Search

Your search keyword '"Kinda Schepers"' showing total 27 results
Start Over Author "Kinda Schepers"
27 results on '"Kinda Schepers"'

1. Reassessment of the risk of birth defects due to Zika virus in Guadeloupe, 2016.

Author

Anna L Funk, Bruno Hoen, Ingrid Vingdassalom, Catherine Ryan, Philippe Kadhel, Kinda Schepers, Stanie Gaete, Benoit Tressières, and Arnaud Fontanet

Subjects
Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270
Abstract

BackgroundIn the French Territories in the Americas (FTA), the risk of birth defects possibly associated with Zika virus (ZIKV) infection was 7.0% (95%CI: 5.0 to 9.5) among foetuses/infants of 546 women with symptomatic RT-PCR confirmed ZIKV infection during pregnancy. Many of these defects were isolated measurement-based microcephaly (i.e. without any detected brain or clinical abnormalities) or mild neurological conditions. We wanted to estimate the proportion of such minor findings among live births of women who were pregnant in the same region during the outbreak period but who were not infected with ZIKV.MethodsIn Guadeloupe, pregnant women were recruited at the time of delivery and tested for ZIKV infection. The outcomes of live born infants of ZIKV non-infected women were compared to those of ZIKV-exposed live born infants in Guadeloupe, extracted from the FTA prospective cohort.ResultsOf 490 live born infants without exposure to ZIKV, 42 infants (8.6%, 95%CI: 6.2-11.4) had mild abnormalities that have been described as 'potentially linked to ZIKV infection'; all but one of these was isolated measurement-based microcephaly. Among the 241 live born infants with ZIKV exposure, the proportion of such abnormalities, using the same definition, was similar (6.6%, 95%CI: 3.8-10.6).ConclusionsIsolated anthropometric abnormalities and mild neurological conditions were as prevalent among infants with and without in-utero ZIKV exposure. If such abnormalities had not been considered as 'potentially linked to ZIKV' in the original prospective cohort in Guadeloupe, the overall estimate of the risk of birth defects considered due to the virus would have been significantly lower, at approximately 1.6% (95% CI: 0.4-4.1).Trial registrationClinicalTrials.gov (NCT02916732).

Published
2021
Full Text
View/download PDF

2. Severe Sepsis and Septic Shock Associated with Chikungunya Virus Infection, Guadeloupe, 2014

Author

Amélie Rollé, Kinda Schepers, Sylvie Cassadou, Elodie Curlier, Benjamin Madeux, Cécile Hermann-Storck, Isabelle Fabre, Isabelle Lamaury, Benoit Tressières, Guillaume Thiery, and Bruno Hoen

Subjects
chikungunya, severe sepsis, septic shock, CHIKV, vector-borne infections, viruses, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

During a 2014 outbreak, 450 patients with confirmed chikungunya virus infection were admitted to the University Hospital of Pointe-à-Pitre, Guadeloupe. Of these, 110 were nonpregnant adults; 42 had severe disease, and of those, 25 had severe sepsis or septic shock and 12 died. Severe sepsis may be a rare complication of chikungunya virus infection.

Published
2016
Full Text
View/download PDF

3. Severe Thrombocytopenia after Zika Virus Infection, Guadeloupe, 2016

Author

Timothée Boyer Chammard, Kinda Schepers, Sébastien Breurec, Thierry Messiaen, Anne-Laure Destrem, Matthieu Mahevas, Adrien Soulillou, Ludovic Janaud, Elodie Curlier, Cécile Herrmann-Storck, and Bruno Hoen

Subjects
Zika virus, viruses, infection, thrombocytopenia, hemorrhagic symptoms, Guadeloupe, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Severe thrombocytopenia during or after the course of Zika virus infection has been rarely reported. We report 7 cases of severe thrombocytopenia and hemorrhagic signs and symptoms in Guadeloupe after infection with this virus. Clinical course and laboratory findings strongly suggest a causal link between Zika virus infection and immune-mediated thrombocytopenia.

Published
2017
Full Text
View/download PDF

4. Liver Abscess Caused by Infection with Community-Acquired Klebsiella quasipneumoniae subsp. quasipneumoniae

Author

Sebastien Breurec, Benedicte Melot, Bruno Hoen, Virginie Passet, Kinda Schepers, Sylvaine Bastian, and Sylvain Brisse

Subjects
Klebsiella quasipneumoniae subsp. quasipneumoniae, liver abscess, integrative and conjugative element ICEKp1, capsular serotype K1, bacteria, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

We report a case of pyogenic liver abscess caused by community-acquired Klebsiella quasipneumoniae subsp. quasipneumoniae. The infecting isolate had 2 prominent features of hypervirulent K. pneumoniae strains: the capsular polysaccharide synthesis region for K1 serotype and the integrative and conjugative element ICEKp1, which encodes the virulence factors yersiniabactin, salmochelin, and RmpA.

Published
2016
Full Text
View/download PDF

5. Risk stratification of latent tuberculosis defined by combined interferon gamma release assays.

Author

Véronique Corbière, Gaelle Pottier, Florence Bonkain, Kinda Schepers, Virginie Verscheure, Sophie Lecher, T Mark Doherty, Camille Locht, and Françoise Mascart

Subjects
Medicine, Science
Abstract

BACKGROUND: Most individuals infected with Mycobacterium tuberculosis develop latent tuberculosis infection (LTBI). Some may progress to active disease and would benefit from preventive treatment yet no means currently exists to predict who will reactivate. Here, we provide an approach to stratify LTBI based on IFN-γ responses to two antigens, the recombinant Early-Secreted Antigen Target-6 (rESAT-6) and the latency antigen Heparin-Binding Haemagglutinin (HBHA). METHODS: We retrospectively analyzed results from in-house IFN-γ-release assays with HBHA (HBHA-IGRA) and rESAT-6 (rESAT-6-IGRA) performed during a 12-year period on serial blood samples (3 to 9) collected from 23 LTBI subjects in a low-TB incidence country. Both the kinetics of the absolute IFN-γ concentrations secreted in response to each antigen and the dynamics of HBHA/rESAT-6-induced IFN-γ concentrations ratios were examined. RESULTS: This analysis allowed the identification among the LTBI subjects of three major groups. Group A featured stable HBHA and rESAT-6-IGRA profiles with an HBHA/rESAT-6 ratio persistently higher than 1, and with high HBHA- and usually negative rESAT-6-IGRA responses throughout the study. Group B had changing HBHA/rESAT-6 ratios fluctuating from 0.0001 to 10,000, with both HBHA and rESAT-6 responses varying over time at least once during the follow-up. Group C was characterized by a progressive disappearance of all responses. CONCLUSIONS: By combining the measures of IFN-γ concentrations secreted in response to an early and a latency antigens, LTBI subjects can be stratified into different risk groups. We propose that disappearing responses indicate cure, that persistent responses to HBHA with HBHA/rESAT-6 ratios ≥ 1 represent stable LTBI subjects, whereas subjects with ratios varying from >1 to

Published
2012
Full Text
View/download PDF

6. Heparin-binding-hemagglutinin-induced IFN-gamma release as a diagnostic tool for latent tuberculosis.

Author

Jean-Michel Hougardy, Kinda Schepers, Sammy Place, Annie Drowart, Véronique Lechevin, Virginie Verscheure, Anne-Sophie Debrie, T Mark Doherty, Jean-Paul Van Vooren, Camille Locht, and Françoise Mascart

Subjects
Medicine, Science
Abstract

BACKGROUND: The detection of latent tuberculosis infection (LTBI) is a major component of tuberculosis (TB) control strategies. In addition to the tuberculosis skin test (TST), novel blood tests, based on in vitro release of IFN-gamma in response to Mycobacterium tuberculosis-specific antigens ESAT-6 and CFP-10 (IGRAs), are used for TB diagnosis. However, neither IGRAs nor the TST can separate acute TB from LTBI, and there is concern that responses in IGRAs may decline with time after infection. We have therefore evaluated the potential of the novel antigen heparin-binding hemagglutinin (HBHA) for in vitro detection of LTBI. METHODOLOGY AND PRINCIPAL FINDINGS: HBHA was compared to purified protein derivative (PPD) and ESAT-6 in IGRAs on lymphocytes drawn from 205 individuals living in Belgium, a country with low TB prevalence, where BCG vaccination is not routinely used. Among these subjects, 89 had active TB, 65 had LTBI, based on well-standardized TST reactions and 51 were negative controls. HBHA was significantly more sensitive than ESAT-6 and more specific than PPD for the detection of LTBI. PPD-based tests yielded 90.00% sensitivity and 70.00% specificity for the detection of LTBI, whereas the sensitivity and specificity for the ESAT-6-based tests were 40.74% and 90.91%, and those for the HBHA-based tests were 92.06% and 93.88%, respectively. The QuantiFERON-TB Gold In-Tube (QFT-IT) test applied on 20 LTBI subjects yielded 50% sensitivity. The HBHA IGRA was not influenced by prior BCG vaccination, and, in contrast to the QFT-IT test, remote (>2 years) infections were detected as well as recent (

Published
2007
Full Text
View/download PDF

7. Added Value of Long-Term Cytokine Release Assays to Detect Mycobacterium tuberculosis Infection in HIV-Infected Subjects in Uganda

Author

Mahavir Singh, Camille Locht, Luc Kestens, Françoise Mascart, William Worodria, Marguerite Massinga-Loembe, Kinda Schepers, Robert Colebunders, Violette Dirix, and TB-IRIS Study Grp

Subjects
0301 basic medicine, CD4-Positive T-Lymphocytes, medicine.medical_treatment, Human immunodeficiency virus (HIV), HIV Infections, interferon-gamma release assay, medicine.disease_cause, complex mixtures, Mycobacterium tuberculosis, 03 medical and health sciences, 0302 clinical medicine, Latent Tuberculosis, Hiv infected, medicine, Added value, Humans, Pharmacology (medical), Uganda, 030212 general & internal medicine, human, biology, human immunodeficiency virus, business.industry, Epidemiology and Prevention, respiratory system, bacterial infections and mycoses, biology.organism_classification, cytokines/chemokines, Predictive value, 030104 developmental biology, Infectious Diseases, Cytokine, Immunology, Cohort, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, Cytokines, Human medicine, business, tuberculosis-associated immune reconstitution inflammatory syndrome, Interferon-gamma Release Tests
Abstract

