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6 results on '"Jiantao Pang"'

1. Highly sensitive voltammetric biosensor for nitric oxide based on its high affinity with hemoglobin

Author

Jiantao Pang, Xinjian Liu, Genxi Li, Chunhai Fan, and Hugo Scheer

Subjects
Detection limit, Hemeprotein, Inorganic chemistry, chemistry.chemical_element, Biochemistry, Oxygen, Amperometry, Analytical Chemistry, chemistry.chemical_compound, chemistry, Environmental Chemistry, Hemoglobin, Voltammetry, Biosensor, Heme, Spectroscopy
Abstract

Although heme protein-based, amperometric nitric oxide (NO) biosensors have been well documented in previous studies, most have been conducted in anaerobic conditions. Herein we report a novel hemoglobin-based NO biosensor that is not only very sensitive but also usable in air. The heme protein was entrapped in a sodium montmorillonite film, which was immobilized at a pyrolytic graphite electrode surface. Film-entrapped hemoglobin can directly exchange electrons with the electrode, and this process has proven to favor the catalytic reduction of oxygen. In addition, NO induced a cathodic potential shift of the catalytic reduction peak of oxygen. This potential shift was proportional to the logarithm of NO concentration ranging from 4.0 × 10−11 to 5.0 × 10−6 mol/L. The detection limit has been estimated to be 20 pM, approximately four orders lower than previously reported amperometric detectors.

Published
2004
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2. A nitric oxide biosensor based on the multi-assembly of hemoglobin/montmorillonite/polyvinyl alcohol at a pyrolytic graphite electrode

Author

Genxi Li, Chunhai Fan, Jiantao Pang, Ting Chen, and Xinjian Liu

Subjects
Hot Temperature, Manufactured Materials, Biomedical Engineering, Biophysics, Analytical chemistry, Biosensing Techniques, Nitric Oxide, Polyvinyl alcohol, Sensitivity and Specificity, chemistry.chemical_compound, Hemoglobins, Coated Materials, Biocompatible, Electrochemistry, Graphite, Pyrolytic carbon, Electrodes, Detection limit, Chemistry, Reproducibility of Results, General Medicine, Equipment Design, Equipment Failure Analysis, Montmorillonite, Linear range, Polyvinyl Alcohol, Electrode, Bentonite, Biosensor, Biotechnology, Nuclear chemistry
Abstract

Direct electron transfer of hemoglobin (Hb) can be achieved in a Hb/montmorillonite (MMT)/polyvinyl alcohol multi-assembly at a pyrolytic graphite (PG) electrode. Accordingly, a novel nitric oxide (NO) biosensor is proposed. The reduction of NO is observed at a potential of approximately -783 mV (vs. SCE) at pH 5.5. At optimum pH, this biosensor shows a wide linear range of 1.0x10(-6)-2.5x10(-4) mol/l with a detection limit of 5.0x10(-7) mol/l. The sensor-to-sensor reproducibility is good consideringmacr; a relative standard deviation of 3.5% in five independent determinations at 5.0x10(-5) mol/l NO. The modified electrode is conveniently constructed and durable in long-term operations.

Published
2003

3. A reagentless nitric oxide biosensor based on haemoglobin/polyethyleneimine film

Author

Genxi Li, Xinjian Liu, Libin Shang, and Jiantao Pang

Subjects
Inorganic chemistry, Biomedical Engineering, Bioengineering, Biosensing Techniques, Electrochemistry, Nitric Oxide, Applied Microbiology and Biotechnology, Catalysis, Hemoglobins, Drug Discovery, Animals, Polyethyleneimine, Pyrolytic carbon, Electrodes, Detection limit, Dose-Response Relationship, Drug, Chemistry, Process Chemistry and Technology, General Medicine, Hydrogen-Ion Concentration, Electrode, Molecular Medicine, Cattle, Cyclic voltammetry, Biosensor, Biotechnology, Chemically modified electrode
Abstract

An unmediated NO biosensor was prepared by co-immobilizing haemoglobin with polyethyleneimine at a pyrolytic graphite electrode surface. Haemoglobin exhibits direct electrochemistry in the membrane, and this modified electrode shows high stability and catalytic activity towards the reduction of NO. A linear relationship between the catalytic current and the concentration of NO was observed in the concentration ranges 1.0 x 10 -7 -8.0 x 10 -6 M and 1.0 x 10 -5 -2.5 x 10 -5 M, with a detection limit of 4.0 x 10 -8 M.

Published
2003

4. Nitric oxide biosensors based on Hb/phosphatidylcholine films

Author

Pingping Shen, Jiantao Pang, Chunhai Fan, Genxi Li, and Dexu Zhu

Subjects
Detection limit, Analytical chemistry, Electrons, Membranes, Artificial, Biosensing Techniques, Electrochemistry, Nitric Oxide, Analytical Chemistry, chemistry.chemical_compound, Hemoglobins, chemistry, Phosphatidylcholine, Electrode, Phosphatidylcholines, Animals, Reactivity (chemistry), Cattle, Pyrolytic carbon, Selectivity, Biosensor, Oxidation-Reduction, Algorithms, Nuclear chemistry
Abstract

Hemoglobin (Hb) was entrapped in a phosphatidylcholine (PC) film and immobilized at a pyrolytic graphite (PG) electrode surface. Its electron-transfer reactivity and enzyme activity were characterized by employing electrochemical methods. It was observed that Hb exhibited direct electrochemistry as well as enzyme-like activity towards the electrocatalytic reduction of NO in PC film. An unmediated, reagentless nitrogen oxide (NO) biosensor was accordingly prepared. Experimental results revealed that the peak current related to NO was linearly proportional to its concentration in the range of 1.0 x 10(-7)-3.0 x 10(-4) mol/L. The detection limit was estimated to be 1.0 x 10(-7) mol/L. Considering its good stability, nice selectivity and easy construction, this biosensor shows great promise for the rapid determination of traces of NO.

Published
2002

5. Weight Monitoring and Reporting Considerations of Liwan 3-1 Mega Jacket.

Author

Tui Deng, Baojie Chen, Zhengrong Song, Yi Lv, Shanhui Xu, Wenhao Zhang, and Jiantao Pang

Abstract

The article discusses research which provided an overview of the weight monitoring and reporting process of the single piece jacket during the detailed design and construction phase. Topics discussed include the Liwan3-1 (LW3-1) gas field in South China Sea consisting of deep water sub-sea developments and emphasis on the method to accurately generate weight data as part of the weight report process.

Published
2015

6. A reagentless nitric oxide biosensor based on haemoglobin/polyethyleneimine film.

Author

Xinjian Liu, Libin Shang, Jiantao Pang, and Genxi Li

Subjects
BIOSENSORS, HEMOGLOBINS, GRAPHITE, ELECTRODES, ELECTROCHEMISTRY, CELL membranes
Abstract

An unmediated NO biosensor was prepared by co-immobilizing haemoglobin with polyethyleneimine at a pyrolytic graphite electrode surface. Haemoglobin exhibits direct electrochemistry in the membrane, and this modified electrode shows high stability and catalytic activity towards the reduction of NO. A linear relationship between the catalytic current and the concentration of NO was observed in the concentration ranges 1.0×10-7–8.0×10-6 M and 1.0×10-5–2.5×10-5 M, with a detection limit of 4.0×10-8 M. [ABSTRACT FROM AUTHOR]

Published
2003

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