15 results on '"Jochen Kuckelkorn"'
Search Results
2. Chemical and in vitro bioanalytical assessment of drinking water quality in Manhiça, Mozambique
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Iro Evlampidou, Fernando Goñi-Irigoyen, Ariel Nhacolo, Lourdes Arjona, Arsenio Etxeandia, Valdemiro Escola, Beatriz Lazaro, Cristina M. Villanueva, Tamara Grummt, Jose Muñoz, Berta Grau-Pujol, Jochen Kuckelkorn, and Enrique Ulibarrena
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education.field_of_study ,Cadmium ,Haloacetic acids ,Epidemiology ,Population ,Public Health, Environmental and Occupational Health ,chemistry.chemical_element ,030501 epidemiology ,Toxicology ,medicine.disease_cause ,Pollution ,Ames test ,Mercury (element) ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry ,Environmental chemistry ,medicine ,Water quality ,0305 other medical science ,education ,Fluoride ,Genotoxicity ,medicine.drug - Abstract
Background The chemical quality of drinking water is widely unknown in low-income countries. Objective We conducted an exploratory study in Manhica district (Mozambique) to evaluate drinking water quality using chemical analyses and cell-based assays. Methods We measured nitrate, fluoride, metals, pesticides, disinfection by-products, and industrial organochlorinated chemicals, and conducted the bioassays Ames test for mutagenicity, micronuclei assay (MN-FACS), ER-CALUX, and antiAR-CALUX in 20 water samples from protected and unprotected sources. Results Nitrate was present in all samples (median 7.5 mg/L). Manganese, cobalt, chromium, aluminium, and barium were present in 90-100% of the samples, with median values of 32, 0.6, 2.0, 61, 250 μg/l, respectively. Manganese was above 50 μg/l (EU guideline) in eight samples. Arsenic, lead, nickel, iron, and selenium median values were below the quantification limit. Antimony, cadmium, copper, mercury, zinc and silver were not present. Trihalomethanes, haloacetic acids, haloacetonitriles and haloketones were present in 5-28% samples at levels ≤4.6 μg/l. DDT, dieldrin, diuron, and pirimiphos-methyl were quantified in 2, 3, 3, and 1 sample, respectively (range 12-60 ng/L). Fluoride was present in one sample (0.11 mg/l). Trichloroethene and tetrachloroethene were not present. Samples were negative in the in vitro assays. Significance Results suggest low exposure to chemicals, mutagenicity, genotoxicity and endocrine disruption through drinking water in Manhica population. High concentration of manganese in some samples warrants confirmatory studies, given the potential link to impaired neurodevelopment.
- Published
- 2021
3. Identification of Disinfection By-Products (Dbp) in Thermal Water Swimming Pools Applying Non-Target Screening by Lc-/Gc-Hrms
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Muhammad Usman, Jochen Kuckelkorn, Alexander Kämpfe, Christian Zwiener, Thomas A. Wintgens, and Volker Linnemann
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History ,Environmental Engineering ,Polymers and Plastics ,Health, Toxicology and Mutagenesis ,Environmental Chemistry ,Business and International Management ,Pollution ,Waste Management and Disposal ,Industrial and Manufacturing Engineering - Published
- 2022
4. Assessing the protection gap for mobile and persistent chemicals during advanced water treatment – A study in a drinking water production and wastewater treatment plant
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Grete Gollong, Isabelle J. Neuwald, Jochen Kuckelkorn, Ralf Junek, and Daniel Zahn
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Environmental Engineering ,Charcoal ,Drinking Water ,Ecological Modeling ,Wastewater ,Pollution ,Waste Management and Disposal ,Water Pollutants, Chemical ,Water Purification ,Water Science and Technology ,Civil and Structural Engineering - Abstract
Persistent and mobile (PM) chemicals spread quickly in the water cycle and can reach drinking water. If these chemicals are also toxic (PMT) they may pose a threat to the aquatic environment and drinking water alike, and thus measures to prevent their spread are necessary. In this study, nontarget screening and cell-based toxicity tests after a polarity-based fractionation into polar and non-polar chemicals are utilized to assess and compare the effectiveness of ozonation and filtration through activated carbon in a wastewater treatment and drinking water production plant. Especially during wastewater treatment, differences in removal efficiency were evident. While median areas of non-polar features were reduced by a factor of 270, median areas for polar chemicals were only reduced by a factor of 4. Polar features showed significantly higher areas than their non-polar counterparts in wastewater treatment plant effluent and finished drinking water, implying a protection gap for these chemicals. Toxicity tests revealed higher initial toxicities (especially oxidative stress and estrogenic activity) for the non-polar fraction, but also showed a more pronounced decrease during treatment. Generally, the toxicity of the effluent was low for both fractions. Combined, these results imply a less effective removal but also a lower toxicity of polar chemicals. The behaviour of features during advanced waste and drinking water treatment was used to classify them as either PM chemicals or mobile transformation products (M-TPs). A suspect screening of the 476 highest intensity PM chemicals and M-TPs in 57 environmental and tap water samples showed high frequencies of detection (median80%), which indicates the wide distribution of these chemicals in the aquatic environment and thus supports the chosen classification approach and the more generally applicability of obtained insights.
