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15 results on '"Kuijk, Carlijn"'

2. Generation of human ILC3 from allogeneic and autologous CD34+ hematopoietic progenitors toward adoptive transfer

Author

Van der Meer, Jolien M.R., primary, Bulder, Ingrid, additional, Kuijk, Carlijn, additional, Kleijer, Marion, additional, Verheij, Myrddin W., additional, Omar, Said Z., additional, Haverkate, Nienke J.E., additional, Dolstra, Harry, additional, Blom, Bianca, additional, Hazenberg, Mette D., additional, and Voermans, Carlijn, additional

Published
2023
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6. Impact of cholesterol on proinflammatory monocyte production by the bone marrow

Author

Stiekema, Lotte C A, primary, Willemsen, Lisa, additional, Kaiser, Yannick, additional, Prange, Koen H M, additional, Wareham, Nicholas J, additional, Boekholdt, S Matthijs, additional, Kuijk, Carlijn, additional, de Winther, Menno P J, additional, Voermans, Carlijn, additional, Nahrendorf, Matthias, additional, Stroes, Erik S G, additional, and Kroon, Jeffrey, additional

Published
2021
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10. Differences between Platelets Derived from Neonatal Cord Blood and Adult Peripheral Blood Assessed by Mass Spectrometry

Author

Stokhuijzen, Eva, Koornneef, Johanna M, Nota, Benjamin, van den Eshof, Bart Laurens, van Alphen, Floris Pieter Joachim, van den Biggelaar, Maartje, Van Der Zwaan, Carmen, Kuijk, Carlijn, Mertens, Koen, Fijnvandraat, Karin, Meijer, Alexander Benjamin, Afd Pharmaceutics, Pharmaceutics, Biomolecular Mass Spectrometry and Proteomics, Graduate School, ARD - Amsterdam Reproduction and Development, ACS - Amsterdam Cardiovascular Sciences, Paediatric Infectious Diseases / Rheumatology / Immunology, ACS - Pulmonary hypertension & thrombosis, Afd Pharmaceutics, Pharmaceutics, and Biomolecular Mass Spectrometry and Proteomics

Subjects
Adult, Blood Platelets, Male, Proteomics, 0301 basic medicine, Platelet Aggregation, proteome, Integrin, 030204 cardiovascular system & hematology, Biochemistry, Umbilical cord, Flow cytometry, 03 medical and health sciences, Thromboxane A2, chemistry.chemical_compound, 0302 clinical medicine, medicine, Humans, Receptor, PAR-1, Platelet, Receptor, mass spectrometry, chemistry.chemical_classification, medicine.diagnostic_test, biology, Chemistry, Infant, Newborn, General Chemistry, Middle Aged, Fetal Blood, Platelet Activation, Molecular biology, 030104 developmental biology, medicine.anatomical_structure, Cord blood, platelets, cord blood, biology.protein, Female, Receptors, Thrombin, Collagen, Transcriptome, Glycoprotein
Abstract

It has been proposed that differences may exist between umbilical cord blood (CB) platelets and adult peripheral blood (APB) platelets, including altered protein levels of the main platelet integrins. We have now compared the protein expression profiles of CB and APB platelets employing a label-free comparative proteomics approach. Aggregation studies showed that CB platelets effectively aggregate in the presence of thromboxane A2 analogue, collagen, and peptide agonists of the proteinase-activated receptors 1 and 4. In agreement with previous studies, higher concentrations of the agonists were required to initiate aggregation in the CB platelets. Mass spectrometry analysis revealed no significant difference in the expression levels of critical platelet receptors like glycoprotein (GP)Ib, GPV, GPIX, and integrin αIIbβ3. This was confirmed using flow cytometry-based approaches. Gene ontology enrichment analysis revealed that elevated proteins in CB platelets were in particular enriched in proteins contributing to mitochondrial energy metabolism processes. The reduced proteins were enriched in proteins involved in, among others, platelet degranulation and activation. In conclusion, this study reveals that the CB and APB platelets are distinct. In particular, changes were observed for proteins that belong to metabolic and energy generation processes and not for the critical adhesive platelet integrins and glycoproteins.