Supplemental Digital Content is Available in the Text., Objectives: To investigate whether mycobacterial antigen–induced cytokine secretions are helpful in detecting Mycobacterium tuberculosis (Mtb) infection in a cohort of HIV-infected patients living in a country with a high burden of Mtb and HIV infections, and to determine their predictive value for the development of tuberculosis (TB)-associated immune reconstitution inflammatory syndrome. Design: A total of 352 HIV-infected patients (186 with active TB) were prospectively enrolled when initiating antiretroviral therapy (ART). Sequential blood samples were collected during the first 6 months of ART. Eighty-three HIV-uninfected subjects (39 with active TB) were enrolled as controls. Methods: The concentrations of 13 cytokines were measured in supernatants from blood mononuclear cells in vitro stimulated with purified protein derivative (PPD), heparin-binding hemagglutinin (HBHA) or early secreted antigen-6 (ESAT-6) and culture filtrate protein-10 (CFP-10), and results were compared with those of tuberculin skin tests (TST). Results: The best detection of Mtb infection was achieved by ESAT-6/CFP-10–induced interferon-γ concentrations, but results were often negative for patients with CD4+ T-cell counts

Published
2016

8. Severe Sepsis and Septic Shock Associated with Chikungunya Virus Infection, Guadeloupe, 2014

Author

Elodie Curlier, Isabelle Lamaury, Cécile Hermann-Storck, Kinda Schepers, Amélie Rollé, Bruno Hoen, Benjamin Madeux, Guillaume Thiery, Sylvie Cassadou, Benoit Tressières, and Isabelle Fabre

Subjects
Male, chikungunya, Epidemiology, viruses, CHIKV, vector-borne infections, lcsh:Medicine, Comorbidity, medicine.disease_cause, Disease Outbreaks, 0302 clinical medicine, Pregnancy, French West Indies, Medicine, 030212 general & internal medicine, Chikungunya, Child, Guadeloupe, Aged, 80 and over, musculoskeletal, neural, and ocular physiology, Dispatch, virus diseases, Middle Aged, Shock, Septic, Infectious Diseases, Child, Preschool, Female, Chikungunya virus, Adult, Microbiology (medical), Adolescent, 030231 tropical medicine, macromolecular substances, lcsh:Infectious and parasitic diseases, Sepsis, Young Adult, 03 medical and health sciences, Humans, lcsh:RC109-216, Severe sepsis, Aged, Severe Sepsis and Septic Shock Associated with Chikungunya Virus Infection, Guadeloupe, 2014, business.industry, Septic shock, lcsh:R, Outbreak, medicine.disease, Virology, severe sepsis, Patient Outcome Assessment, nervous system, Chikungunya Fever, septic shock, business, Complication
Abstract

During a 2014 outbreak, 450 patients with confirmed chikungunya virus infection were admitted to the University Hospital of Pointe-à-Pitre, Guadeloupe. Of these, 110 were nonpregnant adults; 42 had severe disease, and of those, 25 had severe sepsis or septic shock and 12 died. Severe sepsis may be a rare complication of chikungunya virus infection.

Published
2016

9. Pregnancy Outcomes after ZIKV Infection in French Territories in the Americas

Author

Yoann Madec, Cécile Herrmann-Storck, Arnaud Fontanet, Bruno Hoen, Caroline Callier, Raymond Césaire, Dominique Rousset, Alice Monthieux, Cédric Laouénan, Manon Boullard, Anna L. Funk, Maylis Douine, S. Stegmann-Planchard, Benoit Tressières, Bruno Schaub, Fatiha Najioullah, Jean-Luc Voluménie, Eustase Janky, André Cabié, Gabriel Carles, Kinda Schepers, Samson Yassinguezo, Sylvie Cassadou, Vanessa Ardillon, Philippe Kadhel, Mathieu Nacher, Catherine Ryan, CHU Pointe-à-Pitre/Abymes [Guadeloupe], Centre d'investigation clinique Antilles-Guyane (CIC - Antilles Guyane), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de la Martinique [Fort de France]-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Université des Antilles et de la Guyane (UAG), Maison de la Femme de la Mère et de l'Enfant [CHU de la Martinique] (MFME [Fort de France]), CHU de la Martinique [Fort de France], Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Santé publique France - French National Public Health Agency [Saint-Maurice, France], Centre Hospitalier de l'Ouest Guyanais Franck Joly [Saint-Laurent-du-Maroni, Guyane Française], Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université des Antilles et de la Guyane - UFR des sciences médicales (UAG UFR SM), Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP), Maladies infectieuses et tropicales dans la Caraïbe (MAITC EA 4537), CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de la Martinique [Fort de France]-Université des Antilles (UA), Centre Hospitalier Louis Constant Fleming, Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Center for Global Health Research and Education - Centre pour la Recherche et la Formation en Santé Mondiale (CGH), Institut Pasteur [Paris] (IP)-Réseau International des Instituts Pasteur (RIIP), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Supported by the French Ministry of Health (Soutien Exceptionnel à la Recherche et à l’Innovation), by a grant (ANR-10-LABX-62-IBEID) from Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases project, by the ZIKAlliance consortium (the European Union’s Horizon 2020 Research and Innovation Program), and by INSERM. This study is part of the ZIKAlliance consortium project., We thank the women who participated in this study, all the physicians, midwives, clinical research assistants, health officers, and epidemiologists who helped conduct this study in each of the French territories in the Americas: Myriam Atine, Lyderic Aubert, Marie Barrau, Kebe Beavogui, Christelle Celeste, Joelle Colat-Peyron, Elvire Couchy, Véronique Davidas, Lucetta Delver, Jacqueline Dubois, Stanie Gaete, Dorothée Harrois, Marie-Laure Lalanne-Mistrih, Mathilde Melin, Marie-France Mirane, Francelise Nadessein, Marie-Josée Pelczar, Véronique Plantier, Céline Rocquet, Magdalena Saint-Marc, Patrick Saint-Martin, Valérie Soter, Ingrid Soubdhan, Jennifer Taillefond, Lambo Velonasy, and Véronique Walter (Guadeloupe), Nicole Ambroisine, Gilda Belrose, Alain Blateau, Patricia Blondel, Isabelle Calmont, Valérie Decatrelle, Michèle Gueneret, Eugénie Jolivet, Isabelle Komla-Soukha, Corinne Plavonil, Marie-andrée Pyram, and Jean-Luc Voluménie (Martinique), Antoine Adenis, Audrey Andrieu, Luisiane Carvalho, Sandy Dabrowski, Céline Delorme, Maryvonne Dueymes, Aniza Fahrasmane, Arthur Felix, Hélène Hilderal, Anne Jolivet, Justine Krajewsky, Véronique Lambert, Thomas Lemaitre, Myriam Livain, Jérémie Pasquier, Marion Petit-Sinturel, Céline Pomar, Léo Pomar, and Stéphanie Rogier (French Guiana), and David Baud (University of Lausanne, Switzerland) and Rebecca Grant (Institut Pasteur de Paris, France) for their critical review of an earlier version of the manuscript., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Jonchère, Laurent, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU de la Martinique [Fort de France]-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université des Antilles (UA)-CHU de la Martinique [Fort de France], and Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur [Paris]

Subjects
0301 basic medicine, Microcephaly, [SDV]Life Sciences [q-bio], Chromosome Disorders, MESH: Amniotic Fluid, Zika virus, Cohort Studies, 0302 clinical medicine, MESH: Pregnancy, Pregnancy, MESH: Martinique, ZikV Infection, Medicine, 030212 general & internal medicine, Pregnancy Complications, Infectious, Prospective cohort study, Guadeloupe, MESH: Cohort Studies, MESH: Chromosome Disorders, MESH: Middle Aged, biology, Zika Virus Infection, Obstetrics, MESH: Infant, Newborn, Pregnancy Outcome, Gestational age, MESH: Congenital Abnormalities, General Medicine, Middle Aged, French Guiana, 3. Good health, [SDV] Life Sciences [q-bio], Fetal Diseases, MESH: Fetal Diseases, MESH: Young Adult, Cohort, Female, Pregnancy Trimesters, Adult, medicine.medical_specialty, Adolescent, MESH: Zika Virus, Article, MESH: Microcephaly, Congenital Abnormalities, Young Adult, 03 medical and health sciences, MESH: Zika Virus Infection, MESH: French Guiana, Humans, MESH: Guadeloupe, Martinique, MESH: Pregnancy Complications, Infectious, Pregnancy outcomes, MESH: Adolescent, Fetus, MESH: Humans, business.industry, Infant, Newborn, MESH: Adult, Zika Virus, Amniotic Fluid, medicine.disease, biology.organism_classification, MESH: Pregnancy Outcome, Confidence interval, 030104 developmental biology, business, MESH: Female, MESH: Pregnancy Trimesters
Abstract

International audience; BACKGROUND:The risk of congenital neurologic defects related to Zika virus (ZIKV) infection has ranged from 6 to 42% in various reports. The aim of this study was to estimate this risk among pregnant women with symptomatic ZIKV infection in French territories in the Americas.METHODS:From March 2016 through November 2016, we enrolled in this prospective cohort study pregnant women with symptomatic ZIKV infection that was confirmed by polymerase-chain-reaction (PCR) assay. The analysis included all data collected up to April 27, 2017, the date of the last delivery in the cohort.RESULTS:Among the 555 fetuses and infants in the 546 pregnancies included in the analysis, 28 (5.0%) were not carried to term or were stillborn, and 527 were born alive. Neurologic and ocular defects possibly associated with ZIKV infection were seen in 39 fetuses and infants (7.0%; 95% confidence interval, 5.0 to 9.5); of these, 10 were not carried to term because of termination of pregnancy for medical reasons, 1 was stillborn, and 28 were live-born. Microcephaly (defined as head circumference more than 2 SD below the mean for sex and gestational age) was detected in 32 fetuses and infants (5.8%), of whom 9 (1.6%) had severe microcephaly (more than 3 SD below the mean). Neurologic and ocular defects were more common when ZIKV infection occurred during the first trimester (24 of 189 fetuses and infants [12.7%]) than when it occurred during the second trimester (9 of 252 [3.6%]) or third trimester (6 of 114 [5.3%]) (P=0.001).CONCLUSIONS:Among pregnant women with symptomatic, PCR-confirmed ZIKV infection, birth defects possibly associated with ZIKV infection were present in 7% of fetuses and infants. Defects occurred more frequently in fetuses and infants whose mothers had been infected early in pregnancy. Longer-term follow-up of infants is required to assess any manifestations not detected at birth. (Funded by the French Ministry of Health and others; ClinicalTrials.gov number, NCT02916732 .).