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- 2022
5. Cellular Uptake and Toxicological Effects of Differently Sized Zinc Oxide Nanoparticles in Intestinal Cells
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Glei, Anna Mittag, Christian Hoera, Alexander Kämpfe, Martin Westermann, Jochen Kuckelkorn, Thomas Schneider, and Michael
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apoptosis ,Caco-2 ,cell cycle ,cytotoxicity ,genotoxicity ,LT97 ,nanoparticles ,zinc oxide - Abstract
Due to their beneficial properties, the use of zinc oxide nanoparticles (ZnO NP) is constantly increasing, especially in consumer-related areas, such as food packaging and food additives, which is leading to an increased oral uptake of ZnO NP. Consequently, the aim of our study was to investigate the cellular uptake of two differently sized ZnO NP (
- Published
- 2021
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6. Chemical and in vitro bioanalytical assessment of drinking water quality in Manhiça, Mozambique
- Author
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Cristina M, Villanueva, Berta, Grau-Pujol, Iro, Evlampidou, Valdemiro, Escola, Fernando, Goñi-Irigoyen, Jochen, Kuckelkorn, Tamara, Grummt, Lourdes, Arjona, Beatriz, Lazaro, Arsenio, Etxeandia, Enrique, Ulibarrena, Ariel, Nhacolo, and Jose, Muñoz
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Drinking Water ,Water Quality ,Mozambique ,Water Pollutants, Chemical ,Arsenic ,Environmental Monitoring - Abstract
The chemical quality of drinking water is widely unknown in low-income countries.We conducted an exploratory study in Manhiça district (Mozambique) to evaluate drinking water quality using chemical analyses and cell-based assays.We measured nitrate, fluoride, metals, pesticides, disinfection by-products, and industrial organochlorinated chemicals, and conducted the bioassays Ames test for mutagenicity, micronuclei assay (MN-FACS), ER-CALUX, and antiAR-CALUX in 20 water samples from protected and unprotected sources.Nitrate was present in all samples (median 7.5 mg/L). Manganese, cobalt, chromium, aluminium, and barium were present in 90-100% of the samples, with median values of 32, 0.6, 2.0, 61, 250 μg/l, respectively. Manganese was above 50 μg/l (EU guideline) in eight samples. Arsenic, lead, nickel, iron, and selenium median values were below the quantification limit. Antimony, cadmium, copper, mercury, zinc and silver were not present. Trihalomethanes, haloacetic acids, haloacetonitriles and haloketones were present in 5-28% samples at levels ≤4.6 μg/l. DDT, dieldrin, diuron, and pirimiphos-methyl were quantified in 2, 3, 3, and 1 sample, respectively (range 12-60 ng/L). Fluoride was present in one sample (0.11 mg/l). Trichloroethene and tetrachloroethene were not present. Samples were negative in the in vitro assays.Results suggest low exposure to chemicals, mutagenicity, genotoxicity and endocrine disruption through drinking water in Manhiça population. High concentration of manganese in some samples warrants confirmatory studies, given the potential link to impaired neurodevelopment.
