Search

Thinning of the sclera happens in myopia eyes owing to extracellular matrix (ECM) remodeling, but the initiators of the ECM remodeling in myopia are mainly unknown. The matrix metalloproteinase (MMPs) and tissue inhibitors of matrix metalloproteinase (TIMPs) regulate the homeostasis of the ECM. However, genetic studies of the MMPs and TIMPs in the occurrence of myopia are poor and limited. This study systematically investigated the association between twenty-nine genes of the TIMPs and MMPs families and early-onset high myopia (eoHM) based on whole exome sequencing data. Two TIMP4 heterozygous loss-of-function (LoF) variants, c.528C>A in six patients and c.234_235insAA in one patient, were statistically enriched in 928 eoHM probands compared to that in 5469 non-high myopia control (p = 3.7 × 10−5) and that in the general population (p = 2.78 × 10−9). Consequently, the Timp4 gene editing rat was further evaluated to explore the possible role of Timp4 on ocular and myopia development. A series of ocular morphology abnormalities in a dose-dependent manner (Timp4−/− < Timp4+/− < Timp4+/+) were observed in a rat model, including the decline in the retinal thickness, the elongation in the axial length, more vulnerable to the form deprivation model, morphology changes in sclera collagen bundles, and the decrease in collagen contents of the sclera and retina. Electroretinogram revealed that the b-wave amplitudes of Timp4 defect rats were significantly reduced, consistent with the shorter length of the bipolar axons detected by HE and IF staining. Heterozygous LoF variants in the TIMP4 are associated with early onset high myopia, and the Timp4 defect disturbs ocular development by influencing the morphology and function of the ocular tissue. [ABSTRACT FROM AUTHOR]

A personal mobility system with a self-adjusting center of gravity is designed to aid agricultural work. This personal mobility system can assist elderly and disabled people with mobility problems to perform simple agricultural tasks. Simulations and experiments were performed using a 2.5-times reduced model of the personal mobility system, and the feasibility and effectiveness of the proposed system were verified. With the initial position of the center of gravity of the personal mobility system as the set value, the proportional-integral-derivative control of the counterweight motion is coordinated horizontally using two corresponding motors and the center of gravity of the system is controlled within a certain range of the set value. The experimental transition time is 10.8 s and the simulated transition time is 10.4 s. The error between the two is 3.85 %. The results demonstrate that the proposed adaptive control system achieves adaptive adjustment of the center of gravity. [ABSTRACT FROM AUTHOR]

Familial exudative vitreoretinopathy (FEVR) is a severe clinically and genetically heterogeneous disease that is characterized by vascular disorder. FEVR exhibits strikingly variable clinical phenotypes, ranging from asymptomatic to total blindness. In this case, we present a patient who was first treated as having high myopia and retinopathy but was finally diagnosed with FEVR caused by the heterozygous deletion of exon 7 in TSPAN12 with the aid of whole genome sequencing (WGS). Typical vascular changes, including vascular leakage and an avascular zone in the peripheral retina, were observed in the proband using fundus fluorescein angiography (FFA), and the macular dragging was shown to be progressing in the follow-up visit. Furthermore, the proband showed unreported TSPAN12-related phenotypes of FEVR: ERG (full-field electroretinogram) abnormalities and retinoschisis. Only mild vascular changes were exhibited in the FFA for the other three family members who carried the same deletion of exon 7 in TSPAN12. This case expands our understanding of the phenotype resulting from TSPAN12 mutations and signifies the importance of combining both clinical and molecular analysis approaches to establish a complete diagnosis. [ABSTRACT FROM AUTHOR]

The study intends to investigate the association between family support and older adult health as well as the interaction between family support and living arrangements on their health. Cross-sectional study. Samples included in the final analysis (N = 11,430) come from the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS). Multiple logistic regression analysis was used to analyze the associations between family supports, multiplicative interaction of family supports and living arrangements, and self-rated health change. Subgroup analysis on disabled older adults was supplemented. Older adult individuals who received functional support, provided financial support, and had frequent emotional communication with their children in the past year reported better self-rated health. Moreover, having frequent emotional communication with children could bring better self-rated health for the older adults living with spouses and children (OR better vs same = 2.765, P <.01) and empty nesters who lived without children (OR better vs same = 1.551, P <.05). Our findings imply that functional support and emotional support may play an increasingly important role in the health of Chinese older individuals. The interaction between emotional support and 2 living arrangements mentioned above is relevant to better health of older individuals. We advocate for culturally tailored Age-Friendly Communities augmenting the geriatric health care framework. While bolstering social support for seniors, prioritizing fundamental needs is paramount for those with disabilities. [ABSTRACT FROM AUTHOR]

Placement for very large-scale integrated (VLSI) circuits is one of the most important steps for design closure. We propose a novel GPU-accelerated placement framework DREAMPlace, by casting the analytical placement problem equivalently to training a neural network. Implemented on top of a widely adopted deep learning toolkit PyTorch, with customized key kernels for wirelength and density computations, DREAMPlace can achieve around 40 × speedup in global placement without quality degradation compared to the state-of-the-art multithreaded placer RePlAce. We believe this work shall open up new directions for revisiting classical EDA problems with advancements in AI hardware and software. [ABSTRACT FROM AUTHOR]