Published
2018

10. Comparison of 2 real-time PCR assays for diagnosis of Pneumocystis jirovecii pneumonia in human immunodeficiency virus (HIV) and non-HIV immunocompromised patients

Author

Françoise Brancart, Olivier Denis, Kinda Schepers, Marie-Luce Delforge, Isabel Montesinos, and Frédérique Jacobs

Subjects
Male, Microbiology (medical), Human immunodeficiency virus (HIV), HIV Infections, Biology, Pneumocystis carinii, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Pneumocystis pneumonia, Sensitivity and Specificity, law.invention, Immunocompromised Host, law, medicine, Humans, Pneumocystis jirovecii, Polymerase chain reaction, medicine.diagnostic_test, Pneumonia, Pneumocystis, Pneumocystis jirovecii Pneumonia, General Medicine, Middle Aged, medicine.disease, biology.organism_classification, Virology, respiratory tract diseases, Infectious Diseases, Real-time polymerase chain reaction, Bronchoalveolar lavage, Molecular Diagnostic Techniques, Immunology, Female, Bronchoalveolar Lavage Fluid
Abstract

A total of 120 bronchoalveolar lavage specimens from HIV and non-HIV immunocompromised patients, positive for Pneumocystis jirovecii by an "in house" real-time polymerase chain reaction (PCR), were evaluated by the Bio-Evolution Pneumocystis real-time PCR, a commercial quantitative assay. Patients were classified in 2 categories based on clinical and radiological findings: definite and unlikely Pneumocystis pneumonia (PCP). For the "in house" PCR, cycle threshold 34 was established as cut-off value to discriminate definite PCP from unlikely PCP with 65% and 85% of sensitivity and specificity, respectively. For the Bio-Evolution quantitative PCR, a cut-off value of 2.8×10(5)copies/mL was defined with 72% and 82% of sensitivity and specificity, respectively. Overlapped zones of results for definite and unlikely PCP were observed. Quantitative PCR is probably a useful tool for PCP diagnosis. However, for optimal management of PCP in non-HIV immunocompromised patients, operational thresholds should be assessed according to underlying diseases and other clinical and radiological parameters.

Published
2015

11. Early cellular immune response to a new candidate mycobacterial vaccine antigen in childhood tuberculosis

Author

Françoise Mouchet, Virginie Verscheure, Kinda Schepers, Violette Dirix, Camille Locht, Françoise Mascart, and Sophie Lecher

Subjects
Male, Tuberculosis, Adolescent, Mycobacterium tuberculosis, Interferon-gamma, Young Adult, Immune system, T-Lymphocyte Subsets, Humans, Medicine, Interferon gamma, Young adult, Child, Tuberculosis Vaccines, Antigens, Bacterial, Immunity, Cellular, General Veterinary, General Immunology and Microbiology, biology, business.industry, Vaccination, Infant, Newborn, Public Health, Environmental and Occupational Health, Infant, medicine.disease, biology.organism_classification, Virology, Infectious Diseases, Child, Preschool, Immunology, BCG Vaccine, Cytokines, Molecular Medicine, Female, business, Tuberculosis vaccines, BCG vaccine, medicine.drug
Abstract

The search for novel vaccines against tuberculosis (TB) would benefit from in-depths knowledge of the human immune responses to Mycobacterium tuberculosis (Mtb) infection. Here, we characterised in a low TB incidence country, the immune responses to a new candidate vaccine antigen against TB, the heparin-binding haemagglutinin (HBHA), in young children in contact with an active TB case (aTB). Children with no history of BCG vaccination were compared to those vaccinated at birth to compare the initial immune responses to HBHA with secondary immune responses. Fifty-eight children with aTB and 76 with latent TB infection (LTBI) were included and they were compared to 90 non-infected children. Whereas Mtb-infected children globally secreted more interferon-gamma (IFN-γ) in response to HBHA compared to the non-infected children, these IFN-γ concentrations were higher in previously BCG-vaccinated compared to non-vaccinated children. The IFN-γ concentrations were similar in LTBI and aTB children, but appeared to differ qualitatively. Whereas the IFN-γ secretion induced by native methylated and recombinant non-methylated HBHA were well correlated for aTB, this was not the case for LTBI children. Thus, Mtb-infected young children develop IFN-γ responses to HBHA that are enhanced by prior BCG vaccination, indicating BCG-induced priming, thereby supporting a prime-boost strategy for HBHA-based vaccines. The qualitative differences between aTB and LTBI in their HBHA-induced IFN-γ responses may perhaps be exploited for diagnostic purposes.

Published
2015

12. Guillain-Barré Syndrome and Chikungunya: Description of All Cases Diagnosed during the 2014 Outbreak in the French West Indies

Author

Kinda Schepers, Sylvie Cassadou, Ruddy Valentino, André Cabié, Benjamin Madeux, Magali Saint Louis, Mathilde Pircher, Bruno Hoen, Cécile Herrmann-Storck, Fatiha Najioullah, Stephanie Balavoine, Annie Lannuzel, Aissatou Signate, Service des Maladies Infectieuses et Tropicales [Point-à-Pitre, Guadeloupe], CHU Pointe-à-Pitre/Abymes [Guadeloupe], Service de Maladies Infectieuses et Tropicales [Fort-de-France, Martinique], CHU Fort de France-CHU de la Martinique [Fort de France]-Hôpital Pierre Zobda-Quitman [CHU de la Martinique], CHU de la Martinique [Fort de France], Centre d'investigation clinique Antilles-Guyane (CIC - Antilles Guyane), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de la Martinique [Fort de France]-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Université des Antilles - UFR des sciences médicales Hyacinthe Bastaraud (UA UFR SM), Université des Antilles (UA), Laboratoire de virologie [Point-à-Pitre, Guadeloupe], Laboratoire de Virologie [Fort-de-France, Martinique], Service de réanimation [Point-à-Pitre, Guadeloupe], Service de neurologie [Fort-de-France, Martinique], Service de réanimation [Fort-de-France, Martinique], Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS), Service de Neurologie [Pointe-à-Pitre, Guadeloupe], Service de Soins de Suite et Réadaptation [Pointe-à-Pitre, Guadeloupe], Santé publique France - French National Public Health Agency [Saint-Maurice, France], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Centre d'Investigation Clinique Antilles Guyane, Inserm CIC1424

Subjects
Adult, Male, Pediatrics, medicine.medical_specialty, medicine.medical_treatment, viruses, medicine.disease_cause, Guillain-Barre Syndrome, Disease Outbreaks, 03 medical and health sciences, 0302 clinical medicine, Virology, parasitic diseases, medicine, Prevalence, Humans, Martinique, 030212 general & internal medicine, Chikungunya, Guadeloupe, West indies, Mechanical ventilation, Guillain-Barre syndrome, business.industry, Incidence (epidemiology), Incidence, Outbreak, virus diseases, Articles, Middle Aged, medicine.disease, 3. Good health, Infectious Diseases, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Chikungunya Fever, Parasitology, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Female, business, Complication, 030217 neurology & neurosurgery
Abstract

International audience; The Guillain–Barré syndrome (GBS) has been reported as a possible complication of acute chikungunya infection. The chikungunya epidemics, which occurred in Martinique and Guadeloupe in 2014, affected 308,000 people in these two islands. GBS occurred during or immediately after acute chikungunya infection in 13 patients (10 men, three women; mean age: 61 years). Median time from acute chikungunya to GBS onset was 9 days. Twelve patients were treated with intravenous polyvalent immunoglobulins, nine of whom improved within 7 days. Five of 13 patients required mechanical ventilation. Two patients with severe GBS died. At 6 months of follow-up, 7/13 achieved a good functional recovery with no or minor residual symptoms. A 2-fold increase in incidence was observed during the year of chikungunya outbreak. This study supports prior reports suggesting that GBS may be a complication of chikungunya.

Published
2017

13. Deep Dermatophytosis and Inherited CARD9 Deficiency

Author

Lobna Boussofara, Grégory Jouvion, Aomar Ammar-Khodja, Marie-Elisabeth Bougnoux, Merad Boudia, Mohamed Denguezli, Olivier Lortholary, Jean-Laurent Casanova, Frédérique Jacobs, Sophie Cypowyj, Kinda Schepers, Molka Larif, Saad Pathan, Capucine Picard, Bodo Grimbacher, Luyan Liu, Lynda Taibi, Fabrice Chrétien, Gérard Lefranc, Laurent Abel, Rod Hay, Boumediene Guellil, Jean-Christophe Goffard, Carolina Prando, Laurence Michel, Fanny Lanternier, Sylvie Fraitag, Mélanie Migaud, Anne Puel, Quentin B. Vincent, Hervé Bachelez, Omar Boudghene Stambouli, and Véronique Del Marmol

Subjects
Adult, Male, Genes, Recessive, medicine.disease_cause, Article, 03 medical and health sciences, symbols.namesake, Africa, Northern, Tinea, Humans, Medicine, In patient, Immunodeficiency, Aged, 030304 developmental biology, Aged, 80 and over, 0303 health sciences, Interleukin-6, 030306 microbiology, business.industry, Homozygote, Deep dermatophytosis, General Medicine, Middle Aged, medicine.disease, Founder Effect, Pedigree, 3. Good health, CARD Signaling Adaptor Proteins, Mutation, Immunology, Mendelian inheritance, symbols, Dermatophyte, Female, North african, business, Founder effect
Abstract

Deep dermatophytosis is a severe and sometimes life-threatening fungal infection caused by dermatophytes. It is characterized by extensive dermal and subcutaneous tissue invasion and by frequent dissemination to the lymph nodes and, occasionally, the central nervous system. The condition is different from common superficial dermatophyte infection and has been reported in patients with no known immunodeficiency. Patients are mostly from North African, consanguineous, multiplex families, which strongly suggests a mendelian genetic cause.We studied the clinical features of deep dermatophytosis in 17 patients with no known immunodeficiency from eight unrelated Tunisian, Algerian, and Moroccan families. Because CARD9 (caspase recruitment domain-containing protein 9) deficiency has been reported in an Iranian family with invasive fungal infections, we also sequenced CARD9 in the patients.Four patients died, at 28, 29, 37, and 39 years of age, with clinically active deep dermatophytosis. No other severe infections, fungal or otherwise, were reported in the surviving patients, who ranged in age from 37 to 75 years. The 15 Algerian and Tunisian patients, from seven unrelated families, had a homozygous Q289X CARD9 allele, due to a founder effect. The 2 Moroccan siblings were homozygous for the R101C CARD9 allele. Both alleles are rare deleterious variants. The familial segregation of these alleles was consistent with autosomal recessive inheritance and complete clinical penetrance.All the patients with deep dermatophytosis had autosomal recessive CARD9 deficiency. Deep dermatophytosis appears to be an important clinical manifestation of CARD9 deficiency. (Funded by Agence Nationale pour la Recherche and others.).

