regulators for gene expression and thus play a vital role in human tumorigenesis and progression. But the involvement of novel lncRNAs in non-small cell lung cancer (NSCLC) remains largely unelucidated. Methods A total of 170 NSCLC and their adjacent non-tumor tissues were enrolled to detect the expression of Lnc-LSAMP-1 by RT-qPCR. The effects of Lnc-LSAMP-1 on cell proliferation, migration, invasion and drug-sensitivity were determined by in vitro and in vivo experiments. The proteins that interact with Lnc-LSAMP-1were confirmed by RNA pull-down assay. RNA-sequencing were used to identify the potential targets of Lnc-LSAMP-1 in NSCLC. Results We found that Lnc-LSAMP-1 was significantly down-regulated in 170 cases of NSCLC tissues when compared to their adjacent non-cancerous tissues. Loss expression of Lnc-LSAMP-1 was notably correlated with unfavorable prognosis of NSCLC patients. The ectopic expression of Lnc-LSAMP-1 drastically inhibited lung cancer cell proliferation, viability, invasion and migration ability, arrested cell cycle and facilitated apoptosis. Chemotherapy sensitization experiments showed that over-expressed Lnc-LSAMP-1 enhanced the inhibition of cell proliferation induced by TKI. Mechanistically, Lnc-LSAMP-1-LSAMP formed a complex which could protect the degradation of LSAMP gene, and thus exerted crucial roles in NSCLC progression and TKI targeted treatment. Conclusions Consequently, our findings highlight the function and prognostic value of Lnc-LSAMP-1 in NSCLC and provide potential novel therapeutic targets and prognostic biomarkers for patients with NSCLC.

A non-invasive method to distinguish potential lung cancer patients would improve lung cancer prevention. We employed the RNA-sequencing analysis to profile serum exosomal long non-coding RNAs (lncRNAs) from non-small cell lung cancer (NSCLC) patients and pneumonia controls, and then determined the diagnostic and prognostic value of a promising lncRNA in four datasets. We identified 90 dysregulated lncRNAs for NSCLC and found the most significant lncRNA was a novel isoform of linc01125. Serum exosomal linc01125 could distinguish NSCLC cases from disease-free and tuberculosis controls, with the area under the curve values as 0.662 [95% confidence interval (CI) = 0.614–0.711] and 0.624 (95% CI = 0.522–0.725), respectively. High expression of exosomal linc01125 was also correlated with an unfavorable overall survival of NSCLC (hazard ratio = 1.48, 95% CI = 1.05–2.08). Clinic treatment decreased serum exosomal linc01125 in NSCLC patients (P = 0.036). Linc01125 functions to inhibit cancer growth and metastasis via acting as a competing endogenous RNA to up-regulate tumor necrosis factor alpha-induced protein 3 (TNFAIP3) expression by sponging miR-19b-3p. Notably, the oncogenic transformation of 16HBE led to decreased linc01125 in cells but increased linc01125 in cell-derived exosomes. The expression of linc01125 in total exosomes was highly correlated with that in tumor-associated exosomes in serum. Moreover, lung cancer cells were capable of releasing linc01125 into exosomes in vitro and in vivo. Our analyses suggest serum exosomal linc01125 as a promising biomarker for non-invasively diagnosing NSCLC and predicting the prognosis of NSCLC.

Background: A non-invasive method to distinguish potential lung cancer patients would improve lung cancer prevention. Methods: We employed RNA-Seq analysis to profile serum exosomal long non-coding RNAs (lncRNAs) from non-small cell lung cancer (NSCLC) patients and pneumonia controls, and then determined diagnostic and prognostic value of a promising lncRNA in four datasets. Findings: We identified several dysregulated lncRNAs and found that those up-regulated lncRNAs in NSCLC were mostly tumor-suppressive, while down-regulated lncRNAs were most oncogenic. The most significant lncRNA was a novel isoform of linc01125. Serum exosomal linc01125 could distinguish NSCLC cases from disease-free and tuberculosis controls, and its expression was also correlated with prognosis. Clinic treatment decreased serum exosomal linc01125 in NSCLC patients. Linc01125 functions to inhibit cancer growth and metastasis. Acting as a competing endogenous RNA, linc01125 sponges several microRNAs (miRs) to regulate gene expression such as TNFAIP3 via binding miR-19b-3p. Notably, oncogenic transformation of 16HBE leads to decreased linc01125 in cells but increased linc01125 in cells-derived exosomes. Linc01125 expression in exosomes was also highly correlated with that in tumor-associated exosomes. Linc01125 could be loaded into exosomes in vitro and in vivo, which did not dampen tumor development. Interpretation: Our analyses suggest serum exosomal linc01125 as a promising biomarker for non-invasively diagnosing NSCLC and predicting prognosis of NSCLC. Funding: National Natural Scientific Foundation of China, Guangzhou Science and Technology Program Pearl River Nova projects, The National Key Research and Development Program of China, Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program. Declaration of Interest: None to declare. Ethical Approval: The study was approved by the institutional review board of Guangzhou Medical University.

