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140 results on '"Kner P"'

1. Roadmap on computational methods in optical imaging and holography [invited]

Author

Rosen, Joseph, Alford, Simon, Allan, Blake, Anand, Vijayakumar, Arnon, Shlomi, Arockiaraj, Francis Gracy, Art, Jonathan, Bai, Bijie, Balasubramaniam, Ganesh M., Birnbaum, Tobias, Bisht, Nandan S., Blinder, David, Cao, Liangcai, Chen, Qian, Chen, Ziyang, Dubey, Vishesh, Egiazarian, Karen, Ercan, Mert, Forbes, Andrew, Gopakumar, G., Gao, Yunhui, Gigan, Sylvain, Gocłowski, Paweł, Gopinath, Shivasubramanian, Greenbaum, Alon, Horisaki, Ryoichi, Ierodiaconou, Daniel, Juodkazis, Saulius, Karmakar, Tanushree, Katkovnik, Vladimir, Khonina, Svetlana N., Kner, Peter, Kravets, Vladislav, Kumar, Ravi, Lai, Yingming, Li, Chen, Li, Jiaji, Li, Shaoheng, Li, Yuzhu, Liang, Jinyang, Manavalan, Gokul, Mandal, Aditya Chandra, Manisha, Manisha, Mann, Christopher, Marzejon, Marcin J., Moodley, Chané, Morikawa, Junko, Muniraj, Inbarasan, Narbutis, Donatas, Ng, Soon Hock, Nothlawala, Fazilah, Oh, Jeonghun, Ozcan, Aydogan, Park, YongKeun, Porfirev, Alexey P., Potcoava, Mariana, Prabhakar, Shashi, Pu, Jixiong, Rai, Mani Ratnam, Rogalski, Mikołaj, Ryu, Meguya, Choudhary, Sakshi, Salla, Gangi Reddy, Schelkens, Peter, Şener, Sarp Feykun, Shevkunov, Igor, Shimobaba, Tomoyoshi, Singh, Rakesh K., Singh, Ravindra P., Stern, Adrian, Sun, Jiasong, Zhou, Shun, Zuo, Chao, Zurawski, Zack, Tahara, Tatsuki, Tiwari, Vipin, Trusiak, Maciej, Vinu, R. V., Volotovskiy, Sergey G., Yılmaz, Hasan, De Aguiar, Hilton Barbosa, Ahluwalia, Balpreet S., and Ahmad, Azeem

Published
2024
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3. Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP- histones.

Author

Matsuda, Atsushi, Shao, Lin, Boulanger, Jerome, Kervrann, Charles, Carlton, Peter M, Kner, Peter, Agard, David, and Sedat, John W

Subjects
Chromosome Structures, Animals, Drosophila, Green Fluorescent Proteins, Histones, Microscopy, Fluorescence, Mitosis, Chromosomes, Insect, Microscopy, Fluorescence, Chromosomes, Insect, General Science & Technology
Abstract

Photoactivated localization microscopy (PALM) and related fluorescent biological imaging methods are capable of providing very high spatial resolutions (up to 20 nm). Two major demands limit its widespread use on biological samples: requirements for photoactivatable/photoconvertible fluorescent molecules, which are sometimes difficult to incorporate, and high background signals from autofluorescence or fluorophores in adjacent focal planes in three-dimensional imaging which reduces PALM resolution significantly. We present here a high-resolution PALM method utilizing conventional EGFP as the photoconvertible fluorophore, improved algorithms to deal with high levels of biological background noise, and apply this to imaging higher order chromatin structure. We found that the emission wavelength of EGFP is efficiently converted from green to red when exposed to blue light in the presence of reduced riboflavin. The photon yield of red-converted EGFP using riboflavin is comparable to other bright photoconvertible fluorescent proteins that allow

Published
2010

6. Photocarrier Relaxation in the Quantum Kinetics Regime

Author

Bar-Ad, S., Kner, P., Sayed, K. El, Chemla, D. S., Goldanskii, Vitalii I., editor, Schäfer, Fritz Peter, editor, Toennies, J. Peter, editor, Lotsch, Helmut K. V., editor, Barbara, Paul F., editor, Fujimoto, James G., editor, Knox, Wayne H., editor, and Zinth, Wolfgang, editor