Growth differentiation factor 11 (GDF11) is member of the transforming growth factor β (TGF-β) superfamily of proteins. Circulating GDF11 concentrations appear to decline with age, and its depletion is associated with cardiac hypertrophy and other morbidities. Knowledge of GDF11 regulation is limited, and the effects of natural genetic variation on GDF11 levels are currently undefined. We tested whether genetic background determines serum GDF11 concentrations using two classical inbred mouse strains: C57BL/6J (B6) and BALB/cByJ (BALB). B6 mice exhibited significantly higher GDF11 levels than BALB mice, and these strain differences were consistent throughout the life span. Overall, interactions between age and genetic background determined GDF11 concentrations, which were unaffected by sex. We then surveyed a panel of 22 genetically diverse inbred mouse strains and discovered a sixfold range in GDF11 levels at middle age. We estimated that 74.52% of phenotypic variation in GDF11 levels was attributable to genetic background. We used the Mouse Phenome Database to screen for phenotypes that correlate with GDF11. Interestingly, GDF11 levels predicted median strain life spans. This study revealed high heritability of GDF11 levels. Furthermore, our correlative data suggest that GDF11 may serve as a novel predictor of mammalian life span. [ABSTRACT FROM AUTHOR]

Adipose-derived stromal cells (ADSCs) exhibit significant potential as therapeutic agents to promote tissue regeneration. Success of ADSC-based therapies is dependent upon efficient cell expansion in vitro as well as postinjection survival in the caustic milieu of damaged tissue. Genetic background regulates ADSC proliferative capacity and stress resistance, but the extent of the genetic effect size is not completely defined. The present study aimed to quantify phenotypic ranges and heritability of in vitro ADSC characteristics. ADSCs were isolated from mice representing 16 genetically diverse inbred mouse strains, including 12 classical inbred strains and four wild-derived strains. Cells were grown in vitro, and proliferative capacity and oxidative stress resistance were assessed. The fold change for ADSC growth ranged from 0.87 (BALB/cByJ) to 23.60 (POHN/DehJ), relative to original seeding density. The heritability of proliferative capacity was estimated to be 0.6462 ( p = 9.967 × 10), and this phenotype was not associated with other ADSC traits. Cell viability following HO treatment ranged from 39.81 % (CAST/EiJ) to 91.60 % (DBA/2 J), and the heritability of this phenotype was calculated as 0.6146 ( p = 1.22 × 10). Relationships between cell viability and weight of the donor fat pad were also discovered. Donor genetic background is a major determinant of in vitro ADSC phenotypes. This study supports the development of forward genetics strategies to identify genes that underlie ADSC phenotypic diversity, which will inform efforts to improve cell-based therapies. [ABSTRACT FROM AUTHOR]

Co‐free O3‐type NaNi0.5Mn0.5O2 cathode material for sodium‐ion batteries has shown great promise due to its high theoretical capacity and plentiful Na reservoir. However, the rapid capacity recession caused by harmful phase transition and large volume strain severely restricts their practical application. Herein, the obstacle is well addressed by constructing a P2&O3 biphasic structure via a customized boron‐doping strategy. The light‐weight boron doping in the interstitial position reduces the energy gap of the formation energy of P2 and O3 structure, which induces the formation of P2&O3 biphase in high Na state. In addition, the biphasic structure exhibits near zero volume strain due to the lattice interlocking effect of P2&O3, as identified by in situ X‐ray diffraction measurement. As a result, it presents a remarkable cyclability with a capacity retention of 85.2% over 1000 cycles at a high rate of 5 C. More importantly, a pouch‐type full‐cell device can exhibit a long cycling life with 70.8% capacity retention over 150 cycles at 0.1 C. This work can offer a new inspiration for designing advanced high sodium electrode materials via light element doping for future energy storage devices. [ABSTRACT FROM AUTHOR]

Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized. Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties. The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter. The films containing ε-PL showed excellent antibacterial activity against S. aureus , B. subtilis , E. coli and P. aeruginosa. In the composting experiment, the films become degraded on the seventh day, and further degraded with the growth of molds over time. The present results showed that the active films could be a potential material for food packaging. [ABSTRACT FROM AUTHOR]

Aiming at the problems such as slow traceability efficiency, poor sharing, and the difficulty of matching the throughput of a blockchain single chain structure due to the complexity of the grain food supply chain links, the large number of participants, and the large amount of data information, this paper proposes a grain food blockchain traceability information management model based on the master-slave multichain structure by analyzing the processes and data characteristics of each link in the grain food supply chain; on this basis, the PLEW consensus algorithm based on Raft + improved PoW algorithm is designed for the master chain, and the CI-PBFT consensus algorithm based on trusted information degree is designed for the slave chain. The master chain and slave chain are anchored to each other through hash locking, and the data is uploaded and queried through smart contracts. In order to verify the effectiveness of the model, the blockchain traceability system is designed and implemented based on Hyperledger Fabric2.2. At the same time, it is compared with the transaction throughput and traceability efficiency of the blockchain single chain structure. Through the safety analysis of the data information of a company in Hubei, the results show that the grain traceability system designed and implemented in this study has certain advantages over the blockchain single chain structure in all aspects. It can also solve the grain food security problems that consumers worry about, and provide reference for the research of grain blockchain traceability information management. [ABSTRACT FROM AUTHOR]