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Objective: Over the years, the roles of microRNAs (miRNAs) and histone deacetylase 3 (HDAC3) in human diseases have been investigated. This study focused on the effect of miR‐19a‐3p and HDAC3 in myocardial ischemia–reperfusion (I/R) injury (MIRI) by targeting cyclin‐dependent kinase 2 (CDK2). Methods: The I/R rat models were established by coronary artery ligation, which were then treated with RGFP966 (an inhibitor of HDAC3), miR‐19a‐3p agomir or antagomir, or silenced CDK2 to explore their roles in the cardiac function, pathological changes of myocardial tissues, myocardial infarction area, inflammatory factors and oxidative stress factors in rats with MIRI. The expression of miR‐19a‐3p, HDAC3, and CDK2 was determined by RT‐qPCR and western blot assay, and the interaction among which was also verified by online prediction, luciferase activity assay and ChIP assay. Results: The results indicated that HDAC3 and CDK2 were upregulated while miR‐19a‐3p was downregulated in myocardial tissues of I/R rats. The inhibited HDAC3/CDK2 or elevated miR‐19a‐3p could promote cardiac function, attenuate pathological changes, inflammatory reaction, oxidative stress, myocardial infarction area and apoptosis of myocardial tissues. HDAC3 mediates miR‐19a‐3p and CDK2 is targeted by miR‐19a‐3p. Conclusion: Inhibited HDAC3 ameliorates MIRI in a rat model by elevating miR‐19a‐3p and reducing CDK2, which may contribute to the treatment of MIRI. [ABSTRACT FROM AUTHOR]

The present study aimed to measure the levels of microRNA-381 (miR-381) in the plaque tissues, peripheral blood mononuclear cells (PBMCs) and serum of patients with coronary atherosclerosis. In addition, the regulatory mechanisms of miR-381 and cyclooxygenase (COX)-2 in coronary atherosclerosis were investigated. A total of 36 patients with coronary atherosclerosis who received coronary endarterectomy at Linyi People's Hospital and Junan Hospital of Traditional Chinese Medicine (Linyi, China) between January 2013 and June 2016 were enrolled into the present study, while 39 healthy subjects were included as the control group. Peripheral blood was collected form all patients and healthy subjects. Plaque tissues were resected from patients with coronary atherosclerosis and adjacent artery intimal tissues were resected as the control tissues. Using quantitative polymerase chain reaction, the levels of miR-381 and COX-2 mRNA in the plaque tissues, PBMCs and serum were determined. In addition, COX-2 protein expression in the plaque tissues and PBMCs was measured by western blotting, while enzyme-linked immunosorbent assay was utilized to examine the protein content in the serum. To identify the direct interaction between miR-381 and COX-2 mRNA, dual-luciferase reporter assay was also conducted. The levels of COX-2 mRNA and protein in the plaque tissues, PBMCs and serum of patients with coronary atherosclerosis were significantly elevated compared with those in the corresponding control groups. However, the expression of miR-381 was significantly reduced in the coronary atherosclerosis patients. Dual-luciferase reporter assay revealed that miR-381 was able to directly target the 3′-untranslated region of COX-2 mRNA to regulate the expression of COX-2. Therefore, the present study demonstrated that enhanced levels of COX-2 expression in patients with coronary atherosclerosis are associated with the downregulation of miR-381 expression, while miR-381 may regulate the occurrence and immune responses of coronary atherosclerosis via COX-2. [ABSTRACT FROM AUTHOR]

Objectives: In the present study, we evaluated the mechanical outcome of different configurations formed by partially threaded screws (PTS) alone or combined screws consisting of PTS and fully threaded screws (FTS) in the treatment of unstable femoral neck fracture.Methods: The Pauwels type III unstable femoral fracture and screw models of PTS and FTS were created in 3-matic software and UG-NX software respectively. We assembled the different screw fixation types to the fracture model separately to form the fixation models. We used Abaqus software to perform the finite element analysis.Results: Our results indicated that the peak von Mises stresses of screws increased when some PTSs changed into FTSs in all groups except for the inferior triangle group. FTS in each group underwent the most stress while PTS underwent a little bit of stress. The combined screws fixation types were less likely to be cut-out and was more stable than PTPs alone fixation strategy. Less yielding regions around the screw tunnels for the superior and inferior triangle configuration fixed by combined screws was indicated. Superior triangle fixation model underwent the largest area of stress concentration around the screw holes after screws removal.Conclusions: For unstable femoral neck fractures, superior results were obtained by stabilizing the fracture with superior or inferior triangle configuration consisting of one PTS and two FTSs. If screws removal was taken into account after fracture union, fixation type of inferior triangle configuration should be the recommended choice. [ABSTRACT FROM AUTHOR]