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- 2020
7. Filling the knowledge gap: A suspect screening study for 1310 potentially persistent and mobile chemicals with SFC- and HILIC-HRMS in two German river systems
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Urs Berger, Thorsten Reemtsma, Claudia Strobel, Thomas P. Knepper, Matthias Muschket, Till Meier, Isabelle J. Neuwald, Jochen Kuckelkorn, Daniel Zahn, and Bettina Seiwert
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Prioritization ,Environmental Engineering ,Chromatography ,business.industry ,Ecological Modeling ,Hydrophilic interaction chromatography ,Chromatography, Supercritical Fluid ,Chemical industry ,Pollution ,Mass Spectrometry ,chemistry.chemical_compound ,Rivers ,chemistry ,Liquid chromatography–mass spectrometry ,Ionic liquid ,Supercritical fluid chromatography ,business ,Hydrophobic and Hydrophilic Interactions ,Waste Management and Disposal ,Surface water ,Screening study ,Chromatography, Liquid ,Water Science and Technology ,Civil and Structural Engineering - Abstract
Persistent and mobile chemicals (PM chemicals) were searched for in surface waters by hydrophilic interaction liquid chromatography (HILIC) and supercritical fluid chromatography (SFC), both coupled to high resolution mass spectrometry (HRMS). A suspect screening was performed using a newly compiled list of 1310 potential PM chemicals to the data of 11 surface water samples from two river systems. In total, 64 compounds were identified by this approach. The overlap between HILIC- and SFC-HRMS was limited (31 compounds), confirming the complementarity of the two methods used. The identified PM candidates are characterized by a high polarity (median logD -0.4 at pH 7.5), a low molecular weight (median 187 g/mol), are mostly ionic (54 compounds) and contain a large number of heteroatoms (one per four carbons on average). Among the most frequently detected novel or yet scarcely investigated water contaminants were cyanoguanidine (11/11 samples), adamantan-1-amine (10/11), trifluoromethanesulfonate (9/11), 2-acrylamido-2-methylpropanesulfonate (10/11), and the inorganic anions hexafluorophosphate (11/11) and tetrafluoroborate (10/11). 31% of the identified suspects are mainly used in ionic liquids, a chemically diverse group of industrial chemicals with numerous applications that is so far rarely studied for their occurrence in the environment. Prioritization of the findings of PM candidates is hampered by the apparent lack of toxicity data. Hence, precautionary principles and minimization approaches should be applied for the risk assessment and risk management of these substances. The large share of novel water contaminants among these findings of the suspect screening indicates that the universe of PM chemicals present in the environment has so far only scarcely been explored. Dedicated analytical methods and screening lists appear essential to close the analytical gap for PM compounds.
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- 2021
8. A hierarchical testing strategy for micropollutants in drinking water regarding their potential endocrine-disrupting effects—towards health-related indicator values
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Thomas-Benjamin Seiler, Petra Waldmann, Regine Redelstein, Tamara Grummt, Henner Hollert, Timon Heide, Sibylle Maletz, Jennifer Kunze, and Jochen Kuckelkorn
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0301 basic medicine ,Health, Toxicology and Mutagenesis ,Endocrine Disruptors ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Tributyltin oxide ,Germany ,medicine ,Humans ,Environmental Chemistry ,Ecotoxicology ,Bioassay ,Endocrine system ,Atrazine ,0105 earth and related environmental sciences ,Chemistry ,Drinking Water ,General Medicine ,Pesticide ,Pollution ,030104 developmental biology ,Environmental chemistry ,Biological Assay ,Water quality ,Water Pollutants, Chemical ,Genotoxicity ,Environmental Monitoring - Abstract
In Germany, micropollutants that (may) occur in drinking water are assessed by means of the health-related indicator value (HRIV concept), developed by the German Federal Environment Agency. This concept offers five threshold values (≤ 0.01 to ≤ 3 μg l−1) depending on availability and completeness of data regarding genotoxicity, neurotoxicity, and germ cell-damaging potential. However, the HRIV concept is yet lacking integration of endocrine disruptors as one of the most prominent toxicological concerns in water bodies, including drinking water. Thresholds and proposed bioassays hence urgently need to be defined. Since endocrine disruption of ubiquitary chemicals as pharmaceuticals, industrial by-products, or pesticides is a big issue in current ecotoxicology, the aim of this study was to explore endocrine effects, i.e., estrogenic and androgenic effects, as an important, additional toxicological mode of action for the HRIV concept using a hierarchical set of well-known but improved bioassays. Results indicate that all of the 13 tested substances, industrial chemicals and combustion products (5), pharmaceuticals and medical agents (4), and pesticides and metabolites (4), have no affinity to the estrogen and androgen receptor in human U2OS cells without metabolic activation, even when dosed at their water solubility limit, while in contrast some of these substances showed estrogenic effects in the RYES assay, as predicted in pre-test QSAR analysis. Using a specifically developed S9-mix with the U2OS cells, those micropollutants, i.e., Benzo[a]pyrene, 2,4-Dichlorophenol, 3,3-Dichlorbenzidin, 3,4-Dichloranilin, and diclofenac, they show estrogenic effects at the same concentration range as for the yeast cells. Three of the drinking water-relevant chemicals, i.e., atrazine, tributyltin oxide, and diclofenac, caused effects on hormone production in the H295R assay, which can be correlated with changes in the expression of steroidogenic genes. One chemical, 17α-Ethinylestradiol, caused an estrogenic or anti-androgenic effect in the reproduction test with Potamopyrgus antipodarum. Considering these results, a proposal for a test strategy for micropollutants in drinking water regarding potential endocrine effects (hormonal effects on reproduction and sexual development) will be presented to enhance the existing HRIV concept.