There is a perception that long noncoding RNA (lncRNA) has relationship with carcinogenesis. Many studies have previously identified and validated that the section of chromosome 11p13 is associated with high incidence of tumor. In this study, we investigated a new lncRNA, named

Antisense RNA (AS) is a type of long non-coding RNAs that functions as a post-transcriptional regulatory element on regulating parental coding gene expression via directly binding to complementary mRNA sequences. We aimed to investigate the effect of the AS to FEZF1 gene on non-small cell lung cancer (NSCLC) development. The expression level of lncRNA FEZF-AS1 and FEZF1 was determined by the quantitative Real-time PCR in 160 cases of NSCLC tissues and their adjacent non-tumour tissues. We found that lncRNA FEZF-AS1 was significantly up-regulated in tumour tissues when compared to the adjacent non-cancerous tissues (P = 0.001), and it's high expression correlated with advanced stages (P = 0.002) and Tumour Family History (P = 0.029). Meanwhile, In 58 cases of NSCLC tissues the expression of lncRNA FEZF-AS1 was positively associated with that of FEZF1expression (r = 0.8810, p = 1.6575E-20). By GEPIA database analysis, we also found that the expression of lncRNA FEZF-AS1 and FEZF1 were significantly higher in tumour tissues than those of the adjacent non-cancerous tissues in 969 NSCLC patients (P 0.05), and lncRNA FEZF-AS1 was positively correlated with FEZF1 (r = 0.90, P 0.001). These results suggest that lncRNA FEZF-AS1 relate to the progression of lung cancer patients and it may be a potential target for cancer therapy.

Abundant evidence has illustrated that long non-coding RNA (lncRNA) plays a vital role in the regulation of tumor development and progression. Ectopic expression of a novel lncRNA, termedA total of 159 paired colorectal cancer specimens and adjacent tissues was applied to detect the expression ofWe found that the expression of

Background There is very little known about how long non-coding RNAs (lncRNAs) are associated with membrane proteins in lung cancer. The limbic system-associated membrane protein ( LSAMP ) has been reported to play a tumor suppressor role in a variety of cancers.Methods We aimed to explore the lncRNA associated with LSAMP and explore its effects on lung cancer.Results We found that Lnc-LSAMP-1 was significantly down-regulated in 170 cases of lung tumor tissues when compared to the adjacent non-cancerous tissues (p

Background: Recent discovery of tumor-special circRNAs in serum exosomes have suggested novel and worth considering biomarkers for cancer non-invasive diagnosis. There is not yet any research literature targeting this topic. Methods: We conducted RNA-seq analysis to characterize circRNA profiles of serum exosomes between 3 pairs of non-small cell lung cancer (NSCLC) patients and ruled-out cases of suspected lung cancer, and then assessed diagnostic value of differentially expressed circRNAs in a training set including 120 NSCLC patients and 46 tuberculosis plus 94 healthy controls and a validation set with a total of 63 NSCLC cases and 58 chronic obstructive pulmonary disease plus 95 healthy controls. Findings: We delineated the circRNA profile in serum exosomes of NSCLC and identified 46 abnormally expressional circRNAs. The circ_0047921, circ_0056285 and circ_0007761 were further found to exert a considerable accuracy for diagnosing NSCLC in both the training set (area under curve [AUC] = 0.806, 95% confidence interval [95%CI] = 0.753-0.859) and the validation set (AUC = 0.810, 95%CI = 0.772-0.844). Their panel also played a significant role in diagnosis of early-stage NSCLC (AUC = 0.773, 95%CI = 0.725-0.815). Furthermore, circ_0047921 and circ_0007761 expressions were recovered to normal level after treatment. Circ_0056285 expression was correlated with clinical stage and lymph node metastasis. Interpretation: These findings suggested the exosomal circRNAs, circ_0047921, circ_0056285 and circ_0007761 to be promising biomarkers for NSCLC diagnosis as well as early diagnosis. Funding Statement: This study was supported by the National Natural Scientific Foundation of China grants 81402753, 81672303 (L. Yang) and by 81473040, 81673267 (J. Lu). Guangzhou Science and Technology Program Pearl River Nova projects Grant 201710010049 (L. Yang) and Guangzhou Education Bureau Major Projects Grant 1201610122 (L. Yang). Declaration of Interests: The authors declare that they have no competing interests. Ethics Approval Statement: The study was approved by the institutional review board of Guangzhou Medical University