Published
2013

14. Heparin-binding, Hemagglutinin-specific IFN-γ Synthesis at the Site of Infection during Active Tuberculosis in Humans

Author

Sabine D Allard, T. Mark Doherty, Sammy Place, Nour de San, Camille Locht, Anne Dediste, Annie Drowart, Jean-Michel Hougardy, Sophie Lecher, Virginie Verscheure, Violette Dirix, Olivier Michel, Kinda Schepers, Françoise Mascart, and Clinical sciences

Subjects
Adult, Male, Pulmonary and Respiratory Medicine, Tuberculosis, Adolescent, medicine.medical_treatment, Enzyme-Linked Immunosorbent Assay, heparin-binding hemagglutinin, CD8-Positive T-Lymphocytes, Critical Care and Intensive Care Medicine, T-Lymphocytes, Regulatory, Mycobacterium tuberculosis, Interferon-gamma, Young Adult, Immune system, Antigen, Lectins, Intensive care, Humans, Medicine, Interferon gamma, Prospective Studies, Tuberculosis, Pulmonary, Aged, Aged, 80 and over, Antigens, Bacterial, local interferon-gamma, biology, business.industry, Regulatory T cells, Tuberculosis, Pleural, Middle Aged, Hemagglutinin, Flow Cytometry, biology.organism_classification, medicine.disease, Cytokine, Immunology, Female, business, Bronchoalveolar Lavage Fluid, medicine.drug
Abstract

Tuberculosis (TB) remains a major cause of mortality. A better understanding of the immune responses to mycobacterial antigens may be helpful to develop improved vaccines and diagnostics.The mycobacterial antigen heparin-binding hemagglutinin (HBHA) induces strong IFN-γ responses by circulating lymphocytes from subjects latently infected with Mycobacterium tuberculosis, and low responses associated with CD4(+) regulatory T (Treg) cells in patients with TB. Here, we investigated HBHA-specific IFN-γ responses at the site of the TB disease.Bronchoalveolar lavages, pleural fluids, and blood were prospectively collected from 61 patients with a possible diagnosis of pulmonary or pleural TB. HBHA-specific IFN-γ production was analyzed by flow cytometry and ELISA. The suppressive effect of pleural Treg cells was investigated by depletion experiments.The percentages of HBHA-induced IFN-γ(+) alveolar and pleural lymphocytes were higher for pulmonary (P0.0001) and for pleural (P0.01) TB than for non-TB controls. Local CD4(+) and CD8(+) T cells produced the HBHA-specific IFN-γ. This local secretion was not suppressed by Treg lymphocytes, contrasting with previously reported data on circulating lymphocytes.Patients with TB display differential effector and regulatory T-cell responses to HBHA in local and circulating lymphocytes with a predominant effector CD4(+) and CD8(+) response locally, compared with a predominant Treg response among circulating lymphocytes. These findings may be helpful for the design of new vaccines against TB, and the detection of HBHA-specific T cells at the site of the infection may be a promising tool for the rapid diagnosis of active TB.

Published
2010

15. Latent tuberculosis in a newborn: diagnostic challenges

Author

Mahavir Singh, Iris De Schutter, Kinda Schepers, Anne Malfroot, Françoise Mascart, and Pediatrics

Subjects
Adult, Pediatrics, medicine.medical_specialty, Tuberculosis, Antitubercular Agents, Tuberculin, Disease, Immunologic Tests, Latent Tuberculosis, Pregnancy, Humans, Medicine, Latent tuberculosis, Tuberculin Test, business.industry, Transmission (medicine), Incidence (epidemiology), Infant, Newborn, Tuberculosis, Pleural, medicine.disease, Infectious Disease Transmission, Vertical, Neonatal infection, Early Diagnosis, Pediatrics, Perinatology and Child Health, Immunology, Chemoprophylaxis, Female, business
Abstract

In countries where the incidence of tuberculosis is low, perinatal tuberculosis is seldom diagnosed. With increasing numbers of human immunodeficiency virus-infected people and increasing immigrant population from high tuberculosis incidence countries, one might expect perinatal tuberculosis to become more frequent. Early recognition of newborns at risk for perinatal tuberculosis infection is of utmost importance to prevent disease by chemoprophylaxis. We describe a case of latent perinatal tuberculosis infection in a newborn infected from a mother with extrapulmonary primary tuberculosis. Tuberculin skin test was negative, and latent tuberculosis infection was eventually diagnosed by specific immunological tests. We discuss the difficulties in diagnosis of recent tuberculosis infection in neonates and infants, and the risk factors for vertical transmission of tuberculosis, which need to be taken into account in considering the need for chemoprophylaxis in the newborn. Although perinatal TB infection is a rare condition and diagnosis is difficult due to poor diagnostic testing in pregnancy and newborns, a high index of suspicion is needed to limit the diagnostic delay and to avoid progression to perinatal TB disease.

Published
2010

16. Contribution of a heparin-binding haemagglutinin interferon-gamma release assay to the detection of Mycobacterium tuberculosis infection in HIV-infected patients: comparison with the tuberculin skin test and the QuantiFERON®-TB Gold In-tube

Author

Kinda Schepers, Fanny Domont, Myriam Libin, Chloe Wyndham-Thomas, Violette Dirix, Marc Loyens, Camille Locht, Jean-Christophe Goffard, Marc Hildebrand, Charlotte Martin, Jean-Paul Van Vooren, and Françoise Mascart

Subjects
Adult, Male, medicine.medical_specialty, Heparin-binding haemagglutinin, Interferon gamma release assay, Interferon-gamma release assay, Tuberculin, HIV Infections, Mycobacterium tuberculosis, Interferon-gamma, Young Adult, Medical microbiology, Latent Tuberculosis, Internal medicine, Lectins, medicine, Humans, Multiplex, Aged, QuantiFERON®-TB Gold In-Tube, biology, Latent tuberculosis, Tuberculin skin test, business.industry, Human immunodeficiency virus, Tuberculin Test, Incidence (epidemiology), Incidence, virus diseases, Sciences bio-médicales et agricoles, Middle Aged, biology.organism_classification, medicine.disease, Active tuberculosis, Infectious Diseases, Immunology, HIV-1, Female, Interferon-gamma Release Tests, business, Multiplex Polymerase Chain Reaction, Research Article, Human
Abstract

The screening and treatment of latent tuberculosis (TB) infection reduces the risk of progression to active disease and is currently recommended for HIV-infected patients. The aim of this study is to evaluate, in a low TB incidence setting, the potential contribution of an interferon-gamma release assay in response to the mycobacterial latency antigen Heparin-Binding Haemagglutinin (HBHA-IGRA), to the detection of Mycobacterium tuberculosis infection in HIV-infected patients., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2015

17. Long-Incubation-Time Gamma Interferon Release Assays in Response to Purified Protein Derivative, ESAT-6, and/or CFP-10 for the Diagnosis of Mycobacterium tuberculosis Infection in Children

Author

Kinda Schepers, P. Geurts, Virginie Verscheure, Violette Dirix, Françoise Mascart, J. P. Van Vooren, K. Jotzo, Mahavir Singh, I. De Schutter, Françoise Mouchet, Pediatrics, and Growth and Development

Subjects
Microbiology (medical), childhood active tuberculosis, Male, Tuberculosis, Time Factors, Adolescent, Clinical Biochemistry, Immunology, Tuberculin, complex mixtures, Sensitivity and Specificity, Mycobacterium tuberculosis, Antigen, Tuberculosis diagnosis, Bacterial Proteins, Diagnostic Laboratory Immunology, Immunology and Allergy, Medicine, Humans, Prospective Studies, Prospective cohort study, Child, Children, Antigens, Bacterial, CFP-10, biology, business.industry, Mycobacterium tuberculosis infection, Infant, medicine.disease, biology.organism_classification, bacterial infections and mycoses, LTBI, Child, Preschool, ESAT-6, Female, business, Interferon-gamma Release Tests
Abstract

The diagnosis of childhood active tuberculosis (aTB) and latentMycobacterium tuberculosis(M. tuberculosis) infection (LTBI) remains a challenge, and the replacement of tuberculin skin tests (TST) with commercialized gamma interferon (IFN-γ) release assays (IGRA) is not currently recommended. Two hundred sixty-six children between 1 month and 15 years of age, 214 of whom were at risk of recentM. tuberculosisinfection and 51 who were included as controls, were prospectively enrolled in our study. According to the results of a clinical evaluation, TST, chest X ray, and microbiological assessment, each children was classified as noninfected, having LTBI, or having aTB. Long-incubation-time purified protein derivative (PPD), ESAT-6, and CFP-10 IGRA were performed and evaluated for their accuracy in correctly classifying the children. Whereas both TST and PPD IGRA were suboptimal for detecting aTB, combining the CFP-10 IGRA with a TST or with a PPD IGRA allowed us to detect all the children with aTB with a specificity of 96% for children who were positive for the CFP-10 IGRA. Moreover, the combination of the CFP-10 IGRA and PPD IGRA detected 96% of children who were eventually classified as having LTBI, but a strong IFN-γ response to CFP-10 (defined as >500 pg/ml) was highly suggestive of aTB, at least among the children who were

Published
2014

18. Acyclovir-resistant herpes simplex encephalitis in a patient treated with anti-tumor necrosis factor-α monoclonal antibodies

Author

Marie-Luce Delforge, Antonio Hernandez, Kinda Schepers, Robert Snoeck, Frédérique Jacobs, Pierre Fiten, Philippe David, Graciela Andrei, Ghislain Opdenakker, Jean Christophe Bier, and Sarah Gillemot

Subjects
Necrosis, medicine.drug_class, viruses, Population, Acyclovir, Monoclonal antibody, medicine.disease_cause, Antibodies, Monoclonal, Humanized, Virus, Cerebrospinal fluid, Virology, Drug Resistance, Viral, medicine, Adalimumab, Humans, Simplexvirus, education, Cerebrospinal Fluid, education.field_of_study, business.industry, Middle Aged, medicine.disease, Infectious Diseases, Herpes simplex virus, Treatment Outcome, Immunology, Female, Encephalitis, Herpes Simplex, medicine.symptom, business, Encephalitis, Immunosuppressive Agents, medicine.drug, Foscarnet
Abstract

Herpes simplex virus is the most common cause of severe sporadic encephalitis. We report a case of herpes simplex type 1-encephalitis in a 50-year-old woman receiving anti-tumor necrosis factor-α monoclonal antibodies adalimumab. Although she was an acyclovir naive patient, a mixed viral population (wild-type and acyclovir-resistant bearing a thymidine-kinase mutation) was identified in the cerebrospinal fluid. The virus in cerebrospinal fluid evolved and a second thymidine-kinase mutant virus emerged. Combined foscavir and acyclovir treatment resolved the herpes simplex encephalitis. To our knowledge, this is the first report of acyclovir-resistant herpes simplex encephalitis in a patient treated with adalimumab.