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- 2017
9. The metabolite 3,4,3ʹ,4ʹ-tetrachloroazobenzene (TCAB) exerts a higher ecotoxicity than the parent compounds 3,4-dichloroaniline (3,4-DCA) and propanil
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Hongxia Xiao, Jochen Kuckelkorn, Henner Hollert, Andreas Schäffer, Michael Patrick Hennig, Tilman Floehr, Martina Roß-Nickoll, and Leonie Nüßer
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0301 basic medicine ,Environmental Engineering ,Metabolite ,Propanil ,010501 environmental sciences ,Biology ,Pharmacology ,Chlorobenzenes ,Ecotoxicology ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Endocrine disrupting compound ,Toxicity Tests ,Cytochrome P-450 CYP1A1 ,Environmental Chemistry ,CALUX ,Bioassay ,Waste Management and Disposal ,Toxic equivalency factor ,0105 earth and related environmental sciences ,Aniline Compounds ,Herbicides ,Aryl hydrocarbon receptor ,Pollution ,030104 developmental biology ,Receptors, Aryl Hydrocarbon ,chemistry ,Toxicity ,biology.protein ,Environmental Pollutants ,Azo Compounds - Abstract
3,4,3ʹ,4ʹ-tetrachloroazobenzene (TCAB) is not commercially manufactured but formed as an unwanted by-product in the manufacturing of 3,4-dichloroaniline (3,4-DCA) or metabolized from the degradation of chloranilide herbicides, like propanil. While a considerable amount of research has been done concerning the toxicological and ecotoxicological effects of propanil and 3,4-DCA, limited information is available on TCAB. Our study examined the toxicity of TCAB in comparison to its parent compounds propanil and 3,4-DCA, using a battery of bioassays including in vitro with aryl hydrocarbon receptor (AhR) mediated activity by the 7-ethoxyresorufin- O -deethylase (EROD) assay and micro-EROD, endocrine-disrupting activity with chemically activated luciferase gene expression (CALUX) as well as in vivo with fish embryo toxicity (FET) assays with Danio rerio . Moreover, the quantitative structure activity response (QSAR) concepts were applied to simulate the binding affinity of TCAB to certain human receptors. It was shown that TCAB has a strong binding affinity to the AhR in EROD and micro-EROD induction assay, with the toxic equivalency factor (TEF) of 8.7 × 10 − 4 and 1.2 × 10 − 5 , respectively. TCAB presented to be a weak endocrine disrupting compound with a value of estradiol equivalence factor (EEF) of 6.4 × 10 − 9 and dihydrotestosterone equivalency factor (DEF) of 1.1 × 10 − 10 . No acute lethal effects of TCAB were discovered in FET test after 96 h of exposure. Major sub-lethal effects detected were heart oedema, yolk malformation, as well as absence of blood flow and tail deformation. QSAR modelling suggested an elevated risk to environment, particularly with respect to binding to the AhR. An adverse effect potentially triggering ERβ, mineralocorticoid, glucocorticoid and progesterone receptor activities might be expected. Altogether, the results obtained suggest that TCAB exerts a higher toxicity than both propanil and 3,4-DCA. This should be considered when assessing the impact of these compounds for the environment and also for regulatory decisions.