Objectives. In the present study, we evaluated the mechanical outcome of different configurations of cannulated compression screws for the fixation of Pauwels type III femoral neck fracture and the stress distribution around the holes corresponding to fixation protocol after screws removal. Methods. The Pauwels type III of femoral neck fracture was created in 3-matic software and the models of cannulated compression screws were constructed using UG-NX software. Five fixation systems were assembled to the fracture models. Abaqus software was used to perform the process of finite element analysis. Values of stress distribution, maximum stress, model principal strains of proximal fragment, and stress distribution around the holes of femur model were recorded. Results. Stress of cannulated compression screws was intensely focused on the middle area of the screw near the fragment of each group. Inverted triangle model showed the highest peak stress on screws under different phases of load. Each screw dispersed some stresses, but at least one underwent the peak stress. Fracture model fixed by inverted triangle configuration showed the lowest volume of yielding strain in the proximal fragment. The area of higher stress around the holes was largest after triangle screws removal when compared with other four models. Conclusions. Our study indicated that different cannulated compression screws fixation configurations for the unstable femoral neck fractures showed the different mechanical efficiency. Inverted triangular configuration showed the mechanical advantage and being less likely to cutout. The fixation strategy of triangle configuration was least recommended if patients tended to remove the implants. [ABSTRACT FROM AUTHOR]

Objectives: In the present study, we assessed the biomechanical advantage between the single LISS and double-plate used in AO type 33-C2 fractures with the method of finite element analysis, which will help surgeons choose the optimal therapy to the unstable distal femoral fracture.Methods: The AO type 33-C2 fractures and the models of LISS plate and medial plate was constructed in 3-matic software and UG-NX software respectively. We then assembled the single plate and the double-plate to the fracture model separately to form the fixation models. After meshing the models' elements, we used the Abaqus software to perform the finite element analysis. Values of peak Von Mises Stress (VMS) on the plate, maximum deformation of the models and the distance changes of the fracture gap were used to capture the mechanical factors in this study.Results: Our results indicated that the single LISS underwent 1.2 times higher amount of stress than the double-plate (316.0 MPa VS 281.6 MPa). And the medial plate dispersed some stresses (the maximum stress is 47.4 MPa). Single-plate generated 3 times greater bending angle than double-plate (0.6° VS 0.2°). The bending angles of the single and double-fixation-fracture models are 0.9° and 0.3° respectively. The maximum distance changes of the fracture gap in the single-plate model was 2 times higher than that of double-plate model (2.6 mm VS 1.3 mm). In the torsional load analysis, peak VMS of the single and double model was 1.0 MPa and 0.8 MPa respectively. And the bending angle was 0.8° in the single model and 0.4° in the double model.Conclusions: The double-plate is more effective scenario using in the distal femoral fractures, particular in unstable fractures with joint involvement. [ABSTRACT FROM AUTHOR]

Objectives. We evaluated the biomechanical outcome of different plate fixation strategies (the single plate construct, 45° double-plate construct, 90° double-plate construct, 135° double-plate construct, and 180° double-plate construct) used for the fixation of the femoral shaft nonunion with no cortical support opposite the primary lateral plate. This may help surgeons choose the optimal therapy to the femoral shaft nonunion. Methods. The femoral shaft nonunion with no medial support and the models of lateral plate and medial plate was constructed in 3-matic software and UG-NX software, respectively. We then assembled the single plate and different double plates to the fracture model separately to form the fixation models. After meshing the models’ elements, we used the ABAQUS software to perform the finite element analysis. Values of the von Mises Stress (VMS) distribution of the implant, peak VMS, and model displacement and deformation were used to capture the mechanical factors in this study. Results. Our results indicated that the peak von Mises Stress (VMS) of the lateral plate was concentrated in middle surface of the lateral plate near the fragment of each group. The peak VMS was 5201.0 MPa (the single-plate construct), 3490.0 MPa (45° double-plate construct), 1754.0 MPa (90° double-plate construct), 1123.0 MPa (135° double-plate construct), and 816.5 MPa (180° double-plate construct). The additional short plate dispersed some stress leading to the decrease in the peak VMS of the lateral plate. As angle formed by the double plates increased, the dispersed function of the additional plate was becoming obvious. The bending angles of the lateral plate were 18° versus 12° versus 3° versus 2° versus 1° (the single-plate construct versus 45° double-plate construct versus 90° double-plate construct versus 135° double-plate construct versus 180° double-plate construct). Conclusions. Our study indicated that increasing the angle between the plates in a double-plate construct improves the stability of the construct over a single lateral plate when there is no cortical support opposite to the lateral plate. The strongest fixation occurred when the angle between the two plates was greater than ninety degrees. [ABSTRACT FROM AUTHOR]