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Copyright of Czecho-Slovak Pediatrics / Česko-Slovenská Pediatrie is the property of Czech Medical Association of JE Purkyne and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In the presence of amino sugars d-glucosamine and d-galactosamine no spontaneous competence could be observed in the highly transformable R6bd strain of Pneumococcus or it was decreased by several orders of magnitude. The highest inhibition of competence was detected when the amino sugar at a concentration 5 mg/ml of the medium was added not only to the transformation but also to the pretransformation medium. After a 150 min growth in the transformation medium in the presence of the amino sugar a 3-4-fold greater number of cells (as a viable count) could be detected as compared with the control without the amino sugar. It was found microscopically that the amino sugar prevents natural agglutination, which normally occurs in the competent culture. The role of specific amino sugar determinants for binding of the competence factor on the cell surface and the resulting inhibitory effect of these sugars on the development of competence are discussed. [ABSTRACT FROM AUTHOR]

The binding of the competence factor to cellular receptors of physiologically non-competent cells of Pneumococcus was followed as a function of time. A transformation medium without bovine serum albumin was used to study the binding of the competence factor. Control cells without the added factor remained completely non-competent under these conditions. The maximal binding of the factor to the cellular receptors took place already after 3 min of contact of the cells with the factor at 37°C. After 10 min, when the maximum induction of competence occurs in the system used, the competence factor is fully released from the receptors to the medium. It follows that within the period between the 3rd and 10th min, when the cells are being modified for the irreversible binding of DNA, the presence of the competence factor on the cells is no longer necessary. [ABSTRACT FROM AUTHOR]

Genetic transformation and transfection of lysozyme-treated Bacillus subtilis spheroplasts 168M ind occurs only if they are stabilized with 0.5 m phosphate buffer and not if they are stabilized with 0.5 m sucrose. Spheroplasts prepared from maximally competent cells give maximum transformation and transfection results. The results indicate that the DNA receptors must also be intact in the spheroplasts. [ABSTRACT FROM AUTHOR]