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- 2016
10. Estrogenic activity in Finnish municipal wastewater effluents
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Henner Hollert, Noora Perkola, Pia Välitalo, Markus Sillanpää, Thomas-Benjamin Seiler, Jochen Kuckelkorn, Eija Schultz, and Anna Mikola
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010504 meteorology & atmospheric sciences ,Estrogenic activity ,TREATMENT-PLANT EFFLUENTS ,Wastewater ,010501 environmental sciences ,Waste Disposal, Fluid ,01 natural sciences ,REPORTER GENE ASSAY ,chemistry.chemical_compound ,Tandem Mass Spectrometry ,Liquid chromatography–mass spectrometry ,SEWAGE-TREATMENT PLANTS ,Bioassay ,Chemical analysis ,Solid phase extraction ,ta519 ,Waste Management and Disposal ,ta116 ,ta512 ,Finland ,Water Science and Technology ,IN-VITRO BIOASSAYS ,ta212 ,Reproduction ,Ecological Modeling ,Aliivibrio fischeri ,Pollution ,SOLID-PHASE EXTRACTION ,Environmental chemistry ,Sewage treatment ,hormones, hormone substitutes, and hormone antagonists ,Environmental Engineering ,ta1172 ,Enzyme-Linked Immunosorbent Assay ,Estrone ,In vitro ,ENDOCRINE DISRUPTING COMPOUNDS ,Toxicity Tests ,Animals ,TANDEM MASS-SPECTROMETRY ,Effluent ,ta218 ,0105 earth and related environmental sciences ,Civil and Structural Engineering ,Detection limit ,Estrogens ,ENVIRONMENTAL RISK-ASSESSMENT ,PERFORMANCE LIQUID-CHROMATOGRAPHY ,Daphnia ,chemistry ,MULTIPLE HORMONAL ACTIVITIES ,Water Pollutants, Chemical ,Wastewater effluent ,Chromatography, Liquid - Abstract
Effluents from wastewater treatment plants (WWTPs) are a major source of estrogenic compounds to the aquatic environment. In the present work, estrogenic activities of effluents from eight municipal WWTPs in Finland were studied. The main objectives of the study were to quantify the concentrations of selected estrogenic compounds, to evaluate their contribution to estrogenic potency and to test the feasibility of the commercial bioassays for wastewater analysis. The effluent samples were analyzed by two in vitro tests, i.e. ERα-CALUX(®) and ELISA-E2, and by liquid chromatography mass spectrometry for six estrogenic compounds: estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), 17α-estradiol and bisphenol A (BPA). Estrogenic effects were found in all of the effluent samples with both of the bioassays. The concentrations measured with ELISA-E2 (8.6-61.6 ng/L) were clearly higher but exhibited a similar pattern than those with chemical analysis (E2
- Published
- 2016
11. Pilot study on chemical pollution of drinking water sources in rural Mozambique
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Goñi F, Grau B, Lourdes Arjona, Jochen Kuckelkorn, Cristina M. Villanueva, Jose Muñoz, and Iro Evlampidou
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Global and Planetary Change ,Epidemiology ,Health, Toxicology and Mutagenesis ,Water source ,Public Health, Environmental and Occupational Health ,Environmental science ,Chemical pollution ,Water resource management ,Pollution - Published
- 2019
12. How flood events affect rainbow trout: Evidence of a biomarker cascade in rainbow trout after exposure to PAH contaminated sediment suspensions
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John P. Giesy, Henner Hollert, Michelle Chinoraks, Jan Wölz, Andreas Schäffer, Catrina Cofalla, Holger Schüttrumpf, Markus Brinkmann, Steve Wiseman, Jochen Kuckelkorn, Ulrike Kammann, Markus Hecker, Michael Patrick Hennig, and Sebastian Hudjetz
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Chrysene ,Geologic Sediments ,Ecology ,Health, Toxicology and Mutagenesis ,Metabolite ,Temperature ,Sediment ,Biota ,Context (language use) ,Environmental Exposure ,Environmental exposure ,Aquatic Science ,Survival Analysis ,Floods ,chemistry.chemical_compound ,chemistry ,Oncorhynchus mykiss ,Environmental chemistry ,Animals ,Pyrene ,Rainbow trout ,Polycyclic Aromatic Hydrocarbons ,Biomarkers ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Increasing frequency and intensity of flood events are major concerns in the context of climate change. In addition to the direct hydrological implications of such events, potential ecotoxicological impacts are of increasing interest. It is vital to understand mechanisms of contaminant uptake from suspended particulate matter (SPM) and related effects in aquatic biota under realistic