Published
2013

19. IL-12Rβ1 deficiency and disseminated Mycobacterium tilburgii disease

Author

Jean-Paul Van Vooren, Liliane Schandené, Jacinta Bustamante, Françoise Mascart, Jean Cyr Yombi, Jean-Laurent Casanova, Frédérique Jacobs, Kinda Schepers, Maylis de Suremain, and Jean-Christophe Goffard

Subjects
Adult, medicine.medical_specialty, Immunology, Mycobacterium Infections, Nontuberculous, Disease, Biology, CD8-Positive T-Lymphocytes, Mycobacterium, Medical microbiology, Recurrence, medicine, Immunology and Allergy, Humans, Disseminated disease, Receptor, Gene, Alleles, Granuloma, Mycobacterium tilburgii, Receptors, Interleukin-12, medicine.disease, Killer Cells, Natural, Mutation, Female, Lymph Nodes
Abstract

Mycobacterium tilburgii rarely causes disseminated disease. We describe a case of M. tilburgii infection in an otherwise healthy 33-year-old woman, who was found to carry bi-allelic mutations of the gene encoding the β1 chain of the IL-12 receptor.

Published
2013

20. Diffuse pancreatic lesion mimicking autoimmune pancreatitis in an HIV-infected patient: successful treatment by antiretroviral therapy

Author

Gil, Leurquin-Sterk, Kinda, Schepers, Myriam, Delhaye, Serge, Goldman, Laurine, Verset, and Celso, Matos

Subjects
Adult, Diagnosis, Differential, Radiography, Diffusion Magnetic Resonance Imaging, Treatment Outcome, Anti-Retroviral Agents, Pancreatitis, Humans, Pancreatic Diseases, Female, HIV Infections, Autoimmune Diseases
Abstract

Pancreatitis is a common complication of acquired immunodeficiency syndrome. The most common causes of acute pancreatitis in an HIV population are medication and opportunistic infections.We report the case of a young, untreated, HIV-infected female who presented with acute pancreatitis of unknown origin. Unique to this case are the autoimmune pancreatitis-like features on imaging studies associated with renal mass-like lesions and lymph node involvement as well as the favorable outcome using highly active antiretroviral therapy alone.In HIV-infected patients, acute pancreatitis may present on imaging studies as autoimmune pancreatitis. In patients with uncontrolled HIV infection and imaging studies suggestive of autoimmune pancreatitis, direct HIV-related inflammation should be considered after exclusion of all other causes of pancreatitis.

Published
2011

21. Revisiting Human IL-12R beta 1 Deficiency A Survey of 141 Patients From 30 Countries

Author

Chris Nieuwhof, Yu-Lung Lau, Klara Frecerova, Suzanne T. Anderson, Vas Novelli, Dinakantha S. Kumararatne, Figen Dogu, Matías Oleastro, Barbara Pietrucha, Melike Keser, Xi-qiang Yang, Anete Sevciovic Grumach, Renate Blüetters-Sawatzki, Necil Kutukculer, Lucile Janniere, Sigifredo Pedraza, María Isabel Gaillard, Isabel Caragol, Ruth Aldana, Parisa Adimi, Orchidée Filipe-Santos, Liliane Schandené, Ludovic de Beaucoudrey, Alejandra King, Rainer Doffinger, Sergio D. Rosenzweig, Dewton Moraes-Vasconcelos, Guzide Aksu, Pamela Pui Wah Lee, Abdulaziz Al-Ghonaium, Aurélie Cobat, David Tuerlinckx, Michael Levin, Mohammed Bejaoui, Nevin Hatipoğlu, Kinda Schepers, Francisco J. Espinosa-Rosales, Andrea Bernasconi, Aydan Ikinciogullari, Jacob Levy, Mohammad S. Ehlayel, Małgorzata Pac, Darko Richter, David A. Lammas, David B. Lewis, Janine Reichenbach, Gonul Tanir, Joachim Freihorst, Tuba Turul Ozgur, Sulaiman Al-Ajaji, Masao Matsuoka, Jacqueline Feinberg, Smita Y. Patel, Abdullah A. Alangari, Judith Yancoski, Andrew Exley, Saleh Al-Muhsen, Ridha Barbouche, Tatsunori Sakai, Ana Codoceo, Xiao-Fei Kong, Claire-Anne Siegrist, Jamila El Baghdadi, Bernhard Fleckenstein, Xiaochuan Wang, Sami Al-Hajjar, Ingrid Müller-Fleckenstein, Capucine Picard, Gabriela Castro, Jean-Laurent Casanova, Tong-Xin Chen, Frans De Baets, Namik Ozbek, Alain Fischer, Liliana Bezrodnik, Aileen C. Cohen, Ariane Chapgier, Olle Jeppsson, Imen Ben-Mustapha, Saniye Gülle, Revathi Raj, Françoise Mascart, Arina Samarina, Davood Mansouri, Nima Parvaneh, Yoann Rose, Koon Wing Chan, Yuanyuan Xie, Alberto José da Silva Duarte, Ahmed Aziz Bousfiha, Claire Fieschi, Steven M. Holland, Meteran Ozen, Walther H. Haas, Stéphanie Boisson-Dupuis, Carlos Rodríguez-Gallego, Li-Ping Jiang, Ayper Somer, Suna Asilsoy, André Efira, Yaryna Boyko, Laurent Abel, Setareh Mamishi, Xiaohong Wang, Ulrich Baumann, Filomeen Haerynck, Zobaida Alsum, Kuender D Yang, Jacinta Bustamante, Daniela Di Giovani, Ozden Sanal, Maylis de Suremain, Yildiz Camcioglu, Christiane Vermylen, Ben-Zion Garty, Jutta Bernhöft, Ivelisse Natera, Suliman Al-Jumaah, Chaomin Zhu, David Nadal, Husn H. Frayha, Cigdem Aydogmus, Génétique Humaine des Maladies Infectieuses (Inserm U980), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5), Université Paris Descartes - Faculté de Médecine (UPD5 Médecine), Université Paris Descartes - Paris 5 (UPD5), St. Giles Laboratory of Human Genetics of Infectious Diseases, The Rockefeller University, Department of Pediatrics, King Faysal Hospital and Research center, Coll Med, Prince Naif Ctr Immunol Res, King Saud University [Riyadh] (KSU), Coll Med, Dept Pediat, IFR Necker-Enfants Malades (IRNEM), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Developpement Normal et Pathologique du Système Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance Publique-Hôpitaux de Paris, Service d'Immunologie et d'Hématologie Pédiatrique, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Sami Ulus Children Hlth & Dis Training & Res Ctr, Ankara, Dept Pediat, King Fahad Natl Guard Hosp, Shahid Beheshti University of Medical Sciences, Laboratoire d'immunologie clinique [Institut Pasteur de Tunis], Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Dept Immunol, Juan Pedro Garrahan Natl Hosp Pediat, Schneider Childrens Med Ctr Israel, Hospital Universitario de Gran Canaria Dr Negrin, Immunol Lab, Vall d'Hebron University Hospital [Barcelona], Ege University, Dept Clin Biochem & Immunol, Cambridge University Hospitals NHS Foundation Trust, Addenbrookes Hospital, Inflammat & Immunol Unit, Dept Pathol, Papworth Hosp NHS Fdn Trust, Inflammat & Immunol Unit, Dept Thorac Med, Karolinska University Hospital [Stockholm], Univ Childrens Hosp Zurich, Dept Infect Dis, Lviv Reg Specialized Childrens Hosp, St Marys Hosp, Imperial Coll Sch Med, Childrens Mem Hlth Inst, Immunobiol Clin, Hop Erasme, Université Libre de Bruxelles [Bruxelles] (ULB), Immunodeficiency Dept, Hop Erasme, Lab Vaccinol & Mucosal Immun, Dept Internal Med, Natl Hosp Org Kumamoto Med Ctr, Laboratory of Virus Control, Kyoto University [Kyoto], University of Geneva [Switzerland], Dept Int Relat, Minist Hlth Slovaquie, Dept Pediat Hematol & Oncol, Dept Pediat Pulmonol & Neonatol, Hannover Medical School [Hannover] (MHH), Univ Hosp Ctr Rebro, Dept Pediat Pulmonol & Immunol, Ghent University Hospital, Infect Dis Unit, Great Ormond St Hosp Sick Children, Ctr Immune Regulat, Univ Birmingham, Sch Med, MRC, Cliniques Universitaires Saint-Luc [Bruxelles], Dept Pediat, Mt Godinne Clin, Catholic University Louvain, Dept Internal Med, Div Clin & Expt Immunol, Maastricht University [Maastricht], Dept Infect Dis Epidemiol, Unit Resp Infect, Robert Koch Institute [Berlin] (RKI), Univ Erlangen Nurnberg, Pediatric Department, Ben-Gurion University of the Negev (BGU), Stanford University [Stanford], Lab Clin Infect Dis, NIAID, TMRC, NIH, Bethesda, Dept Pediat Allergy, Chang Gung Childrens Hosp, Dept Clin Immunol, Fudan University [Shanghai], Clin Immunol Lab, Chongqing Medical University, Dept Paediat & Adolescent Med, The University of Hong Kong (HKU), Universidade Federal da Bahia (UFBA), Dept Pediat,Sch Med, Shanghai Jiao Tong University [Shanghai], Univ Hosp Caracas, Hosp Ninos Luis Calvo Mackenna, Childrens Hosp Ricardo Gutierrez Buenos Aires, Dept Dermatol, Lab Invest Dermatol & Immunodeficiencies, Universidade de São Paulo (USP), Dept Pulmonol, Childrens Hosp Federico Gomez, Natl Inst Pediat, Immunodeficiencies Res Unit, Mexico City, DF, Mexico, Natl Ctr Bone Marrow Graft, Tunis, Clinical Immunology Unit, Ibn Rochd Hospital King Hassan II University-Aïn Chok, Genetics Unit, Military Hospital Mohamed V, Baskent universitesi, Dept Pediat Infect Dis & Clin Immunol, Istanbul Univ, Dept Pediat Infect Dis & Immunol, Bakirkoy Matern & Childrens State Hosp, Dr Behcet Uz Children Res & Training Hosp, Department of Pediatrics, Infectious Diseases, Cerrahpacsa Medical School, Hacettepe University = Hacettepe Üniversitesi, Fac Med Malatya, Tehran University of Medical Sciences (TUMS), Dept Biochem, Minist Hlth Mexico, Department of Pediatric and Adolescent Medicine, Hamad medical corporation, Laboratoire d'immunologie, Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Service d'immuno-hématologie pédiatrique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Çocuk Sağlığı ve Hastalıkları, Ege Üniversitesi, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Rockefeller University [New York], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Shahid Beheshti University of Medical Sciences [Tehran] (SBUMS), Shahid Beheshti University, Université libre de Bruxelles (ULB), Université Catholique de Louvain = Catholic University of Louvain (UCL), Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Stanford University, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Siegrist, Claire-Anne, Génétique Humaine des Maladies Infectieuses ( Inserm U980 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ), Université Paris Descartes - Faculté de Médecine ( UPD5 Médecine ), Université Paris Descartes - Paris 5 ( UPD5 ), King Saud University [Riyadh] ( KSU ), IFR Necker-Enfants Malades ( IRNEM ), Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP), Institut Pasteur de Tunis-Réseau International des Instituts Pasteur ( RIIP ), Université Libre de Bruxelles [Bruxelles] ( ULB ), Hannover Medical School [Hannover] ( MHH ), Robert Koch Institute [Berlin] ( RKI ), Ben-Gurion University of the Negev ( BGU ), The University of Hong Kong ( HKU ), Universidade Federal da Bahia ( UFBA ), Universidade de São Paulo ( USP ), Tehran University of Medical Sciences, Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Interne Geneeskunde, RS: CARIM School for Cardiovascular Diseases, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - (SLuc) Service d'hématologie et d'oncologie pédiatrique, and UCL - (MGD) Service de pédiatrie