Published
1996
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11. 25^th Anniversary of STED Microscopy and the 20^th Anniversary of SIM: feature introduction

Author

Kner, Peter, Manley, Suliana, Shechtman, Yoav, and Stallinga, Sjoerd

Abstract

This feature issue commemorating 25 years of STED microscopy and 20 years of SIM is intended to highlight the incredible progress and growth in the field of superresolution microscopy since Stefan Hell and Jan Wichmann published the article Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy in Optics Letters in 1994.

Published
2020

19. Three-dimensional nanoscale localization of point-like objects using self-interference digital holography

Author

Marar, Abhijit and Kner, Peter

Abstract

We propose localizing point-like fluorescent emitters in three dimensions with nanometer precision throughout large volumes using self-interference digital holography (SIDH). SIDH enables imaging of incoherently emitting objects over large axial ranges without refocusing, and single molecule localization techniques allow sub-50 nm resolution in the lateral and axial dimensions. We demonstrate three-dimensional localization with SIDH by imaging 100 and 40 nm fluorescent nanospheres. With 49,000 photons detected, SIDH achieves a localization precision of 5 nm laterally and 40 nm axially. We are able to detect the nanospheres from as few as 13,000 detected photons.

Published
2020

20. Stripe artifact reduction for digital scanned structured illumination light sheet microscopy

Author

Liu, Yang, Lauderdale, James D., and Kner, Peter

Abstract

Light sheet microscopy is an important and widely used method for studying large and semi-opaque biological specimens. One drawback of the approach is that it often results in stripe artifacts due to absorption and scattering in the illumination path. Here we describe a new approach which will effectively mitigate the artifacts in digital scanned light sheet microscopy (DSLM) and digital scanned structured illumination light sheet microscopy (DSLM-SI). We further improve the results of DSLM-SI through a new reconstruction method which achieves clearer reconstructed images. We demonstrate the reduction of stripe artifacts by imaging 156 microns deep into the larval zebrafish central nervous system. The magnitude of stripe artifacts is reduced by an average of 20% across three datasets.

Published
2019

26. Adaptive optics stochastic optical reconstruction microscopy (AO-STORM) by particle swarm optimization

Author

Tehrani, Kayvan F., Zhang, Yiwen, Shen, Ping, and Kner, Peter

Abstract

Stochastic optical reconstruction microscopy (STORM) can achieve resolutions of better than 20nm imaging single fluorescently labeled cells. However, when optical aberrations induced by larger biological samples degrade the point spread function (PSF), the localization accuracy and number of localizations are both reduced, destroying the resolution of STORM. Adaptive optics (AO) can be used to correct the wavefront, restoring the high resolution of STORM. A challenge for AO-STORM microscopy is the development of robust optimization algorithms which can efficiently correct the wavefront from stochastic raw STORM images. Here we present the implementation of a particle swarm optimization (PSO) approach with a Fourier metric for real-time correction of wavefront aberrations during STORM acquisition. We apply our approach to imaging boutons 100 μm deep inside the central nervous system (CNS) of Drosophila melanogaster larvae achieving a resolution of 146 nm.

Published
2017

34. VCSELs Go the Distance

Author

Kner, P., primary, Sun, D., additional, Boucart, J., additional, Floyd, P., additional, Nabiev, R., additional, Davis, D., additional, Yuen, W., additional, Jansen, M., additional, and Chang-Hasnain, C. J., additional

Published
2002
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38. High-performance 1.6 [micro sign]m single-epitaxy top-emitting VCSEL

Author

Yuen, W., primary, Li, G.S., additional, Nabiev, R.F., additional, Boucart, J., additional, Kner, P., additional, Stone, R.J., additional, Zhang, D., additional, Beaudoin, M., additional, Zheng, T., additional, He, C., additional, Yu, K., additional, Jansen, M., additional, Worland, D.P., additional, and Chang-Hasnain, C.J., additional

Published
2000
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