conditions. However, little is known about these processes. Due to recent changes in climate, during summer temperatures of German rivers frequently exceed 25°C. Effects of re-suspension of sediments on biota under elevated temperature regimes are likely to differ from those under lower temperature regimes. To elucidate this differential response of aquatic vertebrates, rainbow trout were exposed to suspensions of sediment from the Rhine River that was spiked with a mixture of polycyclic aromatic hydrocarbons (PAH). The experiments were conducted under two different temperature regimes (24°C or 12°C). Physicochemical parameters, including concentration of PAHs in SPM, and biomarkers in fish (biliary PAH metabolites, 7-ethoxyresorufin O-deethylase activity, lipid peroxidation (LPO), mRNA expression of some genes and micronuclei) were measured over the course of a 12d study. Concentrations of pyrene and phenanthrene decreased over time, while no decrease was observed for chrysene and benzo[a]pyrene. The biomarker cascades, more specifically the temporal dynamics of biomarker reactions, did not only show quantitative differences (i.e. different induction intensity or rate of biomarker responses) at the two temperatures but also qualitative differences, i.e. different biomarker responses were observed. A slight significant increase of biliary metabolites in fish was observed in un-spiked sediment at 24°C. In bile of fish exposed to PAH spiked sediment concentrations of 1-hydroxypyrene and 1-hydroxyphenanthrene increased significantly during the first two days, and then decreased. At 12°C uptake of PAHs was slower and maximum metabolite concentrations in bile were less than in fish exposed at 24°C. Following a latency of two days, concentrations of PAH metabolites in bile of fish exposed at 24°C were followed by a peak in LPO. PAHs spiked into sediments under laboratory conditions were significantly more bioavailable than the PAHs that were already present in un-spiked field-collected sediments.
- Published
- 2013
13. Heterocyclic aromatic hydrocarbons show estrogenic activity upon metabolization in a recombinant transactivation assay
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Martin Krauss, Henner Hollert, Kerstin Bluhm, Sibylle Maletz, Jochen Kuckelkorn, Sabrina Schiwy, Markus Brinkmann, Werner Brack, and Andreas Tiehm
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Transcriptional Activation ,Bisphenol A ,Quantitative Structure-Activity Relationship ,chemistry.chemical_compound ,Tandem Mass Spectrometry ,Cell Line, Tumor ,Confidence Intervals ,Environmental Chemistry ,Bioassay ,Organic chemistry ,Humans ,Polycyclic Aromatic Hydrocarbons ,Xanthene ,Indole test ,Recombination, Genetic ,Quinoline ,Water ,Estrogens ,General Chemistry ,Dibenzofuran ,chemistry ,Receptors, Estrogen ,Dibenzothiophene ,Acridine ,Biological Assay ,Chromatography, Liquid - Abstract
Heterocyclic aromatic hydrocarbons (hetero-PAHs) are increasingly studied at contaminated sites; especially at former industrial facilities where coal tar-oil was handled, e.g., wood treatment plants, high concentrations of hetero-PAHs are frequently detected in groundwater plumes. In previous studies, fractions of groundwater with high estrogenic activity contained hetero-PAHs and their hydroxylated metabolites. To evaluate this preliminary evidence, selected hetero-PAHs were screened for their estrogenic activity in lyticase yeast estrogen screen (LYES) and ER CALUX. All tested substances were inactive in the LYES. Hetero-PAHs such as acridine, xanthene, indole, 2-methylbenzofuran, 2,3-dimethylbenzofuran, dibenzofuran, dibenzothiophene, quinoline, and 6-methylquinoline were positive in the ER CALUX, with estradiol equivalence factors (EEFs) from 2.85 × 10(-7) to 3.18 × 10(-5). The EEF values of these substances were comparable to those of other xenoestrogens (e.g., alkylphenols or bisphenol A) that are sometimes found in surface water. Chemical analyses revealed that T47Dluc cells could metabolize most of the substances. Among the metabolites (tentatively) identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were hydroxides and their keto tautomers, sulfates, sulfoxides, and N-oxides. Because of their high concentrations measured in groundwater, we conclude that hetero-PAHs and metabolites may be a potential risk and should be the subject of further research.