Subjects
Male, Proband, [SDV]Life Sciences [q-bio], Mycobacterium tuberculosis/isolation & purification, Mycobacterium bovis/isolation & purification, Disease, Interleukin-12 Receptor beta 1 Subunit - deficiency, genetics, Cytokines - blood, 0302 clinical medicine, Mycobacterium bovis - isolation & purification, Child, 0303 health sciences, ddc:618, Interleukin-12 Receptor beta 1 Subunit/*deficiency/genetics, Nontuberculous Mycobacteria/isolation & purification, biology, Age Factors, Nontuberculous Mycobacteria, Mycobacterium Infections, Nontuberculous/epidemiology/genetics, General Medicine, Middle Aged, Mycobacterium bovis, Penetrance, Mycobacteria, Atypical - isolation & purification, 3. Good health, Child, Preschool, Cytokines, Female, medicine.symptom, Adult, medicine.medical_specialty, Tuberculosis, Adolescent, Genotype, Mycobacterium Infections, Nontuberculous, Mycobacterium tuberculosis - isolation & purification, Cytokines/blood, Asymptomatic, Article, Mycobacterium tuberculosis, 03 medical and health sciences, Internal medicine, General & Internal Medicine, Interleukin-12 Receptor beta 1 Subunit, medicine, Humans, Survival analysis, 030304 developmental biology, Mycobacterium Infections, Atypical - epidemiology, genetics, [ SDV ] Life Sciences [q-bio], business.industry, Infant, Newborn, Infant, biology.organism_classification, medicine.disease, Survival Analysis, Immunology, Nontuberculous mycobacteria, business, 030215 immunology
Abstract

WOS: 000283840300002, PubMed ID: 21057261, Interleukin-12 receptor beta 1 (IL-12R beta 1) deficiency is the most common form of Mendelian susceptibility to mycobacterial disease (MSMD). We undertook an international survey of 141 patients from 102 kindreds in 30 countries. Among 102 probands, the first infection occurred at a mean age of 2.4 years. In 78 patients, this infection was caused by Bacille Calmette-Guerin (BCG; n = 65), environmental mycobacteria (EM; also known as atypical or nontuberculous mycobacteria) (n = 9) or Mycobacterium tuberculosis (n = 4). Twenty-two of the remaining 24 probands initially presented with nontyphoidal, extraintestinal salmonellosis. Twenty of the 29 genetically affected sibs displayed clinical signs (69%); however 8 remained asymptomatic (27%). Nine nongenotyped sibs with symptoms died. Recurrent BCG infection was diagnosed in 15 cases, recurrent EM in 3 cases, recurrent salmonellosis in 22 patients. Ninety of the 132 symptomatic patients had infections with a single microorganism. Multiple infections were diagnosed in 40 cases, with combined mycobacteriosis and salmonellosis in 36 individuals. BCG disease strongly protected against subsequent EM disease (p = 0.00008). Various other infectious diseases occurred, albeit each rarely, yet candidiasis was reported in 33 of the patients (23%). Ninety-nine patients (70%) survived, with a mean age at last follow-up visit of 12.7 years +/- 9.8 years (range, 0.5-46.4 yr). IL-12R beta 1 deficiency is characterized by childhood-onset mycobacteriosis and salmonellosis, rare recurrences of mycobacterial disease, and more frequent recurrence of salmonellosis. The condition has higher clinical penetrance, broader susceptibility to infections, and less favorable outcome than previously thought., Rockefeller University Center for Clinical and Translational Science [5UL1RR024143-03]; Rockefeller University; ANRFrench National Research Agency (ANR); PHRC; EUEuropean Union (EU) [LHSP-CT-2005-018736]; BNP Paribas Foundation; Dana Foundation; March of DimesMarch of Dimes; Fondation pour la Recherche MedicaleFondation pour la Recherche Medicale; Howard Hughes Medical InstituteHoward Hughes Medical Institute, The St. Giles Laboratory of Human Genetics of Infectious Diseases is supported by grants from The Rockefeller University Center for Clinical and Translational Science grant number 5UL1RR024143-03, and The Rockefeller University, ANR, PHRC, EU (LHSP-CT-2005-018736), BNP Paribas Foundation, the Dana Foundation, and the March of Dimes. LDB is supported by the Fondation pour la Recherche Medicale as part of the PhD program of Pierre et Marie Curie University (Paris, France). JLC was an International Scholar of the Howard Hughes Medical Institute.

Published
2010

22. Heparin-binding-hemagglutinin-induced IFN-gamma release as a diagnostic tool for latent tuberculosis

Author

Annie Drowart, Véronique Lechevin, Françoise Mascart, T. Mark Doherty, Sammy Place, Jean-Paul Van Vooren, Jean-Michel Hougardy, Virginie Verscheure, Anne-Sophie Debrie, Kinda Schepers, and Camille Locht

Subjects
Male, lcsh:Medicine, Respiratory Medicine/Respiratory Infections, Infectious Diseases/Bacterial Infections, Antigens, Bacterial -- chemistry, Lectins, Interferon gamma, lcsh:Science, Evidence-Based Healthcare/Methods for Diagnostic and Therapeutic Studies, Aged, 80 and over, Multidisciplinary, Latent tuberculosis, biology, Sciences bio-médicales et agricoles, Middle Aged, Heparin -- chemistry, Vaccination, Interferon-gamma -- metabolism, Hemagglutinins, Female, medicine.drug, Tuberculosis -- diagnosis, Research Article, Adult, Tuberculosis, Adolescent, Mycobacterium tuberculosis -- metabolism, Mycobacterium tuberculosis, Interferon-gamma, Tuberculosis diagnosis, Antigen, Immunology/Immunity to Infections, medicine, Humans, Aged, Antigens, Bacterial, business.industry, Heparin, Tuberculin Test, Infectious Diseases/Respiratory Infections, lcsh:R, Lectins -- chemistry, medicine.disease, biology.organism_classification, bacterial infections and mycoses, Virology, Hemagglutinins -- chemistry, Immunology, Immunology/Immune Response, lcsh:Q, Tuberculosis -- metabolism, business, Mycobacterium
Abstract

BACKGROUND: The detection of latent tuberculosis infection (LTBI) is a major component of tuberculosis (TB) control strategies. In addition to the tuberculosis skin test (TST), novel blood tests, based on in vitro release of IFN-gamma in response to Mycobacterium tuberculosis-specific antigens ESAT-6 and CFP-10 (IGRAs), are used for TB diagnosis. However, neither IGRAs nor the TST can separate acute TB from LTBI, and there is concern that responses in IGRAs may decline with time after infection. We have therefore evaluated the potential of the novel antigen heparin-binding hemagglutinin (HBHA) for in vitro detection of LTBI. METHODOLOGY AND PRINCIPAL FINDINGS: HBHA was compared to purified protein derivative (PPD) and ESAT-6 in IGRAs on lymphocytes drawn from 205 individuals living in Belgium, a country with low TB prevalence, where BCG vaccination is not routinely used. Among these subjects, 89 had active TB, 65 had LTBI, based on well-standardized TST reactions and 51 were negative controls. HBHA was significantly more sensitive than ESAT-6 and more specific than PPD for the detection of LTBI. PPD-based tests yielded 90.00% sensitivity and 70.00% specificity for the detection of LTBI, whereas the sensitivity and specificity for the ESAT-6-based tests were 40.74% and 90.91%, and those for the HBHA-based tests were 92.06% and 93.88%, respectively. The QuantiFERON-TB Gold In-Tube (QFT-IT) test applied on 20 LTBI subjects yielded 50% sensitivity. The HBHA IGRA was not influenced by prior BCG vaccination, and, in contrast to the QFT-IT test, remote (>2 years) infections were detected as well as recent (, Journal Article, Research Support, Non-U.S. Gov't, info:eu-repo/semantics/published

