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Item does not contain fulltext, Telomeres are repetitive DNA structures that, together with the shelterin and the CST complex, protect the ends of chromosomes. Telomere shortening is mitigated in stem and cancer cells through the de novo addition of telomeric repeats by telomerase. Telomere elongation requires the delivery of the telomerase complex to telomeres through a not yet fully understood mechanism. Factors promoting telomerase-telomere interaction are expected to directly bind telomeres and physically interact with the telomerase complex. In search for such a factor we carried out a SILAC-based DNA-protein interaction screen and identified HMBOX1, hereafter referred to as homeobox telomere-binding protein 1 (HOT1). HOT1 directly and specifically binds double-stranded telomere repeats, with the in vivo association correlating with binding to actively processed telomeres. Depletion and overexpression experiments classify HOT1 as a positive regulator of telomere length. Furthermore, immunoprecipitation and cell fractionation analyses show that HOT1 associates with the active telomerase complex and promotes chromatin association of telomerase. Collectively, these findings suggest that HOT1 supports telomerase-dependent telomere elongation.

The use of the new ISO/CIE Standard General Sky offers some new challenges for lighting designers. The MAMmodeller package described in this study provides a user-friendly tool for visualizing each of the SSLD 15 models in simple design contexts. The method of aperture meridians (MAM) provides a relatively simple geometric framework to estimate the appearance of the sky as a luminance map in the window opening, as seen from a point of interest inside the room. In addition, the total horizontal illuminance at the same point is estimated. This provides the user or designer with a quick tool for undertaking preliminary design investigations. MAMmodeller is not a complete lighting design tool, it considers only sunlight and the diffuse light component from the sky entering through the window opening. It does not account for internal reflections, artificial lighting, or external reflections.

This paper presents a comparison between some new models for representing sky luminance, as proposed by Kittler, Darula and Perez, and some typical skies recorded in Sydney, Australia. The results of this study show that these new models are most probably better suited to model skies that are clear or fully overcast as compared with the CIE standard sky models. Skies that have partial cloud coverage are typically more complex than can be modelled using standard models. In such cases special local models would have to be devised.