- Published
- 2014
14. Application of in-situ bioassays with macrophytes in aquatic mesocosm studies
- Author
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Monika Hammers-Wirtz, Anja Coors, Jochen Kuckelkorn, and Tido Strauss
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Myriophyllum ,biology ,Ecology ,Triazines ,Health, Toxicology and Mutagenesis ,Lake ecosystem ,General Medicine ,Terbuthylazine ,Management, Monitoring, Policy and Law ,Toxicology ,biology.organism_classification ,Zooplankton ,Macrophyte ,Mesocosm ,chemistry.chemical_compound ,Potamogeton lucens ,chemistry ,Aquatic plant ,Environmental science ,Ecotoxicology ,Animals ,Biological Assay ,Ecosystem ,Water Pollutants, Chemical - Abstract
Aquatic mesocosm studies assess ecotoxicological effects of chemicals by using small artificial ponds as models of lentic ecosystems. In this study, methods of controlled insertion of macrophytes within an outdoor mesocosm study were explored. Although analytically confirmed concentrations of the model herbicide terbuthylazine were high enough to expect direct effects on phytoplankton, functional parameters and dominant taxa abundance indicated only minor and transient effects. In-situ assays with Lemna minor, Myriophyllum spicatum, Potamogeton lucens and Chara globularis revealed adverse effects at concentrations in accordance with literature data. Complex interactions such as nutrient limitation and competition were possible reasons for the observed growth promotion at the lower concentration of about 5 microg/l terbuthylazine. The approach of macrophyte in-situ bioassays within a mesocosm study proved to be applicable. Presumed advantages are simultaneous acquisition of toxicity data for several species of aquatic plants under more realistic conditions compared to laboratory tests and inclusion of macrophytes as important structural and functional components in mesocosms while limiting their domination of the model ecosystem.
- Published
- 2006
15. Tox-Box: Securing drops of life - An enhanced health-related approach for risk assessment of drinking water in Germany
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Sibylle Maletz, Henner Hollert, Petra Waldmann, Elke Rabbow, Birgit Kneib-Kissinger, Rita Heinze, Martina Knauer, Tamara Grummt, Tobias Schulze, Thomas Braunbeck, Alexander Eckhardt, Arnold Bahlmann, Walter Meinl, Hansruedi Glatt, Jochen Kuckelkorn, Walter Schadenboeck, Sebastian Feles, Christine E. Hellweg, Werner Brack, Thomas-Benjamin Seiler, Daniel Stengel, Carsten K. Schmidt, Stefan Gartiser, Martin Krauss, Ralf Junek, Petra Rettberg, Eberhard Küster, Abu Noman, Luis F. Spitta, Christa Baumstark-Khan, Meike Kramer, Regine Redelstein, and Eva-Maria Prantl
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Test strategy ,business.industry ,Water supply ,Health related ,Context (language use) ,Pollution ,Task (project management) ,Toxicology ,Environmental science ,Indicator value ,business ,Risk assessment ,Environmental planning ,Risk management - Abstract
This article introduces ‘Tox-Box’, a joint research project designed to develop a holistic approach towards a harmonized testing strategy for exposure- and hazard-based risk management of anthropogenic trace substances in drinking water to secure a long-term drinking water supply. The main task of the Tox-Box consortium is to enhance the existing health-related indicator value concept (German: GOW-Konzept - Gesundheitlicher Orientierungswert) through development and prioritization of additional end point-related testing strategies for genotoxicity, neurotoxicity, germ cell damage, and endocrine effects. In this context, substance-specific modes of action will be identified and characterized. Toxicological data collected by the 12 Tox-Box subprojects will be evaluated and weighted to structure a hierarchical testing strategy for an improved risk assessment. A technical guidance document for exposure and hazard-based risk management of anthropogenic trace substances in drinking water will eventually be prepared.
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