Published
2007

24. Risk Stratification of Latent Tuberculosis Defined by Combined Interferon Gamma Release Assays

Author

Gaelle Pottier, Camille Locht, Virginie Verscheure, Sophie Lecher, Françoise Mascart, T. Mark Doherty, Kinda Schepers, Florence Bonkain, Véronique Corbière, and Internal Medicine Specializations

Subjects
Bacterial Diseases, Anatomy and Physiology, lcsh:Medicine, Adaptive Immunity, Bacterial Proteins -- immunology, RISK STRATIFICATION, 0302 clinical medicine, Immune Physiology, Lectins, Immunologie, Interferon gamma, 030212 general & internal medicine, lcsh:Science, Immune Response, Lectins -- immunology, 0303 health sciences, Multidisciplinary, biology, Latent tuberculosis, Risk Assessment -- methods, 3. Good health, Interferon-gamma Release Tests -- methods, Infectious Diseases, Cytokines, Medicine, Pneumologie, Interferon-gamma Release Tests, medicine.drug, Research Article, Tuberculosis, Immunology, Tuberculin, Microbiology, Risk Assessment, Mycobacterium, Mycobacterium tuberculosis, 03 medical and health sciences, Immune system, Antigen, Bacterial Proteins, Latent Tuberculosis, Antigens, Bacterial -- immunology, medicine, Humans, Biology, 030304 developmental biology, Retrospective Studies, Antigens, Bacterial, business.industry, lcsh:R, Immunity, bacterial infections and mycoses, biology.organism_classification, medicine.disease, Virology, Sciences biomédicales, Latent Tuberculosis -- classification -- diagnosis, Immune System, gamma release assays, lcsh:Q, Clinical Immunology, business
Abstract

Most individuals infected with Mycobacterium tuberculosis develop latent tuberculosis infection (LTBI). Some may progress to active disease and would benefit from preventive treatment yet no means currently exists to predict who will reactivate. Here, we provide an approach to stratify LTBI based on IFN-γ responses to two antigens, the recombinant Early-Secreted Antigen Target-6 (rESAT-6) and the latency antigen Heparin-Binding Haemagglutinin (HBHA)., Journal Article, Research Support, Non-U.S. Gov't, SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2012
Full Text
View/download PDF

26. Active Surveillance Program to Increase Awareness on Invasive Fungal Diseases: the French RESSIF Network (2012 to 2018)

Author

Bretagne, Stéphane, Sitbon, Karine, Desnos-Ollivier, Marie, Garcia-Hermoso, Dea, Letscher-Bru, Valérie, Cassaing, Sophie, Millon, Laurence, Morio, Florent, Gangneux, Jean-Pierre, Hasseine, Lilia, Favennec, Loïc, Cateau, Estelle, Bailly, Eric, Moniot, Maxime, Bonhomme, Julie, Desbois-Nogard, Nicole, Chouaki, Taieb, Paugam, André, Bouteille, Bernard, Pihet, Marc, Dalle, Frédéric, Eloy, Odile, Sasso, Milène, Demar, Magalie, Mariani-Kurkdjian, Patricia, Robert, Vincent, Lortholary, Olivier, Dromer, Françoise, French Mycoses Study Group, The, Damiani, Céline, Dupont, Hervé, Maizel, Julien, Totet, Anne, Bouchara, Jean-Philippe, Bellanger, Anne Pauline, Berceanu, Ana, Navellou, Jean Christophe, Scherer, Emeline, Abboud, Philippe, Aznar, Christine, Blanchet, Denis, Djossou, Félix, Cayot, Sophie, Garrouste, Cyril, Lesens, Olivier, Moluçon, Cécile, Nourrisson, Céline, Poirier, Philippe, Bailly, Éloïse, Basmaciyan, Louise, Belaid, Bouhemad, Blot, Mathieu, Caillot, Denis, Charles, Pierre-Emmanuel, Quenot, Jean Pierre, Amazan, Emmanuelle, Baubion, Emilie, Cabie, André, Chabartier, Cyrille, Deligny, Christophe, Emal, Violaine, Flechelle, Olivier, Hatchuel, Yves, Le Govic, Yohann, Merle, Harold, Miossec, Charline, Turmel, Jean-Marie, Valentino, Ruddy, Durieux, Marie-Fleur, Turlure, Pascal, Mercier, Victor, Gari-Toussaint, Martine, Risso, Karine, Simon, Loïc, Bretonnière, Cedric, Boutoille, David, Gastinne, Thomas, Lakhal, Karim, Launay, Elise, Lepoivre, Thierry, Peterlin, Pierre, Rialland, Fanny, Le Pape, Patrice, Canoui, Etienne, Dahane, Naima, Kerneis, Solène, Angebault, Cécile, Bougnoux, Marie-Elisabethh, Guennouni, Nadia, Lanternier, Fanny, Neven, Bennedicte, Oualha, Mehdi, Scemla, Anne, Sitterlé, Emilie, Suarez, Felipe, Toubiana, Julie, Bonacorci, Stéphane, Alanio, Alexandre, Bergeron, Anne, Denis, Blandine, Gits-Muselli, Maud, Hamane, Samia, Touratier, Sophie, Chaumeil, Christine, Merabet, Lilia, Claudéon, Joelle, Curlier, Elodie, Galantine, Valérie, Gallois, Jean Claude, Markowicz, Samuel, Nicolas, Muriel, Musson, Pascal, Schepers, Kinda, Minoza, Alida, Kauffmann-Lacroix, Catherine, Guégan, Hélène, Costa, Damien, Dehais, Marion, Gargala, Gilles, Bajolet, Odile, Banisadr, Firouze, Cousson, Joel, Himberlin, Chantal, Huguenin, Antoine, Toubas, Dominique, Flori, Pierre, Mahinc, Caroline, Raberin, Hélène, Denis, Julie, Herbrecht, Raoul, Mertes, Paul-Michel, Meziani, Fehrat, Sabou, Marcela, Schneider, Francis, Bertozzi, Anne-Isabelle, Chauvin, Pamela, Charpentier, Elena, Cointault, Olivier, Cottrel, Claire, Delavigne, Karen, Faguer, Stanislas, Fillaux, Judith, Guemas, Emilie, Iriart, Xavier, Lelièvre, Lucie, Ruiz, Jean, Bastides, Frédéric, Chesnay, Adélaïde, Desoubeaux, Guillaume, Therby, Audrey, Chachaty, Elisabeth, Gachot, Bertrand, Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Software and Databasing, Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals (CNRMA), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Dynamique des interactions hôte pathogène (DIHP), Université de Strasbourg (UNISTRA), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Nantes Université (Nantes Univ), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre Hospitalier Universitaire de Nice (CHU Nice), CHU Rouen, Normandie Université (NU), Centre hospitalier universitaire de Poitiers (CHU Poitiers), Ecologie et biologie des interactions (EBI), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), CHU Clermont-Ferrand, CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), CHU de la Martinique [Fort de France], CHU Amiens-Picardie, Hôpital Cochin [AP-HP], CHU Limoges, SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques (ICAT), Université d'Angers (UA), Laboratoire de Parasitologie-Mycologie (CHU d'Angers), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Infections Respiratoires Fongiques (IRF), Université d'Angers (UA)-Université de Brest (UBO), Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre Hospitalier de Versailles André Mignot (CHV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Hôpital Robert Debré Paris, Hôpital Robert Debré, Westerdijk Fungal Biodiversity Institute [Utrecht] (WI), Royal Netherlands Academy of Arts and Sciences (KNAW), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Agents infectieux, résistance et chimiothérapie - UR UPJV 4294 (AGIR ), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Simplification des soins chez les patients complexes - UR UPJV 7518 (SSPC), Université de Picardie Jules Verne (UPJV), Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Laboratoire de Parasitologie-Mycologie [CHU Angers], Hôpital Bretonneau, Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by recurrent financial support from Santé Publique France and Institut Pasteur. The funders had no role in study design, data collection, analysis, interpretation of data, or the decision to submit the work for publication., We thank Didier Che (Direction des Maladies Infectieuses, Santé Publique France) for his continuous support and interest in invasive fungal infections and Etienne Sevin (EpiConcept, Paris, France) for his contribution in the development of the RESSIF website using the VOOZANOO platform. We are thankful for the technical help of Catherine Blanc, Anne Boullié, Cécile Gautier, Virginie Geolier, and Damien Hoinard (Institut Pasteur) for the characterization of the isolates received at the NRCMA., The French Mycoses Study Group includes collaborators who contributed to these data by their involvement in the management of patients, expertise in diagnostic tools, and/or characterization of fungal isolates. They are listed in alphabetical order of a city in France and for each center, in alphabetical order of the last names: in Amiens, Céline Damiani, Hervé Dupont, Julien Maizel, and Anne Totet, in Angers, Jean-Philippe Bouchara, in Besançon, Anne Pauline Bellanger, Ana Berceanu, Jean Christophe Navellou, and Emeline Scherer, in Cayenne, Philippe Abboud, Christine Aznar, Denis Blanchet, and Félix Djossou, in Clermont-Ferrand, Sophie Cayot, Cyril Garrouste, Olivier Lesens, Cécile Moluçon, Céline Nourrisson, and Philippe Poirier, in Dijon, Éloïse Bailly, Louise Basmaciyan, Bouhemad Belaid, Mathieu Blot, Denis Caillot, Pierre-Emmanuel Charles, and Jean Pierre Quenot, in Fort de France, Emmanuelle Amazan, Emilie Baubion, André Cabie, Cyrille Chabartier, Christophe Deligny, Violaine Emal, Olivier Flechelle, Yves Hatchuel, Yohann Le Govic, Harold Merle, Charline Miossec, Jean-Marie Turmel, and Ruddy Valentino, in Limoges, Marie-Fleur Durieux and Pascal Turlure, in Nîmes, Victor Mercier, in Nice, Martine Gari-Toussaint, Karine Risso, and Loïc Simon, in Nantes, Cedric Bretonnière, David Boutoille, Thomas Gastinne, Karim Lakhal, Elise Launay, Thierry Lepoivre, Pierre Peterlin, Fanny Rialland, and Patrice Le Pape, in Paris, in Hôpital Cochin, Etienne Canoui, Naima Dahane, and Solène Kerneis, in Hôpital Necker-Enfants Malades, Cécile Angebault, Marie-Elisabethh Bougnoux, Nadia Guennouni, Fanny Lanternier, Bennedicte Neven, Mehdi Oualha, Anne Scemla, Emilie Sitterlé, Felipe Suarez, and Julie Toubiana, in Hôpital Robert-Debré, Stéphane Bonacorci, in Hôpital Saint-Louis, Alexandre Alanio, Anne Bergeron, Blandine Denis, Maud Gits-Muselli, Samia Hamane, and Sophie Touratier, in hôpital des XV-XX, Christine Chaumeil and Lilia Merabet, in Pointe-à-Pitre, Joelle Claudéon, Elodie Curlier, Valérie Galantine, Jean Claude Gallois, Samuel Markowicz, Muriel Nicolas, Pascal Musson, and Kinda Schepers, in Poitiers, Alida Minoza and Catherine Kauffmann-Lacroix, in Rennes, Hélène Guégan, in Rouen, Damien Costa, Marion Dehais, and Gilles Gargala, in Reims, Odile Bajolet, Firouze Banisadr, Joel Cousson, Chantal Himberlin, Antoine Huguenin, and Dominique Toubas, in Saint Etienne, Pierre Flori, Caroline Mahinc, and Hélène Raberin, in Strabourg, Julie Denis, Raoul Herbrecht, Paul-Michel Mertes, Fehrat Meziani, Marcela Sabou, and Francis Schneider, in Toulouse, Anne-Isabelle Bertozzi, Pamela Chauvin, Elena Charpentier, Olivier Cointault, Claire Cottrel, Karen Delavigne, Stanislas Faguer, Judith Fillaux, Emilie Guemas, Xavier Iriart, Lucie Lelièvre, and Jean Ruiz, in Tours, Frédéric Bastides, Adélaïde Chesnay, and Guillaume Desoubeaux, in Versailles, Audrey Therby, and in Villejuif, Elisabeth Chachaty and Bertrand Gachot., and CLEMENT, Nathalie