Published
2017

11. Differences between Platelets Derived from Neonatal Cord Blood and Adult Peripheral Blood Assessed by Mass Spectrometry

Author

Stokhuijzen, Eva, Koornneef, Johanna Maria, Nota, Benjamin, van den Eshof, Bart Laurens, van Alphen, Floris Pieter Joachim, van den Biggelaar, Maartje, van der Zwaan, Carmen, Kuijk, Carlijn, Mertens, Koen, Fijnvandraat, Karin, and Meijer, Alexander Benjamin

Abstract

It has been proposed that differences may exist between umbilical cord blood (CB) platelets and adult peripheral blood (APB) platelets, including altered protein levels of the main platelet integrins. We have now compared the protein expression profiles of CB and APB platelets employing a label-free comparative proteomics approach. Aggregation studies showed that CB platelets effectively aggregate in the presence of thromboxane A2 analogue, collagen, and peptide agonists of the proteinase-activated receptors 1 and 4. In agreement with previous studies, higher concentrations of the agonists were required to initiate aggregation in the CB platelets. Mass spectrometry analysis revealed no significant difference in the expression levels of critical platelet receptors like glycoprotein (GP)Ib, GPV, GPIX, and integrin αIIbβ3. This was confirmed using flow cytometry-based approaches. Gene ontology enrichment analysis revealed that elevated proteins in CB platelets were in particular enriched in proteins contributing to mitochondrial energy metabolism processes. The reduced proteins were enriched in proteins involved in, among others, platelet degranulation and activation. In conclusion, this study reveals that the CB and APB platelets are distinct. In particular, changes were observed for proteins that belong to metabolic and energy generation processes and not for the critical adhesive platelet integrins and glycoproteins.

Published
2024
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12. Interferon-Gamma Impairs Maintenance and Alters Hematopoietic Support of Bone Marrow Mesenchymal Stromal Cells

Author

Goedhart, Marieke, primary, Cornelissen, Anne S., additional, Kuijk, Carlijn, additional, Geerman, Sulima, additional, Kleijer, Marion, additional, van Buul, Jaap D., additional, Huveneers, Stephan, additional, Raaijmakers, Marc H.G.P., additional, Young, Howard A., additional, Wolkers, Monika C., additional, Voermans, Carlijn, additional, and Nolte, Martijn A., additional

Published
2018
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15. Increased haematopoietic activity in patients with atherosclerosis.

Author

van der Valk, Fleur M., Kuijk, Carlijn, Verweij, Simone L., Stiekema, Lotte C.A., Kaiser, Y., Zeerleder, Sacha, Nahrendorf, Matthias, Voermans, Carlijn, and Stroes, Erik S.G.

Abstract

Aims: Experimental work posits that acute ischaemic events trigger haematopoietic activity, driving monocytosis, and atherogenesis. Considering the chronic low-grade inflammatory state in atherosclerosis, we hypothesized that haematopoietic hyperactivity is a persistent feature in cardiovascular disease (CVD). Therefore, we aimed to assess the activity of haematopoietic organs and haematopoietic stem and progenitor cells (HSPCs) in humans. Methods and results: First, we performed 18F-fluorodeoxyglucose positron emission tomographic (18F-FDG PET) imaging in 26 patients with stable atherosclerotic CVD (ischaemic event >12 months ago), and 25 matched controls. In splenic tissue, 18F-FDG uptake was 2.68+0.65 in CVD patients vs. 1.75+0.54 in controls (1.6-fold higher; P < 0.001), and in bone marrow 3.20+0.76 vs. 2.72+0.46 (1.2-fold higher; P = 0.003), closely related to LDL cholesterol levels (LDLc, r = 0.72). Subsequently, we determined progenitor potential of HSPCs harvested from 18 patients with known atherosclerotic CVD and 30 matched controls; both groups were selected from a cohort of cancer patients undergoing autologous stem cell transplantation. In CVD patients, the normalized progenitor potential, expressed as the number of colony-forming units-granulocyte/monocyte (CFU-GM) colonies/CD34+ cell, was 1.6-fold higher compared with matched controls (P < 0.001). Finally, we assessed the effects of native and oxidized lipoproteins on HSPCs harvested from healthy donors in vitro. Haematopoietic stem and progenitor cells displayed a 1.5-fold increased CFU-GM capacity in co-culture with oxidized LDL in vitro (P = 0.002), which was inhibited by blocking oxidized phospholipids via E06 (P = 0.001). Conclusion: Collectively, these findings strengthen the case for a chronically affected haematopoietic system, potentially driving the low-grade inflammatory state in patients with atherosclerosis. [ABSTRACT FROM AUTHOR]

Published
2017
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