Subjects
Antifungal Agents, pneumocytosis, invasive fungal infections, Pneumonia, Pneumocystis, [SDV]Life Sciences [q-bio], candidemia, Microbiology, [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, mucormycosis, [SDV] Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Virology, Humans, aspergillosis, epidemiology, Watchful Waiting, Fungemia, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Aged
Abstract

International audience; The French National Reference Center for Invasive Mycoses and Antifungals leads an active and sustained nationwide surveillance program on probable and proven invasive fungal diseases (IFDs) to determine their epidemiology in France. Between 2012 and 2018, a total of 10,886 IFDs were recorded. The incidence increased slightly over time (2.16 to 2.36/10,000 hospitalization days, P = 0.0562) in relation with an increase of fungemia incidence (1.03 to 1.19/10,000, P = 0.0023), while that of other IFDs remained stable. The proportion of ≥65-year-old patients increased from 38.4% to 45.3% (P < 0.0001). Yeast fungemia (n = 5,444) was due mainly to Candida albicans (55.6%) with stable proportions of species over time. Echinocandins became the main drug prescribed (46.7% to 61.8%), but global mortality rate remained unchanged (36.3% at 1 month). Pneumocystis jirovecii pneumonia (n = 2,106) was diagnosed mostly in HIV-negative patients (80.7%) with a significantly higher mortality than in HIV-positive patients (21.9% versus 5.4% at 1 month, P < 0.0001). Invasive aspergillosis (n = 1,661) and mucormycosis (n = 314) were diagnosed mostly in hematology (>60% of the cases) with a global mortality rate of 42.5% and 59.3%, respectively, at 3 months and significant changes in diagnosis procedure over time. More concurrent infections were also diagnosed over time (from 5.4% to 9.4% for mold IFDs, P = 0.0115). In conclusion, we observed an aging of patients with IFD with a significant increase in incidence only for yeast fungemia, a trend toward more concurrent infections, which raises diagnostic and therapeutic issues. Overall, global survival associated with IFDs has not improved despite updated guidelines and new diagnostic tools.IMPORTANCE The epidemiology of invasive fungal diseases (IFDs) is hard to delineate given the difficulties in ascertaining the diagnosis that is often based on the confrontation of clinical and microbiological criteria. The present report underlines the interest of active surveillance involving mycologists and clinicians to describe the global incidence and that of the main IFDs. Globally, although the incidence of Pneumocystis pneumonia, invasive aspergillosis, and mucormycosis remained stable over the study period (2012 to 2018), that of yeast fungemia increased slightly. We also show here that IFDs seem to affect older people more frequently. The most worrisome observation is the lack of improvement in the global survival rate associated with IFDs despite the increasing use of more sensitive diagnostic tools, the availability of new antifungal drugs very active in clinical trials, and a still low/marginal rate of acquired in vitro resistance in France. Therefore, other tracks of improvement should be investigated actively.

Published
2022

27. Good use of fluoroquinolones by generalpractitioners of quality groups in Guadeloupe

Author

Ducasse, Maxime, BHU, Pointe à Pitre, Université des Antilles - UFR des sciences médicales Hyacinthe Bastaraud (UA UFR SM), Université des Antilles (UA), Kinda Schepers, and Bénédicte Melot

Subjects
Antibiotiques -- Prescription médicale -- Guadeloupe, [SDV] Life Sciences [q-bio], [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Antibiothérapie -- Guadeloupe, [SDV]Life Sciences [q-bio], Médecins généralistes -- Guadeloupe, Connaissances en santé -- Guadeloupe, Attitudes et pratique en santé -- Guadeloupe, MESH: Connaissances, attitudes et pratique en santé -- Guadeloupe, MESH: Résistance bactérienne aux médicaments -- Guadeloupe, MESH: Médecins généralistes -- Guadeloupe, Résistance aux antibiotiques -- Guadeloupe, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

Introduction: In 2015 some estimations evaluated that 30% to 50% of antibiotic prescriptions in France were not conformed to the latest guidelines for good practice of antibiotic use. The resistance to antibiotics is a worldwide threat to public health, with a negative impact in patient outcomes. Our aim was to identify the determining factors in the prescription of fluoroquinolones (FQ) by the general practitioners (GP) in Guadeloupe (indications, choice of the molecule, dosage, duration and adaptation) and make an inventory of this prescription before and after corrective intervention. Methods: We realized a qualitative survey of the determinants of the prescription of FQ by the general practitioners of the quality groups in Guadeloupe in 2016. We then conducted a descriptive case-control study before and after corrective intervention, focused on the average number of prescribed boxes of FQ by these practitioners,during the first semester of 2016 and 2017. Results: Of the 94 physicians to include, 51 participated in the study. Of the 30 analyzable records, 90% (n = 27) of FQ prescriptions were for urinary tract disease with major use of Ofloxacin. Only 4 cards out of the 30 were fully compliant with FQ's policy of good practice, with 18 questionable indications and 15 nonconformities for the duration of prescription. For the descriptive study before and after corrective intervention, the FQ prescription of 86 doctors of the Guadeloupe quality groups could be compared to that of a control group with 199 GPs from Guadeloupe. Thus,we observed that between 2016 and 2017, the quality groups decreased their FQ prescription with an average of 19 to 17boxes per GP per semester whereas an upward trend could be observed in the control group increasing from 27 to 28. The corrective intervention was associated with an improvement in the prescription of fluoroquinolones by the physicians of the quality groups (p = 0.03). Conclusion: Our study showed that GPs of the quality groups used FQ mainly to treat diseases related to the urinary system, which is an approved indication of use in first intention only in case of acute nephritis. Dosages were respected but the duration was too long in half of the cases (n=15). On the basis of this observation, a corrective intervention was carried out and significantly improved the prescription of FQ by the physicians of the quality groups compared to that of the control group, but repeated intervention would probably have a greater impact., Introduction : En 2015, des estimations montraient que 30 à 50 % des antibiothérapies prescrites en France, n’étaient pas conformes aux dernières recommandations du bon usage des antibiotiques. La résistance bactérienne qui en résulte est un problème majeur de santé publique, avec un impact défavorable sur la morbi-mortalité des patients. L’objectif de cette étude était d’identifier les éléments déterminants dans la prescription des fluoroquinolones (FQ) par les médecins généralistes de Guadeloupe(indications, choix de la molécule, posologie, durée et adaptation) et faire un état des lieux de cette prescription avant et après intervention corrective. Méthodes : Il s’agit d’une enquête qualitative des éléments déterminants la prescription de FQ de janvier 2016 à juin 2017 par les médecins généralistes des groupes qualité de Guadeloupe,suivie d’une étude descriptive cas-témoins avant/après intervention corrective,centrée sur le nombre moyen semestriel de boites prescrites de FQ chez ces médecins au premier semestre 2016 et 2017. Résultats : Sur les 94 médecins à inclure, 54% (n=51) ont participé à l’étude. Sur les 30 fiches analysables, 90% (n=27) des prescriptions de FQ avaient pour principale indication une pathologie urinaire avec une utilisation prédominante d’Ofloxacine. Seules 4 fiches sur les 30 étaient entièrement conformes à la politique de bon usage des FQ, avec 18 indications discutables et 15 non-conformités pour la durée de prescription. Pour l’étude descriptive avant/après intervention corrective, on a pu observer qu’entre 2016 et 2017, les groupes qualité (86 médecins des groupes qualité) ont diminué leur prescription de FQ avec une moyenne de prescription semestrielle passant de 19 à 17, alors qu’une tendance à la hausse a pu être observée dans le groupe témoin (199 médecins généralistes) avec une moyenne semestrielle passant de 27 à 28. L’intervention corrective a été associée à une amélioration significative de la prescription des fluoroquinolones (p=0.03). Conclusion : Notre étude a montré que les médecins généralistes des groupes qualité utilisaient les FQ principalement pour traiter des pathologies en rapport avec l’appareil urinaire, ce qui représente une bonne indication en première intention uniquement pour les pyélonéphrites simples. Les posologies ont été respectées, mais la durée, elle, a été trop longue dans la moitié des cas (n=15). En partant de ce constat, une intervention corrective a été réalisée et a permis, une amélioration significative de la prescription des FQ par les médecins des groupes qualité par rapport à celle du groupe témoin qui devra surement être répétée pour obtenir des résultats pérennes.

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results